CN107324504A - A kind of compound algae microbial inoculum improved for culture-pool water quality and preparation method thereof - Google Patents

A kind of compound algae microbial inoculum improved for culture-pool water quality and preparation method thereof Download PDF

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CN107324504A
CN107324504A CN201710673708.7A CN201710673708A CN107324504A CN 107324504 A CN107324504 A CN 107324504A CN 201710673708 A CN201710673708 A CN 201710673708A CN 107324504 A CN107324504 A CN 107324504A
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algae
parts
solution
individual
zymocyte liquid
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CN107324504B (en
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王启烁
张善夫
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Institute of Hydrobiology of CAS
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00Specific treatment goals
    • C02F2303/04Disinfection

Abstract

The invention discloses a kind of compound algae microbial inoculum improved for culture-pool water quality, its raw material is made up of the component of following parts by weight:15~25 parts of chlorella pyrenoidosa algae solution, 30~50 parts of goat's horn crescent moon algae algae solution, 15~25 parts of Scenedesmus quadricauda algae solution, 5~15 parts of Fermentation by Photosynthetic Bacteria bacterium solution, 10~20 parts of Sphaerotilus natans zymocyte liquid, 10~20 parts of thiobacillus denitrificans zymocyte liquid, 5~15 parts of bacillus natto to ferment bacterium solution, 5~15 parts of saccharomycetes to make fermentation bacterium solution, 1~2 part of sodium humate.In addition, present invention also offers a kind of preparation method for the compound algae microbial inoculum improved for culture-pool water quality.The compound algae microbial inoculum of the present invention is applied after water body, utilize the metabolic activity of microorganism, organic pollutants are shifted, converted and degraded, reduce water pollutant concentration, and energy Synergistic, the harmful substance such as ammonia nitrogen, nitrous acid, hydrogen sulfide in decomposition water, improves cultivating pool water body environment.

Description

A kind of compound algae microbial inoculum improved for culture-pool water quality and preparation method thereof
Technical field
The invention belongs to aquiculture waste water processing technology field, and in particular to a kind of to be used for what culture-pool water quality was improved Compound algae microbial inoculum and preparation method thereof.
Background technology
With developing rapidly for China's aquaculture, proportion of the aquaculture in aquaculture is continued to increase, due to market The continuous expansion of demand, the cultivation density of aquaculture is also continued to increase;With the lasting increasing of cultivation density, the water quality brought Deterioration can not be ignored, and be mainly shown as:The water pollution that fish faeces are caused, and human lives' sewage, rural sewage Influence, cause water quality deterioration.Ammonia nitrogen, nitrite, dissolved oxygen amount in some breeding water body water quality serious agings, water body etc. are supported Grow parameter continuous worsening;Beneficial algae active level is greatly reduced, and the situation of aging and stagnant water is increasingly serious, and photosynthesis subtracts It is few, show that the pH value within one day does not change, or change atomic, it is not suitable for the life growth of fish.
Just because of the continuous worsening of aquatic environment, fish disease takes place frequently, and aquaculture family largely uses medical treatment, particularly The abuse degree increase of antibiotic, so as to more accelerate the deterioration of water quality situation, and has a negative impact to fish meat, enters And fishery -ies product outlet is affected;The generation of germ drug resistance, the preventing and treating to fish disease brings bigger difficulty.
Algae is as the important primary producer of aquatic ecosystem, purifying water, during aquatic ecological restoration by more Carry out the attention of more researchers, the A of Chinese patent CN 105254019 disclose the formula that a kind of aquaculture handles waste water, its In used chlorella pyrenoidosa, three kinds of algae of scenedesmus obliquus and crescent moon algae aquiculture waste water handled, the processing side Although method can effectively control the nuisance content such as ammoniacal nitrogen in breeding water body, nitrite nitrogen to a certain extent, improve water quality, But a large amount of algae are needed during processing waste water, and algae easily breeds, so as to cause wawter bloom phenomenon occur;In addition, algae is certainly Also the frustule of substantial amounts of organic substance and death can be secreted during body growth and breeding, this partial contamination thing also can be to water Body brings secondary pollution.
The content of the invention
The invention provides a kind of compound algae microbial inoculum improved for culture-pool water quality, prior art Chinese medicine is solved Thing preventing and treating aquatic environment, which deteriorates, causes the abuse degree of antibiotic to increase, so as to more accelerate the deterioration of water quality situation, also solves The problem of simple use algae pollution administration water body of having determined easily brings secondary pollution.
