CN107629961A - A kind of method using breeding wastewater culture microalgae - Google Patents
A kind of method using breeding wastewater culture microalgae Download PDFInfo
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Abstract
The present invention relates to a kind of method using breeding wastewater culture microalgae, it comprises the following steps：1) microalgae is tamed：Microalgae is inoculated into culture medium and cultivated, the breeding wastewater of non-sterilized processing is added in incubation, and the adding proportion of breeding wastewater is stepped up according to the increased number of microalgae；2) microalgae activates：The breeding wastewater of non-sterilized processing is added in the medium, and then the microalgae after domestication is inoculated in the culture medium and is enlarged culture；3) microdisk electrode：Microalgae after activation is inoculated into the breeding wastewater solution of non-sterilized processing, out of doors under the conditions of cultivated, realize cultivation wastewater purification and microalgae grease production.Method of the present invention using breeding wastewater culture microalgae, using non-sterilized processing breeding wastewater out of doors under the conditions of cultivate microalgae, cultivation wastewater purification and microalgae grease production can be realized simultaneously, have that cost is cheap, the advantages of can mass producing.
Description
Technical field
The present invention relates to a kind of microalgae culture method, more particularly to a kind of method using breeding wastewater culture microalgae.
Background technology
Body eutrophication can be caused rich in the nutrients such as nitrogen, phosphorus, its a large amount of discharge in breeding wastewater, algae is with swimming
Biological mad breeding, causes water body oxygen content amount to decline, causes fish and other biological mortality, so as to destroy the ecology of water body
Balance.In recent years, the plant such as cattle farm, which carries out, concentrates a large amount of waste discharges, causes the ecocrisis of many water bodys, it is useless
Method for treating water urgently improves.
Chlorella has the advantages such as photosynthetic efficiency is high, growth rate is fast, strong environmental adaptability and lipid-producing height,
As most potential biodiesel raw material.Generally use chemical fertilizer is small to obtain as culture medium progress bead algal biomass production
Ball algae oil fat, causes chlorella grease not have competitiveness in cost.Cattle Wastewater is used to produce bead algae oil for culture medium
Fat, the dual purpose of Cattle Wastewater purification and chlorella grease production can be realized simultaneously.
Prior art typically using the Cattle Wastewater for having made sterilization treatment, cultivates chlorella, to keep away in room conditions
The microorganism exempted from waste water or environment causes to encroach on to microalgae.However, Cattle Wastewater is sterilized and sterilizing operation processing,
Cost is too high；On the other hand, the growth and breeding of chlorella needs illumination, and indoor culture needs electricity consumption and provides light source, that is, takes
The interior space, increases production cost again.It can be seen that being produced using prior art purifying aquaculture waste water and microalgae grease, do not have
Have an economic benefit.
The content of the invention
Based on this, it is an object of the present invention to provide a kind of method using breeding wastewater culture microalgae, its use without
The breeding wastewater of sterilization treatment out of doors under the conditions of cultivate microalgae, can realize simultaneously cultivation wastewater purification and microalgae grease life
Production, there is the advantages of cost is cheap, can mass produce.
The technical solution adopted by the present invention is as follows：
A kind of method using breeding wastewater culture microalgae, comprise the following steps：
1) microalgae is tamed：Microalgae is inoculated into culture medium and cultivated, non-sterilized processing is added in incubation
Breeding wastewater, and step up according to the increased number of microalgae the adding proportion of breeding wastewater；
2) microalgae activates：The breeding wastewater of non-sterilized processing is added in the medium, then connects the microalgae after domestication
Kind is enlarged culture in the culture medium；
3) microdisk electrode：Microalgae after activation is inoculated into the breeding wastewater solution of non-sterilized processing, out of doors bar
Cultivated under part, realize cultivation wastewater purification and microalgae grease production.
The present invention adds the breeding wastewater of non-sterilized processing by being tamed to microalgae in the medium, and according to
The increased number of microalgae steps up the adding proportion of breeding wastewater, and microalgae can be made gradually to adapt to the cultivation in non-sterilized processing
Grown in waste water, so that it is guaranteed that microalgae remains able to normal growth in the breeding wastewater of non-sterilized processing, it is useless to resist cultivation
Microorganism infringement in water, and can normally produce grease.
