CN103663728A - Composite microbial preparation for fresh-water aquiculture water body improvement - Google Patents
Composite microbial preparation for fresh-water aquiculture water body improvement Download PDFInfo
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- CN103663728A CN103663728A CN201310664803.2A CN201310664803A CN103663728A CN 103663728 A CN103663728 A CN 103663728A CN 201310664803 A CN201310664803 A CN 201310664803A CN 103663728 A CN103663728 A CN 103663728A
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Abstract
The invention provides a microbial preparation for fresh-water aquiculture water body improvement, which is prepared from the following raw materials in percentage by weight: 25-30 wt% of Bacillus subtilis, 25-30 wt% of Bacillus licheniformis and 15-20 wt% of Bacillus megaterium. The invention also provides a method for preparing the microbial preparation, which comprises the following steps: sequentially inoculating the Bacillus subtilis, Bacillus licheniformis and Rhodopseudomonas palustris seed culture solutions into a culture medium, fermenting to obtain a bacterial solution, and mixing the microbial solution with a carrier and nutrient substances to obtain the microbial preparation. The microbial preparation provided by the invention can obviously improve the quality of the culture water body and enhance the flesh texture of the cultured animals.
Description
Technical field
The present invention relates to a kind of microbial mixture, relate in particular to a kind of microbial preparation that can be used for poisons in freshwater improvement.
Background technology
China is the first in the world aquaculture big country, and according to statistics, Chinese inland can water surface for aquaculture be 2,734 ten thousand hectares, and seawater can cultured area 4,380,000 hectares, and rice field fish culture area calendar year 2001 is 1,520,000 hectares.Especially the southern china region of rivers and lakes, aquaculture occupies critical role in agriculture production, and many areas all produce the important channel of getting rich using aquaculture as local farmers, and aquaculture had become one of mainstay industry in cultivation agricultural already gradually.
Meanwhile, China is also that aquaculture water pollutes one of the most serious country.Just there is the saying of " breed fish and first support water " in China from ancient times, the quality of water quality is directly connected to the benefit of cultivation, and along with the development of aquaculture, high-density breeding need to drop into a large amount of feeds, bait remnants and aquatic animal movement pollute aquaculture water, cause there is NH
3, H
2the objectionable impurities such as S, organism increases, and chemical oxygen demand (COD) and biological oxygen demand (BOD) (BOD) increase.At present, the method that has multiple water treatment:
One, physical method
Machinery desilting is modal method, but mechanical desilting cost is high, and has scholar to think that silt from the bottom of lake is not source of pollution, and desilting only can improve water quality in a short time; It is also effective means that culturing pool is refreshed the water periodically, but cost is very high, and especially water resources is day by day in short supply, is difficult to carry out; Forward bottom current to upper strata, then discharge, can reduce Eutrophic Extent, but water waste is serious; Utilize gelifying agent, plastic film to cover bed mud, can reduce internal load, but can destroy water ecosystem; Exposure oxygenation can guarantee the oxygen amount that aquaculture organism is required, can prevent the amount reproduction of algae, but generally be only applicable to small water, and need to apply with other Combination of Methods simultaneously.In addition, patent CN101544412B discloses a kind of material that uses mineral material and modified product thereof to form, with objectionable impuritiess such as absorption ammonia nitrogen, phosphorus, organism and heavy metals, but this patent is used a large amount of organic modifiers, other pollutent can be incorporated in water body.
Two, chemical process
Chemical agent can in form microprotein halfcystine-SH radical reaction, the enzymatic inactivation that make-SH base is active site, thereby the cell walls of destroy microorganisms, cytolemma and internal substance, thus kill cell, and at present conventional have copper sulfate, copper chelate, chlorine or an oxychloride etc.; As patent CN102730783A discloses the aquaculture water modifying agent that aluminum chloride, Tai-Ace S 150, gac form, in addition, patent CN101481170A also discloses thionamic acid at degrading nitrite and has reduced the application in water pH value.But aquaculture water and environment are subject to severe contamination in recent years, the situation of aquatic products medication is more and more serious, causes pathogenic bacterium to produce resistance, and aquaculture organism medicine residual exceeds standard, and to human consumer, brings some diseases.
