CN108118009B

CN108118009B - Method for producing bio-fertilizer synergist by using tobacco foam and application

Info

Publication number: CN108118009B
Application number: CN201711261147.6A
Authority: CN
Inventors: 张瑞福; 岳政府; 王伟; 刘凯鸣; 浦子晔; 翟欣奕; 沈其荣
Original assignee: Nanjing Agricultural University
Current assignee: Nanjing Agricultural University
Priority date: 2017-12-04
Filing date: 2017-12-04
Publication date: 2021-06-08
Anticipated expiration: 2037-12-04
Also published as: CN108118009A

Abstract

The invention discloses a method for producing a biological fertilizer synergist by using tobacco waste and application thereof. The smoke-foam anoxic fermentation liquor can be used as a synergist of microbial fertilizer or used in combination with a liquid microbial inoculum, and the diluent is applied in soil, so that the rhizosphere colonization quantity of growth-promoting bacteria can be increased, and the growth-promoting effect is good. The biological fertilizer synergist produced by the anoxic fermentation of the tobacco foam is rich in various organic acids, the pH value is weak acid, and the total organic acid content is high. The production process for producing the biological fertilizer synergist by using the tobacco waste is simple, the equipment requirement is low, the cost is low, and the tobacco waste resource can be effectively utilized.

Description

Method for producing bio-fertilizer synergist by using tobacco foam and application

Technical Field

The invention belongs to the field of waste resource utilization, and particularly relates to a method for producing a biological fertilizer by using tobacco waste and application thereof.

Background

The microbial fertilizer is prepared by adding functional microorganisms on the basis of decomposed organic fertilizers, the organic fertilizers can not only bring fertilizer efficiency to plants, but also serve as carriers for life activities of the functional microorganisms, and nutrients which are difficult to utilize in soil and fertilizer efficiency can be activated through the life activities and metabolites of the microorganisms, so that the fertilizer efficiency is further improved. Researches find that the interaction relationship exists between plants and functional microorganisms, and on one hand, the functional microorganisms provide nutrients for root systems, promote absorption and resist pathogenic microorganisms. On the other hand, functional microorganisms can also utilize root exudates as nutrients for reproduction and metabolic activities. Compont et al propose that effective colonization of functional microorganisms at the plant rhizosphere is an important prerequisite for their growth-promoting and biocontrol functions; bais et al also propose that efficacy is only exerted when functional microorganisms accumulate to a certain extent; de Weet et al found that one of the key factors affecting the colonization of functional microorganisms is their chemotaxis to root exudates, and that roots release various chemical components into the surrounding growth substrate during their growth, i.e., root exudates. At present, the research on the aspect is relatively deep, the interaction between the arbuscular mycorrhizal fungi and plants is realized, the root secretion of the plants such as corn, tomato and the like contains strigolactone, and Gomez-Roldan and the like find that the strigolactone can promote the colonization of the arbuscular mycorrhizal fungi at the plant rhizosphere; in addition, researches of Rudrappa and the like find that L-malic acid with proper dose can effectively attract rhizosphere colonization of rhizosphere growth-promoting bacteria (PGPR); munoz Aguilar et al have shown that rhizobia has chemotactic reaction on part of phenolic substances; liu and the like find that plant root secretion regulates the interaction between a cucumber root system and a rhizosphere microorganism SQR9, rhizosphere colonization is enhanced, after the cucumber root system is infected by Fusarium Oxysporum (FOC), the content of citric acid and fumaric acid in the root secretion is obviously increased, the citric acid can induce chemotaxis of PGPR, and the fumaric acid can stimulate the formation of a biofilm, so that the colonization of the PGPR at the rhizosphere is improved. The researches show that the functional microorganisms have obvious chemotactic effect on root exudates, particularly organic acid substances in the root exudates, so that the colonization amount of the functional microorganisms in plant rhizosphere is increased.

