CN104388350A - Microbial agent capable of improving quality of water for aquaculture and method for preparing same - Google Patents
Microbial agent capable of improving quality of water for aquaculture and method for preparing same Download PDFInfo
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- CN104388350A CN104388350A CN201410675914.8A CN201410675914A CN104388350A CN 104388350 A CN104388350 A CN 104388350A CN 201410675914 A CN201410675914 A CN 201410675914A CN 104388350 A CN104388350 A CN 104388350A
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- actinomycetes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a microbial agent capable of improving the quality of water for aquaculture. The microbial agent is prepared from the following effective components in percentage by mass: 20-30% of bacillus subtilis, 10-20% of bacillus megaterium, 10-20% of photosynthetic bacteria, 5-15% of actinomycetes, 2-10% of saccharomycetes, 10-25% of lactobacillus and 8-20% of streptococcus faecalis. The invention further discloses a method for preparing the microbial agent. The method comprises the following steps: inoculating a solid culturing medium against the bacillus subtilis, the bacillus megaterium, the photosynthetic bacteria, the actinomycetes, the saccharomycetes, the lactobacillus and the streptococcus faecalis for activation; then getting each single colony on a panel by an inoculating loop, inoculating each liquid culturing medium against the corresponding single colony, and culturing to obtain liquid seed microbial agents; and inoculating each fermentation tank against the corresponding liquid seed microbial agent for fermentation, filtering, concentrating and collecting microbes, mixing the microbes in a required proportion, and adding a nutrient solution selectively to obtain the composite microbial agent.
Description
Technical field
The present invention relates to a kind of microbiobacterial agent can improveing aquaculture system water quality, and the preparation method of this microbial inoculum.
Background technology
Under the certain environmental conditions such as illumination, temperature, in water body, the state of supply of solvability nutritive ingredient just directly determines speed and the output of water body primary productoin, and finally affects cultured fishes output.
Traditional aquaculture model that it is technical characteristic that existing pond aquaculture has acted on " close, mixed, wheel " mostly, namely with multi items, high-density, heavy dressing bait throwing in and obtain the aquaculture model of high yield, the materials such as ammonia nitrogen in water, phosphorus, nitrite, hydrogen sulfide are caused to surge, transparency declines, water quality deterioration, residual bait in water body, drug residue, larger molecular organics matter etc. accumulate in pond bottom in a large number, and substrate pollution is serious.
In water body and substrate, excessive organic matter decomposition not only consumes the dissolved oxygen in water body, water body environment is made to be in anoxic condition, also discharge COD, TSS and many harmful levels of pathogens simultaneously, grow a large amount of and harmful algae, endanger the aquaculture organism in water body directly or indirectly, species diversity in aquaculture water is declined, and the cultivation of high-quality aquatic products kind is subject to the restriction of water quality and food organism.
Water body natural, ecological is destroyed, and in pond, the disease of aquatic animal occurs frequent, and fishery loss is huge.The control of ecological environment of cultivating pool has become the key issue of development China pond aquaculture.
Summary of the invention
Main purpose of the present invention is to provide a kind of dissolved oxygen amount increasing water body, suppresses the microbiobacterial agent that harmful levels of pathogens and harmful algae grow.
The technical solution adopted for the present invention to solve the technical problems is: a kind of microbiobacterial agent improveing aquaculture system water quality, and the effective constituent following by mass percent forms:
Bacillus subtillis 20-30%
Bacillus megaterium 10-20%
Photosynthetic bacterium 10-20%
Actinomycetes 5-15%
Yeast 2-10%
Milk-acid bacteria 10-25%
Streptococcus faecium 8-20%.
Described a kind of microbiobacterial agent improveing aquaculture system water quality, the effective constituent following by mass percent forms: Bacillus subtillis 25%, bacillus megaterium 15%, photosynthetic bacterium 15%, actinomycetes 12%, yeast 8%, milk-acid bacteria 15%, streptococcus faecium 10%.
Described a kind of microbiobacterial agent improveing aquaculture system water quality, the viable count in every milliliters of liquid complex micro organism fungicide is 2-8 hundred million.
