CN106721005A - Tilapia mossambica prevention streptococcosis feed addictive - Google Patents
Tilapia mossambica prevention streptococcosis feed addictive Download PDFInfo
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- CN106721005A CN106721005A CN201611167690.5A CN201611167690A CN106721005A CN 106721005 A CN106721005 A CN 106721005A CN 201611167690 A CN201611167690 A CN 201611167690A CN 106721005 A CN106721005 A CN 106721005A
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- feed addictive
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- tilapia mossambica
- streptococcosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/287—Chrysanthemum, e.g. daisy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Neurosurgery (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a kind of Tilapia mossambica prevention streptococcosis feed addictive, it is prepared from by the raw material of following weight portion：20~30 parts of the root of large-flowered skullcap, 20~30 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 20~30 parts of the capsule of weeping forsythia, 0.001 0.005 parts of three isooctyl acid glyceride, 0.01 0.03 parts of starch ocentyl succinic aluminium, 20~30 parts of Radix Angelicae Sinensis.The present invention is raw materials used from pure natural medical, have the advantages that source is wide, rapid-action, have no toxic side effect, low-residual, prevention effect it is good, can effectively prevent the generation of Tilapia mossambica streptococcosis.
Description
Technical field
Added with prevention streptococcosis feed the present invention relates to a kind of fish feed additive, especially a kind of Tilapia mossambica
Agent.Belong to field of feed additive technology.
Background technology
Naturally the meat flavour of the Tilapia mossambica of growth is delicious, and fine and tender taste is either braised in soy sauce or cooked clearly, and taste is all good, Rofe
Fish contains various unrighted acids and rich in protein, is nutrition of people's raising compared with horn of plenty, and Tilapia mossambica spur is few,
There is no spinule between flesh, population all edibles are suitable for people of all ages.
The suitable Growth Op Tilapias of condition such as the natural environment and climate of China, are one of major countries of Rofe fish culture.So
And, with developing rapidly for Tilapia mossambica industry, disease causes greatly loss to Tilapia mossambica aquaculture.Wherein streptococcosis turns into
The biggest threat of Tilapia mossambica healthy aquaculture.At present, in the market is fewer for the special feed of Tilapia mossambica streptococcosis, Er Qiexiao
Fruit is mostly undesirable.Therefore, it is necessary to provide a kind of feed addictive of Tilapia mossambica with prevention streptococcosis, can effectively prevent chain
Coccus disease.So as to avoid the generation due to bacterial action in Tilapia mossambica breeding process from causing huge economic losses to raiser.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of Tilapia mossambica prevention streptococcosis feed addictive, and it can
Effectively to prevent Tilapia mossambica streptococcosis.
In order to solve the above technical problems, the present invention uses following technical scheme：Tilapia mossambica is added with prevention streptococcus feed
Agent, is prepared from by the raw material of following weight portion：It is 20~30 parts of the root of large-flowered skullcap, 20~30 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 20~30 parts of the capsule of weeping forsythia, three different pungent
Acid glyceride 0.001-0.005 parts, 0.01-0.03 parts of starch ocentyl succinic aluminium, 20~30 parts of Radix Angelicae Sinensis.
It is preferred that being made up of following raw material and its parts by weight：25 parts of the root of large-flowered skullcap, 25 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 25 parts of the capsule of weeping forsythia, three isooctyl acids
0.002 part of glyceride, 0.02 part of starch ocentyl succinic aluminium, 25 parts of Radix Angelicae Sinensis.
It is preferred that being made up of following raw material and its parts by weight：30 parts of the root of large-flowered skullcap, 20 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 20 parts of the capsule of weeping forsythia, three isooctyl acids
0.002 part of glyceride, 0.02 part of starch ocentyl succinic aluminium, 30 parts of Radix Angelicae Sinensis.
Above-mentioned feed addictive, raw material is crushed and is clayed into power, and is extracted using decoction alcohol precipitation method, then rotated evaporation is extracted
Obtain.
Decoction alcohol precipitation method is comprised the following steps：
1st, the root of large-flowered skullcap, FLOS CHRYSANTHEMI ALBA from Haizhou of China, the capsule of weeping forsythia, three isooctyl acid glyceride, starch ocentyl succinic aluminium, Radix Angelicae Sinensis are weighed by weight,
By mass volume ratio 1:4 soak 0.5h, 1h water temperatures are heated afterwards and is maintained at 100 DEG C, filtering；
2nd, filter residue presses mass volume ratio 1 again:4 add water, and directly heat 1h, and temperature is still maintained at 100 DEG C, and filtering is repeated twice；
3rd, the filtrate that will be obtained merges, and is placed on and 1h is centrifuged on the centrifuge of 3000r/min；Supernatant is taken again in Rotary Evaporators
Upper extraction, adds 95% alcohol, makes mixed liquor concentration of alcohol up to 80%, stands 24h, and rotary evaporation recovered alcohol to liquor strength is
1g/mL；115 DEG C of autoclaving 20min, -20 DEG C save backup.
