CN103601302A - Compound bacillus subtilis sustained-release tablet - Google Patents
Compound bacillus subtilis sustained-release tablet Download PDFInfo
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Abstract
Relating to the technical field of water environment improvement and repair in aquaculture, the invention provides a compound bacillus subtilis sustained-release tablet, which comprises, by weight: 2-9% of bacillus subtilis raw powder, 73-96% of sodium percarbonate, 1-10% of a sustained release agent, and 1-8% of a stabilizer. The invention aims to provide the compound bacillus subtilis sustained-release tablet. The product has a significant improvement effect on the culture water environment, is convenient to use, and avoids the problem of culture water oxygen deficiency.
Description
Technical field
The present invention relates to improvement and the recovery technique field of aquaculture water surrounding, be specifically related to a kind of compound subtilis slow releasing tablet.
Background technology
Along with developing rapidly of culture fishery, the improving constantly of cultivation density, in water, residual bait, ight soil, animals and plants corpse are constantly accumulated, and cause aquaculture water to pollute more and more serious.In aquaculture water, organic loading is excessive, can cause water quality deterioration, and cause cultivated animals disease grow, even dead.Therefore, the pollution of cultivation water environment has become with deterioration the principal element that restriction culture fishery develops, also restricts the development of China's fishery economic simultaneously.Especially at the current healthy aquaculture of wideling popularize, while producing nuisanceless fishery products, to the pattern of preventing and treating of aquatic animal disease, by the pharmacological agent from traditional, be also that main turning to be take the comprehensive regulation of cultivation water environment and reparation as main, therefore, the improvement of cultivation water environment and recovery technique have important effect in aquaculture.
In aquaculture industry, mainly by the method for physics, chemistry, biological-ecological, cultivation water environment is improved and repaired at present, and derive thus Series of Water quality improving agent product.Chemical restoration is by chemical reaction, to be eliminated or reduced a kind of method of pollutant in water, although the effect of chemical classes product rapidly, but lacks long-lasting, and some products also can form secondary pollution, and some use costs are higher.
Biological restoration utilizes specific biology to eliminate or enrichment environment pollutent exactly under certain condition, thereby plays the reparation of polluted water environment and improving effect.What in aquaculture, extensively adopt at present is microorganism repairing method, and the main bacterial classification using has: light and bacterium, genus bacillus, nitrobacteria, lactobacillus, yeast and some complex microorganism preparations etc.Wherein with bacillus preparation because its degraded is organic, improvement eutrophication water effect stability is definite, and shelf life of products is long and use the extensivelyst, and apply maximum in genus bacillus, is subtilis.But subtilis is a kind of aerobic microbiological, in use procedure, can consume dissolved oxygen in water, improper use or the excessive water hypoxia that easily causes, even cause the death of cultivated animals.Bacillus subtilis formulation product in industry generally is liquid preparation or solid powder now, when reality is used, be all to put in aquaculture water according to formulation products specification sheets consumption is disposable, subtilis enters after water body, because of its generation cycle short, rate of propagation is fast, this easily causes the sudden anoxic of aquaculture water, thereby causes the death of cultivated animals; In addition, liquid or solid bacillus subtilis formulation product generally need to activate 1～2 hour before use, and this has brought inconvenience while also using to user.
Summary of the invention
For the defect existing in prior art, the object of the present invention is to provide a kind of compound subtilis slow releasing tablet, its improved effect to cultivation water environment is remarkable, easy-to-use, and avoids aquaculture water anoxic problem.
For reaching above object, the technical scheme that the present invention takes is: a kind of compound subtilis slow releasing tablet, by weight percentage, its component comprises: the former powder 2～9% of subtilis, SPC-D 73～96%, sustained release dosage 1～10%, stablizer 1～8%.
On the basis of technique scheme, by weight percentage, its component comprises: the former powder 3～8% of subtilis, SPC-D 78～93%, sustained release dosage 3～8%, stablizer 1～6%.
On the basis of technique scheme, by weight percentage, its component comprises: the former powder 4～7% of subtilis, SPC-D 83～90%, sustained release dosage 4～6%, stablizer 2～4%.
