CN102085348B - Preparation method and application of compound radix bupleuri preparation - Google Patents
Preparation method and application of compound radix bupleuri preparation Download PDFInfo
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- CN102085348B CN102085348B CN2011100077334A CN201110007733A CN102085348B CN 102085348 B CN102085348 B CN 102085348B CN 2011100077334 A CN2011100077334 A CN 2011100077334A CN 201110007733 A CN201110007733 A CN 201110007733A CN 102085348 B CN102085348 B CN 102085348B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention belongs to the technical field of research and development of medical products. The invention relates to a preparation method and application of a compound radix bupleuri preparation. The preparation method comprises the following steps: selecting 4-6g of high-quality radix bupleuri, 4-6g of medlar, 4-6g of spirulina, 4-6g of mushroom, 4-6g of kelp, 4-6g of cordate houttuyniae, 4-6g of ginseng, 2-3g of star anise, 2-3g of ginger, 2-3g of garlic, 2-3g of hawthorn, 2-3g of Chinese date and 9-12g of shrimp meal, washing, carrying out microwave drying, and grinding into 50-mesh powder; adding water to the powder, boiling to extract three times, merging the extracting solutions, and filtering to obtain the filtrate; and concentrating the filtrate into extract, uniformly mixing the extract and starch at a ratio of 1:4, granulating with a 10-mesh sieve, drying, and then sieving with a 14-mesh sieve, thus obtaining the compound radix bupleuri preparation. The product has the function of promoting liver functions and protein metabolism of golden carps and is beneficial to industrial production.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of preparation method and its usage of compound bupleuri preparation.Product of the present invention has the Cyprinus carpio L. liver function of promotion and protein metabolism effect, and product of the present invention is beneficial to suitability for industrialized production.
Background technology
Advantage such as that Chinese herbal feed additive has is natural, economic, efficient, noresidue, no Drug resistance and side effect are little; And have the dual function of medical and trophism concurrently; The drawback of no antibiotics and chemicals; Be widely used in recent years in the aquaculture of aquatic animal, in order to prevent disease, growth promotion with improve aquatic product quality.Contain the various active composition in the Chinese herbal medicine, the scalable immunity function of animal.Therefore it is generally acknowledged Chinese herbal medicine prevent disease and growth promoting function, main can to improve the animal body immunologic function relevant with it.The feed additive of Fish has shortcomings such as the thawing of being prone to, poor stability at present, and a part can not be eaten by fish, and utilization rate is low, and waste is serious.Glutamate pyruvate transaminase (GPT), glutamic oxaloacetic transaminase, GOT (GOT) are aminotransferases important in the body protein metabolic process, and the active height of the two has reflected the situation of protein metabolism and liver function in the liver.The transaminase mainly is distributed in the cell under the normal condition, when liver dysfunction, has a large amount of transaminases and in hepatocyte, is released into blood, makes transaminase activity rising in the serum, and transaminase activity reduces in the hepatocyte simultaneously.The compound bupleuri preparation can make that transaminase activity generally raises in the Cyprinus carpio L. liver, explains that the compound bupleuri preparation does not have hepatotoxicity and the liver function and the protein metabolism of Cyprinus carpio L. had facilitation.
Summary of the invention
1, a kind of method for preparing of compound bupleuri preparation is characterized in that method of making preparation comprises following process:
Step (one): select high-quality Radix Bupleuri 4g-6g, Fructus Lycii 4g-6g, spirulina 4g-6g, Lentinus Edodes 4g-6g, Thallus Laminariae (Thallus Eckloniae) 4g-6g, Herba Houttuyniae 4g-6g, Radix Ginseng 4g-6g, Fructus Anisi Stellati 2g-3g, Rhizoma Zingiberis Recens 2g-3g, Bulbus Allii 2g-3g, Fructus Crataegi 2g-3g, Fructus Jujubae 2g-3g, shrimp meal 9g-12g and clean; Microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times; Add 6-8 times of water gaging first and soak 4.0h-6.0h; Heated and boiled is extracted 2.0h-3.0h; Add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h; Add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h; Merge extract, filter, must filtrate;
Step (three): the filtrating in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound bupleuri preparation.
2, the purposes of a kind of compound bupleuri preparation as claimed in claim 1 is characterised in that it has the Cyprinus carpio L. fish liver function of promotion and protein metabolism effect.