Another object of the present invention is to provide a kind of preparation method improveing the microbiobacterial agent of aquaculture system water quality.
The technical solution adopted for the present invention to solve the technical problems is: a kind of preparation method improveing the microbiobacterial agent of aquaculture system water quality, comprises the following steps: a), by Bacillus subtillis, bacillus megaterium, photosynthetic bacterium, actinomycetes, yeast, milk-acid bacteria and streptococcus faecium be inoculated in respectively on solid medium and activate; B), with single bacterium colony that transfering loop is made even on plate be inoculated in respectively in corresponding liquid nutrient medium and cultivate, obtain liquid bacterial agent seed liquor; C), by above-mentioned seed liquor access in fermentor tank respectively and ferment, filtering and concentrating collects thalline, and according to the mixing of required ratio, selectivity adds nutritive medium, obtains complex micro organism fungicide.
The invention has the beneficial effects as follows: microbiobacterial agent strong adaptability of the present invention, dominant population can be formed by Fast-propagation after applying water body, residual bait in fast decoupled aquaculture water, drug residue, larger molecular organics matter etc., the materials such as degradation of ammonia nitrogen, phosphorus, nitrite, hydrogen sulfide, potent removal COD, TSS, increase the dissolved oxygen amount of water body, suppress growing of harmful levels of pathogens and harmful algae, for hydrocoles creates the good environment of an activation, promote the gastrointestinal peristalsis of hydrocoles simultaneously, increase food consumption, improve the utilization ratio of feed.
Embodiment
Embodiment one
The microbiobacterial agent of the present embodiment, the effective constituent following by mass percent forms: Bacillus subtillis 20%, bacillus megaterium 20%, photosynthetic bacterium 20%, actinomycetes 8%, yeast 2%, milk-acid bacteria 10% and streptococcus faecium 20%, the viable count in every milliliters of liquid complex micro organism fungicide is 2-8 hundred million.
The present invention is the essential product realizing ecological fishery.
Preparation method comprises the following steps: a), by Bacillus subtillis, bacillus megaterium, photosynthetic bacterium, actinomycetes, yeast, milk-acid bacteria and streptococcus faecium be inoculated in respectively on solid medium and activate; B), with single bacterium colony that transfering loop is made even on plate be inoculated in respectively in corresponding liquid nutrient medium and cultivate, obtain liquid bacterial agent seed liquor; C), by above-mentioned seed liquor access in fermentor tank respectively and ferment, filtering and concentrating collects thalline, and according to the mixing of required ratio, selectivity adds nutritive medium, obtains complex micro organism fungicide.
Embodiment two
The microbiobacterial agent of the present embodiment, the effective constituent following by mass percent forms: Bacillus subtillis 30%, bacillus megaterium 10%, photosynthetic bacterium 10%, actinomycetes 15%, yeast 8%, milk-acid bacteria 19% and streptococcus faecium 8%.
Preparation method is identical with embodiment one.
Embodiment three
The microbiobacterial agent of the present embodiment, the effective constituent following by mass percent forms: Bacillus subtillis 25%, bacillus megaterium 15%, photosynthetic bacterium 15%, actinomycetes 12%, yeast 8%, milk-acid bacteria 15%, streptococcus faecium 10%.
Preparation method is the same.
Embodiment four
The microbiobacterial agent of the present embodiment, the effective constituent following by mass percent forms: Bacillus subtillis 22%, bacillus megaterium 15%, photosynthetic bacterium 15%, actinomycetes 5%, yeast 10%, milk-acid bacteria 25%, streptococcus faecium 8%.
Preparation method is the same.
Aquatic products special fertilizer dissolution rate of the present invention is fast, utilization ratio is high, the bait of aquatic animal can be impelled to breed, beneficial algae can be trained for a certain area again, the nutrition of comprehensive and abundant can be provided, suppress the generation of fish disease to fish, can water quality be improved simultaneously, increase the dissolved oxygen in water, plant plankton can be supplied to and grow required nutritive element fast, promote that algae is to the absorption of inorganic nutrients and chlorophyllous synthesis, improves the stability of pond ecosystem, make the water quality condition that pond keeps best.
