CN101904949B - Plant extract liquor and preparation method thereof - Google Patents
Plant extract liquor and preparation method thereof Download PDFInfo
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- CN101904949B CN101904949B CN201010225992XA CN201010225992A CN101904949B CN 101904949 B CN101904949 B CN 101904949B CN 201010225992X A CN201010225992X A CN 201010225992XA CN 201010225992 A CN201010225992 A CN 201010225992A CN 101904949 B CN101904949 B CN 101904949B
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Abstract
The invention provides plant extract liquor and a preparation method thereof. The liquor is composite liquor obtained by performing different fermentation reactions and antagonist treatment on the extract liquor of a plurality of plants of purslane, eclipta, bidens bipinnata, alfalfa, mum, grape, spinach and the like and auxiliary raw materials of sugar cane juice, glutinous rice vinegar and activated water in a certain proportion. The plant extract liquor of the invention is the liquor having polysaccharide multi-type protein derivative and has balancing and optimizing functions on the nature and organisms.
Description
Technical field
The present invention relates to a kind of extract and preparation method thereof, be specifically related to a kind of liquid that comes by plant extraction and preparation method thereof.
Background technology
The Nature ecological balance damage comes from many-side, and for example: the livestock culture industry is to the pollution of environment; Life-time service chemical fertilizer, herbicide, pesticide etc. are to the destruction of soil; Agricultural wastes are dealt with the environmental pollution that is caused improperly; The excessive use medicine of aquaculture is to the pollution of water quality; The pollution of food chain causes organism the generation of various source of disease to occur, particularly to the puzzlement of human health.
Summary of the invention
In order to overcome the problems referred to above, the invention provides plant extract liquor and preparation method thereof, this plant extraction liquid contains polysaccharide body polymorphic type protein derivatives, and the use of this plant extraction liquid can be played balance and optimization function to the Nature and organism.
The preparation method of plant extraction liquid of the present invention comprises the steps:
(1) extraction plant liquid: with Herba Portulacae, Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and Herba Spinaciae (bright product) the herb section of chopping up or crush worn-out respectively, 1 kilogram (1/4 kilogram of dry product) becomes 5 kilograms of liquid, the person of frying in shallow oil time 4-7 hour with the 8-15 kilogram activated water decocting and concentrating of heating;
(2) plant concentrated extract fermentation reaction treatment: 5 kilograms of concentrated extracts of Herba Portulacae described in the step (1), Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and Herba Spinaciae are respectively charged in the ceramic pot, add auxiliary material, potting is closed 60-90 days then;
(3) antagonism stabilized treatment: the liquid after the Herba Portulacae in the step (2), Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and the Herba Spinaciae fermentation is removed precipitate and got liquid 2-3 kilogram, and every kind of liquid is done mutual antagonism stabilized treatment 60-90 days respectively; For example: Herba Portulacae and Herba Ecliptae, Herba Portulacae and Herba Bidentis Bipinnatae, Herba Ecliptae and Herba Bidentis Bipinnatae etc.
(4) centrifugal: liquid 1-2 kilogram on it is got for every group in the stable back of plant concentrated extract antagonism, and the reuse centrifugal method is repeatedly centrifugal, gets the about 0.5-1 kilogram of liquid mixed preparing on it for every group.
The contained composition of above-mentioned used plant is as follows:
Herba Portulacae has another name called Semen Benincasae dish (Portulaca oleracea L.), and herb contains a large amount of noradrenalins and volume potassium salt; Still contain alkaloid, Coumarins, flavonoid, cardiotonic glycoside and anthraquinone glycoside.
Herba Ecliptae has another name called Herba Ecliptae (Eclipta Prostrata L.), and herb contains Saponin, nicotine, tannin, vitamin A, Peng Qi chrysanthemum lactone, Qu Jia Ji Peng Qi chrysanthemum lactone.
Herba Bidentis Bipinnatae has another name called bidens (Bidens bipinnata L.), and herb contains alkaloid, tannin, Saponin, flavonoid glycoside; Stem and leaf contains volatile oil, tannin, amaroid, choline.
Herba Medicaginis has another name called alfalfa (Medicago satiua L.), and alfalfa contains Saponin, lucernol, 7-hydroxy-11,12-(methylenedioxy)coumestan, daidzein; Regulation and Blue Flowers of alfalfa and purple flower master contain delphinidin and malvidin.