Three, biological regulation and control and biological restoration
Utilize microbial preparation can improve water quality, reduce and control the content of ammonia, nitrite in aquaculture water, and can control pathogenic micro-organism Growth and reproduction, to improve the resistibility of animal, biotechnological formulation is harmless, have no side effect, the continuous action time is long, and " beneficial microorganism " or " probiotics " is otherwise known as.The application of microbial preparation in aquatic products is at present more and more extensive.
Commercial applications is photosynthetic bacterium the earliest, for regulating water quality, then, on this basis, in succession develops again multiple-microorganism, and the mixed culture of microorganism and synergy also more and more receive people's concern.Xue Heng equality (fodder industry, 1997,18 (2): 23-26) use genus bacillus, photosynthetic bacterium and Bdellovibrio composite fungus agent, discovery prawn virus disease is sent out time lag; (aquatic science and technology information, 1996,23 (2): 51-55) photosynthetic bacterium is applied to together with actinomycetes and supports soft-shelled turtle pond, COD clearance can reach more than 90% such as Ye Yizuo; Official (Chinese aquatic science, 2000,7 (2): 51-55) with beautiful, build bacterium and to supporting soft-shelled turtle pond, carry out water quality improvement with photosynthetic bacterium, can increase dissolved oxygen, reduction COD and ammonia nitrogen such as promote culture; Luo Yongsheng etc. (agro-environment science journal, 2006,25 volume supplementary issues: 206-210) use photosynthetic bacterium and genus bacillus, degradable water body COD, the two synergy is obviously; Patent CN1266054C discloses the mixture of a kind of composite fungus agent and micro-algae, and bacterial classification comprises bacillus megaterium, nitrobacteria, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, actinomycetes, streptomycete, genus bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria; Patent CN1226202C discloses a kind of microbial preparation for purifying water body, comprises nitrobacteria, nitrite bacteria, denitrifying bacterium, sulfuration bacterium, photosynthetic bacterium.
But, still need the new microbial preparation for aquaculture water improvement at present.
Summary of the invention
In order to improve the water quality of aquaculture water, and improve the meat of cultivated animals, the invention provides a kind of microorganism system for the improvement of freshwater aquiculture water body.
First aspect of the present invention is to provide a kind of microorganism system for the improvement of freshwater aquiculture water body, and according to weight percent, raw material comprises:
Subtilis 25-30wt%;
Bacillus licheniformis 25-30wt%;
Bacillus megaterium 15-20wt%.
Microorganism system for the improvement of freshwater aquiculture water body of the present invention, can also contain the microorganism that other can be used for the improvement of freshwater aquiculture water body, but be preferably:
Microorganism system for the improvement of freshwater aquiculture water body of the present invention, microorganism is preferably by subtilis, Bacillus licheniformis and bacillus megaterium and forms, the gross weight of described microorganism system of take is benchmark, and the weight percent of subtilis, Bacillus licheniformis and bacillus megaterium is respectively 25-30wt%, 25-30wt%, 15-20wt%.
Microorganism system for the improvement of freshwater aquiculture water body of the present invention, be preferably >=3,000,000,000/g of viable count.
Microorganism system for the improvement of freshwater aquiculture water body of the present invention, can be liquid preparation or solid preparation, therefore can also comprise carrier, and described carrier can be liquid vehicle, as water; Also can be solid carrier, as any one or a few the mixture in zeolite, calcium carbonate, kaolin (comprising calcined kaolin), wilkinite.
The microorganism system of freshwater aquiculture water body of the present invention improvement is preferably solid preparation, and is preferably particle and/or powder, and median size is preferably 10-100 order, 20-80 order more preferably, and 30-70 order more preferably, 40-60 order more preferably, as 50 orders.
Any one above-mentioned microorganism system improveing for freshwater aquiculture water body of the present invention is preferably solid preparation.
