CN102763782A - Compound Chinese herbal medicine preparation for improving tilapia immunity - Google Patents
Compound Chinese herbal medicine preparation for improving tilapia immunity Download PDFInfo
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- CN102763782A CN102763782A CN2012102708186A CN201210270818A CN102763782A CN 102763782 A CN102763782 A CN 102763782A CN 2012102708186 A CN2012102708186 A CN 2012102708186A CN 201210270818 A CN201210270818 A CN 201210270818A CN 102763782 A CN102763782 A CN 102763782A
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Abstract
A compound Chinese herbal medicine preparation for improving tilapia immunity is obtained by mixing the following components, by weight, 40% of houttuynia cordata, 5% of radix scutellariae, 5% of isatis root, 5% of fructus forsythiae, 10% of honeysuckle, 5% of liquorice, 5% of rheum officinale, 10% of astragalus, 5% of angelica sinensis and 10% of hawthorn. The compound Chinese herbal medicine preparation can remarkably improve activity of superoxide dismutase (SOD) in tilapia serum, the content of nitric oxide (NO), the activity of serum lysoyme (LSZ), phagocytic percentage, phagocytic index and respiratory burst, greatly reduces the content of serum malondialdehyde (MDA) of tilapia, and effectively improves the tilapia immunity. Feed containing the compound Chinese herbal medicine preparation is further disclosed. The production process is simple, the compound Chinese herbal medicine preparation can be added into commodity feed directly and can not affect taste of the feed, Chinese herbal medicine raw materials used in the preparation are low in price, wide and stable in sources, and the preparation can be produced in industrialization mode.
Description
Technical field
The present invention relates to Chinese herbal feed additive, specifically can improve the compound Chinese herbal medicine additive of Tilapia mossambica immunity.
Background technology
Tilapia mossambica is that one of fish of culturing are recommended by FAO (Food and Agriculture Organization of the United Nation) to countries in the world; It is assorted to have feeding habits; Characteristics such as growth is fast, and population yield is high, and adaptability is strong; Extensively culture in south China tropical and subtropical zone area in recent years, become the breed variety that China has competitiveness in the international market most at present.Yet, being accompanied by large-area breed, various disease problems also become increasingly conspicuous, and especially streptococcus is caused serious threat to China's Tilapia mossambica industry especially.Usually use antibiotic medicine to be used for treating streptococcosis in the production, but a large amount of antibiotic abuses can bring medicine residual again, cause more serious food-safety problem.Therefore, the disease problem has become restriction Tilapia mossambica industry and has further developed, influenced one of principal element of product quality.Discover that in feed, adding some Chinese herbal medicines can improve the autoimmune power of fish body, builds up one's resistance to disease.
Chinese herbal medicine is remote in the use history of China, mainly is because advantages such as it is efficient, low toxicity, low-residual, and contains panimmunity and regulate active component such as alkaloid, polysaccharide, saponin, anthracene class, volatile oil, organic acid etc.Some scholar's research the influence of Chinese herbal medicine to fish immunity function and diseases prevention anti-stress ability.Oleuropein as from olive growing leaves, extracting can effectively suppress salmon rhabdovirus and viral hemorrhagic property leukemia virus.Radix-polygoni multiflori extract (PME) Wheat Protein helps the removing of interior free yl.In feed, add the Turmeric P.E rainbow trout of feeding and significantly to increase cytophagous phagocytosis in the blood.Nutrient substances such as these organic acids that contain owing to Chinese herbal medicine in addition, tannin, alkaloid, flavones, glycoside, inorganic salts can impel the synthetic of organism metabolism and protein, thereby accelerate the speed of growth and improve meat quality.Rate of body weight gain, specific growth rate, the protein content of Luo Fei juvenile fish be can effectively improve as in basal feed, adding more than 20 kind of Chinese herbal medicines such as the Radix Astragali, honeysuckle, kuh-seng, Chinese violet, feed coefficient and crude fat content reduced.Therefore, the research Chinese herbal medicine is to the effect of Tilapia mossambica immunity, and the Chinese herbal medicine immunopotentiator of developing efficient, safety, environmental protection is significant to the sound development of Tilapia mossambica industry.
