CN102618446A - Method for cultivating oleaginous microalgae by using fecal sewage - Google Patents
Method for cultivating oleaginous microalgae by using fecal sewage Download PDFInfo
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- CN102618446A CN102618446A CN2012101106112A CN201210110611A CN102618446A CN 102618446 A CN102618446 A CN 102618446A CN 2012101106112 A CN2012101106112 A CN 2012101106112A CN 201210110611 A CN201210110611 A CN 201210110611A CN 102618446 A CN102618446 A CN 102618446A
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Abstract
The invention relates to a method for cultivating oleaginous microalgae by using fecal sewage, which comprises the steps of pretreatment of the fecal sewage, inoculation activation and acclimated cultivation of microalgae, amplification cultivation of the microalgae, accumulation of lipid content of the microalgae, concentration of the microalgae and collection of the microalgae. According to the method, a liquid which is discharged during the process of feces recycling treatment and conforms to the emission standard is used for cultivating the microalgae so that cultivation water and reagents are saved during the microalgae cultivation, simultaneously, the microalgae can be used without being sterilized, the production process is reduced, the cost of obtaining microalgae biodiesel is reduced, nitrogen and phosphorus in the liquid serve as nutrient materials during the microalgae cultivation, various indexes in the sewage are further reduced, and water quality of the sewage is improved. Besides, biogas produced during the process of feces recycling treatment serve as a heating fuel, carbon dioxide (CO2) separated during biogas purification serve as a carbon source for the microalgae cultivation, the microalgae cultivation and the feces recycling treatment are organically coupled, and energy conservation and emission reduction are promoted while the cost is reduced.
Description
Technical field
The present invention relates to WWT and environmental technology field, particularly a kind of method of utilizing pretreated fecal sewage to cultivate the little algae of produce oil.
Background technology
At present, Greenhouse effect and energy shortage have been the key difficult problems that the whole world faces, and alleviate Greenhouse effect and solve the Sustainable development that energy dilemma is directly connected to global economy.Biofuel has green property and recyclability; Be considered to solve one of the most desirable approach of global energy crisis, yet at present because the oil crops of preparation biofuel mostly are food crop, for example: corn, soybean and vegetable seed etc.; Striving grain and striving the ground phenomenon with the people will appear in such production of biodiesel mode; Sustainability is relatively poor, and production cost is higher, the industry development difficulty.Little algae is not owing to occupy the arable land, and oleaginousness is high, and in produce oil, has the effect of carbon capture, and day by day being construed to is the potential raw material of production biofuel.Extensive little algal biomass resource acquisition difficulty and little algae bioenergy product cost are too high to be the two big bottlenecks that present little algae bioenergy technology faces.Carry out industrialization with present technology, have the problem that the large scale culturing floor space is excessive, infrastructure investment is too high, the material consumption of course of processing energy consumption is excessive.To above problem, the research tendency of present little algae biofuel mainly concentrates on productive rate that improves little algae and the cost that reduces little algae cultivation.
Aspect the little algae cultivation of reduction cost, research mainly concentrates on foundation and development utilization waste water and fires the waste gas CO of factory
2Carry out the technology of little algae ecologic breeding, the thing that reduces in little algae culturing process can consume, and alleviates the region restriction that little algae is cultivated, fundamentally to solve the cost bottleneck of cultivating occupation of land, water consumption.There is research to hope through utilizing the resource culturing microalgae of the carbonic acid gas that produces in trade effluent and the industrial production, the preparation biofuel.But the pollutent that often contains higher concentration owing to the moity complicacy of trade effluent suppresses micro algae growth.Also there is method (CN 101955846A) to carry out microalgae grease production, but in little algae culturing process, pollutes, need sterilize, expend the energy based on sewage low-carbon emission resource utilization.
Summary of the invention
The present invention is directed to above technical background, provide a kind of fecal sewage to cultivate the method for the little algae of produce oil.This method is to utilize in the fecal sewage treating processes to filter out the raw material of water culturing microalgae as the production biofuel through membrane bioreactor, further removes nitrogen, phosphorus in the water simultaneously, can realize reducing the cost of culturing microalgae and the double effects of wastewater treatment.
