CN110140808A - Application method of the tea seed cake as aquatic livestock and ruminant additive - Google Patents

Abstract

The application field that the present invention relates to tea seed cakes in feed solves previous tea seed cake usable problem of ability after aquatic products material will go (de-) poison or toxicity reduction with the use ruminated in material；Inventor by tea seed cake it is accurate it is qualitative for aquatic products and the feed addictive of ruminant rather than feedstuff, it is believed that add suitable tea seed cake and be not only safe, and be effective.It in aquatic feeds and ruminates in feed, by the relation schema of tea seed cake dosage and effect, is determined as Hormesis mode (i.e. hormesis mode)；The amount ranges of tea seed cake are determined as, 0.1--1 kilograms is added in fish material per ton, 0.5-6 kilograms are added in shell-fish aquatic products material per ton, per ton ruminate adds 0.5--6 kilograms in fine fodder.This usage and dosage can improve daily gain, improve feedstuff-meat ratio, increase the output of milk, reduce death and culling rate；The method is safely, effectively again convenient.

Description

Application method of the tea seed cake as aquatic livestock and ruminant additive

Technical field: the invention belongs to agricultural byproducts processing application field, it is related to a kind of using tea seed cake as aquatic products and ruminating The application method of animal additive can range the purposes invention of agricultural and sideline product.

The term name involved in the present invention arrived, spy are explained as follows and explain；If the term and industry of this patent meaning Term has conflict, complete general character or common part, and approval is given in invention per capita；If the definition of industry slang and intension itself are simultaneously It is unintelligible or when without standard common recognition, it is subject to the following definition of inventor, and preferred explanation that make generalization or expansionary, removes It is non-to be otherwise noted.

1. tea oil tree: also referred to as camellia tree refers in Theaceae (Theacea) Camellia (Camellia.Linnaeus) plant Seed oil content is higher, fruit is used to extract oil, and has certain cultivation to manage the perennial arbor of area or the general designation of shrub species. Including being distributed most wide C. olelfera (C.Oleifera Abel), camellia meiocarpa (C.meiocarpa), Yunnan camellia (C.reticulata), Camellia chekiangoleosa (C.chekangoleosa), Camellia yuhsienensis (C.yuhsiensis) and Camellia Vietnamensis (C.vietnamensis) etc. local oil tea also includes having both the ornamental and difunctional all kinds of camellia trees of oil-producing.

2. tea tree: also referred to as tea tree refers to Theaceae (Theaceae) Camellia Camellia Sinensis (Linnaeus) O.Kuntze plant；Its leaf or bud can directly steep drink after suitably processing with boiled water；Including green tea (Longjing tea, green tea, Huang Shan Maofeng, all even high-quality green tea etc.), black tea (β-glucosidase, souchong, broken black tea etc.), oolong tea (extra-strong tea, Wuyi Rock tea, phoenix single tea etc.), white tea (silver tip pekoe tea, white peony tea, tribute mee tea etc.), (Meng Dinghuang young tea leaves, Huoshan are yellow for yellow tea Young tea leaves, Junshan Silver Needle Tea tea etc.), dark green tea, all kinds of tea trees such as Pu'er tea.

3. tea seed: being the seed of tea fruit including camellia seed (also referred to as tea seed) and tea seeds；Tea seed episperm is (also referred to as in tea seed Tea inner casing) account for about the 1/3 of tea seed total weight, tea seed kernel (also referred to as tea benevolence) accounts for about the 2/3 of weight.

4. tea Pu: crust or shell including camellia fruit (camellia oleifera fruit) and tea seeds fruit；Pericarp is in oblate or olive more Olive shape.

5. tea seeds: the fruit for all kinds of tea (leaf) tree drunk in i.e. daily is the byproduct of Tea Production.

6. tea oil: using tea seeds or oil tea (camellia) seed as raw material, the oil that is obtained by the methods of squeezing and/or extracting Product.

7. cake: for resulting byproduct after mechanical-physical squeezing；The dregs of rice: resulting by-product after taking oil is leached for chemical solvent Product.

8. tea cake: referring to the byproduct using tea seeds or camellia seed as raw material, after extracting oil, there is pie and sheet.

9. tea seed cake: also referred to as tea bran or tea seed cake or tea seed cake slag.Refer to broad sense in this patent, including Oil-tea-cake, Extracted From Oil-tea-cake, The tea seeds dregs of rice and tealeaves seedcake are the general names for processing tea seed and taking the solid leftover bits and pieces after oil, belong to byproduct, and do not pass through It crosses such as detoxification, the reprocessing of other techniques such as detoxification or fermentation；It not only include that leaching method takes all kinds of byproducts after oil；Also include Tea cake in term 8 substantially can be considered same materials with regard to the powdering dregs of rice after crushing because of cake.Because Producing Area Soil, weather, kind, The difference of processing method and detection method etc., there is some difference for main active ingredient content in tea seed cake, tea in general tea seed cake The content of saponin is 10% or more, high reachable 18% or so or higher, and customary amount is 15% or so；Tannin (tea polyphenols) contains Amount is 1.5% or more；It is usually all in puce or filbert；There are a variety of physical aspects such as pie, sheet, particle, fine-powdered；? Including band Extracted From Oil-tea-cake and without Extracted From Oil-tea-cake；Band grass tea seed cake and without careless tea seed cake.

10. Tea Saponin Tea saponin: also referred to as tsubakisaponin or saponin(e, be Theaceae Camellia Plants (including oil tea and Tea tree etc.) in all kinds of saponin unified address；Belong to the homology of the similar Triterpenoids sapogenins saponins of main structure Mixture；The general character with saponin.Tea Saponin has toil pungent, it will be apparent that stimulation schneiderian membrane, and have many characteristics, such as hemolytic, quilt Some experts are considered anti-nutritional factors.

11. tea polyphenols Tea Polyphenols: referring to all kinds of positions in tea (leaf) tree and tea oil tree, molecule knot There are the plant component general name, including tannin, flavonoids etc. of several phenolic hydroxyl group in structure；It may be from tealeaves or tea seed cake or tea Pu Deng.

12. tannin Tannin: also referred to as tannin belongs to the scope of tea polyphenols.Tannin is that a kind of water-soluble, molecular weight exists Phenolic compound between 500~3 000；Hydrolysable Tannins and condensed tannin can be divided by chemical structure tannin；Condensed tannin Acid is flavane 01 derivatives, and 2 of flavanols are by carbon-carbon bond in conjunction with catechol or benzenetriol in molecule；Hydrolysable Tannins Acid has ester bond in molecule, is the gallate of glucose.Tannin is negatively charged bioactive molecule, list in easy and saliva Chemical reaction (hand-gloves mode) occurs for positive charge protein, and oral cavity surface layer can be made to generate convergence sense, feel dry and astringent.Tea seed cake list Ning Yinqi astringent taste is considered anti-nutritional factors by some experts.

13. procyanidine Proanthocyanidins: namely condensed tannin, by different number catechin in structure (catechin) or epicatechin (epicatechin) is combined into.Simple procyanidine be catechin or epicatechin or The dimer that catechin and epicatechin are formed.By degree of polymerization size, two~pentamer is usually known as oligomer (referred to as OPC), by pentamer referred above to high polymer (abbreviation PPC).

