CN104017730A - Culture medium and culture method for culturing amphora ovalis by using wastewater in canning plant - Google Patents
Culture medium and culture method for culturing amphora ovalis by using wastewater in canning plant Download PDFInfo
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- CN104017730A CN104017730A CN201410280626.2A CN201410280626A CN104017730A CN 104017730 A CN104017730 A CN 104017730A CN 201410280626 A CN201410280626 A CN 201410280626A CN 104017730 A CN104017730 A CN 104017730A
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Abstract
The invention discloses a culture medium and a culture method for culturing amphora ovalis by using wastewater in a canning plant, and the culture medium and the culture method can be used for treating the wastewater in the canning plant with low cost, utilizing wastes and obtaining microalgae with important bait value. The amphora ovalis traditionally cultured by adopting a Liao's improved culture solution and an open runway pool is slow in growth, low in yield, easy to contaminate and low in benefit, whereas aerated culture with transparent 1.5cm<3> cylindrical food-grade plastic drums is capable of realizing low cost and quick growth, avoiding pollution contamination and is easy to operate; the wastewater in the canning plant, sodium silicate, ammonium ferric citrate, ammonium molybdate, potassium chromate, fish meal, sodium carboxymethyl cellulose, L-arginine, potassium dihydrogen phosphate, medical stone meal, 920-type plant growth substance, vitamin S-3 solution, M-F trace element solution, sodium thioglycollate and the like are added in the culture medium, namely organic fertilizers and inorganic fertilizers are used together, so that the nutrition is more comprehensive and balanced, the growth rate of the amphora ovalis is greatly improved, and the yield is improved by 420%.
Description
Technical field
The invention belongs to aquaculture field, relate in particular to a kind of substratum and cultural method that utilizes canning factory's waste water to cultivate oval two eyebrow algaes.
Background technology
The a large amount of waste water of the annual discharge of canning factory, wherein, containing organism such as the sugar of higher concentration, organic acid, pectin, acid, alkali, if not treated, directly enter river, river, lake, reservoir, can consume in a large number dissolved oxygens, cause water hypoxia, make fish and other hydrobiont dead.Along with developing rapidly of tinned pre-industry, waste water handling problem causes people's common concern day by day.
Mainly there are four large series products, fruit, vegetables, meat, aquatic products in canning factory.Waste water quality and water yield difference that four kinds of products are discharged are very large, mainly contain protein, fat, carbohydrate in waste water.In production process, discharge waste water and can divide three classes.The first kind is compared with cleaning water, as water coolant after the immersion of fruit and vegetable and rinse water and sterilization, wherein recoverable after part water sterilization.Equations of The Second Kind is the waste water of discharging in production process, after processing, could discharge.The 3rd class is high-concentration waste water, and as to boil water in advance in fruit can water chestnut, canned vegetables mushroom is to boil water in advance, and canned meat is to boil water in advance.The main program of fruit can complete processing has: clean, peeling, tinning, sealed cans and sterilization, equipment and utensil washing etc.Can processing has stronger seasonality, and wastewater flow rate vary within wide limits mainly comprises that pre-treatment washes Guo Shui, equipment cleaning water, ground flushing water, outer row's recirculated water etc.Data demonstration, fruit can factory mean displacement is 3-8m
3/ t raw material, main carbohydrate containing, carbohydrate and pectin, phosphorus content is higher, and nitrogen content is lower, and pH value 5-10, is easily degraded by microorganisms, and organism and concentration of suspension are high, and BOD5/CODcr ratio is high, and biodegradability is good, adopts traditionally following treatment process.
1, biological natural purification method
Biological natural purification method comprises stabilization pond and polluted water land treatment system, less investment, and working cost is low, is applicable to Small Urban and drought and water shortage area.Fruit can production seasonality is stronger, and employing stabilization pond preferably is processed, and be the busy season general spring and autumn, and the waste water of generation can be stored in stabilization pond, and process water quality in winter can be purified, and enters rivers next year again.Pea can waste water is after five months store after deliberation, and 95% BOD5 is degraded.Small-sized canning factory wastewater discharge is few, can consider to adopt stabilization pond and soil natural purification method to process, and has both saved initial cost and operational management expense, can fully utilize waste water again.Shortcoming is that floor space is large, and the treatment time is long, inefficiency, and treatment effect is subject to weather effect.
2, aerobic method
The aerobic treatment of waste water is in the situation that free oxygen is provided, and take aerobic microbiological treatment process as main, makes the treatment process of the stable degraded of organism.Zhang Hailin etc. adopt the light filter material of synthetic, utilize air concussion mode to design a kind of high-rate filter, i.e. Biosmedi filter tank, in Shanghai plum forests three are got profit food factory's sewage disposal test run more than a year, effect is better.The treatment process design flooding quantity of Biosmedi biological filter is 3500m
3/ d, filtrate solvent loading is 3.6kgBOD5/m
3d, water inlet BOD5 is 100mg/L, water outlet BOD5 is 30mg/L.Operating records shows that water outlet COD in two stage biological filter tank is all lower than 100mg/L, and mean value is in 50mg/L left and right, and clearance is all greater than 30%, is up to 70%.Shortcoming is that construction investment is large, and maintenance cost is high.
