CN102191179A - Method for culturing marine oil-producing microalgae - Google Patents
Method for culturing marine oil-producing microalgae Download PDFInfo
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- CN102191179A CN102191179A CN 201110104351 CN201110104351A CN102191179A CN 102191179 A CN102191179 A CN 102191179A CN 201110104351 CN201110104351 CN 201110104351 CN 201110104351 A CN201110104351 A CN 201110104351A CN 102191179 A CN102191179 A CN 102191179A
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Abstract
The invention discloses a method for culturing marine oil-producing microalgae, which is characterized in that: the marine microalgae are culture by using daily sewage to supply nutritional salts and using high-concentration carbon dioxide to provide a carbon source, so the biomass yield of the marine microalgae is improved; and the microalgae are subjected to direct high-light exposure so as to improve the oil and fat yield of the marine microalgae. In the invention, the culture cost of the oil-enriched marine microalgae is lowered, the process of inducing the oil and fat accumulation in microalgae is simplified, and the industrialization of the large-scale culture of oil-enriched marine microalgae is promoted.
Description
Technical field
The present invention relates to little algae culture technique, relate in particular to the high-density cultivation method of the little algae of a kind of ocean produce oil, can be widely used in the production of rich oil marine microalgae biomass.
Background technology
Little algae is the cell factory that a sunlight drives, by the CO of photosynthesis absorption efficiently
2, luminous energy is converted into the chemical energy of compounds such as fat or starch, and emits O
2Utilize little algal biomass production biofuel, can effectively solve the raw material bottleneck of biofuel industry, for the health of biological energy industry, stable, sustainable development provide new road for development.Little algae is photoautotrophic hydrobiont, and both culturing microalgae needs a large amount of water, if cultivate little algae with the runway pond, annual per hectare (ha) needs 1.1～1.3 * 10
7The water that rises.Tellurian ocean area accounts for 71% of ground sphere area, and seawater is the resource of a kind of " inexhaustible, nexhaustible ".Therefore, marine microalgae since can be in seawater growth and breeding, have not the advantage of striving ground, not striving water with the people with grain, in the development of little algae biofuel industry, have very consequence.Problems such as yet nutritive element contents such as the nitrogen phosphorus in the seawater are lower, when therefore utilizing seawater to cultivate marine microalgae separately, have poor growth, and biomass is low will increase little algae cultivation cost yet manually add nutritive salt.In addition, the carbon in the seawater can not satisfy little algae high growth rates and the required carbon level of biomass yield far away, in little algae large scale culturing process, if can utilize the high concentration CO of industrial discharge
2For little algae provides carbon source, can not only provide enough carbon sources for micro algae growth, and can reduce the discharging of greenhouse gases.
So far, the common problem that the marine microalgae culture density is low, cost is high does not obtain fine solution; In addition, present common employing " two-step approach " nitrogen stress induces little algae to accumulate a large amount of greases, and this method need accumulate certain biomass after centrifugal collection in little algae, little algae is suspended in the nitrogen stress substratum again induces produce oil, increase extra energy input, improved the production cost of oil-rich microalgae.
Summary of the invention
The object of the present invention is to provide the cultural method of the little algae of a kind of ocean produce oil.To overcome the defective that exists in the known technology.
For achieving the above object, the cultural method of the little algae of ocean produce oil provided by the invention, be to provide nutritive salt, high concentration carbon dioxide that carbon source is provided with sanitary sewage, cultivate the yield of biomass of marine microalgae, and expose raising marine microalgae fat content by the direct high light of little algae with the raising marine microalgae.
In the cultural method of the little algae of described ocean produce oil, the volume ratio that sanitary sewage accounts for seawater is 10～100%.
In the cultural method of the little algae of described ocean produce oil, CO in the high concentration carbon dioxide gas
2Content be 5%～100%.
In the cultural method of the little algae of described ocean produce oil, little algae culture condition is: culture temperature is 23～27 ℃, continuous illumination and ventilation, and intensity of illumination is 60～100 μ mol m
-2s
-1, Ventilation Rate is 0.1～0.3L/min.
In the cultural method of the little algae of described ocean produce oil, high gloss oil fat inductive light intensity is: 150～300 μ mol m
-2s
-1
In the cultural method of the little algae of described ocean produce oil, as the sanitary sewage that nutritive salt is provided is that the sanitary sewage through second-stage treatment is carried out pre-treatment again, after filtering out the large granular impurity in the sewage, add 10～100 μ l/L chlorine bleach liquores and disinfected 12～48 hours.
In the cultural method of the little algae of described ocean produce oil, used marine microalgae is little plan ball algae.