Summary of the invention
The purpose of this invention is to provide a kind of Chinese herbal formula that improves Tilapia mossambica immunity, this Chinese herbal formula can effectively improve the immunity of Tilapia mossambica, reduces the generation of Tilapia mossambica disease.
The present invention also aims to provide and contain the above-mentioned tilapia feed that can improve the Chinese herbal formula of Tilapia mossambica immunity; This feed production technology is simple and convenient, and this tilapia feed that contains Chinese herbal formula can effectively improve the immunity of Tilapia mossambica through throwing something and feeding.
For realizing first purpose of the present invention, a kind of Chinese herbal formula that improves Tilapia mossambica immunity provided by the invention obtains through following component and weight proportion mixing:
Cordate houttuynia (40%), the root of large-flowered skullcap (5%), Radix Isatidis (5%), the capsule of weeping forsythia (5%), honeysuckle (10%), Radix Glycyrrhizae (5%), rheum officinale (5%), the Radix Astragali (10%), Radix Angelicae Sinensis (5%), hawthorn (10%).
The preparation method of the Chinese herbal formula of above-mentioned raising Tilapia mossambica immunity is: earlier the ultra micro powder is processed in each component oven dry, mixed by above-mentioned weight proportion.
For realizing second purpose of the present invention; The present invention provides and contains the above-mentioned tilapia feed that can improve the Chinese herbal formula of Tilapia mossambica immunity; Above-mentioned each component raw material is processed the ultra micro powder through oven dry; Mix Chinese herbal formula by above-mentioned weight proportion again, Chinese herbal formula by weight percentage for 3-6% joins in the tilapia feed, is processed a material feed of respective diameters size behind the mixing.
Advantage of the present invention is: Chinese herbal formula of the present invention can significantly improve superoxide dismutase in the Tilapia mossambica serum (SOD) vigor, nitric oxide (NO) content, serum lysozyme (LSZ) vigor, percentage phagocytosis, phagocytic index and respiratory burst vigor; Significantly reduce Tilapia mossambica serum MDA (MDA) content; Effectively improve the immunity of Tilapia mossambica, reduce the generation of Tilapia mossambica disease.Contain the feed of this Chinese herbal formula, production technology is simple, can directly add in the commercial feed, can the palatability of feed not exerted an influence, and used herbal raw material is cheap, and wide material sources are stable, are easy to industrialization production.
The specific embodiment
1, the Chinese herbal formula that provides of this instance, mix to obtain through following component and weight proportion:
Cordate houttuynia (40%), the root of large-flowered skullcap (5%), Radix Isatidis (5%), the capsule of weeping forsythia (5%), honeysuckle (10%), Radix Glycyrrhizae (5%), rheum officinale (5%), the Radix Astragali (10%), Radix Angelicae Sinensis (5%), hawthorn (10%).
Its preparation method is: earlier the ultra micro powder is processed in each component oven dry, mixed Chinese herbal formula by above-mentioned weight proportion again.
2, the present invention provides the tilapia feed that contains the Chinese herbal formula that can improve Tilapia mossambica immunity; By the method in the instance 1 each component raw material is processed the ultra micro powder through oven dry; Mixing Chinese herbal formula by above-mentioned weight proportion again, is that 0 (control group), 1% (A group), 3% (B group), 6% (C group) add in the basic tilapia feed with Chinese herbal formula by mass fraction, and processing diameter behind the mixing is the material feed of 3-5mm; Use oven for drying, process 4 kinds of tests and use feed.