The present invention uses outdoor solar irradiation to provide the energy for micro algae growth, use the breeding wastewater of non-sterilized processing for
Micro algae growth provides the nutriments such as nitrogen, phosphorus, and has ensured that the microorganism in breeding wastewater can not to microalgae by microalgae domestication
The step of being encroached on, therefore sterilizing can be saved or the highly energy-consuming such as disinfect, reduce breeding wastewater denitrogenation dephosphorizing into
Originally with the production cost of grease, there is considerable environmental benefit and economic benefit.
Further, described breeding wastewater is Cattle Wastewater；Its ammonia nitrogen concentration is 172.31~190.69mg/L, always
Nitrogen (TN) concentration is 270.27~295.73mg/L, and total nitrogen (TP) concentration is 108.40~123.40mg/L, chemical oxygen consumption (COC)
(COD) it is 2577.44~2608.56mg/L.
Further, the pH value of the breeding wastewater is 8.15~8.18.By by pH value control 8.15~8.18, energy
It is enough effectively to suppress the bacterial growth in breeding wastewater in the case where ensuring microalgae normal growth, avoid bacterium interference microalgae life
It is long.
Further, described microalgae is chlorella.Chlorella is fresh water algae, is adapted to cultivate in breeding wastewater；And
Chlorella can carry out mixotrophic growth, can both utilize the elements such as the nitrogen in breeding wastewater, phosphorus to realize by photosynthesis
Photoautotrophy, heterotrophism directly can be carried out using the organic substance in breeding wastewater again, can so remove nothing in breeding wastewater
Nitrogen, phosphorus in machine thing, and can remove nitrogen, phosphorus in organic matter, can more fully realize the denitrogenation dephosphorizing of breeding wastewater；This
Outside, fat content in chlorella cells is high, and growth and breeding speed is fast, and the cell increment of 20 hours is up to 4 times, Neng Gouji
The earth improves grease yield.
Further, in step 1), described culture medium is BG-11 culture mediums；The inoculation of the microalgae in the medium
Concentration is 0.08~0.10g/L；The cultivation temperature of the microalgae domestication is 23~28 DEG C, and intensity of illumination is 400~1000um
m2·S-1, light application time is 11~12 hours/day, and incubation time is 14~16 days.
Further, in step 1), when the concentration of microalgae reaches 1.0~1.5g/L, start to add not into culture medium
The breeding wastewater of sterilized processing, and 5% culture medium is entered line replacement by every two days with isometric breeding wastewater, until cultivation
The volumetric concentration of waste water in the medium is 5%~25%.By stepping up the adding proportion of breeding wastewater in the medium,
It can ensure that microalgae, being capable of normal growth after breeding wastewater is inoculated into.
Further, in step 2), described culture medium is BG-11 culture mediums；The inoculation of the microalgae in the medium
Concentration is 0.15~0.20g/L；The cultivation temperature of the microalgae activation is 23~28 DEG C, and intensity of illumination is 400~1000um
m2·S-1, light application time is 11~12 hours/day, and incubation time is 8~12 days., can be true by the activation culture of 8~12 days
The cell quantity and cytoactive for protecting microalgae reach purification of waste water and the inoculum density requirement of grease production.
Further, in step 2), the volumetric concentration of the breeding wastewater in the medium is 5%~25%.
Further, in step 3), described breeding wastewater solution is the aqueous solution containing the breeding wastewater, described to support
Volumetric concentration of the waste water in the aqueous solution is grown for 5%~25%.By by the control of the volumetric concentration of breeding wastewater 5%~
25%, it can be ensured that at the end of culture, nitrogen, phosphorus in breeding wastewater are efficiently removed, while are also able to ensure that in breeding wastewater
There are enough nutriments, meet the growth demand of microalgae.
Further, in step 3), inoculum density of the microalgae in the breeding wastewater solution be 0.17~
0.34g/L.At the end of the inoculum density is able to ensure that culture, by the microalgae of number generation breeding, the de- of breeding wastewater can be completed
Nitrogen, dephosphorization purification task, and can make substantial amounts of microalgae cell keep good activity, avoid microalgae cell from entering the decline stage,
And the grease of itself of degrading, cause grease yield to reduce.
Further, in step 3), the incubation time of the microalgae is 6~10 days, preferably 8 days.During by that will cultivate
Between control at 6~10 days, the nitrogen being on the one hand able to ensure that in breeding wastewater, phosphorus are efficiently removed, while also ensuring that the oil of microalgae
Fat yield reaches maximization.
