CN104788143B - A kind of utilization acid hydrolysis solution produces the method and product of biological organic fertilizer with decomposed manure - Google Patents
A kind of utilization acid hydrolysis solution produces the method and product of biological organic fertilizer with decomposed manure Download PDFInfo
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Abstract
The invention discloses the method and product that a kind of utilization acid hydrolysis solution and decomposed manure produce biological organic fertilizer.By acid hydrolysis solution with 0.2mL g‑1(dry weight, similarly hereinafter) adds decomposed manure and banked up naturally at room temperature 35 days, and daily turning once, treats that compost pH gos up to 56, access 0.05mL g‑1Function bacterium SQR9 or other aimed strains, daily turning 1 time are fermented 34 days.The biological organic fertilizer Promoting bacteria quantity produced is more than 1 × 108CFU g‑1, total nitrogen content is 1.5 2.5%, and total npk nutrient is that the 5 6%, content of organic matter is more than 30%, and simultaneously containing substantial amounts of free amino acid and polypeptide nutriment, production cost is low, growth-promoting effect is good, can be applied to cereal crops, vegetables etc..The technology greatly reduces the cost of biological organic fertilizer production, improves the market competitiveness of biological organic fertilizer, also to solve the problems, such as that animals died of illness provides support, mitigates its pollution to environment.
Description
Technical field
The invention belongs to microbial manure field, it is related to one kind and produces biological organic fertilizer with decomposed manure using acid hydrolysis solution
Method and product.
Background technology
Reform and open up to the outside world over more than 30 years, the ratio that China's animal husbandry accounts for the general production value of agriculture is increased to from the 25.67% of nineteen ninety
35.49% in 2008, animal husbandry gradually turns into the pillar industry of rural economy from household sideline production.The animal husbandry of China has been entered
Enter the large-scale cultivation stage, ten thousand, the even pig farm of hundreds of thousands head or the poultry farm of millions of plumages be found everywhere, it is ensured that Chinese people
Demand of the people to meat products.However, highly intensive aquaculture improves animal natural death rate, sudden epidemic disease is run into
Disease, mortality of animals is just higher.If these animals died of illness are without effective harmless treatment, environment can be caused by directly abandoning
Pollution, is obtained into market by criminal and then causes serious threat to the healthy and public safety of people.State Council, agriculture
The successively repeatedly dispatch of industry portion, starts the processing action of animals died of illness, however, there is presently no effective processing in science and technology
Approach, as governments at all levels' most thorny issue.
If animals died of illness thoroughly carries out harmless treatment not in time, the generation and propagation of animal epidemic will be caused, given
Aquaculture brings great threat.For example, China mainly has to the processing method of sick dead pig at present：It is buried, compost, burn etc. side
Method.It is buried to take a large amount of soils, and directly pollute the soil body, in some instances it may even be possible to polluted underground water；Compost is directly exposed due to the human body
In atmosphere, it is easy to cause pathogen atmosphere pollution；Although it is thorough to burn processing, consuming cost is big, and also very big
Waste of resource.
The present inventor in research before successfully by sick dead pig by sulfuric acid at high temperature acidolysis into amino-acid liquid,
The harm of pathogen is completely solved, and sick dead pig albumen is converted into free amino acid and polypeptide (number of patent application：
201410042218.3).It is higher yet with animals died of illness water content, after acidolysis the content of acid hydrolysis solution amino acid often compared with
It is low, it is impossible to be utilized as amino acid foliage fertilizer (amino acid content have to be larger than 10%).How the such acidolysis of substantial amounts of processing
Liquid turns into the key of processing animals died of illness problem.
Biological organic fertilizer refers to microorganisms with specific functions with main with plant and animal residues (such as feces of livestock and poultry, agricultural crop straw
Deng) it is that the class originated and be composited through harmless treatment, the organic materials become thoroughly decomposed has microbial manure and organic fertilizer efficiency concurrently
The fertilizer answered.Its substantive characteristics is containing specific function, shows the microorganism of certain fertilizer effect, the micro- life of these functions
The vital movement of thing is the key factor that biological organic fertilizer is better than other fertilizer.Biological organic fertilizer can produce various active after applying
Material dissolving soil in insoluble compound with increase soil fertility and be conducive to crops absorb nutrient；Produce antibacterial substance and life
Long regulator reduces the generation of crop soil-borne disease insect pest and promotes crop robust growth.But due to for cultivating the organic of Promoting bacteria
Carrier (soya-bean cake, rapeseed meal, the amino acid source such as feather meal) price is relatively expensive, causes bio-organic fertilizer product price higher, big face
Product popularization and application biological organic fertilizer is influenceed by certain, and therefore, the searching of cheap amino acid source organic carrier substitute turns into
Important topic.