On the basis of technique scheme, by weight percentage, its component comprises: the former powder 6% of subtilis, SPC-D 86%, sustained release dosage 5%, stablizer 3%.
On the basis of technique scheme, the former powder of described subtilis is glucose embedding.
On the basis of technique scheme, described sustained release dosage is one or more the mixture in sodium alginate, HPMC, ethyl cellulose, polyvinyl alcohol.
On the basis of technique scheme, described sustained release dosage is sodium alginate.
On the basis of technique scheme, described stablizer is one or more the mixture in diatomite, disodium ethylene diamine tetraacetate, white carbon black, polyvinylpolypyrrolidone, methylcellulose gum.
On the basis of technique scheme, described stablizer is disodium ethylene diamine tetraacetate.
Beneficial effect of the present invention is:
1, the component of slow releasing tablet of the present invention comprises SPC-D, not only can fast activating subtilis, promote the growth and reproduction of subtilis, and can increase the dissolved oxygen in cultivation water environment, solved the water hypoxia causing because of subtilis amount reproduction oxygen consumption.
2, slow releasing tablet of the present invention is a kind of microbial preparation, is environment-friendly high-efficiency, noresidue, avirulent green aquifer amendment.
3, the slow releasing tablet of product of the present invention for adopting compressing dry granulation technology to be prepared from, direct dry spilling during use, without the activation of oxygenation in advance, easy to use.After it enters water body, can maintain 16～24 hours time of releasing, avoided liquid or pulvis producing bacillus subtilis product enter after water body because of subtilis simultaneously amount reproduction cause the sudden anoxic of aquaculture water.
Embodiment
A compound subtilis slow releasing tablet, by weight percentage, its component comprises: the former powder 2～9% of subtilis, SPC-D 73～96%, sustained release dosage 1～10%, stablizer 1～8%.Preferred ingredient comprises: the former powder 3～8% of subtilis, SPC-D 78～93%, sustained release dosage 3～8%, stablizer 1～6%.Further preferred ingredient comprises: the former powder 4～7% of subtilis, SPC-D 83～90%, sustained release dosage 4～6%, stablizer 2～4%.Most preferred composition comprises: the former powder 6% of subtilis, SPC-D 86%, sustained release dosage 5%, stablizer 3%.
Described sustained release dosage is one or more the mixture in sodium alginate, HPMC (HPMCK4M), ethyl cellulose, polyvinyl alcohol, and preferred sustained release dosage is sodium alginate.The mixture of one or more in diatomite, disodium ethylene diamine tetraacetate, white carbon black, polyvinylpolypyrrolidone, methylcellulose gum of described stablizer, preferred stablizer is disodium ethylene diamine tetraacetate.
Subtilis in the former powder of described subtilis is a kind of rod-short, without pod membrane, peritrichous, the gram positive bacterium that can move, it is extensively distributed in the organism of soil and corruption, easily withered grass, soak in juice and breed, available protein, multiple sugar and amylolysis tryptophane form indoles.Subtilis can consume the organic matter in water body in a large number, is translated into small molecular organic acid, ammonia etc., therefore can effectively reduce organic content in water after using, accelerates decomposition, the conversion of residual bait, ight soil etc. in culturing pool; Meanwhile, improve harmful blue-green algae and spread unchecked the water turbidity problem causing, there is very strong Function For Purifying Water.But subtilis is the chmosynthetic heterotrophs microorganism of strict aerobic growth, and improper use or excessive meeting cause water hypoxia.The former powder of described subtilis is glucose embedding.
Described SPC-D can effectively increase Dissolved Oxygen in Water in aquaculture water management; The advantage of SPC-D is the stability of storage and with the good compatibleness of other composition etc., aspect commercial use, conventionally with materials such as vitriol and silicate, wrapped up, the SPC-D that obtains dressing improves it and joins middle side for the requirement of storage stability at Industrial products.
The preparation method of described compound subtilis slow releasing tablet, comprises the following steps::
S1. mix: in weight ratio, take 73～96% SPC-D, the former powder of 2～9% subtilis, 1～10% sustained release dosage, 1～8% stablizer and mix by equivalent incremental method, obtain always mixed thing;
S2. compressing tablet: the total mixed thing mixing is carried out to compressing dry granulation on dry powder sheeting machine, make compound subtilis slow releasing tablet.
