CN104762331A - Method and culture device for coupling biogas fermentation with microalgae culture - Google Patents
Method and culture device for coupling biogas fermentation with microalgae culture Download PDFInfo
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- CN104762331A CN104762331A CN201510148577.1A CN201510148577A CN104762331A CN 104762331 A CN104762331 A CN 104762331A CN 201510148577 A CN201510148577 A CN 201510148577A CN 104762331 A CN104762331 A CN 104762331A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
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Abstract
The invention discloses a method and a culture device for coupling biogas fermentation with microalgae culture. The method comprises the following steps: carrying out biogas fermentation to obtain biogas and biogas slurry; filtering the biogas slurry to remove solid granules, diluting the biogas slurry to serve as a microalgae culture medium, filling the diluted biogas slurry in a microalgae culture reactor to serve as the microalgae culture medium, filling the bottom of the microalgae culture solution in the microalgae culture reactor with biogas to serve as a carbon source, culturing under a light condition, and drying and removing water vapor from the biogas flowing out from the microalgae culture reactor for use; when harvesting the microalgae, dewatering to obtain microalgae sludge and culture solution, extracting biolipid, phycocyanin and other products with high added values from the microalgae sludge, and carrying out biogas fermentation on the residual microalgae residues and the culture solution in a biogas digester as biogas fermentation materials. The entire process of the method disclosed by the invention is driven by the solar energy, and carbon, nitrogen, phosphorus and other microelements are recycled, thereby satisfying the requirements of green energy source and sustainable development.
Description
Technical field:
The invention belongs to field of microalgae cultivation, be specifically related to a kind of method of being coupled with microdisk electrode by biogas fermentation and culture apparatus.
Background technology:
Micro-algae, as a kind of original autotrophic type unicellular organism, compares with high green plants, has high reproduction speed and photosynthetic efficiency.Do not occupy cultivated land with it, the advantages such as water quality requirement is low, become third generation bioenergy raw material after corn simultaneously.Especially the oil-rich microalgae of fat content more than 40% algae kind Screening germplasm and develop and day by day receive the concern of people.Meanwhile, one slightly algae have the Phycocyanins, C-of high content, the high value-added product such as astaxanthin.Therefore the energy and large-scale developing and utilizing of the micro-algae of high added value resource have fabulous development prospect.
Current high cost is the important factor hindering micro-algae industry extensive development.These costs comprise screening and the child care of high-quality algae kind resource, microdisk electrode cost, microalgae recovery cost, product extraction and subsequent machining cost etc.Wherein microdisk electrode cost accounts for integral part in whole industrial chain cost.Therefore it is all vital for finding the continuable training mode of a kind of efficient energy-saving environmental protection to reducing micro-algae production cost and even whole micro-algae energy and other product industrial chains.
Along with the raising of national awareness of environment protection, the ight soil etc. of crop waste stalk, discarded firewood and poultry livestock breeding is used to biogas fermentation more.Decrease on the one hand agricultural crop straw directly to burn the topsoil and the wasting of resources that cause, the utilization of poultry livestock breeding ight soil also prevent the water pollution of directly discharging and causing on the other hand.But the product of biogas fermentation comprises biogas, natural pond liquid and natural pond slag three part.Except biogas, also containing a large amount of nitrogen phosphorus and other trace elements in the liquid of natural pond, there is very high utility value.And at present mostly directly discharge or field irrigation are to the process of natural pond liquid.Such processing mode not only can cause soil compaction also can cause other environmental problems such as groundwater pollution.
Summary of the invention:
First object of the present invention is to provide a kind of method be coupled with microdisk electrode by biogas fermentation.
