CN103805204B - A kind of agricultural land soil microorganism water conservation goods and preparation method thereof - Google Patents
A kind of agricultural land soil microorganism water conservation goods and preparation method thereof Download PDFInfo
- Publication number
- CN103805204B CN103805204B CN201310307877.0A CN201310307877A CN103805204B CN 103805204 B CN103805204 B CN 103805204B CN 201310307877 A CN201310307877 A CN 201310307877A CN 103805204 B CN103805204 B CN 103805204B
- Authority
- CN
- China
- Prior art keywords
- goods
- soil
- water conservation
- culture
- cctccno
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to a kind of agricultural land soil microorganism water conservation goods and the method preparing these goods by the functional microorganism bacterial strain of a strain synthesis glutamic acid and the functional microorganism bacterial strain of strain synthesis -polyglutamic acid, belong to microbial technology field. Does is the microbial strains of the present invention brevibacterium flavum? IAE(<i>brevibacterium</i><i>flavum? IAE</i>) and bacillus amyloliquefaciens IAE(<i>bacillus? amyloliquefaciens? IAE</i>), it is isolatable from tidal flat of Jiangsu Province salt affected soil. Two bacterial strains are with the pig manure become thoroughly decomposed, rice straw, (NH4)2SO4Microorganism water conservation goods are produced for solid fermentation substrate, does is the effective water conservation material of these goods higher than 19.6g? kg-1Dry matter weight. Field soil water retention laboratory is shown by these goods, and this microbial organic fertilizer can reduce the soil weight, increase soil hydraulic conductivity and saturated soil water content after being manured into soil, by increasing capacitance it is possible to increase plant plant height, fresh weight, dry weight and yield.
Description
One, technical field
The present invention relates to the microbial strains preparing agricultural soil water-keeping material and by the microorganism water conservation goods that solid fermentation produces, belong to microbial technology field.
Two, background technology
Agricultural is that China uses water rich and influential family, its water consumption accounts for about the 70% of whole nation water total amount, agricultural year hydropenia about 30,000,000,000 cubes, develop technical development of efficiently retaining and conserveing soil moisture and account for about 56% dry farming of territory land surface, make full use of precipitation and subsoil water (including the soil water) Developing Water-saving Agriculture, being the fundamental way of agricultural modernization development, application water-keeping material is retained and conserved soil moisture and made full use of water resource is realize one of water-saving agriculture important channel.
The microbial organic fertilizer of the water suction water storage high molecular polymer of microbial solid fermentation synthesis, there is degradable, environmental friendliness and high added valueization can process solid organic castoff and substantially reduce the advantage of widespread pollution from the overuse of fertilizers and pesticides in rural area, being a study hotspot in water-saving agriculture field. Fermentable synthetic product ��-polyglutamic acid (poly-��-glutamicacid) is a kind of macromole long-chain polypeptide, molecular weight is at 30000D ~ 50000D, water absorbent rate is up to 3500 times, and repetition good water absorption, it is widely used in the fields such as food, cosmetics, life absorbent material.
Major part ��-PGA high yield microorganism is glutamic acid (Glu) dependent form bacterial strain, and solid fermentation substrate needs to add rich in glutamic acid-type material, and such as Semen Glycines powder, casein, glutamic acid or residue of monosodium glutamate etc., production cost is higher. ��-polyglutamic acid is a kind of natural macromolecular substances, easily degrades in soil, causes that Water-saving effect reduces.
