CN105176824A - Chlorella culture method - Google Patents
Chlorella culture method Download PDFInfo
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- CN105176824A CN105176824A CN201510076394.3A CN201510076394A CN105176824A CN 105176824 A CN105176824 A CN 105176824A CN 201510076394 A CN201510076394 A CN 201510076394A CN 105176824 A CN105176824 A CN 105176824A
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Abstract
The invention relates to a chlorella culture method. The method utilizes ammonium bicarbonate and calcium superphosphate as the nutrient source, and the ammonium bicarbonate and calcium superphosphate are in a mass ratio of 4.5-5.5:1. The chlorella culture method provided by the invention has the advantages that: (1) chlorella can be directly purified in a natural pond to serve as the algal source without introduction; (2) an ammonium bicarbonate fertilizer and a calcium superphosphate fertilizer can be taken as the culture nutrition source, and nutrient solution preparation is unnecessary; (3) the culture process can realize effective control of miscellaneous algae; and (4) the culture cost is low, and the method can achieve large-scale culture.
Description
Technical field
The present invention relates to field of environment engineering, concrete, relate to a kind of method of cultivating bead.
Background technology
Chlorella (Chlorella) is the general natural disposition unicell green alga of Chlorophyta Chlorella, it is a kind of spherical unicellular fresh water algae, diameter 3 ~ 8 microns, one of life the earliest on the earth, before appearing at more than 20 hundred million years, be a kind of efficient photosynthetic plant, with photoautotrophy growth and breeding, distributed pole is wide.Chlorella is lived in fresh water, and it is by sunlight, water and carbonic acid gas, to divide the vigorous fecundity of 4 cells every 20 hours, ceaselessly solar energy is transformed the frond generating and contain multiple nutritional components, and in propagation, discharges a large amount of oxygen; And its photosynthetic capacity is higher than other plant more than 10 times.
Chlorella is rich in grease, can be used for production biofuel; Also containing some hydrocarbons, gasoline can be processed into after extraction, diesel oil uses.Chlorella is a large amount of nutritive element and heavy metal such as nitrogen, phosphorus absorbed in water in process of growth, can purifying water body.If make it have high fat content and hydrocarbon content with sewage mass propgation chlorella, not only can slow down water quality deterioration, and can be production biomass energy a large amount of high quality raw material are provided.
The production of external chlorella is utilize photoproduction reaction device mostly at present, the research that their research emphasis turns to its deep processing and particular matter to extract by general production technology research, the technology that China Taiwan produces chlorella is also quite ripe, the product of some chlorellas accept by broad masses, although the sixties in 20th century has been carried out research to chlorella on ground in China, but due to scale operation can't be carried out, cost is reduced, so, only rest on the production primary products stage, cause input-output ratio extremely uneven, also constrain the process of it being developed to research simultaneously, in China, the extensive open cultivation model animals amount in ground reaches 1,000 ten thousand/about ml, be subject to dropping into the low restriction of high production, with abroad to cultivate level disparity larger, so the exploration carrying out high density, low cost cultivation is very necessary.
Therefore, be necessary to provide a kind of step that do not need to introduce a fine variety, without the need to configure nutritive medium, utilize nature pond, the large-scale chlorella cultural method of low cost.
Summary of the invention
The object of the invention is to overcome the cost existed in the method for existing cultivation chlorella high, introduce a fine variety difficult defect, provide a kind of operating process easy, without the need to introducing a fine variety, low cost, large-scale chlorella cultural method.
For this reason, the invention provides a kind of method of cultivating chlorella, described method utilizes bicarbonate of ammonia and calcium superphosphate as nutrition source.
The mass ratio of optional described bicarbonate of ammonia and calcium superphosphate is 4.5-5.5:1.
Described in method provided by the present invention, described bicarbonate of ammonia is the ammonium bicarbonate fertilizer of leaded wastewater>=17.1%, water content≤3.5%; Described calcium superphosphate is effective P
2o
5the superphosphate fertilizer of>=12%.Described ammonium bicarbonate fertilizer and superphosphate fertilizer all can be obtained by the mode buying commercially available fertilizer.