14. flavone compound Flavonoids: narrow sense refers to that parent nucleus is a kind of compound of 2- phenyl chromone, referred to as Flavone compound.Usually yellow, 4 have ketone carbonyl group, and that most common is exactly 2- phenyl chromone (2- phenyl benzo γ Pyranone) class, i.e. flavonols；Broad sense refer to one kind made of all two phenyl ring (A ring, B ring) are interconnected by three carbochains at Part is known as flavone compound.Mostly with the basic framework of 6C-3C-6C, and often there are hydroxyl, methoxyl group, methyl, isopentene group Equal substituent groups.Flavone compound includes above two definition in this patent.

15. the detection method of component content and relevant item in tea seed cake product, if can be carried out by with laxative remedy without specified otherwise Detection.

1) Tea Saponin is detected by HG/T 4492-2013 or by the method for detecting total saposins in GB/T25247-2010.

2) tea seed cake is detected the volume of produced foam by GB/T 7462.

3) tannin is by the national standard of tannin content or Edible taunin measurement in GB/T15686-2008 sorghum.

4) tea polyphenols detect: carrying out by GB/T 8313-2008 method.

16.Hormesis: Chinese is translated into hormesis or low dosage promotes effect；It is for describing virulence factor Technical term.This dose-effect relationship model, neither most threshold models is applied in traditional poisonous substance risk assessment (threshold model), nor linear non-threshold model (linear non-threshold model), but two-phase agent Graded effect model, intoxicating factor (such as noxious material) has organism when alternatively referred to as U-shaped or J-type curve model, i.e. high dose Evil, and it is beneficial to organism when low dosage.

Background technique: China is oil tea big country, and annual tea oil yield is about 500,000 tons according to statistics, while adjoint and generation About 1,500,000 tons 3,000,000 tons nearly with tea Pu of oil tea byproduct tea seed cake.For oil tea how long in southern hilly area, the two byproducts are deep Processing ratio is all little；Some are used to extract Tea Saponin tea seed cake, and the tea Pu overwhelming majority is taken as fuel to burn up.

China is also to produce tealeaves big country simultaneously, and can produce tea seeds every year according to statistics is about 800,000 tons, and about 100,000 tons of tea leaf oil； Ten thousand tons of the nearly 60-70 of tea seeds grouts generated together simultaneously.Tea seed oil is also obtained as new resource food 2009 simultaneously Ministry of Public Health's approval, the byproduct of tea seed oil, i.e. the tea seeds dregs of rice are also more and more.

Status: in recent years, people are more for the exploitation concern of camellia oil and tea seed oil, while opening two class tea seed cakes Hair utilizes also to be paid attention to gradually.From the point of view of two class tea seed cake compositions, contain about 12-18% crude protein, the total reducing sugar more than 30%, mineral The multiple components such as prime element, it should feedstuff, but the tea due to usually containing higher concentration (10-18%) in tea seed cake can be become Saponin, a certain amount of tannin, the alkaloid and flavones of 1.5-3% and 30% or so cellulose, have strong impulse With pungent bitter taste, palatability is very poor；Especially Tea Saponin and tannin etc. are considered anti-nutritional factors by some experts.Therefore it raises Expect in industry, most technical staff, generally believes that tea seed cake fodder firstly the need of solution is detoxification problem, that is, removes tea Detoxification or detoxification tea seed cake after the anti-nutritional factors such as saponin and tannin, can just use in feed.Such as " Chinese herding magazine " " it is feeding that microbe fermentation method improved Extracted From Oil-tea-cake in 2014 by 4th phase in 1987 " experiment to feed pigs after tea seed cake detoxification " and Diao Huan Quality progress ".

The method of tea seed cake detoxification has hole to bury fermentation method, alkali disintoxication, water extraction, solvent method at present.It is de- that fermentation is buried using hole Poison needs dig pit, seal, and needs the long period；Alkali disintoxication needs to be added aqueous slkali, boils, filters, drying, alkali disintoxication Protein can also be destroyed；Water extraction needs to be added hot water, heat preservation, centrifugation elimination solution, extracts, dries repeatedly；Extract by solvents Tea Saponin is needed repeatedly to extract tea seed cake crushing, addition such as ethyl alcohol, also to be dried again.Other strain fermentation detoxification detoxification Technology be still not perfect, some methods are not only cumbersome time-consuming and ineffective, at present all be lab scale or pilot scale stage, all do not have It realizes that technical scale metaplasia produces, is not more widely popularized and applied.

If the general los biosynthesis scientific & technical corporation Yang Zhonghua of Rui'an City is in the Publication No. 104286383A of application in 09 month 2014 16 Patent " a kind of leached tea oil slag poison-removing method "；Agricultural University Of Anhui Cai Haiying is in the Publication No. of application in 04 month 2013 17 103211084A patent " preparation method of the tea seed cake fermented feed rich in biological enzyme ".Anhui Dabieshan Technology Development Co., Ltd. A kind of patent " high-quality tea seed cake for feed addition of the Publication No. 105028920A of application in remaining light bright 08 month 2015 05 Slag and preparation method thereof " tea seed cake is sent out using mixed bacteria (bacillus subtilis, lactic acid bacteria, saccharomycete and actinomyces) Ferment.

At present major part tea seed cake be still taken as organic fertilizer, fish pond pool-cleaning agent or fuel come using；Also some is used As raw material, the Tea Saponin of high concentration is therefrom extracted, the industries such as knits, dig up mine for washing, pesticide, building materials.

Technical regulation knowledge related to the present invention:

According to " feed and feed addictive management rules ".

Article 17 is forbidden to use the feedstuff catalogue of administrative department in charge of agriculture's announcement, feed addictive kind mesh Any substance other than record and medicated feed additive kind catalogue produces feed.

Article 23 forbids managing the feedstuff catalogue with administrative department in charge of agriculture's announcement, feed addictive product The feed of any substance production other than kind catalogue and medicated feed additive kind catalogue.

Up to the present, the Ministry of Agriculture announce No. 1773 and No. 2038 promulgation " feed feedstuff catalogue " and Tea seed cake is not all found in " catalogue of feed additive varieties (2013) " of No. 2045 publication；Be likely to in feedstuff industry Generally existing technology prejudice is related, i.e., tea seed cake has strong impulse and pungent bitter taste (being commonly called as toxic), and palatability is very poor, Animal food refusal is related；Feed industry overwhelming majority technical staff generally believes that tea seed cake cannot be directly used to feed, to use, first Necessarily solve the problems, such as detoxification or detoxification；Just because of above-mentioned two reasons, cause tea seed cake not on fill the catalogue used perhaps；Tea simultaneously The dregs of rice also not in medicated feed additive kind catalogue, i.e., tea seed cake temporarily all there are no be put into feed allow using catalogue In legal use；

Up to the present, it does not find, tea seed cake is directly added into the document report used in ruminant feed；Do not have yet It finds directly by tea seed cake, by 0.1-1 kilograms of fish material of addition per ton, 0.5-6 kilograms of shell-fish aquatic feeds are in aquatic feeds Using and obtain good result document report.