Microorganism system for the improvement of freshwater aquiculture water body of the present invention, can also comprise nutritive substance, described nutritive substance can be any one or a few the mixture being selected from starch (being preferably W-Gum), molasses, trehalose, sucrose, maltose, isomaltose, wood sugar, fructose, stachyose, raffinose.
According to a kind of preferred embodiment of microorganism system of the present invention, the gross weight of carrier (being preferably solid carrier) and nutritive substance of take is benchmark, and the weight percent of carrier is 80-90w%, and the weight percent of nutritive substance is 10-20wt%.
In the preferred embodiment of microorganism system of the present invention, described carrier is the combination of zeolite powder and calcium carbonate, and described nutritive substance is the combination of W-Gum and molasses; Wherein, zeolite powder weight percent is 65-75wt%, and weight of calcium carbonate per-cent is 10-20wt%; The weight percent of W-Gum is 5-15wt%, and the weight percent of molasses is 1-10wt%.
Second aspect of the present invention is to provide a kind of method of preparing the above-mentioned microorganism system for the improvement of freshwater aquiculture water body, and described method comprises the steps:
Step 1, provides subtilis, Bacillus licheniformis and bacillus megaterium seed culture fluid;
Step 2, subtilis, Bacillus licheniformis and bacillus megaterium seed culture fluid are inoculated in substratum successively, fermentation, the then concentrated high-concentration bacterial liquid that obtains;
Step 3, gained high-concentration bacterial liquid mixes with carrier, nutritive substance, obtains described microbial preparation.
In step 1, the preparation method of subtilis, Bacillus licheniformis seed culture fluid is preferably respectively and cultivates under 30-35 ℃ of condition, obtains described seed culture fluid.The water that culturing process culture medium matrix component used is preferably by molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium-chlor 0.2-0.25wt%, ferric sulfate 30-35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% and surplus forms, and the pH value of substratum is 6.5-6.8.
The incubation time of subtilis, Bacillus licheniformis is preferably 36-54 hour, 40-55 hour more preferably, and 45-50 hour more preferably, as 47 hours, 48 hours.
In step 1, it is to cultivate under 4500-10000lx condition that the preparation method of bacillus megaterium seed culture fluid is preferably 29-33 ℃ and intensity of illumination, obtains described seed culture fluid.Culturing process culture medium matrix component used is preferably by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, the water of peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt% and surplus forms, and the pH value of substratum is 6.5-6.8.
The incubation time of bacillus megaterium is preferably 5-10 days, more preferably 6-9 days, more preferably 6-8 days.
In step 2, described nutrient media components is preferably by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, the water of peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt% and surplus forms, and pH value is 6.5-6.8.
Wherein, in foregoing, growth factor solution is preferably and comprises the vitamin H aqueous solution of 2-2.3wt% mass concentration, the vitamins B aqueous solution of 12-15wt% mass concentration.Described vitamins B can be any one or any multiple mixture in vitamin B group.
In step 2, in described seeded process, inoculum size is preferably the 2%-5% of substratum gross weight described in step 2.
In step 2, in described fermenting process, temperature is preferably arbitrary temp or the temperature range being controlled within the scope of 35-40 ℃.
In step 2, it is to carry out under 200r/min-280r/min, the air flow condition that is 1: 1 that described fermenting process is preferably at mixing speed.
In step 2, described fermenting process, is preferably and continues 30-50 hour, more preferably 33-46 hour, more preferably 36-42 hour.
In step 2, described concentrated being preferably by centrifugation undertaken, and the rotating speed of centrifugation is preferably 3000-5000r/min.
In second aspect of the present invention, described carrier is solid carrier, as any one or a few the mixture in zeolite, calcium carbonate, kaolin, wilkinite.
In second aspect of the present invention, described nutritive substance can be any one or a few the mixture being selected from starch (being preferably W-Gum), molasses, trehalose, sucrose, maltose.
The first preferred embodiment of second aspect according to the present invention, the gross weight of carrier (being preferably solid carrier) and nutritive substance of take is benchmark, and the weight percent of carrier is 70-90w%, and the weight percent of nutritive substance is 10-30wt%.
