CN104388317A - Production method of organic chlorella enriched in trace elements - Google Patents
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Abstract
The invention discloses a production method of organic chlorella enriched in trace elements. The production method comprises the following steps of preparing a biological anaerobic fermentation culture medium, carrying out anaerobic fermentation on a liquid organic fertilizer, producing the organic chlorella and the like. The production method is suitable for experimental production and large-scale production of the chlorella of a level higher than pilot test level. By virtue of the anaerobic fermentation method, the production of the liquid organic fertilizer of the chlorella is achieved, the enrichment and organization of the trace elements are achieved during the preparation of the chlorella cultivation culture medium, the chlorella is cultivated by virtue of the liquid fertilizer enriched in organized trace elements and thus organic chlorella products enriched in various trace elements can be obtained, since various trace elements contained in the chlorella products are organized, the trace elements are easily absorbed by the human body. The chlorella products are prepared from biomass wastes as source materials and thus the recycling and high-valued utilization of the wastes are achieved and the organization of the production process of the chlorella brings environmental and economic double effects.
Description
Technical field
The present invention relates to the production method of a kind of organic chlorella of rich trace elements.Specifically, be utilize the liquid organic fertilizer of rich trace elements to cultivate the chlorella of being rich in iron, zinc, iodine, chromium, selenium and other trace elements, belong to bioengineering field.
Background technology
The elements such as iron, zinc, copper, manganese, chromium, selenium, molybdenum, cobalt, fluorine only account for gross weight less than 0.1% in human body, and these elements are called as the trace element of human body, and trace element is closely bound up with the health of human body, plays vital effect to the life of people.According to research, up to the present, be identified the essential trace element relevant with HUMAN HEALTH and life and have 18 kinds, namely iron is had, copper, zinc, cobalt, manganese, chromium, selenium, iodine, nickel, fluorine, molybdenum, vanadium, tin, silicon, strontium, boron, rubidium, arsenic etc., although they are minimum at people's in-vivo content, but their materials that to be some the conclusive metabolism maintained in human body indispensable, as the prothetic group that iron is hemachrome enzyme, iodine is Triiodothyronine constituent, copper is the prothetic group of Terminal oxidase, manganese is the cofactor of arginase etc., zinc is deoxygenase, archaeal dna polymerase, the cofactor of carbonic anhydrase, selenium is the cofactor of Selenoperoxidase etc., cobalt is the composition of vitamin B12, vanadium is the cofactor of nitrate reductase, molybdenum is the cofactor of XOD, nickel is the cofactor of urease.Trace element excess intake, deficiency, imbalance or shortage all can cause the exception of Human Physiology to some extent or disease occurs.At present, compare it is clear that the disease of about 30% is directly caused by trace element deficiency or imbalance, study discovery simultaneously, trace element disease-resistant, give protection against cancer, to promote longevity etc. in all also play very important effect.
Trace element is mainly present in Soil and Rock at occurring in nature, exist with the form of inorganics, the trace element of needed by human body needs to be taken in by diet, but in real life, human body is not high to the assimilated efficiency of inorganic form trace element, most people often do not obtain enough trace elements by diet, need additionally to compensate.
Chlorella is a kind of natural food balanced in nutrition, there is effect of multi-function health-care, contain growth in humans's growth, necessary multiple nutrients material of having good health and a long life, in chlorella, protein Protein content is high, and aminoacids content is balanced, meets human nutrition demand, and its protein peptide has multiple biological activity, can activating human body cells be played, repair defective gene, promote the effects such as organ tissue growth; Containing multiple efficiency natural VITAMIN in chlorella, there is anti-oxidant, scavenging free radicals, delay senility, improve the function such as the heart, pulmonary function, chlorella algae polysaccharide and various trace elements, have the effects such as antifatigue, atherosclerosis, preventing cardiovascular disease and diabetes; The CGF active factor that chlorella is exclusive, have immunity moderation function, antitumor, bring out Interferon, rabbit, strengthen the function such as cytophagy; Natural highly enriched chlorophyll and plant cellulose in chlorella, have and get rid of chemical toxicant in body, heavy metal, protection liver, kidney from effects such as murder by poisoning, preventing cancer.
