CN102068638B - Preparation method and application of composite Indian buead preparation - Google Patents
Preparation method and application of composite Indian buead preparation Download PDFInfo
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- CN102068638B CN102068638B CN2011100078074A CN201110007807A CN102068638B CN 102068638 B CN102068638 B CN 102068638B CN 2011100078074 A CN2011100078074 A CN 2011100078074A CN 201110007807 A CN201110007807 A CN 201110007807A CN 102068638 B CN102068638 B CN 102068638B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the technical field of research and development of medical products and relates to a preparation method and application of a composite Indian buead preparation. The method comprises the following steps of: selecting 6 to 9 grams of high-quality Indian buead, 6 to 9 grams of agaric, 6 to 9 grams of tangerine peel, 6 to 9 grams of Chinese angelica, 6 to 9 grams of tuber fleeceflower root, 6 to 9 grams of American ginseng, 6 to 9 grams of acanthopanax root, 4 to 6 grams of manyflower solomonseal rhizome, 4 to 6 grams of yam, 4 to 6 grams of hawthorn, 2 to 4 grams of malt and 2 to 4 grams of Chinese date; washing; performing microwave drying; smashing; adding water for boiling and extracting for three times; combining extracting solution; filtering; condensing filtrate into extract; uniformly mixing the extract and starch in the ratio of 1:4; and pelletizing so as to obtain the composite Indian buead preparation. A product can enhance the oxidation resistance of superoxide dismutase in liver of fancy carp and lower the malondialdehyde content of the liver. The product is beneficial to industrial production.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of preparation method and its usage of compound poria cocos preparation.Product of the present invention has the oxidation resistance that can improve superoxide dismutase in the Cyprinus carpio L. liver and reduces MDA content in the liver, and product of the present invention is beneficial to suitability for industrialized production.
Background technology
Advantage such as that Chinese herbal feed additive has is natural, economic, efficient, noresidue, no Drug resistance and side effect are little, and have the dual function of medical and trophism concurrently, the drawback of no antibiotics and chemicals, be widely used in recent years in the aquaculture of aquatic animal, in order to prevent disease, growth promotion with improve aquatic product quality.Contain multiple nutritional components and bioactive substances such as polysaccharide, aminoacid, protein, organic acid, tannin, alkaloid, flavone, glycoside, inorganic salt in the Chinese traditional compound medicine, can promote the synthetic of animal body metabolism and protein and enzyme, the scalable immunity function of animal is accelerated the speed of growth.Therefore mainly can to improve the animal body immunologic function relevant with it to it is generally acknowledged Chinese herbal medicine prevent disease and growth promoting function.Superoxide dismutase (SOD) can impel peroxide radical to change into hydrogen peroxide and oxygen, thus the peroxide radical of supervening in the eliminate the inflammation process, and powerful antiinflammatory action is arranged.Malonaldehyde (MDA) is the final catabolite of lipid peroxidation, its content can reflect the levels of peroxide of the reactive oxygen free radical and the lipid of body indirectly, thereby the degree that reflects the cell damaged has indirectly recently begun to be applied to the research of Aquatic Ecotoxicology.The Cyprinus carpio L. body body-building, beautiful in colour, be a kind of integrate view and admire, fish, the large-scale famous and precious Fish of cuisines, have the title of " funny fellow's stone in the water ".The ornamental value of Cyprinus carpio L. mainly is body colour that it is bright-coloured and colourful figure.Simultaneously Cyprinus carpio L. is as the temperate zone fresh-water fishes, very easily caused disease by intrusion or other environmental factors of microorganisms such as virus, fungus, antibacterial and unicellular alga.Product of the present invention has the oxidation resistance that can improve superoxide dismutase in the Cyprinus carpio L. liver and reduces MDA content in the liver, and product of the present invention is beneficial to suitability for industrialized production.
