CN101948769A - Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof - Google Patents
Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof Download PDFInfo
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Abstract
The invention discloses bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof, and belongs to the technical field of fermentation of microorganisms. In the invention, a bacterial strain YXY-C1 is used for preparing the gamma-polyglutamic acid efficiently by separation; and by using cow dung and monosodium glutamate meal as main raw materials and bran, corn meal, bean meal, citric acid, MgSO4.7H2O and MnSO4.H2O as auxiliary materials, the bacterial strain YXY-C1 can prepare the gamma-polyglutamic acid by solid fermentation, the yield is 39 grams per kilogram of culture medium, and the molecular weight is 300kD. The bacterial strain YXY-C1 can prepare the gamma-polyglutamic acid by fermenting solid wastes and low-price agricultural and sideline products, and has the advantages of low raw material cost, simple method, high yield, convenient product separation and purification and potential application prospect.
Description
One, technical field
The invention belongs to the microbial fermentation technology field, be specifically related to a kind of solid fermentation and produce bacterium YXY-C1 of γ-polyglutamic acid and products thereof.
Two, background technology
γ-polyglutamic acid be by microorganism utilize D-and/or L-type L-glutamic acid monomer by be polymerized a kind of natural electronegative of the amido linkage between alpha-amino group and the γ-carboxyl and high molecular polymer, find in the pod membrane of Bacillus anthracis by Ivanovics and co-worker thereof at first, receive increasing concern in recent decades.γ-polyglutamic acid has high water soluble and suction moisture retention, and biodegradable, edible, nontoxic to human and environment, so γ-polyglutamic acid and derivative thereof have great exploitation in food, chemical industry, material, medicine and other fields and are worth and wide application prospect.
The typical strain that is used at present studying γ-polyglutamic acid production both at home and abroad mainly contains Bacillus licheniformisATCC9945a (separate obtain from soil) and Bacillus subtilis IFO3335 (separate and obtain) etc. from bean product.And the method for producing γ-polyglutamic acid has two kinds of liquid fermenting and solid fermentations usually.Be compared to liquid fermenting, solid fermentation have low, the device simple of power consumption, cost low, produce few, the advantages such as output is high, organic efficiency height of waste, so be usually used in the middle of the microbial fermentation industrial production such as feed, food, the energy.
On the other hand, the feces of livestock and poultry behind the large-scale cultivation and the tankage of food fermentation industry have been thrown aside etc. not only severe contamination everywhere environment has also greatly been wasted a large amount of nutrient resource (C, N, P, K, S and trace element).The present invention is intended to isolate the bacterial classification that a plant height produces γ-polyglutamic acid from soil, and this bacterial strain can be a main raw material with cow dung and msg meal, adds auxiliary material: wheat bran, Semen Maydis powder, analysis for soybean powder, citric acid, MgSO
47H
2O, MnSO
4H
2O produces γ-polyglutamic acid by solid fermentation, has advantages such as raw material sources are wide, cost is low, zymotechnique is simple, output is high, product extraction purifying is convenient, and good application prospects is arranged.
Three, summary of the invention
Technical problem
The object of the invention is to separate the bacterial classification that a plant height produces γ-polyglutamic acid from soil, utilizes solid waste and cheap agricultural byproducts to produce γ-polyglutamic acid by solid fermentation.
Technical scheme
A kind of bacterium YXY-C1 that can solid fermentation produces γ-polyglutamic acid, belong to Death Valley genus bacillus (Bacillus vallismortis), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 30th, 2009, culture presevation number is CGMCC NO.3478.Main biological characteristics is: Gram-positive G
+Cell shape is shaft-like, gives birth in the gemma, and hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, Mierocrystalline cellulose decompose feminine gender, gelatin liquefaction positive, the nitrate reduction positive, the indole test positive, can utilize Citrate trianion, glucose fermentation, wood sugar, sucrose produce sour aerogenesis.
This bacterial strain is preserved on the substratum after 30 ℃ of cultivations at solid, can be in 4 ℃ of short term storages; Simultaneously with seed liquor and 30% glycerine by-70 ℃ of following long-term frozen preservations behind 1: 1 volume ratio mixing, as original strain.Production need utilize after original strain switching activation with bacterial classification again.