Microorganism can utilize inorganic nitrogen-sourced (carbamide, ammonium sulfate etc.) synthesis glutamic acid-type material, biological water-keeping goods are made by synthesizing combined ferment (co-fermentation) solid organic castoff of glutamic acid microorganism and synthesis ��-polyglutamic acid microorganism, cost of material is low, and continues to synthesize glutamic acid in soil by the glutamic acid synthesized micro-organism being carried along into soil and can effectively maintain the stability of soil ��-polyglutamic acid;Adopt the ��-PGA biological organic fertilizer that microbial solid fermentation agricultural organic waste and inorganic nitrogenous source produce, it is possible to continue to supply microorganism energy by organic matter and reduce functional microorganism extinction in soil, extend the Water-saving effect of goods simultaneously. By combined ferment explained hereafter microbe soil water conservation goods, high added value can utilize agricultural wastes, can effectively increasing soil moisture to keep, be conducive to improving the utilization ratio of agricultural water resources, made microorganism water conservation goods will have good application prospect.
Three, summary of the invention
Goal of the invention
It is an object of the invention to provide the microbial strains of combined ferment agricultural wastes and inorganic nitrogen-sourced production soil water-retaining goods and soil water-retaining goods that fermentation is made, utilize for agricultural production, develop high-efficiency water-saving modern agriculture.
Technical scheme
One agricultural land soil microorganism water conservation goods of the present invention and preparation method thereof are realized by below scheme:
A kind of agricultural land soil microorganism water conservation goods, it is characterised in that described a kind of agricultural land soil microorganism water conservation goods are a strain synthesis corynebacterium glutamicum strain and the strain synthesis pig manure of the strain-combined fermentation maturity of ��-polyglutamic acid, rice straw powder and (NH4)2SO4The water conservation goods obtained;
Wherein said corynebacterium glutamicum strain is brevibacterium flavum IAE(BrevibacteriumflavumIAE), on March 15th, 2013 is preserved in China typical culture collection center, and preservation address is Luo Jia Shan, wuchang, wuhan, and culture presevation number is CCTCCNO:M2013087; Referred to as brevibacterium flavum CCTCCNO:M2013087;
Described ��-many corynebacterium glutamicum strains are bacillus amyloliquefaciens IAE(BacillusamyloliquefaciensIAE), on March 15th, 2013 is preserved in China typical culture collection center, and preservation address is Luo Jia Shan, wuchang, wuhan, and culture presevation number is CCTCCNO:M2013086, referred to as bacillus amyloliquefaciens CCTCCNO:M2013086.
In these goods, ��-polyglutamic acid content is higher than 19.6gkg-1Dry products, containing 0.5 �� 108cfug-1Above brevibacterium flavum CCTCCNO:M2013087 colony-forming units and 0.5 �� 108cfug-1Above bacillus amyloliquefaciens CCTCCNO:M2013086 colony-forming units, quality of organic matter is 30��35% than content, and moisture content is lower than 30%.
The condition of culture of described brevibacterium flavum CCTCCNO:M2013087: the bacterium growing 24 hours with inoculating loop picking from culture dish is inoculated in equipped with in the 250ml triangular flask of 100ml culture fluid, 37 DEG C, 170rmp cultivate 48 hours, bacterium solution supernatant 10000rpm is centrifuged 20min, and measuring supernatant content of glutamic acid is 26.6gL-1��
Used by brevibacterium flavum CCTCCNO:M2013087, culture fluid compound method is, to prepare 1L culture medium: glucose 50g, and Semen Maydis pulp 10ml, carbamide 5g, KH2PO43g, MgSO40.8g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min.
The condition of culture of described bacillus amyloliquefaciens CCTCCNO:M2013086: the bacterium growing 24 hours with inoculating loop picking from culture dish is inoculated in the 250ml triangular flask synthesizing glutamic acid culture fluid equipped with 100ml, 37 DEG C, 170rmp cultivate 48 hours, bacterium solution supernatant 10000rpm is centrifuged 20min, and measuring supernatant ��-polyglutamic acid content is 21.8gL-1��
Fluid medium compound method used by described bacillus amyloliquefaciens CCTCCNO:M2013086 is, to prepare 1L culture medium: glucose 80g, and sodium glutamate 50g, (NH4)2SO48g, NaCl5g, K2HPO4��3H2O2g, MnSO4��7H2O0.25g, MnSO4H2O0.03g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min.
