CN101955846A - Production method of microalgae grease based on low-carbon emission recycling of domestic sewage - Google Patents
Production method of microalgae grease based on low-carbon emission recycling of domestic sewage Download PDFInfo
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- CN101955846A CN101955846A CN2010105097024A CN201010509702A CN101955846A CN 101955846 A CN101955846 A CN 101955846A CN 2010105097024 A CN2010105097024 A CN 2010105097024A CN 201010509702 A CN201010509702 A CN 201010509702A CN 101955846 A CN101955846 A CN 101955846A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention relates to a production method of microalgae grease based on low-carbon emission recycling of domestic sewage, belonging to the field of grease production and solving the problems of low carbon recovery rate and high domestic sewage cost in the domestic sewage treatment process. The method of the invention comprises the following steps of: sterilizing primarily treated wastewater and guiding into a photobioreactor; and then, inoculating microalgae in the photobioreactor, culturing by using CO2 as an aeration air source, collecting the microalgae in effluent and dewatering to extract the grease to obtain microalgae grease. The method of the invention has the advantages of high carbon recovery rate and low grease production cost and is applied to the biodiesel field.
Description
Technical field
The invention belongs to the grease production field.
Background technology
Because biofuel has the characteristic of " carbon balance ", therefore can alleviate Global Greenhouse Effect, and its pollutant emission level is low than petrifaction diesel, so significantly improving has appearred in the whole world output of biofuel in recent years, yet cost that biofuel is high and insufficient raw material supply have hindered further applying of biofuel.In the production cost of biofuel, raw material accounts for the largest percentage, generally between 70%～80%, the suitability for industrialized production of biofuel is mainly carried out in countries in the world as raw material with vegetables oil or waste cooking oil at present, the biofuel cost of producing is not like this having under the situation of policy inclination than the petrifaction diesel height, and the market competitiveness is very low, and output is also limited, can't satisfy ever-increasing demand.Little algae is as the raw material of bioenergy, be suggested already and through experimental verification, but because the restriction of illumination, the density of frustule is lower, and the frustule particle diameter is less, therefore collects difficulty, and cost is higher, also can't be at war with fossil energy etc.
The raising of expanding economy and living standards of the people also can cause the increase of wastewater discharge, causes serious water pollution problems.China inland lake and coastland have been brought great loss not only for the aquatic products aquaculture, and have directly been threatened people health because serious " red tide " and " wawter bloom " problem also appear in eutrophication again and again.Containing abundant organism in municipal effluent or the trade effluent, traditional sewage water treatment method is under the condition of anaerobic or aerobic, oxygenizement by microorganism is converted into mineral compound to greatest extent with organic compound merely, and wherein most C is with CO
2Form be released in the atmosphere and go.Traditional sewage water treatment method, technology is simple, processing efficiency is high, and the sewage after the processing can directly be discharged in the natural water and go; But, traditional treatment process can not effectively be recycled the energy that organic compound contained in the sewage, and need carry out advanced treatment for the waste water that some contains a large amount of phosphorus and nitrogens and just can reach sewage drainage standard, in treating processes, can in atmosphere, discharge a large amount of CO simultaneously
2And CH
4Isothermal chamber gas, the rate of recovery of carbon is lower, is unfavorable for the alleviation of Greenhouse effect, thereby awaits further improvement.
At present, the low and microalgae grease cost height of the sanitary sewage disposal process carbon rate of recovery.
Summary of the invention
The objective of the invention is in order to solve the low and high problem of microalgae grease cost of the sanitary sewage disposal process carbon rate of recovery; And provide microalgae grease production method based on sanitary sewage low-carbon emission resource utilization.
Microalgae grease production method based on sanitary sewage low-carbon emission resource utilization is undertaken by following step: will handle that (one-level is handled and also to be cried pre-treatment through one-level, be by the suspension solid matter in physical methods such as precipitation, flotation, the filtration removal sewage, or by chemical processes such as cohesion, oxidation, neutralizations, make the toxic substance of strong acid, highly basic and overrich in the sewage, obtain rough purification.) waste water carry out sterilising treatment, in the lead-in light bio-reactor; In bioreactor, inoculate little algae then, with CO
2Cultivate as the aeration source of the gas, culturing process elder generation intermittent operation to little algae logarithmic growth and density reaches more than the 0.2g/L, changes continuous water inlet then into, control pH remains at 5～10 during the water inlet continuously, collect the little algae in the water outlet, after dewatering, extract grease again, promptly obtain microalgae grease.