Optionally, described method is included in one-level water body carries out initial incubation acquisition initial incubation thing, directly can gather in the crops the chlorella in initial incubation thing, or, described initial incubation thing is seeded in secondary water body and carries out expanding numerous cultivation, wherein, 1000-2000 ten thousand chlorellas are contained in every milliliter of described initial incubation thing.
Described one-level water body and secondary water body all can for be with water fishpond completely by fishes fishing, area is 0.5-5 mu, and the depth of water is less than 1 meter.Wherein, the band water fishpond of described one-level water body to be the light green transparency containing chlorella population be 15-25cm.
Optionally, described initial incubation comprises the following steps: under the temperature of 0-5 DEG C, throwing in nutrition source in one-level water body carrying out chlorella initial incubation to cultivating concentration, cultivate after 2-4 days, throw in chlorinated lime to water body disinfection 20-30 hour.
In method provided by the present invention, before chlorella forms sociales, the temperature keeping water body is needed to be 0-5 DEG C, object is the generation being controlled assorted algae by cold condition, formed after sociales until chlorella, can not requirement be done to the temperature of water body, can state of nature be kept.Wherein, described sociales refer to that in water body, the total amount of chlorella accounts for more than 70% of Measures of Algae in Water Body total amount.
After disinfecting end, again throw in nutrition source to cultivating concentration, continue to cultivate and to add zooplankton and protozoon agent for killing after 2-4 days and fill into nutrition source to cultivating concentration, proceed to cultivate, and every 2 days extra-nutrition sources to cultivating concentration until algal grown obtains initial incubation thing after being 4.5-5.5cm to transparency;
Wherein, described cultivation concentration is the concentration in water body Middle nutrition source (bicarbonate of ammonia and calcium superphosphate mixture) is 7-10mg/L.
Optionally, relative to 1 cubic metre of secondary water body, the addition of described initial incubation thing is 0.1-0.12 cubic meter.
Optionally, the condition of the numerous cultivation of described expansion comprises: inoculate front chlorinated lime and to clean up the pond process to secondary water body, initial incubation thing is added after 5 days, adding nutrition source to concentration is 10-15mg/L, continue to cultivate, every 3 days fill into nutrition source to concentration is 10-15mg/L, uses chlorinated lime and Cypermethrin to kill assorted algae in water and zooplankton, gathers in the crops chlorella when reaching every milliliter of 700-1000 ten thousand to chlorella concentration.
One of the present invention preferred embodiment in, the cycle of described initial incubation is 20-40 days, and the cycle of expanding numerous cultivation is 10-20 days.
Optionally, the addition of chlorinated lime makes the concentration of adding chlorinated lime in chlorinated lime rear stage water body or secondary water body be 7-15mg/L.
Optionally, described initial incubation and the numerous cultivation of expansion are all using dry ice as CO
2source, the addition of dry ice makes the pH value of water body be 6-7.
In method provided by the present invention, the control of assorted algae in initial incubation and the numerous culturing process of expansion is realized mainly through following means:
(1) selecting cold condition (0-5 DEG C) to carry out cultivating this during initial incubation of the present invention is the generation that when make use of low temperature, chlorella growth speed and the less feature of other fresh water algaes control assorted algae.
(2) throw in chlorinated lime at the initial incubation initial stage to carry out disinfection to water body, kill assorted algae (other algae except chlorella), chlorella is trained for a certain area; In described initial incubation with expand in numerous culturing process, when there being assorted algae to occur, concentration is that the chlorinated lime of 7-15mg/L is killed.
(3) easily larger Euglena population is formed when high temperature season in water body, when Euglena occurs, selection has wind weather, carries out local splash and kill (concentration is no more than 20mg/L) at lower air port Euglena oil film forming region unslaked lime and chlorinated lime.
By the method that chlorinated lime adds Cypermethrin (mass ratio 1:1, addition concentration reaches 0.5mg/L), protozoon and zooplankton can be killed before the chlorella that harvesting utilization method provided by the present invention is cultivated.
Utilize method provided by the present invention to carry out chlorella cultivation, have the following advantages: (1) in natural pond directly purification chlorella as algae source, do not need to introduce a fine variety; (2) ammonium bicarbonate fertilizer and superphosphate fertilizer can be used as cultivation nutrition source, do not need to prepare nutritive medium; (3) effectively can control assorted algae in culturing process; (4) toxigenic capacity low, can large scale culturing.