Flos Chrysanthemi has another name called Flos Chrysanthemi (Chrysanthemum morifolium Ramat), and flower and stem contain volatile oil, and adenine, choline, stachydrine are arranged, and flower contains chrysanthemum glycoside, aminoacid, flavonoid and mcg vitamin again.
Fructus Vitis viniferae has another name called careless Herba Tubocapsici Anomali (Vitis uinifera L.), and Fructus Vitis viniferae contains glucose, fructose, a little sugar, xylose, tartaric acid, oxalic acid, citric acid, malic acid; Pericarpium Vitis viniferae contains cyanidin, peonidin, delphinidin, petunidin, malvidin; Seed oil content 9.58% contains Jiao's property catechol, gallo catechin, gallate again.
Herba Spinaciae has another name called spinach rib (Spinacia oleracea L.), Ye Hanxin, folic acid, aminoacid, phylloxanthin, carotene, patuletin, spinacetin; Root contains spinasaponin A and B.
Caulis Sacchari sinensis has another name called dried sugarcane (Saccharum sinensis Roxb), contains several amino acids in the sugarcane juice, and asparagine, aspartic acid, glutamic acid, serine, alanine, lysine are arranged.
Wherein, auxiliary material is as catalytic action.Auxiliary material is preferably Caulis Sacchari sinensis juice and Oryza glutinosa vinegar; Its consumption is respectively: Caulis Sacchari sinensis juice 20-100 gram, Oryza glutinosa vinegar 10-20 gram.Caulis Sacchari sinensis juice is meant the liquid that goes out with bright cane milling.Oryza glutinosa vinegar is meant the Oryza glutinosa vinegar that market is ready-made.Activated water is the activated water of handling through mineral.
The plant extraction liquid that obtains by method of the present invention can reach required active ingredient, and as alkaloid, flavonoid, Saponin, tannin etc. are a kind of composite fluids with polysaccharide body polymorphic type protein derivatives.
Because plant extraction liquid of the present invention mainly contains the polytype protein derivatives, therefore can suppress harmful bacterium, the minimizing cell combines with harmful substance, change physiological phenomenon in the fowl carcass, make its probiotics be increased (as bacillus bifidus etc.), harmful bacterium is suppressed (as escherichia coli), makes that finally microbiologic population changes in the intestinal of fowl poultry.Why the Excreta of fowl poultry can foul smelling be to carry out the biochemical reaction of stink owing to protein resolves into sulfide, ammonia, amido (Amine) and indoles (Indole) material.If drink plant extraction liquid of the present invention, be pressed because this plant extraction liquid can make probiotics increase and be harmful to the microorganism growth and decline phenomenon that bacterium reduces, so the Excreta of fowl poultry can frowziness to fowl poultry.Simultaneously, synthesizing of the adjustable enzyme of plant extraction liquid of the present invention, thereby,, remedy more weak reaction chain of the interior biochemical metabolism of animal body or more weak chain reaction by this chain reaction for the chain reaction of the intravital ferment generation of animal, the obstruction of bottleneck reaction is descended, can strengthen simultaneously the activation of cell, balance with keep cells physiological, have the function of persistence concurrently, if with energy medicine, be exactly the reaction of energy accumulation.
Therefore, the protective effect that this plant extract liquor can play large natural environment reaches the effect of organism balance optimizing: can be used for making an addition in the drinking water of fowl poultry after (1), the allotment, the Excreta that makes fowl poultry can frowziness, spray liquid pig house and channel environment after will allocating simultaneously are controlled, make microorganism be able to balance; (2), be used for handling agricultural wastes (straw) and fowl and animal excrement and make its complete fermentation, carbonization stable, do not produce ferment in second time, reach harmless treatment and generate fertilizer; (3), organic fertilizer can be used for improving the soil the activity of enhancing soil property; (4), that the liquid after the allotment can be used to remove the medicine of aquatic products is residual, and food chain is purified.Liquid after the allotment can produce a kind of health preserving water can have health-care effect to human body.