Summary of the invention
1, a kind of preparation method of compound poria cocos preparation is characterized in that the preparation method of said preparation comprises following process:
Step (one): select high-quality Poria 6g-9g, Polyporus 6g-9g, Pericarpium Citri Reticulatae 6g-9g, Radix Angelicae Sinensis 6g-9g, Radix Polygoni Multiflori 6g-9g, Radix Panacis Quinquefolii 6g-9g, Radix Et Caulis Acanthopanacis Senticosi 6g-9g, Rhizoma Polygonati 4g-6g, Rhizoma Dioscoreae 4g-6g, Fructus Crataegi 4g-6g, Fructus Hordei Germinatus 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound poria cocos preparation.
2, the purposes of a kind of compound poria cocos preparation as claimed in claim 1 is characterised in that it has the oxidation resistance that can improve superoxide dismutase in the Cyprinus carpio L. liver and reduces MDA content in the liver.
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
The preparation method and its usage that relates to a kind of compound poria cocos preparation of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
Step (one): select high-quality Poria 6g, Polyporus 6g, Pericarpium Citri Reticulatae 6g, Radix Angelicae Sinensis 6g, Radix Polygoni Multiflori 6g, Radix Panacis Quinquefolii 6g, Radix Et Caulis Acanthopanacis Senticosi 6g, Rhizoma Polygonati 4g, Rhizoma Dioscoreae 4g, Fructus Crataegi 4g, Fructus Hordei Germinatus 2g, Fructus Jujubae 2g and clean, microwave drying, pulverizing is 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6 times of water gagings first and soak 4.0h, heated and boiled is extracted 2.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound poria cocos preparation.
Embodiment 2:
Step (one): select high-quality Poria 9g, Polyporus 9g, Pericarpium Citri Reticulatae 9g, Radix Angelicae Sinensis 9g, Radix Polygoni Multiflori 9g, Radix Panacis Quinquefolii 9g, Radix Et Caulis Acanthopanacis Senticosi 9g, Rhizoma Polygonati 6g, Rhizoma Dioscoreae 6g, Fructus Crataegi 6g, Fructus Hordei Germinatus 4g, Fructus Jujubae 4g and clean, microwave drying, pulverizing is 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound poria cocos preparation.
Pharmacological evaluation
MDA content experiment in superoxide dismutase oxidation resistance and the reduction liver in the compound poria cocos preparation raising Cyprinus carpio L. liver
1 materials and methods
1.1 the preparation of compound poria cocos preparation test feed
Experiment consists of with compound poria cocos preparation: Poria 9g, Polyporus 9g, Pericarpium Citri Reticulatae 9g, Radix Angelicae Sinensis 9g, Radix Polygoni Multiflori 9g, Radix Panacis Quinquefolii 9g, Radix Et Caulis Acanthopanacis Senticosi 9g, Rhizoma Polygonati 6g, Rhizoma Dioscoreae 6g, Fructus Crataegi 6g, Fructus Hordei Germinatus 4g, Fructus Jujubae 4g, the compound poria cocos preparation composition is established three groups of Concentraton gradient, promptly on each flavor dose basis, reduce or increase by 25% crude drug amount, be prepared into basic, normal, high dosage group.Select the high-quality Poria by three groups of Concentraton gradient of compound poria cocos preparation, Polyporus, Pericarpium Citri Reticulatae, Radix Angelicae Sinensis, Radix Polygoni Multiflori, Radix Panacis Quinquefolii, Radix Et Caulis Acanthopanacis Senticosi, Rhizoma Polygonati, Rhizoma Dioscoreae, Fructus Crataegi, Fructus Hordei Germinatus, Fructus Jujubae is also cleaned respectively, microwave drying, pulverizing is 50 order powder, every group of powder added water boil to be extracted 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, get filtrate, filtrate is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound poria cocos preparation, low with what configure, in, the compound poria cocos preparation of high concentration was by 3: 5 foundation bait (shrimp meal 30g, bean cake powder 30g, multidimensional vitamin 0.5g, mixed inorganic 0.5g, sodium alginate 0.5g, starch 12.5g, flour 12.5g) ratio adds, and makes low, in, high dose group compound poria cocos preparation test feed.Do blank with foundation bait.