With the method that described bacterium YXY-C1 solid fermentation is produced γ-polyglutamic acid, comprising: with cow dung and msg meal is the substratum main raw material, and adds auxiliary material: wheat bran, Semen Maydis powder, analysis for soybean powder, citric acid, MgSO
47H
2O and MnSO
4H
2O inserts the YXY-C1 seed liquor, produces γ-polyglutamic acid product by solid fermentation.Comprise:
1) seed liquor of YXY-C1 preparation
Bacterial strain YXY-C1 is inoculated in the seed liquor substratum, shake flask fermentation seeding liquid, fermentation condition is: pH growth scope 7.0-7.2,30 ℃ of culture temperature, stirring velocity is 180-200 rev/min, and fermentation time is 16-20 hour, thalline content in the fermented liquid or gemma amount 〉=1 * 10
9Individual/ml; Used seed liquor culture medium preparation method is (is example with preparation 1L substratum): take by weighing peptone 10 grams, and yeast extract paste 10 grams, sodium-chlor (NaCl) 10g, water are settled to lL, and pH value modulation 7.0-7.2 sterilized 20 minutes for 121 ℃.
2) preparation solid fermentation substratum
Solid fermentation substratum (20g) compound method is: cow dung 2.94g, msg meal 2.10g, dregs of beans 1.92g, wheat bran 0.8g, Semen Maydis powder 0.59g, citric acid 0.16g, sal epsom (MgSO
47H
2O) 0.04g, manganous sulfate (MnSO
4H
2O) 0.024g regulates pH to 7.0 with phosphoric acid buffer, and regulating water content is 60%, 121 ℃ of sterilization 20 minutes.
3) solid fermentation condition
Seed liquor is inoculated in the solid fermentation substratum with 5% inoculum size, stirs, place thermostat container to leave standstill cultivation, the solid fermentation temperature is 37 ℃, humidity remains on more than 60% in the thermostat container, stirs fermented substrate once every 12 hours, and fermentation time is 48 hours.
4) the extraction purifying of γ-polyglutamic acid
After the fermentation ends, add the 100ml deionized water in fermented substrate, 4 layers of filtered through gauze are got filtrate behind the vibration mixing.Centrifugal removal thalline of filtrate and impurity, i.e. acquisition contains the supernatant of γ-polyglutamic acid crude extract.
Add the dehydrated alcohol of 3 times of volume precoolings in the supernatant, staticly settled γ-polyglutamic acid 4 hours, centrifugal acquisition precipitation.To precipitate and use the equivalent deionized water dissolving, with insoluble contamination precipitation, supernatant is the sample that contains γ-polyglutamic acid.With this sample vacuum lyophilization, can obtain the solid phase prod of the γ-polyglutamic acid of purifying, and in-20 ℃ of preservations.
5) γ-polyglutamic acid determination of yield
With the γ-polyglutamic acid behind the purifying under vacuum condition, 110 ℃ with the HCl hydrolysis of 6mol/L 24 hours, hydrolyzed solution filters, filtrate is used the wherein content of free glutamic acid of Biochrom 30 Special Automatic amino acid analysis systems measurements after rotary evaporation is removed HCl, be the output that YXY-C1 bacterial strain solid fermentation is produced γ-polyglutamic acid.
6) γ-polyglutamic acid molecular weight determination
Adopt Sodium palmityl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method to measure the molecular weight of γ-polyglutamic acid that YXY-C1 bacterial strain solid fermentation produces, γ-polyglutamic acid is with A Lixinlan-8GX dye (Fig. 3).
Beneficial effect
A large amount of free wetting ability carboxyls are arranged in γ-polyglutamic acid molecule, can between intramolecule or molecule, form hydrogen bond, have high water-soluble and suction moisture retention, reach as high as 1: 3500 times.This high-hydroscopicity material is combined with soil, not only can improve granule, can also improve soil soil moisture conservation, preserve moisture, the nutrient preserving capability energy, playing a positive role aspect deserted mountain, bald mountain range, the desert transforming.According to Science and Technology Daily on August 19th, 2002, it is raw material with γ-polyglutamic acid that people such as former quick husband teach in department of agriculture of Kyushu University, developed the extremely strong natto resin of a kind of water-absorbent, with this resin plant seed is wrapped, in desert and the water-deficient area plantation that plant can not grow, can very fast germination, effect is very good.
