CN110041138A - A kind of method and product using acidolysis amino acid and biogas residue production biological organic fertilizer - Google Patents
A kind of method and product using acidolysis amino acid and biogas residue production biological organic fertilizer Download PDFInfo
- Publication number
- CN110041138A CN110041138A CN201910447595.8A CN201910447595A CN110041138A CN 110041138 A CN110041138 A CN 110041138A CN 201910447595 A CN201910447595 A CN 201910447595A CN 110041138 A CN110041138 A CN 110041138A
- Authority
- CN
- China
- Prior art keywords
- biogas residue
- organic fertilizer
- amino acid
- biological organic
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of methods and product using acidolysis amino acid and biogas residue production biological organic fertilizer.A method of biogas residue biological organic fertilizer is made using acidolysis amino acid and biogas residue, comprising the following steps: by acidolysis amino acid with 0.2mL g‑1(dry weight) is added decomposed biogas residue organic fertilizer and is mixed, and interior is banked up 4-5 days, and daily turning is primary, when pH gos up to 5-6 by 0.05mL g‑1Ratio inoculum density be 1 × 109CFU mL‑1Bacillus amyloliquefaciens SQR9 fermentation liquid, daily turning is primary, fermentation 3-4 days after, coating monitor growth-promoting bacterial content reach 1 × 108CFU g‑1Terminate to ferment above, the water content of tunning is finally evaporated to 30% hereinafter, as finished product biogas residue biological organic fertilizer under conditions of temperature is no more than 50 DEG C.Had using the invention biological organic fertilizer and be rich in functional microorganism and free amino acid, the good advantage of growth-promoting effect is suitble to the industrial crops such as fruits and vegetables and fruit tree plantation large area to use.The technical matters, which is that biogas residue is high-valued, provides technical support.
Description
Technical field
The invention belongs to microbial manure field, it is related to a kind of producing biology using acidolysis amino acid and biogas residue organic fertilizer and having
The method and product of machine fertilizer.
Background technique
At this stage, due to a large amount of intensivization development using especially industrialized agriculture of chemical fertilizer in the late four decades, lead to me
State's soil regime sharply declines.The problems such as continuous cropping obstacle, secondary salinization, soil hardening, causes the high yield of agricultural product to be difficult to tie up
It holds, quality degradation.Organic fertilizer as improvement soil texture, improve biological activity of soil, promoted soil productivity it is good simply
Medicine, since the problems such as short-term response is slow, this season crop yield is unobvious is not come into operation largely always by our agricultural producers.
Therefore, it is necessary to research and develop that fertilizing soil but also the bio-organic fertilizer of crop yield, quality can be improved.Biological organic fertilizer refers to
Microorganisms with specific functions with mainly with plant and animal residues (such as crop stalk, feces of livestock and poultry) for source, and through innoxious place
One kind that reason, decomposed organic materials are combined has both the fertilizer of microbial manure and organic fertilizer effect.Its substantive characteristics is
Microorganism containing specific function, showing certain fertilizer effect, the vital movement of these functional microorganisms are that biology has
Machine fertilizer is better than the key factor of other fertilizer.After biological organic fertilizer application can phosphorus in fixed nitrogen and activating soil and potassium to improve
Soil fertility is simultaneously conducive to crops absorption nutrient, generates antibacterial substance and growth hormone to reduce the hair of crop soil-borne disease insect pest
It gives birth to and promotes crop robust growth.
With increasingly sharpening for oil crisis and problem of environmental pollution, biogas fermentation technology is also increasingly valued by the people.
While biogas fermentation technology offers convenience to people's lives, a large amount of anaerobic fermentation residues will necessarily be also generated ---
Biogas residue.In biogas residue the content of organic matter be 36-49.9%, content of humic acid 10.1-24.6%, total nitrogen content 0.78-1.61%, entirely
Phosphorus content 0.39-0.71%, full potassium content 0.61-1.3%.But since biogas residue is formed under anaerobic, there is nutrient
Content is low, carbon-nitrogen ratio is unreasonable, functional microorganism flora amount and activity are lower, causes to make fertile period length and biogas residue product is low
The problems such as value.In general production, most of biogas residue is applied directly in soil, while causing pollution of area source
It is a kind of waste of organic solid resource.Therefore, the present invention is quasi- by adding amino acid to supplement the available nutrient in biogas residue, so
Addition function bacterium carries out secondary fermentation and is developed into biological organic fertilizer afterwards.It is organic to solve biogas residue while by biogas residue higher value application
Matter and nutrient content are relatively low, make the problem that common organic fertilizer is relatively inaccessible to professional standard.