First purpose of the present invention is to provide a kind of compound algae microbial inoculum improved for culture-pool water quality, its raw material It is made up of the component of following parts by weight:15~25 parts of chlorella pyrenoidosa algae solution, 30~50 parts of goat's horn crescent moon algae algae solution, four tails 15~25 parts of grid algae algae solution, 5~15 parts of Fermentation by Photosynthetic Bacteria bacterium solution, 10~20 parts of Sphaerotilus natans zymocyte liquid, denitrogenation sulphur bar 10~20 parts of bacterium zymocyte liquid, 5~15 parts of bacillus natto to ferment bacterium solution, 5~15 parts of saccharomycetes to make fermentation bacterium solution, sodium humate 1~2 part;
Wherein, the concentration of the chlorella pyrenoidosa algae solution is 1.0 × 105~2.0 × 105Individual/mL;
The concentration of the goat's horn crescent moon algae algae solution is 1.0 × 105~3.0 × 105Individual/mL;
The concentration of the Scenedesmus quadricauda algae solution is 1.0 × 105~2.0 × 105Individual/mL;
Living bacteria count in living bacteria count, the Sphaerotilus natans zymocyte liquid in the Fermentation by Photosynthetic Bacteria bacterium solution, It is living bacteria count in living bacteria count, the bacillus natto to ferment bacterium solution in the thiobacillus denitrificans zymocyte liquid, described In saccharomycetes to make fermentation bacterium solution living bacteria count >=109Individual/mL.
It is preferred that, it is described for culture-pool water quality improve compound algae microbial inoculum raw material by following parts by weight group It is grouped into:20 parts of chlorella pyrenoidosa algae solution, 40 parts of goat's horn crescent moon algae algae solution, 20 parts of Scenedesmus quadricauda algae solution, Fermentation by Photosynthetic Bacteria 10 parts of bacterium solution, 15 parts of Sphaerotilus natans zymocyte liquid, 15 parts of thiobacillus denitrificans zymocyte liquid, bacillus natto to ferment bacterium solution 10 Part, 10 parts of saccharomycetes to make fermentation bacterium solution, 1 part of sodium humate zymocyte liquid;
Wherein, the concentration of the chlorella pyrenoidosa algae solution is 2.0 × 105Individual/mL;
The concentration of the goat's horn crescent moon algae algae solution is 2.0 × 105Individual/mL;
The concentration of the Scenedesmus quadricauda algae solution is 1.0 × 105Individual/mL;
Living bacteria count is 4.5 × 10 in the Fermentation by Photosynthetic Bacteria bacterium solution9Individual/mL, the Sphaerotilus natans zymophyte Living bacteria count is 1.0 × 10 in liquid10Living bacteria count is 3.0 × 10 in individual/mL, the thiobacillus denitrificans zymocyte liquid9Individual/ Living bacteria count is 3.5 × 10 in mL, bafillus natto work zymocyte liquid9In individual/mL, the saccharomycetes to make fermentation bacterium solution Living bacteria count is 2.5 × 109Individual/mL.
Second object of the present invention is to provide a kind of preparation for the compound algae microbial inoculum improved for culture-pool water quality Method, comprises the following steps:
Step 1, it is 1.0 × 10 chlorella pyrenoidosa culture to be tamed to concentration5~2.0 × 105Individual/mL, obtains the egg White nucleus chlorella algae solution;
It is 1.0 × 10 by goat's horn crescent moon algae culture domestication to concentration5~3.0 × 105Individual/mL, obtains the goat's horn crescent moon algae Algae solution;
It is 1.0 × 10 that Scenedesmus quadricauda culture, which is tamed to concentration,5~2.0 × 105Individual/mL, obtains the Scenedesmus quadricauda algae Liquid;
Step 2, photosynthetic bacteria, Sphaerotilus natans, thiobacillus denitrificans, bafillus natto, saccharomycete are fermented training respectively Support, obtain respective zymocyte liquid;And living bacteria count, the Sphaerotilus natans are sent out in the Fermentation by Photosynthetic Bacteria bacterium solution Living bacteria count, the bacillus natto to ferment bacterium in living bacteria count, the thiobacillus denitrificans zymocyte liquid in yeast-like fungi liquid In liquid in living bacteria count, the saccharomycetes to make fermentation bacterium solution living bacteria count >=109Individual/mL;
Step 3,15~25 parts of the chlorella pyrenoidosa algae solution, 30~50 parts of the goat's horn crescent moon algae algae solution, institute are weighed State 15~25 parts of Scenedesmus quadricauda algae solution, 5~15 parts of the Fermentation by Photosynthetic Bacteria bacterium solution, the Sphaerotilus natans zymocyte liquid 10~ 20 parts, 10~20 parts of the thiobacillus denitrificans zymocyte liquid, 5~15 parts of the bacillus natto to ferment bacterium solution, the yeast 5~15 parts of bacterium zymocyte liquid, 1~2 part of sodium humate, above-mentioned raw materials are mixed and stirred for uniformly, that is, to obtain the compound algae Microbial inoculum.