The present invention adopts the cultured continuously mode; Collect the raw material of the high little algae of acquisition total lipid content as the production biofuel through fecal sewage processing, the inoculation of little algae and preparatory cultivation, enlarged culturing and after accumulating little algae lipid content, technical scheme of the present invention realizes through following steps:
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, it comprises:
(1) pre-treatment of fecal sewage: the qualified discharge liquid that obtains after solid-liquid separation, flocculation dewatering, aerobic compost, anaerobic treatment, the oxygen processing of holding concurrently, aerobic treatment, membrane biological reaction processing and stink are administered; This liquid does not contain mikrobe, and suspended substance is less than 3mg/L, pH6-9; Ammonia-nitrogen content 30-35mg/L; Total phosphorous 1-6mg/L, directly as the nutrient solution of little algae, it is subsequent use to handle the collecting methane that obtains for it;
Wherein: it is further comprising the steps of:
(2) little algae expansion is planted and acclimation shaking culture: in sterilisable chamber, 5 of the algae kind accesses of the little algae of produce oil are equipped with in the Erlenmeyer flask of 2L F/2 nutrient solution, shake up, the initial density of little algae algae kind is (200-300) * 10
4Individual cell/mL; With little algae algae kind be seeded in the F/2 nutrient solution in; Little algae and F/2 nutrient solution volume ratio are 1: (4-6), at 22-26 ℃, illumination is 14h: 10h with the dark time cycle; Expand kind of a cultivation under the condition of intensity of illumination 1800-2000lux, every at a distance from 6-8 hour CO of feeding in each Erlenmeyer flask
2Gas, CO
2Ventilation Rate 2-5L/min, each aeration time is 3 minutes, the little algae number in Erlenmeyer flask reaches (20-100) * 10
4During individual cell/mL, obtained expanding and kind cultivated a back algae liquid; Expansion kind in above-mentioned 5 Erlenmeyer flasks cultivated in No. 1 bioreactor that back algae liquid is transferred to the nutrient solution that the little algae of step (1) is housed carry out acclimation shaking culture; The volume ratio that expands the nutrient solution of kind cultivating little algae of being equipped with in back algae liquid and No. 1 bioreactor is 1: 1-1: 4; The volume of No. 1 bioreactor is 50L; The acclimation shaking culture temperature is 22-26 ℃; Illumination is 14h: 10h with the dark time cycle, in intensity of illumination is to carry out acclimation shaking culture under the condition of 1800-2000lux, whenever in bioreactor, feeds a CO at a distance from 6-8 hour
2Gas, CO
2Ventilation Rate 3-6L/min, each aeration time is 5 minutes; The algae kind adapts to nutrient solution after acclimation shaking culture 1-2 days, begins the breeding growth, and the nutrient solution in the breeding growth is called algae liquid, as the absorbancy (OD of algae liquid
680) carry out step (3a) when reaching 0.5-0.8;
(3a) little algae enlarged culturing: the algae liquid in the step (2) is put into bioreactor No. 2; The volume of No. 2 bioreactors is 50L; Culture temperature remains 22-26 ℃; Illumination is 12h: 12h with the dark time cycle, and intensity of illumination is 2500-4000lux, and is every at a distance from 6-8 hour CO of feeding in bioreactor
2Gas, CO
2Ventilation Rate 3-6L/min, each aeration time is 5 minutes, cultivates after 36-48 hour, as the absorbancy (OD of algae liquid
680) entering step (4) when reaching 1.8-2;
(3b) little algae enlarged culturing: the algae liquid that will from step (4), return is put into bioreactor No. 2; Simultaneously the nutrient solution of little algae in the step (1) is joined in No. 2 bioreactors; The amount that adds the nutrient solution of little algae in the step (1) is that the volume of No. 2 bioreactors is long-pending for deducting the algae liquid that from step (4), returns; The volume 50L of No. 2 bioreactors, culture temperature remains 22-26 ℃, and illumination is 12h: 12h with the dark time cycle; Intensity of illumination is 2500-4000lux, and is every at a distance from 6-8 hour CO of feeding in bioreactor
2Gas, CO
2Ventilation Rate 3-6L/min, each aeration time is 5 minutes, cultivates after 36-48 hour, as algae liquid absorbancy (OD
680) entering step (4) when reaching 1.8-2;