Description of drawings
Fig. 1 is the production technology sketch of embodiment 1 allotment;
Fig. 2 is the production procedure of embodiment 2 pig drinking waters;
Fig. 3 is embodiment 3 allotment flow charts;
Fig. 4 is the flow chart that embodiment 4 aquatic products purify.
The specific embodiment
For making the present invention easier to understand,, further set forth the present invention below in conjunction with specific embodiment.Should be understood that this this embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Need to prove, for the ease of understanding, being described below of used some term in following example:
Activated water: the water after generally will handling by hydrotreater is called activated water.Water clusters is more little, and activity is big more.Activated water among the present invention is preferably the activated water of handling through mineral.Common drinking water is in conjunction with macromole water, and the angle between the O-H key of water is about 105 °.During external influence, the bond angle between them changes easily, and whole hydrone is a polar molecule.Owing to the effect of hydrogen bond, not between hydrone with single H
2O exists, but can be combined into big water clusters each other, and general water constitutes micel with 36～37 hydrones and exists.This macromole bunch group, it is poor to participate in the interior biochemical reaction of body, and lipid metabolism is poor.Even this water is pure again, can not well be absorbed by human body and the intravital cell of organism and chromosome.Simultaneously, the water of this annular coherent structure is easy to contain poisonous and hazardous Organic substance, makes the healthy sustain damage of human body.Distance between the hydrone of activated water is widened, and intermolecular conjugation (representing bonded tightness degree) is low, and hydrone is active big, permeates in cell easily.Activated water is the small-micelle water of short chain, and its structure more near the water in the human body cell, is absorbed by cell easily, can quench one's thirst fast.The air capacity that activated water is dissolved in the water increases, and is rich in oxygen in the water, and tonic function is arranged; The dissolving power height can quicken to remove vivotoxin and unnecessary refuse, improves immunity, strengthens vitality; Content of mineral substances is moderate, and it is neutral that pH value is, and has avoided the harm of the too much very few and inclined to one side alkali of pH value meta-acid of mineral to human body; Deuterium content harmful in the water is few.
Antagonism is meant that different hormones are to a certain physiological effect performance opposite effect.When being each factor synergy, a kind of factor can suppress or influence the another kind of factor and work.
Embodiment 1: a preferred embodiment of the preparation method of plant extraction liquid of the present invention
(1) extraction plant liquid: with Herba Portulacae, Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis and Herba Spinaciae (bright product) the herb section of chopping up respectively, Fructus Vitis viniferae crushing junk, Flos Chrysanthemi is got head inflorescence, these raw materials are got 1 kilogram of (if dry product respectively, then get 1/4 kilogram), the activated water of handling through mineral with the 8-15 kilogram decocting and concentrating of heating becomes 5 kilograms of liquid, decocting time 4-7 hour respectively;
(2) plant concentrated extract fermentation reaction treatment: 5 kilograms of concentrated extracts of Herba Portulacae described in the step (1), Herba Ecliptae, the outer grass of ghost, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and Herba Spinaciae are respectively charged in the ceramic pot, add Caulis Sacchari sinensis juice 20-100 gram, Oryza glutinosa vinegar 10-20 gram closed potting 60-90 days then as auxiliary material; Wherein, Caulis Sacchari sinensis juice is meant the liquid that goes out with bright cane milling, and Oryza glutinosa vinegar is meant the Oryza glutinosa vinegar that market is ready-made.
(3) antagonism stabilized treatment: the liquid after the Herba Portulacae in the step (2), Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and the Herba Spinaciae fermentation is removed precipitate and got liquid 2-3 kilogram, and every kind of liquid is done mutual antagonism stabilized treatment 60-90 days respectively; For example: Herba Portulacae and Herba Ecliptae, Herba Portulacae and Herba Bidentis Bipinnatae, Herba Ecliptae and Herba Bidentis Bipinnatae etc.
(4) centrifugal: liquid 1-2 kilogram on it is got for every group in the stable back of plant concentrated extract antagonism, and the reuse centrifugal method is repeatedly centrifugal, gets the about 0.5-1 kilogram of liquid mixed preparing on it for every group.
The liquid that above-mentioned mixed preparing obtains is plant extraction liquid of the present invention.