The method be coupled with microdisk electrode by biogas fermentation of the present invention, is characterized in that, comprise the following steps:
A, biogas fermentation obtain biogas and natural pond liquid;
B, natural pond liquid removes solid particulate after filtration, as the substratum of micro-algae after dilution, load in microdisk electrode reactor, as the substratum of microdisk electrode, biogas passes into the bottom of the micro algae culturing liquid in microdisk electrode reactor as carbon source, cultivate under illumination condition, containing methane in biogas, outside the inflammable gas such as carbon monoxide and nitrogen protoxide, carbonic acid gas also containing 50-70%, these nitrogen peroxide are incombustible and can reduce the quality of biogas, using the carbonic acid gas in biogas as carbon source, micro-algae is utilized to remove carbonic acid gas, not only for micro algae growth provides carbon source also purifying biogas, carbonic acid gas is removed from microdisk electrode reactor biogas out, drying remove after water vapour for, as for burning, the purposes such as illumination,
C, to microalgae harvesting time, algae mud and nutrient solution is obtained through processed, algae mud carries out the extraction of bio-oil, Phycocyanins, C-and other high value added products, and after extracting, remaining algae-residue and nutrient solution enter in methane-generating pit as biogas fermentation material and carry out biogas fermentation.
Described processed can be through air supporting or/and flocculation obtains algae mud and nutrient solution.
Biogas fermentation is for the production of biogas, and solve crop waste stalk, poultry livestock breeding and micro-algae put forward the process problem of oil residue simultaneously, and fermentation gained natural pond liquid also can enter microalgae culture system.
Described natural pond liquid removes solid particulate after filtration, as the substratum of micro-algae after dilution, the Dilution ratio of natural pond liquid with at that time when thing natural pond liquid and determine.Because different fermentation raw materials, fermentation time, even fermentative microflora all can have an impact to the nitrogen and phosphorus content in the liquid of natural pond, meanwhile, micro-algae of different genera is also had nothing in common with each other to the nitrogen phosphorus absolute content in substratum and ratio, numerous.
From microdisk electrode reactor biogas out due to containing a small amount of water vapour, after simple drying, therefore namely can be used as high-quality marsh gas for purposes such as burning and illuminations.
Natural pond liquid provides nitrogenous source, phosphorus source and other trace elements, and the carbonic acid gas in biogas, as carbon source, is that the energy is cultivated with sun power.Meanwhile, the passing into the disturbance caused nutrition can be made fully to mix of biogas, light source is also fully used, and extra power need not be provided to stir.
After extracting, remaining algae-residue is rich in C, the multiple elements such as N, P, and belongs to broken cell residue, very easily degradable fermented, therefore can enter methane-generating pit fermentation as the biogas fermentation material of high-quality.
Second object of the present invention is to provide a kind of culture apparatus be coupled with microdisk electrode by biogas fermentation, it is characterized in that, comprise methane fermentation system, filtering system, microdisk electrode reactor, biofuel extractor, moisture trap, the bottom of methane fermentation system is connected with the entrance of filtering system through natural pond liquid transport pipe, the outlet of filtering system is connected with microdisk electrode reactor through nutrient solution transport pipe, the solid discharge of filtering system is connected with the top of methane fermentation system through residue transport pipe, biogas transport pipe is provided with on the top of methane fermentation system, this biogas transport pipe passes into the bottom of microdisk electrode reactor, biogas is except providing carbonic acid gas for except micro algae growth as carbon source, also can agitate algae liquid, play the effect of stirring, be provided with purifying biogas transport pipe to be connected with moisture trap on the top of microdisk electrode reactor, moisture trap uses equipment to be connected through biogas utilization pipeline and biogas again, be connected with biofuel extractor through algae liquid transport pipe in the bottom of microdisk electrode reactor, the oil residue outlet of carrying of biofuel extractor is connected with methane fermentation system through algae-residue transport pipe, microdisk electrode reactor top is also connected with diluting water pipeline.
Preferably, natural pond liquid transport pipe, residue transport pipe, biogas transport pipe, algae-residue transport pipe, diluting water pipeline, purifying biogas transport pipe, algae liquid transport pipe, biogas utilization pipeline and nutrient solution transport pipe are all provided with valve, with the flowing of convenient control gas and liquid.