Chlorella is in process of growth, having a significant biological property is can micro-metal ion in enrichment culture liquid, and there is the function inorganic microelement in environment being converted into the trace element organised, more be beneficial to absorption of human body, but in actual production, chlorella cultivates the photoautotrophic mode of many employings, inorganic microelement is applied directly in substratum, be difficult to control the microelement concentration in frustule, and have considerable part to be just adsorbed on cell surface, be still inorganic states, be not easily absorbed by the body; In addition, still may there is a large amount of trace elements remaining in the water body of substratum, discharge easily causes environmental pollution.
The invention provides the production method of a kind of organic chlorella of rich trace elements, utilize chlorella can carry out the biological characteristics of Heterotrophic culture with organism, the substratum being enriched the trace element that organises is produced by the method for microbial cells fermentation, this substratum cultivates for chlorella after allocating further, can produce the chlorella obtaining and be rich in various trace element.
Summary of the invention
The object of the invention is to the production method of the chlorella that a kind of rich trace elements is provided for the deficiencies in the prior art, the method may be used for cultivating, producing the chlorella of being rich in all kinds of trace element, and the micronutrient levels controlled comparatively accurately in frond, and frond medium trace element organic degree is high, be more conducive to absorption of human body, improve the quality of chlorella product, achieve chlorella product diversification, organise and high-valued further.In addition, the method utilizes industrial or agricultural abandoned biomass as main raw material, achieves changing waste into resources, environmental benefit and social benefit obvious
For achieving the above object, the present invention is achieved through the following technical solutions.
A production method for organic chlorella of rich trace elements, includes following processing step, is specially:
A. microbiological anaerobic fermention medium preparation: utilize abandoned biomass for main raw material, prepare burden according to its nutrition composition, supplements the macroelement such as nitrogen, phosphorus, potassium according to demand and regulates substratum various element balance, add zinc, chromium (Cr simultaneously
3+), the inorganic salt of the element such as selenium, iodine prepare burden as trace element; Pass into steam sterilizing after raw material pulverizing, mixing, add sterilized water and be deployed into anaerobically fermenting substratum;
B. liquid organic fertilizer anaerobically fermenting: is added cellulose-decomposing bacterium and proteolysis bacterium bacterial classification in the substratum that step a is prepared, under anaerobic ferment, obtain containing organic fermented liquids such as monose, oligosaccharides, low first organic acid, total free aminoacids, protein peptide, thalline, in fermented liquid, be rich in zinc, chromium (Cr simultaneously
3+), the trace element such as selenium, iodine;
C. organic chlorella produces: by the fermented liquid sterilizing of the rich trace elements that step b obtains, after allotment, be added into chlorella Cultivation container, carry out chlorella cultivation; After chlorella has cultivated, centrifugally to gather, dry, namely obtain the organic chlorella product being enriched all kinds of trace element, can develop for follow-up further processing.
Wherein, abandoned biomass described in step a, the waste that all kinds of industrial and agricultural productions referred to produce and by product, the biomass resource of fermentable can be supplied, as fermentation waste water, brewing wastewater, vinasse, manioc waste, bagasse, dregs of beans, peanut meal, rice bran, wheat bran, agricultural stalk etc. containing protein, polysaccharide (being mainly starch and Mierocrystalline cellulose) etc.
Wherein, the nutrition composition described in step a, mainly refers to the material such as protein and polysaccharide that can be utilized by microorganism in abandoned biomass; Allotment described in step a, refer to according to the component content such as albumen, polysaccharide in different waste biomass materials, proportioning raw material by a certain percentage, and the supplementary of correspondence adds the fertilizer such as nitrogen, phosphorus, potassium, reaches and is suitable for nutrition needed for fermentable growth and element proportioning; The interpolation of the trace element described in step a, refers to the needs according to target chlorella product, and corresponding adds zinc, chromium (Cr
3+), the inorganic salt of the trace element such as selenium, iodine.
