CN107055946A - A kind of method that utilization chlorella purifies pig farm fermentation waste water - Google Patents

A kind of method that utilization chlorella purifies pig farm fermentation waste water Download PDF

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Publication number
CN107055946A
CN107055946A CN201710139296.9A CN201710139296A CN107055946A CN 107055946 A CN107055946 A CN 107055946A CN 201710139296 A CN201710139296 A CN 201710139296A CN 107055946 A CN107055946 A CN 107055946A
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waste water
chlorella
pig farm
purifies
utilization
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CN201710139296.9A
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黄开耀
徐磊
邓璇
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Wuhan Jingyu Microalgae Science & Technology Co ltd
Institute of Hydrobiology of CAS
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Wuhan Jingyu Microalgae Science & Technology Co ltd
Institute of Hydrobiology of CAS
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Priority to CN201710139296.9A priority Critical patent/CN107055946A/en
Publication of CN107055946A publication Critical patent/CN107055946A/en
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
    • C02F1/5245—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents using basic salts, e.g. of aluminium and iron
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F2001/007—Processes including a sedimentation step
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/16—Total nitrogen (tkN-N)
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2301/00—General aspects of water treatment
    • C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F7/00—Aeration of stretches of water

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Biotechnology (AREA)
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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Water Supply & Treatment (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Feed For Specific Animals (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The present invention proposes a kind of method that utilization chlorella purifies pig farm fermentation waste water, comprises the following steps：(1) enter regulating reservoir after the pretreatment of pig farm fermentation waste water and adjust pH=6.5 7.5 and waste strength；(2) chlorella carries out domestication culture in waste water, it is final obtain in volume fraction is 50% 70% waste water can fast-growth algae kind；(3) the algae kind seed liquor after domestication is inoculated into waste water, cultivated in closed photo bioreactor；(4) chlorella is harvested, wastewater treatment is completed.The method of the present invention is used purifies pig farm fermentation waste water in the stronger chlorella of adaptability, can preferably remove TN, TP and COD of waste water, solves current pig farm fermentation waste water using conventional method is not economical enough or the problem of direct exhaust emission environment；In addition, the chlorella of harvest can be used as other purposes, certain economic benefit is produced.