After this patent is mixed using cheap acid hydrolysis solution with decomposed manure, access function bacterial strain carries out secondary solid
Fermentation generation novel biological fertilizer.It is not only to solve the problems, such as that animals died of illness provides support, mitigates its pollution to environment, and
And the cost of biological organic fertilizer production has been greatly reduced, improve the market competitiveness of biological organic fertilizer.
The content of the invention
Present invention aims at can not largely be handled and biological organic fertilizer production cost costliness and Promoting bacteria for acid hydrolysis solution
The problem of quantity cannot be ensured, it is the amino acid starting material in biological organic fertilizer formula to develop a kind of utilization acid hydrolysis solution, is developed
Formula and technique rich in functional microorganism and free amino acid and polypeptide nutriment biological organic fertilizer.
A kind of method that utilization acid hydrolysis solution produces biological organic fertilizer with decomposed manure, comprises the following steps：
1) fruits and vegetables Promoting bacteria is inoculated into fermentation culture, carries out liquid fermentation production, make to contain bacterium or bud in zymotic fluid
Spore amount is more than 1 × 109CFU mL-1Obtain fruits and vegetables growth-promoting fermented liquid；
2) by acid hydrolysis solution with 0.2~0.4mL g-1(dry weight, similarly hereinafter) is preferably with 0.2mL g-1Decomposed manure is added to carry out
Uniform mixing, stacking at room temperature 3-5 days, daily turning 1 time, pH can go up to 5-6；
3) by step 1) prepare fruits and vegetables growth-promoting fermented liquid with 0.05mL g-1Access step 2) prepare addition acid hydrolysis solution
And in the compost for the pH that gos up, daily turning 1 time, after fermentation 3-4 days, growth-promoting bacterial content can reach 1 × 108CFU g-1More than, most
The water content of microbial organic fertilizer is evaporated to less than 30% under conditions of temperature is no more than 60 DEG C afterwards, as refined biometric has
Machine fertilizer.
Wherein, described acid hydrolysis solution is obtained to domestic animal lean meat hydrolysis of dying of illness using sulfuric acid at 80~90 DEG C.
Described fruits and vegetables Promoting bacteria is selected from cucumber rhizosphere growth-promoting bacillus SQR9, and culture presevation number is CGMCC
NO.5808；The Antagonistic Fungi NJN-6 of continuous cropping banana Panama wilt disease is prevented and kill off, culture presevation number is CGMCC NO.3183；Prevent and kill off
The Antagonistic Fungi 021 of continuous cropping watermelon blight, culture presevation number is any one in CGMCC NO, 1544；It is preferred that cucumber rhizosphere
Growth-promoting bacillus SQR9, culture presevation number is CGMCCNO.5808；Prevent and kill off the Antagonistic Fungi of continuous cropping banana Panama wilt disease
NJN-6, culture presevation number is any one in CGMCC NO.3183；Most preferably fruits and vegetables Promoting bacteria is selected from cucumber rhizosphere growth-promoting
Bacillus SQR9, culture presevation number is CGMCC NO.5808.
Preferred as one kind of the present invention, the condition of the fermenting and producing of above-mentioned fruits and vegetables Promoting bacteria is：PH growth scopes naturally,
30~35 DEG C of cultivation temperature scope, mixing speed is 180~200rpm, and the fermentation middle and later periods forms gemma, makes to contain bacterium in zymotic fluid
Or gemma amount is more than 1 × 109CFUmL-1.The culture medium used that ferments PDA nutrient solutions preferably used, compound method is, to prepare
Exemplified by 1L culture mediums：It is cut into small pieces to be put into water after being peeled with 200g potatoes and boils, 30min is boiled after boiling, after filtering in filtrate
Plus 20g common sucroses, 1000ml is settled to, pH value is adjusted to 7.0-7.6,121 DEG C of sterilizing 20min.