Wherein, liquid organic fertilizer anaerobically fermenting described in step b, under referring to oxygen free condition, the process of cellulose-decomposing bacterium, amylolysis bacterium, steatolysis bacterium and proteolysis bacterium is cultivated in the culture medium culturing of step a preparation, its principle utilizes cellulose-decomposing bacterium, and in the process of amylolysis bacterium and steatolysis bacterium growth and breeding in the medium, the material such as Mierocrystalline cellulose, starch, fat decomposed in substratum becomes the monose, oligosaccharides, low first organic acid etc. that other mushrooms or chlorella can directly utilize; Utilize proteolysis bacterium, in the process of growth and breeding in the medium, decomposition of protein class material, as the material such as thalline, albumen be chlorella can directly utilize total free aminoacids, protein small peptide process.
Further, cellulose-decomposing bacterium described in step b, is referred to and can under anaerobic, be produced and metabolism by thalline, Mierocrystalline cellulose in decomposing biomass is the bacterial classification of low first carbohydrate such as monose, oligosaccharides and formic acid, acetic acid, propionic acid, butyric acid, as each kind of strain of Clostridium.
Further, amylolysis bacterium described in step b, is referred to and can under anaerobic, be produced and metabolism by thalline, starch in decomposing biomass is the bacterial classification of low first carbohydrate such as monose, oligosaccharides and formic acid, acetic acid, propionic acid, butyric acid, as some of Bacillus subtilus plant strain.
Further, steatolysis bacterium described in step b, is referred to and can under anaerobic, be produced and metabolism by thalline, fat in decomposing biomass is the bacterial classification of low first carbohydrate such as monose, oligosaccharides and formic acid, acetic acid, propionic acid, butyric acid, as Clostridium and Bacillus subtilus.
Further, protein decomposing bacteria described in step b, is referred to and can under anaerobic, be produced and metabolism by thalline, protein in decomposing biomass is the bacterial classification of total free aminoacids and protein small peptide, and in environment, most anerobe all has protelytic function.
Wherein, chlorella cultivation described in step c, refer to utilize step b to obtain the fermented liquid being rich in monose, oligosaccharides, low first organic acid, total free aminoacids, protein small peptide and each trace element as substratum, as chlorella cultivate raw material, in culture vessel, cultivate the process of chlorella.Further, what chlorella culture vessel referred to must be fermentor tank, Closed photobioreactor and open pond, and the series combination of above different vessels.
Wherein, centrifugal described in step c is gathered, and refers to the process utilizing the centrifugal chlorella of gathering of disc centrifuge to obtain chlorella underflow.
Wherein, the drying described in step c, refers to and utilizes spray-drying tower dried pellet algae underflow, obtains the process of chlorella dry powder.
beneficial effect of the present invention is:organic chlorella production method of a kind of rich trace elements of the present invention, it comprises the preparation of microbiological anaerobic fermention medium, the processing steps such as liquid organic fertilizer anaerobically fermenting and the production of organic chlorella.By above processing step, produce the organic chlorella product obtaining and be rich in various trace element.The present invention is applicable to experimental production and the production of mass-producing chlorella of more than pilot scale rank; The present invention is by the method for anaerobically fermenting, achieve the production of the liquid organic fertilizer of chlorella, namely achieve the enrichment of trace element in the link of chlorella cultivation substratum configuration and organise, utilize this to be rich in organise the liquid fertilizer cultivation chlorella of trace element, the organic chlorella product being enriched each trace element can be obtained, each trace element in this chlorella product organises, and is more easily absorbed by the body.The present invention utilizes biomass waste for source material, and achieve changing rejected material to useful resource and high-valued, organising of chlorella production process, brings environment and economy double effect.
Embodiment
Below in conjunction with concrete embodiment, the present invention will be described, wherein, the chlorella of indication of the present invention is each chlorella vulgaris strain being defined as Chlorella (Chlorella) in taxonomy, and equipment of the present invention all refers to pilot scale and the above scale equipment of pilot scale.