Anaerobic fermentation refers to the joint degradation of organic matters by facultative anaerobes and obligate anaerobes under the anaerobic condition, and finally, gases such as carbon dioxide, methane and the like can be generated. According to the three-stage theory, anaerobic fermentation is divided into three stages of hydrolysis, acid production and methane production. The hydrolysis stage, under the action of hydrolysis and fermentation bacteria, hydrolyzing macromolecular substances of carbohydrates, proteins and fats into micromolecular substances such as monosaccharides, amino acids, fatty acids, glycerol and the like; in the acid production stage, under the action of hydrogen-producing acetogenic bacteria, products in the hydrolysis stage are further decomposed into hydrogen, carbon dioxide and acetic acid; the methanogenic stage is the process of converting hydrogen and carbon dioxide to methane, and decarboxylating acetic acid to produce methane, through the action of two methanogenic bacteria. The three components interact with each other and influence each other. At present, most of researches on anaerobic fermentation acid production are carried out on substances containing rich organic matters, such as municipal sludge and kitchen waste, and most of researches are aimed at acid production process optimization, namely Chuawiana and the like indicate that higher temperature is not only beneficial to dissolving organic matters in the sludge hydrolytic fermentation process, but also can greatly promote the generation of intermediate organic acid, but also can inhibit the growth of methanobacteria. Researches such as Chenxingchun and the like find that compared with an alkaline environment, the acid production is more stable when the pH value is 6.5, the acid production amount is higher, but the alkaline condition is more suitable for producing acetic acid; berger et al studied the effect of C/N ratio conditions on key enzymes and acid production types by modeling; zhangyujing and other researches find that when the pH value is 6.0, the kitchen waste has the best hydrolytic acidification effect and generates more organic acid than other pH values; at pH 6.0, the concentration of Volatile Fatty Acid (VFA) and the acid yield per volatile matter (VS) reached maximum values at 68h, which were 40.89g/L and 0.328g of VFA/gVS, respectively, which were 8 times higher than those without pH control, and the pH affected the composition of the fermentation product significantly. It is noted that during the acidogenic stage, short-chain organic acids and some small molecular alcohols and ketones are produced, and studies have shown that organic acids such as malic acid (rudrapa et al, 2007) promote the recruitment of PGPR, and flavonoids in soybean root exudates attract beneficial microorganisms, increasing colonization (Morris et al, 1998). Therefore, the research on the influence of small molecular substances generated in the acid production stage on the chemotactic colonization of functional microorganisms is of great significance, and the research on the influence is very little.

The tobacco waste is a part of tobacco stem plant excluding tobacco leaves having a baking value, and includes waste tobacco leaves, cut tobacco, shredded tobacco pieces, tobacco stems, tobacco shreds, and the like. According to investigation, the annual total amount of tobacco waste in China is 90-100 million tons, which accounts for about 25% of the total yield, but the tobacco waste is not effectively utilized, most of the tobacco waste is discarded, resources are wasted, and the environment is polluted. At present, researches such as rotten composting and Zhang Xiang which are the most studied on tobacco waste show that after tobacco leftovers are biologically fermented, organic fertilizer and organic-inorganic compound fertilizer are prepared and applied to fields, cucumber and tomato yield increases by 12.0% and 15.9% respectively per hectare, the quality is improved to different degrees, 1/3 is reduced in the fertilizer, the organic fertilizer is applied, wheat and corn yield increases by 14.9% and 11.9% respectively compared with the control, the difference is obvious, and the contents of protein, starch and amino acid in grains are improved. The method comprises the steps of mechanically cutting tobacco field wastes by Chengang and the like, adding a tobacco fast-rotting agent to perform stacking fermentation, successfully producing 3000 tons of organic fertilizer, obtaining two commercial series products of powdery fertilizer and granular fertilizer, wherein the net yield is 101.1 ten thousand yuan, and the anthracnose, red star disease incidence, floral leaf disease incidence and two black disease incidence of the treated field tobacco are reduced to different degrees. The tobacco contains more than 3000 chemical compounds such as nicotine and solanesol, and most of the chemical compounds can be used as signal substances to participate in information regulation and control of soil microorganisms and interacting plants. At present, few researches on the production of the bio-fertilizer synergist by the anoxic fermentation of the smoke waste are needed, and further researches are needed.

Disclosure of Invention

Aiming at the conditions that the utilization rate of the existing tobacco foam solid waste is low and the field effect of the microbial fertilizer is unstable, the invention develops and develops the anoxic fermentation liquid compound which can efficiently utilize the tobacco foam waste and can enhance the rhizosphere colonization quantity of the plant growth-promoting bacteria so as to enhance the growth-promoting effect on crops.