Described acid hydrolysis solution preferably uses sulfuric acid of the concentration for 5~7mol/L at 80~90 DEG C to domestic animal lean meat water of dying of illness
The material ratio of 5~7h of solution, lean meat and the sulfuric acid of 5 described~7mol/L is 1:1.5~2.5.
Described acid hydrolysis solution main component：Total free amino acid content is 34.73g L-1, free amino acid, solubility are more
Peptide and soluble protein state total nitrogen (N) are 9.53g L-1。
Described decomposed manure is in Chicken dung organic fertilizer, rotted pig manure organic fertilizer, decomposed cow dung organic fertilizer
Any one or more；Decomposed manure can be made by oneself, also can purchase commercially available prod, do not influence its effect；It is preferred that chicken of becoming thoroughly decomposed
Excrement organic fertilizer or decomposed cow dung；The Chicken dung of Huinong bio tech ltd of further preferred Jiangsu Province production or Jiangsu Province
The rotted pig manure of Apollo bio tech ltd production.The Chicken dung of Huinong bio tech ltd of Jiangsu Province production
PH, water content, total carbon, total nitrogen (N), total phosphorus (P2O5), total potassium (K2O) content be respectively 8.34,28.37%, 14.48%,
1.84%th, 1.94% and 1.23%；It is the rotted pig manure pH of Apollo bio tech ltd of Jiangsu Province production, water content, total
Carbon, total nitrogen (N), total phosphorus (P2O5), total potassium (K2O) content is respectively 7.32,26.23%, 19.48%, 1.76%, 2.12% and
1.87%.
In the biological organic fertilizer prepared according to the method described in the present invention, the product function bacterium viable count be more than 1 ×
108CFU g-1, total nitrogen content is 1.5-2.5%, and total npk nutrient is 5-6%, and the content of organic matter is more than 30%, and its germination refers to
Number is more than 95%.
The beneficial effect present invention provides the formula that a kind of utilization acid hydrolysis solution develops growth-promoting biological organic fertilizer with decomposed manure
And technique.
The biological organic fertilizer produced using the invention, which is had, is rich in functional microorganism and free amino acid, and growth-promoting effect is good
Advantage, is adapted to the industrial crops planting area large area such as vegetables, fruit tree and uses.The present invention protects people for preserving the ecological environment
The people's is healthy, and the added value for improving agricultural product has great importance.
Product has the following advantages that compared with product in the market：
1) present invention successfully make use of acid hydrolysis solution, for processing die of illness domestic animal the problem of provide important support, become evil into
Treasured, protects environment.
2) product of the present invention significantly reduces the cost of material of biological organic fertilizer, promotes biological organic fertilizer to have very strong city
Field competitiveness.
3) acid hydrolysis solution has the amino acid of abundant dissolved organic carbon nitrogen and A wide selection of colours and designs, can provide very excellent to Promoting bacteria
The content of high efficient strain is more than 1 × 10 in growing environment more, the biological organic fertilizer developed8CFU g-1More than, apply soil
Advantage group can be formed after earth, and is survived in soil, function bacterium colonizing in soil and function is greatly improved
Ability.
4) the amino acid resource enriched in fertilizer can promote plant growth, and result of the test shows, be applied in vegetable sprout term
After this product, the growth of crop can be remarkably promoted.