The preparation method of described soil microorganism water conservation goods, it is characterised in that realize as follows:
1) brevibacterium flavum CCTCCNO:M2013087 bacterium being inoculated into liquid seed culture medium cultivate, condition of culture is: cultivation temperature 37 DEG C, shaking speed 150rpm, and incubation time 24 hours, seed bacteria containing amount is more than 1 �� 109cfuml-1, seed is inoculated into fermentation tank according to 5% ratio and carries out liquid fermentation production, and the condition of its fermenting and producing is: cultivation temperature 28 ~ 33 DEG C, and dissolved oxygen ventilation ranges for 30��100%, 150 ~ 220rpm, colony-forming units >=1 �� 10 of fermentation later stage fermentation this bacterial strain of liquid9cfuml-1. The formula of the seed culture medium of liquid used is, prepares according to 1L culture medium: Carnis Bovis seu Bubali cream 3g, peptone 10g, NaCl10g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min; Liquid fermentation medium formula used is, prepares according to 1L culture medium: glucose 50g, Semen Maydis pulp 10ml, carbamide 5g, KH2PO43g, MgSO40.8g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min;
2) bacillus amyloliquefaciens CCTCCNO:M2013086 being inoculated into liquid seed culture medium cultivate, condition of culture is: cultivation temperature 37 DEG C, shaking speed 130 ~ 160rpm, and incubation time 24 hours, seed bacteria containing amount is more than 1 �� 109cfuml-1, seed is inoculated into fermentation tank according to 8% ratio and carries out liquid fermentation production, and the condition of its fermenting and producing is: cultivation temperature 28 ~ 35 DEG C, dissolved oxygen ventilation ranges for 30��100%, 120 ~ 160rpm, fermentation latter portions bacterial strain forms spore, colony-forming units >=1 �� 10 of this bacterial strain of fermentation liquid9cfuml-1, fermentation liquid concentration retrogradation; The formula of the seed culture medium of liquid used is, prepares according to 1L culture medium: Carnis Bovis seu Bubali cream 3g, peptone 10g, NaCl10g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min. Liquid fermentation medium formula used is, prepares according to 1L culture medium: glucose 80g, sodium glutamate 50g, (NH4)2SO48g, NaCl5g, K2HPO4��3H2O2g, MnSO4��7H2O0.25g, MnSO4H2O0.03g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min;
3) by brevibacterium flavum CCTCCNO:M2013087 fermentation liquid, bacillus amyloliquefaciens CCTCCNO:M2013086 fermentation liquid, (NH4)2SO4, rice straw powder and the pig manure that becomes thoroughly decomposed be according to 5 ~ 8:6 ~ 10:7 ~ 13:25 ~ 35:40 ~ 55(w:w:w:w:w:w) stirring and evenly mixing thoroughly, bar buttress formula is banked up fermentation, fermentation temperature is 30 ~ 50 DEG C, turning in every 3 days 1 time in sweat, terminate after fermenting 15 ~ 20 days, make brevibacterium flavum content reach 0.5 �� 108cfug-1Above, bacillus amyloliquefaciens content reaches 0.5 �� 108cfug-1Above, bacterial strain stable content, ��-polyglutamic acid content is higher than 19.6gkg-1, it is thus achieved that goods be agricultural soil microorganism water conservation goods;
Described microbe soil water conservation goods can be used for agricultural crops planting soil, increases water retention in soil, improves water resource utilization efficiency, increases plant products.