It is another object of the present invention to provide a method for producing the liquid composite.

The purpose of the invention can be realized by the following technical scheme:

a liquid biofertilizer synergist is prepared from smoke through anoxic fermentation.

The tobacco foam is from discarded leftovers of a cigarette factory, and is sieved after being dried in the air.

The anaerobic fermentation is implemented by inoculating domesticated river bottom sludge by using tobacco waste as a fermentation substrate by using a tobacco foam anaerobic fermentation device, wherein the production condition of the anaerobic fermentation is that the initial pH is 5-6, the inoculation amount of the domesticated river bottom sludge is 10-15% of the fermentation substrate (mass ratio of materials, the same below), the final water content is 85-90%, and the fermentation is carried out for 140-150 h at 35-40 ℃.

The domestication method of the domesticated river bottom sludge used by the invention comprises the following steps: inoculating fresh river bottom sludge into the tobacco foam waste according to the inoculation amount of 20% by mass, wherein the final water content is 85%, the fermentation temperature is 37 ℃, the initial pH is 6.0, fermenting is carried out for 4 days, the fermentation product is used as an inoculum, the tobacco foam waste is used as a substrate, and then carrying out secondary fermentation, and the fermentation product obtained by the secondary fermentation is domesticated river bottom sludge.

The smoke foam anoxic fermentation device used by the invention is purchased from the market according to the smoke foam anoxic fermentation requirement (manufacturers: Sichuan cattle glass instrument Co., Ltd., Nantong Baisitai laboratory instruments Co., Ltd.; trade names: brown glass bottle and two-way bottle cap; model number: 1L and inner diameter: 4 cm).

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a production method for preparing a liquid biofertilizer synergist through anoxic fermentation of tobacco foam, which has the advantages of simple production process and low equipment requirement, and the prepared liquid compound has a good colonization promoting effect on functional strains, can be used as a synergist of a microbial fertilizer or used in combination with a liquid microbial inoculum, can efficiently utilize tobacco foam resources in a resource manner, and has important significance for protecting ecological environment and human health.

The liquid biofertilizer synergist prepared by the anaerobic fermentation of tobacco foam contains more than 3.0mg/mL of total volatile fatty acids, chemotactic experiments of diluent used for Bacillus amyloliquefaciens SQR9 show that SQR9 has obvious chemotactic effects on the diluent, and pot experiments show that the diluent can obviously enhance the colonization amount of SQR9 at the corn rhizosphere and has an obvious growth promoting effect.

Biological preservation information:

SQR9, classified name is Bacillus amyloliquefaciens, and is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of Xilu No. 1 of Beijing Korean Zhongyang district, the microbiological research institute of Chinese academy of sciences, the preservation date is 2012, 2 and 27 days, and the preservation number is CGMCC NO. 5808.

Drawings

FIG. 1 Effect of initial pH on total volatile fatty acid production by anoxic fermentation of tobacco froth.

FIG. 2 effect of inoculum size on total volatile fatty acid production by anaerobic fermentation of tobacco foam.

FIG. 3 the effect of moisture content on the total amount of volatile fatty acids produced by anoxic fermentation of tobacco froth.

FIG. 4 effect of fermentation temperature on total amount of volatile fatty acids produced by anaerobic fermentation of tobacco foam.

FIG. 5 effect of fermentation time on total amount of volatile fatty acids produced by anaerobic fermentation of tobacco foam.

FIG. 6 is a graph showing chemotactic effect of SQR9 on dilutions of different fold.

FIG. 7 shows the effect of dilution of the anaerobic fermentation broth of tobacco foam on the amount of SQR9 colonized at the rhizosphere and the growth promoting effect.

Detailed Description

Example 1

1. Obtaining of smoke

Provided by a certain cigarette factory.

2. Study on acid production conditions of anoxic fermentation

1) Initial pH: setting initial pH values (5.0, 6.0, 7.0 and 8.0) of different gradients, inoculating 10% (mass ratio, the same below), fermenting at 35 deg.C for 4 days, performing solid-liquid separation by suction filtration, and measuring total amount of volatile fatty acid in the liquid.

