CN105802853A - Method for promoting porphyridium growth by virtue of aquatic product processing wastewater - Google Patents
Method for promoting porphyridium growth by virtue of aquatic product processing wastewater Download PDFInfo
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- CN105802853A CN105802853A CN201610341359.4A CN201610341359A CN105802853A CN 105802853 A CN105802853 A CN 105802853A CN 201610341359 A CN201610341359 A CN 201610341359A CN 105802853 A CN105802853 A CN 105802853A
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- aquatic product
- wastewater
- product process
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- porphyridium
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- 238000000034 method Methods 0.000 title claims abstract description 76
- 239000002351 wastewater Substances 0.000 title claims abstract description 40
- 238000012545 processing Methods 0.000 title abstract description 8
- 241000206618 Porphyridium Species 0.000 title abstract description 6
- 230000001737 promoting effect Effects 0.000 title abstract description 4
- 239000000047 product Substances 0.000 claims abstract description 40
- 241000588986 Alcaligenes Species 0.000 claims abstract description 11
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 241001494715 Porphyridium purpureum Species 0.000 claims description 36
- 238000001556 precipitation Methods 0.000 claims description 10
- 241000194035 Lactococcus lactis Species 0.000 claims description 9
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 230000005855 radiation Effects 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 2
- 241000194036 Lactococcus Species 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000004904 shortening Methods 0.000 abstract 1
- 241000195493 Cryptophyta Species 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001020 rhythmical effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
- C02F1/32—Treatment of water, waste water, or sewage by irradiation with ultraviolet light
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
Abstract
The invention discloses a method for promoting porphyridium growth by virtue of aquatic product processing wastewater. The method for promoting porphyridium growth by virtue of the aquatic product processing wastewater comprises the following steps: precipitating the aquatic product processing wastewater; removing precipitate; filtering a supernatant which flows in a circulating mode and applying ultraviolet radiation; fermenting the processed supernatant with the addition of a microbial fermenting agent and adding porphyridium seeds; and breeding, and playing music during breeding, wherein the microbial fermenting agent includes lactococcus and alcaligenes. Compared with the prior art, the method disclosed by the invention has the beneficial effects that the method, which makes full use of the aquatic product processing wastewater, can reduce environmental pollution; and the method is low in cost, short in cycle, capable of shortening a porphyridium growth cycle and achieving large-scale breeding, and the method can bring about huge economic benefits.
Description
Technical field
The invention belongs to microalgae and cultivate field, be specifically related to a kind of method utilizing wastewater from aquatic product process to accelerate Porphyridium cruentum growth.
Background technology
Aquatic products processing wastewater is mainly the waste water that processing of aquatic products process Raw thaws and matting produces.Recently as people's increase to aquatic products demand, a large amount of wastewater from aquatic product process are directly discharged, because in wastewater from aquatic product process, the content of Organic substance and oils and fats and nitrogen phosphorus is all significantly high, direct discharge can increase the weight of the eutrophication of receiving water body, worsen water quality, even causing courses of infection, harm people, animal etc. and fishing activity, so purifying and recycling aquatic products processing wastewater is problem demanding prompt solution.
Porphyridium cruentum (Porhyridium) is the fresh water algae plant of Rhodophyta former Rhodophyceae Porphyridium cruentum order Porphyridium cruentum section Porphyridium, it is distributed in sea water, fresh water, in the soil of salt water and humidity, there is stronger salt-resistance, rich in phycoerythrin, the multiple nutrients material such as polyunsaturated fatty acid and sulfated polysaccharide, exploitation is extracted active substance in Porphyridium cruentum and is always up the focus of research to greatest extent, but owing to the understanding of Porphyridium cruentum optimum growing condition is abundant not, easy pollution microbes in natural environment, and be often cyanophyceae, algae kind of particularly quivering is suppressed, limit the large-scale culture of Porphyridium cruentum.
Summary of the invention
For above-mentioned problems of the prior art, it is an object of the invention to provide a kind of method utilizing wastewater from aquatic product process to accelerate Porphyridium cruentum growth, thoroughly can comprehensively kill the microorganism in Aquatic product waste water, Porphyridium cruentum is made to absorb organic nutrition in Aquatic product waste water to greatest extent, accelerate the growth rate of Porphyridium cruentum, it is achieved its large-scale culture.
For achieving the above object, the technical scheme is that and a kind of method utilizing wastewater from aquatic product process to accelerate Porphyridium cruentum growth is provided, said method comprising the steps of: precipitation wastewater from aquatic product process, go precipitation, supernatant circulates filtration and bestows ultraviolet lighting, putting into Porphyridium cruentum seed after adding microbe leaven fermentation, cultivate, the nurturing period plays music；Described microbe leaven includes lactococcus lactis (Lactococus), Alcaligenes (Alcaligenes).
Preferably, the speed that in said method, supernatant circulates is 0.8-2.6m/s.
Preferably, the number of times that in said method, supernatant circulates is 2-4 time.
