CN103087920B - Mixotrophic scenedesmus and application thereof in sewage resource treatment - Google Patents
Mixotrophic scenedesmus and application thereof in sewage resource treatment Download PDFInfo
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- CN103087920B CN103087920B CN201310027975.9A CN201310027975A CN103087920B CN 103087920 B CN103087920 B CN 103087920B CN 201310027975 A CN201310027975 A CN 201310027975A CN 103087920 B CN103087920 B CN 103087920B
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- scenedesmus
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Abstract
The invention relates to mixotrophic scenedesmus and an application in sewage resource treatment. The scenedesmus has strong anti-pollution capability and can grow stably in actual sewage (non-sterile), the heterotrophic capability is strong, the productivity of biomass and grease is high, namely organic matters in the sewage can be transformed to grease, nutrient substances in the sewage can be further utilized for absorbing carbon dioxide in atmosphere, nitrogen and phosphorus in the sewage can be removed in a sustained manner under the situation that an organic carbon source is used up, and when the sewage is taken as a matrix for inoculating the scenedesmus, the water quality can be purified, and the grease can be simultaneously accumulated, so that sewage resources can be effectively utilized, the waste can be changed to valuable things, and the mixotrophic scenedesmus further has the advantages of low operation cost, good quality of outlet water, simple and convenient technology, easiness in popularization and the like.
Description
Technical field
The present invention relates to water pollution control and production of biodiesel field, be specifically related to a strain mixotrophism type grid algae and the application in sewage recycling is processed thereof.
Background technology
In water pollution control field, the conventional treatment process taking activated sludge process as representative is difficult to realize the recycling to sewage, and the organism in sewage and inorganic salt run off with the form of gas or mud with the processing of sewage, the present situation of having aggravated resource scarcity.In production of biodiesel field, micro-algae is cultivated the key constraints that high cost is the micro-algae biodiesel commercialization of restriction, cultivate micro-algae taking sewage as matrix and can provide cheap biomass material as the production of biofuel and allogenic material product thereof, thereby significantly reduce production costs.A little less than common high produce oil algae kind contamination resistance, be difficult to stable existence in the actual sewage of sterilized processing, seriously limited the commercial applications of the micro-algae culture technique based on sewage.Screening adaptation Sewage Environment, the suitable algae kind that oil offtake is high, pollutant removal is good, significant for commercially producing of micro-algal biomass product such as the recycling and the biofuel etc. that realize sewage.
Summary of the invention
In order to overcome the shortcoming of above-mentioned prior art, the object of this invention is to provide a strain mixotrophism type grid algae and the application in sewage recycling is processed thereof, this micro-algae has advantages of the Sewage Environment of adaptation, oil offtake is high, pollutant removal good.
In order to achieve the above object, the technical scheme that the present invention takes is:
One strain mixotrophism type grid algae, grid algae (Scenedesmus sp.ZTY1) separates from sanitary sewage disposal factory of Beijing, be preserved in China Microbial Culture Preservation Commission's common micro-organisms center (CGMCC) on January 14th, 2013, register on the books and be numbered 7059, CGMCC address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode is 100101.
Described grid algae (Scenedesmus sp.ZTY1) coenobium is flat, is made up of 2 or 4 cells, and colony's cell is listed on straight line, cell ovalize, cell walls is level and smooth, wide 15～25 μ m of colony of 4 cells, individual cells is wide is 3～6 μ m, long 9～12 μ m.
The application of one strain mixotrophism type grid algae in sewage recycling is processed, is first inoculated into this grid algae in sewage, removes the nutritive element such as organism and nitrogen phosphorus in sewage by the assimilation of frustule, it is characterized in that, concrete steps are:
1) in sewage, inoculate grid algae (Scenedesmus sp.ZTY1), Initial seeding density is 1 × 10
5～1 × 10
6individual/mL;
2) sewage after inoculation frustule is placed under nature or artificial lighting condition, artificial lighting condition is: Light To Dark Ratio 14:10, light intensity 800～1300lux, keep the stir speed (S.S.) of 80-150r/min and the temperature of 5～25 DEG C, make grid algae (Scenedesmus sp.ZTY1) utilize the nutrient growth in sewage;
3) cultivate centrifugal results frustule after 2～45 days, discharge simultaneously and process rear water;
4) frustule that results obtain can, for the production of biofuel or other micro-algal biomass product after further processing, also can be inoculated in new sewage and continue on for sewage treatment process.
Described sewage is the preliminary sedimentation tank water outlet of general municipal sewage plant.
Beneficial effect of the present invention is: this strain grid algae can grow in actual sewage environment, without sewage being done to sterilising treatment or additionally adding nutrition, has the ability that the organism in sewage is converted into grease, and the nitrogen phosphorus in water body can be removed to very low level.First utilize the assimilation of grid algae to absorb the nutritive element in sewage, by certain way, grid algae is separated from sewage again, obtain the sewage disposal water outlet that pollutant load is very low, thereby reduce the risk of sewage disposal plant effluent regeneration, and obtain micro-algal biomass of high fat content.The present invention can be applicable to the production of micro-algal biomass product such as sewage disposal and biofuel.
Brief description of the drawings
Fig. 1 is growth curve and the fat content variation diagram of grid algae under intermittent type culture condition (Scenedesmus sp.ZTY1) in sanitary sewage.
