CN105524872A - Application ascorbic acid in improving microalgae biomass - Google Patents
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Abstract
The invention provides application of ascorbic acid or pharmaceutically acceptable salts, esters, hydrates or glucoside derivatives in improving microalgae biomass. The invention also provides a microalgae culture method. Researches show that the ascorbic acid can improve the biomass of the microalgae and can promote wastewater recycling; therefore, the ascorbic acid has a good application prospect.
Description
Technical field
The invention belongs to field of environment protection and field of renewable energy, relate to the novelty teabag of xitix, particularly xitix improves the purposes of micro algae biomass.
Background technology
Energy dilemma and water pollution are two hang-ups of current Faced In Sustainable Development.Wherein, biofuel has renewable, easy degraded, the post-combustion pollution thing discharge feature such as low and be considered to desirable fungible energy source, but faces the predicament of raw material sources deficiency.Prepare biofuel with micro-algae for raw material and there is the advantage that fat content is high, growth cycle is short, do not occupy cultivated land, but the density of microdisk electrode is lower at present, toxigenic capacity is too high.
On the other hand, along with our expanding economy, the organic waste water of the materials such as a large amount of rich Nitrogen-and Phosphorus-containings is unprocessed to be just exhausted in PE, causes serious water environment pollution.The denitrogenation dephosphorizing of waste water is a great problem of current wastewater treatment, and processing cost is higher, and can cause the waste of the resources such as nitrogen phosphorus.
Utilize organic waste water to cultivate micro-algae, both can reduce the cost of microdisk electrode, the object of utilization of wastewater resource can be reached again, receive the concern of more and more investigator.But many growths being unfavorable for micro-algae containing ammonia nitrogen in high density etc. in municipal wastewater, food processing wastewater and breeding wastewater at present.The most frequently used method of current raising waste water cultivating microalgae biomass is diluted waste water with fresh water, and the method needs to consume a large amount of Freshwater resources, and actual application value is lower, is unfavorable for the development of microdisk electrode industry.
Xitix (L-Ascorbicacid), has another name called vitamins C, is a kind of water-soluble vitamins, within 1907, is found in lemon juice by Norway's chemist's Halkett, within 1934, obtains sterling, now can synthetic.Xitix has the biological activity such as metabolism, prevention cardiovascular diseases promoting osso-albumin biosynthesizing, promote tyrosine and tryptophane in amino acid.Have no the report that xitix is relevant to micro-algae at present, more have no the report that xitix improves micro algae biomass in organic waste water.
Summary of the invention
Xitix or its pharmacy acceptable salt, ester, hydrate, glycosides derivatives is the object of the present invention is to provide to improve the purposes of micro algae biomass and a kind of microalgae culture method.
The invention provides xitix or its pharmacy acceptable salt, purposes that ester, hydrate, glycosides derivatives improve micro algae biomass.
Micro algae biomass: refer to the weight of micro-algae in unit volume.
Wherein, described micro-algae is Chlorella (Chlorella), Scenedesmus (Scenedesmus), Spirullina (Phaeodactylum), plan Chlorella (Parachlorella), salt algae (Dunaliella), Phaeodactylum tricornutum (Phaeodactylumtricornutum) or Botryococcus braunii (Botryococcusbraunii).
Present invention also offers a kind of microalgae culture method, step is as follows:
Get micro-algae, being inoculated in ammonia-nitrogen content is in the micro algae culturing liquid of 10-1000mg/L, adds xitix or its pharmacy acceptable salt, ester, hydrate, glycosides derivatives, cultivates 5-14 days.
Micro algae culturing liquid: refer to the liquid that can maintain micro algae growth breeding, comprises the substratum that various waste water such as municipal wastewater, sanitary sewage, food processing wastewater, breeding wastewater comprise natural pond liquid etc. and artificial preparation.
Ammonia-nitrogen content is the micro algae culturing liquid of 10-1000mg/L: refer to that the weight of the ammonia-state nitrogen in every 1L micro algae culturing liquid is 10-1000mg.
Wherein, described micro-algae is Chlorella, Scenedesmus, Spirullina, plan Chlorella, salt algae, Phaeodactylum tricornutum or Botryococcus braunii.
Wherein, often liter of micro algae culturing liquid, inoculates micro-algae 0.1-2g.
Micro-algae of inoculation refers to that the centrifugal micro-algae obtained, centrifugal condition can be 4000rpm, 2-5min, and micro-algae 0.1-2g is micro-algae weight in wet base 0.1-2g through activation is to logarithmic growth after date in activation medium.
Wherein, described micro algae culturing liquid is municipal wastewater, sanitary sewage, food processing wastewater, breeding wastewater.
Further, described breeding wastewater is natural pond liquid.