Embodiment one, utilize brewer's grains for main raw material configuration microbiological anaerobic fermention medium, Dongguan Jin Wei brew-house taken from by brewer's grains, by analysis, containing crude protein 24.7%, crude fat 7.5%, robust fibre 14.2% in dry-matter; Get water content be 45% brewer's grains 700 kilograms join in 3 tons of stainless steel sterilization tanks, add 50 kilograms, molasses, add water 750 kilograms and stirring and evenly mixings, logical steam, and 120 DEG C of heat sterilizations more than 0.5 hour, after being down to room temperature, proceed in 5 tons of plastic tanks after sterilization.
Get 20 kilograms, urea, salt 15 kilograms, potassium primary phosphate 10 kilograms, magnesium sulfate heptahydrate 15 kilograms, 2 kilograms, iron(ic) chloride, Zinc Sulphate Heptahydrate 3 kilograms, Sodium Selenite 0.75 gram, Potassium Iodate 1 gram, chromium nitrate [Cr (NO
3)
3] 0.75 gram, after fully melting filtration sterilization respectively, add in 5 tons of plastic tanks, adding sterilized water to cumulative volume is 3.5 tons, has namely configured microbiological anaerobic fermention medium.
Embodiment two, chlorella liquid organic fertilizer is produced, in the microbiological anaerobic fermention medium that embodiment one obtains, be in 5 tons of plastic barrels of 3.5 tons at liquid amount, by the addition of substratum 10g per ton, add commercially available EM bacterium powder, this bacterium powder is containing bacterial classifications such as bifidocaterium, milk-acid bacteria, genus bacillus, photosynthetic bacterium, yeast, actinomycetes, acetic bacterias, and every gram containing total count >=50,000,000,000 CFU.After plastic tank seals, be positioned over outdoor, each anerobe in EM bacterium powder utilizes natural temperature condition to grow, and the anaerobically fermenting time is more than 7 days.Get fermented supernatant fluid, after 0.5 hour, namely obtain concentrated liquid organic fertilizer through 120 DEG C of high-temperature sterilizations, be wherein rich in the trace elements such as iron, zinc, selenium, trivalent chromium and iodine.Because anaerobic fermentation conditions is uncontrolled, in the fertilizer that different batches is produced, each the concentrating of nutrients is indefinite, need measure nitrogen element content for reference index, as the foundation of the organic substratum of configuration chlorella after lower step dilution.
Embodiment three, chlorella heterotrophy fermentation culture, the present embodiment is with 10 tonnes of fermentor tanks for culture vessel, and adding aseptic heterotrophic fermentation chlorella liquid is seed liquor and aseptic concentrated liquid fertilizer, adds sterilized water, to the reaction volume of 7 tons.Control in starting fermentation liquid, chlorella dry weight density is 0.25g ± 0.05/L, in substratum, nitrogen element final concentration is 0.22 ~ 0.25gN/L, the parameter of fermentation culture: stir speed (S.S.) 180 ~ 200rpm, it is 50 ± 5% that dissolved oxygen controls, leavening temperature is 28 ± 0.5 DEG C, it is 7 ± 0.2 that pH controls, ferment after 24 hours, results algae liquid, utilize disc centrifuge centrifugal concentrating, centrifugation rate is more than 4500rpm, again after repeatedly cleaning-centrifugal 3 times, spray-dried for concentrated solution (dry weight content is about 10%) tower is dry, drying temperature controls at 180 DEG C, namely organic chlorella algae powder of fermentative production is obtained after drying.After testing, in fermented liquid, chlorella dry weight content is 0.82g/L, protein content 48.7% in the chlorella of this organic production, moisture <5%, ash content <7%, Zn content is 5.2mg/g, trivalent chromium content is 22 μ g/g, iron level is 2.5mg/g, and selenium content is 19 μ g/g, and iodine content is 31 μ g/g.