As a result: as can be seen from FIG. 1, the concentration of the total amount of volatile fatty acids gradually decreased between the initial pH of 5.0 and 7.0, and the concentration of the total amount of volatile fatty acids was the highest at the initial pH of 5.0 and reached 3.41 mg/mL. The optimal initial pH of the fermentation was determined to be 5.0.

2) And inoculation amount: setting different inoculum sizes (5%, 10%, 15% and 20%), initial pH 6.0, water content 85%, fermenting at 35 deg.C for 4 days, performing solid-liquid separation by suction filtration, and determining total amount of volatile fatty acid in the liquid.

As a result: as can be seen from FIG. 2, when the inoculation amount is 15% (mass ratio), the total amount of volatile fatty acids is the highest, and reaches 5.95mg/mL, and therefore, the inoculation amount of 15% is determined as the optimum inoculation amount.

3) Water content: setting different water contents (80%, 85%, 90% and 95%), initial pH 6.0, and inoculum size 10% (mass ratio), fermenting at 35 deg.C for 4 days, performing solid-liquid separation by suction filtration, and determining total amount of volatile fatty acid in the liquid.

As a result: as can be seen from fig. 3, the total amount of volatile fatty acids was the highest at a moisture content of 80%, and 85% was determined as the optimum moisture content, considering the stability of fermentation and economic cost, with no significant difference between 85% and 80%.

4) And fermentation temperature: setting different fermentation temperatures (30 ℃, 35 ℃, 40 ℃ and 45 ℃), setting the initial pH to be 6.0, the inoculation amount to be 10% (mass ratio, the same below) and the water content to be 85%, fermenting for 4 days, carrying out solid-liquid separation by suction filtration, and measuring the total amount of volatile fatty acid in the liquid.

As a result: as can be seen from FIG. 4, when the temperature was 35 ℃, the total amount of volatile fatty acids was the highest, reaching 3.66mg/mL, and thus 35 ℃ was determined as the optimum fermentation temperature.

5) And fermentation time: setting different fermentation time (sampling once every 12h, period is 7 days), initial pH is 6.0, inoculum size is 10% (mass ratio, the same below), water content is 85%, fermentation temperature is 35 ℃, sampling once every 12h, performing solid-liquid separation by suction filtration, and determining total volatile fatty acid content in liquid.

As a result: as can be seen from FIG. 5, when the fermentation time is 144h, the total amount of volatile fatty acids is the highest and reaches 3.15mg/mL, so that the 144h optimal fermentation time is determined.

In summary, the optimal anaerobic fermentation conditions are: initial pH of 5.0, inoculum size of 15%, water content of 85%, fermentation temperature of 35 deg.C, and fermentation time of 144 h. The total amount of volatile fatty acids reached 7.12 mg/mL.

3. Research on chemotactic movement of fermentation broth dilution by SQR9

The chemotactic effect of SQR9 on each treatment solution was investigated by a plate chemotaxis experiment, and 7 treatments were set in total:

1) chemotaxis buffer (negative control); 2)50 μ M citric acid (positive control); 3) smoke-oxygen-deficient fermentation stock solution; 4) diluting the smoke foam anoxic fermentation stock solution by 10 times; 5) diluting the smoke foam anoxic fermentation stock solution by 100 times; 6) diluting the smoke foam anoxic fermentation stock solution by 1000 times; 7) the smoke foam anoxic fermentation stock solution is diluted by 10000 times. After 3 times of each treatment, 10 μ l of each treatment solution is dripped into the middle position of the culture dish, then the culture dish is kept still at room temperature for 30min, and the formation situation of chemotactic circles in the culture dish is observed, and as a result, as shown in fig. 6, compared with a control, the SQR9 shows chemotactic responses of different degrees to 10 times, 100 times, 1000 times and 10000 times of dilution of the smoky froth anoxic fermentation broth, wherein the chemotactic circles diluted by 100 times and 1000 times are larger, namely the SQR9 has a remarkable chemotactic effect on 100 times and 1000 times of dilution of the smoky froth anoxic fermentation broth.