(4) accumulation of little algae lipid content: will be from step (3a) or the algae liquid of sending here (3b) put into bioreactor No. 3; The volume of No. 3 bioreactors is 50L; Illumination is 16h: 8h with the dark time cycle, and intensity of illumination is adjusted into 6000-8000lux, CO
2Ventilation Rate is reduced to 1-3L/min, in bioreactor, send one time CO in 3-4 hour
2, each aeration time is 5 minutes, cultivates after 3-5 days, as the absorbancy (OD of algae liquid
680) when reaching 2.5-3.2, the algae liquid of 97.5%-98% carries out the concentrating of little algae of step (5); The algae liquid of 2%-2.5% returns step (3b) and carries out little algae enlarged culturing;
(5) concentrating of little algae: the algae liquid that will from No. 3 bioreactors, send here is sent in the membrane reactor through transferpump, and membrane reactor inside is equipped with ultra-filtration membrane, and CO is equipped with in the ultra-filtration membrane below
2The ventpipe of gas, the liquid in the algae liquid is got into by the aperture on ultra-filtration membrane surface, and being compiled by the ultra-filtration membrane center becomes the clear water discharge; The algae liquid that does not discharge is trapped within outside the membrane bioreactor; Become liquid concentrator, when ultrafiltration membrance filter, to membrane bioreactor, charge into CO from ventpipe
2Gas is used CO
2The bur on ultra-filtration membrane surface, CO are fallen in gas clean-up
2The feeding amount of gas is 100-200Nm with the ratio of ultra-filtration membrane shadow area
3/ m
2/ h; Liquid concentrator separated into two parts in the membrane bioreactor; The liquid concentrator that wherein accounts for the 10%-20% of liquid concentrator volume ratio returns in No. 3 bioreactors of step (4) and proceeds to cultivate, and improving the absorbancy of little algae, the 80-90% of liquid concentrator volume ratio send step (6);
(6) collection of little algae: the liquid concentrator that will from step (5), send here is sent in the whizzer, under 6000 rev/mins condition, carries out centrifugally, and liquid is by the whizzer of dishing out; Little algae is stayed in the whizzer; Little algae of staying on the whizzer is taken out collection, and dried 12 hours down at 80-110 ℃, obtaining proportion is little algae of 0.4-0.75g/L; The total lipid content of little algae accounts for the 20-30% of little algae gross weight
Above-mentioned CO
2Gas is CO
2With Air mixing gas, wherein CO
2Volume content be the 6-8% (V/V) of total mixed gas;
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, wherein: with CO that discharge, that have little time to absorb in step (2), step (3a), step (3b), step (4) and the step (5)
2Gas is used the 10%NaOH solution absorption;
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, wherein: the aperture of the interior ultra-filtration membrane of membrane reactor is 0.1um in the step (5);
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, wherein: said absorbancy (OD
680) be the absorbancy of under 680 nano wave lengths, measuring;
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, wherein: the NaHCO that contains 12000mg in every liter of F/2 substratum in the step (2)
3, 20mg KH
2PO
4, 100mg KNO
3, 30mg NH
2CONH
2, 20mg MgSO
47H
2The NaCl of O, 1000mg, the ZnSO of 0.023mg
44H
2The MnCl of O, 0.078mg
24H
2The CuSO of O, 0.01mg
45H
2The FeC of O, 0.0039mg
6H
5O
75H
2The Na of O, 0.0073mg
2MoO
42H
2The CoCl of O, 0.012mg
26H
2The Na of O, 0.00435mg
2The vitamins B of EDTA, 0.5mg
12Vitamins B with 100mg
1
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, wherein: in the biogas of step (1), contain the carbonic acid gas of 20-30% and the methane of 60-70%, after above-mentioned biogas is sent into the molecular sieve dehydration jar and carried out deep dehydration; Send into knockout tower and carry out carbon dioxide separation; In knockout tower, utilize NHD solution that carbonic acid gas is adsorbed, discharge methane, the pressure of knockout tower is 1.5-5.5MPa; Temperature is 1-15 ℃; The NHD solution that has adsorbed carbonic acid gas is sent in the flash drum, and the pressure of flash drum is 0.08MPa, because pressure drop; Carbonic acid gas just comes out from the NHD solution flash; The high-pureness carbon dioxide that flash distillation is come out is packed into through pressure maintaining valve in the carbon dioxide storage tank, supplies to use in step (2) to the step (5), and the methane that discharges is sent in the step (6) as the fuel of the little algae of heating, drying or sent in the power generation system and generate electricity.