3, anaerobic process
Anaerobic biotreatment method be anaerobion under oxygen free condition, organism is converted into methane and carbon dioxide.Canned pear wastewater treatment test shows, through anaerobic digestion in 30 hours, organic removal rate reached 90%, increases basicity and adds ammonia salt or urea can reduce digestion time to Digestive system.Germany adopts anaerobic digester system to process fruit juice processing waste water, and whole treatment scheme is by coarse filtration system throw outs such as () place to go pericarp, sands, two Buffer Pools (regulating pH value and nitrogen, phosphorus etc.), a up flow anaerobic sludge blanket reactor (591m
3) and an Aerobic Pond (8.5m
3) form, in system, UASB reactor organic loading CODcr is 60kg/m
3d, CODcr clearance reaches 80-90%.But this method complex process, troublesome poeration.
4, anaerobic-aerobic combination process
Anaerobic-aerobic treatment process can give full play of anaerobion and bear high density and high load capacity advantage, can reclaim organic energy again, aerobic speck fast growth, and treatment effect is good.Shortcoming is troublesome poeration, and floor space is large, and infrastructure investment is large.
In order to solve the deficiency of current technology, I have invented a kind of oval two eyebrow algae methods of canning factory's waste water cultivation that utilize, can low-cost processes canning factory waste water, can obtain again the two eyebrow algaes of biological product-ellipse of important food value, turn waste into wealth, turning harm into good, can significantly reduce sewage treatment equipment investment and running expense, is the optimal selection of canning factory's wastewater treatment.
Oval double eyebrow algae spp Bacillariophyta, plumage line Diatomacae, order is met in bivalve, Yu Wen algae section, double eyebrow algae spp, cell zygomorphy, shell face lanceolar, two end trimming shapes, two shells meet prosperity, tool central authorities tubercle, chromatoplast sheet or band shape, the long 20-24 micron of cell, wide 15-18 micron.25 ℃ of growth optimum temperutures, optimum illumination intensity 5000Lux, optimum pH 7-8.5, optimal salinity 28-32.Oval two eyebrow algae is the important bait that the famous and precious aquatic animal fry such as Bao, sea cucumber and sea urchin is produced, and is the key factor that determines seedling percent and the strong degree of seedling physique.In sea cucumber artificial breeding, oval two eyebrow algaes affect sea cucumber larva and adhere to and metamorphosis, and the quality and quantity of algae and breading sea cucumber seedlings success or failure, output just have direct relation.
Oval two eyebrow algae adopts Liao Shi Media modified, i.e. KNO traditionally
360 milligrams, Na
2siO
320 milligrams, Na
2hPO
412H
2o40 milligram, 1000 milliliters of sterilization seawater are also used open rectangle raceway pond to cultivate, and growth is slow, yield poorly, investment is large, easily pollute, benefit is low, and I adopt 1.5 cubic metres of transparent cylindric food grade plastic bucket inflations to cultivate now, and cost is low, and growth is fast, is difficult for polluting, scrub conveniently, and easy handling, and also I have increased canning factory's waste water in substratum, water glass, ferric ammonium citrate, ammonium molybdate, potassiumchromate, fish meal, Xylo-Mucine, xanthan gum, propylene glycol alginate, sodium D-isoascorbate, gluten powder, Tutofusin tris, urea, oxyacetic acid, titanium solution, yeast soaks powder, Sodium desoxycholate, soil extract, ooze leach liquor, horsehit leach liquor, chicken manure leach liquor, L-arginine, potassium primary phosphate, medical stone powder, 920 plant growth substances, vitamin(e) S-3 solution, M-F trace element solution, Trypsin, calcium superphosphate, sorbyl alcohol, N.F,USP MANNITOL, the nutritive ingredients such as sodium thioglycolate, fertilizer and inorganic fertilizer mix use, and nutrition is more comprehensive, equilibrium, oval two eyebrow algae speeds of growth significantly improve, and culture cycle shortens, and output improves 420%.