Row expert is just keen to, and using different process and method, all kinds of active materials is extracted from tea seed cake, such as tea soap Element, tea polyphenols or tannin, flavone compound, tea seed polysaccharide, tea seed albumen etc. have simultaneously delivered a large amount of technical article, application Many patents of invention." a kind of high-purity tea saponin as filed in Hefei Xinda film Science and Technology Ltd. on 08 20th, 2017 Extracting method " patent；Xinyang Normal College's Hua Rui institute height is into bravely equal to " a kind of Extracted From Oil-tea-cake filed in 18 days 01 month in 2016 The fast preparation method of middle flavonoid glycoside ".

It may be since tea seed cake is considered as low-grade agricultural and sideline product, so far, tea seed cake itself also never has national It defends planning commission and is approved as new resource food, food additives, the food of healthy food material or food and medicine consangunity is domestic also any There is the habit of edible tea seed cake in place, that is, generally believes tea seed cake inedibility；Need to extract wherein corresponding effective active components, It is possible to human consumption or makees medical usage.

In addition tea seed cake is the agricultural and sideline product of edible oil factory, also not in the Ministry of Agriculture and defends the product blacklist that planning commission is forbidden to use On.

Summary of the invention: application method of the tea seed cake as aquatic livestock and ruminant additive.

It describes in detail first to several main active ingredients of tea seed cake in the present invention:

The first main active ingredient Tea Saponin.The content range of Tea Saponin has plenty of 10-15% in document report tea seed cake, Some is 12%--18%, even 20%；The reason of this species diversity: on the one hand related with the places of origin of raw materials and processing technology；Separately On the one hand related with the detection method of Tea Saponin；In gravimetric method detection, the detected value of ethanol extraction method is extracted than n-butanol The detected value of method wants high；This is because can also contain other compositions such as flavonoids, tannin in ethanol extract；And n-butanol extracts It is mainly exactly Tea Saponin in object.Tea Saponin content is high in tea seed cake, there is very strong toil pungent, easily schneiderian membrane is stimulated to cause sneeze； Palatability is poor, and hygroscopicity is stronger.

Zhang Xinghai 2003 " Tea Saponin property and application study recent developments " and river and source 2007 " property of Tea Saponin, Preparation and application " article in all introduce, Tea Saponin is a kind of natural nonionic surfactant, have well stable cream Change effect, foaming capacity, also there are the drug effects such as the unexistent sterilization of other surfaces activating agent, anti-inflammatory, analgesia, impervious.

The article of Zhang Xinghai is also mentioned that Tea Saponin can stimulate the secretion of animal hormone in vivo, such as adrenal gland and matter hormone (skin Matter sterone), it since corticosterone is very strong to carbohydrate metabolism, can promote gluconeogenesis and gluconeogenesis, inhibit the benefit of blood glucose With, therefore blood glucose can be made to be temporarily increased.Tea Saponin can also reduce the cholesterol levels in liver and serum, thus have reducing blood lipid work With.

" the biological activity of Tea Saponin and its in animal productiong of Zhan Yong patent ZL96193005.5 and Hao Yuanhua 2015 Application study progress " all refer to that Tea Saponin has enhancing immunocompetence, and have effects that oxidation resistant.

Second of composition tea polyphenols, refers mainly to tannin.Content is 0.5--5% in tea seed cake；Tea seed cake tannin is because its astringent taste is by one A little experts are considered anti-nutritional factors；But the polyphenol hydroxyl chemical structure and unique chemical property of tannin make tannin show compared with Strong oxidation resistance.Additionally while tannin can mutually be adsorbed with carbohydrate, amino acid, protein and iron ion in feed etc., but this Kind absorption is reversible, and is easily desorbed in acidic environment.Tannin has been approved for food additives；Vegatable tannin is usual It is considered safe.Tannin also belongs to the scope of tea polyphenols, and tea polyphenols have been put into national standard GB2760-2007 " food additive Agent is added to use sanitary standard " in, major function is antioxidant.Open source literature introduces oil tea tannin also and has antibacterial and disease-resistant Poison, the multiple efficacies such as resisting cardiovascular disease.Using having effects that natural antibacterial anti-corrosion in field of food, as stabilizer It can be used in liquid beverage, be alternatively arranged as the raw material of health food.

The third active ingredient flavonoids.Flavones content has been reported that about 0.2% in tea seed cake, and also some is reported as 1.3%, there may be substantial connection with detection method, have bitter taste；There is open source literature to show the flavone compound in tea seed cake simultaneously Mainly Kaempferol, naringenin and its derivative etc..The text that such as Chen Hongxia is delivered in 2011 " chemistry of forest product and industry " In chapter " separation of flavonoid glycoside compound and Structural Identification in cake of camellia oleifera seeds ", it is indicated that flavonoid glycoside is mainly kaempferia galamga in cake of camellia oleifera seeds Phenol 3-O- [2-O- β-D- galactolipin -6-O- α-L- rhamnose]-β-D-Glucose glycosides and be Kaempferol 3-O- [2-O- β-D- wood Sugar -6-O- α-L- rhamnose]-β-D-Glucose glycosides.

Hou Liuxin etc. published an article in " Food Science " a kind of " the separation mirror of novel tea seed flavones monomer in 2013 Fixed and its antioxidant activity " in, be separated to new flavone compound, i.e. naringenin -7-O- (β-D- xylopyranosyl (1 → 6)) (β-D-Glucose base (1 → 3)-α-L- rhamnopyranosyl (1 → 2))-β-D-Glucose glycosides.Rivers source etc. was in " tealeaves in 2005 Science " on publish an article " in teaseed cake dregs flavonoid glycoside HPLC analysis, preparation with MS identify " in, it is indicated that the Huang in teaseed cake dregs Ketoside mainly has kaempferol-3-O-rutinoside and Kaempferol -3-O- (6-O- β-D- glucopyranosyl；)-β-D- galactolipin Two kinds of glycosides.

Simultaneously in a kind of Wang Chengzhang granted patent 101899070B " system of flavonoid glycoside in middle compression leg quick separating cake of camellia oleifera seeds Preparation Method " and Gao Jinyong number of patent application 201610027047.6 " fast preparation method of flavonoid glycoside in a kind of Extracted From Oil-tea-cake " text In offering, introduce in tea seed cake also containing flavonoids natural activity composition, have it is anti-inflammatory, it is anti-oxidant, the effect of female hormone sample and Antibacterial and antiviral and other effects, and vasodilation can be made, protect heart and brain；Improve memory, anti-depression anxiety and neuroprotection etc. are more Kind physiological activity, flavonoids is existing to be much developed to novel plant medicine, such as Total Ginkgo Flavone-Glycoides, and has the work of solution toxin for liver With.

4th kind of active ingredient: ceramide.In addition Japan Patent JP2004189683 and Guilin Sun Buxiang is awarded It is described in power patent 102058727B " tea seed extract containing ceramide and preparation method thereof " in tea seed cake also containing one kind Natural ceramide, and refer to that ceramide is a kind of bimolecular lipid layer component, belong to the lipid structure unit of sphingomyelins One of, there is important biological action, such as courier's effect and active cell apoptosis, is adjusting Apoptosis, cell differentiation, transhipment And proliferation etc. has certain function.Somebody thinks such as controlling cancer, neurasthenia, diabetes, obesity and inflammation It can also play a role Deng during.