3, experimental design and feeding and management: select neat specification, physique Tilapia mossambica healthy and strong, that the average body quality is 54 ± 2.32g and be divided into 4 groups at random; Put into 12 cement pits (470cm * 270cm * 80cm); Every pond 70 tails; The above-mentioned 4 kinds of test feeds of throwing something and feeding are respectively established 3 repetitions for every group, and test was carried out for 12 weeks continuously.Test water is for through the running water of aeration, and with inflator pump oxygenation, a day feeding volume is a fish body weight 3%, in the morning every day 9:00 with afternoon 5:00 respectively throw something and feed half.Begin formal test after raising and train 14d, the duration of test water temperature is (29 ± 2) ℃, and duration of test water body DO remains on more than the 5mg/L, pH value 7.5 ~ 8.0, and regularly add and change water.
4, above-mentioned the stating during the feed of throwing something and feeding respectively at each sampling of the 4th, 8,12 weeks once, measured Tilapia mossambica haematogenic immunity index.Get 12 tails (every case 4 tails) at random, take a blood sample in tail vein place for each every group with syringe.Only gather the mensuration that serum carries out the relevant enzyme vigor preceding twice.Anticoagulation and non-anticoagulation are collected in blood sampling at twice respectively for the last time.A part is with aseptic liquaemin anti-freezing, and the blood of collection is whole blood, is used for measuring the respiratory burst vigor of cytophagous phagocytic activity of blood and haemocyte.Go to rapidly after the non-anticoagulation collection in the aseptic centrifuge tube of 2ml; Leave standstill under the room temperature treat its blood coagulation after; 4 ℃ of 3000r/min are centrifugal; Collect serum and be stored in-80 ℃ of ultra low temperature freezers, be used for measuring serum superoxide dismutase (SOD), lysozyme (LSZ), nitric oxide (NO), MDA enzyme activity and content such as (MDA).
5, result of the test shows: since the 8th week; B group bolti serum MDA (MDA) content reduces very significantly (P＜0.01); And bolti serum lysozyme (LSZ) vigor significantly improves (P＜0.05) in the C group; Superoxide dismutase (SOD) vigor, nitric oxide (NO) content improve very significantly (P＜0.01), and MDA content reduces (P＜0.01) extremely significantly.To the 12nd week, the active utmost point of bolti serum activity of SOD and haemocyte respiratory burst significantly improves (P＜0.01) in the B group, and serum LSZ vigor, NO content significantly improve (P＜0.05); In the C group bolti serum activity of SOD, NO content, serum LSZ vigor, percentage phagocytosis, phagocytic index, respiratory burst vigor all the utmost point significantly improve (P＜0.01), MDA content then reduces very significantly (P＜0.01).The result shows: what in feed, add 3% and 6% mass fraction is that the Chinese herbal formula of main component all can strengthen the immunity of bolti with the cordate houttuynia, wherein best with the effect of adding 6% mass fraction.
6, above-mentioned embodiment explains that compound Chinese herbal medicine additive provided by the invention can effectively improve Tilapia mossambica immunity, strengthens the ability that body is removed foreign matter, thereby improves the premunition of Tilapia mossambica.
Claims (3)
1. Chinese herbal formula that improves Tilapia mossambica immunity; The drug ingedient that it is characterized in that it is pure Chinese herbal medicine; Various medicinal herb componentses are respectively: cordate houttuynia (40%), the root of large-flowered skullcap (5%), Radix Isatidis (5%), the capsule of weeping forsythia (5%), honeysuckle (10%), Radix Glycyrrhizae (5%), rheum officinale (5%), the Radix Astragali (10%), Radix Angelicae Sinensis (5%), hawthorn (10%); Each component of Chinese herbal medicine is processed the ultra micro powder earlier, mixes by above-mentioned mass fraction proportioning again.
2. tilapia feed that improves the Chinese herbal formula of Tilapia mossambica immunity is characterized in that improving the Chinese herbal formula 3%-6% of Tilapia mossambica immunity;
Basis tilapia feed 94%-97% is with the ultra micro powder of the Chinese herbal formula that improves Tilapia mossambica immunity, is mixed in the basic tilapia feed by above-mentioned weight proportion again, processes the pellet that diameter is 3-5mm behind the mixing, puts in storage after the drying.