The mineral water treatment facility generates activated water, and plant extraction liquid is carrier with the activated water, and according to different uses, adjustable various composite fluid, Fig. 1 are the production technology sketches of allotment.As shown in Figure 1, the water source by two-pass reverse osmosis, mineral water treatment facility, enters the quantifying feed machine again through clean water treatment, adds plant extraction liquid and mix in the quantifying feed machine, enters the mineral water treatment facility again, becomes composite fluid.
This composite fluid finished product can have:
Be used for the composite fluid that pig is drunk;
Be used to handle the composite fluid of agricultural wastes (straw) and fowl and animal excrement;
The composite fluid that is used for the residual removal of aquatic products medicine;
Be used for human body is had the composite fluid (its allotment ratio 1: 1000 or 1: 2000 times) of health care.
Embodiment 2: the plant extraction composite fluid that obtains among the embodiment 1 is allocated in pig drinking water only.
The composite fluid that pig is drunk, its purpose make pig improve the forage protein absorbance, lower the excretal odor, with the activated water is carrier by (1: 500 times) allotment, quiet put 24 hours after, again by the mineral water treatment facility, further quantize allotment, its ratio (1: 500 times).Fig. 2 is the production procedure of pig drinking water: (being 1: 250000 times of whole process allotment ratio).As shown in Figure 2, the water source enters the mineral water treatment facility through the immersible pump motor, use quiet composite fluid solenoid control (1: 500) of putting after 24 hours simultaneously, make it to become the composite fluid (1: 250000) of activated water allotment, enter the immersible pump motor once more, and then, pass through tubing pump at last through the mineral water treatment facility, enter pig house by pig drinking water water pot, drink for pig.After the pig drink, following discovery is arranged:
(1) pig only drink its Excreta of back can frowziness, the stench on whole pig farm is controlled.By monitoring, reach Guangdong Province's provincial standard " Air Pollutant Emission limit value " (DB44/27-2001) in the secondary standard of second period.Illustrate that the plant extraction composite fluid that obtains among the embodiment 1 is applied to livestock breeding industry the protective effect of playing large natural environment is arranged.
(2) its meat was good after pig was only drunk, and content of beary metal is low, medicine is residual less, meet the non-polluted farm product standard-required fully.
(3) the detection through Guangzhou research of agricultural science institute victual comprehensive detection station to a pig drinking water, Carnis Sus domestica, operative norm NY5027-2001, NY5029-2001, detect as follows:
The pig drinking water detects (operative norm NY5027-2001)
The Carnis Sus domestica meat detects (operative norm NY5029-2001)
Embodiment 3: the plant extraction composite fluid allotment that obtains among the embodiment 1 is sprayed on agricultural wastes (straw) and the fowl and animal excrement produces fertilizer.
The plant extraction composite fluid that obtains among the embodiment 1 is allocated, with the activated water be carrier (1: 500) doubly, agricultural wastes (straw) and fowl and animal excrement are sprayed (straw must be pulverized), its complete fermentation is become thoroughly decomposed reaches the stable fertilizer of carbonization.Fig. 3 is the allotment flow chart, as shown in Figure 3, the water source enters the mineral water treatment facility through tubing pump, use the quiet composite fluid electromagnetic valve of putting after 24 hours to control (1: 500) simultaneously, make it to become the composite fluid (1: 250000) of activated water allotment, enter bucket, spray the garbage fowl and animal excrement by water pistol, handle by production procedure at last, obtain organic fertilizer.
(1) the plant extraction composite fluid that obtains among the embodiment 1 is passed through allotment, be sprayed on agricultural wastes (straw) and the fowl and animal excrement, be to ferment with low-temperature aerobic, the compost temperature of fertilizer is by the whole biochemical reaction in entering outward, the stench of fowl and animal excrement is controlled, can not produced secondary pollution and reach harmless treatment.
(2) the plant extraction composite fluid that obtains among the embodiment 1 is passed through allotment, be sprayed on agricultural wastes (straw) and the fowl and animal excrement, the fertilizer quality of its generation meets the NY525-2002 standard-required, detects (No.ZH20100001) survey report of inspecting by random samples through the supervision of Shaoguan mass metrology.As following table:
(3) will be applied to produce fertilizer after the plant extraction composite fluid allotment that obtain among the embodiment 1, its fertilizer can decompose toxin such as the pesticide that is piled up in soil for a long time and chemical fertilizer, can improve the soil, and strengthens the activity of soil property, increase fertility of land, crops can be increased production and improved productivity.Do the demonstration contrast test in rice field early by Nan County, Hunan bureau of agriculture clay fertilizer station.