Beneficial effect
The present invention provide a kind of can adapt to arid lean soil environment, glutamic acid can be synthesized and the microbial strains of ��-polyglutamic acid and the production method of agricultural soil water conservation goods thereof can be synthesized. Efficiently synthesize corynebacterium glutamicum strain by the separation of synthesis glutamic acid and ��-polyglutamic acid microorganism in long-term hydropenia arid and lean soil environment is obtained a strain and efficiently synthesize ��-polyglutamic acid bacterial strain, utilize the fermentation in agricultural solid residue of this two strains bacterial strain and stability program, making the microbial product with water retention, its goods compare with market goods and have the advantage that
1. this bacterial strain has the ability adapting to arid and lean soil environment, result of the test shows, use the beach saline-alkali soil environment of these goods, after arid winter and 6 months spring, the brevibacterium flavum bacterial strain and the synthesis ��-polyglutamic acid Bacillus amyloliquefaciens strain survival volume that synthesize glutamic acid in soil are respectively greater than 60% and 80%, can continue to synthesize water conservation material, ��-polyglutamic acid compound single compared to soil application or single bacillus amyloliquefaciens water conservation goods, the Water-saving effect persistent period is more of a specified duration, water holding capacity is higher.
2. these goods contain abundant water conservation material, by increasing capacitance it is possible to increase soil macro aggregate quantity, the minimizing soil weight, minimizing top layer skinning, increase soil saturation/unsaturated hydraulic conductivity, the effectiveness that moisturizes, increase specific water capacity.
3. owing to being biological product, entirely without the series of problems brought because of the use of chemicals, be conducive to improving the sustainable development of agricultural water use efficiency.
Four, accompanying drawing explanation
Fig. 1 is brevibacterium flavum bacterial strain electromicroscopic photograph.
Fig. 2 is Bacillus amyloliquefaciens strain electromicroscopic photograph.
Fig. 3 is �� after purification-polyglutamic acid soaking effect figure.
Fig. 4 is the moisture-preservation growth-promoting design sketch of water conservation goods.
Five, detailed description of the invention
Embodiment 1
The separation of bacterial strain and qualification
Gather the plant rhizosphere surrounding soil cryopreservation in the exposed beach of tidal flat of Jiangsu Province, sporadicly grown, Soil Microorganism is adopted basis beef-protein medium separation glutamic acid synthesized micro-organism bacterial strain, the preparation of its culture medium measures compound method according to 1L: Carnis Bovis seu Bubali cream 3g, peptone 10g, NaCl10g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min.
Measure, by liquid culture, the concentration screening synthesizing glutamic acid further and efficiently synthesize bacterial strain. Condition of culture: inoculated and cultured ware cultivates the bacterial strain of 24h in equipped with in the 250ml triangular flask of 100ml fluid medium, inoculum concentration 104cfuml-1, 37 DEG C, 150rmp cultivate 7 days, bacterium solution supernatant 10000rpm be centrifuged 20min, the aminoglutaric acid concentration in mensuration culture fluid, it is thus achieved that the maximum bacterial strain of glutamic acid synthetic quantity, fermentation liquid aminoglutaric acid concentration 26.6gL-1. This bacterial strain belongs to brevibacterium flavum IAE(BrevibacteriumflavumIAE), on March 15th, 2013 is preserved in China typical culture collection center, preservation address is Luo Jia Shan, wuchang, wuhan, and culture presevation number is CCTCCNO:M2013087, and Main Biological is: bacterium colony is faint yellow, rod-short, Gram-positive, amphimicrobian does not move, atrichia, without spore, the electromicroscopic photograph of bacterial strain is as shown in Figure 1; Through the evolutionary analysis of 16srDNA sequence, result be shown as brevibacterium flavum (B.flavum).
Above-mentioned synthesis glutamic acid liquid culture based formulas is: glucose 50g, Semen Maydis pulp 10ml, carbamide 5g, KH2PO43g, MgSO40.8g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min.