Embodiment
Embodiment 1
Cattle Wastewater is taken, by precipitation overnight, then by filtered through gauze, takes supernatant standby.Its water quality index is such as
Under：PH value is 8.18, and total nitrogen (TN) concentration is 295.73mg/L, and total phosphorus (TP) concentration is 123.40mg/L, chemical oxygen consumption (COC)
(COD) it is 2608.56mg/L, suspension (SS) is 1.74.
Chlorella algae kind is taken, is inoculated into 0.10g/L concentration in BG-11 culture mediums, is 25 DEG C in environment temperature, illumination
Intensity is 800umm2·S-1, light application time is domestication culture 15 days under conditions of 12 hours/day, in incubation, when
When the concentration of chlorella reaches 1.5g/L, start to add the Cattle Wastewater of non-sterilized processing into culture medium, and every two days
5% culture medium is entered into line replacement with isometric breeding wastewater, until the volumetric concentration of breeding wastewater in the medium is
25%.After domestication culture terminates, BG-11 culture mediums are separately taken, add the milk cow for the non-sterilized processing that final volume concentration is 25%
Chlorella after domestication, is then inoculated into the culture medium by field waste water with 0.20g/L concentration, is 25 DEG C in environment temperature,
Intensity of illumination is 800umm2·S-1, light application time is expansion culture 12 days under conditions of 12 hours/day.Expand culture to terminate
Afterwards, the Cattle Wastewater solution that final volume concentration is 25% is added in pillar bioreactor, will be enlarged by what culture obtained
Chlorella is inoculated into the Cattle Wastewater solution with 0.34g/L concentration, is passed through air and is provided inorganic carbon source simultaneously for chlorella
Chlorella is in suspended state, the pillar bioreactor is then placed in open air, cultivates 8 under illumination condition out of doors
My god, and the biomass variety of chlorella and the water quality index of Cattle Wastewater are monitored daily.
After culture in 8 days, total nitrogen (TN) clearance of Cattle Wastewater is 83.83 ± 1.19%, total phosphorus (TP) clearance
For 100.00 ± 0.00%, chemical oxygen consumption (COC) (COD) clearance 64.82 ± 0.91%；Bead algal biomass obtains lipid-producing
12.60±0.93mg·L-1·day-1(being calculated with fatty acid methyl ester).
Embodiment 2
Cattle Wastewater is taken, by precipitation overnight, then by filtered through gauze, takes supernatant standby.Its water quality index is such as
Under：PH value is 8.18, and total nitrogen (TN) concentration is 295.73mg/L, and total phosphorus (TP) concentration is 123.40mg/L, chemical oxygen consumption (COC)
(COD) it is 2608.56mg/L, suspension (SS) is 1.74.
Chlorella algae kind is taken, is inoculated into 0.08g/L concentration in BG-11 culture mediums, is 25 DEG C in environment temperature, illumination
Intensity is 800umm2·S-1, light application time is domestication culture 15 days under conditions of 12 hours/day, in incubation, when
When the concentration of chlorella reaches 1.0g/L, start to add the Cattle Wastewater of non-sterilized processing into culture medium, and every two days
5% culture medium is entered into line replacement with isometric breeding wastewater, until the volumetric concentration of breeding wastewater in the medium is
5%.After domestication culture terminates, BG-11 culture mediums are separately taken, add the cattle farm for the non-sterilized processing that final volume concentration is 5%
Waste water, then the chlorella after domestication is inoculated into the culture medium with 0.15g/L concentration, is 25 DEG C in environment temperature, light
It is 800umm according to intensity2·S-1, light application time is expansion culture 12 days under conditions of 12 hours/day.Expand culture to terminate
Afterwards, by final volume concentration be 5% Cattle Wastewater solution add pillar bioreactor in, will be enlarged by culture obtain it is small
Ball algae is inoculated into the Cattle Wastewater solution with 0.34g/L concentration, is passed through air and is provided inorganic carbon source for chlorella and make
Chlorella is in suspended state, and the pillar bioreactor then is placed in into open air, is cultivated 8 days under illumination condition out of doors,
And the biomass variety of chlorella and the water quality index of Cattle Wastewater are monitored daily.
After culture in 8 days, total nitrogen (TN) clearance of Cattle Wastewater is 85.12 ± 3.37%, total phosphorus (TP) clearance
For 100.00 ± 0.00%, chemical oxygen consumption (COC) (COD) clearance 85.32 ± 0.78%；Bead algal biomass obtains lipid-producing
4.20±0.15mg·L-1·day-1(being calculated with fatty acid methyl ester).
