CN102078484B - Preparation method and application of compound capillary artemisia preparation - Google Patents
Preparation method and application of compound capillary artemisia preparation Download PDFInfo
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- CN102078484B CN102078484B CN201110008822A CN201110008822A CN102078484B CN 102078484 B CN102078484 B CN 102078484B CN 201110008822 A CN201110008822 A CN 201110008822A CN 201110008822 A CN201110008822 A CN 201110008822A CN 102078484 B CN102078484 B CN 102078484B
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- artemisiae scopariae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the technical fields of research and development of medical products. The invention relates to a preparation method and application of a compound capillary artemisia preparation. The preparation method comprises the following steps: selecting the following high-quality raw materials: 6-9g of capillary artemisia, 6-9g of bupleurum, 6-9g of shiandra, 6-9g of rhodiola root, 3-6g of eucommia, 3-6g of acanthopanax, 3-6g of ginseng, 3-6g of ganoderma, 3-6g of milk vetch, 3-6g of mushroom, 2-4g of tuckahoe, 2-4g of grifola, 2-4g of Chinese yam, 2-4g of hawthorn and 2-4g of jujube; cleaning the raw materials; drying the raw materials by microwaves; crushing the raw materials into 50-mesh powder; adding water for boiling, and extracting for 3 times; merging the extracting solutions; filtering to obtain a filtered solution; concentrating the filtered solution into an extract; evenly mixing the extract, agar and starch according to the ratio of 4:1:16; granulating with a 10-mesh sieve; baking; and then, sieving with a 14-mesh sieve to obtain the compound capillary artemisia preparation. The product which is prepared by the preparation method provided by the invention has the functions of improving total antioxidant capacity and lysozyme activity in the liver of a fancy carp, and is favorable for industrialized production.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of preparation method and its usage of compound recipe Herba Artemisiae Scopariae preparation.Product of the present invention has can improve Total antioxidant capacity and lysozyme activity in the Cyprinus carpio L. liver, and product of the present invention is beneficial to suitability for industrialized production.
Background technology
Advantage such as that Chinese herbal feed additive has is natural, economic, efficient, noresidue, no Drug resistance and side effect are little, and have the dual function of medical and trophism concurrently, the drawback of no antibiotics and chemicals, be widely used in recent years in the aquaculture of aquatic animal, in order to prevent disease, growth promotion with improve aquatic product quality.Contain the various active composition in the Chinese herbal medicine, the scalable immunity function of animal.Therefore mainly can to improve the animal body immunologic function relevant with it to it is generally acknowledged Chinese herbal medicine prevent disease and growth promoting function.The feed additive of Fish has shortcomings such as easy thawing, poor stability at present, and a part can not be eaten by fish, and utilization rate is low, and waste is serious.Agar (Agar) claims agar again, is the hydrophilic macromolecule polysaccharide that extracts from the Bostrychia montagnei guiding principle, has good gelling and stability, be widely used in aspects such as food, medicine, biological engineering, but the application in the fish feed additive is not reported.Total antioxidant capacity (T-AOC) is meant by enzymatic systems such as glutathion peroxidase, catalases with by V
E, glutathion, V
CTotal oxidation resistance etc. non-enzymatic system composition, lysozyme (LZM) is the alkaline enzyme of mucopolysaccharide in a kind of energy hydrolysis pathogenic bacterium, having effects such as antibiotic, antiinflammatory, antiviral, is a kind of important nonspecific defense factor, and its activity is directly connected to the immune function of Fish.Contain effective ingredient such as alkaloid, glycoside, saccharide, organic acid in the Chinese herbal medicine, has immunomodulating and potentiation, select the multiple not Chinese herbal medicine composition compound Chinese medicinal preparation of same-action that has, can obtain drug effect complementation, the enhanced advantage of curative effect, on reality is used, have more advantage.A kind of compound recipe Herba Artemisiae Scopariae preparation can improve Total antioxidant capacity and lysozyme activity in the Cyprinus carpio liver in the test.
