CN104099268A - Aquaculture composite microbial agent - Google Patents
Aquaculture composite microbial agent Download PDFInfo
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- CN104099268A CN104099268A CN201410271491.3A CN201410271491A CN104099268A CN 104099268 A CN104099268 A CN 104099268A CN 201410271491 A CN201410271491 A CN 201410271491A CN 104099268 A CN104099268 A CN 104099268A
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Abstract
The invention relates to an aquaculture composite microbial agent, which comprises the following agents by weight: 5-10 parts of Bacillus subtilis, 0.5-4 parts of Bacillus licheniformis, 1-6 parts of bacillus megaterium, 0.2-1 part of Pseudomonas gelatinasa, 0.5-3 parts of Rhodopseudomonas capsulata, and 2-8 parts of Bdellovibrio bacteriovorus. The invention also relates to application of the aquaculture composite microbial agent in improvement of water quality and/or control of aquatic organism diseases.
Description
Technical field
The present invention relates to aquaculture field, be specifically related to a kind of aquaculture complex micro organism fungicide and application thereof.
Background technology
China is in the world one of larger aquaculture big country, and in the many areas of China, aquaculture becomes the important source of finance of local farmers.Since intensive aquaculture pattern is since China is flourish, solved the problem of natural fishing resources relative shortage, but the water body environments such as the most of rivers of while Ye Dui China, lake have caused very large pressure, are seriously restricting the Sustainable development of China's culture fishery.For example, a large amount of dissolved oxygens of organic substance decomposing consumption such as biological remains, cause bottom anoxic, discharge some objectionable impuritiess simultaneously, as ammonia nitrogen, nitrite, sulfide etc. make substrate, worsen.
In aquaculture industry, mainly by the method for physics, chemistry, biological-ecological, cultivation water environment is improved and repaired at present, and derive thus Series of Water quality improving agent product.Chemical restoration is by chemical reaction, to be eliminated or reduced a kind of method of pollutant in water, although the effect of chemical classes product rapidly, but lacks long-lasting, and some products also can form secondary pollution, and some use costs are higher.
Biological restoration utilizes specific biology to eliminate or enrichment environment pollutent exactly under certain condition, thereby plays the reparation of polluted water environment and improving effect.Utilize beneficial microorganism to carry out microorganism regulation and control to cultivation water environment, purify water, be more and more subject to people's attention.Various microorganisms are progressively applied at aspects such as water quality improvements.These microorganisms can be degraded rapidly and be entered the organism of culturing pool, effectively reduce ammonia nitrogen in water, sulfide, nitrous acid nitrogen, COD etc., thereby effectively improve water quality, maintain good cultivation water area ecological environment.
CN103601302A compound subtilis slow releasing tablet, its component comprises: the former powder 2～9% of subtilis, SPC-D 73～96%, sustained release dosage 1～10%, stablizer 1～8%.Described tablet comprises SPC-D, this not only can fast activating subtilis, promote the growth and reproduction of subtilis, and can increase the dissolved oxygen in cultivation water environment, solved the water hypoxia causing because of subtilis amount reproduction oxygen consumption.
CN103224886A discloses a kind of used for aquiculture complex micro organism fungicide, this microbial inoculum, by bacillus laterosporus, Bacillus licheniformis, streptococcus faecium, subtilis and five floras of yeast, is mixed according to the weight ratio of 1～4:2～5:0.8～2:2～4:0.2～1.Described product can rapid subsidence to water bottom, the organic pollutants such as the residual bait in rapid degrading cultivation water, ight soil, biological residual body, effectively improve water bottom environment, and reduce aquaculture water ammonia nitrogen, nitrite content, can also improve the immunizing power of aquatic animal simultaneously.
Yet the present inventor attempts to find and a kind ofly can efficiently improve aquifer cultivation environment, the composite fungus agent of purifying water body, contriver has passed through a large amount of screenings and application on the spot, has obtained complex micro organism fungicide formula of the present invention.In addition, in application process, contriver also finds that described complex micro organism fungicide is when effectively improving aquifer cultivation environment, can obviously reduce hydrobiological sickness rate.Based on above-mentioned discovery, contriver has completed the present invention.