Currently, China's intensive aquaculture of height improves animal natural death rate, if animals died of illness cannot be timely
Harmless treatment is thoroughly carried out, the generation and propagation of animal epidemic is will lead to, brings great threat to aquaculture.Based on state
Successfully by sick dead pig, through sulfuric acid, acidolysis completely solves pathogen at amino-acid liquid at high temperature for interior current research
Harm, and free amino acid and polypeptide (number of patent application: 201410042218.3) are converted by sick dead pig albumen.This patent will
Biological organic fertilizer is researched and developed in conjunction with biogas residue using this acid hydrolysis solution, solves biogas residue higher value application and sick dead pig handles difficult ask
Topic.
After invention will be mixed using cheap acid hydrolysis solution with biogas residue, access function bacterial strain carries out secondary solid
Fermentation generates biogas residue biological organic fertilizer.The difficult problem of biogas residue fertilizer commercialization is not only solved, and reduces biological organic fertilizer
Production cost, extend the production raw material of biological organic fertilizer.
Summary of the invention:
Present invention aims at the problem for being directed to biogas residue fertilizer higher value application hardly possible, developing a kind of using biogas residue is biological
Raw material in organic fertilizer rich in a large amount of functional microorganisms and is dissociated by secondary fermentation function bacterium production after addition acidolysis amino acid
The formula and technique of the biological organic fertilizer of amino acid.
A method of biogas residue biological organic fertilizer being made using acidolysis amino acid and biogas residue, method includes the following steps:
By acidolysis amino acid with 0.05-0.3mL g-1(dry weight) is added decomposed biogas residue organic fertilizer and is mixed, and interior is banked up
4-5 days, daily turning was primary, detected the dynamic change of pH, when the pH of decomposed biogas residue organic fertilizer go up to 5-6 be added gemma amount it is big
In 1 × 109CFU mL-1Function bacterium SQR9 (CGMCC NO.5808) fermentation liquid, daily turning is primary, fermentation 3-4 days after, painting
Cloth monitoring growth-promoting bacterial content reaches 1 × 108CFU g-1Terminate to ferment above, it finally will hair under conditions of temperature is no more than 50 DEG C
The water content of ferment product is evaporated to 30% hereinafter, as finished product biogas residue biological organic fertilizer.
Preferably as one kind of the invention, by acidolysis amino acid with 0.14-0.23mL g-1Decomposed biogas residue, which is added, in (dry weight) has
Machine fertilizer.
As present invention further optimization, by acidolysis amino acid with 0.2mL g-1It is organic that decomposed biogas residue is added in (dry weight)
Fertilizer.
Preferably as one kind of the invention, the acidolysis amino acid is using sulfuric acid at 100-105 DEG C to discarded poultry
Fowl hydrolysis is made, or directly buys commercially available acidolysis amino acid products, and in one embodiment, the acidolysis amino acid is purchased from river
Yin Pengyaolian industry Biotechnology Co., Ltd, the hydrogen ion concentration of acid hydrolysis solution are 3mol, and total amino acid content is 10%.
Decomposed biogas residue organic fertilizer of the present invention can be the decomposed biogas residue prepared according to conventional technical means in the art,
Commercially available decomposed biogas residue organic fertilizer can also be bought；In one embodiment of the invention, the decomposed biogas residue organic fertilizer is purchased from
Venus (sunshine) agricultural science and technology Co., Ltd.
Preferably as one kind of the invention, the function bacterium SQR9 fermentation liquid is by function bacterium SQR9 in LB Liquid Culture
30-37 DEG C of mixing speed is 170-200rpm in base, under pH natural conditions culture bacterium or Number of spores into fermentation liquid be greater than 1 ×
109CFU mL-1, then with 6000rmin-1It is centrifuged 5min, it is spare that thallus finally is resuspended with equivalent sterile water.
The biogas residue biological organic fertilizer prepared according to the method described in the present invention.