Summary of the invention
1, a kind of preparation method of compound recipe Herba Artemisiae Scopariae preparation is characterized in that the preparation method of said preparation comprises following process:
Step (one): select high-quality Herba Artemisiae Scopariae 6g-9g, Radix Bupleuri 6g-9g, Fructus Schisandrae Chinensis 6g-9g, Radix Rhodiolae 6g-9g, Cortex Eucommiae 3g-6g, Radix Et Caulis Acanthopanacis Senticosi 3g-6g, Radix Ginseng 3g-6g, Ganoderma 3g-6g, Radix Astragali 3g-6g, Lentinus Edodes 3g-6g, Poria 2g-4g, Polyporus 2g-4g, Rhizoma Dioscoreae 2g-4g, Fructus Crataegi 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Herba Artemisiae Scopariae preparation.
2, the purposes of a kind of compound recipe Herba Artemisiae Scopariae preparation as claimed in claim 1, being characterised in that it has can improve Total antioxidant capacity and lysozyme activity in the Cyprinus carpio L. liver.
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
The preparation method and its usage that relates to a kind of compound recipe Herba Artemisiae Scopariae preparation of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
Step (one): select high-quality Herba Artemisiae Scopariae 6g, Radix Bupleuri 6g, Fructus Schisandrae Chinensis 6g, Radix Rhodiolae 6g, Cortex Eucommiae 3g, Radix Et Caulis Acanthopanacis Senticosi 3g, Radix Ginseng 3g, Ganoderma 3g, Radix Astragali 3g, Lentinus Edodes 3g, Poria 2g, Polyporus 2g, Rhizoma Dioscoreae 2g, Fructus Crataegi 2g, Fructus Jujubae 2g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h, heated and boiled is extracted 2.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Herba Artemisiae Scopariae preparation.
Embodiment 2:
Step (one): select high-quality Herba Artemisiae Scopariae 9g, Radix Bupleuri 9g, Fructus Schisandrae Chinensis 9g, Radix Rhodiolae 9g, Cortex Eucommiae 6g, Radix Et Caulis Acanthopanacis Senticosi 6g, Radix Ginseng 6g, Ganoderma 6g, Radix Astragali 6g, Lentinus Edodes 6g, Poria 4g, Polyporus 4g, Rhizoma Dioscoreae 4g, Fructus Crataegi 4g, Fructus Jujubae 4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Herba Artemisiae Scopariae preparation.
Pharmacological evaluation
Compound recipe Herba Artemisiae Scopariae preparation improves Total antioxidant capacity and lysozyme activity effect experiment in the Cyprinus carpio L. liver
1 materials and methods
1.1 material
1.1.1 compound recipe Herba Artemisiae Scopariae preparation
Select high-quality Herba Artemisiae Scopariae 9g, Radix Bupleuri 9g, Fructus Schisandrae Chinensis 9g, Radix Rhodiolae 9g, Cortex Eucommiae 6g, Radix Et Caulis Acanthopanacis Senticosi 6g, Radix Ginseng 6g, Ganoderma 6g, Radix Astragali 6g, Lentinus Edodes 6g, Poria 4g, Polyporus 4g, Rhizoma Dioscoreae 4g, Fructus Crataegi 4g, Fructus Jujubae 4g also cleans, microwave drying, pulverizing is 50 order powder, powder is added water boil to be extracted 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, filtrate, filtrate is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Herba Artemisiae Scopariae preparation.
1.1.2 experiment feedstuff
By compound recipe Herba Artemisiae Scopariae preparation: the mixed uniform preparation of normal feedstuff=3: 5 is a compound recipe Herba Artemisiae Scopariae preparation experiment feedstuff, does blank with normal feedstuff.Experiment normal feedstuff prescription sees Table 1.
Table 1 experiment is filled a prescription with normal feedstuff
1.2 method
1.2.1 experiment is raised with Cyprinus carpio L.
