CN103881923A - Method for culturing microalgae by using coking wastewater - Google Patents
Method for culturing microalgae by using coking wastewater Download PDFInfo
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- CN103881923A CN103881923A CN201210564611.XA CN201210564611A CN103881923A CN 103881923 A CN103881923 A CN 103881923A CN 201210564611 A CN201210564611 A CN 201210564611A CN 103881923 A CN103881923 A CN 103881923A
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Abstract
The present invention relates to a method for culturing microalgae by using coking wastewater, wherein a proper amount of coking wastewater is added to a microalgae culture medium, and the microalgae cells are cultured until achieving a stable growth stage so as to harvest the microalgae. According to the present invention, the results show that the growth rate of the microalgae in seawater added with the coking wastewater is higher than the growth rate of the microalgae only in seawater, and the total nitrogen of the coking wastewater is effectively degraded; and the microalgae culture and the coking wastewater treatment are combined, such that the microalgae production cost can be effectively reduced, the coking wastewater treatment environmental benefits are achieved while the microalgae is cultured, the microalgae industrial culture cost is easily reduced, and the algae bio-energy industrialization is promoted.
Description
Technical field
The invention belongs to micro-algae bioenergy field, relate to micro-algae culture technique, be specifically related to a kind of method of utilizing coking chemical waste water to cultivate micro-algae.
Background technology
In recent years, along with the rapid growth of global economy, the consumption of the fossil energies such as oil and coal is significantly risen, and fossil energy shortage crisis is extremely urgent, and the concern of the renewable energy sources such as biomass energy is gradually become to focus.In numerous non-grain biomass, that algae has is widely distributed, fat content is high, adaptive capacity to environment is strong, growth cycle is short, output high, prepares biofuel have development prospect with algae.Carbonic acid gas is as a kind of greenhouse gases, after Kyoto Protocol signature comes into force, be subject in the world emission limit set, how reducing emission of carbon dioxide it is carried out to recycling also become study hotspot, algae can be fixed great amount of carbon dioxide in culturing process, and this solution for this global problem of carbon dioxide discharge-reduction has important value.
Utilize on a large scale micro-algae to prepare biofuel, must there is sufficient micro-algal biomass source, microalgae mass is cultivated becomes one of key link of micro-algae Biological Energy Industry industrialization, how to reduce the acquisition cost of micro-algal biomass, has become the study hotspot of micro-algae bioenergy technology.At present, have Duo Jia universities and colleges, R&D institution and enterprise both at home and abroad and set foot in micro-algae bioenergy field, reducing micro-algae cultivation cost is a problem demanding prompt solution all the time, there are some researches show at present, micro-algae can grow in sanitary sewage and organic waste water, as patent CN101525576A, a kind of method of utilizing sanitary wastewater and trade effluent to produce micro-algae is disclosed; Micro-algae can also utilize biogas fermentation waste liquid-natural pond liquid to cultivate.Patent CN101285075A, discloses a kind of biogas fermentation and autotrophy freshwater microalgae and cultivates coupling process; The concentrated of micro-algae can reach separating of micro-algae and moisture by getting rid of by siphon after the moisture filter in micro-algae liquid and collecting moisture with collecting.Patent CN101693878A, discloses the method and apparatus that a kind of micro-algae is concentrated and collect; Separately change photoreactor material to reduce costs.Patent CN101914431A, the apparatus and method that disclose a kind of utilizing all plastic modular photobioreactor system and cultivate micro-algae.Mainly ultraviolet mutagenesis to micro-algae high-performance Stains filament method, as the micro-algae of the high fat in ocean that Xiamen University's Master's thesis is prepared based on biofuel is screened.Solving micro-algae cultivates expensive key to the issue and is to improve microalgae grease content and micro-algae is carried out high-density culture or reduces the cost of material in micro-algae culturing process or improve culture technique, above-mentioned patent is Material Cost or improvement algae kind angle and then reduce micro-algae and cultivate cost from reduce micro-algae culturing process substantially, the two is not combined.The method of rationally utilizing coking chemical waste water to cultivate the micro-algae of autotrophy seawater both at home and abroad at present is still in the blank stage.
Summary of the invention
The object of the invention is to utilize coking chemical waste water to cultivate micro-algae, utilize on the one hand coking chemical waste water to reduce Material Cost, on the other hand micro-algae is carried out to high-density culture, improve micro algae growth speed, reach the biological degradation effect to coking chemical waste water simultaneously.
In order to achieve the above object, technical scheme of the present invention is:
A kind of method of utilizing coking chemical waste water to cultivate micro-algae, in the seawater that contains micro-algae algae kind, add the coking chemical waste water that accounts for seawater volume 6%-20%, under illumination, logical carbonic acid gas and air conditions, cultivate, be cultured to micro algae growth and enter stationary phase, centrifugation results frond.Described micro algae growth refers to the stage that the frustule concentration of micro-algae no longer increases stationary phase.