Brief description of the drawings
Fig. 1 addition different proportion acid hydrolysis solution Chicken dung organic fertilizers are directly accessed Promoting bacteria SQR9 secondary fermentation number changes
Fig. 2 adds different proportion acid hydrolysis solution Chicken dung organic fertilizer secondary fermentation pH situations of change
Fig. 3 addition different proportion acid hydrolysis solution Chicken dungs pH accesses Promoting bacteria SQR9 secondary fermentation number changes after ging up
Fig. 4 additions different proportion acid hydrolysis solution rotted pig manure is directly accessed Promoting bacteria SQR9 secondary fermentation number changes
Fig. 5 adds different proportion acid hydrolysis solution rotted pig manure compost pH situations of change
Access Promoting bacteria SQR9 secondary fermentations quantity becomes after Fig. 6 addition different proportion acid hydrolysis solution rotted pig manure compost pH gos up
Change
Fig. 7 acid hydrolysis solutions are with 0.2ml g-1Add access function bacterium NJN-6 and 021 secondary fermentation number after Chicken dung pH gos up
Measure situation of change
Fig. 8 Chicken dungs organic fertilizer addition acid hydrolysis solution produces biological organic fertilizer cucumber growth-promoting figure
Fig. 9 Chicken dungs organic fertilizer addition acid hydrolysis solution produces biological organic fertilizer tomato growth-promoting figure
Figure 10 rotted pig manures organic fertilizer addition acid hydrolysis solution produces biological organic fertilizer cucumber growth-promoting figure (with the nutrient such as compareing)
Figure 11 rotted pig manures organic fertilizer addition acid hydrolysis solution produces biological organic fertilizer tomato growth-promoting figure (with the nutrient such as compareing)
Biological deposits information
SQR9, Classification And Nomenclature is bacillus amyloliquefaciens Bacillus amyloliquefaciens, is preserved in China micro-
Biological inoculum preservation administration committee common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3,
Institute of Microorganism, Academia Sinica, preservation date is on 2 27th, 2012, and preserving number is CGMCC NO.5808.
NJN-6, Classification And Nomenclature is bacillus subtilis Bacillus subtilis, is preserved in Chinese microorganism strain guarantor
Administration committee's common micro-organisms center is hidden, preservation address is Datun Road, Chaoyang District, Beijing City, Chinese Academy of Sciences's microbe research
Institute, preservation date is on July 9th, 2009, and preserving number is CGMCC NO.3183.
021, Classification And Nomenclature is Paenibacillus polymyxa Paenibacillus polymyxa, is preserved in China Microbiological bacterium
Preservation administration committee common micro-organisms center is planted, preservation address is No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Chinese section
Institute of microbiology of institute, preservation date is on November 23rd, 2005, and preserving number is CGMCC NO.1544.
Embodiment
1st, the acquisition of acid hydrolysis solution, Chicken dung and rotted pig manure
Using 5mol/L sulfuric acid, at 80 DEG C, with 1:1.5 (pork quality g：Sulfuric acid volume ml) material ratio to dying of illness
Lean pork carries out acidolysis 6h and obtains acid hydrolysis solution.The palliating degradation degree of lean pork is 99.63%.Acid hydrolysis solution composition：Total free amino acid contains
Measure as 34.73gL-1.Free amino acid, soluble polypeptide and soluble protein state total nitrogen (N) 9.53g L-1.Chicken dung is by river
Su Sheng Huinong bio tech ltd is provided, pH, water content, total carbon, total nitrogen (N), total phosphorus (P2O5), total potassium (K2O) content point
Wei 8.34,28.37%, 14.48%, 1.34%, 1.94% and 1.23%.Rotted pig manure is by Jiangsu Province's Apollo biotechnology
Co., Ltd provides, pH, water content, total carbon, total nitrogen (N), total phosphorus (P2O5), total potassium (K2O) content be respectively 7.32,
26.23%th, 19.48%, 1.76%, 2.12% and 1.87%.
2nd, the production of function bacteria agent
Promoting bacteria SQR9 or other fruits and vegetables Promoting bacterias NJN-6,021 are inoculated into PDA nutrient solutions, liquid fermentation is carried out
Produce, the condition of fermenting and producing is：PH growth scopes naturally, 30~35 DEG C of cultivation temperature scope, mixing speed is 180~
200rpm, the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount >=1 × 10 in zymotic fluid9CFU mL-1.PDA nutrient solutions used
Compound method is, exemplified by preparing 1L culture mediums：It is cut into small pieces to be put into water after being peeled with 200g potatoes and boils, is boiled after boiling