Compared with prior art, the present invention has following advantage:
1) utilizes sanitary sewage to replace nutritive salt in the sea water medium, reduced the cultivation cost of marine microalgae, improved the biomass of marine microalgae.Add the biomass of marine microalgae behind the sanitary sewage and be conventional substratum 1.1～1.6 times;
2) the high concentration carbon dioxide gas of Bu Chonging provides carbon source for marine microalgae, has promoted the accumulation of marine microalgae biomass, and the biomass of its accumulation is 1.9～3.2 times that replenish under the air conditions, for little algae absorbs CO in the industrial flue gas
2Accumulates biomass is laid a good foundation, and energy-saving and emission-reduction, protection environment are had great importance;
3) by being directly exposed to, marine microalgae obviously improved marine microalgae grease productive rate under the high light intensity condition, simplified the process of inducing the microalgae grease accumulation, be convenient to implement, and cost is low, be suitable for scale operation rich oil marine microalgae biomass, inducing for marine microalgae is greasy provides, a high-efficiency method simpler, energy-conservation than " two-step approach ".Fat content is significantly improved after the high photoinduction, and fat content is 1.1～2.1 times before inducing.
Description of drawings
Fig. 1 is the growth curve (is example with little plan ball algae) of marine microalgae in the different ratios sanitary sewage.
The growth curve (is example with little plan ball algae) of marine microalgae when Fig. 2 is culture medium supplemented air and high concentration carbon dioxide cultivation for the same ratio sanitary sewage.
Fig. 3 changes (is example with little plan ball algae) for fat content in the little gonidium in ocean under high photoinduction and the conventional culture condition under the additional high concentration carbon dioxide condition.
Embodiment
The present invention utilizes sanitary sewage to substitute nutritive salt, replenishes high concentration CO
2Provide carbon source to accelerate the growth of marine microalgae, and improve fat content in little gonidium by directly being exposed under the high light intensity condition, reduce the cultivation cost of rich oil marine microalgae, simplified the process of inducing the microalgae grease accumulation, helped the industrialization of rich oil marine microalgae large scale culturing.
Technical scheme of the present invention is as follows:
1) optimization of sanitary sewage ratio: after adding natural sea-water to by 10～100% volume ratio, inoculate, under illumination condition, leave standstill cultivation with fresh marine microalgae algae kind through second-stage treatment and filtering sanitary sewage;
2) utilize sanitary sewage and high concentration carbon dioxide gas to carry out the cultivation of marine microalgae: adding in the bioreactor after the sanitary sewage of aforementioned proportion and the sea water mixing, and to add marine microalgae algae kind, feed high concentration carbon dioxide (CO wherein
2Concentration is 5～100%) cultivate, cultivate and grew into the logarithmic growth middle and later periods until microalgae cell in 6～8 days.Culture temperature is that 23～27 ℃, intensity of illumination are 60～100 μ mol m
-2s
-1, Ventilation Rate is 0.1～0.3L/min, 24h continuous illumination and ventilation;
3) high photoinduction oil and fat accumulation: under additional carbon dioxide gas condition, above-mentioned little algae algae liquid is exposed to 150～300 μ mol m
-2s
-1Under the light intensity condition, induce the oil and fat accumulation in the microalgae cell.
Below in conjunction with embodiment the present invention is done detailed explanation.
Step 1: the optimization of sanitary sewage ratio.Based on natural sea-water, add volume respectively and be 10%, 30%, 50%, 80% and 100% behind second-stage treatment and filtering sanitary sewage, inoculate with fresh little plan ball algae, and with conventional f/2 substratum in contrast.In 25 ℃ of temperature, intensity of illumination is 70 μ molm
-2s
-1Condition under leave standstill and cultivated 6 days.With reference to Fig. 1, the result shows through test of many times, and more suitable sanitary sewage ratio is 30%～50%, and the biomass that little plan ball algae accumulates in this scope is 1.2～1.6 times of control medium.
Step 2: utilize sanitary sewage and high concentration carbon dioxide to carry out the cultivation of little plan ball algae.The sanitary sewage of interpolation 50% adds fresh little plan ball algae algae kind as substratum in natural sea-water, feeds 15%CO behind the 24h
2Cultivate, grow into the logarithmic growth middle and later periods until little plan ball frustule, control group fills air and cultivates.Culture temperature is that 25 ℃, intensity of illumination are 70 μ mol m
-2s
-1, Ventilation Rate is 0.1L/min, 24h continuous illumination and ventilation.As Fig. 2, the result shows through test of many times, cultivates generally that little plan ball frustule grows into the logarithmic growth middle and later periods after 4～8 days, and the biomass of little plan ball algae accumulation is 2.1～3.2 times when filling air and cultivating.