Embodiment 3
Cattle Wastewater is taken, by precipitation overnight, then by filtered through gauze, takes supernatant standby.Its water quality index is such as
Under：PH value is 8.18, and total nitrogen (TN) concentration is 283.00mg/L, and total phosphorus (TP) concentration is 115.90mg/L, chemical oxygen consumption (COC)
(COD) it is 2593.00mg/L, suspension (SS) is 1.74.
Chlorella algae kind is taken, is inoculated into 0.09g/L concentration in BG-11 culture mediums, is 25 DEG C in environment temperature, illumination
Intensity is 800umm2·S-1, light application time is domestication culture 15 days under conditions of 12 hours/day, in incubation, when
When the concentration of chlorella reaches 1.2g/L, start to add the Cattle Wastewater of non-sterilized processing into culture medium, and every two days
5% culture medium is entered into line replacement with isometric breeding wastewater, until the volumetric concentration of breeding wastewater in the medium is
10%.After domestication culture terminates, BG-11 culture mediums are separately taken, add the milk cow for the non-sterilized processing that final volume concentration is 10%
Chlorella after domestication, is then inoculated into the culture medium by field waste water with 0.18g/L concentration, is 25 DEG C in environment temperature,
Intensity of illumination is 800umm2·S-1, light application time is expansion culture 10 days under conditions of 12 hours/day.Expand culture to terminate
Afterwards, the Cattle Wastewater solution that final volume concentration is 10% is added in pillar bioreactor, will be enlarged by what culture obtained
Chlorella is inoculated into the Cattle Wastewater solution with 0.26g/L concentration, is passed through air and is provided inorganic carbon source simultaneously for chlorella
Chlorella is in suspended state, the pillar bioreactor is then placed in open air, cultivates 8 under illumination condition out of doors
My god, and the biomass variety of chlorella and the water quality index of Cattle Wastewater are monitored daily.
After culture in 8 days, total nitrogen (TN) clearance of Cattle Wastewater is 77.37 ± 1.09%, total phosphorus (TP) clearance
For 100.00 ± 0.00%, chemical oxygen consumption (COC) (COD) clearance 70.76 ± 0.80%；Bead algal biomass obtains lipid-producing
7.78±0.53mg·L-1·day-1(being calculated with fatty acid methyl ester).
Embodiment 4
Cattle Wastewater is taken, by precipitation overnight, then by filtered through gauze, takes supernatant standby.Its water quality index is such as
Under：PH value is 8.18, and total nitrogen (TN) concentration is 283.00mg/L, and total phosphorus (TP) concentration is 115.90mg/L, chemical oxygen consumption (COC)
(COD) it is 2593.00mg/L, suspension (SS) is 1.74.
Chlorella algae kind is taken, is inoculated into 0.10g/L concentration in BG-11 culture mediums, is 25 DEG C in environment temperature, illumination
Intensity is 800umm2·S-1, light application time is domestication culture 15 days under conditions of 12 hours/day, in incubation, when
When the concentration of chlorella reaches 1.5g/L, start to add the Cattle Wastewater of non-sterilized processing into culture medium, and every two days
5% culture medium is entered into line replacement with isometric breeding wastewater, until the volumetric concentration of breeding wastewater in the medium is
25%.After domestication culture terminates, BG-11 culture mediums are separately taken, add the milk cow for the non-sterilized processing that final volume concentration is 25%
Chlorella after domestication, is then inoculated into the culture medium by field waste water with 0.20g/L concentration, is 25 DEG C in environment temperature,
Intensity of illumination is 800umm2·S-1, light application time is expansion culture 10 days under conditions of 12 hours/day.Expand culture to terminate
Afterwards, the Cattle Wastewater solution that final volume concentration is 25% is added in pillar bioreactor, will be enlarged by what culture obtained
Chlorella is inoculated into the Cattle Wastewater solution with 0.26g/L concentration, is passed through air and is provided inorganic carbon source simultaneously for chlorella
Chlorella is in suspended state, the pillar bioreactor is then placed in open air, cultivates 8 under illumination condition out of doors
My god, and the biomass variety of chlorella and the water quality index of Cattle Wastewater are monitored daily.
After culture in 8 days, total nitrogen (TN) clearance of Cattle Wastewater is 73.38 ± 3.25%, total phosphorus (TP) clearance
For 100.00 ± 0.00%, chemical oxygen consumption (COC) (COD) clearance 60.32 ± 1.60%；Bead algal biomass obtains lipid-producing
11.35±0.98mg·L-1·day-1(being calculated with fatty acid methyl ester).