30min, adds 20g common sucroses after filtering in filtrate, be settled to 1000ml, and pH value is adjusted to 7.0-7.6,121 DEG C of sterilizings
20min。
3rd, solid fermentation generation biological organic fertilizer
The acquisition of 3.1 solid fermentations formula
The first step, by different proportion acid hydrolysis solution add Chicken dung (0.05,0.1,0.2,0.3,0.4mL g-1Represent respectively
5%th, 10%, 20%, 30%, 40%, be CK processing without amino acid), then it is directly accessed 0.05mL g-1(DW) starch is solved
Bacillus SQR9.Daily statistical function bacterium SQR9 quantity.As a result as shown in figure 1, function bacterium quantity addition acid hydrolysis solution it
Do not increase not only afterwards, there is decline on the contrary, analyze main reason is that compost pH is too low.Then first by different proportion acid hydrolysis solution
Access Chicken dung in (0.05,0.1,0.2,0.3,0.4mL g-15%, 10%, 20%, 30%, 40% is represented respectively, is not added
Plus acid hydrolysis solution be CK processing) carry out nature bank up, daily determine compost pH change.As a result as shown in Fig. 2 addition 0.3mL g-1
The compost of following acid hydrolysis solution is after 6 days solid state fermentations, and pH can rise to more than 5, can reach function bacterium SQR9 growth
It is required that, and 0.4mL g-1Addition pH is still too low.Go up after compost pH to 5-6, access 0.05mL g-1Solve starch gemma
Bacillus SQR9, daily measurement function bacterium SQR9 quantity.As a result it is as shown in Figure 3：CK, addition 0.05mL without acid hydrolysis solution
g-1, addition 0.1mL g-1And addition 0.4mL g-1Processing, function bacterium quantity is on a declining curve, only adds 0.2mL g-1
With 0.3mL g-1Processing function bacterium number amount rise, and 0.2mL g-1Addition is optimal, reaches 1.1 × 108CFU g-1。
Second step, by different proportion acid hydrolysis solution add rotted pig manure (0.15,0.2,0.25,0.3mL g-1Represent respectively
15%th, 20%, 25%, 30% processing, is CK processing without amino acid), then it is directly accessed 0.05mL g-1(DW) starch is solved
Bacillus SQR9, daily statistical function bacterium SQR9 quantity.As a result as shown in figure 4, function bacterium quantity is also in addition acid hydrolysis solution
Do not increase not only afterwards, have decline on the contrary, it is too low that analysis main cause lies also in compost pH.Then first by different proportion acid
Solve liquid access rotted pig manure in (0.15,0.2,0.25,0.3mL g-115%, 20%, 25%, 30% processing is represented respectively, is not added
Plus acid hydrolysis solution is CK processing) bank up naturally, the pH changes of compost are determined daily.As a result as shown in figure 5, all addition aminoacids solutions
Compost after 6 days solid state fermentations, pH can rise to more than 5, can reach function bacterium SQR9 growth requirement.Treat compost
PH gos up to 5-6, access 0.05mL g-1Bacillus amyloliquefaciens SQR9, daily measurement function bacterium SQR9 quantity.As a result such as
Shown in Fig. 6：CK, addition 0.25mL g without acid hydrolysis solution-1With addition 0.3mL g-1Processing, under function bacterium quantity is in
Drop trend, only adds 0.15mL g-1With 0.2mL g-1Processing function bacterium number amount rise, and 0.2mL g-1Addition is
Most preferably, 1.3 × 10 are reached8CFU g-1。
Pass through groping that first two steps are tested, it is known that acid hydrolysis solution 0.2mL g-1Addition being capable of the natural heaps of pH in universal organic fertilizer
It is more than 5 to put back into and rise to pH, and is adapted to function bacterium SQR9 fermentation.
3rd step, 0.2mL g-1 acid hydrolysis solutions are added into Chicken dung, after ging up through pH, access function bacterium NJN-6
With 021, as a result as shown in Figure 7：Function bacterium NJN-6 and 021 quantity also can be more than 1 × 108CFU g-1。
In summary, it is known that acid hydrolysis solution is added into after decomposed manure gos up naturally through pH and is adapted to various functions microorganism
Fermentation, have the great significance for popularization, and add 0.2mL g-1Acid hydrolysis solution is optimum ratio.
3.2 solid rafts fermentation generation finished product organic fertilizer
According to above-mentioned formula and technique, by acid hydrolysis solution with 0.2mL g-1It is respectively connected to decomposed manure uniformly to mix, daily
Once, Natural compost about 3-5 days to compost pH is 5-6, is respectively connected to 0.05mL g for turning-1Promoting bacteria SQR9, ferments 3-4 days.
