CN103601302B - A kind of compound subtilis slow releasing tablet - Google Patents
A kind of compound subtilis slow releasing tablet Download PDFInfo
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- CN103601302B CN103601302B CN201310651264.9A CN201310651264A CN103601302B CN 103601302 B CN103601302 B CN 103601302B CN 201310651264 A CN201310651264 A CN 201310651264A CN 103601302 B CN103601302 B CN 103601302B
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Abstract
A kind of compound subtilis slow releasing tablet, relate to improvement and the recovery technique field of aquaculture water surrounding, the product in the present invention is by weight percentage, component comprises the former powder 2 ~ 9% of subtilis, SPC-D 73 ~ 96%, sustained release dosage 1 ~ 10%, stablizer 1 ~ 8%.The object of the present invention is to provide a kind of compound subtilis slow releasing tablet, the improved effect of this product to cultivation water environment is remarkable, easy-to-use, and avoids aquaculture water anoxic problem.
Description
Technical field
The present invention relates to improvement and the recovery technique field of aquaculture water surrounding, be specifically related to a kind of compound subtilis slow releasing tablet.
Background technology
Along with developing rapidly of culture fishery, improving constantly of cultivation density, in water, residual bait, ight soil, animals and plants corpse are constantly accumulated, and cause aquaculture water to pollute more and more serious.In aquaculture water, organic loading is excessive, can cause water quality deterioration, and cause cultivated animals disease to be grown, even dead.Therefore, the pollution of cultivation water environment has become with deterioration the principal element restricting culture fishery and develop, also restricts the development of China's fishery economic simultaneously.Especially healthy aquaculture is widelyd popularize current, when producing nuisanceless fishery products, to the control models of aquatic animal disease also will be from traditional pharmacological agent main turn to based on the comprehensive regulation of cultivation water environment with repair, therefore, the improvement of cultivation water environment and recovery technique have important effect in aquaculture.
At present carry out improve to cultivation water environment mainly through the method for physics, chemistry, biological-ecological in aquaculture industry and repair, and deriving Series of Water quality improving agent product thus.Chemical restoration is a kind of method being eliminated or reduce pollutant in water by chemical reaction, although the effect of chemical classes product rapidly, but lack long-lasting, some products also can form secondary pollution, and some use costs are higher.
Biological restoration utilizes specific biology to carry out eliminating or enrichment environment pollutent exactly under certain condition, thus the reparation played polluted water environment and improving effect.What extensively adopt in aquaculture at present is microorganism remediation method, and the main bacterial classification used has: light and bacterium, genus bacillus, nitrobacteria, lactobacillus, yeast and some complex microorganism preparations etc.Wherein with bacillus preparation because its degraded is organic, improvement eutrophication water effect stability is definite, and shelf life of products is long and use the widest, and to apply maximum in genus bacillus be subtilis.But subtilis is a kind of aerobic microbiological, in use procedure, can dissolved oxygen in water be consumed, improper use or excessively easily cause water hypoxia, even cause the death of cultivated animals.Bacillus subtilis formulation product now in industry generally is liquid preparation or solid powder, all put in aquaculture water according to formulation products specification sheets consumption is disposable when reality uses, after subtilis enters water body, because its generation cycle is short, rate of propagation is fast, this easily causes the sudden anoxic of aquaculture water, thus causes the death of cultivated animals; In addition, liquid or solid bacillus subtilis formulation product generally needs activation 1 ~ 2 hour before use, brings inconvenience when this also uses to user.
Summary of the invention
For the defect existed in prior art, the object of the present invention is to provide a kind of compound subtilis slow releasing tablet, it is remarkable, easy-to-use to the improved effect of cultivation water environment, and avoids aquaculture water anoxic problem.
For reaching above object, the technical scheme that the present invention takes is: a kind of compound subtilis slow releasing tablet, and by weight percentage, its component comprises: the former powder 2 ~ 9% of subtilis, SPC-D 73 ~ 96%, sustained release dosage 1 ~ 10%, stablizer 1 ~ 8%.
On the basis of technique scheme, by weight percentage, its component comprises: the former powder 3 ~ 8% of subtilis, SPC-D 78 ~ 93%, sustained release dosage 3 ~ 8%, stablizer 1 ~ 6%.
On the basis of technique scheme, by weight percentage, its component comprises: the former powder 4 ~ 7% of subtilis, SPC-D 83 ~ 90%, sustained release dosage 4 ~ 6%, stablizer 2 ~ 4%.
On the basis of technique scheme, by weight percentage, its component comprises: the former powder 6% of subtilis, SPC-D 86%, sustained release dosage 5%, stablizer 3%.
On the basis of technique scheme, the former powder of described subtilis is glucose embedding.