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, wherein: the CO described in step (2), step (3a), step (3b), step (4) and the step (5)
2CO in the gas
2It is the high-pureness carbon dioxide in the carbon dioxide storage tank of from the biogas of step (1), separating of packing into;
A kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention, wherein: said algae kind is available from hydrobiont institute of Chinese Academy of Sciences algae kind storehouse.
Beneficial effect of the present invention is embodied in:
1. utilize the membrane biological reaction water outlet culturing microalgae in the dung recovery treating processes, practiced thrift and cultivated water and reagent in little algae culturing process.Need not sterilization simultaneously can use, and shortens production technique, reduces the cost that obtains little algae biofuel.
2. the nutritive substance when little algae utilizes the nitrogen phosphorus in the membrane biological reaction water outlet to cultivate as little algae further reduces each item index in the sewage, the water quality of purifying waste water.
3. utilize the biogas that produces in the dung recovery treating processes to separate the CO2 that obtains as fuel of heat supply, when biogas is purified and cultivate carbon source, little algae is cultivated with dung recovery handled organic coupling, promote energy-saving and emission-reduction when reducing cost as little algae.
Description of drawings
Fig. 1 cultivates the method block diagram of the little algae of produce oil for the present invention utilizes fecal sewage.
Embodiment
As shown in the figure, a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil of the present invention comprises:
(1) pre-treatment of fecal sewage:
The purpose of this step is fecal sewage is handled; Make it become the nutrient solution that is applicable to micro algae growth, it is that " method that ight soil focuses on ", the patent No. are the method described in the ZL 200910092443.7 in the denomination of invention of application on September 8th, 2009 that application artificial " Beijing CanFit Resource Technologies Inc. " is adopted in the pre-treatment of fecal sewage.The qualified discharge liquid that ight soil and sewage obtain after administering through solid-liquid separation, flocculation dewatering, aerobic compost, anaerobic treatment, the oxygen processing of holding concurrently, aerobic treatment, membrane biological reaction processing and stink; Though this liquid has reached emission standard; But, can not reuse because it is nitrogenous all higher with amount phosphorus.This liquid does not contain mikrobe, and suspended substance (SS) is less than 3mg/L, pH6-9, and ammonia-nitrogen content 30-35mg/L, total phosphorous 1-6mg/L meets the micro algae growth condition, and directly as the nutrient solution of little algae, the collecting methane that in above-mentioned anaerobic treatment process, produces is subsequent use for it;
(2) little algae expansion is planted and acclimation shaking culture:
The purpose of this step is to enlarge and tame little algae, makes the nutrient solution of little algae adaptation step (1), after buying bacterial classification; Because bacterial classification has been in active state, but because the bacterial classification of buying is less, and not too adapt to the nutrient solution of little algae; Therefore need expand and plant and acclimation shaking culture; When bacterial classification increases to when a certain amount of, just can use the bacterial classification that expands kind and inoculate and culture out directly to carry out the enlarged culturing of little algae, and no longer need this step.
In sterilisable chamber, the algae kind of the little algae of produce oil (Botryococcus braunii) that will buy from hydrobiont institute of Chinese Academy of Sciences algae kind storehouse inserts 5 Erlenmeyer flasks that 2L F/2 nutrient solution is housed, and wherein contains the NaHCO of 12000mg in every liter of F/2 substratum
3, 20mg KH
2PO
4, 100mg KNO
3, 30mg NH
2CONH
2, 20mg MgSO
47H
2The NaCl of O, 1000mg, the ZnSO of 0.023mg
44H
2The MnCl of O, 0.078mg
24H
2The CuSO of O, 0.01mg
45H
2The FeC of O, 0.0039mg
6H
5O
75H
2The Na of O, 0.0073mg
2MoO
42H
2The CoCl of O, 0.012mg