Summary of the invention
One aspect of the present invention relates to a kind of aquaculture complex micro organism fungicide, and it comprises following microbial inoculum and weight part thereof:
Preferably, in described aquaculture complex micro organism fungicide, the weight part of each microbial inoculum is:
Further preferred, in described aquaculture complex micro organism fungicide, the weight part of each microbial inoculum is:
Most preferred, in described aquaculture complex micro organism fungicide, the weight part of each microbial inoculum is:
Or the weight part of each microbial inoculum is in described aquaculture complex micro organism fungicide:
In above-mentioned arbitrary described aquaculture complex micro organism fungicide, the number of viable of each microbial inoculum can be:
Preferably, in above-mentioned arbitrary described aquaculture complex micro organism fungicide, the number of viable of each microbial inoculum is:
Preferred, in above-mentioned arbitrary described aquaculture complex micro organism fungicide, the number of viable of each microbial inoculum is:
Aquaculture complex micro organism fungicide of the present invention can improve water body color, increases water transparency, reduce ammonia-nitrogen content and COD, can also effectively reduce hydrobiological sickness rate, such as can effectively reducing catfish disease as catfish putrid skin disease, catfish white skin disease, catfish black matrix disease etc. simultaneously.
The present invention relates to the preparation that comprises aforementioned arbitrary described aquaculture complex micro organism fungicide on the other hand, wherein can in above-mentioned complex micro organism fungicide, add suitable auxiliary material to be prepared into pulvis, tablet, granula and capsule.Described preparation method and required auxiliary material are known.For example aquaculture complex micro organism fungicide can be prepared into pulvis, concrete grammar mixes standby for taking the above-mentioned aquaculture complex micro organism fungicide of formula ratio, take afterwards appropriate filler and pulverize, then above-mentioned aquaculture complex micro organism fungicide mixture is further mixed with the filler of pulverizing.For example, while being prepared into tablet or granula, can in the above-mentioned pulvis being mixed to get, further add suitable tackiness agent, through compressing tablet or granulation.Wherein filler used is such as being diatomite, kaolin, clay, attapulgite etc.Tackiness agent used is such as being starch, polyvinyl alcohol, carboxymethyl cellulose, polyoxyethylene glycol etc.
The present invention relates to aforementioned aquaculture complex micro organism fungicide or its preparation on the other hand for improveing the application of water quality and/or control aquatic living things disease.
Preferably, the present invention relates to described aquaculture complex micro organism fungicide or its preparation for improving the application of water body color, increase water transparency, reduction ammonia-nitrogen content and COD.
Or, the present invention relates to described aquaculture complex micro organism fungicide or its preparation for reducing the application of disease generation in catfish cultivation.Wherein said disease is catfish putrid skin disease, catfish white skin disease, catfish black matrix disease.
Most preferred, the present invention relates to described aquaculture complex micro organism fungicide or its preparation for improving water body color, increase water transparency, reducing ammonia-nitrogen content and COD, and can reduce the application that in catfish cultivation, disease occurs simultaneously, wherein said disease is catfish putrid skin disease, catfish white skin disease, catfish black matrix disease.
In an application of the invention, the consumption of aquaculture complex micro organism fungicide of the present invention or its preparation makes microorganism live bacteria described in final water body add up to 10-10
6cFU/g, preferred 100-10
5cFU/g, more preferably 100-10
4cFU/g.
The various microbiobacterial agents that the present invention is used and auxiliary material are all commercially available.For example, described microbiobacterial agent can be purchased from the industrial Bioisystech Co., Ltd in Cangzhou, Beijing Hua Nong biotechnology company limited, Lv Hui bio tech ltd, Guangzhou etc.Described auxiliary material as diatomite, kaolin, clay, attapulgite, starch, polyvinyl alcohol, carboxymethyl cellulose, polyoxyethylene glycol etc. be all also on market, hold facile.
Beneficial effect of the present invention shows:
1. purify water: the various hazardous and noxious substances that can degrade rapidly in water body, improve water colour, improve water transparency, optimize algae and probiotics growth and breeding, suppress the growth and breeding of unwanted bacteria, promote the eubiosis of water surrounding.
2. efficiently decompose: have powerful capacity of decomposition, can decompose rapidly the animal excrement in water body, residual bait, dead algae, dead moss, animals and plants corpse, pool bottom sludge etc., eliminate hazardous and noxious substances.
3. improve environment: can accelerate water ecology circulation, improve water transparency, reduce ammonia nitrogen, nitrite, hydrogen sulfide, regulate pH value, balance water nutrition, improves efficiency of feed utilization, changes pond also can play rapidly improved effect for aging difficulty.