The alkaloids such as the 5th kind of active ingredient caffeine.The caffeine for containing about 1% in tea seed cake, belongs to xanthine alkaloid Compound；Containing alkaloid about 1.5-3%, (nitrogenous alkaline organic compound, majority have complicated cyclic structure, nitrogen in tea seed cake In ring) its biological activity may be natural with other organic compounds containing nitrogen (such as vitamin B1), have it is more Kind physiological function.

Alkaloid has bitter taste, and such as caffeine, it is also a kind of central nervous excitation agent simultaneously, and appropriate intake is extremely beneficial 's.

In addition there are also tea seed albumen about 12-18% in tea seed cake, tea polysaccharide is about more than 30% and beneficial trace mineral is first Element.

Generally existing significantly technology prejudice in feedstuff industry: because tea seed cake has strong impulse and pungent bitterness Taste, palatability is very poor, influences animal feed intake or even animal food refusal is related (being commonly called as toxic)；So feed industry overwhelming majority skill Art personnel generally believe that tea seed cake cannot be directly used to feed, and tea seed cake can just be used in feed only after detoxification or detoxification；Simultaneously This technology prejudice has guided many technical staff to go to develop various techniques and go detoxification or detoxification or toxicity reduction.

Up to the present, (do not make any extraction, not detoxification or toxicity reduction or detoxification) after tea seed cake being crushed, add by fine fodder per ton The dosage for adding 0.5-6 kilograms is applied directly to use in ruminant fine fodder, and obtains the document of good effect, there are no always Open report.

In aquatic feeds, although the article " research that Oil-tea-cake tentatively feeds fish " for having Yang Shengchang nineteen ninety to deliver, real Oil-tea-cake after using detoxification or detoxification on border.Publishing an article within Li Hongquan 2005, " Extracted From Oil-tea-cake feed is supported in hybridized prussian carp Application study in growing ", actually use the detoxification Extracted From Oil-tea-cake after water leaching extracts Tea Saponin.

There is Liang Shizhong to publish an article on the 13rd phase in 2015 " Chinese feed " " the non-detoxification tea of different pitch-based spheres recently Influence of the seedcake to Growth Op Tilapia performance ", conclusion be additive amount of the non-detoxification tea seed cake in tilapia feed be 3 grams/ Kilogram, when this dosage of tea seed cake, the gaining effect of fish is best；But it is to be worth paying special attention to or suspect that this article, which has at following 5 points, :

First point, test tea seed cake does not have specific examining report and analysis data, in tea seed cake Tea Saponin and other The content of composition such as tannin is how many actually, has no way of finding out about it.Test tea seed cake used is any all non-detoxification actually Tea seed cake or the halfway so-called tea seed cake of detoxification, source have no introduction, there is certain query.

Second point, table one, which is infused, in article contains 10% Tea Saponin with tea seed cake to calculate the addition of Tea Saponin in feed Amount, but do not mention this thing for testing Tea Saponin dosage in article, the addition of Tea Saponin is not mentioned in conclusion yet Amount；In the case where no any detection data, 10% Tea Saponin is just contained with tea seed cake to calculate, this be it is obvious relatively low, Leave a question open.

Thirdly, Yang Shengchang (1990) is mentioned in article discussion 3.1 feeds Tilapia mossambica and week jade-like stone etc. with 40% tea seed cake etc. (1988) soft-shelled turtle is fed, what is used is all the tea seed cake that detoxification is crossed, but still claims to be tea seed cake；Therefore the fish examination of Liang Shizhong tea seed cake It tests, with tea seed cake used in Yang Shengchang and Zhou Ying, it should not have comparativity and similitude, be before and after the definition of tea seed cake here Inconsistent.

4th point, Francis etc. (2002) is mentioned in article discussion 3.2 and adds 300mg/kg soap in Tilapia mossambica daily ration Glucoside, weight gain are significantly raised.There are three serious mistakes for the statement, and 1. original text is not Tilapia mossambica (Tilapia) but carp (Carp)；2. saponin used in the test is not genuine soap glucoside (glycosides), but purchases the S2149 from Sigma company, content is to use What sapogenin (Sapogenin) indicated, factory guarantee value is no less than 10%, according to Quillaja saponin(e average molecular weight 1500 or so and the ratio of the sapogenin molecular weight about more than 500 convert, saponin content is about 33% or so, i.e., It is about 100mg/kg that Francis, which tests saponin amount used, rather than 300mg/kg.Furthermore 3. the fish body of 300mg/kg saponin group Weight gain, is not obvious in fact.Specifically refer to the article " Tea Saponin pair that Li Nengqin is published in " agriculture in south " the 6th phase in 2013 The influence of carp growth performance " second segment of page 82.That is the Quillaja saponin(e to carp addition 150mg/kg such as Francis When weight gain significantly improve, feed conversion rate and metabolic efficiency all significantly improve；Add the Quillaja soap of 300mg/kg Weight gain is unobvious when glycosides.

5th point: the resulting conclusion of Liang Shizhong article is, dosage of the non-detoxification tea seed cake in tilapia feed be 3 grams/ Kilogram, this dosage, fish gaining effect is best；Initially guessed and calculated by it, 10% Tea Saponin is contained with tea seed cake to calculate and raise The additive amount of Tea Saponin in material, just and 300mg (tea saponin)/kg；This Francis thought with him (2002) is in Tilapia mossambica 300mg/kg saponin is added in daily ration, the apparent conclusion of weight gain is precisely identical；But the practical Francis that he thinks (2002) conclusion be it is wrong, be specifically shown in and analysis at above-mentioned 4th point.

Wrong Producing reason is analysed in depth and deduces repeatedly, the possible first step is to think saponin in fish material with firsting impressions are strongest In dosage should be set in 300mg/kg, this dosage be rationally, this has foreign literature as reference frame, but actually this It is the serious erroneous judgement to English initial data；Second step is to carry out experimental design on this basis, with tea seed cake contains 10% Tea Saponin will reach the concentration requirement of saponin 300mg/kg in fish meal, then need per ton as theoretic calculating basis In fish meal add 3 kilograms of tea seed cakes, this be typically by fruit retrodict because experimental design thinking.Because its mistakenly with reference to The thinking of 300mg/kg saponin is added in Francis (2002) fish daily ration, conclusion is very forced and there are many places suspicious；Industry simultaneously It is interior there is the tea seed cake (tea seed cake) for extracting Tea Saponin is also referred to as tea seed cake or feed grade tea seed cake, and detoxification tea seed cake The appearance of non-detoxification tea seed cake be it is quite similar, need to detect or take any special measures could distinguish and identify, thus invention People has good reason to suspect whether its so-called non-detoxification tea seed cake is really non-detoxification；If test is really added to non-detoxification Tea seed cake, then 3 g kgs of the dosage of tea seed cake and gaining effect are with regard to suspicious.

(any extraction or deep processing are not made after tea seed cake is crushed at present；Not detoxification or toxicity reduction or detoxification) directly press fish per ton The usage that 0.1 kilogram to 1 kilogram of feed addition is come using and obtaining the document of good effect, there are no open report always.