Above-described embodiment is illustrative principle of the present invention and effect thereof only; and the embodiment that part is used, for the person of ordinary skill of the art, without departing from the concept of the premise of the invention; can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (4)
1. improve a microbiobacterial agent for aquaculture system water quality, it is characterized in that, the effective constituent following by mass percent forms:
Bacillus subtillis 20-30%
Bacillus megaterium 10-20%
Photosynthetic bacterium 10-20%
Actinomycetes 5-15%
Yeast 2-10%
Milk-acid bacteria 10-25%
Streptococcus faecium 8-20%.
2. a kind of microbiobacterial agent improveing aquaculture system water quality according to claim 1, it is characterized in that, the effective constituent following by mass percent forms: Bacillus subtillis 25%, bacillus megaterium 15%, photosynthetic bacterium 15%, actinomycetes 12%, yeast 8%, milk-acid bacteria 15%, streptococcus faecium 10%.
3. a kind of microbiobacterial agent improveing aquaculture system water quality according to claim 1 and 2, is characterized in that, the viable count in every milliliters of liquid complex micro organism fungicide is 2-8 hundred million.
4. improve a preparation method for the microbiobacterial agent of aquaculture system water quality as claimed in claim 1, it is characterized in that, comprise the following steps:
A), Bacillus subtillis, bacillus megaterium, photosynthetic bacterium, actinomycetes, yeast, milk-acid bacteria and streptococcus faecium are inoculated on solid medium respectively activate;
B), with single bacterium colony that transfering loop is made even on plate be inoculated in respectively in corresponding liquid nutrient medium and cultivate, obtain liquid bacterial agent seed liquor;
C), by above-mentioned seed liquor access in fermentor tank respectively and ferment, filtering and concentrating collects thalline, and according to the mixing of required ratio, selectivity adds nutritive medium, obtains complex micro organism fungicide.
Priority Applications (1)
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| CN201410675914.8A CN104388350A (en) | 2014-11-24 | 2014-11-24 | Microbial agent capable of improving quality of water for aquaculture and method for preparing same |
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| CN201410675914.8A CN104388350A (en) | 2014-11-24 | 2014-11-24 | Microbial agent capable of improving quality of water for aquaculture and method for preparing same |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105595010A (en) * | 2015-09-01 | 2016-05-25 | 嘉兴佳康丽生物科技有限公司 | Water quality balance ferment for aquaculture and organic sewage treatment and preparation method thereof |
| CN106242075A (en) * | 2016-08-22 | 2016-12-21 | 怀远县渔业科技发展有限责任公司 | A kind of loach breeding water improvement preparation and preparation method thereof |
| CN107285484A (en) * | 2017-08-21 | 2017-10-24 | 苏州神良生物科技有限公司 | A kind of freshwater aquiculture microorganism formulation |
| CN107324504A (en) * | 2017-08-09 | 2017-11-07 | 中国科学院水生生物研究所 | A kind of compound algae microbial inoculum improved for culture-pool water quality and preparation method thereof |
| CN107487853A (en) * | 2017-08-16 | 2017-12-19 | 雷州市恒业水产有限公司 | One kind is used for aquaculture water transfer microorganism formulation |
| CN107535393A (en) * | 2017-09-08 | 2018-01-05 | 无为县慧富水蛭养殖专业合作社 | A kind of degraded dispensing of hirudiniculture |
| CN107628668A (en) * | 2017-11-08 | 2018-01-26 | 钟华 | Mixed bacteria, strain carrier and the method for sustainable degraded creek black and odorous water petrochina pollutant |
| CN107628669A (en) * | 2017-11-08 | 2018-01-26 | 钟华 | Mixed bacteria, strain carrier and the method for volatile phenol pollutant in sustainable degraded creek black and odorous water |