The necessary material of the micro algae growths such as natural pond liquid is the fermented liquid that biogas fermentation material generates through anaerobically fermenting, its rich Nitrogen-and Phosphorus-containing institute, and ammonia nitrogen equal size is high, belongs to high concentrated organic wastewater.Mostly fermentation raw material is feces of livestock and poultry.
Wherein, described natural pond liquid has carried out solid-liquid separation before the micro-algae of inoculation, collects liquid portion;
The method of described solid-liquid separation for leaving standstill, flocculation, to filter and/or centrifugal.
Wherein, in microdisk electrode process, the concentration of xitix in micro algae culturing liquid maintains 50-300mg/L.
Wherein, the method maintaining ascorbic acid concentrations is: after inoculating micro-algae, add xitix first, again add xitix afterwards at interval of 2-5 days;
Wherein, the xitix consumption added first adds xitix 50-150mg for often liter of nutrient solution, and the xitix consumption later at every turn added adds xitix 150-300mg for often liter of nutrient solution.
The most frequently used method of current raising waste water cultivating microalgae biomass is diluted waste water with fresh water, to reduce the organic concentration in waste water.The present invention surprisingly finds under study for action, and xitix can significantly improve the biomass of micro-algae, the micro algae biomass in high-concentration ammonia nitrogenous wastewater can be made to improve 3.1-6.1 doubly, achieve completely unexpected technique effect.
On the other hand, xitix of the present invention can make organic waste water turn waste into wealth, and improves the utility value of organic waste water, is conducive to the protection of ecotope; And the inventive method is simple, convenient operation, has a good application prospect.
Obviously, according to foregoing of the present invention, according to ordinary technical knowledge and the customary means of this area, not departing under the present invention's above-mentioned basic fundamental thought prerequisite, the amendment of other various ways, replacement or change can also be made.
With embodiment, content of the present invention is described further below, but the present invention is not limited to these embodiments.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment
The reagent that the present invention is used and instrument are commercially available.
All micro-algaes are all bought from Chinese Academy of Sciences's algae kind storehouse.
Micro-algae activates after buying, and activation step is as follows: under aseptic condition, is inoculated in respective substratum respectively by each micro-algae, and after 25 DEG C of cultivation 7d, centrifugal (4000rpm, 2-5min), collects thalline, weighs thalline weight in wet base, for subsequent use;
Wherein, chlorella and grid algae are BG11 substratum, and Botryococcus braunii is SE substratum.
Embodiment 1 microalgae culture method of the present invention
At natural pond, pig farm, Jianyang of Sichuan liquid (after measured, ammonia-nitrogen content is 200-350mg/L) in, inoculation chlorella, often liter of natural pond liquid inoculation 0.1g chlorella, adding final concentration is after xitix cultivation 4d (4 days) of 150mg/L, add the xitix continuation cultivation that final concentration is 200mg/L, then add every 2d the xitix continuation cultivation that final concentration is 200mg/L; Amount to after cultivating 10d, collect frond.
Embodiment 2 microalgae culture method of the present invention
In natural pond, pig farm, Jianyang of Sichuan liquid (after measured, ammonia-nitrogen content is 200-350mg/L), get the centrifugal 5min of natural pond liquid 4000rpm, get liquid portion as micro algae culturing liquid.
In micro algae culturing liquid, inoculation chlorella, often liter of micro algae culturing liquid inoculation 2g chlorella, adds the xitix cultivation 5d that final concentration is 100mg/L, add the xitix continuation cultivation that final concentration is 250mg/L, then add every 2d the xitix continuation cultivation that final concentration is 250mg/L; Amount to after cultivating 10d, collect frond.
Embodiment 3 microalgae culture method of the present invention
At natural pond, pig farm, Jianyang of Sichuan liquid (after measured, ammonia-nitrogen content is 10-200mg/L) in, inoculation Phaeodactylum tricornutum, often liter of natural pond liquid inoculation 1g Phaeodactylum tricornutum, add the xitix cultivation 3d that final concentration is 150mg/L, add every 3d the xitix continuation cultivation that final concentration is 250mg/L; Amount to after cultivating 7d, collect frond.
Embodiment 4 microalgae culture method of the present invention
At natural pond, pig farm, Jianyang of Sichuan liquid (after measured, ammonia-nitrogen content is 10-200mg/L) in, inoculation Botryococcus braunii, often liter of natural pond liquid inoculation 2g Botryococcus braunii, add the xitix cultivation 2d that final concentration is 150mg/L, then add every 2d the xitix continuation cultivation that final concentration is 250mg/L; Amount to after cultivating 14d, collect frond.
Embodiment 5 microalgae culture method of the present invention
In natural pond, pig farm, Qionglai, Sichuan liquid (after measured, ammonia-nitrogen content is 300-350mg/L), inoculation grid algae, often liter of natural pond liquid inoculation 0.1g grid algae, adds the xitix cultivation 12d that final concentration is 300mg/L, collects frond.