Growth-promoting bacterium solution 50L is added in SOLID ORGANIC material per ton and carries out solid fermentation, daily turning 1 time makes solid fermentation temperature not surpass
50 DEG C are crossed, fermentation terminates after 3-4 days；Promoting bacteria quantity is more than 1 × 10 in novel biological fertilizer after fermentation ends8CFU g-1,
1 order of magnitude is got over more than decomposed manure.Biological organic fertilizer quality of organic matter is more than 30% than content, there is water content mass ratio
25-30%.The inexpensive growth-promoting biological organic fertilizer being developed into contains more than 1 × 108CFU g-1Promoting bacteria SQR9 above, full nitrogen
Content is 1.5-2.5%, and total npk nutrient is 5-6%, the content of organic matter more than 30%.Result above shows to develop formula
Maximized fermenting and producing novel biological fertilizer is fully able to technique, and the biological organic fertilizer is fully able to meet national mark
It is accurate.
4th, greenhouse growth-promoting is tested
The nutrient pot experiment such as or not 4.1 biological organic fertilizers on cucumber and tomato
Set two crops of cucumber and tomato altogether, each control seven of crop one processing is compareed as not fertilizer treatment (CK),
Processing is respectively common organic fertilizer Chicken dung processing (OF)；Chicken dung is directly accessed Promoting bacteria SQR9 compost treatments
(BIO0)；Chicken dung addition 0.05mL g-1Promoting bacteria SQR9 compost treatments (BIO5) are accessed after acid hydrolysis solution rise pH；Become thoroughly decomposed chicken
Excrement addition 0.1mL g-1Promoting bacteria SQR9 compost treatments (BIO10) are accessed after acid hydrolysis solution rise pH；Chicken dung adds 0.2mL
g-1Promoting bacteria SQR9 compost treatments (BIO20) are accessed after acid hydrolysis solution rise pH；Chicken dung addition 0.3mL g-1Acid hydrolysis solution gos up
Promoting bacteria SQR9 compost treatments (BIO30) are accessed after pH；Chicken dung addition 0.4mL g-1Growth-promoting is accessed after acid hydrolysis solution rise pH
Bacterium SQR9 compost treatments (BIO40)；9 basins of each processing, 2 ㎏ soil are filled per basin, the addition of fertilizer is 20g Kg-1.Fertilizer and soil
It is sufficiently mixed uniform, Routine Management.Cucumber and tomato growth index are determined after 35 days.As a result as Fig. 8, Fig. 9, table 1, table 2, table 3,
Table 4 and table 5.
Understand as shown in Table 1：Cucumber and tomato growth-promoting experiment in, BIO20 processing plant height be all significantly higher than CK, OF,
The processing such as BIO0, BIO5 and BIO10；Also above BIO30 and BIO40 processing, but difference is not notable.
Table 1：Influence of the different disposal to cucumber and tomato plant height
Understand as shown in Table 2：In the experiment of cucumber growth-promoting, its stem of the processing of the discarded pork acid hydrolysis solution fermentation of addition it is thick compared with CK,
OF processing differences are notable；In the experiment of tomato growth-promoting, the stem respectively handled is slightly not significantly different, and only the stem of blank is thick significantly less than it
He is handled.
Table 2：Influence of the different disposal to cucumber and tomato stem slightly
Understand as shown in Table 3：In cucumber, the experiment of tomato growth-promoting, add its number of blade of processing of aminoacids solution compared with CK, OF and
BIO0 processing is more, and significant difference.The number of blade of the processing of addition 20%, although with BIO5, BIO10, BIO30 and BIO40 processing
Without significant difference, but its number of blade average value is higher.
Table 3：Influence of the different disposal to cucumber and tomato leaf number
Understand as shown in Table 4：In the experiment of cucumber growth-promoting, there was no significant difference for the SPAD values of each processing chlorophyll.At BIO20
The SPAD of reason average value is handled higher than other；It is then the SPAD average value highests of BIO40 processing in the experiment of tomato growth-promoting, still
Its value and the difference that BIO20 is handled is not notable；
Table 4：Influence of the different disposal to cucumber and tomato chlorophyll
Understand as shown in Table 5：Cucumber, tomato growth-promoting experiment in, BIO20 processing fresh weight be significantly higher than CK, OF, BIO0,
The processing such as BIO5 and BIO10；Also above addition BIO30 and BIO40 processing, although difference is not notable.Result above shows, utilizes
Acid hydrolysis solution and Chicken dung production growth-promoting biological organic fertilizer are feasible, growth-promoting best results of the BIO20 processing for cucumber and tomato.