| CN107673563A (en) * | 2017-11-08 | 2018-02-09 | 钟华 | Mixed bacteria, strain carrier and the method for sulfide in sustainable degraded creek black and odorous water |
| CN107698038A (en) * | 2017-11-08 | 2018-02-16 | 钟华 | Mixed bacteria, strain carrier and the method for heavy metal in sustainable degraded creek black and odorous water |
| CN109607819A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of control of microorganisms aquaculture method |
| CN109601446A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of prawn culturing |
| CN110002611A (en) * | 2019-04-24 | 2019-07-12 | 无锡正和岛生物科技有限公司 | A kind of breeding water body regulator and preparation method thereof |
| CN110304737A (en) * | 2019-07-09 | 2019-10-08 | 常德市万惠生物科技有限公司 | A kind of compound probiotic and preparation method thereof |
| CN113057264A (en) * | 2021-03-08 | 2021-07-02 | 嘉达生物科技(广州)有限责任公司 | Directly used aquatic probiotic powder and preparation method thereof |
| CN113801835A (en) * | 2021-09-27 | 2021-12-17 | 广东省科学院动物研究所 | Process for preparing algae culture nutrient solution by using hermetia illucens sand |
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105595010A (en) * | 2015-09-01 | 2016-05-25 | 嘉兴佳康丽生物科技有限公司 | Water quality balance ferment for aquaculture and organic sewage treatment and preparation method thereof |
| CN106242075A (en) * | 2016-08-22 | 2016-12-21 | 怀远县渔业科技发展有限责任公司 | A kind of loach breeding water improvement preparation and preparation method thereof |
| CN107324504A (en) * | 2017-08-09 | 2017-11-07 | 中国科学院水生生物研究所 | A kind of compound algae microbial inoculum improved for culture-pool water quality and preparation method thereof |
| CN107324504B (en) * | 2017-08-09 | 2019-10-08 | 中国科学院水生生物研究所 | A kind of compound algae microbial inoculum and preparation method thereof for culture-pool water quality improvement |
| CN107487853A (en) * | 2017-08-16 | 2017-12-19 | 雷州市恒业水产有限公司 | One kind is used for aquaculture water transfer microorganism formulation |
| CN107285484A (en) * | 2017-08-21 | 2017-10-24 | 苏州神良生物科技有限公司 | A kind of freshwater aquiculture microorganism formulation |
| CN107535393A (en) * | 2017-09-08 | 2018-01-05 | 无为县慧富水蛭养殖专业合作社 | A kind of degraded dispensing of hirudiniculture |
| CN107673563A (en) * | 2017-11-08 | 2018-02-09 | 钟华 | Mixed bacteria, strain carrier and the method for sulfide in sustainable degraded creek black and odorous water |
| CN107628669A (en) * | 2017-11-08 | 2018-01-26 | 钟华 | Mixed bacteria, strain carrier and the method for volatile phenol pollutant in sustainable degraded creek black and odorous water |
| CN107698038A (en) * | 2017-11-08 | 2018-02-16 | 钟华 | Mixed bacteria, strain carrier and the method for heavy metal in sustainable degraded creek black and odorous water |
| CN107628668A (en) * | 2017-11-08 | 2018-01-26 | 钟华 | Mixed bacteria, strain carrier and the method for sustainable degraded creek black and odorous water petrochina pollutant |
| CN109601446A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of prawn culturing |
| CN109601446B (en) * | 2018-11-23 | 2021-11-05 | 广东海纳川生物科技股份有限公司 | Preparation and application of special microbial preparation for stable water of prawn culture |
| CN109607819A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of control of microorganisms aquaculture method |
| CN110002611A (en) * | 2019-04-24 | 2019-07-12 | 无锡正和岛生物科技有限公司 | A kind of breeding water body regulator and preparation method thereof |
| CN110304737A (en) * | 2019-07-09 | 2019-10-08 | 常德市万惠生物科技有限公司 | A kind of compound probiotic and preparation method thereof |
| CN113057264A (en) * | 2021-03-08 | 2021-07-02 | 嘉达生物科技(广州)有限责任公司 | Directly used aquatic probiotic powder and preparation method thereof |
| CN113801835A (en) * | 2021-09-27 | 2021-12-17 | 广东省科学院动物研究所 | Process for preparing algae culture nutrient solution by using hermetia illucens sand |
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Application publication date: 20150304 |