In the first preferred embodiment of second aspect of the present invention, described carrier is zeolite powder and calcium carbonate, and described nutritive substance is W-Gum and molasses; Wherein, zeolite powder weight percent is 60-70wt%, and weight of calcium carbonate per-cent is 10-20wt%; The weight percent of W-Gum is 5-15wt%, and the weight percent of molasses is 5-15wt%.
The second preferred embodiment of second aspect according to the present invention, the gross weight of described microorganism system of take is benchmark, the weight percent of subtilis, Bacillus licheniformis and bacillus megaterium is respectively 15-40wt%, 15-40wt%, 10-20wt%.
According to the present invention, the third preferred embodiment of second aspect, in step 3, is dried after granulation, and is preferably that to be dried to moisture content be 5-7%.
The 4th of second aspect the kind of preferred embodiment according to the present invention, also comprises step 4: step 3 gained mixture is pulverized, and the temperature in crushing process is controlled at arbitrary temp or the temperature range within the scope of 10-45 ℃.
In the 4th kind of preferred embodiment aspect second of the present invention, after pulverizing, can also comprise the step of screening, described screening is preferably and adopts 10-100 mesh sieve to implement, 20-80 mesh sieve more preferably, 30-70 mesh sieve more preferably, 40-60 mesh sieve more preferably, as 50 mesh sieves.
Microbial preparation for the improvement of freshwater aquiculture water body provided by the invention, can fast reducing water body BOD, ammonia-nitrogen content, nitrite content and pH value, increase dissolved oxygen amount, can realize in breeding process and throwing in, and can improve the meat of cultivated animals.
The method of the described microbial preparation of preparation provided by the invention, can access the microbial preparation of high viable bacteria content, and preparation method is simple, and the kind of required bacterial classification is few, and cost is low.
Embodiment
Embodiment 1
Raw material forms:
Subtilis 25wt%
Bacillus licheniformis 30wt%
Bacillus megaterium 15wt%
Carrier and nutritive substance 30wt%
Preparation method:
Step 1:
Respectively subtilis and Bacillus licheniformis are inoculated in the first substratum under aseptic condition, 30 ℃-35 ℃, single spawn culture 48 hours, obtains subtilis and Bacillus licheniformis seed culture fluid, and its viable count is all greater than 5.0 * 10
9cfu/ml.Wherein, the first substratum is comprised the aqueous solution of vitamin H 2-2.3wt%, vitamin B12-15wt% by molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium-chlor 0.2-0.25wt%, ferric sulfate 30-35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt%() and the water of surplus form, pH value is 6.5-6.8.
Bacillus megaterium is inoculated under aseptic condition in the second substratum, intensity of illumination is that 4500-10000lx, temperature are under 29 ℃ of-33 ℃ of conditions, cultivates 6-8 days, obtains bacillus megaterium seed culture fluid, and its viable count is greater than 5.0 * 109cfu/ml.Wherein, the second substratum is by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt%(comprise the aqueous solution of vitamin H 2-2.3wt%, vitamin B complexes 12-15wt%) and the water of surplus form, pH value is 6.5-6.8.
Step 2,
The subtilis seed culture fluid obtaining in step 1, Bacillus licheniformis seed culture fluid, bacillus megaterium seed culture fluid are inoculated into successively in proportion in the 3rd substratum under aseptic condition, and inoculum size is the 2%-5% of this substratum total amount.
At 35-40 ℃, mixing speed, be under 200r/min-280r/min, the air flow condition that is 1: 1, fermentation 36-42 hour, the viable count that obtains subtilis is greater than 3.0 * 10
9the viable count of cfu/ml, Bacillus licheniformis is greater than 3.0 * 10
9the viable count of cfu/ml, bacillus megaterium is greater than 1.0 * 10
9the fermented liquid of cfu/ml; By fermented liquid high speed centrifugation under the rotating speed of 3000-5000r/min, obtain the concentrated bacterium liquid of high density as mother liquor.
Wherein, the 3rd substratum is by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt%(comprise the aqueous solution of vitamin H 2-2.3wt%, vitamin B complexes 12-15wt%) and the water of surplus form, pH value is 6.5-6.8.