Embodiment 5
Cattle Wastewater is taken, by precipitation overnight, then by filtered through gauze, takes supernatant standby.Its water quality index is such as
Under：PH value is 8.15, and total nitrogen (TN) concentration is 270.27mg/L, and total phosphorus (TP) concentration is 108.40mg/L, chemical oxygen consumption (COC)
(COD) it is 2577.44mg/L, suspension (SS) is 1.74.
Chlorella algae kind is taken, is inoculated into 0.08g/L concentration in BG-11 culture mediums, is 25 DEG C in environment temperature, illumination
Intensity is 800umm2·S-1, light application time is domestication culture 15 days under conditions of 12 hours/day, in incubation, when
When the concentration of chlorella reaches 1.0g/L, start to add the Cattle Wastewater of non-sterilized processing into culture medium, and every two days
5% culture medium is entered into line replacement with isometric breeding wastewater, until the volumetric concentration of breeding wastewater in the medium is
5%.After domestication culture terminates, BG-11 culture mediums are separately taken, add the cattle farm for the non-sterilized processing that final volume concentration is 5%
Waste water, then the chlorella after domestication is inoculated into the culture medium with 0.15g/L concentration, is 25 DEG C in environment temperature, light
It is 800umm according to intensity2·S-1, light application time is expansion culture 8 days under conditions of 12 hours/day.After expansion culture terminates,
The Cattle Wastewater solution that final volume concentration is 5% is added in pillar bioreactor, will be enlarged by the bead that culture obtains
Algae is inoculated into the Cattle Wastewater solution with 0.17g/L concentration, is passed through air and is provided inorganic carbon source for chlorella and make small
Ball algae is in suspended state, and the pillar bioreactor then is placed in into open air, is cultivated 8 days under illumination condition out of doors, and
The water quality index of the biomass variety of monitoring chlorella and Cattle Wastewater daily.
After culture in 8 days, total nitrogen (TN) clearance of Cattle Wastewater is 79.29 ± 3.09%, total phosphorus (TP) clearance
For 100.00 ± 0.00%, chemical oxygen consumption (COC) (COD) clearance 81.21 ± 0.60%；Bead algal biomass obtains lipid-producing
4.40±0.20mg·L-1·day-1(being calculated with fatty acid methyl ester).
Embodiment 6
Cattle Wastewater is taken, by precipitation overnight, then by filtered through gauze, takes supernatant standby.Its water quality index is such as
Under：PH value is 8.15, and total nitrogen (TN) concentration is 270.27mg/L, and total phosphorus (TP) concentration is 108.40mg/L, chemical oxygen consumption (COC)
(COD) it is 2577.44mg/L, suspension (SS) is 1.74.
Chlorella algae kind is taken, is inoculated into 0.09g/L concentration in BG-11 culture mediums, is 25 DEG C in environment temperature, illumination
Intensity is 800umm2·S-1, light application time is domestication culture 15 days under conditions of 12 hours/day, in incubation, when
When the concentration of chlorella reaches 1.2g/L, start to add the Cattle Wastewater of non-sterilized processing into culture medium, and every two days
5% culture medium is entered into line replacement with isometric breeding wastewater, until the volumetric concentration of breeding wastewater in the medium is
10%.After domestication culture terminates, BG-11 culture mediums are separately taken, add the milk cow for the non-sterilized processing that final volume concentration is 10%
Chlorella after domestication, is then inoculated into the culture medium by field waste water with 0.18g/L concentration, is 25 DEG C in environment temperature,
Intensity of illumination is 800umm2·S-1, light application time is expansion culture 8 days under conditions of 12 hours/day.Expand culture to terminate
Afterwards, the Cattle Wastewater solution that final volume concentration is 10% is added in pillar bioreactor, will be enlarged by what culture obtained
Chlorella is inoculated into the Cattle Wastewater solution with 0.17g/L concentration, is passed through air and is provided inorganic carbon source simultaneously for chlorella
Chlorella is in suspended state, the pillar bioreactor is then placed in open air, cultivates 8 under illumination condition out of doors
My god, and the biomass variety of chlorella and the water quality index of Cattle Wastewater are monitored daily.