Function bacterium viable count is greater than 1 × 10 in the biogas residue biological organic fertilizer8CFU g-1, total nitrogen content 1.5-
2.5%, total nitrogen
Phosphorus potassium nutrition is 5-6%, and the content of organic matter is greater than 40%, and germination index is greater than 95%.
Biogas residue biological organic fertilizer of the present invention is promoting the application in fruits and vegetables growth；It is preferred that in promoting vegetable growth
Application；Further preferably applied in promoting cucumber and/or tomato growth.
Should meet according to the product researched and developed of the present invention can promote fruits and vegetables plant strain growth, and function bacterium viable count is greater than 1 ×
108CFUg-1, total nitrogen content 1.5-2.5%, total npk nutrient is 5-6%, and the content of organic matter is greater than 40%, germination index
Greater than 95%.
The utility model has the advantages that
Had using the biological organic fertilizer that the invention produces rich in functional microorganism and free amino acid, growth-promoting effect is good
Advantage is suitble to the industrial crops such as fruits and vegetables and fruit tree plantation large area to use.Application of the invention, for biogas residue system fertilizer and high level
Change, preserve the ecological environment, improves quality of agricultural product and be all of great significance.
There are following advantages compared with the product that circulates currently on the market by the present invention:
1) present invention reduces the raw materials for production cost of product, improves the biological organic fertilizer market competitiveness.
2) present invention uses biogas residue, makes full use of the organic resource of biogas residue, realization is turned waste into wealth.
3) amino acid resource abundant can promote plant growth in the present invention, and test result shows to make in vegetable sprout term
After invention fertilizer, plant growth can be remarkably promoted.
4) acidolysis aminoacids solution has abundant and A wide selection of colours and designs amino acid, can effectively promote function bacterium growth, develop
High efficient strain content is greater than 1 × 10 in successful biological organic fertilizer8CFU g-1More than, advantage group can be formed after being manured into soil,
It improves function bacterium field planting in the soil and plays the ability of growth-promoting functions.
Detailed description of the invention:
Function bacterium quantity changes after Fig. 1 adds the decomposed biogas residue organic fertilizer access Promoting bacteria SQR9 fermentation of different proportion acid hydrolysis solution
Fig. 2 adds the decomposed biogas residue compost pH situation of change of different proportion acid hydrolysis solution
Function bacterium quantity changes after Fig. 3 adds the decomposed biogas residue organic fertilizer access Promoting bacteria SQR9 fermentation of different proportion acid hydrolysis solution
Fig. 4 adds the decomposed biogas residue compost pH situation of change of different proportion acid hydrolysis solution
The produced biological organic fertilizer cucumber of the decomposed biogas residue organic fertilizer addition acid hydrolysis solution of Fig. 5 tests growth-promoting figure
The produced biological organic fertilizer tomato of the decomposed biogas residue organic fertilizer addition acid hydrolysis solution of Fig. 6 tests growth-promoting figure
Specific embodiment:
Embodiment 1
1. the acquisition of acid hydrolysis solution, biogas residue
Acid hydrolysis solution of the present invention is to hydrolyze to be made to discarded livestock and poultry using sulfuric acid at 100-105 DEG C (to be purchased from Jiangyin
Peng Yaolian industry Biotechnology Co., Ltd), the hydrogen ion concentration of acid hydrolysis solution is 3mol L-1, total free amino acid content is
10%.
Heretofore described biogas residue is raw material；The decomposed biogas residue is purchased from the limited public affairs of Venus (sunshine) agricultural science and technology
Department.In the biogas residue, content of organic matter 38-42%, nitrogenous, phosphorus pentoxide, potassium oxide total amount are between 4-5%.
2. the preparation of function microbial inoculum
Function bacterium SQR9 is inoculated into LB culture solution, production, the working condition of the strain are carried out liquid fermentation are as follows: pH is raw
For long range naturally, cultivation temperature scope control is at 30-37 DEG C, mixing speed 180-200rpm, the fermentation middle and later periods forms bud
Spore makes to be more than or equal to 1 × 10 containing bacterium or Number of spores in fermentation liquid8CFU mL-1, by taking the configuration of the LB culture solution of 1L as an example, match
Set method are as follows: weigh 10g tryptone, 5g yeast extract and 10g NaCl, 900mL deionized water dissolving is added, adjust pH
To 7.0, it finally is settled to 1000mL with deionized water, 121 DEG C of high pressure steam sterilization 20min (are added 2% in prescription of culture liquid
LB solid medium is made in the agar of m/v).SQR9 bacterium is seeded to LB liquid medium after the activation of LB solid medium,
In 170rmin-1, under the conditions of 30 DEG C liquid fermentation and culture for 24 hours, then with 6000rmin-1It is centrifuged 5min, it is finally sterile with equivalent
It is spare that thallus is resuspended in water.