Inventing the technical issues of being required to solution is: the pungent bitter taste of tea seed cake, palatability is very poor (being commonly called as poisonous and harmful), The problem that can not be directly used in aquatic feeds and ruminant.

The technical solution of inventor: for the first time in aquatic feeds and ruminant feed, Hormesis mould is used to tea seed cake The effect of type (two-phase Dosg-response Model, low dosage promote effect) Lai Kexue studies and judges tea seed cake, the accurate dosage for analyzing tea seed cake and Relationship between effect；Determine amount ranges of the tea seed cake in feed per ton are as follows: 0.1 kilogram to 1 kilogram of fish material；Shell-fish aquatic products 0.5 kilogram to 6 kilograms of feed；0.5 kilogram to 6 kilograms of ruminant fine fodder；Optimal use dosage should be located at above-mentioned corresponding simultaneously The position of intermediate dosage；Above-mentioned problem can be solved using above-mentioned low dosage, it is not only harmless, it is beneficial instead, fishes and shrimps etc. can be improved The growth performance of aquatic livestock, such as weight gain improve, and case fatality rate reduces；For ruminant, the output of milk can be improved, promotes life It is long, improve feed efficiency.

Inventor is after reading domestic and international lot of documents and combines working experience, sums up a few points of the invention:

One: there are four possible causes of technology prejudice in the understanding to tea seed cake in the industry.

1. it is the agricultural and sideline product for having certain toxicity that tea seed cake, which is generally believed that, should not directly be used in feed；This understanding Based on existing experience and animal experiment case, it is really reasonable；But this toxic understanding and understanding so-called to tea seed cake are General and fuzzy, mainly remain in the toxic and nontoxic traditional cognitive stage.Influence angle from tea seed cake to animal feed intake It is judged from the point of view of degree, according to traditional poisonous substance risk model, the toxicity of tea seed cake may be to belong to the model for having threshold value really (threshold model), rather than linear non-threshold model (linear non-threshold model).Because of feed industry Technical staff seldom knows about Hormesis model and accurately recognizes, and it is even more impossible to carry out science judgment；So being difficult from another One angle (as improved growth of animal performance) assert that the toxic model of tea seed cake is also possible to belong to Hormesis model, i.e. two-phase Dosg-response Model.

2. it is toxic why other tea seed cake is generally believed by personnel in feedstuff industry, can not directly use；Main cause is to be worked as Make feedstuff using.Fine analysis concrete reason is as follows: first is that tea seed cake is as the dregs of rice class such as dregs of beans, rapeseed meal, it is all edible oil factory Byproduct；Second is that all containing more crude protein, third is that tea seed cake is cheap, the more members of about 2000--3000 per ton are easy purchase； Therefore feed those skilled in the art are considered as feedstuff easily to treat, once as raw material, the dosage in feed It is easy for bigger than normal, starting point dosage is exactly 1-3%, i.e., 10--30 kilograms of tea seed cake of addition per ton, if not prior detoxification or detoxification, just have It is toxic to be commonly called as tea seed cake for pole apparent side effect, slow growth, therefore tea seed cake can not use in feed when raw material.

3. tea seed cake can not be used directly, but technical staff often bears that tea seed cake price is again cheap, and crude protein content is again in mind constantly Height, and be easy to purchase, therefore want by every possible means using tea seed cake, for your other raw material substituted, to reduce cost；But because containing The anti-nutritional factors such as more Tea Saponin, tannin just may be used only after by tea seed cake detoxification or toxicity reduction (as extracted Tea Saponin or fermentation) It is used as feedstuff；Moreover tea seed cake can add 5--10% or more, and the effect energy of animal experiment in feed after detoxification Receive, cost can reduce again, therefore this usage has certain market.Especially factory is in the Tea Saponin for extracting high added value Afterwards, the price of remaining (after detoxification) tea seed cake is per ton 2,000 yuan still nearly, i.e. the price differential of tea seed cake and (detoxification) tea seed cake is less obvious；Factory More there are driving force and enthusiasm, extract the Tea Saponin of high added value, a kind of tea seed cake is become into two class products in this way, economic benefit is bright Aobvious to increase, manufacturer imitates one after another, and a large amount of detoxification tea seed cake (slag i.e. after extraction Tea Saponin) listings are taken as feedstuff to use； Feed industry utilizes the problem of tea seed cake, is solved indirectly in a way, tea seed cake fodder problem is no longer urgent.

4. tea seed cake is really very cheap in addition from price, per kilogram only has 2--3 member, also just than raw material such as dregs of beans etc. Preferably；Therefore practitioner in feed, it is subjective to be easier to for tea seed cake to be classified as raw material class, as the dregs of rice classes such as dregs of beans rapeseed meal cotton dregs；Thing For this major premise with regard to inclined, tea seed cake functional localization is fixed wrong in reality, should not be regarded as raw material use；It's the wrong direction, naturally with regard to easy one Mistake hundred is wrong.

Two: inventor can be in global correction feedstuff industry to three main points of tea seed cake technology prejudice:

1. inventor substantially deeply carefully analyzes from tea seed cake, determine that a variety of unique activity compositions contained by tea seed cake are special Sign, corresponding effective content feature and corresponding efficacy characteristics (such as bactericidal, anti-inflammatory, anti-oxidant, raising immunity), this Three kinds of feature its technical characteristics for more meeting feed addictive completely；Although according to several surface characteristics of tea seed cake (dregs of rice class, The byproduct of edible oil factory, containing more crude protein, price is also cheap), tea seed cake, which is classified as raw material class also, reasonable place；But Comprehensive assessment is got off, and according to the main notable feature and comparative advantages feature of tea seed cake, first choice should be classified as functional feed addition Agent class, rather than feedstuff class；Positioning, location of mistake should not more be obscured.

2. tea seed cake has bactericidal, anti-inflammatory, kills the virus, is anti-oxidant, improving and exempt from from intrinsic activity composition and content The effect of epidemic disease power；From the practical application effect of this patent, tea seed cake has really promotes fishes and shrimps and ruminant growth, improves Daily gain improves material ratio, reduces tangible effect of case fatality rate；Therefore tea seed cake is a kind of obvious effect and exact plant source adds Agent can partially replace or substitute completely in the market for resisting somatotrophic plant to mention additive, and tea seed cake is really quality-high and inexpensive green Colour additive.

3. inventor is after understanding hormesis (Hormesis) model in depth；It and is to understand tea seed cake in analysis After the main function mechanism and practical work experience of middle active ingredient；The toxic model for confirming tea seed cake is Hormesis model, It is harmful to organism when also referred to as two-phase Dosg-response Model, i.e. high dose, and it is beneficial to organism when low dosage；This poisonous substance Amount effect relation curve be also referred to as U-shaped curve or low dosage and promote Effective pattern.The present invention needs scope of protection namely Tea seed cake low dosage promotes the dosage use scope in effect, i.e., theoretic minimum effective dose (Lowest Observed Effect Level) range without visible destructive dose (NOAEL, No Observed Adverse Effect Level) is arrived, The tea seed cake dosage protection scope being equivalent in this patent, the amount ranges in feed per ton, fish material are from 0.1 to 1 kilogram；First 0.5 to 6 kilograms of aquatic feeds of shell class；0.5 to 6 kilograms of ruminant fine fodder.