Gather the plant rhizosphere surrounding soil cryopreservation in the exposed beach of tidal flat of Jiangsu Province, sporadicly grown, adopt glutamic acid culture medium separating gamma-polyglutamic acid synthesized micro-organism bacterial strain. The standard of screening is that bacterium colony forms high protuberance in culture medium, and bulge has very strong viscosity and absorptive bacterial strain. The strongest bacterial strain of ability of screening liquid fermentation synthesis ��-polyglutamic acid, this bacterial strain synthesis ��-polyglutamic acid amount is 21.8gL-1; This bacterial strain is bacillus amyloliquefaciens IAE(BacillusamyloliquefaciensIAE), on March 15th, 2013 is preserved in China typical culture collection center, and preservation address is Luo Jia Shan, wuchang, wuhan, culture presevation number is CCTCCNO:M2013086, and Main Biological is: thalline is shaft-like, even dyeing, tool mobility, amphimicrobian, spore ovalize, Gram-positive, the white opaque colony of bacterium colony in culture medium, edge is irregular, has protuberance, surface folding, bacterial strain electromicroscopic photograph is as shown in Figure 2; Through the evolutionary analysis of 16srDNA sequence, result be shown as bacillus amyloliquefaciens (B.amyloliquefaciens).
The formula of above-mentioned culture medium is, according to preparation 1L amount preparation: glucose 80g, sodium glutamate 50g, (NH4)2SO48g, NaCl5g, K2HPO4��3H2O2g, MnSO4��7H2O0.25g, MnSO4H2O0.03g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min.
Microbial inoculum produces
Brevibacterium flavum CCTCCNO:M2013087 being inoculated into liquid seed culture medium cultivate, condition of culture is: cultivation temperature 37 DEG C, shaking speed 150rpm, and incubation time 24 hours, seed bacteria containing amount is more than 1 �� 109cfuml-1, seed is inoculated into fermentation tank according to 5% ratio and carries out liquid fermentation production, and the condition of its fermenting and producing is: cultivation temperature 28 ~ 33 DEG C, and dissolved oxygen ventilation ranges for 30��100%, 150 ~ 220rpm, colony-forming units >=1 �� 10 of fermentation later stage fermentation this bacterial strain of liquid9cfuml-1; The seed culture medium of liquid used and the formula of fermentation medium are prepare according to 1L culture medium: Carnis Bovis seu Bubali cream 3g, peptone 10g, NaCl10g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min.
Bacillus amyloliquefaciens CCTCCNO:M2013086 being inoculated into liquid seed culture medium cultivate, condition of culture is: cultivation temperature 37 DEG C, shaking speed 130 ~ 160rpm, and incubation time 24 hours, seed bacteria containing amount is more than 1 �� 109cfuml-1, seed is inoculated into fermentation tank according to 8% ratio and carries out liquid fermentation production, and the condition of its fermenting and producing is: cultivation temperature 28 ~ 35 DEG C, dissolved oxygen ventilation ranges for 30��100%, 120 ~ 160rpm, fermentation latter portions bacterial strain forms spore, colony-forming units >=1 �� 10 of this bacterial strain of fermentation liquid9cfuml-1, fermentation liquid concentration retrogradation. The formula of liquid seed culture medium used is, prepares according to 1L culture medium: Carnis Bovis seu Bubali cream 3g, peptone 10g, NaCl10g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min. Liquid fermentation medium formula used is, prepares according to 1L culture medium: glucose 80g, sodium glutamate 50g, (NH4)2SO48g, NaCl5g, K2HPO4��3H2O2g, MnSO4��7H2O0.25g, MnSO4H2O0.03g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min.
Agricultural soil microorganism water conservation production of articles
By brevibacterium flavum CCTCCNO:M2013087 fermentation liquid, bacillus amyloliquefaciens CCTCCNO:M2013086 fermentation liquid, (NH4)2SO4, rice straw powder and the pig manure that becomes thoroughly decomposed be according to 5 ~ 8:6 ~ 10:7 ~ 13:25 ~ 35:40 ~ 55(w:w:w:w:w:w) stirring and evenly mixing thoroughly, bar buttress formula is banked up fermentation, fermentation temperature is 30 ~ 50 DEG C, turning in every 3 days 1 time in sweat, terminate after fermenting 15 ~ 20 days, make brevibacterium flavum CCTCCNO:M2013087 content reach 0.5 �� 108cfug-1Above, bacillus amyloliquefaciens CCTCCNO:M2013086 content reaches 0.5 �� 108cfug-1Above, bacterial strain stable content, ��-polyglutamic acid content is higher than 19.6gkg-1, it is thus achieved that goods be agricultural soil microorganism water conservation goods.