Summary of the invention
In order to overcome the deficiency of current technology, the invention provides a kind of substratum that utilizes the oval two eyebrow algaes of canning factory's waste water cultivation, fill a prescription as follows: 90 milliliters of canning factory's waste water, 2 milligrams of water glass, 1 milligram of ferric ammonium citrate, 2 milligrams of ammonium molybdates, 1 milligram of potassiumchromate, 20 milligrams of fish meal, 1 milligram of Xylo-Mucine, 3 milligrams of xanthan gum, 1 milligram of propylene glycol alginate, 5 milligrams of sodium D-isoascorbate, 1.2 milligrams of gluten powders, 0.1 milligram of Tutofusin tris, 1 milligram, urea, 1 milliliter of oxyacetic acid, 1 milliliter of titanium solution, yeast soaks 10 milligrams, powder, 3 milligrams of Sodium desoxycholates, 1.5 milliliters of soil extracts, 1.2 milliliters of ooze leach liquors, 2.5 milliliters of horsehit leach liquors, 1.5 milliliters of chicken manure leach liquors, 1 milligram of L-arginine, 4 milligrams of potassium primary phosphates, 4 milligrams of medical stone powders, 920 plant growth substance 8 international unit, 1 milliliter of vitamin(e) S-3 solution, 1 milliliter of M-F trace element solution, 4 milligrams of Trypsins, 4 milligrams of calcium superphosphate, 6 milligrams of sorbyl alcohols, 3 milligrams, N.F,USP MANNITOL, 2 milligrams of sodium thioglycolates, 1000 milliliters, pure seawater after clorox sterilization,
The two eyebrow algae cultural methods of ellipse, concrete steps are as follows:
Culture vessel is cylindric food grade plastic bucket, 1.5 cubic metres of volumes, and high-density polyethylene material is made (market is on sale), colourless or white, there is good light transmission, in order to algae, carry out photosynthesis, bucket is integral type structure, and 6 millimeters of wall thickness, have very large intensity and soundness.One circular lid is arranged at bucket top, above has the silk of revolving, rotatable and seal.40 centimetres of bucket top cover diameters, lid is stamped 2 holes, and gas-filled valve is inserted in a hole, and vapor pipe is inserted in another hole, and gas-filled valve and vapor pipe are all Glass tubings, 8 millimeters of internal diameters.Reactor is washed, with distilled water rinse 3 times, pack 1000 liters of substratum claimed in claim 1 into afterwards, inflation mixes 5 minutes, selects the two eyebrow algaes inoculations of vigorous, the free of contamination ellipse of vitality, inoculum density 4 * 10
4cell/mL; On lid, insert two clean Glass tubings, one longer, 65 centimetres, 15 centimetres, lid is exposed on top, the logical charge tube in lower end is connected with gas exhausting stone, in order to inflation (effect of gas exhausting stone is to form very little bubble, avoids the wave of air pocket or rolling to smash frustule); Another root is shorter, and 30 centimetres, lid is exposed at two ends, in order to exhaust; Two same thicknesses of Glass tubing, 8 millimeters of internal diameters; With clean scotch tape, two Glass tubings are fixed; Long Glass tubing upper end materials pipe is connected with aerator pump, is filled with mixed gas, and mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%; Continuous charge, aeration quantity should not be too large, with frustule, can not float; Outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 5000-10000Lux; Cultivate first 5 days, a simple and easy canopy was built in bucket top, and upper placement curtain shuts out the light, and makes intensity of illumination maintain 1500-2800Lux, the light injury of avoiding sunburst to cause cell; Within the 6th day, remove curtain, stronger illumination is conducive to the growth and breeding of cell and synthesizing of high unsaturated fatty acid; Be cultured to the 7th day, stop inflation, take away lid, supplement 100 liters of fresh cultures, to make up the moisture evaporating, cover lid, inflation, continues to cultivate, and to the 9th day, oval two eyebrow algae density was increased to 250 * 10
4cell/mL, stops cultivating, and from the water tap of bottom, emits algae liquid, after culture vessel is washed, adds disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate;
Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 20～50 parts of pileworts, 10～20 parts of Japanese Honeysuckle Stems, 15～25 parts of eucalyptus leaveses, 5～10 parts of cape jasmines, 5～15 parts, Radix Glycyrrhizae, anticorrosion auxiliary material 1-3 part, disinfection of Chinese drug liquor manufacture method: by fresh pilewort, Japanese Honeysuckle Stem, eucalyptus leaves, Radix Glycyrrhizae, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 80 orders more standby, with table balance, take respectively 120 grams of the pileworts of drying and pulverizing, 60 grams of Japanese Honeysuckle Stems, 60 grams, eucalyptus leaves leaf, 30 grams, Radix Glycyrrhizae, 30 grams of mountain Cape jasmine fruits, 6 grams of anticorrosion auxiliary materials, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 32-36 hour, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute,
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute,
Preferably, to the described reactor of selecting, minute two sterilized phases after washing, are first used high pressure nitrogen to spray sterilization, and sterilization 2-3 minute is used high-pressure carbon dioxide to spray sterilization, and sterilization 2-3 minute, carries out algae culture again after sterilization.
Preferably, described anticorrosion auxiliary material is edible salt.
Preferably, described anticorrosion auxiliary material is the mixture that acetoacetic ester, shikimic acid, prussic acid are moored in pears acid, Buddhist nun, and blending ratio is weight ratio of mixture 1: 1: 1: 0.5.