Compared with prior art, the beneficial effects of the present invention are:
1) present invention carries out microalgae, profitable strain and sodium humate scientific and reasonable integration, obtains compound algae bacterium Agent, the compound algae microbial inoculum is applied after water body, using bacteria metabolism activity, organic pollutants are shifted, Conversion and degrade, reduce water pollutant concentration, and can Synergistic, decompose Ammonia Nitrogen in Aquatic Environment, nitrous acid, hydrogen sulfide etc. Harmful substance, improves culture pond water body environment.
2) in the present invention microalgae by inorganic nutrient salts such as absorbed nitrogen, phosphorus synthesis of organic substance, and can be to around discharging Oxygen, improves Dissolved Oxygen in Water content;On the one hand profitable strain plays a part of Synergistic to the removal of pollutant in water body, On the other hand the frustule using the organic substance secreted by algae and death can also be decomposed, its catabolite can enter one again Step is absorbed by algae, the two food source each other, so as to avoid the generation of secondary pollution.
Embodiment
In order that those skilled in the art more fully understand that technical scheme can be practiced, with reference to specific The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Microalgae and bacterial strain used are bought in Chinese agriculture Microbiological Culture Collection administrative center in the present invention, following each Experimental method described in embodiment, is conventional method unless otherwise specified.
Chlorella pyrenoidosa algae solution, goat's horn crescent moon algae algae solution, Scenedesmus quadricauda algae solution are to use conventional method in embodiment Expand culture, domestication and obtain algae solution;Fermentation by Photosynthetic Bacteria bacterium solution, Sphaerotilus natans zymocyte liquid, thiobacillus denitrificans zymocyte liquid, Bacillus natto to ferment bacterium solution, saccharomycetes to make fermentation bacterium solution are to expand culture using conventional method to obtain bacterium solution.
Embodiment 1
It is a kind of for culture-pool water quality improve compound algae microbial inoculum, its raw material by following parts by weight component group Into:15 parts of chlorella pyrenoidosa algae solution, 30 parts of goat's horn crescent moon algae algae solution, 25 parts of Scenedesmus quadricauda algae solution, Fermentation by Photosynthetic Bacteria bacterium solution 5 Part, 20 parts of Sphaerotilus natans zymocyte liquid, 10 parts of thiobacillus denitrificans zymocyte liquid, 15 parts of bacillus natto to ferment bacterium solution, ferment Female 5 parts of bacterium zymocyte liquid, 2 parts of sodium humate;
Wherein, the concentration of chlorella pyrenoidosa algae solution is 1.0 × 105Individual/mL;
The concentration of goat's horn crescent moon algae algae solution is 2.0 × 105Individual/mL;
The concentration of Scenedesmus quadricauda algae solution is 1.5 × 105Individual/mL;
Living bacteria count is 1.0 × 10 in Fermentation by Photosynthetic Bacteria bacterium solution10In individual/mL, Sphaerotilus natans zymocyte liquid effectively Viable count is 3.0 × 109Living bacteria count is 2.0 × 10 in individual/mL, thiobacillus denitrificans zymocyte liquid9Individual/mL, natto gemma bar Living bacteria count is 3.5 × 10 in bacterium work zymocyte liquid9Living bacteria count is 5.5 × 10 in individual/mL, saccharomycetes to make fermentation bacterium solution9 Individual/mL;
Specific implementation step is as follows:
Step 1, it is 1.0 × 10 that chlorella pyrenoidosa is cultivated to domestication to concentration according to a conventional method5Individual/mL, obtains albumen Core chlorella algae solution;
It is 2.0 × 10 that goat's horn crescent moon algae is cultivated into domestication to concentration according to a conventional method5Individual/mL, obtains goat's horn crescent moon algae algae Liquid;
It is 1.5 × 10 that Scenedesmus quadricauda is cultivated to domestication to concentration according to a conventional method5Individual/mL, obtains Scenedesmus quadricauda algae solution;