Embodiment four, Closed photobioreactor is utilized to cultivate chlorella, the present embodiment with sterilization after vertical pocket type bioreactor for culture vessel, the diameter of vertical bag is 25cm, height is 2.2 meters, and working fluid is high is 1.75 meters, and working volume is 85L, 12 vertical bags are one group, and total working volume is 1 cubic metre.Add chlorella seed liquor and aseptic concentrated liquid fertilizer, supplement sterilized water, control in initial inoculum, chlorella dry weight density is 0.15g ± 0.05/L, in substratum, nitrogen element final concentration is 0.12 ~ 0.15gN/L, air agitation, inflation is than being 0.05vvm, under natural light, be filled with carbon dioxide with supplementary carbon source, and control pH is 8 ± 0.2, respectively adding nitrogen element final concentration in once concentration liquid organic fertilizer to nutrient solution in every morning and afternoon is ~ 0.15gN/L, at outside scenery, natural light is utilized to produce.Measure between incubation period, water temperature is 22 ~ 31 DEG C, maximum intensity of illumination is 55 ~ 65klux, cultivates after three days, results algae liquid, utilize disc centrifuge centrifugal concentrating, centrifugation rate is more than 4500rpm, then after repeatedly cleaning-centrifugal 3 times, spray-dried for concentrated solution (dry weight content is about 10%) tower is dry, drying temperature controls at 180 DEG C, namely obtains organic chlorella algae powder after drying.After testing, in fermented liquid, chlorella dry weight content is 1.25g/L, protein content 61.2% in the chlorella of this organic production, moisture <5%, ash content <7%, Zn content is 6.4 mg/g, trivalent chromium content is 26 μ g/g, iron level is 3.2 mg/g, and selenium content is 23 μ g/g, and iodine content is 37 μ g/g.
Embodiment five, fermentor tank and the series connection of vertical bag reactor is utilized to carry out chlorella cultivation, the present embodiment with 10 tonnes of fermentor tanks for culture vessel, culturing step and parameter are with embodiment three, ferment after 24 hours, proceed to in vertical pocket type bioreactor, culturing step and parameter are with embodiment four, cultivate (inoculation at 18 in afternoon after 24 hours, through 1 sunshine), results algae liquid, utilize disc centrifuge centrifugal concentrating, centrifugation rate is more than 4500rpm, again after repeatedly cleaning-centrifugal 3 times, spray-dried for concentrated solution (dry weight content is about 10%) tower is dry, drying temperature controls at 180 DEG C, namely organic chlorella algae powder is obtained after drying.After testing, in fermented liquid, chlorella dry weight content is 1.02g/L, protein content >60.2% in the chlorella of this organic production, moisture <5%, ash content <7%, Zn content is 5.7 mg/g, trivalent chromium content is 23 μ g/g, iron level is 2.9mg/g, and selenium content is 21 μ g/g, and iodine content is 34 μ g/g.
Embodiment six, chlorella cultivation is carried out in open pond, with 1000 square metres of circular ponds of opening for culture vessel, add the water of the 10cm degree of depth, add chlorella seed concentrated liquor body fertilizer, make up water is 20cm to the cultivation degree of depth, control in initial inoculum, chlorella dry weight density is 0.12g ± 0.02/L, in substratum, nitrogen element final concentration is, utilize transport mixer to stir, rotating speed is that every 72s mono-encloses, and it is 0.12 ~ 0.15gN/L that every morning adds nitrogen element final concentration in once concentration fertilizer to substratum.At outside scenery, natural light is utilized to produce.Measure between incubation period, water temperature is 24 ~ 32 DEG C, maximum intensity of illumination is 58 ~ 67klux, cultivates after four days, results algae liquid, utilize disc centrifuge centrifugal concentrating, centrifugation rate is more than 4500rpm, then after repeatedly cleaning-centrifugal 3 times, spray-dried for concentrated solution (dry weight content is about 10%) tower is dry, drying temperature controls at 180 DEG C, namely obtains organic chlorella algae powder after drying.After testing, in fermented liquid, chlorella dry weight content is 0.58g/L, protein content 59.7% in the chlorella of this organic production, moisture <5%, ash content <7%, Zn content is 6.4 mg/g, trivalent chromium content is 32 μ g/g, iron level is 3.5mg/g, and selenium content is 27 μ g/g, and iodine content is 42 μ g/g.