Table 5：Influence of the different disposal to cucumber and tomato fresh weight
4.2 biological organic fertilizers on cucumber and tomato etc. nutrient pot experiment
Two crops of cucumber and tomato are set altogether, and each control five of crop one processing is compareed as not fertilizer treatment
(ZCK), processing is respectively the Promoting bacteria SQR9 biological organic fertilizers (CBIO) that this seminar original rapeseed cake is fermented.Common organic fertilizer is rotten
Ripe swine manure treatment (ZOF)；Rotted pig manure is directly accessed Promoting bacteria SQR9 compost treatments (ZBIO)；Rotted pig manure addition 0.2mL g-1
Acid hydrolysis solution handles (ZAAOF)；Rotted pig manure addition 0.2mL g-1Promoting bacteria SQR9 compost treatments are accessed after acid hydrolysis solution rise pH
(ZAABIO)；9 basins of each processing, 3 ㎏ soil are filled per basin, the addition of fertilizer is 20g Kg-1.In addition to not fertilizer treatment, remaining
Processing chemical fertilizer polishing nutrient.Fertilizer is sufficiently mixed uniformly with soil, Routine Management.Cucumber is determined after 35 days and tomato growth refers to
Number.As a result such as Figure 10, Figure 11, table 6, table 7.
As shown in table 6：In the experiment of cucumber growth-promoting, ZBIO processing is compared to ZOF processing, in plant height, and stem is thick, the number of blade, leaf
It is not significantly different in terms of the biomass such as green element and fresh weight, it is seen that Promoting bacteria SQR9 is directly accessed in rotted pig manure not good
Effect.ZAAOF processing is compared in ZAABIO processing, except being not significantly different in terms of chlorophyll, and other aspects are all significantly excellent
In ZAAOF processing, it is known that access Promoting bacteria SQR9 can guarantee that Promoting bacteria on the basis of being added in rotted pig manure on amino acid
Quantity, so as to play the effect of growth-promoting plant growth.Two processing of ZAAOF and ZAABIO of addition amino acid, which are compared, is not added with ammonia
ZOF the and ZBIO processing of base acid, slightly and in terms of the number of blade improves in plant height, stem, shows in terms of chlorophyll and fresh weight
Write ground to improve, it is known that amino acid also has certain facilitation for the growth of crop.CBIO processing is compared in ZAABIO processing,
Significantly increase, at the thick aspect of stem without difference, and also dropped in terms of chlorophyll in terms of plant height, the number of blade and fresh weight
It is low, all in all, given birth to using acid hydrolysis solution and rotted pig manure fermenting organism organic fertilizer compared to using rapeseed cake and rotted pig manure fermentation
Organic fertilizer effect in terms of growth-promoting is more preferable.
Table 6：Different disposal influences on cucumber biomass
As shown in table 7：In the experiment of tomato growth-promoting, ZBIO processing is compared to ZOF processing, in plant height, and stem is thick, and the number of blade does not have
Significant difference, there is certain difference in terms of the biomass such as chlorophyll and fresh weight, but difference is few, it is seen that in rotted pig manure
Being directly accessed Promoting bacteria SQR9 does not have good effect.ZAAOF processing is compared in ZAABIO processing, although in plant height, and stem is thick, blade
It is not significantly different in terms of number and chlorophyll, but 14.4% is improved in terms of fresh weight, it is known that ammonia is added in rotted pig manure
Access Promoting bacteria SQR9 can guarantee that the quantity of Promoting bacteria on the basis of base acid is upper, so as to play the effect of growth-promoting plant growth.Add
Plus ZOF the and ZBIO processing for being not added with amino acid is compared in two processing of the ZAAOF and ZAABIO of amino acid, although in plant height and stem
Thick aspect is significantly increased without significantly difference in terms of the number of blade, chlorophyll and fresh weight, it is known that amino acid for
The growth of crop also has certain facilitation.CBIO processing is compared in ZAABIO processing, is had in terms of plant height, fresh weight and is significantly carried
Height, does not have difference stem is thick, in terms of the number of blade, chlorophyll, all in all, utilizes acidolysis amino acid and rotted pig manure fermenting organism
Organic fertilizer is compared to using rapeseed cake and rotted pig manure fermenting organism organic fertilizer, effect is more preferable in terms of growth-promoting.