1.1, test site and soil species
Test and Selection is carried out in Hydrargyri Oxydum Rubrum lake, Nan County town Wan Yuan village Zhou Yunliang and Chen Guanghua farmland covered by contract, is purple wattenshlick field for the examination soil species, and has a good transport service that soil fertility is even.Sample examination soil physical and chemical property before the test sees Table one.
Table one
1.2, for examination fertilizer: local habitual fertile, the Helianthi fertilizer
1.3, the test kind: early former rich
1.4, test handles: 1. contrast: local habits fertilization+local equivalent fertilizer; 2. handle: local habits fertilization+80 kilograms/667m of Helianthi fertilizer
2
Repetition is not established in test, and two families demonstration area is respectively 1.2 mu and 2.1 mu.Zhou Yunliang testing site site preparation and fertilization on April 13, sowing on April 14 was surveyed and is produced on July 9, harvesting on July 10.The Chen Guanghua testing site, April 17 site preparation and fertilization, April 18 sowing was surveyed and is produced on July 14, harvesting on July 15.
2, interpretation of result
2.1, the Helianthi fertilizer is to the influence (seeing Table two) of early rice fertility character
Table two is handled the influence to early rice fertility character
Table two shows: the Helianthi fertilizer is obvious with tillering stage and period of maturation to early rice fertility affect trait, and compares, and does sth. in advance 2-1 days tillering stage, and the period of maturation is done sth. in advance 1-2 days, and other affect trait is little.
2.2, the Helianthi fertilizer is to the influence (seeing Table three) of early rice economic characters
Table three is handled the influence to the early rice economic characters
Table three shows: the Helianthi fertilizer to early rice economic characters influences with effective fringe and the influence of the real grain of fringe number obviously and is compared the effective fringe of mu and is increased 0.7-0.8 a ten thousand/mu, and fringe is number increase 7.1-8 grain/fringe in fact, and also there is some difference for other character.
2.3, the Helianthi fertilizer is to early rice output and output value influence (seeing Table four)
Table is managed everywhere to early rice output and output value influence
Annotate: 100 kilograms of 190 yuan of meters of Oryza glutinosa.The Helianthi fertilizer is by 100 kilograms 120 yuan.
Table four shows: the Helianthi fertilizer on average increases production 22.9% to the early rice increase yield significantly, and amount of increase in production is 20.5%-25.1%, and output value benefit is obvious, goes into operation than average out to 1: 1.26, and amplitude is: 1: 1.4-1.3.
3, conclusion
3.1, the Helianthi fertilizer uses early rice fertility affect trait obvious in Oryza sativa L..Promote that early rice tillers and premature ripening.(seeing Table two)
3.2, the Helianthi fertilizer is used for early rice and can effectively improves the early rice economic characters, it is remarkable that effective fringe and the real grain of fringe number are increased effect.(seeing Table three)
3.3, the Helianthi organic fertilizer application is in early rice, it is remarkably productive to increase both production and value, unit on average increases production 22.9%, goes into operation than being 1: 1.26, is the good fertilizer that my area applies that suits.
Embodiment 4: with the plant extraction composite fluid allotment that obtains among the embodiment 1, use in aquatic products clarifier-tank water.
The plant extraction composite fluid that obtains among the embodiment 1 being allocated, used activated water as carrier (1: 10000) doubly, be added in the clarifier-tank water of aquatic products, to the residual removal of the medicine of aquatic products, also is the link that food chain is purified.Fig. 4 is the flow chart that aquatic products purify, as shown in Figure 4, the water source enters the mineral water treatment facility through the immersible pump motor, regulate and control plant extraction composite fluid electromagnetic valve simultaneously, make it to become the composite fluid of activated water allotment,, enter aquatic products and remove the residual purification water pool of medicine by the immersible pump motor, carry out oxygen supplement simultaneously, aquatic products are purified censorship in 24 hours, 48 hours.