3. according to the said tilapia feed that improves the Chinese herbal formula of Tilapia mossambica immunity of claim 2, it is characterized in that improving the Chinese herbal formula 6% of Tilapia mossambica immunity;
Basis tilapia feed 94% is with the ultra micro powder of the Chinese herbal formula that improves Tilapia mossambica immunity, is mixed in the basic tilapia feed by above-mentioned weight proportion again, processes the pellet that diameter is 3-5mm behind the mixing, puts in storage after the drying.
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| CN103071121A (en) * | 2013-02-01 | 2013-05-01 | 中国水产科学研究院黑龙江水产研究所 | Chinese medicinal herb immunopotentiator for oncorhynchus masou masou |
| CN103250875A (en) * | 2013-06-08 | 2013-08-21 | 何兴江 | Special compound feed for tilapia |
| CN103262961A (en) * | 2013-06-08 | 2013-08-28 | 何兴江 | Feed additive specially used for tilapia |
| CN103355482A (en) * | 2012-06-04 | 2013-10-23 | 青岛玛斯特生物技术有限公司 | Compound Chinese herbal medicine feed additive for allogyogenetics silver crucian carp |
| CN103392940A (en) * | 2013-08-06 | 2013-11-20 | 中国水产科学研究院淡水渔业研究中心 | Feed for improving streptococcal disease resistance of tilapia and feeding method thereof |
| CN103583810A (en) * | 2013-11-26 | 2014-02-19 | 青岛嘉瑞生物技术有限公司 | Fermentation type Chinese herbal medicinal immunopotentiator for erythroculter ilishaeformis |
| CN103598485A (en) * | 2013-12-06 | 2014-02-26 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of Chinese herbal immunopotentiator used for snakeheads |
| CN103704519A (en) * | 2013-12-24 | 2014-04-09 | 广东粤海饲料集团有限公司 | Chinese herbal anti-disease feed additive for prawns and preparation method thereof |
| CN103734537A (en) * | 2014-01-09 | 2014-04-23 | 中国水产科学研究院淡水渔业研究中心 | Method for improving streptococcus iniae infection-resisting capacity of GIFT oreochromis niloticus during high-temperature stress |
| CN104222551A (en) * | 2014-08-08 | 2014-12-24 | 广东海大集团股份有限公司 | Natural feed additive for preventing and treating body surface ulcer of jewfish |
| CN104286598A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Feed for tilapia in breeding season |
| CN104431628A (en) * | 2014-12-30 | 2015-03-25 | 张嘉强 | Mixed feed for red tilapias |
| CN104431368A (en) * | 2014-12-15 | 2015-03-25 | 中国水产科学研究院长江水产研究所 | Compound feed additive for promoting healthy growth of fishes and preparation method |
| CN104543512A (en) * | 2014-12-24 | 2015-04-29 | 武汉华扬动物药业有限责任公司 | Traditional Chinese medicine feed additive improving quality of fresh water aquiculture animal and preparation method for traditional Chinese medicine feed additive |
| CN105166491A (en) * | 2015-09-03 | 2015-12-23 | 哈尔滨泉兴科技有限公司 | Tilapia feed and preparation method therefor |
| CN105341352A (en) * | 2015-12-15 | 2016-02-24 | 李国深 | Feed additive for preventing streptococcus agalactiae |
| CN105393955A (en) * | 2015-10-29 | 2016-03-16 | 苏州市美人半岛齐力生态农产品专业合作社 | High-yield culture method for Oreochromismossambicus |
| CN105995205A (en) * | 2016-05-27 | 2016-10-12 | 怀宁县龙盛草业有限公司 | Feed capable of improving immunity of carps and preparation method of feed |
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| CN108077123A (en) * | 2017-12-26 | 2018-05-29 | 广西金海环岛渔业有限公司 | Improve the cultural method of Tilapia mossambica immunity |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103355482A (en) * | 2012-06-04 | 2013-10-23 | 青岛玛斯特生物技术有限公司 | Compound Chinese herbal medicine feed additive for allogyogenetics silver crucian carp |