Agricultural soil water conservation goods Water-saving effect is verified
It is aleuritic texture soil for examination soil, is Brassica campestris L for studying thing.
It is as follows that test arranges process: processes 1. comparisons; Process 2. application of organic fertilizers; Process 3. and use microorganism water conservation goods. Processing 1 soil for comparison, processing 2 soil according to mass ratio 10% adds fertilizer, processes 3 soil according to mass ratio 10% and adds microorganism water conservation goods, and fertilizer, water conservation goods all stir with soil. Stop time the amount that three process are watered occurs water saturated with reference to control treatment soil watering, it has been found that three process plant and all show time hydropenia is wilted and start to water. Measure the plant height of plant, fresh weight and dry weight in Fructus Capsici period of duration, the soil weight, hydraulic conductivity, saturated aqueous rate after Fructus Capsici results.
Test result indicate that, rapeseed cultivation is after 60 days, and the average plant height of control treatment plant, fresh weight and dry weight are 17.6cm, 98.9g, 6.7g;It is 18.2cm, 100.5g, 7.2g that application of organic fertilizers processes the average plant height of plant, fresh weight and dry weight; It is 22.8cm, 125.7g, 9.8g that microorganism water conservation goods process the average plant height of plant, fresh weight and dry weight; Compared to control treatment 1, using the microorganism water conservation goods process average plant height of plant, fresh weight and dry weight increases by 27.8%, 27.1% and 36.1% respectively; Compared to processing 2, using the microorganism water conservation goods process average plant height of plant, fresh weight and dry weight increases by 25.3%, 25.1% and 46.2% respectively. Comparison, fertilizer and microorganism water conservation goods process the soil weight respectively 1.32g/cm3��1.27g/cm3��1.22g/cm3, soil hydraulic conductivity is 5.2mm/h, 5.8mm/h, 6.7mm/h respectively, saturation moisture content respectively 36%, 39%, 46%, compared to control treatment, microorganism water conservation goods process the soil weight and have dropped 7.5%, and soil hydraulic conductivity adds 28.8%, and saturation moisture content increases by 27.8%.
Claims (5)
1. agricultural land soil microorganism water conservation goods, it is characterised in that described a kind of agricultural land soil microorganism water conservation goods are a strain synthesis corynebacterium glutamicum strain and the strain synthesis pig manure of the strain-combined fermentation maturity of ��-polyglutamic acid, rice straw powder and (NH4)2SO4The water conservation goods obtained;
Wherein said synthesis corynebacterium glutamicum strain is brevibacterium flavum Brevibacteriumflavum, and on March 15th, 2013 is preserved in China typical culture collection center, and preservation address is Luo Jia Shan, wuchang, wuhan, and culture presevation number is CCTCCNO:M2013087;
Described synthesis ��-polyglutamic acid bacterial strain is bacillus amyloliquefaciens Bacillusamyloliquefaciens, on March 15th, 2013 is preserved in China typical culture collection center, preservation address is Luo Jia Shan, wuchang, wuhan, and culture presevation number is CCTCCNO:M2013086.
2. a kind of agricultural land soil microorganism water conservation goods according to claim 1, it is characterised in that in these goods, ��-polyglutamic acid content is higher than 19.6g kg-1Dry products, containing 0.5 �� 108cfu��g-1Above brevibacterium flavum CCTCCNO:M2013087 colony-forming units and 0.5 �� 108cfu��g-1Above bacillus amyloliquefaciens CCTCCNO:M2013086 colony-forming units, quality of organic matter is 30��35% than content, and moisture content is lower than 30%.