The movement of poultry livestock and waste plant straws, firewood etc. produce biogas, natural pond liquid and natural pond slag after microbiological anaerobic fermentation.Wherein biogas is mixed gas, and general containing methane 50 ~ 70%, all the other are carbonic acid gas and a small amount of nitrogen, hydrogen and hydrogen sulfide etc.The nutritive element such as nitrogen, phosphorus and various trace elements is rich in the liquid of natural pond.The present invention utilizes the carbonic acid gas in biogas to substitute carbon source in microdisk electrode, and biogas gas mixture removes methane content after carbonic acid gas through algae liquid can reach more than 90%, and through dewatering, drying is high-quality marsh gas, can be used as other purposes such as fuel and illumination.Utilize natural pond liquid as micro-algae culture medium, the nitrogen in the liquid of natural pond, phosphorus and trace element substitute other nutritive elements in microdisk electrode, significantly can reduce the production cost of microdisk electrode.Nutrient solution after microalgae recovery and the algae-residue of micro-algae after grease and biomass extract enter methane-generating pit and carry out circulating fermentation.Namely the grease extracted obtains biofuel after turn esterified.Whole process drives with solar energy, and carbon, nitrogen, phosphorus and other Microelemental cycles utilize, and meet green energy resource and Sustainable development requirement.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of the culture apparatus be coupled with microdisk electrode by biogas fermentation of the present invention;
Wherein 1, methane fermentation system; 2, filtering system; 3, microdisk electrode reactor; 4, biofuel extractor; 5, moisture trap; 6, biogas lamp; 7, natural pond liquid transport pipe; 8, residue transport pipe; 9, biogas transport pipe; 10, algae-residue transport pipe; 11, diluting water pipeline; 12, purifying biogas transport pipe; 13, algae liquid transport pipe; 14, biogas utilization pipeline; 15, nutrient solution transport pipe; A, B, C, D, E, F, G, H, I, J, K, L, M and N represent valve A, valve B, valve C, valve D, valve E, valve F, valve G, valve H, valve I, valve J, valve K, valve L, valve M and valve N respectively.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
As shown in Figure 1, the culture apparatus that biogas fermentation is coupled with microdisk electrode of the present embodiment, comprise methane fermentation system 1, filtering system 2, microdisk electrode reactor 3, biofuel extractor 4, moisture trap 5, the bottom of methane fermentation system 1 is connected with the entrance of filtering system 2 through natural pond liquid transport pipe 7, the outlet of filtering system is connected with microdisk electrode reactor 3 through nutrient solution transport pipe 15, the solid discharge of filtering system is connected with the top of methane fermentation system through residue transport pipe 8, biogas transport pipe 9 is provided with on the top of methane fermentation system, this biogas transport pipe passes into the bottom of microdisk electrode reactor 3, be provided with purifying biogas transport pipe 12 to be connected with moisture trap 5 on the top of microdisk electrode reactor, moisture trap uses equipment (the present embodiment is biogas lamp 6) to be connected through biogas utilization pipeline 14 and biogas again, be connected with biofuel extractor 4 through algae liquid transport pipe 13 in the bottom of microdisk electrode reactor, the oil residue outlet of carrying of biofuel extractor is connected with methane fermentation system through algae-residue transport pipe 10, microdisk electrode reactor top is also connected with diluting water pipeline 11.
Natural pond liquid transport pipe 7, residue transport pipe 8, nutrient solution transport pipe 15 and diluting water pipeline 11 are respectively equipped with valve C, valve D, valve F and valve N.Algae-residue transport pipe 10 is sequentially with valve A and valve K, biogas transport pipe 9 is sequentially with valve B and valve E, purifying biogas transport pipe 12 is sequentially with valve G and valve H, biogas utilization pipeline 14 is sequentially with valve I and valve J, algae liquid transport pipe 13 is sequentially with valve L and valve M.