In the present invention, utilize little algae to handle sanitary sewage, can when disposing of sewage, obtain little algal biomass, and therefrom extract the raw material of grease as biofuel, thereby lowered the production cost of microalgae grease, made full use of carbon source, reduced pollution two secondary environments.The clearance of COD, ammonia nitrogen and total phosphorus is all higher among the present invention, can satisfy the primary standard of integrated wastewater discharge standard (GB8978-1996).The anti impulsion load of the inventive method is big, is applicable to that flooding velocity changes area greatly.After the little algal biomass that produces is collected, can extract grease,, and the residuum behind the extraction grease can be carried out anaerobically fermenting and produce biogas or ethanol, really reach the resource utilization of refuse the raw materials for production of grease as biofuel.
Description of drawings
Fig. 1 is the removal effect figure of sanitary sewage COD in the embodiment 11, among the figure-◆-expression water outlet ,-■-expression water inlet ,-▲-the expression clearance; Fig. 2 is the removal effect figure of sanitary sewage total phosphorus in the embodiment 11, among the figure-◆-expression water outlet ,-■-expression water inlet ,-▲-the expression clearance; Fig. 3 is the removal effect figure of sanitary sewage ammonia nitrogen in the embodiment 11, among the figure-◆-expression water outlet ,-■-expression water inlet ,-▲-the expression clearance; Fig. 4 is that cell density and the fat of chlorella in the embodiment 11 contains spirogram, among the figure-◆-expression fat content ,-■-expression cell density; Fig. 5 is the fat productive rate figure during different hydraulic detention time in the embodiment 11.
Embodiment
Embodiment one: undertaken by following step based on the microalgae grease production method of sanitary sewage low-carbon emission resource utilization in the present embodiment: will carry out sterilising treatment through the waste water that one-level is handled, in the lead-in light bio-reactor; In bioreactor, inoculate little algae then, with CO
2Cultivate as the aeration source of the gas, culturing process elder generation intermittent operation to little algae logarithmic growth and density reaches more than the 0.2g/L, changes continuous water inlet then into, control pH remains at 5～10 during the water inlet continuously, collect the little algae in the water outlet, after dewatering, extract grease again, promptly obtain microalgae grease.
Embodiment two: what present embodiment and embodiment one were different is: described waste water is sanitary sewage, beer waste water or molasses containing waste water.Other step is identical with embodiment one with parameter.
Embodiment three: what present embodiment was different with embodiment one or two is: High Temperature High Pressure, Losantin, ozone, Cl are adopted in described sterilization pre-treatment
2, dioxide peroxide or ultraviolet sterilization carry out.Other step is identical with embodiment mode one or two with parameter.
Embodiment four: what present embodiment was different with one of embodiment one to three is: described little algae is chlorella, grid algae, cytoalgae, spirulina or salt algae.Other step is identical with one of embodiment one to three with parameter.
Embodiment five: what present embodiment was different with one of embodiment one to four is: described CO
2Provide by stack gas or air.Other step is identical with one of embodiment one to four with parameter.
Embodiment six: what present embodiment was different with one of embodiment one to five is: described CO
2The speed of aeration is 0.1vvm～2vvm.Other step is identical with one of embodiment one to five with parameter.
Embodiment seven: what present embodiment was different with one of embodiment one to six is: intensity of illumination is 1000lx～20000lx in the described culturing process, light dark period (light:dark) is 8h:16h～24h:0h, and temperature is 20 ℃～40 ℃ in the bioreactor.Other step is identical with one of embodiment one to six with parameter.
Embodiment eight: what present embodiment was different with one of embodiment one to six is: intensity of illumination is 3000lx, and light dark period (light:dark) is 12h:12h, and temperature is 30 ℃ in the bioreactor.Other step is identical with one of embodiment one to six with parameter.
Embodiment nine: what present embodiment was different with one of embodiment one to eight is: the collection method of described little algae adopts coagulation-air supporting method, coagulation-precipitator method, centrifuging or sand filtration method to carry out.Other step is identical with one of embodiment one to eight with parameter.
Embodiment ten: what present embodiment was different with one of embodiment one to nine is: described extraction oil and fat method is chloroform-methanol method, ether-sherwood oil method, Soxhlet extraction process or emp method.Other step is identical with one of embodiment one to nine with parameter.
Embodiment 11: undertaken by following step based on the microalgae grease production method of sanitary sewage low-carbon emission resource utilization in the present embodiment: the sanitary sewage that will handle (handling through grid, precipitation, adjusting pH successively) through one-level adds Losantin and carries out sterilising treatment, in the lead-in light bio-reactor; In bioreactor, inoculate chlorella vulgaris liquid then, the chlorella initial density is 0.05g/L, continuous light, intensity of illumination 3000lx, light dark period (light:dark) is 12h:12h, temperature is 30 ℃ in the bioreactor, cultivate as the aeration source of the gas with air, aeration speed 0.5vvm (airvolume/culture volume/min), culturing process elder generation intermittent operation to little algae logarithmic growth and density reaches 0.2g/L, changes continuous water inlet then into, control pH remains at 8 during the water inlet continuously, by the little algae in coagulation-air supporting method collection water outlet, after the illumination dehydration, adopt the Soxhlet extraction process to extract grease again, promptly obtain microalgae grease.