Preferably, described anticorrosion auxiliary material component is for going out pears acid, Buddhist nun moors acetoacetic ester, shikimic acid, the mixture of prussic acid, blending ratio is weight ratio of mixture 1: 1: 1: 0.5, the preparation process of described anticorrosion auxiliary material is: with table balance, take out respectively pears acid, Buddhist nun moors acetoacetic ester, shikimic acid, prussic acid, blending ratio is weight ratio of mixture 1: 1: 1: 0.5, be placed in after the abundant mixing of Erlenmeyer flask, 85% ethanol that adds mixture 30-50 times volume, shake up and staticly settle 12-15 hour, skim liquid level floater, get clear liquid at the middle and upper levels, be anticorrosion auxiliary material, be sealed in black reagent bottle, labelled, be placed in 3-4 ℃ of refrigerator standby.
Useful result:
In order to overcome canning factory's waste water traditional treatment method shortcoming, I have invented a kind of substratum and cultural method that utilizes canning factory's waste water to cultivate oval two eyebrow algaes, not only can low-cost processes canning factory waste water, utilization of waste material, turn waste into wealth, and can obtain the two eyebrow algaes of ellipse of important food value, achieve many things at one stroke, really be the ingenious utmost point, simply fantastic.It is KNO that oval two eyebrow algae adopts Liao Shi Media modified traditionally
360 milligrams, Na
2siO
320 milligrams, Na
2hPO
412H
2o40 milligram, 1000 milliliters of sterilization seawater are also used open rectangle raceway pond to cultivate, and growth is slow, yield poorly, investment is large, easily pollute, benefit is low, and I adopt 1.5 cubic metres of transparent cylindric food grade PE plastic bucket inflations to cultivate now, and cost is low, and growth is fast, is difficult for polluting, scrub conveniently, and easy handling, and also I have increased canning factory's waste water in substratum, water glass, ferric ammonium citrate, ammonium molybdate, potassiumchromate, fish meal, Xylo-Mucine, xanthan gum, propylene glycol alginate, sodium D-isoascorbate, gluten powder, Tutofusin tris, urea, oxyacetic acid, titanium solution, yeast soaks powder, Sodium desoxycholate, soil extract, ooze leach liquor, horsehit leach liquor, chicken manure leach liquor, L-arginine, potassium primary phosphate, medical stone powder, 920 plant growth substances, vitamin(e) S-3 solution, M-F trace element solution, Trypsin, calcium superphosphate, sorbyl alcohol, N.F,USP MANNITOL, the nutritive ingredients such as sodium thioglycolate, fertilizer and inorganic fertilizer mix use, and nutrition is more comprehensive, equilibrium, oval two eyebrow algae speeds of growth significantly improve, and culture cycle shortens, and output improves 420%.
Description of drawings 1 is oval two eyebrow algae morphological structure figure;
Wherein Reference numeral is: 1 cell walls, 2 chromatoplasts, 3 central tubercles, 4 shell seams;
Fig. 2 is a kind of cultural method figure that utilizes canning factory's waste water to cultivate oval two eyebrow algaes;
Wherein Reference numeral is: 5 gas-filled valves, 6 plastic buckets, 7 algae liquid, 8 gas exhausting stones, 9 water taps, 10 vapor pipes.
Embodiment
Canning factory's pretreatment method for wastewater: canning factory's waste water needs to carry out pre-treatment before supporting algae, method is: first use 60 object silk cover filtering waste water, remove floating matter, glass bits, hair, silt, leaf, the larger solid substance of plastics bag equal-volume, after sewage is introduced in long 30 meters, wide 20 meters, the cement aeration tank of dark 0.7 meter, it is east-west that pond is, to accept more solar radiation, the depth of water remains on 60 centimetres, (unslaked lime preferably adds the amount of 5 liters of pure water to dissolve in plastic tank by 1 kilogram to unslaked lime in advance by every cubic metre of sewage, to add the amount of 50 grams of unslaked limes evenly to splash, dilution, stir, full pool spilling head again), every 60 minutes, (alum preferably dissolves in plastic tank by 100 grams of amounts that add 4 liters of pure water in advance by every cubic metre of sewage, to add the amount full pool spilling head alum of 80 grams again, dilute and stir, full pool spilling head again), then with rake, stir or air agitation 60 minutes, lime and alum are fully dissolved, mix and with sewage in organism fully there is chemical reaction, then natural sedimentation, fermentation, expose 72 hours, skim the floating matters such as water surface foam, at the bottom of not stirring pond, get sewage at the middle and upper levels, pack in lighttight black plastic bucket, sealing, 4 ℃ of refrigerators or freezer, preserve, standby,
Clorox sterilization seawater method: get seawater, absorbent cotton filters, and by adding the amount of 75 grams, chlorinated lime to add chlorinated lime in 1 cubic metre of seawater, makes available chlorine content in seawater reach 20mg/L, and inflation mixed after 12 hours, uses in Sulfothiorine and chlorine residue.Sulfothiorine amount is determined by the number of chlorine residue, until splash into the nondiscoloration of starch potassium iodide liquid;
Peracetic acid disinfectant liquid and preparation method thereof and application notice: the former liquid hold-up 35% of commercially available Peracetic Acid (by weight), colourless liquid, has intense stimulus smell.During use, apply clean tap water and be diluted to 0.2% solution, soak raceway pond inwall 2 hours.These product have intense stimulus effect to eyes, skin, mucous membrane and the upper respiratory tract, headache, nauseating and inflammable, and tool explosivity, severe corrosive, strong and stimulating, can cause human body and burn, and is heated to 100 ℃ of i.e. fierce decomposition, meets fiery or be heated, be subject to shake all can detonate.Contact with reductive agent, promotor, organism, combustiblematerials etc. vigorous reaction can occur; Not directly catch tactile, during operation, should be with rubber gloves, mouth mask and glasses, metal is corrosive, be not useable for the sterilization of metallic weapon; The long-term placement of stoste can be decomposed, and notes the quality guaranteed period, should store in cool dark place in plastic tank and preserve.