Concrete culture condition of the present invention is: culture temperature 18-30 DEG C, pH value 7.5-8.4, carbonic acid gas intake are that 4ml/min – 20ml/min, air intake are 0.3vvm – 0.9vvm, illumination 4000lx-10000lx, Light To Dark Ratio 12h:12h-14h:10h, while being cultured to microalgae cell growth stationary phase, adopt 8000rpm – 10000rpm, the centrifugal results frond of 10min – 20min.
Coking chemical waste water of the present invention is the water outlet of Coal Chemical Industry plant effluent or petrochemical industry chemical plant biological treatment anaerobic treatment section, and waste water COD is 900mg/L-3000mg/L, and total nitrogen is 100mg/L-180mg/L, and pH is 7.0-8.6; Described seawater is natural sea-water, artificial seawater or both mixing, and salinity is 2.0%-3.9%.
Illumination of the present invention is fluorescent lamp illumination, Xenon light shining or natural light.
The reactor that micro-algae of the present invention is cultivated is tubular type bioreactor, unlimited pond formula photoreactor, the closed photoreactor of column or board-like sealing photoreactor.
The present invention can adopt chloroform/methanol (2:1, v:v)-extracting method or other conventional meanses to measure fat content by micro-algae of separated and collected.
The mentioned micro-algae of the present invention is seawater Zhanjiang Isochrysis galbana, chlorella or flat algae, all has commercially available.
The present invention contains micro algae growth and the necessary nutritive substance of oil and fat accumulation and trace element by utilizing in coking chemical waste water, as carbon, nitrogen and other trace elements, improve micro algae growth speed, thereby realize micro-algae of the high fat content of continuous production, reach the biological treatment to coking chemical waste water simultaneously, be conducive to reduce the industrialization cultivation cost of the micro-algae of the energy.
Embodiment
The following examples are used for further describing the present invention, but do not form limitation of the invention.The coking chemical waste water that the present embodiment adopts is that this waste water COD is 940mg/L from certain coal plant's coking chemical waste water biological treatment anaerobism section water outlet, and total nitrogen is 111.8mg/L, and pH is 8.21; Seawater used Zhanjiang Isochrysis galbana is from Dalian Chemistry and Physics Institute algae kind storehouse.
Embodiment
In 800ml tubular type bioreactor, adding algae cell density is seawater Zhanjiang Isochrysis galbana nutrient solution (artificial seawater, salinity is 2.6%) 282ml of 8,000,000/ml, then adds the coking chemical waste water of 18ml, and cumulative volume is 300ml; Control sample is for utilizing artificial seawater to cultivate, and salinity is 2.6%; Coking chemical waste water is cultivated 3 samples.Culture condition is: 25 DEG C, pH value 8.2, carbonic acid gas 8mL/min, air 0.4vvm, fluorescent lamp irradiation, illumination 10000lx, Light To Dark Ratio 14h:10h.Be cultured to the 13rd day frond and enter stationary phase, centrifugal collection frond, adopts chloroform/methanol (2:1, v:v) method to extract frond grease to the frond of collecting, and carries out fat content analysis.
Experimental result shows, the growth velocity of micro-algae of cultivating in coking chemical waste water is apparently higher than micro-algae of cultivating in seawater, and in coking chemical waste water, the frustule concentration of micro-algae is 116% in seawater.In addition, micro-algae frond fat content that coking chemical waste water and seawater obtain is all higher, and the microalgae grease content that coking chemical waste water is cultivated is 38.93%, and the microalgae grease content of cultivating in seawater is 36.35%, and the two is basically identical.Total nitrogen degradation situation that three coking chemical waste waters in embodiment are cultivated to micro-algae sample is tested, and total nitrogen concentration is measured and adopted GB-11894-89 method, and result shows, the degradation rate average out to 28.16% of micro-algae to coking chemical waste water total nitrogen.
Claims (7)
1. a method of utilizing coking chemical waste water to cultivate micro-algae, it is characterized in that: add to containing in micro-algae algae kind seawater the coking chemical waste water that accounts for seawater volume 6%-20%, under illumination, logical carbonic acid gas and air conditions, cultivate, be cultured to micro algae growth and enter stationary phase, centrifugation results frond.
2. the method for utilizing coking chemical waste water to cultivate micro-algae as claimed in claim 1, is characterized in that: be that 4ml/min – 20ml/min, air intake are to carry out micro-algae cultivation under 0.3vvm – 0.9vvm, illumination 4000lx-10000lx, Light To Dark Ratio 12h:12h-14h:10h condition at temperature 18-30 DEG C, pH value 7.5-8.4, carbonic acid gas intake.