3. solid fermentation prepares biological organic fertilizer
3.1 solid fermentation formulation optimizations
The first step, primary dcreening operation are suitble to the acidolysis amino acid of function bacterium SQR9 growth to add concentration: different proportion amino acid is molten
(0.05,0.1,0.2,0.3mLg in the biogas residue after packing is added in liquid-15%, 10%, 20%, 30% is respectively represented, ammonia is not added
Base acid is CK processing), control moisture carries out composting, measures pH and water-cut variation daily.After pH rises to 5-6, each place
5% (v/w) concentration that reason prepares before being added is 1 × 109CFU mL-1SQR9 bacterium solution (final concentration of the 5 of strain after addition ×
107CFU g-1), carry out secondary fermentation.Daily statistical function bacterium SQR9 quantity, fermentation period are 6 days.The function bacterium quantity of monitoring
Variation is as shown in Figure 1, the general trend of function bacterium growth is first to rise to decline afterwards.When amino acid concentration is 20%, function bacterium
SQR9 quantity is 1.9 × 108CFU g-1, be significantly higher than 5%, 10%, 30% and do not add amino acid CK processing in function bacterium
Quantity.Water-cut variation controls between 30-60%.PH variation is as shown in Fig. 2, all processing are in rising trend, and most
Reach 5 or more eventually, meets the growth requirement of function bacterium SQR9.Therefore, deducibility is in this invention when amino acid concentration is
When 20%, the quantity of function bacterium SQR9 is highest.The amino acid additive amount for primarily determining the most suitable growth of function bacterium SQR9 is
20% or so.
The most suitable acidolysis amino acid of second step, further screening function bacterium SQR9 growth adds concentration: by different proportion ammonia
(0.14,0.17,0.20,0.23mLg in the biogas residue after packing is added in base acid solution-1Respectively represent 14%, 17%, 20%,
23%, do not add amino acid as CK processing), control moisture carries out composting, measures pH and water-cut variation daily.It is risen to pH
After 5-6, it is 1 × 10 that 5% (v/w) concentration, which is added, in each processing9CFU mL-1SQR9 bacterium solution (final concentration of the 5 of strain after addition ×
107CFU g-1), carry out secondary fermentation.Fermentation period is 6 days, daily statistical function bacterium number amount.Data analysis through experimental result
Know that the quantity of function bacterium SQR9 can be greater than 1 × 108CFU g-1.Fig. 3 shows under identical experiment environmental condition, with compost day
Several increase function bacterium SQR9 quantity general trends shows downward trend after first rising, the function of different aminoacids content processing
Energy bacterium SQR9 volume peaks appear in the third day of fermentation.When amino acid concentration be 20% when, function bacterium SQR9 quantity be 1.4 ×
108CFU mL-1It is significantly higher than 14%, 17%, 23% and does not add the function bacterium quantity of the CK processing of amino acid.Moisture content becomes
Change, entirety is in downward trend after first rising and moisture content controls between 40-60% always.PH changes as shown in figure 4, pH is whole
It is in rising trend, 5 or more are finally all risen to, can achieve the pH requirement for the growth for meeting function bacterium SQR9.It is verified, the bottom of at
Object is in the growing environment of biogas residue, and function bacterium SQR9 can be with smooth growth successfully to prepare novel biological fertilizer.Determination is most suitable
The concentration that conjunction prepares amino acid processing is 20%.
In conclusion acid hydrolysis solution in decomposed biogas residue can pH bank up rise naturally to 5 or more, and be suitble to function bacterium SQR9
Fermentation, when acid hydrolysis solution additive amount be 0.2mLg-1When for function bacterium growth optimum ratio.