The two eyebrow algaes of ellipse of inoculation use are purified (this area ordinary method, as long as can realize purification, Kai Shi extraction method for example) in advance, and algae kind is in eugonic exponential phase of growth when guaranteeing to inoculate;
Various nutritive salt will add by the order providing in formula, to avoid chemical reaction;
Various inorganic nutrient salts and VITAMIN need be made in advance mother liquor and be placed in 4 ℃ of refrigerator cold-storages and save backup, and storage period is not over 45 days;
Chicken manure leach liquor preparation method: dried poultrymanure, green grass, soya-bean cake, rice bran, grain husk shell (shells of five cereals) and vegetable mould were by 60: 10: 12: the part by weight of 15: 2: 1 mixes, stir, pack airtight fermentation in cylinder into, after 30 days, pour out, at clean water mire, stand lamellar, dry 48 hours, after get 2 kilograms, be placed in plastic bucket, add 10 kilograms, tap water, stir, with 80 order silk cover filterings, clear liquid is boiled 5 minutes, cooling, standing 24 hours, get supernatant liquor, pack reagent bottle into, labelled, be placed in 4 ℃ of refrigerators, standby,
Soil Extract preparation method: get loose in the woods, be rich in 1 kilogram of organic upper layer of soil, add 5 liters, tap water, boil 20 minutes, mud is poured in beaker, standing 24 hours, rear absorption supernatant liquor, pour in Erlenmeyer flask, add cotton plug, boil 15 minutes, cooling, staticly settle 12 hours, get supernatant liquor and obtain soil Extract, pack grinding port plug reagent bottle into, sealing, labelled, be placed in 4 ℃ of refrigerators standby;
Medical stone powder becomes floury by medical stone raw ore through screening, grinding and processing, and granular size can be passed through 200 mesh sieve thin,tough silk;
920 plant growth substances are white crystalline powder, are difficult for being dissolved in water, can first with alcohol, fully dissolve (general every gram dissolves more than 1 hour with 50 ml ethanols or 60 degree white wine), then be watered dilution by desired concn;
Horsehit leach liquor preparation method: get 50 kilograms of fresh horsehits, add 3 kilograms, wheat bran, 1 kilogram of Semen Maydis powder, 4 liters, tap water, fully stir, pile coniform, spontaneous fermentation 15 days, rear stand lamellar, under sunlight, be exposed to the sun 3 days, after get 3 kilograms and put into bucket, add 10 liters of distilled water, fully stir evenly, standing, 100 order silk cover filterings after 48 hours, get filtrate heating, boil 45 minutes, kill bacterium wherein, mould, virus etc., cooling, precipitate 24 hours, get supernatant liquor, obtain horsehit leach liquor, pack grinding port plug reagent bottle into, sealing, be placed in 4 ℃ of refrigerators or freezer standby,
Ooze extracts liquid and preparation method thereof: get the more fertile black ooze of what not again, with 1 part of mud, add 2 parts of fresh water, stir, standing 1-2 minute, gets upper strata mud in aluminum pot, and the amount that adds 1 gram of NaOH by every 1000 milliliters of mud adds NaOH, boils 25 minutes.While boiling, need constantly stir, boil and within latter standing 24 hours, get supernatant liquor, sealing, is placed in 4 ℃ of refrigerators or freezer standby;
Titanium solution is joined method: 736.6 milligrams of titanous oxalates, be dissolved in 100 ml distilled waters, add 5 grams of ammonium hydroxide, make it alkalize, precipitate, by centrifuging, will precipitate and concentrate, with the sulfuric acid dissolution of 20 milliliters of 0.05mol/L and get final product;
M-F trace element solution is joined method: 0.2 gram of boric acid, and 0.2 gram of Manganous chloride tetrahydrate, 0.2 milligram of molybdic oxide, 0.02 gram, copper sulfate, 0.02 gram, zinc sulfate, 0.02 gram of cobalt chloride, is dissolved in 1000 ml distilled waters, finally splashes into a vitriol oil, shakes up and get final product;
Vitamin(e) S-3 solution is joined method: 50 milligrams of thiamine salt hydrochlorates, and 10 milligrams of Nicotinicum Acidums, 10 milligrams of calcium pantothenate, 1.0 grams of para-amino benzoic acid, 0.1 gram of vitamin H, 0.5 gram of inositol, folic acid 0.2 microgram, between chest, pyridine is 0.3 gram, 1000 milliliters of distilled water;
Pharmaceutical chemicals purity used all needs chemical pure rank;