The application method of above-mentioned feed addictive, admixes additive basal feed and throws by 50~150mL liquids/kg feeds
Hello.
Lack the problem of safe and effective medicine for Tilapia mossambica streptococcosis, the present invention is made of pure Chinese medicine, and formula is closed
Reason, green is pure natural, and validity is high.It is of the invention raw materials used most of from pure natural medical, with wide, action of originating
The advantages of hurry up, have no toxic side effect, reducing illness, low-residual, good prevention effect.The experiment proved that, in the time pin of susceptible disease
To the use additive of the invention of property, can effectively prevent the generation of Tilapia mossambica streptococcosis.
Specific embodiment
Embodiment 1
The Tilapia mossambica preparation for preventing streptococcosis feed addictive
The Tilapia mossambica of table 1 prevention streptococcosis feed addictive prescription, is prepared from by the raw material of following weight portion：
| Sequence number | The root of large-flowered skullcap | FLOS CHRYSANTHEMI ALBA from Haizhou of China | The capsule of weeping forsythia | Radix Angelicae Sinensis | Three isooctyl acid glyceride | Starch ocentyl succinic aluminium |
| 1 | 20 | 30 | 20 | 30 | 0.002 | 0.02 |
| 2 | 25 | 25 | 25 | 25 | 0.002 | 0.02 |
| 3 | 20 | 30 | 30 | 20 | 0.001 | 0.01 |
| 4 | 25 | 30 | 20 | 25 | 0.002 | 0.02 |
| 5 | 20 | 25 | 30 | 25 | 0.002 | 0.02 |
The formula of reference table 1, takes the additive that herbal raw material prepares formula one to five respectively, first crushes herbal raw material
Clay into power, extracted using decoction alcohol precipitation method, then rotated evaporation is extracted and obtained, it is specific as follows：
1st, the root of large-flowered skullcap, FLOS CHRYSANTHEMI ALBA from Haizhou of China, the capsule of weeping forsythia, three isooctyl acid glyceride, starch ocentyl succinic aluminium, Radix Angelicae Sinensis are weighed by weight,
By mass volume ratio 1:4 soak 0.5h, 1h water temperatures are heated afterwards and is maintained at 100 DEG C, filtering；
2nd, filter residue presses mass volume ratio 1 again:4 add water, and directly heat 1h, and temperature is still maintained at 100 DEG C, and filtering is repeated twice；
3rd, the filtrate that will be obtained merges, and is placed on and 1h is centrifuged on the centrifuge of 3000r/min；Supernatant is taken again in Rotary Evaporators
Upper extraction, adds 95% alcohol, makes mixed liquor concentration of alcohol up to 80%, stands 24h, and rotary evaporation recovered alcohol to liquor strength is
1g/mL；115 DEG C of autoclaving 20min, -20 DEG C save backup.
The application method of above-mentioned feed addictive, admixes additive basal feed and throws by 50~150mL liquids/kg feeds
Hello.A small amount of three isooctyl acid glyceride, starch ocentyl succinic aluminium composition, can be combined with the plant component material such as Radix Angelicae Sinensis,
Stimulation can be formed in fish body, enhance immunity, anti-inflammation, reduction are ill.
Application method：In hammer bacterial diseases season occurred frequently using being prevented epidemic, to prevent breaking out for streptococcus disease.Will
The liquid of formula 1 to 5 presses liquid feed ratio 50,75,100,125,150mL/kg respectively, is uniformly sprayed additive with sprinkler
In on the daily conventional tilapia feed of respective quality, stir and evenly mix, it is fully merged with feed, the 24h that dries in the shade is standby.To contain
The feed of additive is daily to be fed by 5% amount of body weight, continuous to use 10 days.As a result：The death rate reaches after infection control group morbidity
To 95.83%, the control drug group Florfenicol treatment group death rate is 70.83%, and protective rate is 26.08%.Compound Chinese herb feed
Additive preventive effect is notable, high dose group（100ml/kg）, middle dose group（50ml/kg）, low dose group（25ml/kg）Guarantor
Shield rate is respectively 47.82%, 34.78%, 26.08%, obviously higher than Florfenicol treatment group and infection control group.