(1) the common medicine of aquatic products is residual: concealed malachite green, chloromycetin, whistle base furan etc. are example with the mandarin fish, and the contained residual deal of medicine of aquatic products is measured in the mandarin fish after catching (in batches) i.e. censorship (sample).
(2) measure the residual deal of medicine after, this mandarin fish in batches is placed on adds in (1:10000) plant extraction composite fluid purification water pool doubly and oxygen supplement.After 24 hours, take out a sample censorship again, detect the residual deal of medicine and whether descend.
Following testing result is to have the Aquatic product company limited food safety control laboratory of qualification to do the report of the residual detection of medicine.
Detect for the first time:
Sample size: from 1 of feeding sample (catching censorship)
Date received ground: 2008.04.07
Reporting date: 2008.04.08
Sample size: from 1 of feeding sample (purifying censorship in 24 hours)
Date received ground: 2008.04.08
Reporting date: 2008.04.09
Sample size: from 1 of feeding sample (purifying censorship in 48 hours)
Date received ground: 2008.04.09
Reporting date: 2008.04.10
Detect for the second time:
Sample size: from 1 of feeding sample (catching censorship)
Date received ground: 2008.04.14
Reporting date: 2008.04.15
Sample size: from 1 of feeding sample (purifying censorship in 24 hours)
Date received ground: 2008.04.16
Reporting date: 2008.04.16
Sample size: from 1 of feeding sample (purifying censorship in 48 hours)
Date received ground: 2008.04.17
Reporting date: 2008.04.18
(3) this detection is an example to remove concealed malachite green, the residual examining report analysis of medicine, catch for the first time the concealed malachite green 8.36 that censorship is tested, handle the concealed malachite green 0.253 of censorship in 24 hours by purifying waste water, handle the concealed malachite green 0.218 of censorship in 48 hours by purifying waste water.Catch for the second time the concealed malachite green 0.476 that censorship is tested, handle the concealed malachite green 0.376 of censorship in 24 hours by purifying waste water, handle the concealed malachite green 0.248 of censorship in 48 hours by purifying waste water.From the changes of contents explanation of examining report concealed malachite green, the composite fluid of plant extraction liquid has pair effect of the residual removal of medicine.
Embodiment 5: the composite fluid of the plant extraction liquid that obtains among the embodiment 1 being allocated gained carries out toxicology test
Detect examining report (grinding [2007] 16) by Health Food Function Detection Center, Applied Literature and Science College, B.
Test item: health food two-stage toxicology test
Test basis: " health food check and assessment technique standard " (2003 editions)
Check conclusion: (trial target bioenergy health preserving water is composite fluid)
Under this laboratory condition, with concentration 25% bioenergy health preserving water to the maximum tolerated dose (MTD) of its mouse oral of two kinds of sexes all greater than 40mL/kgBW, true border innocuous substance.Through Salmonella reversion test, PCEMNR micronucleus test, mouse sperm deformity test, the result is all negative, is tried thing during table and does not have the mutagenesis activity.30 days feeding trials of rat, internal organs inspection, weighing, hematological examination result show that each dosage group of each inspection item and matched group more all do not have significant difference (P＞0.05), blood biochemical is learned and is checked that every biochemical indicator and matched group more all do not have significant difference (P＞0.05) in the index.The result of histopathologic examination shows, matched group and high dose group are female, male rat liver, kidney, stomach, intestinal, spleen, ovary and testis are not all found obvious damaging pathological change.
According to the criterion of " health food check with assessment technique standard " (2003 editions) as can be known, bioenergy health preserving water two-stage toxicological test is feminine gender as a result.
From check conclusion reflection, the plant extraction composite fluid that obtains among the embodiment 1 carries out consequently safety of health food two-stage toxicology test.