A kind of substratum that utilizes the oval two eyebrow algaes of canning factory's waste water cultivation, fill a prescription as follows: 90 milliliters of canning factory's waste water, 2 milligrams of water glass, 1 milligram of ferric ammonium citrate, 2 milligrams of ammonium molybdates, 1 milligram of potassiumchromate, 20 milligrams of fish meal, 1 milligram of Xylo-Mucine, 3 milligrams of xanthan gum, 1 milligram of propylene glycol alginate, 5 milligrams of sodium D-isoascorbate, 1.2 milligrams of gluten powders, 0.1 milligram of Tutofusin tris, 1 milligram, urea, 1 milliliter of oxyacetic acid, 1 milliliter of titanium solution, yeast soaks 10 milligrams, powder, 3 milligrams of Sodium desoxycholates, 1.5 milliliters of soil extracts, 1.2 milliliters of ooze leach liquors, 2.5 milliliters of horsehit leach liquors, 1.5 milliliters of chicken manure leach liquors, 1 milligram of L-arginine, 4 milligrams of potassium primary phosphates, 4 milligrams of medical stone powders, 920 plant growth substance 8 international unit, 1 milliliter of vitamin(e) S-3 solution, 1 milliliter of M-F trace element solution, 4 milligrams of Trypsins, 4 milligrams of calcium superphosphate, 6 milligrams of sorbyl alcohols, 3 milligrams, N.F,USP MANNITOL, 2 milligrams of sodium thioglycolates, 1000 milliliters, pure seawater after clorox sterilization,
The two eyebrow algae cultural methods of ellipse, concrete steps are as follows:
Culture vessel is cylindric food grade plastic bucket, 1.5 cubic metres of volumes, and high-density polyethylene material is made (market is on sale), colourless or white, there is good light transmission, in order to algae, carry out photosynthesis, bucket is integral type structure, and 6 millimeters of wall thickness, have very large intensity and soundness.One circular lid is arranged at bucket top, above has the silk of revolving, rotatable and seal.40 centimetres of bucket top cover diameters, lid is stamped 2 holes, and gas-filled valve is inserted in a hole, and vapor pipe is inserted in another hole, and gas-filled valve and vapor pipe are all Glass tubings, 8 millimeters of internal diameters.Reactor is washed, with distilled water rinse 3 times, pack 1000 liters of substratum claimed in claim 1 into afterwards, inflation mixes 5 minutes, selects the two eyebrow algaes inoculations of vigorous, the free of contamination ellipse of vitality, inoculum density 4 * 10
4cell/mL; On lid, insert two clean Glass tubings, one longer, 65 centimetres, 15 centimetres, lid is exposed on top, the logical charge tube in lower end is connected with gas exhausting stone, in order to inflation (effect of gas exhausting stone is to form very little bubble, avoids the wave of air pocket or rolling to smash frustule); Another root is shorter, and 30 centimetres, lid is exposed at two ends, in order to exhaust; Two same thicknesses of Glass tubing, 8 millimeters of internal diameters; With clean scotch tape, two Glass tubings are fixed; Long Glass tubing upper end materials pipe is connected with aerator pump, is filled with mixed gas, and mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%; Continuous charge, aeration quantity should not be too large, with frustule, can not float; Outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 5000-10000Lux; Cultivate first 5 days, a simple and easy canopy was built in bucket top, and upper placement curtain shuts out the light, and makes intensity of illumination maintain 1500-2800Lux, the light injury of avoiding sunburst to cause cell; Within the 6th day, remove curtain, stronger illumination is conducive to the growth and breeding of cell and synthesizing of high unsaturated fatty acid; Be cultured to the 7th day, stop inflation, take away lid, supplement 100 liters of fresh cultures, to make up the moisture evaporating, cover lid, inflation, continues to cultivate, and to the 9th day, oval two eyebrow algae density was increased to 250 * 10
4cell/mL, stops cultivating, and from the water tap of bottom, emits algae liquid, after culture vessel is washed, adds disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate;
Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 20～50 parts of pileworts, 10～20 parts of Japanese Honeysuckle Stems, 15～25 parts of eucalyptus leaveses, 5～10 parts of cape jasmines, 5～15 parts, Radix Glycyrrhizae, anticorrosion auxiliary material 1-3 part, disinfection of Chinese drug liquor manufacture method: by fresh pilewort, Japanese Honeysuckle Stem, eucalyptus leaves, Radix Glycyrrhizae, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 80 orders more standby, with table balance, take respectively 120 grams of the pileworts of drying and pulverizing, 60 grams of Japanese Honeysuckle Stems, 60 grams, eucalyptus leaves leaf, 30 grams, Radix Glycyrrhizae, 30 grams of mountain Cape jasmine fruits, 6 grams of anticorrosion auxiliary materials, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 32-36 hour, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute,
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute,
Preferably, to the described reactor of selecting, minute two sterilized phases after washing, are first used high pressure nitrogen to spray sterilization, and sterilization 2-3 minute is used high-pressure carbon dioxide to spray sterilization, and sterilization 2-3 minute, carries out algae culture again after sterilization.