4. Influence of dilution of smoke-foam anoxic fermentation liquor on SQR9 in rhizosphere colonization amount and growth promotion effect

The SQR9 bacterial suspension is prepared by the following steps: the SQR9 strain preserved in-80 deg.C glycerin tube is streaked and activated on LB solid culture medium plate, and cultured in 37 deg.C incubator for 12 hr. Picking single colony in 3mL liquid LB tube, 37 deg.C, 170r min^-1The culture solution was shake-cultured for 10 hours to obtain a seed solution. The seed solution was transferred to a 250mL Erlenmeyer flask containing 100mL liquid LB medium at 1% (v/v) inoculum size at 37 ℃ for 170r min^-1Culturing to mid-log phase (OD)₆₀₀1.0), collecting the thalli by centrifugation at 25 ℃, discarding the supernatant, washing the thalli with sterilized distilled water for 3 times, and then resuspending the thalli in an equal volume for later use.

The culture medium is a mixture of soil (yellow brown soil) and quartz sand, wherein the ratio of soil: and 7:3 of quartz sand. The cultured crop is corn (Zhenuo No. 2). The influence of dilution liquid of the smoke-foam anoxic fermentation liquor on the colonization quantity of the SQR9 at the rhizosphere is researched by adopting a Real-timePCR technology, the corresponding growth promotion effect is analyzed by measuring 3 indexes of the plant height, the fresh weight and the dry weight of the overground part of the corn, and the experiment sets 7 treatments: 1) blank: without addingSQR9 and a tobacco foam broth; 2) y1000: no SQR9 is added, and only 1000 times of dilution liquid of the tobacco foam fermentation liquid is added; 3) y1000+ SQR9 (I): adding the inactivated SQR9, and applying a 1000-time dilution of the tobacco foam fermentation liquid; 4) water + SQR 9: adding SQR9, and applying an equal volume of clear water; 5) y1000+ SQR 9: adding SQR9, and applying 1000 times of dilution liquid of the tobacco foam fermentation liquid; 6) y2000+ SQR 9: adding SQR9, and adding 2000 times of dilution liquid of the tobacco foam fermentation liquid; 7) y5000+ SQR 9: SQR9 was added and 5000-fold dilutions of the smoke broth were applied. And (3) setting 20 times of repetition for each treatment, culturing the corns to a 2-leaf stage by using a seedling culture medium in a sterile environment, selecting corn seedlings with consistent growth vigor, transplanting the corn seedlings into culture bowls, and loading 1.5kg of culture medium in each bowl, wherein each bowl is provided with one seedling. Before seedling transplanting, spraying bacteria on culture medium in each pot according to treatment to make SQR9 in each pot have final concentration of 1 × 10⁷CFUs·g^-1And (3) culturing the substrate, and uniformly spraying 50mL of each dilution of the tobacco foam fermentation liquor or 50mL of clear water on each pot of the culture substrate. The duration of the whole pot experiment is 28 days, and 3 times of sampling are carried out in the period, namely 7 days after seedling transplantation, 14 days after seedling transplantation and 28 days after seedling transplantation. Extracting corn rhizosphere soil DNA by using DNeasy PowerSoil Kit, wherein the specific primer of SQR9 is 2F: 5'-ATAGCAAGAGCGAGGCAGAAGT-3'; 5'-CAGAGGAATCATCAACACCAACAGT-3', model number of Real-Time PCR instrument is StepOnePlus, and reaction system is as follows: SYBR Premix Ex Taq^TMII 10. mu.L, PCR Forward Primer (10. mu.M) 0.4. mu.L, PCR Reverse Primer (10. mu.M) 0.4. mu.L, ROX Reference Dye 0.4. mu. L, DNA template 2. mu. L, ddH₂O6.8. mu.L, 20. mu.L in total. A two-step PCR amplification procedure was used: stage 1: 30 seconds at 95 ℃; stage 2: reps40, 95 ℃ for 5 seconds, 60 ℃ for 34 seconds. Association Stage: 95 ℃ for 15 seconds, 60 ℃ for 1 minute, 95 ℃ for 15 seconds. The Real-time PCR results are shown in FIG. 7, compared with the control group Water + SQR9, the SQR9DNA copy number processed by Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 is improved no matter 7 days after seedling transplantation, 14 days after seedling transplantation, or 28 days after seedling transplantation, and the Y1000+ SQR9 and Y2000+ SQR9 processing groups sampled at other times except Y1000+ SQR9 at 7 days after seedling transplantation reach significant levels, which shows that the colonization of Bacillus amyloliquefaciens R9 at the rhizosphere of corn can be improved by applying 1000 times of dilution, 2000 times of dilution and 5000 times of dilution, wherein the 1000 times of dilution, and the SQR9 at the anoxic time of the smoke foam fermentation liquid,The effect of the 2000 times of diluent is obvious; as for the dilution multiple, the SQR9DNA copy values are Y2000+ SQR9, Y1000+ SQR9 and Y5000+ SQR9 from high to low, which shows that the effect of promoting the colonization of the SQR9 at the corn rhizosphere is best when 2000 times of dilution of the smoke anoxic fermentation liquor is applied. The statistical determination of the height of the corn plants, the fresh weight of the overground part and the dry weight of the overground part of each sample is carried out respectively, the results are shown in table 1, and the weight of the seedlings is measured 7 days after transplanting: compared with Blank, the plant heights of Y1000, Y1000+ SQR (I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 are respectively increased by 8.8%, 10.3%, 13.2%, 16.4%, 25.8% and 14.4%, which indicates that the tobacco foam fermentation liquor has certain nutrient effect, and the SQR9 thalli also contributes to partial growth promotion effect, in