Embodiment 6: the plant extraction liquid that obtains among the embodiment 1 is allocated the analysis and assessment of the composite fluid of gained to former primary cultures of rat hepatocyte effect of vigor
By Peking University First Hospital's anesthesia pharmacological room's experiment (trial target bioenergy health preserving water is composite fluid)
Interpretation of result:
The method of two step perfusion isolating hepatocytes is to use maximum hepatocyte separation methods at present, and this method and technology is ripe, isolating hepatocyte output height, good dispersion degree.This experiment separates in this way and obtains disperseing hepatocyte preferably, and cell obtains most living cells behind rinsing, centrifugal purification, hepatocyte motility rate＞85% of trypan blue dyeing counting display separation.The hepatocyte condition of in vitro culture is had relatively high expectations, it not only will be selected proper culture medium, also will add some enzymes that promote hepatic cell growth and various cytokines simultaneously, as insulin, glucagon, epidermal growth factor, hepatocyte growth-promoting factors etc. in external growth well.This experiment intends observing hepatic cell growth and the proliferation function of test product bioenergy health preserving water to cultivating, therefore in the design of cell culture condition, do not add these compositions, and select nutrition DMEM culture medium preferably for use, in prepared culture, only added antibiotic and a certain proportion of hyclone.We are earlier with trypan blue colouring method counting hepatocyte motility rate after cultivating 3 days, find that hepatocyte does not have had significant proliferation, we begin formally to calculate the cell motility rate after 7 days, the sign that living cells does not have increase is respectively organized in experiment, we consider in the cell growth process except that hormone that needs various promotion cell proliferation and trophic factors etc., 3 kinds of test(ing) liquids that provide for this experiment are alkaline solution in addition, also should consider to contain in the test sample solution influence of factors such as higher concentration cation, make that just cell proliferation is slow even cause apoptosis.New isolating hepatocyte generally is in the resting stage of not having propagation, cell in the cultivation begins propagation or apoptosis under certain condition, mtt assay is the active common method of current detecting cell, its principle is that the succinate dehydrogenase in the living cells mitochondrion can make ectogenic MTT be reduced to the bluish violet crystal of slightly solubility, this crystal is deposited in the cell and colour developing, color that shows and viable count are relation in advance, this biochemical reaction of dead cell utilizes this principle can detect the living cells quantity of In vitro culture.The characteristics of MTT method be easy, quick, accuracy is high, extensively adopt under study for action.This experiment is used mtt assay instead and is detected discovery, the cell motility rate comparative control group of cultivating some group after 3 days increases, show that certain density detected test product has certain activation to hepatocellular propagation in the short-term cell cultivation process, along with incubation time prolongs, owing to lack the suitable culture condition, so the hepatocyte motility rate reduces gradually.
From interpretation of result, the plant extraction composite fluid that obtains among the embodiment 1 carries out the experiment to rat hepatocytes propagation, and its result has certain activation to hepatocellular propagation.
Embodiment 7: the composite fluid of the plant extraction liquid that obtains among the embodiment 1 being allocated gained is to the alleviating physical fatigue function test
By the test of the department of the Chinese Academy of Sciences of Department Of Medicine, Peking University's laboratory animal section, " the alleviating physical fatigue method of inspection " carries out in " the health food check and assessment technique standard " of the promulgation of test basis Ministry of Health of the People's Republic of China.(trial target bioenergy health preserving water is composite fluid)
(1) method of inspection
Swimming with a load attached to the body test: after last is drunk given the test agent 30min, put mice and in the depth of water is no less than the swimming case of 30cm, swim, 25 ℃ ± 0.5 ℃ of water temperature, the load sheet lead of 5% body weight of Mus tail heel, the record mice is the time to death from swimming, as the mice swimming time.With reference in " health food check and assessment technique standard " " the alleviating physical fatigue method of inspection ", the swimming with a load attached to the body experimental result positive.
(2) conclusion
Under this experiment condition, preliminary judgement bioenergy health preserving water has the effect of alleviating physical fatigue.
Embodiment 8: the anxious hepatic injury function test of giving birth to of composite fluid antagonism of the plant extraction liquid that obtains among the embodiment 1 being allocated gained.
By the test of the department of the Chinese Academy of Sciences of Department Of Medicine, Peking University's laboratory animal section, (trial target bioenergy health preserving water is composite fluid)
(1) method of inspection: after freely drinking water 23 days, three treated animals (wherein have one group and drink bioenergy health preserving water) prepare the acute liver damage model with 0.2ml/100g w rat skin lower injection 4% carbon tetrachloride, as a child get AST and ALT in the hematometry serum in back (fasting 12 hours) in injection carbon tetrachloride 20, and took by weighing liver weight.