3. a kind of agricultural land soil microorganism water conservation goods according to claims 1 or 2, it is characterized in that the condition of culture of brevibacterium flavum CCTCCNO:M2013087: the bacterium growing 24 hours with inoculating loop picking from culture dish is inoculated in equipped with in the 250ml triangular flask of 100ml culture fluid, 37 DEG C, 170rmp cultivate 48 hours, bacterium solution supernatant 10000rpm is centrifuged 20min, and measuring supernatant content of glutamic acid is 26.6g L-1��
4. a kind of agricultural land soil microorganism water conservation goods described according to claim 3, it is characterized in that used by brevibacterium flavum CCTCCNO:M2013087, culture fluid compound method is, to prepare 1L culture medium: glucose 50g, Semen Maydis pulp 10ml, carbamide 5g, KH2PO43g, MgSO40.8g, distilled water 1000ml, pH value is natural, 115 DEG C of sterilizing 30min.
5. a kind of agricultural land soil microorganism water conservation goods according to claim 1 or claim 2, it is characterized in that the condition of culture of bacillus amyloliquefaciens CCTCCNO:M2013086: the bacterium growing 24 hours with inoculating loop picking from culture dish is inoculated in the 250ml triangular flask synthesizing ��-polyglutamic acid culture fluid equipped with 100ml, 37 DEG C, 170rmp cultivate 48 hours, bacterium solution supernatant 10000rpm is centrifuged 20min, and measuring supernatant ��-polyglutamic acid content is 21.8g L-1��
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310307877.0A CN103805204B (en) | 2013-07-22 | 2013-07-22 | A kind of agricultural land soil microorganism water conservation goods and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310307877.0A CN103805204B (en) | 2013-07-22 | 2013-07-22 | A kind of agricultural land soil microorganism water conservation goods and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103805204A CN103805204A (en) | 2014-05-21 |
| CN103805204B true CN103805204B (en) | 2016-06-08 |
Family
ID=50702603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310307877.0A Active CN103805204B (en) | 2013-07-22 | 2013-07-22 | A kind of agricultural land soil microorganism water conservation goods and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103805204B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762087B (en) * | 2015-01-21 | 2018-03-13 | 河海大学 | It is a kind of to be used to improve microorganism additive of beach saline-alkali soil texture and its preparation method and application |
| CN104744182B (en) * | 2015-01-21 | 2017-07-18 | 河海大学 | One grows tobacco water conservation biological organic fertilizer and preparation method thereof |
| CN104629768B (en) * | 2015-01-22 | 2017-12-15 | 河海大学 | A kind of microbial product of with reducing beach saline-alkali surface soil alkalinity |
| CN104671432B (en) * | 2015-01-27 | 2016-08-31 | 河海大学 | A kind of water body purification of aquaculture agent and preparation method thereof |
| CN107058151B (en) * | 2016-11-29 | 2021-08-20 | 广东森度生态农业科技有限公司 | Microbial bacterium with fermentation water retention function and application thereof |
| CN107236685B (en) * | 2017-05-22 | 2020-06-02 | 中国科学院成都生物研究所 | Method for producing gamma-polyglutamic acid organic fertilizer by using sludge |
| CN107318431A (en) * | 2017-07-26 | 2017-11-07 | 安徽咱家田生态农业有限公司 | A kind of method that utilization root division method cultivates Snakegourd Fruit seedling |
| CN110606782A (en) * | 2019-09-30 | 2019-12-24 | 广东森度生态农业科技有限公司 | Farmland soil water-retaining organic fertilizer and preparation method thereof |
| CN111296230B (en) * | 2020-03-13 | 2021-03-19 | 河海大学 | Ecological base material for mangrove planting engineering and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389543A (en) * | 2001-06-05 | 2003-01-08 | 陈健行 | Activating compost as soil improver |
| CN101948769A (en) * | 2010-08-20 | 2011-01-19 | 南京农业大学 | Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof |
| CN102002234A (en) * | 2010-10-22 | 2011-04-06 | 秦皇岛领先科技发展有限公司 | Method for preparing water-retaining agent |