Embodiment 6 microalgae culture method of the present invention
In natural pond, pig farm, Qionglai, Sichuan liquid (after measured, ammonia-nitrogen content is 300-350mg/L), get the centrifugal 10min of natural pond liquid 3000rpm, get liquid portion as micro algae culturing liquid.
In micro algae culturing liquid, inoculation grid algae, often liter of micro algae culturing liquid inoculation 0.1g grid algae, adds the xitix that final concentration is 125mg/L, cultivates 4d, then add the xitix continuation cultivation of 150mg/L every 2d; Cultivate 12d altogether, collect frond.
Embodiment 7 microalgae culture method of the present invention
In natural pond, pig farm, Qionglai, Sichuan liquid (after measured, ammonia-nitrogen content is 400-500mg/L), get the centrifugal 8min of natural pond liquid 3000rpm, get liquid portion Nacl and regulate salinity to be 40, as micro algae culturing liquid.
In micro algae culturing liquid, inoculation salt algae, often liter of micro algae culturing liquid inoculation 0.1g salt algae, add the xitix that final concentration is 100mg/L, cultivate 3d, add the xitix continuation cultivation that final concentration is 150mg/L, then add the xitix continuation cultivation of 150mg/L every 3d; Cultivate 10d altogether, collect frond.
Embodiment 8 microalgae culture method of the present invention
In natural pond, pig farm, Qionglai, Sichuan liquid (after measured, ammonia-nitrogen content is 700-1000mg/L), get the centrifugal 5min of natural pond liquid 4000rpm, get liquid portion sodium bicarbonate adjust ph more than 8.0, as micro algae culturing liquid.
In micro algae culturing liquid, inoculation spirulina, often liter of micro algae culturing liquid inoculation 0.1g spirulina, add the xitix that final concentration is 150mg/L, cultivate 5d, add the xitix continuation cultivation that final concentration is 150mg/L, then add the xitix continuation cultivation of 300mg/L every 5d; Cultivate 14d altogether, collect frond.
With concrete testing data, beneficial effect of the present invention is described below.
The effect of test example 1 xitix in microdisk electrode
One, experimental technique
Get natural pond, pig farm, Jianyang of Sichuan liquid (after measured, ammonia-nitrogen content is 298mg/L), centrifugal, get liquid portion as micro algae culturing liquid.
Micro algae culturing liquid is equally divided into two parts, every part of 1L, wherein, a in micro algae culturing liquid, inoculation chlorella, often liter of micro algae culturing liquid inoculation 2g chlorella, directly cultivate 10d, collect chlorella, dry in the baking oven of 60 DEG C, weigh to measure chlorella biomass.
Portion in addition, in micro algae culturing liquid, inoculation chlorella, often liter of micro algae culturing liquid inoculation 2g chlorella, adds the xitix of 150mg/L, cultivates 4d, then adds the xitix continuation cultivation of 200mg/L every 2d; Cultivate 10d altogether, collect chlorella, dry in the baking oven of 60 DEG C, weigh to measure chlorella biomass.
Two, experimental result
In table 1.
The chlorella biomass contrast of table 1 different treatment
| Different treatment | Chlorella biomass (g/L) |
| Do not add xitix | 0.33 |
| Add xitix | 1.02 |
From table 1, do not add in the liquid of Vitamin C acid-treated natural pond, chlorella biomass is 0.33g/L, and add in the liquid of Vitamin C acid-treated natural pond, chlorella biomass is 1.02g/L, and does not add compared with xitix contrasts, and chlorella biomass (dry weight) improves 3.1 times.
The effect of test example 2 xitix in microdisk electrode
One, experimental technique
Get natural pond, pig farm, Qionglai, Sichuan liquid (after measured, ammonia-nitrogen content is 355mg/L), centrifugal, get liquid portion as micro algae culturing liquid.
Micro algae culturing liquid is equally divided into two parts, every part of 1L, wherein, a in micro algae culturing liquid, inoculation grid algae, often liter of micro algae culturing liquid inoculation 0.4g grid algae, directly cultivates 12d, collector mess algae, dries, weighs to measure grid algae biomass in the baking oven of 60 DEG C.
Portion in addition, in micro algae culturing liquid, inoculation grid algae, often liter of micro algae culturing liquid inoculation 0.4g grid algae, adds the xitix of 150mg/L, cultivates 4d, then add the xitix continuation cultivation of 150mg/L every 2d; Cultivate 12d altogether, collector mess algae, dry in the baking oven of 60 DEG C, weigh to measure grid algae biomass.
Two, experimental result
In table 2.