By Fig. 1～Fig. 3 as can be known, when hydraulic detention time during at 32～96h, 82%～93%, ammonia-N removal rate is 94%～99% to the clearance of COD, and total tp removal rate all reaches the integrated wastewater discharge standard primary standard 89%～98%.Compare with existing sewage disposal technology, present method anti impulsion load ability is stronger, is specially adapted to the changeable area of the discharge of sewage.By Fig. 4, Fig. 5 as can be known, when hydraulic detention time was 96h, cell density was 0.89～0.94g/L, and fat content is 17～20%, and the fat productive rate is 39～48mg/ (Ld); When hydraulic detention time was 48h, cell density was 0.59～0.67g/L, and fat content is 44%～48%, and the fat productive rate is the highest, can reach 156mg/ (Ld); When hydraulic detention time was 32h, cell density was 0.25～0.30g/L, and fat content is 35%～38%.The fat productive rate is 72～86mg/ (Ld).
Claims (10)
1. based on the microalgae grease production method of sanitary sewage low-carbon emission resource utilization, it is characterized in that being undertaken by following step: will carry out sterilising treatment through the waste water that one-level is handled, in the lead-in light bio-reactor based on the microalgae grease production method of sanitary sewage low-carbon emission resource utilization; In bioreactor, inoculate little algae then, with CO
2Cultivate as the aeration source of the gas, culturing process elder generation intermittent operation to little algae logarithmic growth and density reaches more than the 0.2g/L, change continuous water inlet then into, control pH remains at 5～10 during the water inlet continuously, collect the little algae in the water outlet, with extracting grease behind the dewatering microalgae, promptly obtain microalgae grease again.
2. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 1 is characterized in that described waste water is sanitary sewage, beer waste water or molasses containing waste water.
3. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 2 is characterized in that described sterilization pre-treatment employing High Temperature High Pressure, Losantin, ozone, Cl
2, dioxide peroxide or ultraviolet sterilization carry out.
4. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 3 is characterized in that described little algae is chlorella, grid algae, cytoalgae, spirulina or salt algae.
5. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 4 is characterized in that described CO
2Provide by stack gas or air.
6. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 5 is characterized in that described CO
2The speed of aeration is 0.1vvm～2vvm.
7. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 6, it is characterized in that intensity of illumination is 1000lx～20000lx in the described culturing process, light dark period is 8h:16h～24h:0h, and temperature is 20 ℃～40 ℃ in the bioreactor.
8. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 6 is characterized in that intensity of illumination is 3000lx, and light dark period is 12h:12h, and temperature is 30 ℃ in the bioreactor
9. the microalgae grease production method based on sanitary sewage low-carbon emission resource utilization according to claim 7 is characterized in that the collection of described little algae adopts coagulation-air supporting method, coagulation-precipitator method, centrifuging or sand filtration method to carry out.
10. according to the described microalgae grease production method of any claim among the claim 1-9, it is characterized in that described extraction oil and fat method is chloroform-methanol method, ether-sherwood oil method, Soxhlet extraction process or emp method based on sanitary sewage low-carbon emission resource utilization.