Step 2, by photosynthetic bacteria, Sphaerotilus natans, thiobacillus denitrificans, bafillus natto, saccharomycete difference routinely Method fermented and cultured, obtains respective zymocyte liquid;Living bacteria count is 1.0 × 10 in Fermentation by Photosynthetic Bacteria bacterium solution10Individual/ Living bacteria count is 3.0 × 10 in mL, Sphaerotilus natans zymocyte liquid9Effective viable bacteria in individual/mL, thiobacillus denitrificans zymocyte liquid Number is 2.0 × 109Living bacteria count is 3.5 × 10 in individual/mL, bafillus natto work zymocyte liquid9Individual/mL, saccharomycetes to make fermentation Living bacteria count is 5.5 × 10 in bacterium solution9Individual/mL;
Step 3,15 parts of chlorella pyrenoidosa algae solution, 30 parts of goat's horn crescent moon algae algae solution, 25 parts of Scenedesmus quadricauda algae solution, light are weighed Close 5 parts of bacterial fermentation bacterium solution, 20 parts of Sphaerotilus natans zymocyte liquid, 10 parts of thiobacillus denitrificans zymocyte liquid, bafillus natto 15 parts of zymocyte liquid, 5 parts of saccharomycetes to make fermentation bacterium solution, 2 parts of sodium humate, above-mentioned raw materials are mixed and stirred for uniformly, that is, to be answered Close algae microbial inoculum.
Embodiment 2
It is a kind of for culture-pool water quality improve compound algae microbial inoculum, its raw material by following parts by weight component group Into:20 parts of chlorella pyrenoidosa algae solution, 40 parts of goat's horn crescent moon algae algae solution, 20 parts of Scenedesmus quadricauda algae solution, Fermentation by Photosynthetic Bacteria bacterium solution 10 parts, 15 parts of Sphaerotilus natans zymocyte liquid, 15 parts of thiobacillus denitrificans zymocyte liquid, 10 parts of bacillus natto to ferment bacterium solution, 10 parts of saccharomycetes to make fermentation bacterium solution, 1 part of sodium humate;
Wherein, the concentration of chlorella pyrenoidosa algae solution is 2.0 × 105Individual/mL;
The concentration of goat's horn crescent moon algae algae solution is 2.0 × 105Individual/mL;
The concentration of Scenedesmus quadricauda algae solution is 1.0 × 105Individual/mL;
Living bacteria count is 4.5 × 10 in Fermentation by Photosynthetic Bacteria bacterium solution9In individual/mL, Sphaerotilus natans zymocyte liquid effectively Viable count is 1.0 × 1010Living bacteria count is 3.0 × 10 in individual/mL, thiobacillus denitrificans zymocyte liquid9Individual/mL, natto gemma Living bacteria count is 3.5 × 10 in bacillus work zymocyte liquid9Living bacteria count is 2.5 × 10 in individual/mL, saccharomycetes to make fermentation bacterium solution9 Individual/mL;
Specific implementation step is as follows:
Step 1, it is 2.0 × 10 that chlorella pyrenoidosa is cultivated to domestication to concentration according to a conventional method5Individual/mL, obtains albumen Core chlorella algae solution;
It is 2.0 × 10 that goat's horn crescent moon algae is cultivated into domestication to concentration according to a conventional method5Individual/mL, obtains goat's horn crescent moon algae algae Liquid;
It is 1.0 × 10 that Scenedesmus quadricauda is cultivated to domestication to concentration according to a conventional method5Individual/mL, obtains Scenedesmus quadricauda algae solution;
Step 2, by photosynthetic bacteria, Sphaerotilus natans, thiobacillus denitrificans, bafillus natto, saccharomycete difference routinely Method fermented and cultured, obtains respective zymocyte liquid;Living bacteria count is 4.5 × 10 in Fermentation by Photosynthetic Bacteria bacterium solution9Individual/ Living bacteria count is 1.0 × 10 in mL, Sphaerotilus natans zymocyte liquid10Effective viable bacteria in individual/mL, thiobacillus denitrificans zymocyte liquid Number is 3.0 × 109Living bacteria count is 3.5 × 10 in individual/mL, bafillus natto work zymocyte liquid9Individual/mL, saccharomycetes to make fermentation Living bacteria count is 2.5 × 10 in bacterium solution9Individual/mL;
Step 3,20 parts of chlorella pyrenoidosa algae solution, 40 parts of goat's horn crescent moon algae algae solution, 20 parts of Scenedesmus quadricauda algae solution, light are weighed Close 10 parts of bacterial fermentation bacterium solution, 15 parts of Sphaerotilus natans zymocyte liquid, 15 parts of thiobacillus denitrificans zymocyte liquid, bafillus natto 10 parts of zymocyte liquid, 10 parts of saccharomycetes to make fermentation bacterium solution, 1 part of sodium humate, above-mentioned raw materials are mixed and stirred for uniformly, that is, to be answered Close algae microbial inoculum.