Above content is only preferred embodiment of the present invention, for those of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, as added the proportioning of each raw material in micro-kind and batching, the variation of parameters in fermentation culture, the application concentration etc. of fertilizer in breeding process, this description should not be construed as limitation of the present invention.
Claims (4)
1. an organic chlorella production method for rich trace elements, is characterized in that: it comprises the following steps, and the first step is the preparation of microbiological anaerobic fermention medium; Second step is producing of fertilizer anaerobic fermented liquid; 3rd step is in culture vessel, using the cultivation raw material of fertilizer anaerobic fermented liquid as chlorella, adds chlorella seed liquor and carries out chlorella cultivation, carries out organic chlorella product that drying can obtain rich trace elements after results algae liquid.
2. organic chlorella production method of a kind of rich trace elements according to claim 1, it is characterized in that: the preparation of microbiological anaerobic fermention medium, utilize abandoned biomass for main raw material, prepare burden according to its nutrition composition, supplement the fertilizer such as nitrogen, phosphorus, potassium and regulate substratum various element balance, add zinc, chromium (Cr simultaneously
3+), the inorganic salt element such as selenium, iodine prepares burden as trace element; Pass into steam sterilizing after being pulverized by above-mentioned compound, add sterilized water and be deployed into anaerobically fermenting substratum; Described abandoned biomass, refer to the waste that all kinds of industrial and agricultural production produces and by product, the biomass of fermentable can be supplied, as fermentation waste water, brewing wastewater, vinasse, manioc waste, bagasse, dregs of beans, peanut meal, rice bran, wheat bran, agricultural stalk etc. containing protein, polysaccharide (being mainly starch and Mierocrystalline cellulose) etc.; Described nutrition composition, refers to the material such as protein and polysaccharide that can be utilized by microorganism in abandoned biomass; Described allotment, refer to according to the component content such as albumen, polysaccharide in different waste biomass materials, by a certain percentage proportioning raw material, and the supplementary of correspondence adds the fertilizer such as nitrogen, phosphorus, potassium, reach be suitable for fermentable growth needed for nutrition and element proportioning; The interpolation of described trace element, refers to the needs according to target chlorella product, and corresponding adds zinc, chromium (Cr
3+), the inorganic salt element of the trace such as selenium, iodine.
3. organic chlorella production method of a kind of rich trace elements according to claim 1, it is characterized in that: producing of fertilizer anaerobic fermented liquid, cellulose-decomposing bacterium and proteolysis bacterium bacterial classification will be added in microbiological anaerobic fermention medium, under anaerobic ferment, obtain containing organic fermented liquids such as monose, oligosaccharides, low first organic acid, total free aminoacids, protein peptide, thalline, in fermented liquid, be rich in zinc, chromium (Cr simultaneously
3+), the trace element such as selenium, iodine; Described fertilizer anaerobically fermenting, under referring to oxygen free condition, the process of cellulose-decomposing bacterium, amylolysis bacterium, steatolysis bacterium and proteolysis bacterium is cultivated in microbiological anaerobic fermention medium, its principle utilizes cellulose-decomposing bacterium, and in the process of amylolysis bacterium and steatolysis bacterium growth and breeding in the medium, the material such as Mierocrystalline cellulose, starch, fat decomposed in substratum becomes monose, oligosaccharides and the low first organic acid etc. that other mushrooms or chlorella can directly utilize; Utilize proteolysis bacterium, in the process of growth and breeding in the medium, decomposition of protein class material, if the material such as thalline, albumen is the process of the chlorella total free aminoacids that can directly utilize and protein small peptide;
Further, described cellulose-decomposing bacterium, is referred to and can under anaerobic, be produced and metabolism by thalline, Mierocrystalline cellulose in decomposing biomass is the bacterial classification of low first carbohydrate such as monose, oligosaccharides and formic acid, acetic acid, propionic acid, butyric acid, as each kind of strain of Clostridium;
Further, described amylolysis bacterium, is referred to and can under anaerobic, be produced and metabolism by thalline, starch in decomposing biomass is the bacterial classification of low first carbohydrate such as monose, oligosaccharides and formic acid, acetic acid, propionic acid, butyric acid, as some of Bacillus subtilus plant strain;
Further, described steatolysis bacterium, is referred to and can under anaerobic, be produced and metabolism by thalline, fat in decomposing biomass is the bacterial classification of low first carbohydrate such as monose, oligosaccharides and formic acid, acetic acid, propionic acid, butyric acid, as Clostridium and Bacillus subtilus;
Further, described protein decomposing bacteria, refer to and can under anaerobic, be produced and metabolism by thalline, the protein in decomposing biomass is the bacterial classification of total free aminoacids and protein small peptide, and in environment, most anerobe all has protelytic function.