addition, the differences of Y1000+ SQR9 and Y2000+ SQR9 are obvious, wherein the treatment effect of Y2000+ SQR9 is optimal; the fresh weight of the overground part is respectively increased by 9.0%, 9.1%, 61.7%, 78.9%, 128.1% and 98.3%, which shows that the tobacco foam fermentation liquor has certain nutrient function, the organic matter of the SQR9 thallus also contributes to partial growth promotion function, in addition, the differences of the Water + SQR9, the Y1000+ SQR9, the Y2000+ SQR9 and the Y5000+ SQR9 are obvious, and the Y2000+ SQR9 has the best effect; the dry weight of the aerial parts increased by 29.6%, 38.9%, 97.8%, 125.9%, 74.1%, respectively. The tobacco foam fermentation liquid has certain nutrient effect, the self organic matter of the SQR9 thallus also contributes to partial growth promotion effect, in addition, the Y1000+ SQR9 and the Y2000+ SQR9 have obvious difference, and the Y2000+ SQR9 has the best effect. In general, nutrients of the smoke foam fermentation liquor can play a certain role in growth promotion, and organic matters of the SQR9 thallus also play a part of growth promotion role, but the growth promotion role caused by the colonization of the SQR9 at the corn rhizosphere is obviously more important, and the growth promotion roles are Y2000+ SQR9, Y1000+ SQR9 and Y5000+ SQR9 from high to low, which are consistent with the colonization result of the SQR9 at the corn rhizosphere. 14 days after seedling transplantation: compared with Blank, the plant heights treated by Y1000, Y1000+ SQR9(I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 are respectively increased by 17.3%, 20.5%, 24.1%, 32.0%, 36.4% and 26.6%, which indicates that the tobacco foam fermentation liquor has certain nutrient function, and the organic matters of the SQR9 thallus also contribute to partial growth promotion function, and in addition, the differences of Y1000, Y1000+ SQR9(I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 are obviousWherein the Y2000+ SQR9 has the best treatment effect; the fresh weight of the overground part is respectively increased by 55.6%, 60.3%, 72.2%, 115.7%, 137.8% and 99.9%, which indicates that the tobacco foam fermentation liquor has certain nutrient function, and the organic matters of the SQR9 thallus also contribute to partial growth promotion function, in addition, the differences of Y1000, Y1000+ SQR9(I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 are obvious, wherein the Y2000+ SQR9 effect is optimal; the dry weight of the overground part is respectively increased by 37.7%, 39.1%, 43.5%, 85.5%, 92.0% and 62.3%, which shows that the tobacco foam fermentation liquor has certain nutrient function, the organic matter of the SQR9 thallus also contributes to partial growth promotion function, in addition, the differences of the Water + SQR9, the Y1000+ SQR9, the Y2000+ SQR9 and the Y5000+ SQR9 are obvious, and the Y2000+ SQR9 effect is optimal. In general, nutrients of the smoke foam fermentation liquor can play a certain role in growth promotion, and organic matters of the SQR9 thallus also play a part of growth promotion role, but the growth promotion role caused by the colonization of the SQR9 at the corn rhizosphere is obviously more important, and the growth promotion roles are Y2000+ SQR9, Y1000+ SQR9 and Y5000+ SQR9 from high to low, which are consistent with the colonization result of the SQR9 at the corn rhizosphere. 28 days after seedling transplantation: compared with Blank, the plant heights of Y1000, Y1000+ SQR9(I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 are respectively increased by 11.6%, 12.9%, 17.3%, 25.7%, 20.9% and 17.8%, which indicates that the tobacco foam fermentation liquor has certain nutrient function, and the organic matters of the SQR9 thallus also contribute to partial growth promotion function, in addition, Y1000+ SQR9(I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 have obvious difference, wherein the treatment effect of Y1000+ SQR9 is optimal; fresh weight of the overground part is respectively increased by 31.5%, 52.6%, 77.5%, 108.4%, 115.7% and 81.5%, which shows that the tobacco foam fermentation liquor has certain nutrient function, organic matters of the SQR9 thallus also contribute to partial growth promotion function, in addition, the differences of Y1000+ SQR9(I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 are obvious, and the Y2000+ SQR9 effect is optimal; the dry weight of the overground part is respectively increased by 18.7%, 49.8%, 75.6%, 105.3%, 126.7% and 84.9%, which shows that the tobacco foam fermentation liquor has certain nutrient function, the organic matter of the SQR9 thallus also contributes to partial growth promotion function, and in addition, the differences of Y1000+ SQR9(I), Water + SQR9, Y1000+ SQR9, Y2000+ SQR9 and Y5000+ SQR9 are obviously shownNotably, Y2000+ SQR9 performed best. In general, nutrients of the smoke foam fermentation liquor can play a certain role in growth promotion, and organic matters of the SQR9 thallus also play a part of growth promotion role, but the growth promotion role caused by the colonization of the SQR9 at the corn rhizosphere is obviously more important, and the growth promotion roles are Y2000+ SQR9, Y1000+ SQR9 and Y5000+ SQR9 from high to low, which are consistent with the colonization result of the SQR9 at the corn rhizosphere. In conclusion, the growth promoting result and the colonization result are completely consistent, and the optimal dilution multiple of the tobacco foam fermentation liquid is 2000 times.

