CN102763782B - Compound Chinese herbal medicine preparation for improving tilapia immunity - Google Patents
Compound Chinese herbal medicine preparation for improving tilapia immunity Download PDFInfo
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- CN102763782B CN102763782B CN 201210270818 CN201210270818A CN102763782B CN 102763782 B CN102763782 B CN 102763782B CN 201210270818 CN201210270818 CN 201210270818 CN 201210270818 A CN201210270818 A CN 201210270818A CN 102763782 B CN102763782 B CN 102763782B
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Abstract
A compound Chinese herbal medicine preparation for improving tilapia immunity is obtained by mixing the following components, by weight, 40% of houttuynia cordata, 5% of radix scutellariae, 5% of isatis root, 5% of fructus forsythiae, 10% of honeysuckle, 5% of liquorice, 5% of rheum officinale, 10% of astragalus, 5% of angelica sinensis and 10% of hawthorn. The compound Chinese herbal medicine preparation can remarkably improve activity of superoxide dismutase (SOD) in tilapia serum, the content of nitric oxide (NO), the activity of serum lysoyme (LSZ), phagocytic percentage, phagocytic index and respiratory burst, greatly reduces the content of serum malondialdehyde (MDA) of tilapia, and effectively improves the tilapia immunity. Feed containing the compound Chinese herbal medicine preparation is further disclosed. The production process is simple, the compound Chinese herbal medicine preparation can be added into commodity feed directly and can not affect taste of the feed, Chinese herbal medicine raw materials used in the preparation are low in price, wide and stable in sources, and the preparation can be produced in industrialization mode.
Description
Technical field
The present invention relates to Chinese herbal feed additive, specifically can improve the compound Chinese herbal medicine additive of Tilapia mossambica immunity.
Background technology
Tilapia mossambica is that one of fish of culturing are recommended by FAO (Food and Agriculture Organization of the United Nation) to countries in the world, it is assorted to have feeding habits, growth is fast, the population yield height, characteristics such as adaptability is strong, extensively culture in south China tropical and subtropical zone area in recent years, become the breed variety that China has competitiveness in the international market most at present.Yet, being accompanied by large-area breed, various disease problems also become increasingly conspicuous, and especially streptococcus is caused serious threat to China's Tilapia mossambica industry especially.Usually use antibiotic medicine to be used for treating streptococcosis in the production, but a large amount of antibiotic abuses can bring medicine residual again, cause more serious food-safety problem.Therefore, the disease problem has become restriction Tilapia mossambica industry and has further developed, influenced one of principal element of product quality.Discover that adding some Chinese herbal medicines in feed can improve the autoimmune power of fish body, builds up one's resistance to disease.
Chinese herbal medicine is remote in the use history of China, mainly is because advantages such as it is efficient, low toxicity, low-residual, and contains panimmunity and regulate active component such as alkaloid, polysaccharide, saponin, anthracene class, volatile oil, organic acid etc.Some scholar's research the influence of Chinese herbal medicine to fish immunity function and diseases prevention anti-stress ability.Can effectively suppress salmon rhabdovirus and viral hemorrhagic leukemia virus as the oleuropein that from olive growing leaves, extracts.Radix-polygoni multiflori extract (PME) Wheat Protein helps the removing of interior free yl.In feed, add the Turmeric P.E rainbow trout of feeding and significantly to increase cytophagous phagocytosis in the blood.Nutrient substances such as these organic acids that contain owing to Chinese herbal medicine, tannin, alkaloid, flavones, glycoside, inorganic salts can impel the synthetic of organism metabolism and protein in addition, thereby accelerate the speed of growth and improve meat quality.Rate of body weight gain, specific growth rate, the protein content of Luo Fei juvenile fish be can effectively improve as in basal feed, adding more than 20 kind of Chinese herbal medicines such as the Radix Astragali, honeysuckle, kuh-seng, Chinese violet, feed coefficient and crude fat content reduced.Therefore, the research Chinese herbal medicine is to the effect of Tilapia mossambica immunity, and the Chinese herbal medicine immunopotentiator of developing efficient, safety, environmental protection is significant to the sound development of Tilapia mossambica industry.
Summary of the invention
The purpose of this invention is to provide a kind of Chinese herbal formula that improves Tilapia mossambica immunity, this Chinese herbal formula can effectively improve the immunity of Tilapia mossambica, reduces the generation of Tilapia mossambica disease.