4. organic chlorella production method of a kind of rich trace elements according to claim 1, it is characterized in that: organic chlorella produces, by the fermented liquid sterilizing of the rich trace elements of acquisition, after allotment, be added into chlorella Cultivation container, carry out chlorella cultivation; After chlorella has cultivated, centrifugally to gather, dry, namely obtain the organic chlorella product being enriched all kinds of trace element, can develop for follow-up further processing; Described chlorella cultivation, refer to and utilize the fermented liquid being rich in monose, oligosaccharides, low first organic acid, total free aminoacids, protein small peptide and each trace element obtained as substratum, as the raw material that chlorella is cultivated, in culture vessel, cultivate the process of chlorella; Further, what chlorella culture vessel referred to must be fermentor tank, Closed photobioreactor or open pond, and the series combination of above different vessels; Described centrifugally to gather, refer to the process utilizing the centrifugal chlorella of gathering of disc centrifuge to obtain chlorella underflow; Described drying, refers to and utilizes spray-drying tower dried pellet algae underflow, obtains the process of chlorella dry powder.
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| CN108410920A (en) * | 2018-05-10 | 2018-08-17 | 天津大学 | The optimization method of polysaccharide and grease is produced using high concentration tofu wastewater culture chlorella L166 |
| CN109897784A (en) * | 2019-02-27 | 2019-06-18 | 天津大学 | A kind of method that novel two stages autotrophy-Heterotrophic culture promotes microalgae lipid |
| CN110194567A (en) * | 2019-06-19 | 2019-09-03 | 北京林业大学 | A kind of processing method of sludge hydrolysis, acidification liquid |
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| CN105802853A (en) * | 2016-05-20 | 2016-07-27 | 佛山市聚成生化技术研发有限公司 | Method for promoting porphyridium growth by virtue of aquatic product processing wastewater |
| CN106701588A (en) * | 2016-11-17 | 2017-05-24 | 中盐工程技术研究院有限公司 | Culture method of selenium-enriched chlorella |
| CN108410920A (en) * | 2018-05-10 | 2018-08-17 | 天津大学 | The optimization method of polysaccharide and grease is produced using high concentration tofu wastewater culture chlorella L166 |
| CN109897784A (en) * | 2019-02-27 | 2019-06-18 | 天津大学 | A kind of method that novel two stages autotrophy-Heterotrophic culture promotes microalgae lipid |
| CN110194567A (en) * | 2019-06-19 | 2019-09-03 | 北京林业大学 | A kind of processing method of sludge hydrolysis, acidification liquid |
| CN111320510A (en) * | 2020-02-28 | 2020-06-23 | 广州市土根旺生物科技有限公司 | Directional fermentation microbial fertilizer and preparation method thereof |
| CN112430161A (en) * | 2020-12-01 | 2021-03-02 | 鹤山市新的生物制品有限公司 | Organic fermented paste fertilizer and preparation method thereof |
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