Embodiment 3
It is a kind of for culture-pool water quality improve compound algae microbial inoculum, its raw material by following parts by weight component group Into:25 parts of chlorella pyrenoidosa algae solution, 50 parts of goat's horn crescent moon algae algae solution, 15 parts of Scenedesmus quadricauda algae solution, Fermentation by Photosynthetic Bacteria bacterium solution 15 parts, 10 parts of Sphaerotilus natans zymocyte liquid, 20 parts of thiobacillus denitrificans zymocyte liquid, 5 parts of bacillus natto to ferment bacterium solution, ferment Female 15 parts of bacterium zymocyte liquid, 1 part of sodium humate;
Wherein, the concentration of chlorella pyrenoidosa algae solution is 1.5 × 105Individual/mL;
The concentration of goat's horn crescent moon algae algae solution is 3.0 × 105Individual/mL;
The concentration of Scenedesmus quadricauda algae solution is 1.0 × 105Individual/mL;
Living bacteria count is 4.0 × 10 in Fermentation by Photosynthetic Bacteria bacterium solution9In individual/mL, Sphaerotilus natans zymocyte liquid effectively Viable count is 5.0 × 109Living bacteria count is 3.0 × 10 in individual/mL, thiobacillus denitrificans zymocyte liquid9Individual/mL, natto gemma bar Living bacteria count is 1.5 × 10 in bacterium work zymocyte liquid10Living bacteria count is 3.5 × 10 in individual/mL, saccharomycetes to make fermentation bacterium solution9 Individual/mL;
Specific implementation step is as follows:
Step 1, it is 1.5 × 10 that chlorella pyrenoidosa is cultivated to domestication to concentration according to a conventional method5Individual/mL, obtains albumen Core chlorella algae solution;
It is 3.0 × 10 that goat's horn crescent moon algae is cultivated into domestication to concentration according to a conventional method5Individual/mL, obtains goat's horn crescent moon algae algae Liquid;
It is 1.0 × 10 that Scenedesmus quadricauda is cultivated to domestication to concentration according to a conventional method5Individual/mL, obtains Scenedesmus quadricauda algae solution;
Step 2, by photosynthetic bacteria, Sphaerotilus natans, thiobacillus denitrificans, bafillus natto, saccharomycete difference routinely Method fermented and cultured, obtains respective zymocyte liquid;Living bacteria count is 4.0 × 10 in Fermentation by Photosynthetic Bacteria bacterium solution9Individual/ Living bacteria count is 5.0 × 10 in mL, Sphaerotilus natans zymocyte liquid9Effective viable bacteria in individual/mL, thiobacillus denitrificans zymocyte liquid Number is 3.0 × 109Living bacteria count is 1.5 × 10 in individual/mL, bafillus natto work zymocyte liquid10Individual/mL, saccharomycete hair Living bacteria count is 3.5 × 10 in yeast-like fungi liquid9Individual/mL;
Step 3,25 parts of chlorella pyrenoidosa algae solution, 50 parts of goat's horn crescent moon algae algae solution, 15 parts of Scenedesmus quadricauda algae solution, light are weighed Close 15 parts of bacterial fermentation bacterium solution, 10 parts of Sphaerotilus natans zymocyte liquid, 20 parts of thiobacillus denitrificans zymocyte liquid, bafillus natto 5 parts of zymocyte liquid, 15 parts of saccharomycetes to make fermentation bacterium solution, 1 part of sodium humate, above-mentioned raw materials are mixed and stirred for uniformly, that is, to be answered Close algae microbial inoculum.