Description

A kind of method that utilization chlorella purifies pig farm fermentation waste water
Technical field
The present invention relates to a kind of method that utilization chlorella purifies pig farm fermentation waste water.
Background technology
With expanding economy, the management mode trend scale of pig industry, development is swift and violent, and wastewater discharge also increasingly increases Plus, thing followed environmental problem is also increasingly severe.It is at current Intensive Farm of Pig Raising that piggery wastewater, which carries out fermentation methane production, Manage the biogas slurry after the more universal mode of waste water, but fermentation and still fall within high concentrated organic wastewater, it is impossible to reach discharge standard.Hair Biogas slurry treatment mode after ferment has artificial swamp method, biological pond method and MBR reactor for treatment etc., but these methods are due to cost mistake High the problems such as, is difficult to apply in practice, therefore the principal mode of pig farm fermentation waste water processing at present is after simple processing Directly discharge.
Chlorella is a kind of spherical unicellular fresh water algae, is a kind of efficient photosynthetic plant, is grown with photoautotrophy numerous Grow, intracellular protein, fat and carbohydrate content are all very high, there is multivitamin again, edible and be used as bait.
The content of the invention
The present invention proposes a kind of method that utilization chlorella purifies pig farm fermentation waste water, solves current pig farm fermentation useless The problem of water is using conventional method not enough economy or direct exhaust emission environment.
The technical proposal of the invention is realized in this way：
A kind of method that utilization chlorella purifies pig farm fermentation waste water, at least comprises the following steps：
(1) enter regulating reservoir after the pretreatment of pig farm fermentation waste water and adjust pH=6.5-7.5 and waste strength；
(2) chlorella carries out domestication culture in waste water, final to obtain the energy in volume fraction is 50%-70% waste water The algae kind of fast-growth；
(3) the algae kind seed liquor after domestication is inoculated into waste water, cultivated in closed photo bioreactor；
(4) chlorella is harvested, wastewater treatment is completed.
Preferably, preprocessing process is in step (1)：Pig farm fermentation waste water is after separation of solid and liquid, and clear liquid enters aeration tank exposure Gas, is aerated the laggard sedimentation basin sedimentation time 2h of 48h, and supernatant enters sterilization pool, sterilized with strong chlorine oil, and 30- is aerated after 16-24h 60min, adds sodium thiosulfate and neutralizes.
Preferably, the addition of the strong chlorine oil is 6-30g/m3, and sodium thiosulfate addition is the 2-3 of strong chlorine oil quality Times.
Preferably, chlorella is tamed in volume fraction is 20%-100% waste water successively in step (2), chlorella is put Cultivated on shaking table in incubated room, 23-26 DEG C of cultivation temperature, shaking speed 180rmp/min, illumination 3000- 4000lux, the algae kind of fast-growth can be capable of in volume fraction is 50%-70% waste water by finally sifting out.
Preferably, step (3) is specially：Seed liquor that is good and spreading cultivation will be tamed to be inoculated into pretreated waste water, Cultivated in Closed photobioreactor, inoculum concentration volume fraction is 10%-20%, makes OD=0.2-0.3, condition of culture is pH =6.5-7.5, illumination 5000-6000lux, 23-26 DEG C of temperature are cultivated 9-12 days.
Preferably, flocculant used in harvesting chlorella is that ferric trichloride is final concentration of in ferric trichloride, algae solution in step (4) 200-500mg/L, pH=6-7.
What the present invention was produced has the beneficial effect that：Pig farm fermentation waste water, energy are purified using in the stronger chlorella of adaptability TN, TP and COD of waste water are preferably removed, current pig farm fermentation waste water is solved using conventional method not enough economy or directly arranges The problem of putting pollution environment；In addition, the chlorella of harvest can be used as other purposes, certain economic benefit is produced.
Embodiment
Technical scheme is clearly and completely described below in conjunction with the embodiment of the present invention, it is clear that retouched The embodiment stated is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, sheet The every other embodiment that field those of ordinary skill is obtained under the premise of creative work is not made, belongs to the present invention The scope of protection.
Embodiment
(1) fermentation waste water clear liquid after separation of solid and liquid in pig farm enters aeration tank aeration, the laggard sedimentation basins of 48h is aerated, during precipitation Between 2h, supernatant liquor enters sterilization pool, uses 15g/m3Strong chlorine oil is sterilized, and 30min is aerated after 16h, adds 2.5 times of quality of strong chlorine oil Sodium thiosulfate is neutralized, and is subsequently entered regulating reservoir regulation PH and is 7 and adjusts waste strength with running water.
(2) the pig farm fermentation waste water after above-mentioned processing is successively the dense of 20-100% in volume fraction since low concentration Chlorella is tamed in the waste water of degree, chlorella is placed on the shaking table in incubated room and cultivated, 23-26 DEG C of cultivation temperature, shaking table Rotating speed 180rmp/min, illumination 3000-4000lux, finally sift out can concentration be 50%-70% pig farms fermentation waste water in can The algae kind of fast-growth.
(3) algae kind seed liquor that is good and spreading cultivation will be tamed to be inoculated into pretreated waste water, choose 50%, 60%, 70% 3 concentration, is cultivated in Closed photobioreactor respectively, and inoculum concentration volume fraction is the 10%-20% of waste water, Make OD=0.2-0.3, condition of culture is PH=7, illumination 5000-6000lux, and 25 DEG C of temperature is cultivated 12 days.
(4) after culture a cycle, chlorella is harvested, flocculant used is ferric trichloride, and ferric trichloride is dense eventually in algae solution Spend for 200-500mg/L, PH=6, after 2h, the sedimentation rate of chlorella is up to more than 95%, and supernatant liquor discharge, which is collected, completes useless Water process；Harvest after chlorella, take the supernatant after flocculation to survey the index values such as COD, TN, TP.
Pig farm fermentation waste water finds that 60% waste water energy reaches best clean-up effect after above-mentioned steps are handled, and As shown in table 1, final each contamination index can reach livestock breeding wastewater discharge standard to result after processing.
Table 1：Pig farm fermentation waste water contamination index before and after the processing
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.

Claims (6)