The present invention also aims to provide and contain the above-mentioned tilapia feed that can improve the Chinese herbal formula of Tilapia mossambica immunity, this feed production technology is simple and convenient, and this tilapia feed that contains Chinese herbal formula can effectively improve the immunity of Tilapia mossambica by throwing something and feeding.
For realizing first purpose of the present invention, a kind of Chinese herbal formula that improves Tilapia mossambica immunity provided by the invention obtains by following component and weight proportion mixing:
Cordate houttuynia (40%), the root of large-flowered skullcap (5%), Radix Isatidis (5%), the capsule of weeping forsythia (5%), honeysuckle (10%), Radix Glycyrrhizae (5%), rheum officinale (5%), the Radix Astragali (10%), Radix Angelicae Sinensis (5%), hawthorn (10%).
The preparation method of the Chinese herbal formula of above-mentioned raising Tilapia mossambica immunity is: earlier the ultra micro powder is made in each component oven dry, mixed by above-mentioned weight proportion.
For realizing second purpose of the present invention, the invention provides and contain the above-mentioned tilapia feed that can improve the Chinese herbal formula of Tilapia mossambica immunity, above-mentioned each component raw material drying is made the ultra micro powder, mix Chinese herbal formula by above-mentioned weight proportion again, Chinese herbal formula by weight percentage for 3-6% joins in the tilapia feed, is made a material feed of respective diameters size behind the mixing.
Advantage of the present invention is: Chinese herbal formula of the present invention can significantly improve superoxide dismutase in the Tilapia mossambica serum (SOD) vigor, nitric oxide (NO) content, serum lysozyme (LSZ) vigor, percentage phagocytosis, phagocytic index and respiratory burst vigor, significantly reduce Tilapia mossambica serum MDA (MDA) content, effectively improve the immunity of Tilapia mossambica, reduce the generation of Tilapia mossambica disease.Contain the feed of this Chinese herbal formula, production technology is simple, can directly add in the commercial feed, can the palatability of feed not exerted an influence, and used herbal raw material is cheap, and wide material sources are stable, are easy to industrialization production.
The specific embodiment
1, the Chinese herbal formula that provides of this example, mix to obtain by following component and weight proportion:
Cordate houttuynia is 40%, the root of large-flowered skullcap is 5%, Radix Isatidis is 5%, the capsule of weeping forsythia is 5%, honeysuckle is 10%, Radix Glycyrrhizae is 5%, rheum officinale is 5%, the Radix Astragali is 10%, when be classified as 5%, hawthorn is 10%.
Its preparation method is: earlier the ultra micro powder is made in each component oven dry, mixed Chinese herbal formula by above-mentioned weight proportion again.
2, the invention provides the tilapia feed that contains the Chinese herbal formula that can improve Tilapia mossambica immunity, be 40% by the method in the example 1 with cordate houttuynia, the root of large-flowered skullcap is 5%, Radix Isatidis is 5%, the capsule of weeping forsythia is 5%, honeysuckle is 10%, Radix Glycyrrhizae is 5%, rheum officinale is 5%, the Radix Astragali is 10%, when being classified as 5%, hawthorn is that each component raw material drying of 10% is made the ultra micro powder, mix Chinese herbal formula by above-mentioned weight proportion again, be 0 (control group) with Chinese herbal formula by mass fraction, 1% (A group), 3% (B group), 6% (C group) added in the basic tilapia feed, making diameter behind the mixing is the material feed of 3-5mm, use oven for drying, make 4 kinds of test feeds.
3, experimental design and feeding and management: the Tilapia mossambica that selects neat specification, physique stalwartness, average body quality and be 54 ± 2.32g is divided into 4 groups at random, put into 12 cement pits (470cm * 270cm * 80cm), every pond 70 tails, the above-mentioned 4 kinds of test feeds of throwing something and feeding respectively, establish 3 repetitions for every group, test was carried out for 12 weeks continuously.Test water is for through the running water of aeration, and with inflator pump oxygenation, a day feeding volume is fish body weight 3%, in the morning every day 9:00 and afternoon 5:00 respectively throw something and feed half.Begin formal test after raising and train 14d, the duration of test water temperature is (29 ± 2) ℃, and duration of test water body DO remains on more than the 5mg/L, pH value 7.5 ~ 8.0, and regularly add and change water.