The Tilapia mossambica of present invention research prevention streptococcosis feed addictive is to Tilapia mossambica streptococcosis preventive effect
Research
1 MATERIALS METHODS
1.1 materials
(1) bacterium is used in experiment
Streptococcusagalactiae is purchased from Oceanography Institute Of Zhejiang.Strain streak inoculation is in sheep blood plate culture medium, 37 DEG C of 16~8h of culture.
Picking single bacterium colony, is seeded in 5% serum brain heart oxoid broth bouillon, 37 DEG C of cultures to 16~18h.Given birth to when using
Reason 10 times of dilutions of salt solution are standby.
(2) experimental drug
Compound Chinese herbal medicinal feed additive（Prepared by the formula of embodiment 12）.
Florfenicol, purchased from Beijing Hua Bang animal pharmaceutical estates Co., Ltd
(3) Experimental fish
Selected from the gift tilapia of certain plant（Body weight 50~100g/ bodies 8~15cm long）
1.2 methods
（1）The preparation of feed addictive
Preparation method is with embodiment 1.
Liquid is pressed into certain liquid feed ratio respectively（mL/kg）, additive is uniformly sprayed on respective quality with sprinkler
Daily conventional tilapia feed on, stir and evenly mix, it is fully merged with feed, the 24h that dries in the shade is standby.Florfenicol addition
It is 0.05g/kg.
（2）Experimental fish prepares
Selected from the gift tilapia of certain plant（Body weight 50~100g/ bodies 8~15cm long）, temporarily supported in pool before experiment.
（3）Bacterium is used in experiment
Strain is taken before experiment, line is pure to carry out pure culture in blood plate.37 DEG C of 16~18h of culture.Again lower bacterium is washed with physiology salt
Fall, it is standby to be configured to 7.5 × 108CFU/mL bacterium solutions using turbidimetry.
（4）Experiment process
The tail of Tilapia mossambica 300 is taken, is divided into infection not administration group, low dose group（25ml/kg）, middle dose group（50ml/kg）, high dose
Group（150ml/kg）, Florfenicol control group and saline control group.Infection control group and saline control group are only given
Conventional feed, remaining each group feeds corresponding pastille feed.After feeding 10 days abdominal cavity note is carried out with Streptococcusagalactiae clinical separation strain
Penetrate and attack poison, toadstool amount is attacked for 7.5 × 108 CFU/mL, per tail 0.2mL.Observed 10 days after attacking poison, statistics Tilapia mossambica death feelings
Condition, and the illness change of Tilapia mossambica after poison is attacked in observation.Dead Tilapia mossambica takes brain tissue to be inoculated in blood plate in 37 DEG C of cultures, sees
Examine and whether there is streptococcus growth.Protective rate of the prescription to Tilapia mossambica is calculated by each group fish death toll.Protective rate computational methods：Protective rate
=（Positive infection group death toll-test group death toll）/ positive infection group death toll × 100%.
2 experimental results
Attack 7 days each group death conditions statistics such as table 2 after poison：
The experimental result of table 2
| Date | 25ml/kg | 50ml/kg | 100ml/kg | Florfenicol | Empty hundred compare | Negative control |
| Sum | 50 | 50 | 50 | 50 | 50 | 50 |
| Death toll | 22 | 15 | 10 | 22 | 49 | 0 |
| It is remaining | 28 | 35 | 40 | 28 | 1 | 50 |
| The death rate | 44% | 30% | 20% | 44% | 2% | 0 |
| Protective rate | 56% | 70% | 80% | 56% |
Take dead fish brain tissue to be inoculated with blood plate, find there is streptococcus growth through culture.Do not find afterwards within 10th day
Dead fish, Tilapia mossambica is all put to death for survival, takes brain tissue to be inoculated in blood plate, there are no streptococcus generation.
3 experiment conclusions
Feed addictive of the invention can effectively prevent the generation of Tilapia mossambica streptococcosis, achieve expected result, be worth into one
Step is developed.
Claims (6)
1. a kind of Tilapia mossambica is used and prevents streptococcosis feed addictive, it is characterised in that be made up of by weight following raw material：It is yellow
20~30 parts of a kind of reed mentioned in ancient books, 20~30 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 20~30 parts of the capsule of weeping forsythia, 0.001-0.005 parts of three isooctyl acid glyceride, starch octene
0.01-0.03 parts of base butanedioic acid aluminium, 20~30 parts of Radix Angelicae Sinensis.
2. feed addictive according to claim 1, it is characterised in that be preferably prepared from by the raw material of following weight portion：
25 parts of the root of large-flowered skullcap, 25 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 25 parts of the capsule of weeping forsythia, 0.002 part of three isooctyl acid glyceride, 0.02 part of starch ocentyl succinic aluminium,
25 parts of Radix Angelicae Sinensis.