The fermentation of 3.2 solid rafts generates finished product organic fertilizer
According to above-mentioned formula and technique, by acid hydrolysis solution with 0.2mLg-lIt is respectively connected to biogas residue after evenly mixing, daily turning one
Secondary, composting 2-5 days, when pH is 5-6,5% (v/w) concentration of access was 1 × 109CFU mL-1Growth-promoting function bacterium SQR9 hair
Zymotic fluid continues secondary fermentation 3-4 days.Daily turning 1 time, control solid fermentation temperature is no more than 50 DEG C.Product after fermentation
Middle function bacterium quantity is greater than 1 × l08CFU g-1, it is higher than biological organic fertilizer professional standard.
The experiment of 2 greenhouse pot culture of embodiment
1. biological organic fertilizer is in the not equal nutrients pot experiment of cucumber
Equipped with crop cucumber, which sets a control and six processing.Control treatment is CK: vermiculite cultivation, which is not added, appoints
What fertilizer, six processing are respectively OF: common biogas residue organic fertilizer made of decomposed biogas residue；BIO0: decomposed biogas residue directly adds rush
Biological organic fertilizer made of raw bacterium SQR9；BIO14: decomposed biogas residue adds 0.14mL g-1Acid hydrolysis solution and pH are inoculated with growth-promoting after ging up
Biological organic fertilizer made of bacterium SQR9 secondary fermentation；BIO17: decomposed biogas residue adds 0.17mL g-1Acid hydrolysis solution and pH rise be followed by
Biological organic fertilizer made of kind Promoting bacteria SQR9 secondary fermentation；BIO20: decomposed biogas residue adds 0.20mL g-1Acid hydrolysis solution and pH are returned
Biological organic fertilizer made of Promoting bacteria SQR9 secondary fermentation is inoculated with after rising；BIO23: decomposed biogas residue adds 0.23mL g-1Acid hydrolysis solution
And biological organic fertilizer made of Promoting bacteria SQR9 secondary fermentation is inoculated with after pH rise.14 basins of each processing, every basin fill vermiculite 50g,
Fertilizer additive amount is 2.5g, and as a result such as Fig. 5, details are shown in Table 1.
Table 1: influence of the different disposal to cucumber character
Known to as shown in Table 1: in the Biological control of cucumber, OF, BIOO, BIO14, BIO17, BIO20, BIO23 are everywhere
Reason is all remarkably higher than CK processing in plant height, stem thickness, leaf width, overground part fresh weight and underground part fresh weight, this is because CK is in without feeding
Isloation state.Compare CK, OF processing with BIOO, BI014, BI017, BI020, BI023 respectively handle it can be found that add amino acid and
Product after function bacterium fermentation remarkably promotes cucumber growth.In addition to the data of stem thickness processing, the plant height of BIO20, BI023, leaf width,
Overground part fresh weight and underground part fresh weight numerical value are above CK, OF, BIOO, BI014, BI017 processing numerical value.Due to BIO20,
Plant height, stem thickness, leaf width, overground part fresh weight and the underground part fresh weight no significant difference of BI023, but the amino acid of BIO20 processing adds
Dosage ratio BIO23 is few, and cost is lower, therefore handles better than BI023.
2. not equal nutrients pot experiment of the biological organic fertilizer on tomato
Equipped with crop tomato, which sets six processing of a control.Control treatment is CK: vermiculite cultivation is not added any
Fertilizer, six processing are respectively OF: common biogas residue organic fertilizer made of decomposed biogas residue；BIO0: decomposed biogas residue directly adds growth-promoting
Biological organic fertilizer made of bacterium SQR9；BIO14: decomposed biogas residue adds 0.14mL g-1Acid hydrolysis solution and pH are inoculated with Promoting bacteria after ging up
Biological organic fertilizer made of SQR9 secondary fermentation；BIO17: decomposed biogas residue adds 0.17mL g-1Acid hydrolysis solution and pH are inoculated with after ging up
Biological organic fertilizer made of Promoting bacteria SQR9 secondary fermentation；BIO20: decomposed biogas residue adds 0.20mL g-1Acid hydrolysis solution and pH rise
It is inoculated with biological organic fertilizer made of Promoting bacteria SQR9 secondary fermentation afterwards；BIO23: decomposed biogas residue adds 0.23mL g-1Acid hydrolysis solution and
PH is inoculated with biological organic fertilizer made of Promoting bacteria SQR9 secondary fermentation after ging up.14 basins of each processing, every basin fill vermiculite 50g, fertilizer
Material additive amount is 2.5g, and as a result such as Fig. 6, details are shown in Table 2.
