CN102085290B - Preparation method and application of compound rhodiola rosea preparation - Google Patents

Abstract

The invention belongs to the technical field of research and development of medical products. The invention relates to a preparation method and application of a compound rhodiola rosea preparation. The preparation method comprises the following steps: selecting 6-12g of high-quality rhodiola rosea, 6-9g of eucommia bark, 3-6g of acanthopanax, 3-6g of ginseng, 3-6g of lucid ganoderma, 3-6g of radix astragali, 3-6g of mushroom, 2-4g of poria, 2-4g of grifola, 2-4g of Chinese yam, 2-4g of hawthorn and 2-4g of Chinese date, washing, carrying out microwave drying, and grinding into 50-mesh powder; adding water to the powder, boiling to extract three times, merging the extracting solutions, and filtering to obtain the filtrate; and concentrating the filtrate into extract, uniformly mixing the extract, agar and starch at a ratio of 4:1:16, granulating with a 10-mesh sieve, drying, and then sieving with a 14-mesh sieve, thus obtaining the compound rhodiola rosea preparation. The product has the function of promoting growth of golden carps and is beneficial to industrial production.

Description

A kind of preparation method and its usage of compound rhodiola root preparation

Technical field

Medical product research and development technical field under the present invention.The present invention relates to a kind of preparation method and its usage of compound rhodiola root preparation.Product of the present invention has facilitation to fancy carp growth, and product of the present invention is beneficial to suitability for industrialized production.

Background technology

Advantage such as that Chinese herbal feed additive has is natural, economic, efficient, noresidue, no Drug resistance and side effect are little, and have the dual function of medical and trophism concurrently, the drawback of no antibiotics and chemicals, be widely used in recent years in the aquaculture of aquatic animal, in order to prevent disease, growth promotion with improve aquatic product quality.Contain the various active composition in the Chinese herbal medicine, the scalable immunity function of animal.Therefore mainly can to improve the animal body immunologic function relevant with it to it is generally acknowledged Chinese herbal medicine prevent disease and growth promoting function.The feed additive of Fish has shortcomings such as easy thawing, poor stability at present, and a part can not be eaten by fish, and utilization rate is low, and waste is serious.Agar (Agar) claims agar again, is the hydrophilic macromolecule polysaccharide that extracts from the Bostrychia montagnei guiding principle, has good gelling and stability, be widely used in aspects such as food, medicine, biological engineering, but the application in the fish feed additive is not reported.

Summary of the invention

1, a kind of preparation method of compound rhodiola root preparation is characterized in that the preparation method of said preparation comprises following process:

Step (one): select high-quality Radix Rhodiolae 6g-12g, Cortex Eucommiae 6g-9g, Radix Et Caulis Acanthopanacis Senticosi 3g-6g, Radix Ginseng 3g-6g, Ganoderma 3g-6g, Radix Astragali 3g-6g, Lentinus Edodes 3g-6g, Poria 2g-4g, Polyporus 2g-4g, Rhizoma Dioscoreae 2g-4g, Fructus Crataegi 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound rhodiola root preparation.

2, a kind of purposes of compound rhodiola root preparation is characterised in that it has facilitation to fancy carp growth.

According to the present invention, " % " among the present invention is percentage by weight.

The specific embodiment

The preparation method and its usage that relates to a kind of compound rhodiola root preparation of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.

Embodiment 1:

Step (one): select high-quality Radix Rhodiolae 6g, Cortex Eucommiae 6g, Radix Et Caulis Acanthopanacis Senticosi 3g, Radix Ginseng 3g, Ganoderma 3g, Radix Astragali 3g, Lentinus Edodes 3g, Poria 2g, Polyporus 2g, Rhizoma Dioscoreae 2g, Fructus Crataegi 2g, Fructus Jujubae 2g and clean, microwave drying, pulverizing is 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 6 times of water gagings first and soak 4.0h, heated and boiled is extracted 2.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h, merge extractive liquid,, filter, get filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound rhodiola root preparation.

Embodiment 2:

Step (one): select high-quality Radix Rhodiolae 12g, Cortex Eucommiae 9g, Radix Et Caulis Acanthopanacis Senticosi 6g, Radix Ginseng 6g, Ganoderma 6g, Radix Astragali 6g, Lentinus Edodes 6g, Poria 4g, Polyporus 4g, Rhizoma Dioscoreae 4g, Fructus Crataegi 4g, Fructus Jujubae 4g and clean, microwave drying, pulverizing is 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, get filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound rhodiola root preparation.