Three: the inventive principle and invention main points that inventor summarizes have following ten:

1. inventor is from the comparative advantages feature of tea seed cake (common tea seed cake contains Tea Saponin 15% or so, 0.5% or more tannin) Three technical characteristics summed up, i.e., rich and varied active ingredient feature, effective content feature and actual efficacy feature are assert Tea seed cake should first choice be classified as functional additive；Without feedstuff etc should be belonged to.

2. in inventor, tea seed cake is all treasured from head to foot in the eyes, and contained there are many natural activity compositions, such as Tea Saponin, tea polyphenols Including tannin (containing procyanidine) and flavones, ceramide and caffeine Alkaloid etc., wide in variety and content is higher； These unique active ingredients, naturally get together, and may have the born synergistic effect evolved, must not take When arduously extracted or extracted with organic solvent；Moreover active ingredient be to be difficult to extract completely by solvent 's；Some activity just decline or all forfeitures during the extraction process.Tea seed cake is directly appended to use in feed, all activity and battalion It supports, is lost without any composition or activity declines；All effective ingredients all fully utilize, and play respectively unique life Object activity.

3. inventor thinks that the tannin in tea seed cake has stronger polarity, easily combined to form not soluble in water answer with protein Close object；Tannin-protein complex be in pH4.0-7.0 it is stable, can then be decomposed rapidly below or above this range, and For the rumen ph of ruminant between 5.0-7.0, protein is more stable in conjunction with tannin, is not easy to be degraded by microorganisms.Work as list When rather-protein complex flows through abomasum (pH2.5) and small intestine (pH8.0-9.0), protein is separated with tannin, through stomach cardia Enzyme and trypsin degradation, protein degradation forms the small-molecular peptides for being easy absorption, therefore tannin makes in ruminant feed With playing the protective effect of class by-pass protein to a certain extent.

4. inventor thinks that Tea Saponin and tannin can damage ruminant because of its specific molecular structure of chemistry in tea seed cake The integrality and functionality of protozoon cell membrane in cud, to prevent, inhibit even to destroy rumen metabolism, and by the original in cud Borer population amount makees reasonable limitation, and protozoon, which will reduce the phagocytosis of bacterium, to be slowed down, and is conducive to increase all kinds of fibres in cud in this way Tie up the breeding of decomposer；Bacterial number in one side cud will increase, so that mycoprotein amount will increase；On the other hand to thick Feed includes that can all increase to the utilization rate of entire feed.If the dosage of certain tea seed cake is excessive, protozoon in cud will lead to Quantity sharply reduce, the balance being also unfavorable between the stabilization of Rumen Internal Environment and various micropopulations, thus function of rumen Decline, the output of milk also reduce.The excessive feed intake for also influencing ruminant of tea seed cake amount simultaneously.

5. the main reason for inventor thinks one of the active ingredient in tea seed cake Tea Saponin, plays effect is that its is excellent The feature of nonionic surfactant, while there is hydrophilic radical, and have lipophilic group, and with good stability；Tea soap Element is not needed to be rapidly absorbed into intestinal cell and just be played a role；Tea Saponin majority is that active function is just played in enteric cavity, 1. Tea Saponin is able to suppress or kills some common parasitic worms of fish enteron aisle.2. very strong very stable emulsification, can promote oil The digestion and absorption of rouge；3. can be in conjunction with the cholesterol on intestinal cell mucous membrane, activating cell Lipid Rafts enhances the penetrating of cell membrane Property, promote absorption of the enterocyte to nutriment；4. enteron aisle relevant cell can be activated, enhance enteron aisle resistance

6. inventor can accurately recognize with the hormesis model of Scientific application tea seed cake (two-phase Dosg-response Model or U-shaped mode), i.e., high dose when to organism nocuousness, and it is beneficial to organism when low dosage；In conjunction with the animal experiment of related tea seed cake As a result, scientific analysis, comprehensive analysis: minimum effective dose (LOEL, Lowest Observed Effect Level), best effective Fruit dosage (Maximum response level) and without visible destructive dose (NOAEL, No Observed Adverse Effect Level) three proportionate relationships between key value and three dose values；NOAEL value is averagely approximately LOEL value 10-20 times；Optimal dose is averagely approximately 1/5th of NOAEL value.

7. comprehensively consider active ingredient content in tea seed cake, it is dialectical between dosage and using effect of the tea seed cake in feed Relationship；The scientific and reasonable quality standard for formulating tea seed cake product, the recommendation usage and dosage and effect standardized in feed are expected.

8. key point of the invention is to correct for technology prejudice, i.e., tea seed cake it is toxic can not directly be used in feed it is inclined See；Tea seed cake is accurately positioned as functional feedstuff additive simultaneously, rather than values the albumen in tea seed cake to be considered as feed former Material；In feedstuff industry, tea is studied and judged using hormesis model (two-phase Dosg-response Model) Lai Kexue to tea seed cake for the first time The effect of dregs of rice；(if Tea Saponin content is 15% or so in conventional tea seed cake, tannin is no less than to accurate analysis effective component content 0.5%), the relationship between different dosages and actual functional capability effect three.

9. the standard content of effective ingredient in tea seed cake, accurate reasonable recommendation dosage is arranged to scientific rationality, it is contemplated that real Existing effect；Reach the safe and convenient to use efficient of tea seed cake, the institute for really largely playing tea seed cake comprehensively is powerful；And make Product quality keeps stabilization not fluctuate；The production of product can be made to realize standardization simultaneously, specialized, scale and industry Change.

10. the present invention has extremely strong practicability, it is very beneficial for realizing tea seed cake upgrading of industries；Tea seed cake is functional The accurate positionin of feed addictive, added value are promoted rapidly；And it will greatly be conducive to later period tea seed cake direct food additive Change, tea seed cake health care product, or even directly drug；Specially run business big and strong tea seed cake industrial chain to do, establish sturdy theoretical grounding in basic skills and The practical application experience of accumulating and enriching.

Supplementary explanation: patent application document disclose or invention patent mandate after plan.

Inventor intends to be first to file patent protection, and reporting product certification afterwards or submitting a report asking for be included in allows using catalogue.Invention is special Benefit is to push and promote the progress of entire science and technology, there is certain perspective and primacy, the product invented (including technology Scheme or operational version), it is possible to it does not temporarily acquire state approval also or is included in permission catalogue, this is to be understood that completely 's.

It applies for a patent after and file qualified in acquisition first trial discloses or after license, before having certain rights protection It puts, can actively prepare every data and report, to national related administrative service division, declaring, which allows using or obtain administration, is permitted Can certification；Then practical investment implements (licensed-type production and/or sale) again.

The technological merit and technical effect of invention:

One tea seed cake is from a wealth of sources, largely quickly can produce and realize industrialization；The byproduct of numerous tea growers obtains height simultaneously Effect utilizes, and demand can be bigger, and tea seed cake not only can more sell well, while price can may also be gone up, and tea grower's income can be It improves.