4. promote cultivation: can suppress the growth and breeding of pathogenic bacteria, strengthen the physique of producing animal, reduce morbidity, promote growth, improve culture benefit.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, but does not limit the invention to these embodiments.One skilled in the art would recognize that the present invention contained all alternativess, improvement project and the equivalents that within the scope of claims, may comprise.
Microbial inoculum number of viable used in embodiment is: approximately 18,000,000,000/g of subtilis, approximately 10,000,000,000/g of Bacillus licheniformis, approximately 5,500,000,000/g of bacillus megaterium, approximately 4,000,000,000/g of colloid rhodopseudomonas, approximately 7,000,000,000/g of capsula Rhodopseudomonas, approximately 12,000,000,000/g of bacteriophagic Bdellovibrio.
(1) FORMULATION EXAMPLE
Embodiment 1
Take subtilis powder 8kg, Bacillus licheniformis powder 2kg, bacillus megaterium powder 3kg, colloid rhodopseudomonas powder 0.6kg, capsula Rhodopseudomonas powder 1kg, bacteriophagic Bdellovibrio powder 5kg, mixed, obtain bacterium powder mixture.Take 80kg diatomite, more than being crushed to granularity 100 orders.Afterwards bacterium powder mixture obtained above is added in the diatomite through pulverizing, mix, obtain aquaculture complex micro organism fungicide pulvis of the present invention.
Embodiment 2
Take subtilis powder 7kg, Bacillus licheniformis powder 1kg, bacillus megaterium powder 4kg, colloid rhodopseudomonas powder 0.7kg, capsula Rhodopseudomonas powder 2kg, bacteriophagic Bdellovibrio powder 4kg, mixed, obtain bacterium powder mixture.Take 75kg attapulgite, more than being crushed to granularity 100 orders.Afterwards bacterium powder mixture obtained above is added in the attapulgite through pulverizing, mix.Take 3kg polyvinyl alcohol, add suitable quantity of water to be made into binder aqueous solution, and stirring limit, limit is evenly sprayed to above-mentioned binder aqueous solution in the above-mentioned pulvis through mixing; Sprinkling completes by extruder grain, obtains aquaculture complex micro organism fungicide granula of the present invention.
Embodiment 3
Take subtilis powder 10kg, Bacillus licheniformis powder 1kg, bacillus megaterium powder 5kg, colloid rhodopseudomonas powder 1kg, capsula Rhodopseudomonas powder 0.5kg, bacteriophagic Bdellovibrio powder 7kg, mixed, obtain bacterium powder mixture.Take 70kg kaolin, more than being crushed to granularity 100 orders.Afterwards bacterium powder mixture obtained above is added in the kaolin through pulverizing, mix.Take 3kg starch, add suitable quantity of water to be made into binder aqueous solution, and stirring limit, limit is evenly sprayed to above-mentioned binder aqueous solution in the above-mentioned pulvis through mixing; Sprinkling completes by granulation, compressing tablet, obtains aquaculture complex micro organism fungicide tablet of the present invention.
Embodiment 4
Take subtilis powder 6kg, Bacillus licheniformis powder 4kg, bacillus megaterium powder 2kg, colloid rhodopseudomonas powder 1kg, capsula Rhodopseudomonas powder 3kg, bacteriophagic Bdellovibrio powder 3kg, mixed, obtain bacterium powder mixture.Take 80kg attapulgite, more than being crushed to granularity 100 orders.Afterwards bacterium powder mixture obtained above is added in the attapulgite through pulverizing, mix, obtain aquaculture complex micro organism fungicide pulvis of the present invention.
Comparative example 1
Take subtilis powder 20kg standby.Take 80kg attapulgite, more than being crushed to granularity 100 orders.Afterwards above-mentioned subtilis powder is added in the attapulgite through pulverizing, mix, obtain.
Comparative example 2
Take subtilis powder 10kg, Bacillus licheniformis powder 10kg, mixed, obtain bacterium powder mixture.Take 80kg attapulgite, more than being crushed to granularity 100 orders.Afterwards bacterium powder mixture obtained above is added in the attapulgite through pulverizing, mix, obtain.
Comparative example 3
Take bacillus megaterium powder 12kg, colloid rhodopseudomonas powder 8kg, mixed, obtain bacterium powder mixture.Take 80kg attapulgite, more than being crushed to granularity 100 orders.Afterwards bacterium powder mixture obtained above is added in the attapulgite through pulverizing, mix, obtain.