Pharmacological evaluation

The compound rhodiola root preparation is to the facilitation experiment of fancy carp growth

1 materials and methods

1.1 material

1.1.1 compound rhodiola root preparation

Select high-quality Radix Rhodiolae 12g, Cortex Eucommiae 9g, Radix Et Caulis Acanthopanacis Senticosi 6g, Radix Ginseng 6g, Ganoderma 6g, Radix Astragali 6g, Lentinus Edodes 6g, Poria 4g, Polyporus 4g, Rhizoma Dioscoreae 4g, Fructus Crataegi 4g, Fructus Jujubae 4g also cleans, microwave drying, pulverizing is 50 order powder, powder is added water boil to be extracted 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, filtrate, filtrate is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound rhodiola root preparation.

1.1.2 experiment feedstuff

By the compound rhodiola root preparation: the mixed uniform preparation of normal feedstuff=3: 5 is done blank for compound rhodiola root preparation experiment feedstuff with normal feedstuff.Experiment normal feedstuff prescription sees Table 1.

Table 1 experiment is filled a prescription with normal feedstuff

1.2 method

1.2.1 experiment is raised with Cyprinus carpio L.

Experiment uses Cyprinus carpio L. Cyprinus carpio L. available from Aquatic product market, Weihai, body weight (41.1 ± 5.0) g, and long (10.27 ± 0.48) cm of body, the male and female dual-purpose is supported in indoor glass aquarium before the experiment beginning temporarily, with commercial feed it is satiated with food and throws something and feeds.As source of recycled water, 24h continues aeration aerating, keeps dissolved oxygen in water amount 5.0mg/L with the tap water of abundant aeration dechlorination, and water temperature is controlled at (21 ± 1) ℃, raises and train to begin after 7 days to test.Experiment is divided into 2 groups with Cyprinus carpio L. at random by body weight, and experimental group is with compound rhodiola root preparation experiment feedstuff feeding, the matched group normal feedstuff of throwing something and feeding, every group 20 tail, establish 3 parallel.Experiment periods every other day feeding volume is 2%～3% of a fish body constitution amount, and regularly throw something and feed during 7:00 and during 19:00 every day, and be 15 days experimental period.

1.2.2 the compound rhodiola root preparation is measured the facilitation and the feed coefficient of Cyprinus carpio L. body weight

The experiment initial stage is measured length of fish body and fish body weight, and record, when experiment finishes, length of fish body and fish body weight is measured again.Collect Excreta and the bait residue of experiment fish simultaneously, weigh after the oven dry.The matched group fish is raised the same experimental group of condition, throws something and feeds foundation bait in contrast.

Rate of body weight gain=[(testing last fish body counterpoise-experiment fish body counterpoise just)/experiment is fish body counterpoise just] * 100%

Feed coefficient=(bait consumption/fish body weight gains amount) * 100%.

1.2.3 the compound rhodiola root preparation is to the mensuration of Cyprinus carpio L. richness influence

Richness (%)=(test last fish body weight/test last length of fish body) * 100%

1.2.4 the compound rhodiola root preparation is to the mensuration of Cyprinus carpio L. liver body than influence

Experimental group and matched group are all raised by daily mode.Randomly draw 6 of experiment fishes from each group during the experiment beginning, put to death the back of weighing, and dissects along cloacal aperture, and the clip liver is also weighed.When finishing, experiment takes identical operations.Liver body ratio=(testing the last fish body weight of last fish body liver weight/experiment) * 100%

2 results

What the compound rhodiola root preparation promoted fancy carp growth the results are shown in Table 2.

Table 2 compound rhodiola root preparation promotes the result of fancy carp growth

Annotate: compare * P＜0.05 with matched group

By table 2 as seen, the compound rhodiola root preparation can significantly improve rate of body weight gain, coefficient of condition, the liver body ratio of Cyprinus carpio L., and can reduce feed coefficient.

Claims (2)

1. the preparation method of a compound rhodiola root preparation is characterized in that the preparation method of said preparation comprises following process:

Step (one): select high-quality Radix Rhodiolae 6g-12g, Cortex Eucommiae 6g-9g, Radix Et Caulis Acanthopanacis Senticosi 3g-6g, Radix Ginseng 3g-6g, Ganoderma 3g-6g, Radix Astragali 3g-6g, Lentinus Edodes 3g-6g, Poria 2g-4g, Polyporus 2g-4g, Rhizoma Dioscoreae 2g-4g, Fructus Crataegi 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got the compound rhodiola root preparation.

2. the purposes of the compound rhodiola root preparation of the preparation method preparation of compound rhodiola root preparation as claimed in claim 1 is characterised in that it has facilitation to fancy carp growth.