3. feed addictive according to claim 1, it is characterised in that be preferably prepared from by the raw material of following weight portion：
30 parts of the root of large-flowered skullcap, 20 parts of FLOS CHRYSANTHEMI ALBA from Haizhou of China, 20 parts of the capsule of weeping forsythia, 0.002 part of three isooctyl acid glyceride, 0.02 part of starch ocentyl succinic aluminium,
30 parts of Radix Angelicae Sinensis.
4. feed addictive according to claim 1, it is characterised in that：Above-mentioned feed addictive, raw material is crushed and is worn into
Powder, is extracted using decoction alcohol precipitation method, then rotated evaporation is extracted and obtained.
5. feed addictive according to claim 1, it is characterised in that the decoction alcohol precipitation method is comprised the following steps：
1）The root of large-flowered skullcap, FLOS CHRYSANTHEMI ALBA from Haizhou of China, the capsule of weeping forsythia, three isooctyl acid glyceride, starch ocentyl succinic aluminium, Radix Angelicae Sinensis are weighed by weight,
By mass volume ratio 1:4 soak 0.5h, 1h water temperatures are heated afterwards and is maintained at 100 DEG C, filtering；
2）Filter residue presses mass volume ratio 1 again:4 add water, and directly heat 1h, and temperature is still maintained at 100 DEG C, and filtering is repeated twice；
3）The filtrate that will be obtained merges, and is placed on and 1h is centrifuged on the centrifuge of 3000r/min；Supernatant is taken again in Rotary Evaporators
Upper extraction, adds 95% alcohol, makes mixed liquor concentration of alcohol up to 80%, stands 24h, and rotary evaporation recovered alcohol to liquor strength is
1g/mL；115 DEG C of autoclaving 20min, -20 DEG C save backup.
6. the application method of feed addictive described in claim 1, it is characterised in that：Additive is pressed into 50~150mL liquids/kg
Feed is admixed basal feed and is fed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611167690.5A CN106721005A (en) | 2016-12-16 | 2016-12-16 | Tilapia mossambica prevention streptococcosis feed addictive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611167690.5A CN106721005A (en) | 2016-12-16 | 2016-12-16 | Tilapia mossambica prevention streptococcosis feed addictive |
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| Publication Number | Publication Date |
|---|---|
| CN106721005A true CN106721005A (en) | 2017-05-31 |
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ID=58893197
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| CN201611167690.5A Withdrawn CN106721005A (en) | 2016-12-16 | 2016-12-16 | Tilapia mossambica prevention streptococcosis feed addictive |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041274A (en) * | 2017-11-29 | 2018-05-18 | 杨韶彰 | Prevent the compound Chinese herbal medicinal feed additive of Tilapia mossambica streptococcosis |
| CN108186621A (en) * | 2018-01-19 | 2018-06-22 | 海南大学 | Purposes of the succinic acid in aquatic livestock streptococcosis protective agents are prepared |
| CN108420922A (en) * | 2018-04-12 | 2018-08-21 | 广西大学 | Anti- Tilapia mossambica streptococcosis Chinese herbal and crude drugs preparations and its preparation process |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763782A (en) * | 2012-08-01 | 2012-11-07 | 中国水产科学研究院珠江水产研究所 | Compound Chinese herbal medicine preparation for improving tilapia immunity |
| CN103844143A (en) * | 2014-03-11 | 2014-06-11 | 广西大学 | Chinese herbal compound feed additive preventing streptococcicosis for tilapia mossambica |
-
2016
- 2016-12-16 CN CN201611167690.5A patent/CN106721005A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763782A (en) * | 2012-08-01 | 2012-11-07 | 中国水产科学研究院珠江水产研究所 | Compound Chinese herbal medicine preparation for improving tilapia immunity |
| CN103844143A (en) * | 2014-03-11 | 2014-06-11 | 广西大学 | Chinese herbal compound feed additive preventing streptococcicosis for tilapia mossambica |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041274A (en) * | 2017-11-29 | 2018-05-18 | 杨韶彰 | Prevent the compound Chinese herbal medicinal feed additive of Tilapia mossambica streptococcosis |
| CN108186621A (en) * | 2018-01-19 | 2018-06-22 | 海南大学 | Purposes of the succinic acid in aquatic livestock streptococcosis protective agents are prepared |
| CN108420922A (en) * | 2018-04-12 | 2018-08-21 | 广西大学 | Anti- Tilapia mossambica streptococcosis Chinese herbal and crude drugs preparations and its preparation process |
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Application publication date: 20170531 |