Comparative example 4
Take capsula Rhodopseudomonas powder 7kg, bacteriophagic Bdellovibrio powder 13kg, mixed, obtain bacterium powder mixture.Take 80kg attapulgite, more than being crushed to granularity 100 orders.Afterwards bacterium powder mixture obtained above is added in the attapulgite through pulverizing, mix, obtain.
(2) paddy field test
Test paddy field: Tianjin production of catfish, approximately 10 mu of culture pond water surface areas;
Breed variety: catfish (southern catfish);
Test method: choose the more consistent culture pond of water quality, with the microbial inoculum preparation of embodiment 1-4 and reference examples 1-4, each culture pond is processed respectively in season in catfish cultivation, blank is left intact.Each consumption of processing microbial inoculum is that the viable count summation that makes to use microbiobacterial agent described in rear culture pond water body is 500-1000CFU/g, and guarantees that each viable count of processing is consistent.
Process, after processing, each variation of water of processing of investigation in a week, the results are shown in following table 1 for each.
In addition, process, in processing, within latter 15 days, choose immediately respectively 500 catfish, investigation catfish white skin disease incidence, the results are shown in following table 2 for each.
Each processes water-quality guideline variation before and after processing table 1
From the data of table 1, be not difficult to find out, aquaculture complex micro organism fungicide of the present invention is with respect to contrast microbial inoculum and blank, no matter be water body color, transparency, ammonia-nitrogen content, COD also or in water body, all have clear improvement.Concrete, with after aquaculture complex micro organism fungicide processing of the present invention, water body color from dark green become light green, water transparency increase is greater than 10cm, be up to 18cm, and the processing of contrast microbial inoculum has water body color substantially unchanged, and water transparency increasing degree is less.Secondly, after processing with aquaculture complex micro organism fungicide of the present invention, water body ammonia-nitrogen content reduces greatly, and for example, in using the processing of embodiment 2, before processing, in water body, ammonia-nitrogen content is 0.74mg/L, is down to 0.1mg/L after processing, and rangeability is obvious.And after processing with contrast microbial inoculum, water body ammonia-nitrogen content changes not obvious.Moreover after processing with aquaculture complex micro organism fungicide of the present invention, water body COD reduces greatly, for example, in using the processing of embodiment 2, before processing, water body COD is 46.3mg/L, is down to 7.7mg/L after processing, and rangeability is obvious.And after processing with contrast microbial inoculum, water body COD changes not obvious.
Table 2 is respectively processed the investigation of catfish incidence
| Process | Investigation sum (bar) | Morbidity number (bar) | Sickness rate | Sickness rate reduces (with respect to blank) |
| Embodiment 1 | 500 | 9 | 1.80％ | 7.20％ |
| Embodiment 2 | 500 | 8 | 1.60％ | 7.40％ |
| Embodiment 3 | 500 | 12 | 2.40％ | 6.60％ |
| Embodiment 4 | 500 | 14 | 2.80％ | 6.20％ |
| Comparative example 1 | 500 | 35 | 7.00％ | 2.00％ |
| Comparative example 2 | 500 | 35 | 7.00％ | 2.00％ |
| Comparative example 3 | 500 | 36 | 7.20％ | 1.80％ |
| Comparative example 4 | 500 | 38 | 7.60％ | 1.40％ |
| Blank | 500 | 45 | 9.00％ | - |
From the data of table 2, be not difficult to find out, after processing with aquaculture complex micro organism fungicide of the present invention, the sickness rate of culture pond catfish obviously reduces, and this illustrates that microbial inoculum of the present invention can effectively prevent and/or treat catfish disease.Concrete, for example, in using the processing of embodiment 2, its catfish sickness rate is 1.6%, this is starkly lower than the sickness rate 9% of blank, and is starkly lower than the sickness rate after processing with contrast microbial inoculum.
Above-mentioned example is only to further explanation of the present invention, it is not limitation of the invention, by the scheme that such scheme is simply adjusted and then obtained, its relevant change does not all depart from content of the present invention, within being all deemed to be included in scope of the present invention.