26H
2The Na of O, 0.00435mg
2The vitamins B of EDTA, 0.5mg
12Vitamins B with 100mg
1, shaking up, the initial density of little algae algae kind is (200-300) * 10
4Individual cell/mL; With little algae algae kind be seeded in the F/2 nutrient solution in; Little algae and F/2 nutrient solution volume ratio are 1: (4-6), at 22-26 ℃, illumination is 14h: 10h with the dark time cycle; Expand kind of a cultivation under the condition of intensity of illumination 1800-2000lux, every at a distance from 6-8 hour CO of feeding in each Erlenmeyer flask
2Gas, CO
2Ventilation Rate 2-5L/min, each aeration time is 3 minutes, the little algae number in Erlenmeyer flask reaches (20-100) * 10
4During individual cell/mL, obtained expanding and kind cultivated a back algae liquid; Expansion kind in above-mentioned 5 Erlenmeyer flasks cultivated in No. 1 bioreactor that back algae liquid is transferred to the nutrient solution that the little algae of step (1) is housed carry out acclimation shaking culture; The volume ratio that expands the nutrient solution of kind cultivating little algae of being equipped with in back algae liquid and No. 1 bioreactor is 1: 1-1: 4; The volume of No. 1 bioreactor is 50L; The acclimation shaking culture temperature is 22-26 ℃; Illumination is 14h: 10h with the dark time cycle, in intensity of illumination is to carry out acclimation shaking culture under the condition of 1800-2000lux, whenever in bioreactor, feeds a CO at a distance from 6-8 hour
2Gas, CO
2Ventilation Rate 3-6L/min, each aeration time is 5 minutes; The algae kind adapts to nutrient solution after acclimation shaking culture 1-2 days, begins the breeding growth, and the nutrient solution in the breeding growth is called algae liquid, as the absorbancy (OD of algae liquid
680) carry out step (3a) when reaching 0.5-0.8, on address the absorbancy (OD of the following stated
680) be the absorbancy of under 680 nano wave lengths, measuring.
(3a) little algae enlarged culturing:
Algae liquid in the step (2) is put into bioreactor No. 2; The volume of No. 2 bioreactors is 50L, and culture temperature remains 22-26 ℃, and illumination is 12h: 12h with the dark time cycle; Intensity of illumination is 2500-4000lux, and is every at a distance from 6-8 hour CO of feeding in bioreactor
2Gas, CO
2Ventilation Rate 3-6L/min, each aeration time is 5 minutes, cultivates after 36-48 hour, as the absorbancy (OD of algae liquid
680) entering step (4) when reaching 1.8-2;
(3b) little algae enlarged culturing:
The algae liquid that will from step (4), return is put into bioreactor No. 2; Simultaneously the nutrient solution of little algae in the step (1) is joined in No. 2 bioreactors; The amount that adds the nutrient solution of little algae in the step (1) is that the volume of No. 2 bioreactors is long-pending for deducting the algae liquid that from step (4), returns; The volume 50L of No. 2 bioreactors, culture temperature remains 22-26 ℃, and illumination is 12h: 12h with the dark time cycle; Intensity of illumination is 2500-4000lux, and is every at a distance from 6-8 hour CO of feeding in bioreactor
2Gas, CO
2Ventilation Rate 3-6L/min, each aeration time is 5 minutes, cultivates after 36-48 hour, as the absorbancy (OD of algae liquid
680) entering step (4) when reaching 1.8-2;
The purpose of step (3a) and the little algae enlarged culturing of step (3b) is to accumulate the micro algae biomass of logarithmic phase, removes nitrogen, phosphorus nutrition material in the nutrient solution.
(4) accumulation of little algae lipid content:
Through step (3a) or cultivation (3b); The concentration of nitrogen ammonia is reduced to 5-10mg/l in the nutrient solution, and the phenomenal growth of microalgae cell density is owing to cell density and the growth velocity of little algae can reduce along with the minimizing of nitrogen phosphoric concentration; Therefore under this condition, little algae begins synthetic fatty acid.This step purpose is: when improving little algae lipid content, promote the growth that it is biological, adopt to prolong light application time and intensity of illumination that it has solved because little algae density increases each other light being blocked of being produced, and causes the problem that growth velocity descends.