1.2 experiment fish and feeding and management thereof
Experiment uses Cyprinus carpio L. Cyprinus carpio L. available from Aquatic product market, Weihai, (41.4 ± 5.7) g, and long (10.78 ± 0.48) cm of body, the male and female dual-purpose is supported in indoor glass aquarium before the experiment beginning temporarily, with commercial feed it is satiated with food and throws something and feeds.As source of recycled water (pH7.6), 24h continues aeration aerating, keeps dissolved oxygen in water amount 5.0mg/L with the tap water of abundant aeration dechlorination, and water temperature is controlled at (23 ± 1) ℃, raises and train to begin after 7 days to test.Experiment is divided into 4 groups with Cyprinus carpio L. at random by body weight, and three experimental grouies are thrown something and fed with the compound poria cocos preparation test feed, the matched group normal feedstuff of throwing something and feeding, every group 20 tail, establish three parallel.Experiment periods every other day feeding volume is 1.5%～2% of a fish body constitution amount, and regularly throw something and feed during 7:00 and during 19:00 every day, and be 15 days experimental period.
1.3 method
1.3.1 sample collecting
Behind preceding 1 day jejunitas 24h of off-test, take out 6 tail fishes in each aquarium, dissect and get liver and weigh.
1.3.2 the mensuration of liver immune indexes
After getting liver organization, with the clean back of 4 ℃ normal saline flushings absorbent paper suck dry moisture, weigh, get the 0.5g tissue, the normal saline homogenate that adds 9 times of volumes, the centrifugal 15min of 4000r/min gets supernatant and measures superoxide dismutase SOD (lot number 20101110), malonaldehyde MDA (lot number 20101110), test kit builds up bio-engineering research by Nanjing and is provided, and assay method provides method to measure by test kit.
2 results
Compound poria cocos preparation is to the result that influences of superoxide dismutase SOD activity and malonaldehyde MDA in the Cyprinus carpio L. liver
See Table 1.
Table 1 compound poria cocos preparation is to the result that influences of superoxide dismutase activity and malonaldehyde in the Cyprinus carpio liver
Annotate: compare * P＜0.05 with matched group
By table 1 as seen, the middle and high dosage group of compound poria cocos preparation all can improve the oxidation resistance of superoxide dismutase SOD in the Cyprinus carpio L. liver and reduce the content of malonaldehyde MDA.
Claims (2)
1. the preparation method of a compound poria cocos preparation is characterized in that the preparation method of said preparation comprises following process:
Step (one): select high-quality Poria 6g-9g, Polyporus 6g-9g, Pericarpium Citri Reticulatae 6g-9g, Radix Angelicae Sinensis 6g-9g, Radix Polygoni Multiflori 6g-9g, Radix Panacis Quinquefolii 6g-9g, Radix Et Caulis Acanthopanacis Senticosi 6g-9g, Rhizoma Polygonati 4g-6g, Rhizoma Dioscoreae 4g-6g, Fructus Crataegi 4g-6g, Fructus Hordei Germinatus 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound poria cocos preparation.
2. the purposes of a kind of compound poria cocos preparation as claimed in claim 1 is characterized in that said preparation is used for preparing oxidation resistance that improves Cyprinus carpio L. liver superoxide dismutase and the preparation that reduces mda content effect in the liver.
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| CN104171376A (en) * | 2014-07-10 | 2014-12-03 | 南宁荣港生物科技有限公司 | Preparation method of traditional Chinese medicine contained fish feed additive |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1104039A (en) * | 1993-12-22 | 1995-06-28 | 黄明和 | Chengji additive for fodder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1104039A (en) * | 1993-12-22 | 1995-06-28 | 黄明和 | Chengji additive for fodder |
Non-Patent Citations (1)
| Title |
|---|
| 周宝宽.抗疲劳防治亚健康中药整理与研究.《中华中医药学刊》.2008,(第01期), * |
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