| CN103071121A (en) * | 2013-02-01 | 2013-05-01 | 中国水产科学研究院黑龙江水产研究所 | Chinese medicinal herb immunopotentiator for oncorhynchus masou masou |
| CN103250875A (en) * | 2013-06-08 | 2013-08-21 | 何兴江 | Special compound feed for tilapia |
| CN103262961A (en) * | 2013-06-08 | 2013-08-28 | 何兴江 | Feed additive specially used for tilapia |
| CN103250875B (en) * | 2013-06-08 | 2014-03-19 | 何兴江 | Special compound feed for tilapia |
| CN103392940A (en) * | 2013-08-06 | 2013-11-20 | 中国水产科学研究院淡水渔业研究中心 | Feed for improving streptococcal disease resistance of tilapia and feeding method thereof |
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| CN103583810A (en) * | 2013-11-26 | 2014-02-19 | 青岛嘉瑞生物技术有限公司 | Fermentation type Chinese herbal medicinal immunopotentiator for erythroculter ilishaeformis |
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| CN104222551A (en) * | 2014-08-08 | 2014-12-24 | 广东海大集团股份有限公司 | Natural feed additive for preventing and treating body surface ulcer of jewfish |
| CN104286598A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Feed for tilapia in breeding season |
| CN104431368A (en) * | 2014-12-15 | 2015-03-25 | 中国水产科学研究院长江水产研究所 | Compound feed additive for promoting healthy growth of fishes and preparation method |
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| CN104431628A (en) * | 2014-12-30 | 2015-03-25 | 张嘉强 | Mixed feed for red tilapias |
| CN105166491A (en) * | 2015-09-03 | 2015-12-23 | 哈尔滨泉兴科技有限公司 | Tilapia feed and preparation method therefor |
| CN105393955A (en) * | 2015-10-29 | 2016-03-16 | 苏州市美人半岛齐力生态农产品专业合作社 | High-yield culture method for Oreochromismossambicus |
| CN105341352A (en) * | 2015-12-15 | 2016-02-24 | 李国深 | Feed additive for preventing streptococcus agalactiae |
| CN105995205A (en) * | 2016-05-27 | 2016-10-12 | 怀宁县龙盛草业有限公司 | Feed capable of improving immunity of carps and preparation method of feed |
| CN106172144B (en) * | 2016-09-13 | 2019-04-16 | 中国水产科学研究院淡水渔业研究中心 | A method of promoting gift tilapia seed uniform growth and immunologic facilitation |
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| CN106721005A (en) * | 2016-12-16 | 2017-05-31 | 新昌县柏克动物饲料技术开发有限公司 | Tilapia mossambica prevention streptococcosis feed addictive |
| CN108077123A (en) * | 2017-12-26 | 2018-05-29 | 广西金海环岛渔业有限公司 | Improve the cultural method of Tilapia mossambica immunity |
| CN108478637A (en) * | 2018-03-28 | 2018-09-04 | 天津农学院 | White point salmon raising Chinese medicine composition and application method |
| CN108653557A (en) * | 2018-03-28 | 2018-10-16 | 天津农学院 | Yadong salmon raising Chinese medicine composition and application method |
| CN108835385A (en) * | 2018-08-01 | 2018-11-20 | 中国海洋大学 | A kind of prawn compound Chinese herb removing toxic substances liver protection feed addictive |
| CN108835385B (en) * | 2018-08-01 | 2023-12-19 | 中国海洋大学 | Compound Chinese herbal medicine feed additive for detoxifying and protecting liver of prawns |
| WO2021203688A1 (en) * | 2020-04-10 | 2021-10-14 | 江苏省淡水水产研究所 | Liver and intestine protecting immune-potentiating composition for freshwater fish, preparation method therefor, and application thereof |
| CN111513209A (en) * | 2020-04-30 | 2020-08-11 | 中国热带农业科学院热带生物技术研究所 | TiLV (TiLV-resistant Tilapia mossambica) feed additive and application |
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