TABLE 1 Effect of different treatments on corn growth

Claims

1. The application of the biological fertilizer synergist in improving the colonization number of the growth-promoting bacteria SQR9 at the plant rhizosphere is characterized in that the growth-promoting bacteria SQR9 are classified and named as Bacillus amyloliquefaciens and are preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of Ministry of Western Lu 1 of North China, Kyoho of China academy of sciences, the preservation date is 2012, 2 months and 27 days, and the preservation number is CGMCC NO. 5808;

the production method of the biological fertilizer synergist comprises the following steps: the method comprises the following steps of taking tobacco waste as a fermentation substrate, inoculating domesticated river bottom sludge, and performing anaerobic fermentation to obtain a biological fertilizer synergist, wherein the production conditions of the anaerobic fermentation are as follows: the initial pH is 5-6, the inoculation amount of the domesticated river bottom sludge is 10-15 wt% of a fermentation substrate, the final water content is 85-90%, the fermentation temperature is 37-40 ℃, and the fermentation time is 140-150 h;

the method for domesticating the river bottom sludge comprises the following steps: inoculating fresh river bottom sludge into the tobacco foam waste according to the inoculation amount of 20% by mass, wherein the final water content is 85%, the fermentation temperature is 37 ℃, the initial pH is 6.0, fermenting is carried out for 4 days, the fermentation product is used as an inoculum, the tobacco foam waste is used as a fermentation substrate, and then carrying out secondary fermentation, and the fermentation product obtained by the secondary fermentation is domesticated river bottom sludge.

2. Use according to claim 1, characterized in that the solid-liquid separation is carried out by suction filtration using a buchner funnel.

3. The use according to claim 1, wherein the produced bio-fertilizer synergist is a liquid.