Table 2: influence of the different disposal to tomato character
As shown in Table 2, in tomato Biological control, OF, BIOO, BIO14, BIO17, BIO20, BIO23 respectively handle plant height,
Stem thickness, leaf width, overground part fresh weight and underground part fresh weight are all remarkably higher than CK processing, this is because CK is in no nutrient status.It removes
Outside overground part fresh weight data, compares CK, OF processing and respectively handle with BIOO, BI014, BI017, BI020, BI023 it can be found that adding
Product after adding amino acid and function bacterium to ferment remarkably promotes tomato growth.Due to the plant height of BIO20, stem thickness, overground part fresh weight
It is above CK, OF, BIO0, BIO14, BIO17, BIO23 processing numerical value with underground part fresh weight numerical value, this shows BIO20 processing pair
The growth of tomato has apparent growth-promoting functions, it is thus determined that optimal processing is BI020.
Solid fermentation shows that this research formula can remarkably promote function bacteria solid fermentation, increases Promoting bacteria in fertilizer
Quantity and fertilizer nutrient, acid hydrolysis solution input amount 0.2mL g-1For function bacterium optimum concentration, can significantly reduce biological organic fertilizer at
This, increases added value of product.Meanwhile discarded biogas residue utilization rate can be improved in the formula, also provides branch to solve animals died of illness
Support mitigates environmental pollution.Therefore, the biological organic fertilizer formula of this patent research and development and technique have very strong specific aim and practical
Property.
Claims (10)
1. a kind of method using acidolysis amino acid and biogas residue production biogas residue biological organic fertilizer, which is characterized in that this method includes
Following steps:
By acidolysis amino acid with 0.05-0.3mL g-1(dry weight) is added decomposed biogas residue organic fertilizer and is mixed, and 4-5 is banked up in interior
It, daily turning is primary, detects the dynamic change of pH, and gemma amount is added greater than 1 to 5-6 when the pH of decomposed biogas residue organic fertilizer gos up
×109CFU mL-1Function bacterium SQR9 fermentation liquid, daily turning is primary, fermentation 3-4 days after, coating monitor growth-promoting bacterial content reach
To 1 × 108CFU g-1Terminate to ferment above, finally evaporates the water content of tunning under conditions of temperature is no more than 50 DEG C
To 30% hereinafter, as finished product biogas residue biological organic fertilizer.
2. the method according to claim 1, wherein by acidolysis amino acid with 0.14-0.23mL g-1(dry weight) adds
Enter decomposed biogas residue organic fertilizer.
3. the method according to claim 1, wherein by acidolysis amino acid with 0.2mL g-1(dry weight) is added decomposed
Biogas residue organic fertilizer.
4. the method according to claim 1, wherein the acidolysis amino acid is to utilize at 100-105 DEG C
Sulfuric acid hydrolyzes discarded livestock and poultry and is made, or directly buys commercially available acidolysis amino acid products.
5. the method according to claim 1, wherein the function bacterium SQR9 fermentation liquid is by function bacterium SQR9
In LB culture medium 30-37 DEG C of mixing speed be 170-200rpm cultivate into fermentation liquid bacterium or Number of spores be greater than 1 ×
109CFU mL-1。
6. the biogas residue biological organic fertilizer prepared according to method of any of claims 1-5.
7. biogas residue biological organic fertilizer according to claim 6, which is characterized in that function in the biogas residue bio-organic fertilizer product
Bacterium viable count is greater than 1 × 108CFU g-1, total nitrogen content 1.5-2.5%, total npk nutrient is 5-6%, and the content of organic matter is big
In 40%, germination index is greater than 95%.
8. biogas residue biological organic fertilizer as claimed in claim 6 is promoting the application in fruits and vegetables growth.
9. application according to claim 8, which is characterized in that the biogas residue biological organic fertilizer is in promoting vegetable growth
Application.