3. the method for utilizing coking chemical waste water to cultivate micro-algae as claimed in claim 1, is characterized in that: described coking chemical waste water COD is 900mg/L-3000mg/L, and total nitrogen is 100mg/L-180mg/L, and pH is 7.0-8.6.
4. the method for utilizing coking chemical waste water to cultivate micro-algae as claimed in claim 1, is characterized in that: described seawater is natural sea-water, artificial seawater or both mixing, and salinity is 2.0%-3.9%.
5. the method for utilizing coking chemical waste water to cultivate micro-algae as claimed in claim 1, is characterized in that: described illumination is fluorescent lamp illumination, Xenon light shining or natural light.
6. the method for utilizing coking chemical waste water to cultivate micro-algae as claimed in claim 1, is characterized in that: the reactor that described micro-algae is cultivated is tubular type bioreactor, unlimited pond formula photoreactor, the closed photoreactor of column or board-like sealing photoreactor.
7. the method for utilizing coking chemical waste water to cultivate micro-algae as claimed in claim 1, is characterized in that: described micro-algae is seawater Zhanjiang Isochrysis galbana, chlorella or flat algae.
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Cited By (7)
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| CN104789478A (en) * | 2015-04-24 | 2015-07-22 | 云南绿A生物产业园有限公司 | Culture method and culture system of haematococcus pluvialis species |
| CN104862231A (en) * | 2015-06-12 | 2015-08-26 | 江南大学 | Method for culturing nitrogen fixation spirulina by using kitchen wastewater |
| CN105861312A (en) * | 2016-05-13 | 2016-08-17 | 山东大学 | Method for culturing microalgae by adding anaerobic digestion liquid of kitchen waste to natural seawater |
| CN105969664A (en) * | 2016-05-13 | 2016-09-28 | 山东大学 | Method for promoting oil accumulation of microalgae by adding high-concentration organic wastewater into natural seawater |
| CN106754390A (en) * | 2016-12-30 | 2017-05-31 | 山东宝来利来生物工程股份有限公司 | The albuminiferous chlorella of one plant height and its cultural method and application |
| CN104250617B (en) * | 2014-09-16 | 2017-12-19 | 新奥科技发展有限公司 | A kind of both culturing microalgae method |
| ES2843634A1 (en) * | 2020-01-17 | 2021-07-19 | Univ De Las Palmas De Gran Canaria | METHOD TO PRODUCE BIOMASS FROM A MICROALGAE (Machine-translation by Google Translate, not legally binding) |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104250617B (en) * | 2014-09-16 | 2017-12-19 | 新奥科技发展有限公司 | A kind of both culturing microalgae method |
| CN104789478A (en) * | 2015-04-24 | 2015-07-22 | 云南绿A生物产业园有限公司 | Culture method and culture system of haematococcus pluvialis species |
| CN104789478B (en) * | 2015-04-24 | 2018-01-05 | 云南绿A生物工程有限公司 | A kind of cultural method and culture systems of haematococcus pluvialis algae kind |
| CN104862231A (en) * | 2015-06-12 | 2015-08-26 | 江南大学 | Method for culturing nitrogen fixation spirulina by using kitchen wastewater |
| CN105861312A (en) * | 2016-05-13 | 2016-08-17 | 山东大学 | Method for culturing microalgae by adding anaerobic digestion liquid of kitchen waste to natural seawater |
| CN105969664A (en) * | 2016-05-13 | 2016-09-28 | 山东大学 | Method for promoting oil accumulation of microalgae by adding high-concentration organic wastewater into natural seawater |
| CN105861312B (en) * | 2016-05-13 | 2019-12-31 | 山东大学 | Method for culturing microalgae by adding kitchen waste anaerobic digestion solution into natural seawater |
| CN105969664B (en) * | 2016-05-13 | 2020-09-11 | 山东大学 | Method for promoting microalgae grease accumulation by adding high-concentration organic wastewater into natural seawater |
| CN106754390A (en) * | 2016-12-30 | 2017-05-31 | 山东宝来利来生物工程股份有限公司 | The albuminiferous chlorella of one plant height and its cultural method and application |
| CN106754390B (en) * | 2016-12-30 | 2019-07-26 | 山东宝来利来生物工程股份有限公司 | The albuminiferous chlorella of one plant height and its cultural method and application |
| ES2843634A1 (en) * | 2020-01-17 | 2021-07-19 | Univ De Las Palmas De Gran Canaria | METHOD TO PRODUCE BIOMASS FROM A MICROALGAE (Machine-translation by Google Translate, not legally binding) |
| WO2021144491A1 (en) * | 2020-01-17 | 2021-07-22 | Universidad De Las Palmas De Gran Canaria | Method for producing biomass from a microalga |
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Application publication date: 20140625 |