Experiment uses Cyprinus carpio L. Cyprinus carpio L. available from Aquatic product market, Weihai, body weight (41.1 ± 5.0) g, and long (10.27 ± 0.48) cm of body, the male and female dual-purpose is supported in indoor glass aquarium before the experiment beginning temporarily, with commercial feed it is satiated with food and throws something and feeds.As source of recycled water, 24h continues aeration aerating, keeps dissolved oxygen in water amount 5.0mg/L with the tap water of abundant aeration dechlorination, and water temperature is controlled at (21 ± 1) ℃, raises and train to begin after 7 days to test.Experiment is divided into 2 groups with Cyprinus carpio L. at random by body weight, and experimental group is with compound recipe Herba Artemisiae Scopariae preparation experiment feedstuff feeding, the matched group normal feedstuff of throwing something and feeding, every group 20 tail, establish 3 parallel.Experiment periods every other day feeding volume is 2%～3% of a fish body constitution amount, and regularly throw something and feed during 7:00 and during 19:00 every day, and be 15 days experimental period.
1.2.2 measure the collection of sample
The experiment end began jejunitas 24h in preceding 1 day, got 5 tail fishes at random from each aquarium, dissected and got liver, and is stand-by.
1.2.3 the mensuration of liver biochemical indexes
Get liver organization, use the absorbent paper suck dry moisture with the clean back of 4 ℃ normal saline flushings, weigh, get the 0.5g tissue, add the normal saline homogenate of 9 times of volumes, the centrifugal 15min of 3000r/min gets supernatant and measures the related immune index.
(1) Total antioxidant capacity (T-AOC) is measured: the method mensuration that adopts T-AOC test kit (bio-engineering research institute, lot number 20101102 are built up in Nanjing) and provide.
(2) lysozyme activity (LZM) is measured: self turbidimetry for Determination that adopts LZM test kit (bio-engineering research institute, lot number 20101027 are built up in Nanjing).
2 results
Compound recipe Herba Artemisiae Scopariae preparation improves Total antioxidant capacity and lysozyme activity exercising result in the Cyprinus carpio L. liver
See Table 2.
Table 2 compound recipe Herba Artemisiae Scopariae preparation improves Total antioxidant capacity and lysozyme activity exercising result in the Cyprinus carpio L. liver
Annotate: compare * P＜0.05 with matched group
By table 2 as seen, can raise Total antioxidant capacity and activity of lysozyme in the Cyprinus carpio L. liver of compound recipe Herba Artemisiae Scopariae preparation.
Claims (2)
1. the preparation method of a compound recipe Herba Artemisiae Scopariae preparation is characterized in that the preparation method of said preparation comprises following process:
Step (one): select high-quality Herba Artemisiae Scopariae 6g-9g, Radix Bupleuri 6g-9g, Fructus Schisandrae Chinensis 6g-9g, Radix Rhodiolae 6g-9g, Cortex Eucommiae 3g-6g, Radix Et Caulis Acanthopanacis Senticosi 3g-6g, Radix Ginseng 3g-6g, Ganoderma 3g-6g, Radix Astragali 3g-6g, Lentinus Edodes 3g-6g, Poria 2g-4g, Polyporus 2g-4g, Rhizoma Dioscoreae 2g-4g, Fructus Crataegi 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Herba Artemisiae Scopariae preparation.
2. the purposes of a kind of compound recipe Herba Artemisiae Scopariae preparation as claimed in claim 1 is characterized in that said preparation is used for preparing the preparation that improves Cyprinus carpio L. liver Total antioxidant capacity and lysozyme activity effect.
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| CN105146427A (en) * | 2015-08-07 | 2015-12-16 | 哈尔滨天一生态农副产品有限公司 | Dried virgate wormwood herb capable of clearing heat and removing toxicity and preparation method of virgate wormwood herb |
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| CN101791054A (en) * | 2010-04-28 | 2010-08-04 | 中国水产科学研究院黑龙江水产研究所 | Game fish Chinese herbal medicine complex additive |
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