Step 3: high photoinduction oil and fat accumulation.The above-mentioned little plan ball algae algae liquid that enters the logarithmic growth middle and later periods is exposed to 150 μ mol m
-2s
-1Under the light intensity condition, and continue to feed 15%CO with the flow velocity of 0.1L/min
2, induce the oil and fat accumulation in the microalgae cell, it is constant that control group is cultivated light intensity, and promptly low light is cultivated.As Fig. 3, show that through experimental result repeatedly the fat content of little plan ball algae under high optical condition is 1.1～1.9 times before inducing.
Claims (7)
1. the cultural method of the little algae of ocean produce oil, it is characterized in that, provide nutritive salt, carbonic acid gas that carbon source is provided with sanitary sewage, cultivate the yield of biomass of marine microalgae, and expose raising marine microalgae fat content by the direct high light of little algae with the raising marine microalgae.
2. the cultural method of the little algae of claim 1 described ocean produce oil is characterized in that, the volume ratio that the sanitary sewage addition accounts for seawater is 10～100%.
3. the cultural method of the little algae of claim 1 described ocean produce oil is characterized in that, CO in the carbon dioxide gas
2Content be 5%～100%.
4. the cultural method of the little algae of claim 1 described ocean produce oil is characterized in that, little algae culture condition is: culture temperature is 23～27 ℃, continuous illumination and ventilation, and intensity of illumination is 60～100 μ mol m
-2s
-1, Ventilation Rate is 0.1～0.3L/min.
5. the cultural method of the little algae of claim 1 described ocean produce oil is characterized in that, high gloss oil fat inductive light intensity is: 150～300 μ mol m
-2s
-1
6. the cultural method of the little algae of claim 1 described ocean produce oil, it is characterized in that, as the sanitary sewage that nutritive salt is provided is that the sanitary sewage through second-stage treatment is carried out pre-treatment again, after filtering out the large granular impurity in the sewage, add 10～100 μ l/L chlorine bleach liquores and disinfected 12～48 hours.
7. the cultural method of the little algae of claim 1 described ocean produce oil is characterized in that, used marine microalgae is little plan ball algae.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618445A (en) * | 2012-04-13 | 2012-08-01 | 暨南大学 | Method for producing microalgae grease by using eustigmatoa cf. polyphem and culture method of eustigmatoa cf. polyphem |
| CN102618446A (en) * | 2012-04-16 | 2012-08-01 | 北京昊业怡生科技有限公司 | Method for cultivating oleaginous microalgae by using fecal sewage |
| CN103881923A (en) * | 2012-12-21 | 2014-06-25 | 中国中化股份有限公司 | Method for culturing microalgae by using coking wastewater |
| CN106434778A (en) * | 2016-12-05 | 2017-02-22 | 新奥科技发展有限公司 | Method for producing grease from microalgae |
| CN110229755A (en) * | 2019-06-17 | 2019-09-13 | 昆明理工大学 | A method of promote bloom nitrogen stress to coerce lower microalgae grease using epiphysin and accumulates |
| CN113481141A (en) * | 2021-08-04 | 2021-10-08 | 华东理工大学 | Method for directionally culturing and enriching high-value microalgae biomass by coupling sludge extracting solution with CO2 and device for directionally culturing microalgae |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618445A (en) * | 2012-04-13 | 2012-08-01 | 暨南大学 | Method for producing microalgae grease by using eustigmatoa cf. polyphem and culture method of eustigmatoa cf. polyphem |
| CN102618446A (en) * | 2012-04-16 | 2012-08-01 | 北京昊业怡生科技有限公司 | Method for cultivating oleaginous microalgae by using fecal sewage |
| CN103881923A (en) * | 2012-12-21 | 2014-06-25 | 中国中化股份有限公司 | Method for culturing microalgae by using coking wastewater |
| CN106434778A (en) * | 2016-12-05 | 2017-02-22 | 新奥科技发展有限公司 | Method for producing grease from microalgae |
| CN106434778B (en) * | 2016-12-05 | 2019-09-20 | 新奥科技发展有限公司 | A kind of method of microalgae production grease |
| CN110229755A (en) * | 2019-06-17 | 2019-09-13 | 昆明理工大学 | A method of promote bloom nitrogen stress to coerce lower microalgae grease using epiphysin and accumulates |
| CN113481141A (en) * | 2021-08-04 | 2021-10-08 | 华东理工大学 | Method for directionally culturing and enriching high-value microalgae biomass by coupling sludge extracting solution with CO2 and device for directionally culturing microalgae |
| CN113481141B (en) * | 2021-08-04 | 2024-02-13 | 华东理工大学 | Method for directionally culturing and enriching high-value microalgae biomass by coupling sludge extracting solution with CO2 and directional microalgae culturing device |
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Application publication date: 20110921 |