The present invention filters out the wild strain YXY-C1 that a strain capable of high-efficiency produces γ-polyglutamic acid from soil; selecting the feces of livestock and poultry (cow dung) behind the large-scale cultivation and the tankage (msg meal) of food fermentation industry for use is main raw material; and add some cheap agricultural byproducts; as wheat bran; Semen Maydis powder; dregs of beans etc. are auxiliary material; produce γ-polyglutamic acid by solid fermentation; the novel method that solves feces of livestock and poultry and fermentation industry tankage environmental pollution and nutrient loss problem not only can be provided; and can produce γ-polyglutamic acid, turn waste into wealth.The selected bacterial strain YXY-C1 of this invention produces γ-polyglutamic acid stable performance, and solid fermentation process is simple, and it is low to consume energy, and output height, product reclaim simple efficient, so have very strong using value.
Four, description of drawings
Fig. 1 is the solid γ-polyglutamic acid of YXY-C1 strain liquid fermenting, abstracting and purifying.Be white or slightly yellowy floss.
Fig. 2 is the SDS-PAGE collection of illustrative plates that YXY-C1 bacterial strain solid fermentation is finished the product γ-polyglutamic acid that extracts purifying.Swimming lane 1 is a standard protein, and swimming lane 2 is a sample-loading buffer, and swimming lane 3 is the purified γ-polyglutamic acid of solid fermentation, and molecular weight is 130-300KD.
Fig. 3 is the relevant picture of YXY-Cl bacterial strain.1 is the gramstaining photo of YXY-C1 bacterial strain; 2 is the spore staining photo of YXY-C1 bacterial strain; 3 is the capsule stain photo of YXY-C1 bacterial strain; 4 is the flat-plate bacterial colony wire drawing photo of YXY-C1 bacterial strain
The biomaterial preservation
Bacterium YXY-C1 belongs to Death Valley genus bacillus (Bacillus vallismortis), is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 30th, 2009, and culture presevation number is CGMCCNO.3478.
Five, embodiment
(1) strains separation and evaluation
Substratum:
Solid selective medium (g/L): citric acid 10, L-glutamic acid 10, NH
4C16, K
2HPO
41, MgSO
47H
2O 0.5, FeCl
36H
2O 0.02, CaCl
20.2, MnSO
4H
2O 0.05, agar 20, and pH 7.2.
Seed liquor substratum (g/L): peptone 10, yeast extract paste 5, NaCl 10, and pH 7.0.
Liquid shaking bottle fermention medium (g/L): citric acid 13.5, L-glutamic acid 10, NH
4C16.8, glycerine 75, K
2HPO
41, MgSO
47H
2O 0.5, FeCl
36H
2O 0.02CaCl
20.2, MnSO
4H
2O 0.05, and pH 7.2.
Substratum (g/L) is preserved on the inclined-plane: peptone 10, and yeast extract paste 5, NaCl 10, agar 20, pH 7.0.
Strains separation:
Take by weighing and be collected near the vegetable garden soil sample 10g in Xuanwu District, Nanjing, Jiangsu Province, put into the triangular flask that 90ml sterilized water and granulated glass sphere are housed, the following 150 rev/mins of vibrations of room temperature boiling water bath 3-5 minute, make 10 after 30 minutes more successively
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6Different dilution soil supensions are coated on the solid selective medium at last.With flat board as for cultivating in 37 ℃ of incubators 1-2 days.It is bigger to select viscosity from flat board, and can the stringy bacterium colony, and streak inoculation is to the solid selective medium, up to obtaining pure culture.
Transfering loop picking one ring is dull and stereotyped single colony inoculation in seed culture medium, cultivated 16 hours for 30 ℃ following 170 rev/mins, seed liquor is inoculated into (1/5 liquid amount) in the liquid shaking bottle fermention medium by 5% inoculum size, 30 ℃ of following 170 rev/mins of shaking tables were cultivated after 48 hours, 10000 rev/mins of fermented liquids are after centrifugal 30 minutes, get supernatant, the dehydrated alcohol that adds the precooling of 3 times of volumes, standing over night, abandon supernatant after centrifugal 30 minutes for 12000 rev/mins, after the precipitate with deionized water dissolving, dialysed overnight, lyophilize obtains the solid primary extract.The primary extract deionized water dissolving, 12000 rev/mins centrifugal 10 minutes, on reset and add the 20mg/ml Proteinase K, the deionized water dialysed overnight, 12000 rev/mins centrifugal 10 minutes, get the supernatant liquor vacuum lyophilization, obtain the pure product of solid γ-PGA (Fig. 1), in-20 ℃ of preservations.