Fig. 2 is growth curve and the Pollutant levels variation diagram of grid algae under sequence batch (culture condition (Scenedesmus sp.ZTY1) in sanitary sewage.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the invention will be further described:
Embodiment 1
Cultivate for a long time grid algae (Scenedesmus sp.ZTY1) and can effectively improve the grease output of unit sewage in the mode of intermittently cultivating.Adopt real life sewage to carry out intermittent type cultivation to grid algae (Scenedesmus sp.ZTY1), dissolved organic carbon in sewage (DOC) concentration is 112mgL
-1, total nitrogen (TN) concentration is 41.0mgL
-1, total phosphorus (TP) concentration is 8.4mgL
-1, Initial seeding density is about 7.5 × 10
5individual mL
-1, under the condition of Light To Dark Ratio 14h:10h, 25 DEG C of temperature, intensity of illumination 800～1300lux, to cultivate after 21 days, grid algae (Scenedesmus sp.ZTY1) enters stationary phase, obtains the very low (TN:2.9mgL of carbon concentration of nitrogen and phosphorus after centrifugal results grid algae
-1, TP:0.07mgL
-1) three grades of water outlets of municipal effluent, this strain grid algae is respectively 92.9% and 99.1% to the clearance of nitrogen phosphorus in the water outlet of municipal effluent one-level.
During taking sanitary sewage as substratum, this strain grid algae (Scenedesmus sp.ZTY1) is at grease yield and the TAG(triglyceride in stationary phase early stage (cultivating 21 days), the starting material of preparing biological diesel oil) output is respectively 102mgL
-1and 32mgL
-1, grease yield and the TAG(triglyceride in later stage stationary phase (cultivating 45 days), the starting material of preparing biological diesel oil) and output is respectively 163mgL
-1and 148mgL
-1.The growth curve of grid algae under intermittent type culture condition (Scenedesmus sp.ZTY1) in sanitary sewage and fat content variation diagram are as shown in Figure 1.From growth curve chart, can find out, grid algae ZTY1 grows fine in real life sewage, and the adaptive phase is short, after about 10 days, enters stationary phase, and sewage is had to good adaptability.From grease and TAG output figure, can find out, proper extension incubation time can significantly increase the grease yield of unit sewage, promotes frustule oil and fat accumulation.
Embodiment 2
The mode high-density culture grid algae ZTY1 cultivating with sequence batch (can effectively reduce the sewage disposal time, improves processing efficiency.(DOC is that 72.9, TN is 50.4mgL to adopt real life sewage
-1, TP is 3.5mgL
-1) grid algae (Scenedesmus sp.ZTY1) is carried out to sequence batch (cultivation, culture condition is: intensity of illumination 800～1300lux, Light To Dark Ratio 14h:10h, 25 DEG C of temperature, stir speed (S.S.) 150r/min.The Initial seeding density of grid algae (Scenedesmus sp.ZTY1) is 1.28 × 10
6individual mL
-1, after each treatment cycle finishes, centrifugal draining, the frustule of results 50% is for preparing biological diesel oil, and the frustule of residue 50% is stayed in system, adds new sanitary sewage, continues the cultivation operation of next cycle.The intermittently algae variable density of grid algae (Scenedesmus sp.ZTY1) and to the removal effect of pollutent as shown in Figure 2 in culturing process, it is three days that hydraulic detention time is set, in sewage, the clearance of DOC, TN and TP can reach respectively 75%, 97% and 98%, algae cell density raises rapidly with the reduction of pollutant load in sewage, and biomass yield and grease productive rate reach as high as 181mgL
-1d
-1and 37.9mgL
-1d
-1(with dry weight basis).
Claims (1)
1. a strain mixotrophism type grid algae, is characterized in that: the deposit number of this grid algae (Scenedesmus sp.) ZTY1 is CGMCC NO.7059.
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|---|---|---|---|
| CN201310027975.9A CN103087920B (en) | 2013-01-24 | 2013-01-24 | Mixotrophic scenedesmus and application thereof in sewage resource treatment |
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| CN201310027975.9A CN103087920B (en) | 2013-01-24 | 2013-01-24 | Mixotrophic scenedesmus and application thereof in sewage resource treatment |
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| CN103087920A CN103087920A (en) | 2013-05-08 |
| CN103087920B true CN103087920B (en) | 2014-10-08 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513799A (en) * | 2013-09-29 | 2015-04-15 | 重庆工商大学 | New idea of utilizing sewage to culture oil-rich algae and also purify water |
| CN103484374B (en) * | 2013-10-14 | 2015-01-14 | 哈尔滨工业大学 | Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease |
| CN104630295B (en) * | 2015-03-08 | 2018-01-02 | 中国海洋大学 | A kind of method for handling city secondary waste water using immobilized microalgae and producing grease |
| CN108641965A (en) * | 2018-04-16 | 2018-10-12 | 天津大学 | A kind of optimization method of and foster pattern scenedesmus culture |
| CN113214996B (en) * | 2021-05-18 | 2023-03-24 | 武汉理工大学 | Scenedesmus as well as culture method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565674A (en) * | 2009-05-13 | 2009-10-28 | 清华大学 | Low-nutrition scenedesmus and application thereof in sewage deep treatment |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565674A (en) * | 2009-05-13 | 2009-10-28 | 清华大学 | Low-nutrition scenedesmus and application thereof in sewage deep treatment |
Non-Patent Citations (3)
| Title |
|---|
| 固定化的栅藻深度脱氮和除磷能力;张恩栋等;《植物生理学通讯》;20061231;第42卷(第6期);1037-1040 * |
| 安晓雯等.固定化栅藻对市政污水中氮、磷营养盐的深度净化.《中南民族大学学报(自然科学版)》.2006,第25卷(第4期),14-17. * |
| 张恩栋等.固定化的栅藻深度脱氮和除磷能力.《植物生理学通讯》.2006,第42卷(第6期),1037-1040. |
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