10. application according to claim 9, which is characterized in that the biogas residue biological organic fertilizer promote cucumber and/or
It is applied in tomato growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910447595.8A CN110041138A (en) | 2019-05-27 | 2019-05-27 | A kind of method and product using acidolysis amino acid and biogas residue production biological organic fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910447595.8A CN110041138A (en) | 2019-05-27 | 2019-05-27 | A kind of method and product using acidolysis amino acid and biogas residue production biological organic fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110041138A true CN110041138A (en) | 2019-07-23 |
Family
ID=67283755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910447595.8A Pending CN110041138A (en) | 2019-05-27 | 2019-05-27 | A kind of method and product using acidolysis amino acid and biogas residue production biological organic fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110041138A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112830827A (en) * | 2021-01-27 | 2021-05-25 | 南京农业大学 | Bio-organic fertilizer prepared from pecan peel compost as well as preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104788143A (en) * | 2015-03-31 | 2015-07-22 | 南京农业大学 | Method for producing bio-organic fertilizer from acidolysis solution and decomposed manure and product |
| CN105060952A (en) * | 2015-07-19 | 2015-11-18 | 成都市朝森有机肥有限公司 | Amino acid microbial organic fertilizer |
| CN107805091A (en) * | 2017-11-29 | 2018-03-16 | 江苏苏港和顺生物科技有限公司 | It is a kind of to prepare the production system of organic fertilizer and organic fertilizer prepared therefrom using biogas residue |
-
2019
- 2019-05-27 CN CN201910447595.8A patent/CN110041138A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104788143A (en) * | 2015-03-31 | 2015-07-22 | 南京农业大学 | Method for producing bio-organic fertilizer from acidolysis solution and decomposed manure and product |
| CN105060952A (en) * | 2015-07-19 | 2015-11-18 | 成都市朝森有机肥有限公司 | Amino acid microbial organic fertilizer |
| CN107805091A (en) * | 2017-11-29 | 2018-03-16 | 江苏苏港和顺生物科技有限公司 | It is a kind of to prepare the production system of organic fertilizer and organic fertilizer prepared therefrom using biogas residue |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112830827A (en) * | 2021-01-27 | 2021-05-25 | 南京农业大学 | Bio-organic fertilizer prepared from pecan peel compost as well as preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN103980017B (en) | The method of the quick production high quality organic fertilizer of high-temperature aerobic Secondary Fermentation | |
| CN101870608B (en) | Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof | |
| CN104788143B (en) | A kind of utilization acid hydrolysis solution produces the method and product of biological organic fertilizer with decomposed manure | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN102399096A (en) | Method of producing organic fertilizer by using Chinese herb residue | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN103553810A (en) | Preparation method of composite biological organic fertilizer granules | |
| CN106754463B (en) | One plant of tool dissolving P capacity Burkholderia bacterium NJAU-B8 and its microbial manure of development | |
| CN102432355A (en) | Banana stem organic fertilizer and preparation method thereof | |
| CN109265245A (en) | A kind of stalk fertilizer bio-fermented liquid and preparation method thereof | |
| CN109400384A (en) | Biological organic fertilizer for preventing and treating rice blast and application method thereof | |
| CN106045677A (en) | Method for preparing organic compound biological fertilizer special for fruit trees | |
| CN106699474A (en) | Novel bio-organic fertilizer and preparation method thereof | |
| CN1924004A (en) | Organism preparation method of agricultural amino acid and fertilizer product | |
| CN107903092A (en) | A kind of method and application using mushroom bran discarded object production bio-feritlizer synergist | |
| CN105481486B (en) | Utilize the product of the method and acquisition of stalk and filter mud production trichoderma as biological organic fertilizer | |
| CN101851123A (en) | High-concentration compound microorganism bacterial fertilizer and production method thereof | |
| CN103173387B (en) | Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer | |
| CN102219575B (en) | Industrial method for producing bio-fertilizer inoculant by utilizing decayed cow dung as raw material | |
| CN103409351A (en) | Growth promoting strain used for promoting banana growth and microbial organic fertilizer produced with same | |
| CN102924136A (en) | Process for utilizing abandoned feathers to produce special bio-organic fertilizer for bananas and product thereof | |
| CN108383653A (en) | A kind of method and bio-organic fertilizer preparing fertilizer using stalk | |
| CN110041138A (en) | A kind of method and product using acidolysis amino acid and biogas residue production biological organic fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190723 |
|
| RJ01 | Rejection of invention patent application after publication |