Preferably, described anticorrosion auxiliary material is edible salt.
Preferably, described anticorrosion auxiliary material is the mixture that acetoacetic ester, shikimic acid, prussic acid are moored in pears acid, Buddhist nun, and blending ratio is weight ratio of mixture 1: 1: 1: 0.5.
Preferably, described anticorrosion auxiliary material component is for going out pears acid, Buddhist nun moors acetoacetic ester, shikimic acid, the mixture of prussic acid, blending ratio is weight ratio of mixture 1: 1: 1: 0.5, the preparation process of described anticorrosion auxiliary material is: with table balance, take out respectively pears acid, Buddhist nun moors acetoacetic ester, shikimic acid, prussic acid, blending ratio is weight ratio of mixture 1: 1: 1: 0.5, be placed in after the abundant mixing of Erlenmeyer flask, 85% ethanol that adds mixture 30-50 times volume, shake up and staticly settle 12-15 hour, skim liquid level floater, get clear liquid at the middle and upper levels, be anticorrosion auxiliary material, be sealed in black reagent bottle, labelled, be placed in 3-4 ℃ of refrigerator standby.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (9)
1. a substratum that utilizes the oval two eyebrow algaes of canning factory waste water cultivation, it is characterized in that formula is as follows: 90 milliliters of canning factory's waste water, 2 milligrams of water glass, 1 milligram of ferric ammonium citrate, 2 milligrams of ammonium molybdates, 1 milligram of potassiumchromate, 20 milligrams of fish meal, 1 milligram of Xylo-Mucine, 3 milligrams of xanthan gum, 1 milligram of propylene glycol alginate, 5 milligrams of sodium D-isoascorbate, 1.2 milligrams of gluten powders, 0.1 milligram of Tutofusin tris, 1 milligram, urea, 1 milliliter of oxyacetic acid, 1 milliliter of titanium solution, yeast soaks 10 milligrams, powder, 3 milligrams of Sodium desoxycholates, 1.5 milliliters of soil extracts, 1.2 milliliters of ooze leach liquors, 2.5 milliliters of horsehit leach liquors, 1.5 milliliters of chicken manure leach liquors, 1 milligram of L-arginine, 4 milligrams of potassium primary phosphates, 4 milligrams of medical stone powders, 920 plant growth substance 8 international unit, 1 milliliter of vitamin(e) S-3 solution, 1 milliliter of M-F trace element solution, 4 milligrams of Trypsins, 4 milligrams of calcium superphosphate, 6 milligrams of sorbyl alcohols, 3 milligrams, N.F,USP MANNITOL, 2 milligrams of sodium thioglycolates, 1000 milliliters, pure seawater after clorox sterilization.
2. a method of utilizing the oval two eyebrow algaes of canning factory's waste water cultivation, is characterized in that concrete steps are as follows:
Culture vessel is cylindric food grade plastic bucket, 1.5 cubic metres of volumes, high-density polyethylene material is made (market is on sale), colourless or white, there is good light transmission, in order to algae, carry out photosynthesis, bucket is integral type structure, 6 millimeters of wall thickness, there is very large intensity and soundness, one circular lid is arranged at bucket top, on have the silk of revolving, rotatable and seal, 40 centimetres of bucket top cover diameters, lid is stamped 2 holes, gas-filled valve is inserted in a hole, vapor pipe is inserted in another hole, gas-filled valve and vapor pipe are all Glass tubings, 8 millimeters of internal diameters, reactor is washed, after use distilled water rinse 3 times, pack 1000 liters of substratum claimed in claim 1 into, inflation mixes 5 minutes, selection vitality is vigorous, the two eyebrow algae inoculations of free of contamination ellipse, inoculum density 4 * 10
4cell/mL, on lid, insert two clean Glass tubings, one longer, 65 centimetres, 15 centimetres, lid is exposed on top, the logical charge tube in lower end is connected with gas exhausting stone, in order to inflation, (effect of gas exhausting stone is to form very little bubble, avoid the wave of air pocket or rolling to smash frustule), another root is shorter, 30 centimetres, lid is exposed at two ends, in order to exhaust, two same thicknesses of Glass tubing, 8 millimeters of internal diameters, with clean scotch tape, two Glass tubings are fixed, long Glass tubing upper end materials pipe is connected with aerator pump, be filled with mixed gas, mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%, continuous charge, aeration quantity should not be too large, with frustule, can not float, outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 5000-10000Lux, cultivate first 5 days, a simple and easy canopy is built in bucket top, upper placement curtain shuts out the light, make intensity of illumination maintain 1500-2800Lux, the light injury of avoiding sunburst to cause cell, removes curtain on the 6th day, and stronger illumination is conducive to the growth and breeding of cell and synthesizing of high unsaturated fatty acid, be cultured to the 7th day, stop inflation, take away lid, supplement 100 liters of fresh cultures, to make up the moisture evaporating, cover lid, inflation, continues to cultivate, to the 9th day, oval two eyebrow algae density was increased to 250 * 10
4cell/mL, stops cultivating, and from the water tap of bottom, emits algae liquid, after culture vessel is washed, adds disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate.
Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 20～50 parts of pileworts, 10～20 parts of Japanese Honeysuckle Stems, 15～25 parts of eucalyptus leaveses, 5～10 parts of cape jasmines, 5～15 parts, Radix Glycyrrhizae, anticorrosion auxiliary material 1-3 part, disinfection of Chinese drug liquor manufacture method: by fresh pilewort, Japanese Honeysuckle Stem, eucalyptus leaves, Radix Glycyrrhizae, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 80 orders more standby, with table balance, take respectively 120 grams of the pileworts of drying and pulverizing, 60 grams of Japanese Honeysuckle Stems, 60 grams, eucalyptus leaves leaf, 30 grams, Radix Glycyrrhizae, 30 grams of mountain Cape jasmine fruits, 6 grams of anticorrosion auxiliary materials, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 32-36 hour, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.
3. a kind of method of utilizing the oval two eyebrow algaes of canning factory's waste water cultivation according to claim 2, is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
4. a kind of method of utilizing the oval two eyebrow algaes of canning factory's waste water cultivation according to claim 2, is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute.
5. a kind of method of utilizing the oval two eyebrow algaes of canning factory's waste water cultivation according to claim 2, is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute.
6. a kind of method of utilizing the oval two eyebrow algaes of canning factory waste water cultivation according to claim 2, it is characterized in that, to the described reactor of selecting, minute two sterilized phases after washing, first use high pressure nitrogen to spray sterilization, sterilization 2-3 minute, is used high-pressure carbon dioxide to spray sterilization, sterilization 2-3 minute, carries out algae culture again after sterilization.
7. a kind of method of utilizing the oval two eyebrow algaes of canning factory's waste water cultivation according to claim 2, is characterized in that: described anticorrosion auxiliary material is edible salt.
8. a kind of method of utilizing the oval two eyebrow algaes of canning factory waste water cultivation according to claim 2, it is characterized in that: described anticorrosion auxiliary material is the mixture that acetoacetic ester, shikimic acid, prussic acid are moored in pears acid, Buddhist nun, and blending ratio is weight ratio of mixture 1: 1: 1: 0.5.
9. a kind of method of utilizing the oval two eyebrow algaes of canning factory waste water cultivation according to claim 8, it is characterized in that: described anticorrosion auxiliary material component is for going out pears acid, Buddhist nun moors acetoacetic ester, shikimic acid, the mixture of prussic acid, blending ratio is weight ratio of mixture 1: 1: 1: 0.5, the preparation process of described anticorrosion auxiliary material is: with table balance, take out respectively pears acid, Buddhist nun moors acetoacetic ester, shikimic acid, prussic acid, blending ratio is weight ratio of mixture 1: 1: 1: 0.5, be placed in after the abundant mixing of Erlenmeyer flask, 85% ethanol that adds mixture 30-50 times volume, shake up and staticly settle 12-15 hour, skim liquid level floater, get clear liquid at the middle and upper levels, be anticorrosion auxiliary material, be sealed in black reagent bottle, labelled, be placed in 3-4 ℃ of refrigerator standby.
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| CN105441329A (en) * | 2015-12-14 | 2016-03-30 | 临沂大学 | Method for culturing bait-microalgae for young Anthocidaris crassispina by using thermostat water bath cauldron in low-temperature seasons |
| CN105524872A (en) * | 2016-01-29 | 2016-04-27 | 农业部沼气科学研究所 | Application ascorbic acid in improving microalgae biomass |
| CN113998842A (en) * | 2021-11-25 | 2022-02-01 | 石玉龙 | Biological treatment and recovery process for aquaculture wastewater |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104719207A (en) * | 2015-03-24 | 2015-06-24 | 临沂大学 | Method for cultivating branchionus plicatilis through waste plastic buckets |
| CN105441329A (en) * | 2015-12-14 | 2016-03-30 | 临沂大学 | Method for culturing bait-microalgae for young Anthocidaris crassispina by using thermostat water bath cauldron in low-temperature seasons |
| CN105524872A (en) * | 2016-01-29 | 2016-04-27 | 农业部沼气科学研究所 | Application ascorbic acid in improving microalgae biomass |
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| CN113998842A (en) * | 2021-11-25 | 2022-02-01 | 石玉龙 | Biological treatment and recovery process for aquaculture wastewater |
| CN113998842B (en) * | 2021-11-25 | 2023-08-22 | 石玉龙 | Biological treatment and recovery process for cultivation wastewater |
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