Two tea seed cake powder processing costs are low or zero cost.Producer inherently produces is tea seed cake fine powder, it is only necessary to it is simple Be replaced with feed grade label, can direct marketing, use technical solution of the present invention almost zero cost.If by tea cake or coarse granule Tea seed cake is processed into tea seed cake fine powder, and production method is also very simple, production process zero emission and pollution；Tea seed cake all makes full use of；? Only needing common grinder, crushing and processing cost is very low (the 100--200 member per ton that about increases crushes cost), almost without threshold, Be easy to learn and use easy popularization.The sale of tea seed cake is because tea seed cake can be sold and be used by feed addictive in feed, i.e. tea seed cake Purposes expands or changes, and user group surges；From cannot be to energy, from zero to one, this be the variation of matter, this is cleverly to innovate；Cause The amount that this tea seed cake directly sells to feed industry has the increase advanced by leaps and bounds；The economic benefit of tea seed cake manufacturer can dramatically increase.

Three is easy to use and use cost is low.Tea seed cake product is directly added in feed, it is not necessary to detoxification, it can be directly low for a long time Dosage addition, method is easy, and use cost is low, disease-resistant growth-promoting significant effect and definite.It simultaneously can partially or completely substitute valence The much more expensive other plants of lattice mention feed addictive, can obviously save cost；Growth promotion antibiosis can also be partially or completely substituted simultaneously The use of element, reduces the residual and pollution of antibiotic.

Four tea seed cake products can be widely used in animal feed, be used for a long time, and to environment without any pollution.

Five tea seed cake products can antibacterial diseases prevention, growth promotion, at the same also have anti-inflammatory, it is anti-oxidant, enhance animal immunizing power Effect；It is pure natural and non-harmful, is the prospect that is rich in for anti-growth promotion green product.Not only quality-high and inexpensive, even more object is super It is worth；Using technical solution of the present invention, be benefited in many ways, either tea seed cake manufacturer or client (feed or breeding enterprise) It is all easy to adopt technical solution of the present invention, is to achieve many things at one stroke, the good thing of multi-party co-benefit；Therefore it is readily available big face It long-pending popularization and generally uses, with good economic efficiency, ecological benefits and social benefit.

Six present invention are scientific and simply solve technical problem of the tea seed cake for feed, in the range of recommending dosage, Tea seed cake can promote the growth of the aquatic animals such as fishes and shrimps, improve weight gain, reduce case fatality rate；Ruminant can be promoted to grow, improved The output of milk；Do not have to worry tea seed cake toxic side effect again simultaneously；The invention can effectively and rationally utilize tea seed cake resource, be to benefit the nation and the people Philanthropic act.

The specific technical solution of the present invention is following (application method):

The preparation method of tea seed cake product:

1) all kinds of tea seed cakes (including cake, piece, particle) are purchased, impurity foreign matter is removed, dries or dry；

2) mechanicalness crushing is carried out to above-mentioned raw materials in room temperature, is ground into about 80--150 mesh；

3) method detection Tea Saponin content and National Standard Method detect tannin (can also survey tea polyphenols) content by weight, and keep a record；

4) projects such as other conventional ingredients (such as crude protein and ash) and sanitary index in testing product, and keep a record.

5) after check is qualified, the label of corresponding grade is sticked, and be packaged.

The application method of tea seed cake product:

With conventional tea seed cake (as set quality standard as Tea Saponin content is not less than 15%, and tannin content is not less than 0.5%) As premise and basis.According to the two-phase Dosg-response Model of tea seed cake Hormesis theory, the low dosage of tea seed cake promotes in effect Dosage range, be that minimum effective dose (LOEL) theoretically is arrived without visible destructive dose (NOAEL), and NOAEL Value is 10-20 times of LOEL value；Optimum efficiency dosage is 1/5th or so of NOAEL value.

In conjunction with the test data in the embodiment of the present invention, amount ranges of the tea seed cake in feed per ton, fish material be from 0.1 to 1 kilogram；0.5-6 kilograms of shell-fish aquatic feeds；0.5--6 kilograms of ruminant fine fodder.Although amount ranges with it is theoretic There is a certain error for LOEL value and NOAEL value, but between two numerical value proportionate relationship also close to 10 times；This numeric ratio Example relationship is substantially identical with Hormesis theory；This has also fully demonstrated two-phase Dosg-response Model, therefore tea seed cake exists Aquatic feeds and the optimum addn amount in fine fodder is ruminated, it should be near the intermediate dosage in above-mentioned corresponding amount ranges.

Specific embodiment

In order to state that technical solution of the present invention more accurately, below in conjunction with the embodiment of the present invention, to the present invention Technical solution be clearly and completely described.Obviously, described embodiment is a part of the embodiments of the present invention, without It is whole embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creative work Under the premise of every other embodiment obtained, shall fall within the protection scope of the present invention.

Embodiment one: tea seed cake product preparation method

1) tea seed cake of Changshan County, Zhejiang Province is purchased.

2) carry out mechanicalness crushing, powder at 120 mesh or so fine powder.

3) Tea Saponin and tannin content therein, Tea Saponin 15.6%, tannin content 1.9% are detected.

4) corresponding label is sticked, and it is spare to mark detection and analysis value.

Embodiment two: the preliminary test of fish selects similar in size weight carp 200, averagely about 90 grams or so；At random It is divided into 5 groups；Every group of 40 fishes；Blank control group (tea seed cake T0 is not added in foundation bait)；Four test groups, respectively in foundation bait The tea seed cake 100 (T1) of the upper acquisition of embodiment one of addition respectively, 300 (T2), 600 (T3), 1000 (T4) mg/kgs, experimental period 45 days；Other feed intake and manage in the usual way, and growth performance and survival rate of the tea seed cake to carp of various dose are added in observation The influence of (or death rate).Test result is shown, compared with blank control group, the fish of tea seed cake group, average weight gain is respectively increased 3.47% (T1), 13.62% (T2), 6.45% (T3) and 2.38% (T4)；In addition each group death toll is 6 (T0), 3 respectively Item (T1), 2 (T2), 1 (T3), 1 (T4), PRELIMINARY RESULTS shows: 0.1-1 g kgs of tea seed cake is added in fish meal, It can promote the healthy growth of fish, and the most obvious in the gaining effect of 0.3 g kg of dosage group with tea seed cake dosage；Feed simultaneously Middle addition tea seed cake can enhance the resistance of fish body, it is only dead reduce fish, and with the survival rate highest of 0.6,1 g kg of dosage group.

Embodiment three: the preliminary test of shrimp selects similar in size weight Penaeus Vannmei 240, every average weight It is 6.5 grams or so, is randomly divided into 6 groups, every group of 40 shrimps carries out 30 days culture experiments.(basal feed is not added blank control group Tea seed cake T0)；Five test groups add tea seed cake 0.5 (T1), 1 (T2), 2 of the acquisition of embodiment one respectively on foundation bait respectively (T3), 4 (T4), 6 (T5) g kg, other feed intake and manage in the usual way, the tea seed cake prawn of observation addition various dose Growth performance and survival rate (or death rate) influence.Test result is shown, compared with blank control group, the shrimp of tea seed cake group is flat 4.53% (T1), 11.75% (T2), 17.28% (T3), 15.34% (T4), 2.37% (T5) is respectively increased in weight gain；In addition The death toll of each group shrimp is 8 (T0), 5 (T1), 4 (T2), 3 (T3), 2 (T4), 2 (T5), PRELIMINARY RESULTS respectively Show: adding 0.5-6 g kgs of tea seed cake in shrimp feed, the growth of shrimp can be promoted, and with tea seed cake dosage 2--4 grams/thousand The gaining effect of gram dosage group is more obvious；Abwehrkraft des Koepers can be enhanced by adding tea seed cake simultaneously, reduce the death of shrimp, and death toll Have the tendency that reducing as tea seed cake dosage increases.