From step (3a) or the algae liquid of sending here (3b) put into bioreactor No. 3, the volume of No. 3 bioreactors is 50L, illumination is 16h: 8h with the dark time cycle, intensity of illumination is adjusted into 6000-8000lux, CO
2Ventilation Rate is reduced to 1-3L/min, in bioreactor, send one time CO in 3-4 hour
2, each aeration time is 5 minutes, cultivates after 3-5 days, as the absorbancy (D of algae liquid
680) when reaching 2.5-3.2, the algae liquid of 97.5%-98% carries out the concentrating of little algae of step (5); The algae liquid of 2%-2.5% returns step (3b) and carries out little algae enlarged culturing;
(5) concentrating of little algae: the nutrient solution that will from No. 3 bioreactors, send here and the mixed solution of little algae are sent in the membrane reactor through transferpump, and membrane reactor inside is equipped with ultra-filtration membrane, and the aperture of ultra-filtration membrane is 0.1um, and CO is equipped with in the ultra-filtration membrane below
2The ventpipe of gas; Liquid in the algae liquid is got into by the aperture on ultra-filtration membrane surface; Being compiled by the ultra-filtration membrane center becomes clear water and discharges, and nitrogen, phosphorus content in the clear water of this discharge further reduce, and have reached GB/T 18920-2002 " city miscellaneous water water quality standard "; This water can carry out second stage employ, as flushes the toilet, waters flowers and plants and flushing road etc.The nutrient solution that does not discharge and little algae are trapped within the membrane bioreactor, become liquid concentrator, when ultrafiltration membrance filter, charge into CO from ventpipe toward membrane bioreactor
2Gas is used CO
2The bur on ultra-filtration membrane surface is fallen in gas clean-up, prevents dirty blocking up, and influences the filtration velocity of ultra-filtration membrane.CO
2The feeding amount of gas is 100-200Nm with the ratio of ultra-filtration membrane shadow area
3/ m
2/ h.Liquid concentrator separated into two parts in the membrane bioreactor; The liquid concentrator that wherein accounts for the 10%-20% of liquid concentrator volume ratio returns in No. 3 bioreactors of step (4) and proceeds to cultivate; Improving the absorbancy of little algae, the 80-90% of liquid concentrator volume ratio send step (6);
(6) collection of little algae: the liquid concentrator that will from step (5), send here is sent in the whizzer; Under 6000 rev/mins condition, carry out centrifugally, liquid is by the whizzer of dishing out, and little algae is stayed in the whizzer; Little algae of staying on the whizzer is taken out collection; And under 80-110 ℃, dried 12 hours, obtaining proportion is little algae of 0.4-0.75g/L, the total lipid content of little algae accounts for the 20-30% of little algae gross weight; , resulting little algae utilizes processes biofuel through oil refining process.
CO that discharge, that have little time to absorb in step (2), step (3a), step (3b), step (4) and step (5)
2Gas is used the 10%NaOH solution absorption, reclaims post-treatment as the industrial chemicals of producing yellow soda ash.
In the biogas of step (1), contain the carbonic acid gas of 20-30% and the methane of 60-70%, after above-mentioned biogas is sent into the molecular sieve dehydration jar and carried out deep dehydration, send into knockout tower and carry out carbon dioxide separation; In knockout tower, utilize NHD solution that carbonic acid gas is adsorbed, discharge methane, the pressure of knockout tower is 1.5-5.5MPa; Temperature is 1-15 ℃; The NHD solution that has adsorbed carbonic acid gas is sent in the flash drum, and the pressure of flash drum is 0.08MPa, because pressure drop; Carbonic acid gas just comes out from the NHD solution flash; The high-pureness carbon dioxide that flash distillation is come out is packed into through pressure maintaining valve in the carbon dioxide storage tank, supplies to use in step (2) to the step (5), and the methane that discharges is sent in the step (6) as the fuel of the little algae of heating, drying or sent in the power generation system and generate electricity.
Step (2) is to the CO described in the step (5)
2Gas is high-pureness carbon dioxide and the Air mixing gas in the carbon dioxide storage tank of from the biogas of step (1), separating of packing into, and the volume content of carbonic acid gas is the 6-8% (V/V) of total mixed gas in the mixed gas.
Above embodiment is not that the scope that the present invention limited is referring to claim to the restriction of invention just to explanation of the present invention, and under the situation of spirit of the present invention, the present invention can make any type of modification.