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
A compound subtilis slow releasing tablet, its component comprises: the former powder 9kg of subtilis, SPC-D 74kg, sodium alginate 9kg, diatomite 8kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 9kg of subtilis, SPC-D 26kg, sodium alginate 9kg and diatomite 8kg and carry out 5～10 minutes premixs, obtain pre-composition, then take SPC-D 48kg and pre-composition carry out 10～20 minutes total mixed, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Embodiment 2:
A compound subtilis slow releasing tablet, its component comprises: the former powder 8kg of subtilis, SPC-D 77kg, HPMC 8kg, disodium ethylene diamine tetraacetate 7kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 8kg of subtilis, SPC-D 23kg, HPMC 8kg, disodium ethylene diamine tetraacetate 7kg and carry out 5～10 minutes premixs, obtain pre-composition, take respectively again SPC-D 46kg, 8kg, add successively the pre-composition in mixing, carry out 10～20 minutes total mixing, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Embodiment 3
A compound subtilis slow releasing tablet, its component comprises: the former powder 6kg of subtilis, SPC-D 83kg, HPMC 6kg, diatomite 5kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 6kg of subtilis, SPC-D 17kg, HPMC 6kg, diatomite 5kg and carry out 5～10 minutes premixs, obtain pre-composition, then take respectively SPC-D 34kg, 32kg, add successively the pre-composition in mixing, carry out 10～20 minutes total mixing, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Embodiment 4
A compound subtilis slow releasing tablet, its component comprises: the former powder 5kg of subtilis, SPC-D 86kg, sodium alginate 5kg, methylcellulose gum 4kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 5kg of subtilis, SPC-D 14kg, sodium alginate 5kg, methylcellulose gum 4kg and carry out 5～10 minutes premixs, obtain pre-composition, then take respectively SPC-D 28kg, 44kg, add successively the pre-composition in mixing, carry out 10～20 minutes total mixing, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Embodiment 5
A compound subtilis slow releasing tablet, its component comprises: the former powder 4kg of subtilis, SPC-D 89kg, ethyl cellulose 4kg, polyvinylpolypyrrolidone 3kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 4kg of subtilis, SPC-D 11kg, ethyl cellulose 4kg, polyvinylpolypyrrolidone 3kg and carry out 5～10 minutes premixs, obtain pre-composition, take respectively again SPC-D 22kg, 44kg, 12kg, add successively the pre-composition in mixing, carry out 10～20 minutes total mixing, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Embodiment 6
A compound subtilis slow releasing tablet, its component comprises: the former powder 3kg of subtilis, SPC-D 92kg, polyvinyl alcohol 3kg, methylcellulose gum 2kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 3kg of subtilis, SPC-D 8kg, polyvinyl alcohol 3kg, methylcellulose gum 2kg and carry out 5～10 minutes premixs, obtain pre-composition, take respectively again SPC-D 16kg, 32kg, 36kg, add successively the pre-composition in mixing, carry out 10～20 minutes total mixing, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Embodiment 7
A compound subtilis slow releasing tablet, its component comprises: the former powder 2kg of subtilis, SPC-D 95kg, ethyl cellulose 2kg, methylcellulose gum 1kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 2kg of subtilis, SPC-D 5kg, ethyl cellulose 2kg, methylcellulose gum 1kg and carry out 5～10 minutes premixs, obtain pre-composition, take respectively again SPC-D 10kg, 20kg, 40kg, 20kg, add successively the pre-composition in mixing, carry out 10～20 minutes total mixing, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Embodiment 8
A compound subtilis slow releasing tablet, its component comprises: the former powder 6kg of subtilis, SPC-D 86kg, sodium alginate 5kg, disodium ethylene diamine tetraacetate 3kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 6kg of subtilis, SPC-D 14kg, sodium alginate 5kg, disodium ethylene diamine tetraacetate 3kg carry out 5～10 minutes premixs, obtain pre-composition, take respectively again SPC-D 28kg, 44kg, add successively the pre-composition in mixing, carry out 10～20 minutes total mixing, obtain always mixed thing;
S2. will always mix thing and carry out compressing dry granulation with dry powder sheeting machine, every heavy 1 gram, makes described compound subtilis slow releasing tablet.