γ-PGA sample the 0.2g that gets purifying puts into the hydrolysis pipe, adds the HCl of 10mL 6mol/L, vacuumizes, seal after keeping 10min, and 110 ℃ of hydrolysis 24 hours, cooled and filtered is removed HCl with rotary evaporimeter again, elution three times, constant volume is to 10ml at last.Adopt the Special Automatic amino acid analysis of Biochrom 30 system that hydrolysate is analyzed, survey wherein free glutamic acid content.As calculated, the output of YXY-C1 bacterial strain in the liquid shaking bottle substratum can reach 18.4g/L.Cultivate the back proof through going down to posterity several times, the stable yield of YXY-C1 bacterial strain in the liquid shaking bottle substratum.
Finally choose the highest bacterial classification YXY-C1 of γ-polyglutamic acid output, the streak inoculation of pure culture bacterial classification is preserved on the substratum in solid, 4 ℃ of preservations, and the switching activation is once every other month; And with seed liquor and 30% glycerine by-70 ℃ of following prolonged preservation behind 1: 1 volume ratio mixing, as original strain.Production need utilize after original strain switching activation with bacterial classification again.
Strain identification:
By Physiology and biochemistry evaluation and 16S rDNA bacterial strain YXY-C1 being identified genus plants.Its dull and stereotyped characteristic is: bacterium colony is for circular, and the bacterium colony smooth surface is shinny, neat in edge, and center protrusion, colourless or slightly yellow, translucent to transparent, and viscosity is bigger; Main biological characteristics is Gram-positive G
+Cell shape is shaft-like, gives birth in the gemma, and hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, Mierocrystalline cellulose decompose feminine gender, gelatin liquefaction positive, the nitrate reduction positive, the indole test positive, can utilize Citrate trianion, glucose fermentation, wood sugar, sucrose produce sour aerogenesis; Be accredited as a strain Death Valley bacillus vallismortis through 16S rDNA.
(2) solid fermentation is produced γ-polyglutamic acid
The seed liquor preparation:
Seed liquor substratum (g/L): peptone 10, yeast extract paste 5, NaCl 10, and pH 7.0.
The single colony inoculation of transfering loop picking one ring activatory YXY-C1 was cultivated 16 hours for 30 ℃ following 170 rev/mins in the seed liquor substratum, behind sediments microscope inspection, determined that thalli morphology is single, and gemma form less, as the solid fermentation primary seed solution.Primary seed solution is inoculated in the seed liquor substratum by 5% inoculum size again, cultivated 16 hours for 30 ℃ following 170 rev/mins, behind the microscopy, standby as the solid fermentation secondary seed solution.
The solid fermentation substratum:
Solid fermentation carries out in the 250ml triangular flask, and the fermention medium Intake Quantity is 20g, and composition is: cow dung 2.94g, msg meal 2.10g, dregs of beans 1.92g, wheat bran 0.8g, Semen Maydis powder 0.59g, citric acid 0.16g, sal epsom (MgSO
47H
2O) 0.04g, manganous sulfate (MnSO
4H
2O) 0.024g regulates pH to 7.0 with phosphoric acid buffer, and regulating water content is 60%, 121 ℃ of sterilization 20 minutes.
The solid fermentation condition:
The solid fermentation secondary seed solution is inoculated in the solid fermentation substratum with 5% inoculum size, stirs.Place thermostat container to leave standstill cultivation triangular flask, the solid fermentation temperature is 37 ℃, and humidity remains on more than 60% in the thermostat container, thoroughly stirs fermented substrate once every 12 hours, to guarantee thalline evenly growth in the solid fermentation substratum, fermentation time is 48 hours.