Embodiment
Below will the present invention is described in detail by embodiment.It will be appreciated that providing into being object in order to play explanation of following examples, being not used to limit scope of the present invention.Those skilled in the art, when not deviating from aim of the present invention and spirit, can carry out various amendment and replacement to one's duty invention.
In following examples: transparency utilizes transparent scale-Sa Shi dish to measure.Bicarbonate of ammonia is the bicarbonate of ammonia fertilizer produced purchased from phosphate fertilizer plant of Yonghe County of Hunan Province, and bicarbonate of ammonia is leaded wastewater is >=17.1%, water content is≤3.5%.Calcium superphosphate is the peroxophosphoric acid calcareous fertilisers produced purchased from Anxiang, Hunan Province Jin Mei Taurus Chemical Co., Ltd., effective P
2o
5for>=12%.
Embodiment 1
Pond is selected: pond area about 0.5 mu, dark about 1 meter of pond, and band water fishpond (containing chlorella population, transparency is 15-25cm, and water body is light green), fishes for fish completely, and band water is cleaned up the pond.(method of cleaning up the pond: chlorinated lime full pool spilling head to concentration is 20mg/L, after 3 days, uses after toxicity disappears substantially.)
During temperature 0-3 DEG C, nutrition source is used in one-level water body, in nutrition source, the mass ratio of bicarbonate of ammonia and calcium superphosphate is 5:1), be 7-10mg/L to nutrition source concentration, the concentration adding chlorinated lime in chlorinated lime to water body after 3 days is that 10-15mg/L carries out disinfection, extra-nutrition source to concentration after 24 hours of sterilizing is 7-10mg/L, zooplankton and protozoon is killed with the zooplankton be made up of Avrmectin and Cypermethrin and protozoon agent for killing (weight ratio of Avrmectin and Cypermethrin is 1:1, and in water body, the total concn of the two is 0.5mg/L) after 3 days.Thereafter whole process is 7-10mg/L by 2 days frequency extra-nutrition source to concentration once.Take dry ice as CO
2source is used for pond, and the consumption of dry ice makes water pH value reach 6-7.
Algal grown is inoculated into secondary water body when transparency reaches 5cm to carry out expanding numerous cultivation (containing 1000-2000 ten thousand chlorellas in every milliliter of described initial incubation thing).
The water inlet of secondary water body filters with 300 object bolting silk cloth removes impurity, inoculate front chlorinated lime (concentration of chlorinated lime is that 7-15mg/L carries out disinfection to water body) to clean up the pond process to secondary water body, initial incubation thing is added after 5 days, relative to 1 cubic metre of secondary water body, the addition of described initial incubation thing is 0.12 cubic metre.Adding nutrition source to concentration is 10-15mg/L, continues to cultivate, and every 3 days fill into nutrition source to concentration is 10-15mg/L, uses chlorinated lime and Cypermethrin to kill assorted algae in water and zooplankton, results when chlorella reaches in every milliliter of water body 700-1,000 ten thousand.
Temperature controls: before chlorella forms sociales (in water body, the total amount of chlorella accounts for more than 70% of Measures of Algae in Water Body total amount), and need the temperature keeping water body to be 0-5 DEG C, after chlorella forms sociales, the temperature of water body can not do requirement.
Assorted algae control method: the chlorinated lime when there being assorted algae to occur with 7-15mg/L in culturing process is killed.When high temperature season Euglena occurs, select there is wind weather, splash local in turn at lower air port Euglena oil film forming region unslaked lime and chlorinated lime, consumption is determined, with 20mg/L concentration for reference concentration by Euglena amount.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. cultivate a method for chlorella, it is characterized in that, described method utilizes bicarbonate of ammonia and calcium superphosphate as nutrition source.
2. method according to claim 1, is characterized in that, the mass ratio of described bicarbonate of ammonia and calcium superphosphate is 4.5-5.5:1.
3. method according to claim 1 and 2, it is characterized in that, described method is included in one-level water body carries out initial incubation acquisition initial incubation thing, again described initial incubation thing is seeded in secondary water body and carries out expanding numerous cultivation, wherein, 1000-2000 ten thousand chlorellas are contained in every milliliter of described initial incubation thing.