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
The preparation method and its usage that relates to a kind of compound bupleuri preparation of the present invention comprises following examples, and following embodiment can further specify the present invention, but does not limit the present invention in any way.
Embodiment 1:
Step (one): select high-quality Radix Bupleuri 4g, Fructus Lycii 4g, spirulina 4g, Lentinus Edodes 4g, Thallus Laminariae (Thallus Eckloniae) 4g, Herba Houttuyniae 4g, Radix Ginseng 4g, Fructus Anisi Stellati 2g, Rhizoma Zingiberis Recens 2g, Bulbus Allii 2g, Fructus Crataegi 2g, Fructus Jujubae 2g, shrimp meal 9g and clean; Microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6 times of water gagings first and soak 4.0h, heated and boiled is extracted 2.0h; Add for the second time 4 times of water gaging heated and boiled and extract 1.5h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h, merge extractive liquid; Filter, must filtrate;
Step (three): the filtrating in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound bupleuri preparation.
Embodiment 2:
Step (one): select high-quality Radix Bupleuri 6g, Fructus Lycii 6g, spirulina 6g, Lentinus Edodes 6g, Thallus Laminariae (Thallus Eckloniae) 6g, Herba Houttuyniae 6g, Radix Ginseng 6g, Fructus Anisi Stellati 3g, Rhizoma Zingiberis Recens 3g, Bulbus Allii 3g, Fructus Crataegi 3g, Fructus Jujubae 3g, shrimp meal 12g and clean; Microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h; Add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid; Filter, must filtrate;
Step (three): the filtrating in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound bupleuri preparation.
Pharmacological evaluation
The compound bupleuri preparation promotes Cyprinus carpio L. liver function and protein metabolism effect experiment
1 materials and methods
1.1 material
1.1.1 compound bupleuri preparation
The compound bupleuri preparation consists of: Radix Bupleuri 6g, Fructus Lycii 6g, spirulina 6g, Lentinus Edodes 6g, Thallus Laminariae (Thallus Eckloniae) 6g, Herba Houttuyniae 6g, Radix Ginseng 6g, Fructus Anisi Stellati 3g, Rhizoma Zingiberis Recens 3g, Bulbus Allii 3g, Fructus Crataegi 3g, Fructus Jujubae 3g, shrimp meal 12g; Compound bupleuri preparation composition is established three groups of Concentraton gradient; Promptly on each flavor dose basis, reduce or increase by 25% crude drug amount, be prepared into basic, normal, high dose groups.Form three groups of Concentraton gradient by the compound bupleuri preparation and select high-quality Radix Bupleuri, Fructus Lycii, spirulina, Lentinus Edodes, Thallus Laminariae (Thallus Eckloniae), Herba Houttuyniae, Radix Ginseng, Fructus Anisi Stellati, Rhizoma Zingiberis Recens, Bulbus Allii, Fructus Crataegi, Fructus Jujubae, shrimp meal and clean respectively, microwave drying, pulverizing is 50 order powder; Every group of powder added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h; Add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid; Filter, must filtrate, filtrating is condensed into extractum; In extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound bupleuri preparation.
1.1.2 experiment feedstuff
By the compound bupleuri preparation: the mixed uniform preparation of normal feedstuff=3: 5 is done blank for compound bupleuri preparation experiment feedstuff with normal feedstuff.The normal feedstuff prescription is used in experiment, sees table 1.
Table 1 experiment is filled a prescription with normal feedstuff
1.2 method
1.2.1 experiment is raised with Cyprinus carpio L.
Experiment with Cyprinus carpio L. Cyprinus carpio L. available from Aquatic product market, Weihai, body weight (41.1 ± 5.0) g, long (10.27 ± 0.48) cm of body, the male and female dual-purpose, experiment is supported in indoor glass aquarium before beginning temporarily, with commercial feed it is satiated with food and throws something and feeds.As source of recycled water, 24h continues aeration aerating, keeps dissolved oxygen in water amount 5.0mg/L with the tap water of abundant aeration dechlorination, and water temperature is controlled at (21 ± 1) ℃, raises and train to begin after 7 days to test.Experiment is divided into 2 groups with Cyprinus carpio L. by body weight at random, and experimental group is with compound bupleuri preparation experiment feedstuff feeding, the matched group normal feedstuff of throwing something and feeding, every group 20 tail, establish 3 parallel.Experimental period every other day feeding volume is 2%~3% of a fish body constitution amount, and regularly throw something and feed during 7:00 and during 19:00 every day, and be 15 days experimental period.