On the basis of technique scheme, described sustained release dosage is the mixture of one or more in sodium alginate, HPMC, ethyl cellulose, polyvinyl alcohol.
On the basis of technique scheme, described sustained release dosage is sodium alginate.
On the basis of technique scheme, described stablizer is the mixture of one or more in diatomite, disodium ethylene diamine tetraacetate, white carbon black, polyvinylpolypyrrolidone, methylcellulose gum.
On the basis of technique scheme, described stablizer is disodium ethylene diamine tetraacetate.
Beneficial effect of the present invention is:
1, the component of slow releasing tablet of the present invention comprises SPC-D, not only can fast activating subtilis, promote the growth and reproduction of subtilis, and the dissolved oxygen in cultivation water environment can be increased, solve the water hypoxia caused because of subtilis amount reproduction oxygen consumption.
2, slow releasing tablet of the present invention is a kind of microbial preparation, is environment-friendly high-efficiency, noresidue, avirulent green aquifer amendment.
3, product of the present invention is the slow releasing tablet adopting compressing dry granulation technology to be prepared from, and directly dryly during use spills, without the need to the activation of oxygenation in advance, easy to use.After it enters water body, time of releasing can maintain 16 ~ 24 hours, avoid liquid or pulvis producing bacillus subtilis product enter water body after because of subtilis amount reproduction and cause the sudden anoxic of aquaculture water simultaneously.
Embodiment
A kind of compound subtilis slow releasing tablet, by weight percentage, its component comprises: the former powder 2 ~ 9% of subtilis, SPC-D 73 ~ 96%, sustained release dosage 1 ~ 10%, stablizer 1 ~ 8%.Preferred ingredient comprises: the former powder 3 ~ 8% of subtilis, SPC-D 78 ~ 93%, sustained release dosage 3 ~ 8%, stablizer 1 ~ 6%.Further preferred ingredient comprises: the former powder 4 ~ 7% of subtilis, SPC-D 83 ~ 90%, sustained release dosage 4 ~ 6%, stablizer 2 ~ 4%.Most preferred composition comprises: the former powder 6% of subtilis, SPC-D 86%, sustained release dosage 5%, stablizer 3%.
Described sustained release dosage is the mixture of one or more in sodium alginate, HPMC (HPMCK4M), ethyl cellulose, polyvinyl alcohol, and preferred sustained release dosage is sodium alginate.Described stablizer is by the mixture of one or more in diatomite, disodium ethylene diamine tetraacetate, white carbon black, polyvinylpolypyrrolidone, methylcellulose gum, and preferred stablizer is disodium ethylene diamine tetraacetate.
Subtilis in the former powder of described subtilis is a kind of rod-short, without pod membrane, peritrichous, the gram positive bacterium that can move, it is extensively distributed in the organism of soil and corruption, easily breed in withered grass leaching juice, available protein, multiple sugar and amylolysis tryptophane form indoles.Subtilis can consume the organic matter in water body in a large number, is translated into small molecular organic acid, ammonia etc., therefore can effectively reduce organic content in water after using, and accelerates the decomposition of residual bait, ight soil etc. in culturing pool, conversion; Meanwhile, improve harmful blue-green algae and spread unchecked the water turbidity problem caused, there is very strong Function For Purifying Water.But subtilis is the chmosynthetic heterotrophs microorganism of strict aerobic growth, and improper use or excessive meeting cause water hypoxia.The former powder of described subtilis is glucose embedding.
Described SPC-D effectively can increase Dissolved Oxygen in Water in aquaculture water management; The advantage of SPC-D is the stability of storage and the compatibleness etc. good with other composition, in commercial use, the materials such as usual vitriol and silicate are wrapped up, and obtain the SPC-D of dressing to improve its square requirement for storage stability in Industrial products are joined.
The preparation method of described compound subtilis slow releasing tablet, comprises the following steps::
S1. mix: with mass ratio range, take 73 ~ 96% SPC-D, the former powder of 2 ~ 9% subtilis, 1 ~ 10% sustained release dosage, 1 ~ 8% stablizer mix by equal increments method, obtain always mixed thing;
S2. compressing tablet: the total mixed thing mixed is carried out compressing dry granulation on dry powder sheeting machine, obtained compound subtilis slow releasing tablet.