4. method according to claim 3, it is characterized in that, described initial incubation comprises the following steps: under the temperature of 0-5 DEG C, throwing in nutrition source in one-level water body carrying out chlorella initial incubation to cultivating concentration, cultivate after 2-4 days, throw in chlorinated lime to water body disinfection 20-30 hour, after disinfecting end, again throw in nutrition source to cultivating concentration, continue to cultivate and to add zooplankton and protozoon agent for killing after 2-4 days and fill into nutrition source to cultivating concentration, proceed to cultivate, and every 2 days extra-nutrition sources to cultivating concentration until algal grown obtains initial incubation thing after being 4.5-5.5cm to transparency,
Wherein, described cultivation concentration is the concentration in water body Middle nutrition source is 7-10mg/L.
5. the method according to claim 1,2 or 4, is characterized in that, described bicarbonate of ammonia is the ammonium bicarbonate fertilizer of leaded wastewater>=17.1%, water content≤3.5%; Described calcium superphosphate is effective P
2o
5the superphosphate fertilizer of>=12%.
6. method according to claim 5, is characterized in that, relative to 1 cubic metre of secondary water body, the addition of described initial incubation thing is 0.1-0.12 cubic meter.
7. the method according to claim 4 or 6, is characterized in that, the condition of the numerous cultivation of described expansion comprises:
Inoculate front chlorinated lime to clean up the pond process to secondary water body, initial incubation thing is added after 5 days, adding nutrition source to concentration is 10-15mg/L, continue to cultivate, every 3 days fill into nutrition source to concentration is 10-15mg/L, use chlorinated lime and Cypermethrin to kill assorted algae in water and zooplankton, when reaching every milliliter of 700-1000 ten thousand to chlorella concentration, gather in the crops chlorella.
8. method according to claim 7, is characterized in that, using dry ice as CO
2source, the addition of dry ice makes the pH value of water body be 6-7.
9. method according to claim 8, is characterized in that, the concentration of adding chlorinated lime in chlorinated lime rear stage water body or secondary water body is 7-15mg/L.
10. method according to claim 8 or claim 9, it is characterized in that, the process of described initial incubation and the numerous cultivation of expansion also comprises the control of Euglena, the method that described Euglena controls comprises: when Euglena occurs, selection has wind weather, carries out local splash at lower air port Euglena oil film forming region unslaked lime and chlorinated lime.
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| CN201510076394.3A CN105176824A (en) | 2015-02-12 | 2015-02-12 | Chlorella culture method |
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| CN201510076394.3A CN105176824A (en) | 2015-02-12 | 2015-02-12 | Chlorella culture method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753623A (en) * | 2018-06-12 | 2018-11-06 | 兰溪市沉默生物科技有限公司 | A kind of chlorella store method |
| CN113229134A (en) * | 2021-05-28 | 2021-08-10 | 肥东县水产养殖技术服务中心 | Method for fertilizing water culture algae in fishpond |
| CN113881569A (en) * | 2021-09-15 | 2022-01-04 | 广东海元农业科技有限公司 | Method for cultivating seawater chlorella in cement pond and method for cultivating grouper fries |
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Patent Citations (4)
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| CN101363004A (en) * | 2008-09-04 | 2009-02-11 | 中国水产科学研究院南海水产研究所 | Method for constructing algal phase in prawn culturing pool |
| CN101731158A (en) * | 2008-11-24 | 2010-06-16 | 张明钰 | Shallow pond culture technique for sinonovacula constricta lamarck |
| CN102487861A (en) * | 2011-11-25 | 2012-06-13 | 王兴春 | High-yield high-efficiency technology for cultivating sinonovacula constricta in pond |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108753623A (en) * | 2018-06-12 | 2018-11-06 | 兰溪市沉默生物科技有限公司 | A kind of chlorella store method |
| CN113229134A (en) * | 2021-05-28 | 2021-08-10 | 肥东县水产养殖技术服务中心 | Method for fertilizing water culture algae in fishpond |
| CN113881569A (en) * | 2021-09-15 | 2022-01-04 | 广东海元农业科技有限公司 | Method for cultivating seawater chlorella in cement pond and method for cultivating grouper fries |
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Application publication date: 20151223 |