Claims (8)
1. method of utilizing fecal sewage to cultivate the little algae of produce oil, it comprises: the pre-treatment of (1) fecal sewage: solid-liquid separation, flocculation dewatering, aerobic compost, anaerobic treatment, the oxygen processing of holding concurrently, aerobic treatment, the qualified discharge liquid that membrane biological reaction is handled and stink obtains after administering, this liquid does not contain mikrobe; Suspended substance is less than 3mg/L; PH6-9, ammonia-nitrogen content 30-35mg/L, total phosphorous 1-6mg/L; Directly as the nutrient solution of little algae, it is subsequent use to handle the collecting methane that obtains for it;
Wherein: it is further comprising the steps of:
(2) little algae expansion is planted and acclimation shaking culture: in sterilisable chamber, 5 of the algae kind accesses of the little algae of produce oil are equipped with in the Erlenmeyer flask of 2L F/2 nutrient solution, shake up, the initial density of little algae algae kind is (200-300) * 10
4Individual cell/mL; With little algae algae kind be seeded in the F/2 nutrient solution in; Little algae and F/2 nutrient solution volume ratio are 1: (4-6), at 22-26 ℃, illumination is 14h:10h with the dark time cycle; Expand kind of a cultivation under the condition of intensity of illumination 1800-2000 lux, every at a distance from 6-8 hour CO of feeding in each Erlenmeyer flask
2Gas, CO
2Ventilation Rate 2-5 L/min, each aeration time is 3 minutes, the little algae number in Erlenmeyer flask reaches (20-100) * 10
4During individual cell/mL, obtained expanding and kind cultivated a back algae liquid; Expansion kind in above-mentioned 5 Erlenmeyer flasks cultivated in No. 1 bioreactor that back algae liquid is transferred to the nutrient solution that the little algae of step (1) is housed carry out acclimation shaking culture; The volume ratio that expands the nutrient solution of kind cultivating little algae of being equipped with in back algae liquid and No. 1 bioreactor is 1:1-1:4; The volume of No. 1 bioreactor is 50L; The acclimation shaking culture temperature is 22-26 ℃; Illumination is 14h:10h with the dark time cycle, in intensity of illumination is to carry out acclimation shaking culture under the condition of 1800-2000 lux, whenever in bioreactor, feeds a CO at a distance from 6-8 hour
2Gas, CO
2Ventilation Rate 3-6 L/min, each aeration time is 5 minutes; The algae kind adapts to nutrient solution after acclimation shaking culture 1-2 days, begins the breeding growth, and the nutrient solution in the breeding growth is called algae liquid, as the absorbancy (OD of algae liquid
680) carry out step (3a) when reaching 0.5-0.8;
(3a) little algae enlarged culturing: the algae liquid in the step (2) is put into bioreactor No. 2; The volume of No. 2 bioreactors is 50L; Culture temperature remains 22-26 ℃; Illumination is 12h:12h with the dark time cycle, and intensity of illumination is 2500-4000lux, and is every at a distance from 6-8 hour CO of feeding in bioreactor
2Gas, CO
2Ventilation Rate 3-6 L/min, each aeration time is 5 minutes, cultivates after 36-48 hour, as the absorbancy (OD of algae liquid
680) entering step (4) when reaching 1.8-2;
(3b) little algae enlarged culturing: the algae liquid that will from step (4), return is put into bioreactor No. 2; Simultaneously the nutrient solution of little algae in the step (1) is joined in No. 2 bioreactors; The amount that adds the nutrient solution of little algae in the step (1) is that the volume of No. 2 bioreactors is long-pending for deducting the algae liquid that from step (4), returns; The volume 50L of No. 2 bioreactors, culture temperature remains 22-26 ℃, and illumination is 12h:12h with the dark time cycle; Intensity of illumination is 2500-4000lux, and is every at a distance from 6-8 hour CO of feeding in bioreactor
2Gas, CO
2Ventilation Rate 3-6 L/min, each aeration time is 5 minutes, cultivates after 36-48 hour, as algae liquid absorbancy (OD
680) entering step (4) when reaching 1.8-2;
(4) accumulation of little algae lipid content: will be from step (3a) or the algae liquid of sending here (3b) put into bioreactor No. 3; The volume of No. 3 bioreactors is 50L; Illumination is 16h:8h with the dark time cycle, and intensity of illumination is adjusted into 6000-8000lux, CO
2Ventilation Rate is reduced to 1-3 L/min, in bioreactor, send one time CO in 3-4 hour
2, each aeration time is 5 minutes, cultivates after 3-5 days, as the absorbancy (OD of algae liquid
680) when reaching 2.5-3.2, the algae liquid of 97.5%-98% carries out the concentrating of little algae of step (5); The algae liquid of 2%-2.5& returns step (3b) and carries out little algae enlarged culturing;