Micro-algae that the present embodiment uses is grid algae Scenedesmus sp..Put into methane fermentation system 1 using the movement of poultry livestock and waste plant straws, firewood etc. as biogas fermentation material and produce biogas, natural pond liquid and natural pond slag after microbiological anaerobic fermentation, open valve C, D and F, natural pond liquid enters in filtering system 2 through natural pond liquid transport pipe 7, natural pond liquid after filtration enters in microdisk electrode reactor 3 as substratum through nutrient solution transport pipe 15, and macrobead solid and filter residue return in methane fermentation system through residue transport pipe 8.Because grid algae Scenedesmus sp. is seawater algae, therefore add for what dilute natural pond liquid the mixture that water is tap water and seawater, salinity is 25 ‰, if the algae kind of cultivating is algae, directly adds tap water.Open valve N, add water and added by diluting water pipeline 11 and enter in microdisk electrode reactor 3, the natural pond liquid after filtration adds in microdisk electrode reactor with the form that stream adds, and the volume of microdisk electrode reactor is 100L.Open valve B and valve E, biogas in methane fermentation system inputs the bottom of microdisk electrode reactor through biogas transport pipe 9, cultivate under sunlight illumination condition, in culturing process in microdisk electrode reactor Ventilation Rate with overflow biogas in not carbonated be as the criterion, by Valve controlling, cultivate in earlier stage because algae liquid concentration is low, dilution carbonic acid gas speed is less, suitably should turn down airflow rate, late stage of culture is corresponding increase then.In the present embodiment, airflow rate is between 5-20vvm.Pass in the biogas of microdisk electrode reactor containing methane, outside the inflammable gas such as carbon monoxide and nitrogen protoxide, carbonic acid gas also containing 50-70%, these nitrogen peroxide are incombustible and can reduce the quality of biogas, using the carbonic acid gas in biogas as carbon source, micro-algae is utilized to remove carbonic acid gas, not only for micro algae growth provides carbon source also purifying biogas, open valve G, H, I and J, carbonic acid gas is removed from microdisk electrode reactor biogas out, enter purifying biogas transport pipe 12 again, water vapour is removed through moisture trap 5, pass into biogas utilization pipeline 14, award biogas lamp 6 illumination to use.
Cultivate micro-algae algae liquid concentration after 5 days to increase, OD
680when reaching 1, natural pond liquid can be closed and enter valve F, biogas enters valve E, valve N is entered with diluting water, algae liquid is allowed to leave standstill after 12 hours, open valve M, valve L, valve A and valve K, the concentrated algae liquid of microdisk electrode reactor bottom delivers in biofuel extractor 4 through algae liquid transport pipe 13, carry out processed wherein, obtain algae mud and nutrient solution, algae mud, through cytoclasis, obtains bio-oil after carrying oil, carries the algae-residue after oil and nutrient solution and enters in methane fermentation system 1 through algae-residue transport pipe 10 and ferment.Gather 90% of whole micro algae culturing liquid volume, residue 10% cultivates algae kind for continuing at every turn.
Claims (4)
1. by the method that biogas fermentation is coupled with microdisk electrode, it is characterized in that, comprise the following steps:
A, biogas fermentation obtain biogas and natural pond liquid;
B, natural pond liquid remove solid particulate after filtration, as the substratum of micro-algae after dilution, load in microdisk electrode reactor, as the substratum of microdisk electrode, biogas passes into the bottom of the micro algae culturing liquid in microdisk electrode reactor as carbon source, cultivate under illumination condition, microdisk electrode reactor biogas drying out remove after water vapour for;
C, to microalgae harvesting time, algae mud and nutrient solution is obtained through processed, algae mud carries out the extraction of bio-oil, Phycocyanins, C-and other high value added products, and after extracting, remaining algae-residue and nutrient solution enter in methane-generating pit as biogas fermentation material and carry out biogas fermentation.
2. method according to claim 1, is characterized in that, described processed is through air supporting or/and flocculation obtains algae mud and nutrient solution.