The grid algae biomass contrast of table 2 different treatment
| Different treatment | Grid algae biomass (g/L) |
| Do not add xitix | 0.28 |
| Add xitix | 1.71 |
From table 2, do not add in the liquid of Vitamin C acid-treated natural pond, grid algae biomass is 0.28g/L, and add in the liquid of Vitamin C acid-treated natural pond, chlorella biomass is 1.71g/L, and does not add compared with xitix contrasts, and chlorella biomass (dry weight) improves 6.1 times.
To sum up, xitix can improve the biomass of micro-algae greatly, it is in the micro algae culturing liquid of 10-1000mg/L that xitix or its pharmacy acceptable salt, ester, hydrate, glycosides derivatives are joined ammonia-nitrogen content, not only significantly improves the biomass of micro-algae, reduces the production cost of micro-algae; And improve the utility value of waste water, be conducive to the protection of ecotope, have a good application prospect.
Claims (10)
1. xitix or its pharmacy acceptable salt, ester, hydrate, glycosides derivatives improve the purposes of micro algae biomass.
2. purposes according to claim 1, is characterized in that, described micro-algae is Chlorella, Scenedesmus, Spirullina, plan Chlorella, salt algae, Phaeodactylum tricornutum or Botryococcus braunii.
3. a microalgae culture method, is characterized in that: step is as follows:
Get micro-algae, being inoculated in ammonia-nitrogen content is in the micro algae culturing liquid of 10-1000mg/L, adds xitix or its pharmacy acceptable salt, ester, hydrate, glycosides derivatives, cultivates 5-14 days.
4. method according to claim 3, is characterized in that: described micro-algae is Chlorella, Scenedesmus, Spirullina, plan Chlorella, salt algae, Phaeodactylum tricornutum or Botryococcus braunii.
5. method according to claim 3, is characterized in that: often liter of micro algae culturing liquid inoculates micro-algae 0.1-2g.
6. method according to claim 3, is characterized in that: described micro algae culturing liquid is municipal wastewater, sanitary sewage, food processing wastewater, breeding wastewater.
7. method according to claim 6, is characterized in that: described breeding wastewater is natural pond liquid.
8. method according to claim 3, is characterized in that: described natural pond liquid has carried out solid-liquid separation before the micro-algae of inoculation, collects liquid portion;
The method of described solid-liquid separation for leaving standstill, flocculation, to filter and/or centrifugal.
9. method according to claim 3, is characterized in that: in microdisk electrode process, and the concentration of xitix in micro algae culturing liquid maintains 50-300mg/L.
10. method according to claim 9, is characterized in that: the method maintaining ascorbic acid concentrations is: after inoculating micro-algae, add xitix first, again add xitix afterwards at interval of 2-5 days;
Wherein, the xitix consumption added first adds xitix 50-150mg for often liter of nutrient solution, and the xitix consumption later at every turn added adds xitix 150-300mg for often liter of nutrient solution.
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| CN201610070888.5A CN105524872A (en) | 2016-01-29 | 2016-01-29 | Application ascorbic acid in improving microalgae biomass |
| PCT/CN2016/103009 WO2017128781A1 (en) | 2016-01-29 | 2016-10-24 | Use of ascorbic acid in increasing mircroalgae biomass |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106318620A (en) * | 2016-08-15 | 2017-01-11 | 中国石油大学(华东) | Preparation and application of microalgae natural dye |
| WO2017128781A1 (en) * | 2016-01-29 | 2017-08-03 | 农业部沼气科学研究所 | Use of ascorbic acid in increasing mircroalgae biomass |
| CN114561295A (en) * | 2022-03-14 | 2022-05-31 | 福建农林大学 | Culture method for promoting accumulation and lipid synthesis of microalgae fucoxanthin |
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| CN112126597B (en) * | 2020-08-29 | 2022-08-12 | 东台市赐百年生物工程有限公司 | Method for culturing spirulina based on biogas slurry |
| CN112028251A (en) * | 2020-09-03 | 2020-12-04 | 上饶师范学院 | Method for improving ammonia nitrogen removal effect of microalgae on livestock and poultry breeding wastewater |
| CN111944735B (en) * | 2020-09-03 | 2023-07-04 | 上饶师范学院 | Method for promoting microalgae to grow in livestock and poultry breeding wastewater |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017128781A1 (en) * | 2016-01-29 | 2017-08-03 | 农业部沼气科学研究所 | Use of ascorbic acid in increasing mircroalgae biomass |
| CN106318620A (en) * | 2016-08-15 | 2017-01-11 | 中国石油大学(华东) | Preparation and application of microalgae natural dye |
| CN114561295A (en) * | 2022-03-14 | 2022-05-31 | 福建农林大学 | Culture method for promoting accumulation and lipid synthesis of microalgae fucoxanthin |
| CN114561295B (en) * | 2022-03-14 | 2023-10-31 | 福建农林大学 | Culture method for promoting accumulation of microalgae fucoxanthin and synthesis of lipid |
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| WO2017128781A1 (en) | 2017-08-03 |
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