1.2.2 measure the collection of sample
The experiment end began jejunitas 24h in preceding 1 day, got 5 tail fishes at random from each aquarium, dissected and got liver, and is for use.
1.2.3 the mensuration of liver biochemical indexes
Get liver organization, use the absorbent paper suck dry moisture, weigh with the clean back of 4 ℃ normal saline flushings; Get the 0.5g tissue; Add the normal saline homogenate of 9 times of volumes, the centrifugal 15min of 3000r/min gets liver tissue homogenate's supernatant of 10%; The dilution of use normal saline is 1% homogenate, measures related biochemical indicator.
(1) gpt activity (GPT): the reitman-frankel method that adopts GPT test kit (bio-engineering research institute, lot number 20101112 are built up in Nanjing) to provide is measured.
(2) glutamic oxaloacetic transaminase, GOT (GOT): the reitman-frankel method that adopts GOT test kit (bio-engineering research institute, lot number 20101111 are built up in Nanjing) to provide is measured.
2 results
The compound bupleuri preparation is to glutamate pyruvate transaminase (GPT), the active result that influences of glutamic oxaloacetic transaminase, GOT (GOT) in the Cyprinus carpio L. liver
See table 2.
Table 2 compound bupleuri preparation is to the result that influences of glutamate pyruvate transaminase, glutamic oxaloacetic transaminase, GOT in the Cyprinus carpio L. liver
Annotate: compare with matched group,
*P<0.05
Visible by table 2, with matched group relatively, can the raise activity of glutamate pyruvate transaminase in the Cyprinus carpio L. liver, glutamic oxaloacetic transaminase, GOT of compound bupleuri preparation explains that the compound bupleuri preparation does not have hepatotoxicity and the liver function and the protein metabolism of Cyprinus carpio L. had facilitation.In the compound bupleuri preparation in the dose groups rising Cyprinus carpio L. liver glutamate pyruvate transaminase effect remarkable,
Claims (2)
1. the method for preparing of a compound bupleuri fancy carp feed is characterized in that the method for preparing of this feedstuff comprises following process:
Step (one): select Radix Bupleuri 4g-6g, Fructus Lycii 4g-6g, spirulina 4g-6g, Lentinus Edodes 4g-6g, Thallus Laminariae (Thallus Eckloniae) 4g-6g, Herba Houttuyniae 4g-6g, Radix Ginseng 4g-6g, Fructus Anisi Stellati 2g-3g, Rhizoma Zingiberis Recens 2g-3g, Bulbus Allii 2g-3g, Fructus Crataegi 2g-3g, Fructus Jujubae 2g-3g, shrimp meal 9g-12g and clean; Microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times; Add 6-8 times of water gaging first and soak 4.0h-6.0h; Heated and boiled is extracted 2.0h-3.0h; Add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h; Add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h; Merge extract, filter, must filtrate;
Step (three): the filtrating in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound bupleuri fancy carp feed.
2. the purposes of a kind of compound bupleuri fancy carp feed as claimed in claim 1 is characterized in that this feedstuff is used to prepare the preparation with promotion Cyprinus carpio L. fish liver function and protein metabolism effect.
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101371915A (en) * | 2007-08-24 | 2009-02-25 | 曾雄辉 | Method for preparing minor decoction of Bupleurum formulation |
CN101491300A (en) * | 2009-03-05 | 2009-07-29 | 浙江大学 | Feed additive capable of preventing and treating large yellow croaker fatty liver |
CN101889664A (en) * | 2010-04-22 | 2010-11-24 | 广东海洋大学 | Chinese herb medicinal additive for preventing tilapia fatty liver disease |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN101371915A (en) * | 2007-08-24 | 2009-02-25 | 曾雄辉 | Method for preparing minor decoction of Bupleurum formulation |
CN101491300A (en) * | 2009-03-05 | 2009-07-29 | 浙江大学 | Feed additive capable of preventing and treating large yellow croaker fatty liver |
CN101889664A (en) * | 2010-04-22 | 2010-11-24 | 广东海洋大学 | Chinese herb medicinal additive for preventing tilapia fatty liver disease |
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