3. the culture apparatus that biogas fermentation is coupled with microdisk electrode, it is characterized in that, comprise methane fermentation system, filtering system, microdisk electrode reactor, biofuel extractor and moisture trap, the bottom of methane fermentation system is connected with the entrance of filtering system through natural pond liquid transport pipe, the outlet of filtering system is connected with microdisk electrode reactor through nutrient solution transport pipe, the solid discharge of filtering system is connected with the top of methane fermentation system through residue transport pipe, biogas transport pipe is provided with on the top of methane fermentation system, this biogas transport pipe passes into the bottom of microdisk electrode reactor, be provided with purifying biogas transport pipe to be connected with moisture trap on the top of microdisk electrode reactor, moisture trap uses equipment to be connected through biogas utilization pipeline and biogas again, be connected with biofuel extractor through algae liquid transport pipe in the bottom of microdisk electrode reactor, the oil residue outlet of carrying of biofuel extractor is connected with methane fermentation system through algae-residue transport pipe, microdisk electrode reactor top is also connected with diluting water pipeline.
4. the culture apparatus that biogas fermentation is coupled with microdisk electrode according to claim 3, it is characterized in that, natural pond liquid transport pipe, residue transport pipe, biogas transport pipe, algae-residue transport pipe, diluting water pipeline, purifying biogas transport pipe, algae liquid transport pipe, biogas utilization pipeline and nutrient solution transport pipe are all provided with valve.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105603019A (en) * | 2016-01-29 | 2016-05-25 | 厦门大学 | Method for accumulating carbohydrates by coupling microalgae with biogas slurry |
| CN106906270A (en) * | 2017-04-12 | 2017-06-30 | 郭雨汇 | Waste water and the method for producing carotenoid, gaseous fuel and organic fertilizer are processed using microalgae |
| CN106927635A (en) * | 2017-04-11 | 2017-07-07 | 大连理工大学 | Energy output type sewage treatment process |
| CN107142197A (en) * | 2017-06-23 | 2017-09-08 | 四川省天惠能源科技有限公司 | A kind of biogas microalgae joint ecological treatment system and its application |
| CN107151055A (en) * | 2017-06-01 | 2017-09-12 | 中国农业大学 | A kind of method that waste water of livestock poultry environment increment is utilized |
| CN108148738A (en) * | 2018-01-05 | 2018-06-12 | 厦门大学 | A kind of culture microalgae processing biogas slurry coupling anaerobic ferment devices and its operation method |
| JP2018168274A (en) * | 2017-03-29 | 2018-11-01 | 住友大阪セメント株式会社 | Biogas utilization method, biogas utilization system and biogas |
| CN110407404A (en) * | 2019-07-02 | 2019-11-05 | 叶建锋 | A kind of production method and system converting nutrients in agricultural effluent to crude protein raw material |
| CN112813113A (en) * | 2020-12-30 | 2021-05-18 | 兴源环境科技股份有限公司 | Method for producing bio-oil by livestock and poultry manure synergism |
| CN113072177A (en) * | 2021-03-10 | 2021-07-06 | 南京理工大学 | Device and method for synchronously treating sludge digestive fluid and biogas by using in-situ algae |
| CN113800722A (en) * | 2021-10-09 | 2021-12-17 | 沈阳航空航天大学 | Method for co-producing methane-bio-oil by coupling domestic sewage anaerobic digestion with microalgae culture |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105603019A (en) * | 2016-01-29 | 2016-05-25 | 厦门大学 | Method for accumulating carbohydrates by coupling microalgae with biogas slurry |
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| CN110407404A (en) * | 2019-07-02 | 2019-11-05 | 叶建锋 | A kind of production method and system converting nutrients in agricultural effluent to crude protein raw material |
| CN112813113A (en) * | 2020-12-30 | 2021-05-18 | 兴源环境科技股份有限公司 | Method for producing bio-oil by livestock and poultry manure synergism |
| CN113072177A (en) * | 2021-03-10 | 2021-07-06 | 南京理工大学 | Device and method for synchronously treating sludge digestive fluid and biogas by using in-situ algae |
| CN113072177B (en) * | 2021-03-10 | 2022-12-09 | 南京理工大学 | Device and method for synchronously treating sludge digestive fluid and biogas by using in-situ algae |
| CN113800722A (en) * | 2021-10-09 | 2021-12-17 | 沈阳航空航天大学 | Method for co-producing methane-bio-oil by coupling domestic sewage anaerobic digestion with microalgae culture |
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