Step 3,
To in the concentrated bacterium liquid immigration matrix that for zeolite powder+feed, calcium carbonate+W-Gum+molasses form, mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, matrix (carrier and the nutritive substance) gross weight of take is benchmark, and matrix is comprised of zeolite powder 70wt%, calcium carbonate 15wt%, W-Gum 10wt%, molasses 5wt%.
Step 4,
By the material obtaining in step 3 in temperature not higher than 45 ℃ and be not less than under the condition of 10 ℃, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, the microbial preparation for the improvement of freshwater aquiculture water body that to obtain moisture content≤6%, viable count be 3,000,000,000/g.
Embodiment 2
Raw material forms:
Subtilis 30wt%
Bacillus licheniformis 20wt%
Bacillus megaterium 20wt%
Carrier and nutritive substance 30wt%
Preparation method:
Step 1:
According to the method described in embodiment 1, provide subtilis seed culture fluid, Bacillus licheniformis seed culture fluid, bacillus megaterium seed culture fluid.
Step 2,
According to the method described in embodiment 1, obtain the concentrated bacterium liquid of high density as mother liquor.
Step 3,
To in the concentrated bacterium liquid immigration matrix that for zeolite powder+feed, calcium carbonate+W-Gum+molasses form, mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, matrix (carrier and the nutritive substance) gross weight of take is benchmark, and matrix is comprised of zeolite powder 75wt%, calcium carbonate 10wt%, W-Gum 8wt%, molasses 7wt%.
Step 4,
By the material obtaining in step 3 in temperature not higher than 45 ℃ and be not less than under the condition of 10 ℃, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, the microbial preparation for the improvement of freshwater aquiculture water body that to obtain moisture content≤6%, viable count be 3,000,000,000/g.
Embodiment 3
Raw material forms:
Subtilis 25wt%
Bacillus licheniformis 25wt%
Bacillus megaterium 15wt%
Carrier and nutritive substance 35wt%
Preparation method:
Step 1:
According to the method described in embodiment 1, provide subtilis seed culture fluid, Bacillus licheniformis seed culture fluid, bacillus megaterium seed culture fluid.
Step 2,
According to the method described in embodiment 1, obtain the concentrated bacterium liquid of high density as mother liquor.
Step 3,
To in the concentrated bacterium liquid immigration matrix that for zeolite powder+feed, calcium carbonate+W-Gum+molasses form, mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, matrix (carrier and the nutritive substance) gross weight of take is benchmark, and matrix is comprised of zeolite powder 65wt%, calcium carbonate 15wt%, W-Gum 5wt%, molasses 5wt%.
Step 4,
By the material obtaining in step 3 in temperature not higher than 45 ℃ and be not less than under the condition of 10 ℃, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, obtains the microbial preparation for the improvement of freshwater aquiculture water body that moisture content≤6%, viable count are about 3,000,000,000/g.
Embodiment 4
Raw material forms:
Subtilis 28wt%
Bacillus licheniformis 26wt%
Bacillus megaterium 17wt%
Carrier and nutritive substance 29wt%
Preparation method:
Step 1:
According to the method described in embodiment 1, provide subtilis seed culture fluid, Bacillus licheniformis seed culture fluid, bacillus megaterium seed culture fluid.
Step 2,
According to the method described in embodiment 1, obtain the concentrated bacterium liquid of high density as mother liquor.
Step 3,
To in the concentrated bacterium liquid immigration matrix that for zeolite powder+feed, calcium carbonate+W-Gum+molasses form, mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, matrix (carrier and the nutritive substance) gross weight of take is benchmark, and matrix is comprised of zeolite powder 70wt%, calcium carbonate 10wt%, W-Gum 10wt%, molasses 10wt%.
Step 4,
By the material obtaining in step 3 in temperature not higher than 45 ℃ and be not less than under the condition of 10 ℃, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, obtains the microbial preparation for the improvement of freshwater aquiculture water body that moisture content≤6%, viable count are about 3,000,000,000/g.