γ-polyglutamic acid extracts purifying:
After the fermentation ends, in fermented substrate, add the 100ml deionized water, under the room temperature in shaking table 160 rev/mins of vibration mixings, suspension liquid is with 4 layers of filtered through gauze, 12000 rev/mins of centrifugal 30 minutes removal thalline of filtrate and impurity, i.e. acquisition contains the supernatant of γ-polyglutamic acid crude extract.The dehydrated alcohol that adds 3 times of volume precoolings in the supernatant staticly settled γ-polyglutamic acid 4 hours under 4 ℃, and 12000 rev/mins obtained precipitation in centrifugal 30 minutes.To precipitate and use the equivalent deionized water dissolving, 12000 rev/mins centrifugal 10 minutes with insoluble contamination precipitation, supernatant is the sample that contains γ-polyglutamic acid.With this sample vacuum lyophilization, can obtain the solid sample of the γ-polyglutamic acid of purifying, and in-20 ℃ of preservations.
The volume analysis of solid fermentation γ-polyglutamic acid:
γ-PGA sample the 0.2g that takes by weighing purifying puts into the hydrolysis pipe, the HCl that adds 10mL 6mol/L, vacuumize, keep 10 minutes after, pressurizing window, 110 ℃ of hydrolysis 24h, cooled and filtered is removed HCl, elution three times with rotary evaporimeter again, last constant volume is to 10ml, and each step all adopts ultrapure water to handle.Use the wherein content of free glutamic acid of Biochrom 30 Special Automatic amino acid analysis systems measurements after the hydrolyzed solution filtering and concentrating.Learn that the output of YXY-C1 bacterial strain γ-polyglutamic acid in this solid fermentation substratum is the 39g/kg substratum as calculated with after converting.
γ-polyglutamic acid molecular weight determination:
Adopt Sodium palmityl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method to measure the molecular weight of the γ-polyglutamic acid of YXY-C 1 bacterial strain solid fermentation production, the A Lixinlan 8GX dyeing (Fig. 2) of γ-polyglutamic acid, as seen in the scope of molecular weight distribution more than 130kD-300kD of the γ-polyglutamic acid of YXY-C1 bacterial strain solid fermentation production, belong to high molecular polymer.
Claims (7)
1. a solid fermentation is produced the bacterium YXY-C1 of γ-polyglutamic acid, belong to Death Valley genus bacillus (Bacillusvallismortis), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 30th, 2009, culture presevation number is CGMCC NO.3478, and main biological characteristics is: Gram-positive G
+Cell shape is shaft-like, gives birth in the gemma, and hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, Mierocrystalline cellulose decompose feminine gender, gelatin liquefaction positive, the nitrate reduction positive, the indole test positive, can utilize Citrate trianion, glucose fermentation, wood sugar, sucrose produce sour aerogenesis.
2. with the method for the described bacterium YXY-C1 of claim 1 solid fermentation production γ-polyglutamic acid, comprising: with cow dung and msg meal is main raw material, adds auxiliary material: wheat bran, Semen Maydis powder, dregs of beans, citric acid, MgS0
47H
20 and MnS0
4H
20, insert the YXY-C1 seed liquor, produce γ-polyglutamic acid by solid fermentation.
3. according to the method for the described production of claim 2 γ-polyglutamic acid, it is characterized in that:
1) the described bacterial classification YXY-C 1 of claim 1 is inoculated in the seed liquor substratum, shake flask fermentation seeding liquid, fermentation condition is: pH growth scope 7.0-7.2,30 ℃ of culture temperature, stirring velocity is 180-200 rev/min, fermentation time is 16-20 hour, thalline content in the fermented liquid or gemma amount 〉=1 * 10
9Individual/ml; Used seed liquor culture medium preparation method is, is example with preparation 1L substratum: take by weighing peptone 10 grams, and yeast extract paste 10 grams, sodium-chlor 10g, water is settled to 1L, and pH value modulation 7.0-7.2 sterilized 20 minutes for 121 ℃;
2) preparation solid fermentation substratum 20g, its method is: cow dung 2.94g, msg meal 2.10g, dregs of beans 1.92g, wheat bran 0.8g, Semen Maydis powder 0.59g, citric acid 0.16g, MgS0
47H
200.04g, MnS0
4H
200.024g, regulate pH to 7.0 with phosphoric acid buffer, regulating water content is 60%, 121 ℃ of sterilization 20 minutes;
3) seed liquor is inoculated in the solid fermentation substratum with 5% inoculum size, stirs, place thermostat container to leave standstill cultivation, the solid fermentation temperature is 37 ℃, humidity remains on more than 60% in the thermostat container, stirs fermented substrate once every 12 hours, and fermentation time is 48 hours.