Table 7：Different disposal influences on tomato biological amount
Solid fermentation experiment shows that the formula and technique of this research can remarkably promote the solid fermentation of function bacterium, increase
The nutrient of the quantity of Promoting bacteria and fertilizer in fertilizer；Results from pot experiment test integrally shows, the biological organic fertilizer energy that this patent is developed
The growth for examination plant is enough remarkably promoted, and the cost of bio-organic fertilizer special formula can be significantly reduced, while being also solution
Animals died of illness problem provides support, mitigates its pollution to environment, therefore, the biological organic fertilizer formula and work of this patent research and development
Skill has very strong practicality.
Claims (8)
1. a kind of method that utilization acid hydrolysis solution produces biological organic fertilizer with decomposed manure, it is characterised in that comprise the following steps：
1) fruits and vegetables Promoting bacteria is inoculated into fermentation culture, carries out liquid fermentation production, make to contain bacterium or gemma amount in zymotic fluid
More than 1 × 109 CFU mL-1Obtain fruits and vegetables growth-promoting fermented liquid；Described fruits and vegetables Promoting bacteria is selected from cucumber rhizosphere growth-promoting gemma bar
Bacterium SQR9, culture presevation number is CGMCC NO.5808；
2) by acid hydrolysis solution 0.2mL g in terms of dry weight-1Add decomposed manure uniformly to be mixed, stacking at room temperature 3-5 days is turned over daily
Heap 1 time, pH can go up to 5-6；
3) by step 1）The fruits and vegetables growth-promoting fermented liquid of preparation is with 0.05 mL g-1Access step 2）The addition acid hydrolysis solution of preparation is simultaneously
In rise pH compost, daily turning 1 time, after fermentation 3-4 days, growth-promoting bacterial content can reach 1 × 108 CFU g-1More than, finally
The water content of microbial organic fertilizer is evaporated to less than 30% under conditions of temperature is no more than 60 DEG C, as refined biometric is organic
Fertilizer；
Wherein, described acid hydrolysis solution is obtained to domestic animal lean meat hydrolysis of dying of illness using sulfuric acid at 80 ~ 90 DEG C.
2. according to the method described in claim 1, it is characterised in that described acid hydrolysis solution is the sulphur that concentration is 5 ~ 7mol/L
Acid hydrolyzes 5 ~ 7h at 80 ~ 105 DEG C to domestic animal lean meat of dying of illness, and the material ratio of lean meat and 5 described ~ 7mol/L sulfuric acid is 1:
1.5~2.5。
3. according to the method described in claim 1, it is characterised in that described decomposed manure is selected from Chicken dung organic fertilizer, corruption
Any one or more in ripe Swine manure, decomposed cow dung organic fertilizer.
4. method according to claim 3, it is characterised in that described decomposed manure is preferably that Jiangsu Province Huinong is biological
The Chicken dung of Science and Technology Ltd.'s production or the rotted pig manure of Apollo bio tech ltd of Jiangsu Province production.
5. the biological organic fertilizer prepared according to the method any one of claim 1 ~ 4, it is characterised in that work(in the product
Energy bacterium viable count is more than 1 × 108 CFU g-1, total nitrogen content is 1.5-2.5%, and total npk nutrient is 5-6%, the content of organic matter
More than 30%, its germination index is more than 95%.
6. the application of biological organic fertilizer prepared by the method any one of claim 1 ~ 4 in fruits and vegetables growth is promoted.
7. application according to claim 6, it is characterised in that described biological organic fertilizer answering in vegetable growth is promoted
With.
8. application according to claim 7, it is characterised in that described biological organic fertilizer is promoting cucumber and/or tomato life
Application in length.
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| CN108118009B (en) * | 2017-12-04 | 2021-06-08 | 南京农业大学 | Method for producing bio-fertilizer synergist by using tobacco foam and application |
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| CN110041138A (en) * | 2019-05-27 | 2019-07-23 | 南京农业大学 | A kind of method and product using acidolysis amino acid and biogas residue production biological organic fertilizer |
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| CN112745151A (en) * | 2021-01-11 | 2021-05-04 | 南京农业大学 | Composite biological organic fertilizer containing bacillus and trichoderma and preparation method thereof |
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