Claims (10)
1. an aquaculture complex micro organism fungicide, it comprises following microbial inoculum and weight part thereof:
2. aquaculture complex micro organism fungicide according to claim 1, is characterized in that, comprises following microbial inoculum and weight part thereof:
3. aquaculture complex micro organism fungicide according to claim 1, is characterized in that, comprises following microbial inoculum and weight part thereof:
4. according to the arbitrary described aquaculture complex micro organism fungicide of claim 1-3, it is characterized in that, in described aquaculture complex micro organism fungicide, the number of viable of each microbial inoculum is:
5. aquaculture complex micro organism fungicide according to claim 4, is characterized in that, in described aquaculture complex micro organism fungicide, the number of viable of each microbial inoculum is:
6. according to the aquaculture complex micro organism fungicide described in claim 1-5, it is characterized in that, in above-mentioned complex micro organism fungicide, add auxiliary material to be prepared into pulvis, tablet, granula and capsule.
7. the aquaculture complex micro organism fungicide described in claim 1-6 is for improveing the application of water quality and/or control aquatic living things disease.
8. purposes according to claim 7, is characterized in that, described aquaculture complex micro organism fungicide is for improving the application of water body color, increase water transparency, reduction ammonia-nitrogen content and COD.
9. purposes according to claim 7, is characterized in that, described aquaculture complex micro organism fungicide is for reducing the application of disease generation in catfish cultivation.
10. purposes according to claim 9, is characterized in that, described disease is catfish putrid skin disease, catfish white skin disease, catfish black matrix disease.
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| CN104450576A (en) * | 2014-12-10 | 2015-03-25 | 苏州埃瑞特生物技术有限公司 | Liquid preparation of bacillus pumilus and bdellovibrio and application thereof |
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| CN104894007A (en) * | 2015-05-12 | 2015-09-09 | 安徽农业大学 | Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses |
| CN105731651A (en) * | 2014-12-11 | 2016-07-06 | 领绿生物镇江有限公司 | Microbial preparation with multiple effects in purifying water, health care and inhibiting Vibrio and preparation method thereof |
| CN105907690A (en) * | 2016-07-06 | 2016-08-31 | 苏州市相城区阳澄湖镇剑美水产生态养殖专业合作社 | Microbial preparation for aquiculture |
| CN107555607A (en) * | 2017-08-21 | 2018-01-09 | 吴杨 | A kind of compound oxygenation agent of biology and preparation method thereof |
| CN109775866A (en) * | 2019-03-13 | 2019-05-21 | 南京工业大学 | Preparation method of composite sustained-release microcapsule preparation for treating ammonia nitrogen in aquaculture water |
| CN111939180A (en) * | 2019-05-17 | 2020-11-17 | 上海绿奥生物科技有限公司 | Compound preparation for treating enteritis of turbot and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104431521A (en) * | 2014-12-10 | 2015-03-25 | 苏州埃瑞特生物技术有限公司 | Preparation method of powdery preparation of bacillus pumilus and bdellovibrio |
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| CN105731651A (en) * | 2014-12-11 | 2016-07-06 | 领绿生物镇江有限公司 | Microbial preparation with multiple effects in purifying water, health care and inhibiting Vibrio and preparation method thereof |
| CN104593352A (en) * | 2015-01-08 | 2015-05-06 | 国家海洋局第三海洋研究所 | Compound microorganism microbial agent for promoting intestinal health of aquatic animals and preparation method of microbial agent |
| CN104894007A (en) * | 2015-05-12 | 2015-09-09 | 安徽农业大学 | Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses |
| CN104894007B (en) * | 2015-05-12 | 2019-07-05 | 安徽全民环保科技有限公司 | Compound microbial preparation, preparation method and application in treatment of animal carcasses |
| CN105907690A (en) * | 2016-07-06 | 2016-08-31 | 苏州市相城区阳澄湖镇剑美水产生态养殖专业合作社 | Microbial preparation for aquiculture |
| CN107555607A (en) * | 2017-08-21 | 2018-01-09 | 吴杨 | A kind of compound oxygenation agent of biology and preparation method thereof |
| CN107555607B (en) * | 2017-08-21 | 2020-10-27 | 吴杨 | Biological composite oxygen increasing agent and preparation method thereof |
| CN109775866A (en) * | 2019-03-13 | 2019-05-21 | 南京工业大学 | Preparation method of composite sustained-release microcapsule preparation for treating ammonia nitrogen in aquaculture water |
| CN111939180A (en) * | 2019-05-17 | 2020-11-17 | 上海绿奥生物科技有限公司 | Compound preparation for treating enteritis of turbot and preparation method thereof |
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