(5) concentrating of little algae: the algae liquid that will from No. 3 bioreactors, send here is sent in the membrane reactor through transferpump, and membrane reactor inside is equipped with ultra-filtration membrane, and CO is equipped with in the ultra-filtration membrane below
2The ventpipe of gas, the liquid in the algae liquid is got into by the aperture on ultra-filtration membrane surface, and being compiled by the ultra-filtration membrane center becomes the clear water discharge; The algae liquid that does not discharge is trapped within outside the membrane bioreactor; Become liquid concentrator, when ultrafiltration membrance filter, to membrane bioreactor, charge into CO from ventpipe
2Gas is used CO
2The bur on ultra-filtration membrane surface, CO are fallen in gas clean-up
2The feeding amount of gas is 100-200Nm with the ratio of ultra-filtration membrane shadow area
3/ m
2/ h; Liquid concentrator separated into two parts in the membrane bioreactor; 10%-20% the liquid concentrator that wherein accounts for liquid concentrator volume ratio returns in No. 3 bioreactors of step (4) and proceeds to cultivate, improving the absorbancy of little algae, liquid concentrator volume ratio 80-90% send step (6);
(6) collection of little algae: the liquid concentrator that will from step (5), send here is sent in the whizzer; Under 6000 rev/mins condition, carry out centrifugally, liquid is by the whizzer of dishing out, and little algae is stayed in the whizzer; Little algae of staying on the whizzer is taken out collection; And under 80-110 ℃, dried 12 hours, obtaining proportion is little algae of 0.4-0.75 g/L, the total lipid content of little algae accounts for the 20-30% of little algae gross weight;
Above-mentioned CO
2Gas is CO
2With Air mixing gas, wherein CO
2Volume content be the 6-8% (V/V) of total mixed gas.
2. a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil as claimed in claim 1 is characterized in that: with CO that discharge, that have little time to absorb in step (2), step (3a), step (3b), step (4) and the step (5)
2Gas is used the 10%NaOH solution absorption.
3. a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil as claimed in claim 1 is characterized in that: the aperture of the interior ultra-filtration membrane of membrane reactor is 0.1um in the step (5).
4. a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil as claimed in claim 1 is characterized in that: said absorbancy (OD
680) be the absorbancy of under 680 nano wave lengths, measuring.
5. a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil as claimed in claim 1 is characterized in that: the NaHCO that contains 12000mg in every liter of F/2 substratum in the step (2)
3, 20mg KH
2PO
4, 100mg KNO
3, 30mg NH
2CONH
2, 20mg MgSO
47H
2The NaCl of O, 1000mg, the ZnSO of 0.023mg
44H
2The MnCl of O, 0.078mg
24H
2The CuSO of O, 0.01mg
45H
2The FeC of O, 0.0039mg
6H
5O
75H
2The Na of O, 0.0073mg
2MoO
42H
2The CoCl of O, 0.012mg
26H
2The Na of O, 0.00435mg
2The vitamins B of EDTA, 0.5mg
12Vitamins B with 100mg
1
6. a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil as claimed in claim 1 is characterized in that: in the biogas of step (1), contain 20-30% carbonic acid gas and 60-70% methane, after above-mentioned biogas is sent into the molecular sieve dehydration jar and carried out deep dehydration; Send into knockout tower and carry out carbon dioxide separation; In knockout tower, utilize NHD solution that carbonic acid gas is adsorbed, discharge methane, the pressure of knockout tower is 1.5-5.5MPa; Temperature is 1-15 ℃; The NHD solution that has adsorbed carbonic acid gas is sent in the flash drum, and the pressure of flash drum is 0.08MPa, because pressure drop; Carbonic acid gas just comes out from the NHD solution flash; The high-pureness carbon dioxide that flash distillation is come out is packed into through pressure maintaining valve in the carbon dioxide storage tank, supplies to use in step (2) to the step (5), and the methane that discharges is sent in the step (6) as the fuel of the little algae of heating, drying or sent in the power generation system and generate electricity.
7. a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil as claimed in claim 6, it is characterized in that: said step (2) is to the CO described in the step (5)
2CO in the gas
2It is the high-pureness carbon dioxide in the carbon dioxide storage tank of from the biogas of step (1), separating of packing into.
8. a kind of method of utilizing fecal sewage to cultivate the little algae of produce oil as claimed in claim 1, it is characterized in that: said algae kind is available from hydrobiont institute of Chinese Academy of Sciences algae kind storehouse.
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