4, above-mentioned the stating during the feed of throwing something and feeding respectively at each sampling of the 4th, 8,12 weeks once, measured Tilapia mossambica haematogenic immunity index.Get 12 tails (every case 4 tails) at random for each every group, take a blood sample in tail vein place with syringe.Only gather the mensuration that serum carries out the relevant enzyme vigor preceding twice.Anticoagulation and non-anticoagulation are collected in blood sampling at twice respectively for the last time.A part of the blood of collection is whole blood with aseptic liquaemin anti-freezing, is used for measuring the respiratory burst vigor of the cytophagous phagocytic activity of blood and haemocyte.Go to rapidly after the non-anticoagulation collection in the aseptic centrifuge tube of 2ml, leave standstill under the room temperature treat its blood coagulation after, 4 ℃ of 3000r/min are centrifugal, collect serum and be stored in-80 ℃ of ultra low temperature freezers, be used for measuring serum superoxide dismutase (SOD), lysozyme (LSZ), nitric oxide (NO), MDA enzyme activity and content such as (MDA).
5, result of the test shows: since the 8th week, B group bolti serum MDA (MDA) content reduces very significantly (P<0.01), and bolti serum lysozyme (LSZ) vigor significantly improves (P<0.05) in the C group, superoxide dismutase (SOD) vigor, nitric oxide (NO) content improve very significantly (P<0.01), and MDA content reduces (P<0.01) extremely significantly.To the 12nd week, the active utmost point of bolti serum activity of SOD and haemocyte respiratory burst significantly improves (P<0.01) in the B group, and serum LSZ vigor, NO content significantly improve (P<0.05); In the C group bolti serum activity of SOD, NO content, serum LSZ vigor, percentage phagocytosis, phagocytic index, respiratory burst vigor all the utmost point significantly improve (P<0.01), MDA content then reduces very significantly (P<0.01).The result shows: what add 3% and 6% mass fraction in feed is that the Chinese herbal formula of main component all can strengthen the immunity of bolti with the cordate houttuynia, wherein best with the effect of adding 6% mass fraction.
6, above-mentioned embodiment illustrates that compound Chinese herbal medicine additive provided by the invention can effectively improve Tilapia mossambica immunity, strengthens the ability that body is removed foreign matter, thereby improves the premunition of Tilapia mossambica.
Claims (3)
1. Chinese herbal formula that improves Tilapia mossambica immunity, the drug ingedient that it is characterized in that it is pure Chinese herbal medicine, various medicinal herb componentses are respectively: cordate houttuynia is 40%, the root of large-flowered skullcap is 5%, Radix Isatidis is 5%, the capsule of weeping forsythia is 5%, honeysuckle is 10%, Radix Glycyrrhizae is 5%, rheum officinale is 5%, the Radix Astragali is 10%, when be classified as 5%, hawthorn is 10%, each component of Chinese herbal medicine is made the ultra micro powder earlier, mixes by above-mentioned mass fraction proportioning again.
2. tilapia feed that improves the Chinese herbal formula of Tilapia mossambica immunity, it is characterized in that improving the 3%-6% of tilapia feed of Chinese herbal formula of Tilapia mossambica immunity for improving the Chinese herbal formula of Tilapia mossambica immunity, this compound Chinese herb composition and weight proportion are: cordate houttuynia is 40%, the root of large-flowered skullcap is 5%, Radix Isatidis is 5%, the capsule of weeping forsythia is 5%, honeysuckle is 10%, Radix Glycyrrhizae is 5%, rheum officinale is 5%, the Radix Astragali is 10%, when be classified as 5%, hawthorn is 10%;
Basis tilapia feed 94%-97% is to improve the ultra micro powder of the Chinese herbal formula of Tilapia mossambica immunity, to be mixed in the basic tilapia feed by above-mentioned weight proportion, to make the pellet that diameter is 3-5mm behind the mixing, to put in storage after the drying.
3. according to the described a kind of tilapia feed that improves the Chinese herbal formula of Tilapia mossambica immunity of claim 2, it is characterized in that improving the Chinese herbal formula 6% of Tilapia mossambica immunity;
Basis tilapia feed 94% is to improve the ultra micro powder of the Chinese herbal formula of Tilapia mossambica immunity, to be mixed in the basic tilapia feed by above-mentioned weight proportion, to make the pellet that diameter is 3-5mm behind the mixing, to put in storage after the drying.
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