Embodiment 1~3 has prepared the compound algae microbial inoculum that culture-pool water quality is improved that is used for worked well, below It is described further with reference to the application of compound algae microbial inoculum in practice come the effect to the present invention.
Application case 1
1st, fish pond is tested:Jiangsu Province Huai'an Xuyi County Tie Fo towns Mao Er lakes, 10 mu of water surface area, 1.8 meters of the depth of water is applied Before, floating head phenomenon often occurs for water body aging, fish, and water quality index refers to table 1.
2nd, breed variety:Hybridized prussian carp " middle section 3 ".
3rd, test method:
The compound algae microbial inoculum prepared using embodiment 1, amount of application is 2kg/ mus, adds 100 times of breeding water body dilutions Equably splash into cat lake afterwards, night on the same day opens aerator and coordinated.
Before the compound algae microbial inoculum prepared using embodiment 1, water sampling detection is used as control sample;Fetched water using after 5 days Sample detection is used as experiment sample.And when sample time is afternoon 14, pH detection times are respectively detected when being at 9 points in the morning and afternoon 14 Once.Specific test result is shown in Table 1.
The embodiment 1 of table 1 is combined algae microbial inoculum application effect
As can be seen from Table 1, the compound algae microbial inoculum prepared using embodiment 1 is after 5 days, and water quality has more significant Change, illustrate that the compound algae microbial inoculum effect that embodiment 1 is prepared is notable.
Application case 2
1st, fish pond is tested:Test the common fish pond that fish pond is Jiangsu Province Huai'an Hongze area Xishunhe town, pond area 8 Mu, pond is deep 1.5~1.8 meters, and bottom of pond enters draining convenient without substantial amounts of mud.
2nd, breed variety:Grass carp.
3rd, test method:
The compound algae microbial inoculum prepared using embodiment 2, amount of application is 1.5kg/ mus, adds 100 times of breeding water bodies dilute Equably splashed after releasing into fish pond, night on the same day opens aerator and coordinated.
Before the compound algae microbial inoculum prepared using embodiment 2, water sampling detection is used as control sample;Fetched water using after 7 days Sample detection is used as experiment sample.And when sample time is afternoon 14, pH detection times are respectively detected when being at 9 points in the morning and afternoon 14 Once.Specific test result is shown in Table 2.
The embodiment 2 of table 2 is combined algae microbial inoculum application effect
As can be seen from Table 2, the compound algae microbial inoculum prepared using embodiment 2 is after 7 days, and water quality has more significant Change, illustrate that the compound algae microbial inoculum effect that embodiment 2 is prepared is notable.
Application case 3
1st, fish pond is tested:Jiangsu Province Huai'an Lianshui County ripples cities and towns, 2 mu of water surface for aquaculture of experiment, 1.5 meters of the depth of water, before, Water body algae is mutually main based on blue-green algae, blue-green algae quantity 3.68 × 107Individual/L, water body aging, fish often raises the nose above water to breathe.
2nd, breed variety:Carp.
3rd, test method
The compound algae microbial inoculum prepared using embodiment 3, amount of application is 1kg/ mus, adds 100 times of breeding water body dilutions Equably splash afterwards into fish pond, night on the same day opens aerator and coordinated.
Fry is added in by the end of March, 2016, apply the compound algae microbial inoculum that embodiment 3 is prepared, 7~August water transfer two by the end of April It is secondary, new water is filled daily, starts the pool to November then, regard the data of culture pond in 2016 as experiment sample;Control group is used The data of 1 year on the culture pond, wherein, the fry amount of control group dispensing, release time, go out pool time, the species of feeding feed It is identical with test group with feeding amount.Specific test result is shown in Table 3.
The fish culture performance table of 3 embodiment of table 3
From table 3 it can be seen that total fish catches substantially increase after the compound algae microbial inoculum prepared using embodiment 3, reach 1785kg/ mus, culture efficiency improves notable, and feed consumption is reduced, and illustrates that the compound algae microbial inoculum that embodiment 3 is prepared is comprehensive Close high efficiency.And applied embodiment 3 and be combined that the fish quality for cultivating out after algae microbial inoculum is good, storage tolerance, the fish sold exists In the market deposits two days, not dead fish, without exception, and epidermal mucus enriches.In addition, feed coefficient decreases, to bait when going out pond Coefficient is expected 1.6 or so, and the feed coefficient of control group is 2.1 or so, direct economic benefit increase by 15% or so, effect is very Significantly.