4. according to the method for claim 2 or 3 described production γ-polyglutamic acids, it is characterized in that:
1) add the 100ml deionized water in the fermented substrate, 4 layers of filtered through gauze are got filtrate behind the shaking table vibration mixing, centrifugal removal thalline of filtrate and impurity, and supernatant is the product that contains γ-polyglutamic acid crude extract.
2) dehydrated alcohol of 3 times of volume precoolings of adding in the supernatant product that contains γ-polyglutamic acid crude extract, 4 ℃ staticly settled γ-polyglutamic acid 4 hours, centrifugal acquisition precipitation, precipitation equivalent deionized water dissolving, centrifugal with insoluble contamination precipitation, the supernatant vacuum lyophilization can obtain the solid phase prod of the γ-polyglutamic acid of purifying.
5. γ-polyglutamic acid product of obtaining of the described method of one of claim 2-4.
6. the application of the described γ of claim 5-polyglutamic acid product.
The described method of one of claim 2-4 obtain γ-polyglutamic acid product measuring method, comprising:
1) with the γ-polyglutamic acid behind the purifying under vacuum condition, 110 ℃ with the HCl hydrolysis of 6mol/L 24 hours;
2) hydrolyzed solution filters, and filtrate is used the wherein content of free glutamic acid of Biochrom 30 Special Automatic amino acid analysis systems measurements after rotary evaporation is removed HCl, be the output that YXY-C1 bacterial strain solid fermentation is produced γ-polyglutamic acid.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1718735A (en) * | 2004-07-06 | 2006-01-11 | 黑龙江省科学院应用微生物研究所 | Utilize the bacillus natto solid fermentation to produce polyglutamic acid and product application |
| CN101209350A (en) * | 2006-12-30 | 2008-07-02 | 中国人民解放军军事医学科学院毒物药物研究所 | Polyglutamate-medicament coupling compound with amino acid as communicating terminal |
-
2010
- 2010-08-20 CN CN2010102585172A patent/CN101948769B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1718735A (en) * | 2004-07-06 | 2006-01-11 | 黑龙江省科学院应用微生物研究所 | Utilize the bacillus natto solid fermentation to produce polyglutamic acid and product application |
| CN101209350A (en) * | 2006-12-30 | 2008-07-02 | 中国人民解放军军事医学科学院毒物药物研究所 | Polyglutamate-medicament coupling compound with amino acid as communicating terminal |
Non-Patent Citations (1)
| Title |
|---|
| 《微生物学通报》 20080920 冯静等 gamma-聚谷氨酸产生菌BLN-2的分离鉴定及固体发酵条件初探 1353-1358 5,7 第35卷, 第9期 2 * |
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| CN104762087B (en) * | 2015-01-21 | 2018-03-13 | 河海大学 | It is a kind of to be used to improve microorganism additive of beach saline-alkali soil texture and its preparation method and application |
| CN104987255A (en) * | 2015-08-07 | 2015-10-21 | 中国科学院合肥物质科学研究院 | Effect enhancing method of compound biobased fertilizer and fertilizer with enhanced effect |
| CN104987255B (en) * | 2015-08-07 | 2018-07-10 | 中国科学院合肥物质科学研究院 | A kind of compound bio base chemical fertilizer puies forward efficacious prescriptions method and carries effect chemical fertilizer |
| CN105837815A (en) * | 2016-05-17 | 2016-08-10 | 东莞理工学院 | Technology for efficiently extracting gama-polyglutamic acid from fermentation liquor |
| CN108102968A (en) * | 2018-01-16 | 2018-06-01 | 民勤县恒盛贸易有限公司 | A kind of wheat planting is with microorganism and preparation method thereof |
| CN111961604A (en) * | 2020-01-21 | 2020-11-20 | 吉林农业大学 | Fermentation culture solution for extracellular bacteriostatic protein produced by bacillus vallismortis SZ-4 and fermentation method thereof |
| CN111961604B (en) * | 2020-01-21 | 2021-09-28 | 吉林农业大学 | Fermentation culture solution for extracellular bacteriostatic protein produced by bacillus vallismortis SZ-4 and fermentation method thereof |
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