Preferably, said method medium ultraviolet intensity of illumination is 15-30mJ/cm2。
Preferably, in said method, in microbe leaven, the adding proportion of lactococcus lactis is 0.3-0.8kg/L, and the adding proportion of Alcaligenes is 0.2-0.6kg/L.
Preferably, in said method, the fermentation time of microbe leaven is 2.5-4d.
Preferably, the music that in said method, the nurturing period plays is " accompanying you to spend the very long years ", " kiss the rain " or " meaning of travelling ".
Preferably, in said method, the nurturing period plays the frequency of music is 1-3 time/d.
Preferably, in said method, the nurturing period plays the time span of music is 30-50min/ time.
Preferably, in said method, cultivation period is 3.5-5.5d.
Alga cells wall is the main network structure being made up of polysaccharide, protein and fat, with certain negative charge, and has bigger surface area and viscosity.Algae can be enriched with and absorb substantial amounts of Organic substance, inorganic matter and heavy metal in growth and breeding process, and amass in richness and organic metabolic degradation occurs simultaneously.
The present invention makes ultraviolet radiation be fully contacted with wastewater from aquatic product process by the mode that wastewater from aquatic product process circulates, and thoroughly kills the miscellaneous bacteria in wastewater from aquatic product process.The organic macromolecule of residual in the microbe leaven lactococcus lactis of interpolation, Alcaligenes energy efficient degradation wastewater from aquatic product process, make and be more easy to the nutrition forms such as the aminoacid, the fatty acid that are absorbed and used, lactococcus lactis can produce a large amount of vitamin B group simultaneously, for promoting that the growth of Porphyridium cruentum provides vitamin endlessly, Alcaligenes can maintain the alkaline pH of wastewater from aquatic product process, and adsorbs the ammonia irritative gas of release in wastewater from aquatic product process sweat.Porphyridium cruentum seed is joined under sufficient nutritional condition and is given its music liked, the speed of growth rapidly increases, because music is a kind of rich rhythmical sound wave, its vibration, Porphyridium cruentum cell being produced a kind of mechanical stimulus, accelerating the metabolism of its cell, thus accelerating growth rate.
Compared with prior art, the invention have the benefit that and make full use of wastewater from aquatic product process, reduce environmental pollution；Cost is low, and the cycle is short, shortens the growth cycle of Porphyridium cruentum, it is achieved its large-scale culture, has huge economic benefit.
Detailed description of the invention
For making those skilled in the art be better understood when technical scheme, below in conjunction with embodiment, technical solution of the present invention is described in detail.
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all commercially obtain.
Embodiment 1
Precipitation wastewater from aquatic product process, goes precipitation, and supernatant is with the speed loop flow filtration 2 times of 0.8m/s and bestows 15mJ/cm2Ultraviolet lighting, adds microbe leaven (China General Microbiological culture presevation administrative center) lactococcus lactis 0.3kg/L, Alcaligenes 0.2kg/L and ferments and put into Porphyridium cruentum seed after 2.5d, cultivate 3.5d, measure its growth rate and OD604nm(spectrophotometer, 723 manually, Shanghai the third woods Electronic Science and Technology Co., Ltd.), result is in Table 1, and the nurturing period plays the music " accompanying you to spend the very long years " of 1 30min.
Embodiment 2
Precipitation wastewater from aquatic product process, goes precipitation, and supernatant is with the speed loop flow filtration 3 times of 1.7m/s and bestows 22.5mJ/cm2Ultraviolet lighting, puts into Porphyridium cruentum seed after adding microbe leaven lactococcus lactis 0.55kg/L, Alcaligenes 0.4kg/L fermentation 3.25d, cultivates 4.5d, measure its growth rate and OD604nm, result is in Table 1, and the nurturing period plays the music " kiss the rain " of 2 40min.
Embodiment 3
Precipitation wastewater from aquatic product process, goes precipitation, and supernatant is with the speed loop flow filtration 4 times of 2.6m/s and bestows 30mJ/cm2Ultraviolet lighting, puts into Porphyridium cruentum seed after adding microbe leaven lactococcus lactis 0.8kg/L, Alcaligenes 0.6kg/L fermentation 4d, cultivates 5.5d, measure its growth rate and OD604nm, result, in Table 1, plays the music " meaning of travelling " of 3 50min daytime nurturing period.
Table 1 Porphyridium cruentum growth rate
| Sample | Biomass |
| Embodiment 1 | OD604nm=1.75 |
| Embodiment 2 | OD604nm=1.80 |
| Embodiment 3 | OD604nm=1.86 |
The Porphyridium cruentum cultivated by the method utilizing wastewater from aquatic product process to accelerate Porphyridium cruentum growth provided by the invention as shown in Table 1 just reaches 1.75 at the 3.5d cell density cultivated, enter the stable phase of growth, it is seen that the method utilizing wastewater from aquatic product process to accelerate Porphyridium cruentum growth provided by the invention can be greatly shortened the growth cycle of Porphyridium cruentum.
Obviously, above-mentioned 4 embodiments are only the section Example of the present invention, rather than whole embodiment.Based on the enlightenment of the present invention, other embodiments all that those of ordinary skill in the art are obtained under the premise not making creative work, broadly fall into the scope that present aspect is protected.
Claims (10)
1. one kind utilizes the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth, it is characterized in that, said method comprising the steps of: precipitation wastewater from aquatic product process, go precipitation, supernatant circulates filtration and bestows ultraviolet lighting, putting into Porphyridium cruentum seed after adding microbe leaven fermentation, cultivate, the nurturing period plays music；In described method, microbe leaven includes lactococcus lactis, Alcaligenes.
2. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that the speed that in described method, supernatant circulates is 0.8-2.6m/s.
3. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that the number of times that in described method, supernatant circulates is 2-4 time.
4. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that described method medium ultraviolet intensity of illumination is 15-30mJ/cm2。
5. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that in described method, in microbe leaven, the adding proportion of lactococcus lactis is 0.3-0.8kg/L, and the adding proportion of Alcaligenes is 0.2-0.6kg/L.
6. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that in described method, the fermentation time of microbe leaven is 2.5-4d.
7. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that the music that in described method, the nurturing period plays is " accompanying you to spend the very long years ", " kiss the rain " or " meaning of travelling ".
8. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that in described method, the nurturing period plays the frequency of music is 1-3 time/d.
9. utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth according to claim 1, it is characterised in that in described method, the nurturing period plays the time span of music is 30-50min/ time.
10. according to any one of claim 1-9, utilize the method that wastewater from aquatic product process accelerates Porphyridium cruentum growth, it is characterised in that in described method, cultivation cycle is 3.5-5.5d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610341359.4A CN105802853A (en) | 2016-05-20 | 2016-05-20 | Method for promoting porphyridium growth by virtue of aquatic product processing wastewater |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610341359.4A CN105802853A (en) | 2016-05-20 | 2016-05-20 | Method for promoting porphyridium growth by virtue of aquatic product processing wastewater |
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| CN105802853A true CN105802853A (en) | 2016-07-27 |
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| CN201610341359.4A Pending CN105802853A (en) | 2016-05-20 | 2016-05-20 | Method for promoting porphyridium growth by virtue of aquatic product processing wastewater |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110643514A (en) * | 2019-10-14 | 2020-01-03 | 贵州民族大学 | Method for culturing porphyridium by using culture wastewater |
| CN113698462A (en) * | 2021-08-13 | 2021-11-26 | 上海藻翰生物科技有限公司 | Method for producing phycoerythrin and porphyridium polysaccharide by coupling wastewater purification |
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|---|---|---|---|---|
| CN1111481A (en) * | 1995-03-20 | 1995-11-15 | Lg电子株式会社 | Food fermentation control system |
| CN101921811A (en) * | 2010-07-20 | 2010-12-22 | 山东省科学院能源研究所 | Method for culturing microalgae |
| CN101955846A (en) * | 2010-10-15 | 2011-01-26 | 哈尔滨工业大学 | Production method of microalgae grease based on low-carbon emission recycling of domestic sewage |
| CN102421887A (en) * | 2009-05-18 | 2012-04-18 | Mbd能源有限公司 | Method of culturing photosynthetic organisms |
| WO2014160350A1 (en) * | 2013-03-14 | 2014-10-02 | Kuehnle Agrosystems, Inc. | Improved wastewater treatment systems and methods |
| CN104388317A (en) * | 2014-12-03 | 2015-03-04 | 东莞市绿安奇生物工程有限公司 | Production method of organic chlorella enriched in trace elements |
-
2016
- 2016-05-20 CN CN201610341359.4A patent/CN105802853A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1111481A (en) * | 1995-03-20 | 1995-11-15 | Lg电子株式会社 | Food fermentation control system |
| CN102421887A (en) * | 2009-05-18 | 2012-04-18 | Mbd能源有限公司 | Method of culturing photosynthetic organisms |
| CN101921811A (en) * | 2010-07-20 | 2010-12-22 | 山东省科学院能源研究所 | Method for culturing microalgae |
| CN101955846A (en) * | 2010-10-15 | 2011-01-26 | 哈尔滨工业大学 | Production method of microalgae grease based on low-carbon emission recycling of domestic sewage |
| WO2014160350A1 (en) * | 2013-03-14 | 2014-10-02 | Kuehnle Agrosystems, Inc. | Improved wastewater treatment systems and methods |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110643514A (en) * | 2019-10-14 | 2020-01-03 | 贵州民族大学 | Method for culturing porphyridium by using culture wastewater |
| CN113698462A (en) * | 2021-08-13 | 2021-11-26 | 上海藻翰生物科技有限公司 | Method for producing phycoerythrin and porphyridium polysaccharide by coupling wastewater purification |
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