After culture in 8 days, total nitrogen (TN) clearance of Cattle Wastewater is 77.37 ± 1.09%, total phosphorus (TP) clearance
For 100.00 ± 0.00%, chemical oxygen consumption (COC) (COD) clearance 68.83 ± 0.90%；Bead algal biomass obtains lipid-producing
7.78±0.53mg·L-1·day-1(being calculated with fatty acid methyl ester).
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.
Claims (10)
- A kind of 1. method using breeding wastewater culture microalgae, it is characterised in that comprise the following steps：1) microalgae is tamed：Microalgae is inoculated into culture medium and cultivated, supporting for non-sterilized processing is added in incubation Waste water is grown, and the adding proportion of breeding wastewater is stepped up according to the increased number of microalgae；2) microalgae activates：The breeding wastewater of non-sterilized processing is added in the medium, is then inoculated in the microalgae after domestication Culture is enlarged in the culture medium；3) microdisk electrode：Microalgae after activation is inoculated into the breeding wastewater solution of non-sterilized processing, out of doors under the conditions of Cultivated, realize cultivation wastewater purification and microalgae grease production.
- 2. the method according to claim 1 using breeding wastewater culture microalgae, it is characterised in that：Described breeding wastewater For Cattle Wastewater；Its ammonia nitrogen concentration is 172.31~190.69mg/L, and total nitrogen concentration is 270.27~295.73mg/L, total nitrogen Concentration is 108.40~123.40mg/L, and chemical oxygen consumption (COC) is 2577.44~2608.56mg/L；Its pH value is 8.15~8.18.
- 3. the method according to claim 1 using breeding wastewater culture microalgae, it is characterised in that：Described microalgae is small Ball algae.
- 4. the method using breeding wastewater culture microalgae according to one of claims 1 to 3, it is characterised in that： In step 1), described culture medium is BG-11 culture mediums；The inoculum density of the microalgae in the medium is 0.08~0.10g/ L；The cultivation temperature of the microalgae domestication is 23~28 DEG C, intensity of illumination 400-1000umm2·S-1, light application time 11 ~12 hours/day, incubation time are 14~16 days.
- 5. the method using breeding wastewater culture microalgae according to one of claims 1 to 3, it is characterised in that： In step 1), when the concentration of microalgae reaches 1.0~1.5g/L, the cultivation for starting to add non-sterilized processing into culture medium is given up Water, and 5% culture medium is entered line replacement by every two days with isometric breeding wastewater, until the body of breeding wastewater in the medium Product concentration is 5%~25%.
- 6. the method using breeding wastewater culture microalgae according to one of claims 1 to 3, it is characterised in that： In step 2), described culture medium is BG-11 culture mediums；The inoculum density of the microalgae in the medium is 0.15~0.20g/ L；The cultivation temperature of the microalgae activation is 23~28 DEG C, intensity of illumination 400-1000umm2·S-1, light application time 11 ~12 hours/day, incubation time are 8~12 days.
- 7. the method using breeding wastewater culture microalgae according to one of claims 1 to 3, it is characterised in that： In step 2), the volumetric concentration of the breeding wastewater in the medium is 5%~25%.
- 8. the method using breeding wastewater culture microalgae according to one of claims 1 to 3, it is characterised in that： In step 3), described breeding wastewater solution is the aqueous solution containing the breeding wastewater, and the breeding wastewater is in the aqueous solution In volumetric concentration be 5%~25%.
- 9. the method using breeding wastewater culture microalgae according to one of claims 1 to 3, it is characterised in that： In step 3), inoculum density of the microalgae in the breeding wastewater solution is 0.17~0.34g/L.
- 10. the method using breeding wastewater culture microalgae according to one of claims 1 to 3, it is characterised in that： In step 3), the incubation time of the microalgae is 6~10 days.
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| CN113717854A (en) * | 2021-09-01 | 2021-11-30 | 东北农业大学 | Oil-producing chlorella ZM-5 and application thereof |
| CN113717854B (en) * | 2021-09-01 | 2023-05-02 | 东北农业大学 | Chlorella oleaginous ZM-5 and application thereof |
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| CN114561293B (en) * | 2021-12-24 | 2023-11-28 | 天津大学 | Method for purifying cefradine wastewater based on microalgae culture |
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| CN114763516A (en) * | 2022-03-16 | 2022-07-19 | 河北工业大学 | Method for promoting microalgae carbon sequestration and fatty acid production by using plant hormone under mercury stress of flue gas |
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| CN114890548A (en) * | 2022-05-07 | 2022-08-12 | 广西壮族自治区海洋环境监测中心站 | Method for treating aquaculture tail water |
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