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 9kg of subtilis, SPC-D 74kg, sodium alginate 9kg, diatomite 8kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 9kg of subtilis, SPC-D 26kg, sodium alginate 9kg and diatomite 8kg and carry out 5 ~ 10 minutes premixs, obtain pre-composition, then take SPC-D 48kg and pre-composition carry out 10 ~ 20 minutes always mixed, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
Embodiment 2:
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 8kg of subtilis, SPC-D 77kg, HPMC 8kg, disodium ethylene diamine tetraacetate 7kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 8kg of subtilis, SPC-D 23kg, HPMC 8kg, disodium ethylene diamine tetraacetate 7kg carry out 5 ~ 10 minutes premixs, obtain pre-composition, take SPC-D 46kg, 8kg respectively again, add the pre-composition in mixing successively, carry out always mixing for 10 ~ 20 minutes, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
Embodiment 3
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 6kg of subtilis, SPC-D 83kg, HPMC 6kg, diatomite 5kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 6kg of subtilis, SPC-D 17kg, HPMC 6kg, diatomite 5kg carry out 5 ~ 10 minutes premixs, obtain pre-composition, then take SPC-D 34kg, 32kg respectively, add the pre-composition in mixing successively, carry out always mixing for 10 ~ 20 minutes, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
Embodiment 4
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 5kg of subtilis, SPC-D 86kg, sodium alginate 5kg, methylcellulose gum 4kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 5kg of subtilis, SPC-D 14kg, sodium alginate 5kg, methylcellulose gum 4kg carry out 5 ~ 10 minutes premixs, obtain pre-composition, then take SPC-D 28kg, 44kg respectively, add the pre-composition in mixing successively, carry out always mixing for 10 ~ 20 minutes, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
Embodiment 5
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 4kg of subtilis, SPC-D 89kg, ethyl cellulose 4kg, polyvinylpolypyrrolidone 3kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 4kg of subtilis, SPC-D 11kg, ethyl cellulose 4kg, polyvinylpolypyrrolidone 3kg carry out 5 ~ 10 minutes premixs, obtain pre-composition, take SPC-D 22kg, 44kg, 12kg respectively again, add the pre-composition in mixing successively, carry out always mixing for 10 ~ 20 minutes, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
Embodiment 6
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 3kg of subtilis, SPC-D 92kg, polyvinyl alcohol 3kg, methylcellulose gum 2kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 3kg of subtilis, SPC-D 8kg, polyvinyl alcohol 3kg, methylcellulose gum 2kg carry out 5 ~ 10 minutes premixs, obtain pre-composition, take SPC-D 16kg, 32kg, 36kg respectively again, add the pre-composition in mixing successively, carry out always mixing for 10 ~ 20 minutes, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
Embodiment 7
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 2kg of subtilis, SPC-D 95kg, ethyl cellulose 2kg, methylcellulose gum 1kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 2kg of subtilis, SPC-D 5kg, ethyl cellulose 2kg, methylcellulose gum 1kg carry out 5 ~ 10 minutes premixs, obtain pre-composition, take SPC-D 10kg, 20kg, 40kg, 20kg respectively again, add the pre-composition in mixing successively, carry out always mixing for 10 ~ 20 minutes, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
Embodiment 8
A kind of compound subtilis slow releasing tablet, its component comprises: the former powder 6kg of subtilis, SPC-D 86kg, sodium alginate 5kg, disodium ethylene diamine tetraacetate 3kg.
The preparation method of described compound subtilis slow releasing tablet:
S1. take the former powder 6kg of subtilis, SPC-D 14kg, sodium alginate 5kg, disodium ethylene diamine tetraacetate 3kg carry out 5 ~ 10 minutes premixs, obtain pre-composition, take SPC-D 28kg, 44kg respectively again, add the pre-composition in mixing successively, carry out always mixing for 10 ~ 20 minutes, obtain always mixed thing;
S2. carry out compressing dry granulation by always mixing thing dry powder sheeting machine, every sheet weighs 1 gram, obtained described compound subtilis slow releasing tablet.
According to requirement and the technological method of Ministry of Agriculture's " fishing medicine clinical trial technical specifications ", carry out safety testing and pharmacodynamics test, and carry out statistical study.
1, safety testing: have selected the main cultured fishes at present such as grass carp, black carp, silver carp, flathead, crucian, Megalobrama amblycephala, bream, carp, dace, tilapia, mandarin fish, perch, Yellow catfish, Channel-catfish fish and, as tested fish, carry out general toxicity proof test.In water body, drop into the product that embodiment 8 is obtained, when the concentration of this product in water body is at 20ppm, tested fish activity is normal, does not have appearance stress phenomenon (as twitched, swimming), without phenomenon generation of being poisoned to death.