Example IV: the test of milk cow selects Fresian similar in 24 outputs of milk and lactation number of days, average daily The output of milk is randomly divided into 6 groups, every group 4 at 31 kilograms or so；That is blank control group (tea seed cake T0 is not added in fine fodder) and five examinations Test group, additionally added in fine fodder respectively various dose embodiment one obtain tea seed cake: 0.5 g kg (T1), 1 g kg (T2), 2 g kgs (T3), 6 g kgs (T5), is tested 35 days by a definite date, other are carried out in the usual way by 4 g kgs (T4). Test result shows: compared with T0 group, the average daily milk production of test group is respectively increased 0.17 kilogram (T1), and 0.34 kilogram (T2), 1.59 kilograms (T3), 1.62 kilograms (T4), 0.93 kilogram (T5).This explanation adds a certain amount of tea in cow concentrate The dregs of rice can improve the dairy performance of milk cow really, and are advisable with adding 1--6 g kgs of tea seed cake in fine fodder, and the dosage of tea seed cake It is preferable for effect with 2--4 g kgs.

Embodiment five: the test of sheep selects goat similar in 30 weight, about 16 kilograms of average weight or so, random to divide At 6 groups, every group 5；Blank control group (tea seed cake T0 is not added in fine fodder) and five test groups, additionally addition is different in fine fodder respectively The tea seed cake that the embodiment one of dosage obtains: 0.5 g kg (T1), 1 g kg (T2), 2 g kgs (T3), 4 g kgs (T4), 6 g kgs (T5) is tested 30 days by a definite date, other are carried out in the usual way.Test result shows: compared with T0 group, examination The weight gain for testing group is respectively increased 0.27 kilogram (T1), and 0.84 kilogram (T2), 1.59 kilograms (T3), 1.35 kilograms (T4), 0.46 thousand Gram (T5).This explanation adds a certain amount of tea seed cake in the fine fodder of sheep, can improve the growth performance of sheep really, and with tea seed cake in essence Additive amount, which is 1--6 g kgs, in material is advisable, and tea seed cake dosage is preferable for effect with 2--4 g kgs.

Test Summary: dosage of the tea seed cake in aquatic feeds and ruminant fine fodder is controlled in a certain range, to dynamic Object health and growth performance are beneficial, such as weight gain raising, output of milk increase；Facilitate to reduce simultaneously dead；This may be by Sterilization composition such as Tea Saponin and tannin contained in tea seed cake, the Polyphenols isoreactivity substance such as flavones are increased with dosage, Sterilizing ability is remarkably reinforced related.By the above test result, can be used with preliminary judgement tea seed cake in the low dosage recommended above When, it is generally beneficial；This low dosage for also complying with Hormesis theory promotes effect model.

Production method of the invention simultaneously is simple, easy to operate；Some powder products can be used directly without reprocessing；This The production method of invention, no pollution zero-emission, complete utilization belong to green and trust health-oriented products；It is environmental-friendly when production, when use It is ecological friendly, non-pollution discharge, no hazard residue；Sustainable development；Either Feed Enterprise still cultivates unit, affords to use, With obtaining, with value；Growth promotion protection effect is definite, and the present invention is easy to promote and universal, and not only tea grower and tea oil factory are directly benefited, Numerous raisers are also benefited simultaneously simultaneously, achieve many things at one stroke, turn waste into wealth, benefit the nation and the people.

Embodiments described above is exemplary embodiments of the invention, for illustrating technical solution of the present invention And technical effect, whole possibility embodiments can not be covered, nor the limitation carried out to the present invention.

The present invention is corrected to there is technology prejudice in aquatic feeds industry and ruminant feed for many years, is used Hormesis model, i.e. low dosage promote effect by the toxic non-serviceable wrong views of the tea seed cake accumulated in industry, thoroughly entangle Just come；Formally establish tea seed cake low dosage using beneficial scientific ideology, within the scope of the present invention, i.e., often Tea seed cake is added in ton feed, fish material adds 0.1--1 kilograms, and crust aquatic feeds (Shrimp waste) add 0.5-6 kilograms；It ruminates dynamic 0.5--6 kilograms of technical solution is added in object fine fodder, to any simple modification and adjustment that technical solution of the present invention is made, such as Adding carrier dilution etc., both falls within protection scope of the present invention.

Significantly enlightenment, those of ordinary skill in the art are not making based on the embodiments of the present invention and directly simultaneously Under the premise of creative work, by dosage control of the tea seed cake in complete feed per ton, (fish material 0.1-1 is public in the reasonable scope Jin；0.6-6 kilograms of shell-fish aquatic feeds；0.5--6 kilograms of ruminant fine fodder) technical solution, by same principle and think of Road is extended in other aquatic animal feeds and ruminant feed, such as sheep, beef cattle other corresponding realities obtained Technical solution and other equivalent or equivalent simple alternatives are applied, shall fall within the protection scope of the present invention.

Claims (10)

1. a kind of application method of tea seed cake in aquatic feeds, which is characterized in that added tea seed cake as the feed of aquatic livestock Add agent come using, not as aquatic livestock feedstuff come using.

2. application method according to claim 1, which is characterized in that use of the tea seed cake in aquatic livestock complete feed per ton Measuring range is 0.1-1 kilograms of fish material, 0.5-6 kilograms of shell-fish aquatic feeds.

3. application method according to claim 1, which is characterized in that tea seed cake dosage in aquatic livestock complete feed per ton Range are as follows: 0.3-0.6 kilograms of fish material, 2-4 kilograms of shell-fish aquatic feeds.

4. a kind of application method of tea seed cake in ruminant feed, which is characterized in that be using tea seed cake as ruminant feed Additive come using, not as ruminant feedstuff come using.

5. application method according to claim 4, which is characterized in that dosage model of the tea seed cake in ruminant fine fodder per ton Enclose is 0.3-6 kilograms.

6. application method according to claim 4, which is characterized in that dosage model of the tea seed cake in ruminant fine fodder per ton Enclose is 2-4 kilograms.

7. -6 tea seed cake according to claim 1, which is characterized in that tea seed cake refers to plant of theaceae seed, including tea seed and Tea seeds, the remaining byproduct after taking oil.

8. -6 tea seed cake according to claim 1, which is characterized in that be not less than than content for hundred parts of Tea Saponin weight in tea seed cake 10%.

9. -6 tea seed cake according to claim 1, which is characterized in that the content of hundred parts of ratios of tannin weight is not less than in tea seed cake 0.5%.

10. tea seed cake described in -6 according to claim 1, which is characterized in that the active ingredient in tea seed cake refers to including Tea Saponin, list Rather, tea polyphenols, flavone compound, ceramide and the like.