Claims (10)
1. for a microbial preparation for freshwater aquiculture water body improvement, it is characterized in that, according to weight percent, raw material comprises:
Subtilis 25-30wt%;
Bacillus licheniformis 25-30wt%;
Bacillus megaterium 15-20wt%.
2. microbial preparation according to claim 1, is characterized in that, also comprises carrier and/or nutritive substance.
3. microbial preparation according to claim 2, is characterized in that, described carrier is selected from any one or a few the mixture in zeolite, calcium carbonate, kaolin, wilkinite.
4. microbial preparation according to claim 2, is characterized in that, described nutritive substance is selected from any one or a few the mixture in starch, molasses, trehalose, sucrose, maltose, isomaltose, wood sugar, fructose, stachyose, raffinose.
5. according to the method described in any one in claim 2-4, it is characterized in that, take carrier and nutritive substance gross weight is benchmark, and the weight percent of carrier is 80-90w%, and the weight percent of nutritive substance is 10-20wt%.
6. method according to claim 5, is characterized in that, described carrier is the combination of zeolite powder and calcium carbonate, and described nutritive substance is the combination of W-Gum and molasses; Wherein, zeolite powder weight percent is 65-75wt%, and weight of calcium carbonate per-cent is 10-20wt%; The weight percent of W-Gum is 5-15wt%, and the weight percent of molasses is 5-15wt%.
7. prepare a method for microbial preparation as claimed in claim 1, it is characterized in that, comprise the steps: step 1, subtilis, Bacillus licheniformis and bacillus megaterium seed culture fluid are provided;
Step 2, subtilis, Bacillus licheniformis and bacillus megaterium seed culture fluid are inoculated in substratum successively, fermentation, the then concentrated high-concentration bacterial liquid that obtains;
Step 3, gained high-concentration bacterial liquid mixes with carrier, nutritive substance, obtains described microbial preparation.
8. method according to claim 7, it is characterized in that, described in step 2, nutrient media components is by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, the water of peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt% and surplus forms, and pH value is 6.5-6.8; Wherein, growth factor solution comprises the vitamin H aqueous solution of 2-2.3wt% mass concentration, the vitamins B aqueous solution of 12-15wt% mass concentration.
9. method according to claim 8, is characterized in that, in fermenting process, temperature is controlled at arbitrary temp or the temperature range within the scope of 35-40 ℃ described in step 2.
10. method according to claim 7, is characterized in that, step also comprises:
Step 4, pulverizes step 3 gained mixture, and the temperature in crushing process is controlled at arbitrary temp or the temperature range within the scope of 10-45 ℃.
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| CN104099268A (en) * | 2014-06-17 | 2014-10-15 | 贝嘉美（天津）生物技术研发有限公司 | Aquaculture composite microbial agent |
| CN104312949A (en) * | 2014-10-10 | 2015-01-28 | 苏州市环境科学研究所 | Organic chloride pollutant purifying method as well as purifying agent and culture solution used by method |
| CN104446708A (en) * | 2014-12-04 | 2015-03-25 | 成都新朝阳作物科学有限公司 | Microbial fertilizer as well as preparation method and purpose thereof |
| CN105002119A (en) * | 2015-08-17 | 2015-10-28 | 海南卓越生物有限公司 | Semisolid fungus cultivation method for nitrifying bacteria |
| CN105309342A (en) * | 2014-07-29 | 2016-02-10 | 上海绿奥生物科技有限公司 | Methods for increasing live young shrimp quantity of young shrimp breeding and breeding yield of Penaeus vannamei Boone |
| CN105907690A (en) * | 2016-07-06 | 2016-08-31 | 苏州市相城区阳澄湖镇剑美水产生态养殖专业合作社 | Microbial preparation for aquiculture |
| CN108587956A (en) * | 2018-04-25 | 2018-09-28 | 正丰源生物科技（苏州）有限公司 | A kind of composite bacterial fertilizer for sewage disposal |
| CN111349627A (en) * | 2018-12-24 | 2020-06-30 | 中粮生物化学（安徽）股份有限公司 | Microbial agent carrier, microbial solid microbial agent and preparation method thereof |
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