Addition chlorella pyrenoidosa, goat's horn crescent moon algae and Scenedesmus quadricauda, these three algaes in the compound algae microbial inoculum of the present invention Class synthesis of organic substance by inorganic nutrient salts such as absorbed nitrogen, phosphorus, raising Dissolved Oxygen in Water content, and the raising of dissolved oxygen content It can be suppressed the breeding of the anaerobic bacterias such as vibrios, while reducing the accumulation of reproducibility harmful toxic matter, suppress blue-green algae and dinoflagellate Growth and breeding, be conducive to the growth and breeding of the beneficial algae such as green alga and diatom, stable bacterium phase and algae phase improve the disease-resistant of fish Power.
Contain bacteriochlorin in the photosynthetic bacteria cell that the present invention is used, under anaerobic and illumination condition, using in water body Organic matter and inorganic nitrogen carry out photosynthesis, synthesize a large amount of thalline, can remove ammonia nitrogen in water body, nitrite nitrogen and inorganic The materials such as phosphorus;Sphaerotilus natans can secret out of a variety of high activity ectoenzymes (protease, amylase and hydrolysis of hemicellulose enzyme etc.), These enzymes can decompose and absorb the organic matter in water environment, improve the water body of eutrophication, COD in water body can be removed well Deng pollutant;Amino compound in water body can be converted into the nitrate or nitrous acid of plant available by thiobacillus denitrificans Salt compounds;Bafillus natto in growth course it is metabolizable go out polyglutamic acid, polyglutamic acid is a kind of high molecular polymerization Thing, beneficial bacterial strain can be fixed thereon face, and can be by the materials such as the protein in cultivation water, starch flocculation aggregation, formation Biological flocculation, can also be used as feed while purifying water;Not only fungal cell is big, metabolic capability is strong for saccharomycete, resistance to Thief zone Pressure, resisting high-concentration, and there is oxidation, suction-operated for larger molecular organicses.
The various microalgaes that can form dominant microflora and strain reasonable compatibility are obtained compound algae microbial inoculum by the present invention, and this is answered Closing removal of the algae microbial inoculum to the pollutant in breeding water body, especially ammonia nitrogen has synergistic function, is combined algae microbial inoculum In each microalgae, strain symbiosis coordinate, sewage treating efficiency is higher than single culture and single microalgae;In addition, profitable strain On the one hand the frustule using the organic substance secreted by algae and death can be decomposed, its catabolite again can be further by algae Class is absorbed, the two food source each other, so as to avoid the generation of secondary pollution;On the other hand, due to microorganism and algae There is competitive relation, therefore, it can suppress the quick breeding of algae to a certain extent, it is to avoid the generation of wawter bloom phenomenon.
It should be noted that when being related to number range in claims of the present invention, it is thus understood that each number range Any one numerical value can select between two end points and two end points, due to step method and the phase of embodiment 1~3 of use Together, in order to prevent from repeating, the present invention describes preferred embodiment, but those skilled in the art once know basic wound The property made concept, then can make other change and modification to these embodiments.So, appended claims are intended to be construed to include Preferred embodiment and fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies Within, then the present invention is also intended to comprising including these changes and modification.

Claims (3)

1. a kind of compound algae microbial inoculum improved for culture-pool water quality, it is characterised in that its raw material is by following parts by weight Component composition:15~25 parts of chlorella pyrenoidosa (Chlorella pyrenoidosa) algae solution, goat's horn crescent moon algae 30~50 parts of (Selenastrum capricornutum) algae solution, Scenedesmus quadricauda (Scenedesmus quadricauda) algae solution 15~25 parts, 5~15 parts of photosynthetic bacteria (Photosynthetic bacteria) zymocyte liquid, Sphaerotilus natans (Sphaerotilusnatans) 10~20 parts of zymocyte liquid, thiobacillus denitrificans (Thiobacillus denitrificans) hair 10~20 parts of yeast-like fungi liquid, 5~15 parts of bafillus natto (Bacillus natto) zymocyte liquid, saccharomycete (Candida Santamariae) 5~15 parts of zymocyte liquid, 1~2 part of sodium humate;
Wherein, the concentration of the chlorella pyrenoidosa algae solution is 1.0 × 105~2.0 × 105Individual/mL;
The concentration of the goat's horn crescent moon algae algae solution is 1.0 × 105~3.0 × 105Individual/mL;
The concentration of the Scenedesmus quadricauda algae solution is 1.0 × 105~2.0 × 105Individual/mL;
It is living bacteria count in living bacteria count, the Sphaerotilus natans zymocyte liquid in the Fermentation by Photosynthetic Bacteria bacterium solution, described Living bacteria count, the yeast in living bacteria count, the bacillus natto to ferment bacterium solution in thiobacillus denitrificans zymocyte liquid In bacterium zymocyte liquid living bacteria count >=109Individual/mL.
2. the compound algae microbial inoculum according to claim 1 improved for culture-pool water quality, it is characterised in that its raw material It is made up of the component of following parts by weight:20 parts of chlorella pyrenoidosa algae solution, 40 parts of goat's horn crescent moon algae algae solution, Scenedesmus quadricauda algae solution 20 parts, 10 parts of Fermentation by Photosynthetic Bacteria bacterium solution, 15 parts of Sphaerotilus natans zymocyte liquid, 15 parts of thiobacillus denitrificans zymocyte liquid, natto 10 parts of fermentation of bacillus bacterium solution, 10 parts of saccharomycetes to make fermentation bacterium solution, 1 part of sodium humate zymocyte liquid;
Wherein, the concentration of the chlorella pyrenoidosa algae solution is 2.0 × 105Individual/mL;
The concentration of the goat's horn crescent moon algae algae solution is 2.0 × 105Individual/mL;
The concentration of the Scenedesmus quadricauda algae solution is 1.0 × 105Individual/mL;
Living bacteria count is 4.5 × 10 in the Fermentation by Photosynthetic Bacteria bacterium solution9Have in individual/mL, the Sphaerotilus natans zymocyte liquid It is 1.0 × 10 to imitate viable count10Living bacteria count is 3.0 × 10 in individual/mL, the thiobacillus denitrificans zymocyte liquid9Individual/mL, institute It is 3.5 × 10 to state living bacteria count in bafillus natto work zymocyte liquid9In individual/mL, the saccharomycetes to make fermentation bacterium solution effectively Viable count is 2.5 × 109Individual/mL.
3. the preparation method of the compound algae microbial inoculum according to claim 1 improved for culture-pool water quality, its feature It is, comprises the following steps:
Step 1, it is 1.0 × 10 chlorella pyrenoidosa culture to be tamed to concentration5~2.0 × 105Individual/mL, obtains the pyrenoids Chlorella algae solution;
It is 1.0 × 10 by goat's horn crescent moon algae culture domestication to concentration5~3.0 × 105Individual/mL, obtains the goat's horn crescent moon algae algae Liquid;
It is 1.0 × 10 that Scenedesmus quadricauda culture, which is tamed to concentration,5~2.0 × 105Individual/mL, obtains the Scenedesmus quadricauda algae solution;
Step 2, photosynthetic bacteria, Sphaerotilus natans, thiobacillus denitrificans, bafillus natto, saccharomycete are distinguished into fermented and cultured, obtained To respective zymocyte liquid;And living bacteria count, the Sphaerotilus natans zymophyte in the Fermentation by Photosynthetic Bacteria bacterium solution In liquid in living bacteria count, the thiobacillus denitrificans zymocyte liquid in living bacteria count, the bacillus natto to ferment bacterium solution In living bacteria count, the saccharomycetes to make fermentation bacterium solution living bacteria count >=109Individual/mL;
Step 3,15~25 parts of the chlorella pyrenoidosa algae solution, 30~50 parts of the goat's horn crescent moon algae algae solution, described four are weighed 15~25 parts of tail grid algae algae solution, 5~15 parts of the Fermentation by Photosynthetic Bacteria bacterium solution, the Sphaerotilus natans zymocyte liquid 10~20 Part, 10~20 parts of the thiobacillus denitrificans zymocyte liquid, 5~15 parts of the bacillus natto to ferment bacterium solution, the saccharomycete 5~15 parts of zymocyte liquid, 1~2 part of sodium humate, above-mentioned raw materials are mixed and stirred for uniformly, that is, to obtain the compound algae bacterium Agent.
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