2, large-scale farming pond effect assessment:
Product of the present invention successively in Hubei, Hunan, Guangdong, Guangxi, Zhejiang, Jiangsu, Henan, the main culturing area in the whole nation such as northeast carried out large-scale farming pond application test, strive and fish raised together with to all fish species in safety testing and other.Product obtained for embodiment 8 is carried out dosing by 3 times of actual amount, and the dissolved oxygen according to cultivating pool after medication changes, and observation breed variety carries out effect assessment with or without anoxic floating head phenomenon.By adding up the observation of more than 60 routine cultivating pools, after doubling to use most preferred composition product of the present invention, pond dissolved oxygen was slow downtrending in initial 28 hours, but all not lower than dissolved oxygen threshold values needed for cultured fishes, after 28 hours, dissolved oxygen starts to rise, and tend towards stability gradually, the cultured fishes activity in all test cultivation fish ponds is normal, does not all occur anoxia phenomenon.
The present invention is not limited to above-mentioned embodiment, and for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications are also considered as within protection scope of the present invention.The content be not described in detail in this specification sheets belongs to the known prior art of professional and technical personnel in the field.
Claims (6)
1. a compound subtilis slow releasing tablet, it is characterized in that, by weight percentage, its component comprises: the former powder 2 ~ 9% of subtilis, SPC-D 73 ~ 96%, sustained release dosage 1 ~ 10%, stablizer 1 ~ 8%, the former powder of described subtilis is glucose embedding, described sustained release dosage is sodium alginate, HPMC, ethyl cellulose, the mixture of one or more in polyvinyl alcohol, described stablizer is diatomite, disodium ethylene diamine tetraacetate, white carbon black, polyvinylpolypyrrolidone, the mixture of one or more in methylcellulose gum.
2. compound subtilis slow releasing tablet as claimed in claim 1, it is characterized in that, by weight percentage, its component comprises: the former powder 3 ~ 8% of subtilis, SPC-D 78 ~ 93%, sustained release dosage 3 ~ 8%, stablizer 1 ~ 6%.
3. compound subtilis slow releasing tablet as claimed in claim 2, it is characterized in that, by weight percentage, its component comprises: the former powder 4 ~ 7% of subtilis, SPC-D 83 ~ 90%, sustained release dosage 4 ~ 6%, stablizer 2 ~ 4%.
4. the compound subtilis slow releasing tablet as described in claim 1,2 or 3, is characterized in that, by weight percentage, its component comprises: the former powder 6% of subtilis, SPC-D 86%, sustained release dosage 5%, stablizer 3%.
5. compound subtilis slow releasing tablet as claimed in claim 1, is characterized in that: described sustained release dosage is sodium alginate.
6. compound subtilis slow releasing tablet as claimed in claim 1, is characterized in that: described stablizer is disodium ethylene diamine tetraacetate.
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| CN108341440B (en) * | 2018-03-02 | 2021-02-23 | 北京利华消防工程有限公司 | Water treatment agent, preparation method and method for treating fire-fighting water by using water treatment agent |
| CN108651454A (en) * | 2018-04-09 | 2018-10-16 | 陕西科技大学 | A kind of preparation method of bio-microcapsule |
| CN111732203B (en) * | 2020-06-09 | 2022-07-15 | 上海中汇水生态科技有限公司 | Artificial aquatic plant water purifying device |
| CN115261267B (en) * | 2022-07-18 | 2023-08-11 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Application of brown alginate oligosaccharides in promoting bacillus movement and plant rhizosphere colonisation |
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| CN1850660A (en) * | 2006-05-17 | 2006-10-25 | 天津生机集团有限公司 | Improved composition of base of aquatic cultivation pool |
| CN101580281A (en) * | 2009-06-08 | 2009-11-18 | 无锡中顺生物技术有限公司 | Substrate modifier for aquiculture and preparation method |
| CN101830567A (en) * | 2010-04-29 | 2010-09-15 | 南京神克隆科技有限公司 | Method and biochemical treatment agent for preventing and controlling blue-green algae |
| CN102232364A (en) * | 2010-05-08 | 2011-11-09 | 张玉明 | Method for preparing substrate material of Chinese herbal slow-release injection for environmental friendly aquaculture by using natural zeolite |
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| JPH026897A (en) * | 1988-01-21 | 1990-01-11 | Nissei Food Kk | Method of cleaning water by cleaning agent formed by mixing three materials |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1850660A (en) * | 2006-05-17 | 2006-10-25 | 天津生机集团有限公司 | Improved composition of base of aquatic cultivation pool |
| CN101580281A (en) * | 2009-06-08 | 2009-11-18 | 无锡中顺生物技术有限公司 | Substrate modifier for aquiculture and preparation method |
| CN101830567A (en) * | 2010-04-29 | 2010-09-15 | 南京神克隆科技有限公司 | Method and biochemical treatment agent for preventing and controlling blue-green algae |
| CN102232364A (en) * | 2010-05-08 | 2011-11-09 | 张玉明 | Method for preparing substrate material of Chinese herbal slow-release injection for environmental friendly aquaculture by using natural zeolite |
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