| CN102383415A (en) * | 2011-07-23 | 2012-03-21 | 天津北洋百川生物技术有限公司 | Method for controlling desertification by adopting novel biological material gamma-polyglutamic acid (PGA) |
| CN103172437A (en) * | 2012-12-31 | 2013-06-26 | 天津北洋百川生物技术有限公司 | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003023850A (en) * | 2001-07-13 | 2003-01-28 | Nisshoku Corp | Growth accelerator and growth acceleration method each for greening plant on strongly acidic soil slope or strongly alkaline soil slope |
-
2013
- 2013-07-22 CN CN201310307877.0A patent/CN103805204B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389543A (en) * | 2001-06-05 | 2003-01-08 | 陈健行 | Activating compost as soil improver |
| CN101948769A (en) * | 2010-08-20 | 2011-01-19 | 南京农业大学 | Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof |
| CN102002234A (en) * | 2010-10-22 | 2011-04-06 | 秦皇岛领先科技发展有限公司 | Method for preparing water-retaining agent |
| CN102383415A (en) * | 2011-07-23 | 2012-03-21 | 天津北洋百川生物技术有限公司 | Method for controlling desertification by adopting novel biological material gamma-polyglutamic acid (PGA) |
| CN103172437A (en) * | 2012-12-31 | 2013-06-26 | 天津北洋百川生物技术有限公司 | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 产聚γ-谷氨酸菌株的筛选;邵丽;《山东轻工业学院硕士学位论文》;20081231;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103805204A (en) | 2014-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103805204B (en) | A kind of agricultural land soil microorganism water conservation goods and preparation method thereof | |
| CN101941851B (en) | Technology and process for preparing biochemical humic acid by using kitchen waste | |
| CN104609995B (en) | Plant growth promoting bio-organic fertilizer for saline-alkali land | |
| CN101987799B (en) | Artificial turf as well as manufacturing method and use method thereof | |
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN102992827B (en) | Active organic bacterial fertilizer and preparation method thereof | |
| CN105176881B (en) | A method of producing the engineering microbial inoculum and its production active bio-organic fertilizer of active bio-organic fertilizer | |
| CN104261983B (en) | A kind of preparation method of cultivation matrix | |
| CN103937695B (en) | A kind of composite bacteria agent and manufacture method thereof of processing livestock and poultry cultivation sewage | |
| CN103011916B (en) | Method for preparing organic bacteria liquid, organic bacteria liquid prepared by method and application of organic bacteria liquid | |
| CN103553828A (en) | Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof | |
| CN104788143B (en) | A kind of utilization acid hydrolysis solution produces the method and product of biological organic fertilizer with decomposed manure | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN103011961A (en) | Active organic bacterial manure and preparation method thereof | |
| CN103396182B (en) | Method for producing solanaceous vegetable seedling growing substrate by utilizing salvaged material of cassava processing | |
| CN108911892A (en) | A kind of sheep dung organic fertilizer and preparation method thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN107129327A (en) | Microbial manure and preparation method thereof | |
| CN1924004A (en) | Organism preparation method of agricultural amino acid and fertilizer product | |
| CN104447054A (en) | Special biological organic fertilizer for tea | |
| CN102942420B (en) | Special microbial agent and bio-organic fertilizer for sweet cherry and preparation methods of special microbial agent | |
| CN109320355A (en) | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application | |
| CN104232532A (en) | Endophytic bacillus pumilus, and microbial agent, preparation method and application of endophytic bacillus pumilus | |
| CN105420169A (en) | Gamma-polyglutamic acid producing strain and method for producing gamma-polyglutamic acid organic fertilizer | |
| CN105948842B (en) | Livestock and poultry dung recovery produces lichens micro-organisms bacillus fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |