CN106389541A - Cistanche deserticola extract and preparation method and application thereof - Google Patents
Cistanche deserticola extract and preparation method and application thereof Download PDFInfo
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- CN106389541A CN106389541A CN201610950842.2A CN201610950842A CN106389541A CN 106389541 A CN106389541 A CN 106389541A CN 201610950842 A CN201610950842 A CN 201610950842A CN 106389541 A CN106389541 A CN 106389541A
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- 239000000284 extract Substances 0.000 title claims abstract description 61
- 238000000605 extraction Methods 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 241000336291 Cistanche deserticola Species 0.000 title abstract 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000004365 Protease Substances 0.000 claims abstract description 28
- 238000001035 drying Methods 0.000 claims abstract description 22
- 239000004310 lactic acid Substances 0.000 claims abstract description 19
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 108091005804 Peptidases Proteins 0.000 claims abstract description 16
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 16
- 230000004060 metabolic process Effects 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 3
- 102000004190 Enzymes Human genes 0.000 claims description 39
- 108090000790 Enzymes Proteins 0.000 claims description 39
- 229940088598 enzyme Drugs 0.000 claims description 39
- 230000000694 effects Effects 0.000 claims description 34
- 241000005787 Cistanche Species 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 27
- 239000000843 powder Substances 0.000 claims description 26
- 239000003814 drug Substances 0.000 claims description 21
- 239000000706 filtrate Substances 0.000 claims description 17
- 239000000047 product Substances 0.000 claims description 14
- 235000019419 proteases Nutrition 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 13
- 235000013305 food Nutrition 0.000 claims description 12
- 235000013372 meat Nutrition 0.000 claims description 12
- 108090000145 Bacillolysin Proteins 0.000 claims description 11
- 108091005507 Neutral proteases Proteins 0.000 claims description 11
- 102000035092 Neutral proteases Human genes 0.000 claims description 11
- 108091005658 Basic proteases Proteins 0.000 claims description 9
- 108090000526 Papain Proteins 0.000 claims description 8
- 239000012567 medical material Substances 0.000 claims description 8
- 229940055729 papain Drugs 0.000 claims description 8
- 235000019834 papain Nutrition 0.000 claims description 8
- 239000008213 purified water Substances 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 7
- 108010004032 Bromelains Proteins 0.000 claims description 5
- 235000019835 bromelain Nutrition 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 239000002552 dosage form Substances 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 5
- 239000012676 herbal extract Substances 0.000 claims description 5
- 230000002265 prevention Effects 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000002775 capsule Substances 0.000 claims description 3
- 239000007919 dispersible tablet Substances 0.000 claims description 3
- 239000006196 drop Substances 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000003094 microcapsule Substances 0.000 claims description 3
- 230000000116 mitigating effect Effects 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 239000006187 pill Substances 0.000 claims description 3
- 239000007901 soft capsule Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000006188 syrup Substances 0.000 claims description 3
- 235000020357 syrup Nutrition 0.000 claims description 3
- 239000003826 tablet Substances 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims description 2
- 210000000496 pancreas Anatomy 0.000 claims 2
- 239000008176 lyophilized powder Substances 0.000 claims 1
- 230000002929 anti-fatigue Effects 0.000 abstract description 7
- 238000009835 boiling Methods 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 241000252212 Danio rerio Species 0.000 description 29
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 12
- 230000008569 process Effects 0.000 description 11
- 229940079593 drug Drugs 0.000 description 7
- 230000033001 locomotion Effects 0.000 description 7
- 206010002660 Anoxia Diseases 0.000 description 6
- 241000976983 Anoxia Species 0.000 description 6
- 206010021143 Hypoxia Diseases 0.000 description 6
- 230000007953 anoxia Effects 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 235000010265 sodium sulphite Nutrition 0.000 description 6
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- 235000014103 egg white Nutrition 0.000 description 4
- 210000000969 egg white Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 108010019160 Pancreatin Proteins 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 241000411851 herbal medicine Species 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 229940055695 pancreatin Drugs 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
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- 235000013402 health food Nutrition 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000336316 Cistanche tubulosa Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000283070 Equus zebra Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008558 metabolic pathway by substance Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000107 myocyte Anatomy 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/64—Orobanchaceae (Broom-rape family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to Cistanche deserticola extract and a preparation method and application thereof. The Cistanche deserticola extract is made by mixing fresh or dry Cistanche deserticola with water, enzymatically extracting, concentrating the extract, and drying. The preparation method comprises: air-curing fleshy stems of Cistanche deserticola, crushing and screening, adding water, stirring, adjusting temperature and pH, adding a biological protease for enzymatic hydrolysis extraction, boiling after extracting, centrifuging and filtering, and concentrating and drying the supernate to obtain the Cistanche deserticola extract. The Cistanche deserticola extract prepared herein is capable of significantly improving in-vivo lactic acid metabolism and improving mobility, and thereby has significant anti-fatigue action.
Description
Technical field
The invention belongs to health-oriented products field, it is related to a kind of preparation method of Herba Cistanchess extract, and its in terms of resisting fatigue
Application.The extract of the present invention can use as food, health food or medicine.
Background technology
Fatigue is the result that body is led to through complicated physiological acoustic signals process.From central nervous system to skeleton
Myocyte arrives intracellular substance metabolism process again, and the comprehensive change of any one link middle or process all can cause tired
Labor.Fatigue not only reduces the original ability to work of body, and points out body to develop into a certain degree of sick and wounded state.People
After body produces fatigue state, if cannot timely treating and eliminating, it will gradually accumulate, ultimately result in overwork, occur slow
Fatigue syndrome.
Modern society many people, because fatigue is in sub-health state, have had a strong impact on work efficiency and life matter
Amount.Therefore, find the focus that effective anti-fatigue medicament is health care personnel and scientist's concern in recent years.Due to changing
Learn the Physiological and Biochemical Metabolism that synthetic drug disturbs human body, cause serious toxicity and research costs dearly, countries in the world are just
Emphasis is turned to natural drug.In the international trend of " mankind go back to nature ", the long conventional medicament theory base of China
Plinth and practical experience and abundant Chinese herbal medicine resource are even more and attract attention for the world, and from Chinese herbal medicine, searching toxic and side effects are little, safety
Effectively anti-fatigue medicament oneself become the study hotspot of pharmacy circle.
It is contemplated that finding a kind of plant extract with preferable anti-fatigue effect, it is the meat with Herba Cistanches as raw material
Herba Cistanchess Cistanche Herba also known as golden Radix Crotalariae szemoensis, Herba Stellariae Saxatilis, Herba Cistanchess, big rue, are orobanchaceae plant cistanche Cistanche
The dry zone scale leaf of deserticola Y.C.Ma or Cistanche Tubulosa Cistanche tubulosa (Schrenk) Wight
Fleshy stem, main product is in the Inner Mongol, Gansu, Xinjiang, Qinghai and other places.Beginning is loaded in《Sheng Nong's herbal classic》, it is classified as top grade, classic thereafter
《Bencao Jingshu》、《Collected statements on the herbal foundation》、《Moonlight son book on Chinese herbal medicine》Deng how on the books.Li Shizhen (1518-1593 A.D.) is said：" this thing is mended and not high, thus have calm
".Herba Cistanches sweet in the mouth, salty, warm in nature, there is the function of kidney-replenishing, benefiting essence-blood, loosening bowel to relieve constipation, in existing more than 2,000 year of China
Usage history.Modern pharmacology shows, Herba Cistanches in addition to as traditional kidney invigorating and YANG supporting Chinese medicine, more with defying age, increasing
The several functions such as hypermnesia is recalled, enhance immunity.The present invention prepares Herba Cistanchess extract using special process, lives through vivo biodistribution
Property tests confirmation, and it has significant anti-fatigue effect.
Content of the invention
The invention discloses a kind of preparation method and its usage of Herba Cistanchess extract, by Herba Cistanches fleshy stem dried and crushed
Afterwards, obtained using enzyme extraction method and there is the Herba Cistanchess extract of biological activity, this extract can serve as food, health food and
Medicine.The preparation technology that the present invention plants is to summarize out through long-term scientific research, and its feature is process is simple, warp
Ji, environmental protection, it is simultaneously used for large-scale production.
For reaching above-mentioned purpose, the present invention is realized by following methods：
A kind of Herba Cistanches extract, it is prepared by the following method：Herba Cistanches fresh goods or dry product are mixed with water, enters
Row enzyme extraction, the relative density that extracting solution is concentrated into when about 30 DEG C is 1.05～1.10g/mL, and drying obtains extract.
Herba Cistanches are optionally gone up or under ground portion, and wherein aerial partss preferably are selected from leaf, flower, stem or seed.
Preferably, after Herba Cistanches meat stem portion is dried, pulverize, sieve, add water stirring, adjust temperature and pH, add life
Thing protease carries out enzymolysis and extraction, after the completion of extraction, boils, and is centrifuged, filters, the concentrated drying of supernatant, obtains khaki desert
Rong's extract.
Preferably, the meat stem portion in Herba Cistanches medical material is dried and is pulverized by (1), crosses 40 mesh with upper screen cloth thus obtaining
Herba Cistanchess medicated powder.
(2) a certain amount of Herba Cistanchess medicated powder is mixed for 1: 10～1: 20 in mass ratio with purified water, add medicated powder quality
0.1%～0.5% bio protease, adjusts pH to 5～9,40～55 DEG C of stirring 3～6h of constant temperature；
(3), after the completion of extracting, the herbal extract in step (2) is boiled 5～10min, is cooled to room temperature, was centrifuged
Filter, filtrate is stand-by；
(4) by the filtrate in step (3) temperature be 50～60 DEG C be concentrated into about 30 DEG C when relative density be 1.05～
1.10g/mL, drying obtains khaki Herba Cistanchess extract；The yield of described Herba Cistanchess extract is not less than 40%.Preferred steps
(3) filtrate in is 50～60 DEG C before concentrating
Preferably, described bio protease is selected from the neutral protease (enzyme activity >=200,000 u/g) of food stage, Fructus Chaenomeliss egg
White enzyme (enzyme activity >=400,000 u/g), bromelain (enzyme activity >=300,000 u/g), alkaline protease (enzyme activity >=200,000 u/
G), one of pancreatin (enzyme activity >=3000u/g) or their mixture, it is preferred to use neutral protease, alkaline egg
White one of enzyme, papain or their mixture；
Preferably, described method for concentration can concentrate for membrance concentration, concentrating under reduced pressure, normal pressure；
Preferably, described drying meanss can be spray drying, vacuum drying, heat drying, lyophilization.
Present invention also offers a kind of extracting method of Herba Cistanches extract, it comprises the steps：By Herba Cistanches fresh goods
Or dry product is mixed with water, carry out enzyme extraction, the relative density that extracting solution is concentrated into when about 30 DEG C is 1.05～1.10g/mL, through dry
Dry obtain extract.
Preferably, described extracting method comprises the steps：
(1) the meat stem portion in Herba Cistanches medical material being dried and pulverized, crossing 40 mesh with upper screen cloth thus obtaining Herba Cistanchess medicine
Powder；
(2) a certain amount of Herba Cistanchess medicated powder is mixed for 1: 10～1: 20 in mass ratio with purified water, add medicated powder quality
0.1%～0.5% bio protease, adjusts pH to 5～9,40～55 DEG C of stirring 3～6h of constant temperature；
(3), after the completion of extracting, the herbal extract in step (2) is boiled 5～10min, is cooled to room temperature, was centrifuged
Filter, filtrate is stand-by；
(4) by the filtrate in step (3) temperature be 50～60 DEG C be concentrated into about 30 DEG C when relative density be 1.05～
1.10g/mL, drying obtains khaki Herba Cistanchess extract；The yield of described Herba Cistanchess extract is not less than 40%.
Preferably, described bio protease is selected from the neutral protease (enzyme activity >=200,000 u/g) of food stage, Fructus Chaenomeliss egg
White enzyme (enzyme activity >=400,000 u/g), bromelain (enzyme activity >=300,000 u/g), alkaline protease (enzyme activity >=200,000 u/
G), one of pancreatin (enzyme activity >=3000u/g) or their mixture, it is preferred to use neutral protease, alkaline egg
White one of enzyme, papain or their mixture；
Preferably, described method for concentration can concentrate for membrance concentration, concentrating under reduced pressure, normal pressure；
Preferably, described drying meanss can be spray drying, vacuum drying, heat drying, lyophilization.
Present invention also offers a kind of compositionss, it contains said extracted thing, and pharmaceutically acceptable or edible adjuvant；Can be any
Dosage form, preferably it is tablet, dispersible tablet, capsule, soft capsule, microcapsule, granule, injection, injectable powder, freeze-dried powder
Agent, pellet, drop pill, syrup, powder, extractum, soft extract, oral liquid and other pharmaceutically acceptables
Dosage form.
Described extract and compositionss can improve lactic acid metabolism in organisms thus playing the effect of resisting fatigue.
Described compositionss can be used for preparing the medicine of relevant disease, the food that prevention, treatment or mitigation cause because of fatigue
Product or health product.
Preferably, the preparation method of the Herba Cistanchess extract of the present invention, it comprises the steps：Herba Cistanches meat stem portion is dried in the air
After dry, pulverize, sieve, add water stirring, temperature and pH are adjusted to after the optimum condition of bio protease enzyme extraction, add a certain amount of
Bio protease carry out enzymolysis and extraction, after the completion of extraction, boil inactivation, centrifugation, filter, the concentrated drying of supernatant, obtain
Khaki Herba Cistanchess extract, concrete preparation method is as follows：
(1) the meat stem portion in Herba Cistanches medical material being dried and pulverized, crossing 40 mesh with upper screen cloth thus obtaining Herba Cistanchess medicine
Powder.
(2) a certain amount of Herba Cistanchess medicated powder is mixed for 1: 10～1: 20 in mass ratio with purified water, add medicated powder quality
0.1%～0.5% bio protease, adjusts pH to 5～9,40～55 DEG C of stirring 3～6h of constant temperature.
(3), after the completion of extracting, the herbal extract in step (2) is boiled 5～10min, is cooled to room temperature, was centrifuged
Filter, filtrate is stand-by.
(4) by the filtrate in step (3) temperature be 50～60 DEG C be concentrated into about 30 DEG C when relative density be 1.05～
1.10g/mL, drying obtains khaki Herba Cistanchess extract.
Described bio protease is selected from the neutral protease (enzyme activity >=200,000 u/g) of food stage, papain (enzyme
Vigor >=400,000 u/g), bromelain (enzyme activity >=300,000 u/g), alkaline protease (enzyme activity >=200,000 u/g), pancreatin
One of (enzyme activity >=3000u/g) or their mixture, it is preferred to use neutral protease, alkaline protease, wood
One of melon protease or their mixture.
Described method for concentration can concentrate for membrance concentration, concentrating under reduced pressure, normal pressure.
Described drying meanss can be spray drying, vacuum drying, heat drying, lyophilization.
The yield of described Herba Cistanchess extract is 40～60%.
Present invention also offers a kind of compositionss, it contains described Herba Cistanchess extract, acceptable on medicine or food helps
Agent.
Preparing gained Herba Cistanchess extract in this patent described can be with the medicine such as Huang of conventional prevention or treatment fatigue diseases
The compounded combination such as essence, Fructus Lycii.
Prepare gained Herba Cistanchess extract in this patent described to combine with pharmaceutic adjuvant, it is tablet, dispersible tablet, capsule
Agent, soft capsule, microcapsule, granule, injection, injectable powder, lyophilized injectable powder, pellet, drop pill, syrup, powder,
Extractum, soft extract, oral liquid and other pharmaceutically acceptable dosage forms.
Prepare gained Herba Cistanchess extract in this patent described and can improve internal lactic acid metabolism thus playing the effect of resisting fatigue,
The purposes of the medicine, food or health product aspect of the relevant disease causing because of fatigue for preparation prevention, treatment or mitigation.
Show, the Herba Cistanchess extract of the present invention can promote lactic acid metabolism in Brachydanio rerio body, improves its motor capacity through research,
Therefore, it is expected to the Herba Cistanchess extract of the present invention or can be used for prevention, treatment containing its pharmaceutical composition or mitigate
The purposes of the medicine, food or health product aspect of the relevant disease causing because of fatigue.
Embodiment
Below by embodiment, the invention will be further described.It should be understood that embodiment of the present invention methods described
It is only used for the present invention is described, rather than limitation of the present invention, to present invention preparation side under the concept thereof of the present invention
The simple modifications of method broadly fall into the scope of protection of present invention.If no special instructions, all raw materials used in embodiment and
Solvent is purchased from Sigma Biochemical and Organic Compounds for Research and Diagnostic
Clinical Reagents company.
Embodiment 1：
By in Herba Cistanches medical material meat stem portion (and Tian Tianli sand crude drug thing Development Co., Ltd, other commercially available
Product also can) dry and pulverize, cross 60 eye mesh screens standby.Weigh 100kg Herba Cistanchess medicated powder, mix for 1: 10 in mass ratio with purified water
Close, add 200g neutral protease (enzyme activity is 300,000 u/g), adjust pH to 7～8, keeping temperature is 45～50 DEG C.Stirring carries
After taking 3h, extracting solution is boiled 5～10min, be cooled to room temperature, centrifugal filtration, filtrate is stand-by.By filtrate temperature be 55～
60 DEG C of relative densities being concentrated into when about 30 DEG C are 1.05g/mL, and spray-dried machine is dried, inlet temperature is 160～
170 DEG C, leaving air temp is 70～80 DEG C, after the completion of being dried, obtains 49kg khaki Herba Cistanchess extract (lot number is C-1).
Embodiment 2：
By in Herba Cistanches medical material meat stem portion (and Tian Tianli sand crude drug thing Development Co., Ltd, other commercially available
Product also can) dry and pulverize, cross 40 eye mesh screens standby.Weigh 100kg Herba Cistanchess medicated powder, mix for 1: 15 in mass ratio with purified water
Close, add 100g alkaline protease (enzyme activity is 400,000 u/g), adjust pH to 8～9, keeping temperature is 40～45 DEG C.Stirring carries
After taking 4h, extracting solution is boiled 5～10min, be cooled to room temperature, centrifugal filtration, filtrate is stand-by.By filtrate temperature be 55～
60 DEG C of relative densities being concentrated into when about 30 DEG C are 1.10g/mL, and vacuum dried case is dried, and temperature is 60～65 DEG C, bears
Press as 0.08～0.1MPa, after 12h is dried, obtain 56kg khaki Herba Cistanchess extract (lot number is C-2).
Embodiment 3：
By in Herba Cistanches medical material meat stem portion (and Tian Tianli sand crude drug thing Development Co., Ltd, other commercially available
Product also can) dry and pulverize, cross 40 eye mesh screens standby.Weigh 100kg Herba Cistanchess medicated powder, mix for 1: 20 in mass ratio with purified water
Close, add 150g papain (enzyme activity is 500,000 u/g), adjust pH to 7.5～8.5, keeping temperature is 50～55 DEG C.Stir
After mixing extraction 4h, extracting solution is boiled 5～10min, is cooled to room temperature, centrifugal filtration, filtrate is stand-by.Filtrate in temperature is
55～60 DEG C of relative densities being concentrated into when about 30 DEG C are 1.08g/mL, and spray-dried machine is dried, and inlet temperature is 160
～170 DEG C, leaving air temp is 70～80 DEG C, after the completion of being dried, obtains 42kg khaki Herba Cistanchess extract (lot number is C-3).
Embodiment 4：The impact evaluation to zebra fish movement for the compositionss
Laboratory animal is wild type AB strain, and each experimental group is equal 30 tail Brachydanio rerio, is incubated in 28 DEG C of incubators.Just
Often matched group is the normal Brachydanio rerio of water process of breeding fish；Induce Brachydanio rerio with sodium sulfite, in this, as Brachydanio rerio anoxia fatigue mould
Type；Take concentration to be that 2mg/mL Herba Cistanchess extract (lot number is C-1) is evaluated, be to process model group zebra with water-soluble to sample loading mode
Fish, and with ZHONGHUA DIEDA WAN as positive controls.After process terminates, the 10 tail Brachydanio rerio that each experimental group randomly chooses, measure
The total distance (S) (experimental result such as table 1) of the motion of Brachydanio rerio, the computing formula that motor capacity impact is evaluated is as follows：
Motion improvement rate (%)=[(SExtract-SModel)/(SNormally-SModel)] × 100%
Table 1 compositionss are to zebra fish movement improvement result quantitative result
| Group | Concentration (mg/mL) | Total move distance (mm, Mean ± SE) | Motion improvement result (%) |
| Normal group | / | 7635±703 | / |
| Model control group | / | 2950±202 | / |
| ZHONGHUA DIEDA WAN | 1 | 9088±447 | 131.0 |
| Extract C -1 | 2 | 8647±417 | 121.6 |
Note：Compare with model control group：P ＜ 0.001.
The total move distance of model control group Brachydanio rerio (2950mm) compares p ＜ 0.001 with Normal group (7635mm), carries
Representation model successfully constructs；Positive drug ZHONGHUA DIEDA WAN group (1mg/mL), the total move distance of Brachydanio rerio is 9088mm, with model comparison
Group compares p ＜ 0.001, and motion improvement result is 131.0%, points out the Brachydanio rerio anoxia that ZHONGHUA DIEDA WAN induces to sodium sulfite
Fatigue has extremely significant antifatigue effect.Under 2mg/mL concentration, the total move distance of Brachydanio rerio is respectively Herba Cistanchess extract：
8647mm, its motion improvement result is 121.6%, compares p ＜ 0.001 with model control group, points out Herba Cistanchess extract substantially to change
The motor capacity of the Brachydanio rerio anoxia fatigue of kind sodium sulfite induction, thus there is significant antifatigue effect.
Embodiment 4：The impact evaluation to Brachydanio rerio lactic acid metabolism for the compositionss
Laboratory animal is wild type AB strain, and each experimental group is equal 80 tail Brachydanio rerio, is incubated in 28 DEG C of incubators.Just
Often matched group is the normal Brachydanio rerio of water process of breeding fish；Induce Brachydanio rerio with sodium sulfite, in this, as Brachydanio rerio anoxia fatigue mould
Type；Take concentration to be that 2mg/mL Herba Cistanchess extract (C-1) is evaluated, be to process model group Brachydanio rerio with water-soluble to sample loading mode, and
With ZHONGHUA DIEDA WAN as positive controls.After process terminates, the lactic acid content (C) measuring in each experimental group Brachydanio rerio body is (real
Test result such as table 2), as follows to lactic acid metabolism improvement result computing formula：
Lactic acid metabolism promotion rate (%)=[(CModel-CExtract)/(CModel-CNormally)] × 100%
Table 2 compositionss are to Brachydanio rerio lactic acid metabolism facilitation quantitative result
| Group | Concentration (mg/mL) | Lactic acid content (mmol/gprot, Mean ± SE) | Lactic acid facilitation (%) |
| Normal group | / | 0.196±0.0007 | |
| Model control group | / | 0.377±0.0017 | |
| ZHONGHUA DIEDA WAN | 1 | 0.301±0.0004 | 41.9 |
| Extract C -1 | 2 | 0.335±0.0007 | 23.3 |
Note：Compare with model control group：P ＜ 0.001.
Lactic acid content (0.377mmol/gprot) and Normal group (0.196mmol/ in model control group Brachydanio rerio body
Gprot) compare p ＜ 0.001, hints model successfully constructs；Positive drug ZHONGHUA DIEDA WAN group (1mg/mL), lactic acid in Brachydanio rerio body
Content is 0.301mmol/gprot, compares p ＜ 0.001 with model control group, and its lactic acid metabolism facilitation is 41.9%, carries
Show that ZHONGHUA DIEDA WAN remarkably promotes the lactic acid metabolism in the Brachydanio rerio anoxia fatigue Brachydanio rerio body of sodium sulfite induction.Herba Cistanchess extract
Under 2mg/mL concentration, in Brachydanio rerio body, lactic acid content is 0.335mmol/gprot to thing, and lactic acid metabolism facilitation is
23.3%, compare p ＜ 0.001 with model control group, point out Herba Cistanchess extract can remarkably promote the Brachydanio rerio of sodium sulfite induction
Lactic acid metabolism in anoxia fatigue Brachydanio rerio body.
Claims (9)
1. a kind of Herba Cistanches extract is it is characterised in that be prepared by the following method：By Herba Cistanches fresh goods or dry product and water
Mixing, carries out enzyme extraction, extracting solution is concentrated into relative density 1.05～1.10g/mL when about 30 DEG C, drying is extracted
Thing.
2. Herba Cistanches extract according to claim 1 is it is characterised in that described Herba Cistanchess are optionally gone up or under ground portion, its
Middle aerial partss preferably are selected from leaf, flower, stem or seed.
3. Herba Cistanches extract according to claim 1 is it is characterised in that be prepared by the following method：Herba Cistanches meat
After matter stem portion is dried, pulverize, sieve, add water stirring, adjust temperature and pH, add bio protease to carry out enzymolysis and extraction, carry
After the completion of taking, boil, be centrifuged, filter, the concentrated drying of supernatant, obtain Herba Cistanchess extract.
4. Herba Cistanches extract according to claim 1 is it is characterised in that be prepared by the following method：
(1) the meat stem portion in Herba Cistanches medical material being dried and pulverized, crossing 40 mesh with upper screen cloth thus obtaining Herba Cistanchess medicated powder.
(2) a certain amount of Herba Cistanchess medicated powder is mixed for 1: 10～1: 20 in mass ratio with purified water, add the 0.1% of medicated powder quality
～0.5% bio protease, adjusts pH to 5～9,40～55 DEG C of stirring 3～6h of constant temperature；
(3) after the completion of extracting, the herbal extract in step (2) is boiled 5～10min, be cooled to room temperature, centrifugal filtration,
Filtrate is stand-by；
(4) filtrate in step (3) is concentrated into relative density 1.05～1.10g/mL when about 30 DEG C, drying obtains the colour of loess
Color Herba Cistanchess extract；The yield of described Herba Cistanchess extract is not less than 40%.
Preferably, described bio protease is selected from the neutral protease (enzyme activity >=200,000 u/g) of food stage, papain
(enzyme activity >=400,000 u/g), bromelain (enzyme activity >=300,000 u/g), alkaline protease (enzyme activity >=200,000 u/g), pancreas
One of enzyme (enzyme activity >=3000u/g) or their mixture, it is preferred to use neutral protease, alkaline protease,
One of papain or their mixture；
Preferably, described method for concentration can concentrate for membrance concentration, concentrating under reduced pressure, normal pressure；
Preferably, described drying meanss can be spray drying, vacuum drying, heat drying, lyophilization.
5. the extracting method of Herba Cistanches extract described in claim 1 is it is characterised in that comprise the steps：By Herba Cistanches fresh goods
Or dry product is mixed with water, carry out enzyme extraction, the relative density that extracting solution is concentrated into when about 30 DEG C is 1.05～1.10g/mL, is dried
Obtain extract.
6. extracting method according to claim 5 is it is characterised in that comprise the steps：
(1) the meat stem portion in Herba Cistanches medical material being dried and pulverized, crossing 40 mesh with upper screen cloth thus obtaining Herba Cistanchess medicated powder；
(2) a certain amount of Herba Cistanchess medicated powder is mixed for 1: 10～1: 20 in mass ratio with purified water, add the 0.1% of medicated powder quality
～0.5% bio protease, adjusts pH to 5～9,40～55 DEG C of stirring 3～6h of constant temperature；
(3) after the completion of extracting, the herbal extract in step (2) is boiled 5～10min, be cooled to room temperature, centrifugal filtration,
Filtrate is stand-by；
(4) relative density being concentrated into the filtrate in step (3) when about 30 DEG C is 1.05～1.10g/mL, and drying obtains desert
Rong's extract；The yield of described Herba Cistanchess extract is not less than 40%.
Preferably, described bio protease is selected from the neutral protease (enzyme activity >=200,000 u/g) of food stage, papain
(enzyme activity >=400,000 u/g), bromelain (enzyme activity >=300,000 u/g), alkaline protease (enzyme activity >=200,000 u/g), pancreas
One of enzyme (enzyme activity >=3000u/g) or their mixture, it is preferred to use neutral protease, alkaline protease,
One of papain or their mixture；
Preferably, described method for concentration can concentrate for membrance concentration, concentrating under reduced pressure, normal pressure；
Preferably, described drying meanss can be spray drying, vacuum drying, heat drying, lyophilization.
7. a kind of compositionss, it contains the arbitrary described extract of claim 1-4, and pharmaceutically acceptable or edible adjuvant；Can be for appointing
Meaning dosage form, preferably it is tablet, dispersible tablet, capsule, soft capsule, microcapsule, granule, injection, injectable powder, lyophilized powder
Injection, pellet, drop pill, syrup, powder, extractum, soft extract, oral liquid and other pharmaceutically can accept
Dosage form.
8. according to the arbitrary extract of claim 1-4 improving internal lactic acid metabolism thus purposes in terms of playing resisting fatigue.