Requirement and technological method according to the < < of Ministry of Agriculture fishing medicine clinical trial technical specifications > >, carry out safety testing and pharmacodynamics test, and carry out statistical study.
1, safety testing: selected the at present main cultured fishes such as grass carp, black carp, silver carp, flathead, crucian, Megalobrama amblycephala, bream, carp, dace, tilapia, mandarin fish, perch, Yellow catfish, Channel-catfish fish as tested fish, carried out general toxicity proof test.In water body, drop into the product that embodiment 8 makes, in water body, the concentration of this product is when 20ppm, and tested fish activity is normal, and not occurring stress phenomenon (as twitched, swim etc.), without the phenomenon generation of being poisoned to death.
2, large-scale farming pond effect assessment:
Product of the present invention successively in Hubei, the whole nation such as Hunan, Guangdong, Guangxi, Zhejiang, Jiangsu, Henan, northeast mainly culturing area carried out large-scale farming pond application test, strive all fish species in safety testing and other raised together with to fish.The product that embodiment 8 is made carries out dosing by 3 times of actual amount, change, and observation breed variety has or not anoxic floating head phenomenon to carry out effect assessment according to the dissolved oxygen of cultivating pool after medication.By the observation of more than 60 routine cultivating pools is added up, double to use after most preferred composition product of the present invention, pond dissolved oxygen was slow decreasing trend in initial 28 hours, but all not lower than the required dissolved oxygen threshold values of cultured fishes, after 28 hours, dissolved oxygen starts to rise, and tend towards stability gradually, the cultured fishes activity in all test cultivation fish pond is normal, does not all occur anoxia phenomenon.
The present invention is not limited to above-mentioned embodiment, for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvements and modifications, within these improvements and modifications are also considered as protection scope of the present invention.The content not being described in detail in this specification sheets belongs to the known prior art of professional and technical personnel in the field.
Claims (9)
1. a compound subtilis slow releasing tablet, is characterized in that, by weight percentage, its component comprises: the former powder 2～9% of subtilis, SPC-D 73～96%, sustained release dosage 1～10%, stablizer 1～8%.
2. compound subtilis slow releasing tablet as claimed in claim 1, is characterized in that, by weight percentage, its component comprises: the former powder 3～8% of subtilis, SPC-D 78～93%, sustained release dosage 3～8%, stablizer 1～6%.
3. compound subtilis slow releasing tablet as claimed in claim 2, is characterized in that, by weight percentage, its component comprises: the former powder 4～7% of subtilis, SPC-D 83～90%, sustained release dosage 4～6%, stablizer 2～4%.
4. the compound subtilis slow releasing tablet as described in claim 1,2 or 3, is characterized in that, by weight percentage, its component comprises: the former powder 6% of subtilis, SPC-D 86%, sustained release dosage 5%, stablizer 3%.
5. compound subtilis slow releasing tablet as claimed in claim 1, is characterized in that: the former powder of described subtilis is glucose embedding.
6. compound subtilis slow releasing tablet as claimed in claim 1, is characterized in that: described sustained release dosage is one or more the mixture in sodium alginate, HPMC, ethyl cellulose, polyvinyl alcohol.
7. compound subtilis slow releasing tablet as claimed in claim 6, is characterized in that: described sustained release dosage is sodium alginate.
8. compound subtilis slow releasing tablet as claimed in claim 1, is characterized in that: described stablizer is one or more the mixture in diatomite, disodium ethylene diamine tetraacetate, white carbon black, polyvinylpolypyrrolidone, methylcellulose gum.
9. compound subtilis slow releasing tablet as claimed in claim 8, is characterized in that: described stablizer is disodium ethylene diamine tetraacetate.
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| CN108651454A (en) * | 2018-04-09 | 2018-10-16 | 陕西科技大学 | A kind of preparation method of bio-microcapsule |
| CN111732203A (en) * | 2020-06-09 | 2020-10-02 | 上海中汇水生态科技有限公司 | Artificial aquatic plant water purifying device |
| CN115261267A (en) * | 2022-07-18 | 2022-11-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Application of brown algae oligosaccharide in promoting movement of bacillus and field planting of plant rhizosphere |
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