(2) conclusion: under this experiment condition, judge that bioenergy health preserving water has the certain protection ability to acute liver damage.
Embodiment 9: the composite fluid of the plant extraction liquid that obtains among the embodiment 1 being allocated gained carries out drug-testing.
Detect (it is composite fluid that the trial target bioenergy is supported water) by national analeptic and sport nutrition testing research center, examining report numbering 07278
Test item: World Anti-Doping Agency's forbidden drugs
Testing result: this sample after testing, do not find stimulant, anesthetis, beta blocker, diuretic and the hormone medicine of World Anti-Doping Agency's regulation forbidding in 2007.
Embodiment 10: the plant extraction liquid that obtains among the embodiment 1 is allocated the clinical example of the composite fluid of gained to hepatovirus
The composite fluid that plant extraction liquid is allocated gained passes through zooblast experiment, anti-acute liver damage function animal experiment.For the composite fluid that further confirms plant extraction liquid carries out clinical trial to the reaction of hepatovirus.
(1) beam so-and-so, sex man at 32 years old age, was shown by clinical laboratory of Teaching Hospital No.3 of Zhongshan Medical Univ. immunity inspection report on March 29th, 2006:
So-and-so allocates beam the composite fluid of gained and rechecks by drinking plant extraction liquid, and assay is as follows:
Showed on August 2nd, 2006 by laboratory medicine portion of No.1 Hospital Affiliated to Zhongshan Univ. report:
(2) this patient's beam so-and-so, reached peace Clinical Laboratory center report by Guangzhou on April 28th, 2007 and show:
So-and-so allocates beam the composite fluid of gained and rechecks by drinking plant extraction liquid, and assay is as follows:
Reaching peace Clinical Laboratory center report by Guangzhou on June 13rd, 2007 shows:
(3) allocate the composite fluid of gained from above two report contrast reflection patients by drinking plant extraction liquid, its hepatovirus is had the positive therapeutic effect.
Claims (2)
1. the preparation method of a plant extract liquor is characterized in that, comprises the steps:
(1) extraction concentrates: with the aquatic foods product herb of Herba Portulacae, Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and the Herba Spinaciae section of chopping up or crush worn-out respectively, 1/4 kilogram of 1 kilogram or dry product become 5 kilograms of liquid, decocting time 4-7 hour with the 8-15 kilogram activated water decocting and concentrating of heating respectively;
(2) fermentation reaction treatment: 5 kilograms of concentrated extracts of Herba Portulacae described in the step (1), Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and Herba Spinaciae are respectively charged in the ceramic pot, after adding auxiliary material potting was closed 60-90 days, auxiliary material is 20-100 gram Caulis Sacchari sinensis juice and 10-20 gram Oryza glutinosa vinegar;
(3) antagonism stabilized treatment: the liquid after the Herba Portulacae in the step (2), Herba Ecliptae, Herba Bidentis Bipinnatae, Herba Medicaginis, Flos Chrysanthemi, Fructus Vitis viniferae and the Herba Spinaciae fermentation is removed precipitate respectively and get liquid 2-3 kilogram, every kind of liquid is done mutual antagonism stabilized treatment 60-90 days respectively;
(4) centrifugal: liquid 1-2 kilogram on it is got for every group in the stable back of plant concentrated extract antagonism, and the reuse centrifugal method is repeatedly centrifugal, gets the about 0.5-1 kilogram of liquid mixing preparation on it for every group.
2. plant extraction liquid that utilizes the described preparation method of claim 1 to obtain.
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| CN104774087B (en) * | 2015-03-27 | 2017-12-12 | 深圳柏施泰环境科技有限公司 | Biological agent and preparation method thereof and the application in soil improvement, fertilizer |
| CN106904750A (en) * | 2017-03-28 | 2017-06-30 | 河南省长义农业科技有限公司 | Phytoenzyme depollution of environment liquid prepared by a kind of use plant rubbish and its preparation method and application |
| CN108991041A (en) * | 2018-06-15 | 2018-12-14 | 黄少飞 | A kind of public place special plant extraction thimerosal and preparation method thereof |
| CN111874968A (en) * | 2020-07-17 | 2020-11-03 | 广东顺德正善川生物科技有限公司 | Method for enhancing wastewater treatment by adopting plant extract |
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