9. the compositionss described in claim 7 preparation prevention, treatment or the medicine mitigating the relevant disease causing because of fatigue,
Food or the purposes of health product aspect.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233391A (en) * | 2017-07-18 | 2017-10-10 | 杏辉天力（杭州）药业有限公司 | A kind of cistanche extracts and its industrialized process for preparing and new application |
| CN107440114A (en) * | 2017-07-18 | 2017-12-08 | 杏辉天力（杭州）药业有限公司 | A kind of composition with anti-PM2.5 particulate matters effect |
| CN107586821A (en) * | 2017-10-31 | 2018-01-16 | 亿利耐雀生物科技有限公司 | A kind of extracting method and purposes of saline cistanche polypeptide |
| CN110960573A (en) * | 2019-12-24 | 2020-04-07 | 曹敏 | A fel Ursi extract, and its preparation method and application |
| CN111249338A (en) * | 2018-12-03 | 2020-06-09 | 杏辉天力（杭州）药业有限公司 | Cistanche deserticola extract and industrial preparation method and application thereof |
| CN111357976A (en) * | 2019-10-06 | 2020-07-03 | 杏辉天力（杭州）药业有限公司 | Dictyophora indusiata extract and industrial preparation method and application thereof |
| CN114515008A (en) * | 2022-03-04 | 2022-05-20 | 广东青云山药业有限公司 | Cistanche tubulosa extract and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263790A (en) * | 2014-10-17 | 2015-01-07 | 北京京隆卓尚投资有限公司 | Dsertliving cistanche polypeptide extract, as well as preparation method and use thereof |
| CN105648016A (en) * | 2016-03-30 | 2016-06-08 | 蔡庭守 | Herba cistanche small molecule peptide extract, preparation method and application thereof |
| CN105661556A (en) * | 2016-02-04 | 2016-06-15 | 中国科学院过程工程研究所 | Herba cistanche dietary fibers and preparation method and application thereof |
| CN105796656A (en) * | 2016-05-17 | 2016-07-27 | 杏辉天力（杭州）药业有限公司 | Cistanche tubulosa extract and preparation method thereof |
-
2016
- 2016-11-02 CN CN201610950842.2A patent/CN106389541A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263790A (en) * | 2014-10-17 | 2015-01-07 | 北京京隆卓尚投资有限公司 | Dsertliving cistanche polypeptide extract, as well as preparation method and use thereof |
| CN105661556A (en) * | 2016-02-04 | 2016-06-15 | 中国科学院过程工程研究所 | Herba cistanche dietary fibers and preparation method and application thereof |
| CN105648016A (en) * | 2016-03-30 | 2016-06-08 | 蔡庭守 | Herba cistanche small molecule peptide extract, preparation method and application thereof |
| CN105796656A (en) * | 2016-05-17 | 2016-07-27 | 杏辉天力（杭州）药业有限公司 | Cistanche tubulosa extract and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 丛峰松: "《神奇的小分子活性肽》", 31 May 2015, 上海：上海交通大学出版社 * |
| 庄伟: "肉从蓉提取物抗疲劳作用及其作用机制研究", 《2010年中国药学大会暨第十届中国药师周论文集》 * |
| 高建德，等: "肉苁蓉酶解提取工艺及抗氧化活性研究", 《中成药》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233391A (en) * | 2017-07-18 | 2017-10-10 | 杏辉天力（杭州）药业有限公司 | A kind of cistanche extracts and its industrialized process for preparing and new application |
| CN107440114A (en) * | 2017-07-18 | 2017-12-08 | 杏辉天力（杭州）药业有限公司 | A kind of composition with anti-PM2.5 particulate matters effect |
| CN107440114B (en) * | 2017-07-18 | 2021-02-05 | 杏辉天力（杭州）药业有限公司 | Composition with PM2.5 particle resistance effect |
| CN107586821A (en) * | 2017-10-31 | 2018-01-16 | 亿利耐雀生物科技有限公司 | A kind of extracting method and purposes of saline cistanche polypeptide |
| CN111249338A (en) * | 2018-12-03 | 2020-06-09 | 杏辉天力（杭州）药业有限公司 | Cistanche deserticola extract and industrial preparation method and application thereof |
| CN111357976A (en) * | 2019-10-06 | 2020-07-03 | 杏辉天力（杭州）药业有限公司 | Dictyophora indusiata extract and industrial preparation method and application thereof |
| CN110960573A (en) * | 2019-12-24 | 2020-04-07 | 曹敏 | A fel Ursi extract, and its preparation method and application |
| CN114515008A (en) * | 2022-03-04 | 2022-05-20 | 广东青云山药业有限公司 | Cistanche tubulosa extract and preparation method thereof |
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