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392052A (en) * | 2011-09-22 | 2012-03-28 | 复旦大学 | Biogas purification method by culturing autotrophic freshwater microalgae with biogas slurry |
| CN102618446A (en) * | 2012-04-16 | 2012-08-01 | 北京昊业怡生科技有限公司 | Method for cultivating oleaginous microalgae by using fecal sewage |
| CN102815839A (en) * | 2012-08-27 | 2012-12-12 | 哈尔滨工业大学 | Method and device for low-carbon emission energy regeneration by coupling waste gas and waste water |
| CN102863115A (en) * | 2011-07-07 | 2013-01-09 | 江南大学 | Method for treating fermentation industry waste water and producing algae powder by using microalgae |
| CN103589644A (en) * | 2013-11-28 | 2014-02-19 | 黑龙江省能源环境研究院 | Method for culturing oil-producing microalgae by using brewery sewage |
| CN103922548A (en) * | 2014-05-04 | 2014-07-16 | 北京航空航天大学 | Biological reaction device for culturing high-oil microalgae by using wastewater |
| CN104561153A (en) * | 2015-01-22 | 2015-04-29 | 贵州华清科维环境能源有限责任公司 | Method for producing bio-oil by cultivating algae recycling vinasse nutrients |
| WO2015062157A1 (en) * | 2013-10-29 | 2015-05-07 | 中石化石油工程设计有限公司 | Method for utilizing microalgae in processing oilfield sewage and in co2 fixation |
| CN105802853A (en) * | 2016-05-20 | 2016-07-27 | 佛山市聚成生化技术研发有限公司 | Method for promoting porphyridium growth by virtue of aquatic product processing wastewater |
| CN106867953A (en) * | 2017-03-15 | 2017-06-20 | 哈尔滨工业大学 | A kind of method that microalgae processes molasses containing waste water synchronization production capacity under cryogenic |
| CN108531401A (en) * | 2018-05-30 | 2018-09-14 | 昆明理工大学 | A method of utilizing the useless mash of microalgae processing molasses |
| CN108753622A (en) * | 2018-05-30 | 2018-11-06 | 昆明理工大学 | A method of based on molasses give up mash metal ion improve single needle algae fat content |
| CN108911154A (en) * | 2018-07-16 | 2018-11-30 | 东北师范大学 | A kind of biological treatment waste water containing boron and the method for improving microalgae grease yield |
| CN109534539A (en) * | 2018-11-27 | 2019-03-29 | 东南大学 | A kind of materialization group technology based on the processing of chlorella Ageing solution cycling and reutilization |
| CN110029066A (en) * | 2019-03-14 | 2019-07-19 | 北京联合大学 | A method of utilizing beer waste water culture chlorella |
| CN111635073A (en) * | 2020-06-02 | 2020-09-08 | 江苏海洋大学 | Method for efficiently purifying laver processing wastewater based on economic microalgae culture |
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| CN102863115A (en) * | 2011-07-07 | 2013-01-09 | 江南大学 | Method for treating fermentation industry waste water and producing algae powder by using microalgae |
| CN102863115B (en) * | 2011-07-07 | 2014-07-02 | 江南大学 | Method for treating fermentation industry waste water and producing algae powder by using microalgae |
| CN102392052A (en) * | 2011-09-22 | 2012-03-28 | 复旦大学 | Biogas purification method by culturing autotrophic freshwater microalgae with biogas slurry |
| CN102618446A (en) * | 2012-04-16 | 2012-08-01 | 北京昊业怡生科技有限公司 | Method for cultivating oleaginous microalgae by using fecal sewage |
| CN102815839A (en) * | 2012-08-27 | 2012-12-12 | 哈尔滨工业大学 | Method and device for low-carbon emission energy regeneration by coupling waste gas and waste water |
| WO2015062157A1 (en) * | 2013-10-29 | 2015-05-07 | 中石化石油工程设计有限公司 | Method for utilizing microalgae in processing oilfield sewage and in co2 fixation |
| CN103589644A (en) * | 2013-11-28 | 2014-02-19 | 黑龙江省能源环境研究院 | Method for culturing oil-producing microalgae by using brewery sewage |
| CN103922548A (en) * | 2014-05-04 | 2014-07-16 | 北京航空航天大学 | Biological reaction device for culturing high-oil microalgae by using wastewater |
| CN104561153A (en) * | 2015-01-22 | 2015-04-29 | 贵州华清科维环境能源有限责任公司 | Method for producing bio-oil by cultivating algae recycling vinasse nutrients |
| CN104561153B (en) * | 2015-01-22 | 2019-07-19 | 贵州华清科维环境能源有限责任公司 | A method of it recycling vinasse nutrient culture algae and carries out bio-oil production |
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| CN106867953A (en) * | 2017-03-15 | 2017-06-20 | 哈尔滨工业大学 | A kind of method that microalgae processes molasses containing waste water synchronization production capacity under cryogenic |
| CN108531401A (en) * | 2018-05-30 | 2018-09-14 | 昆明理工大学 | A method of utilizing the useless mash of microalgae processing molasses |
| CN108753622A (en) * | 2018-05-30 | 2018-11-06 | 昆明理工大学 | A method of based on molasses give up mash metal ion improve single needle algae fat content |
| CN108911154A (en) * | 2018-07-16 | 2018-11-30 | 东北师范大学 | A kind of biological treatment waste water containing boron and the method for improving microalgae grease yield |
| CN108911154B (en) * | 2018-07-16 | 2021-05-11 | 东北师范大学 | Method for biologically treating boron-containing wastewater and improving microalgae grease yield |
| CN109534539A (en) * | 2018-11-27 | 2019-03-29 | 东南大学 | A kind of materialization group technology based on the processing of chlorella Ageing solution cycling and reutilization |
| CN110029066A (en) * | 2019-03-14 | 2019-07-19 | 北京联合大学 | A method of utilizing beer waste water culture chlorella |
| CN111635073A (en) * | 2020-06-02 | 2020-09-08 | 江苏海洋大学 | Method for efficiently purifying laver processing wastewater based on economic microalgae culture |
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Application publication date: 20110126 |