1. a kind of method that utilization chlorella purifies pig farm fermentation waste water, it is characterised in that at least comprise the following steps：
(1) enter regulating reservoir after the pretreatment of pig farm fermentation waste water and adjust pH=6.5-7.5 and waste strength；
(2) chlorella carries out domestication culture in waste water, and final obtain can be quick in volume fraction is 50%-70% waste water The algae kind of growth；
(3) the algae kind seed liquor after domestication is inoculated into waste water, cultivated in closed photo bioreactor；
(4) chlorella is harvested, wastewater treatment is completed.
2. the method that a kind of utilization chlorella as claimed in claim 1 purifies pig farm fermentation waste water, it is characterised in that step (1) preprocessing process is in：Pig farm fermentation waste water is after separation of solid and liquid, and clear liquid enters aeration tank aeration, is aerated the laggard sedimentation basins of 48h Sedimentation time 2h, supernatant enters sterilization pool, is sterilized with strong chlorine oil, and 30-60min is aerated after 16-24h, adds sodium thiosulfate and neutralizes.
3. the method that a kind of utilization chlorella as claimed in claim 2 purifies pig farm fermentation waste water, it is characterised in that described strong The addition of chlorine essence is 6-30g/m3, and sodium thiosulfate addition is 2-3 times of strong chlorine oil quality.
4. the method that a kind of utilization chlorella as claimed in claim 1 purifies pig farm fermentation waste water, it is characterised in that step (2) chlorella is tamed in volume fraction is 20%-100% waste water successively in, chlorella is placed in shaking in incubated room Cultivated on bed, 23-26 DEG C of cultivation temperature, shaking speed 180rmp/min, illumination 3000-4000lux, finally sifting out can be in volume Fraction is is capable of the algae kind of fast-growth in 50%-70% waste water.
5. the method that a kind of utilization chlorella as claimed in claim 1 purifies pig farm fermentation waste water, it is characterised in that step (3) it is specially：Seed liquor that is good and spreading cultivation will be tamed to be inoculated into pretreated waste water, in Closed photobioreactor Culture, inoculum concentration volume fraction is 10%-20%, makes OD=0.2-0.3, condition of culture is pH=6.5-7.5, illumination 5000- 6000lux, 23-26 DEG C of temperature is cultivated 9-12 days.
6. the method that a kind of utilization chlorella as claimed in claim 1 purifies pig farm fermentation waste water, it is characterised in that step (4) flocculant used in harvesting chlorella is ferric trichloride final concentration of 200-500mg/L, pH=6- in ferric trichloride, algae solution in 7。
CN201710139296.9A 2017-03-09 2017-03-09 A kind of method that utilization chlorella purifies pig farm fermentation waste water Pending CN107055946A (en)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107629961A (en) * 2017-10-16 2018-01-26 韶关学院 A kind of method using breeding wastewater culture microalgae
CN107686815A (en) * 2017-10-17 2018-02-13 中国水产科学研究院渔业机械仪器研究所 A kind of method that chlorella is cultivated using food garbage
CN107827328A (en) * 2017-12-14 2018-03-23 陈英 A kind of technique for repairing breeding wastewater
CN108865892A (en) * 2018-05-29 2018-11-23 深圳文科园林股份有限公司 A method of utilizing livestock breeding biogas slurry high yield microalgae
CN109502914A (en) * 2018-12-24 2019-03-22 江西洁地环境治理生态科技有限公司 A kind of sewage disposal system using chlorella processing biogas slurry
CN110894467A (en) * 2019-11-12 2020-03-20 湖南工业大学 Method for culturing chlorella by using starch processing wastewater anaerobic fermentation liquid
CN111635080A (en) * 2020-06-30 2020-09-08 福州科力恩生物科技有限公司 Sewage treatment system and application thereof in purification of breeding sewage and black and odorous water
CN112551700A (en) * 2020-11-25 2021-03-26 武汉市农业科学院 Method for purifying biogas slurry by using microalgae
CN113045133A (en) * 2021-03-24 2021-06-29 重庆大学 System and method for treating livestock and poultry breeding wastewater by anaerobic fermentation coupled with microalgae organisms
CN113717854A (en) * 2021-09-01 2021-11-30 东北农业大学 Oil-producing chlorella ZM-5 and application thereof
CN114525209A (en) * 2022-02-17 2022-05-24 日照职业技术学院 Method for treating organic wastewater by using microalgae

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CN103396950A (en) * 2013-08-09 2013-11-20 烟台大学 Biogas slurry ecological purification method based on microalgae cultivation

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CN103396950A (en) * 2013-08-09 2013-11-20 烟台大学 Biogas slurry ecological purification method based on microalgae cultivation

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107629961A (en) * 2017-10-16 2018-01-26 韶关学院 A kind of method using breeding wastewater culture microalgae
CN107686815A (en) * 2017-10-17 2018-02-13 中国水产科学研究院渔业机械仪器研究所 A kind of method that chlorella is cultivated using food garbage
CN107827328A (en) * 2017-12-14 2018-03-23 陈英 A kind of technique for repairing breeding wastewater
CN108865892A (en) * 2018-05-29 2018-11-23 深圳文科园林股份有限公司 A method of utilizing livestock breeding biogas slurry high yield microalgae
CN109502914B (en) * 2018-12-24 2023-10-31 江西洁地环境治理生态科技有限公司 Sewage treatment system for treating biogas slurry by using chlorella
CN109502914A (en) * 2018-12-24 2019-03-22 江西洁地环境治理生态科技有限公司 A kind of sewage disposal system using chlorella processing biogas slurry
CN110894467A (en) * 2019-11-12 2020-03-20 湖南工业大学 Method for culturing chlorella by using starch processing wastewater anaerobic fermentation liquid
CN111635080A (en) * 2020-06-30 2020-09-08 福州科力恩生物科技有限公司 Sewage treatment system and application thereof in purification of breeding sewage and black and odorous water
CN112551700A (en) * 2020-11-25 2021-03-26 武汉市农业科学院 Method for purifying biogas slurry by using microalgae
CN112551700B (en) * 2020-11-25 2024-02-13 武汉市农业科学院 Method for purifying biogas slurry by utilizing microalgae
CN113045133A (en) * 2021-03-24 2021-06-29 重庆大学 System and method for treating livestock and poultry breeding wastewater by anaerobic fermentation coupled with microalgae organisms
CN113717854A (en) * 2021-09-01 2021-11-30 东北农业大学 Oil-producing chlorella ZM-5 and application thereof
CN113717854B (en) * 2021-09-01 2023-05-02 东北农业大学 Chlorella oleaginous ZM-5 and application thereof
CN114525209A (en) * 2022-02-17 2022-05-24 日照职业技术学院 Method for treating organic wastewater by using microalgae

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Application publication date: 20170818