CN103734537B - A kind of method of anti-Streptococcus iniae infection ability when improving gift tilapia Heat-temperature stress - Google Patents
A kind of method of anti-Streptococcus iniae infection ability when improving gift tilapia Heat-temperature stress Download PDFInfo
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Abstract
The present invention discloses a kind of method of anti-Streptococcus iniae infection ability when improving gift tilapia Heat-temperature stress, comprises basal diet preparation, the preparation of compound Chinese herb formula and feedstuff feeding step.Immune indexes when effectively can improve gift tilapia Heat-temperature stress by the method, reduces hepar damnification, can improve the anti-Streptococcus iniae infection ability of gift tilapia simultaneously, reduces incidence probability, increases the survival rate after infecting Streptococcus iniae.Several medicinal herb componentses involved in the present invention are drawn materials comparatively easy, and relative low price, production technology is simple.The basal diet palatability of adding compound Chinese herb is better, and after Tilapia mossambica picked-up, drug disposition remains less, meets the requirement of green health aquatic products.
Description
Technical field
This invention belongs to the feed formula technology of aquaculture, is specifically related to a kind of feed formulation and the feeding method that can improve gift tilapia anti-Streptococcus iniae infection ability when Heat-temperature stress.
Background technology
Streptococcus is widely distributed at occurring in nature, be prevalent in water, animal body surface, urogenital tract and emulsion, all have pathogenic to mammal, reptile, amphibian and fish, be a kind of people-beast-fish ill cause of disease altogether, cause serious economic loss to domestic animal and culture fishery.
Streptococcus optimum growth temperature is 33 DEG C-37 DEG C, and therefore, under the environment of summer high temperature, streptococcosis very easily breaks out.Tilapia mossambica belongs to subtropical zone fish, and suitable growth temperature is 29-32 DEG C.In recent years, along with the aggravation of global warming, in high water temperature season in summer, water temperature reaches 35 DEG C-37 DEG C.Especially summer in 2013, drug in some provinces high temperature reaches about 40 DEG C, nearly 1 month of duration, and this causes heavy economic losses to Tilapia mossambica aquaculture.The incidence of disease of Tilapia mossambica streptococcosis is up to 40%, and the death rate after morbidity is close to 100%.The juvenile fish of streptococcosis more than chief threat 100g, to the adult fish of about 500g, is mainly propagated in a horizontal manner, and body surface has damage or falls ill by the fish easy infection that environmental stress is excessive.According to incompletely statistics, streptococcosis gives the loss that causes of Chinese Tilapia mossambica industry every year up to multi-million dollar.Therefore, how to slow down the reaction of Tilapia mossambica Heat-temperature stress, strengthen immune response, thus the object reaching raising streptococcus infection ability becomes our current problem in the urgent need to address.
Summary of the invention
Anti-Streptococcus iniae infection ability during in order to strengthen gift tilapia Heat-temperature stress, improves the metainfective survival rate of Streptococcus iniae.The invention provides a kind of feed formulation and the feeding method that improve gift tilapia anti-Streptococcus iniae infection ability when Heat-temperature stress, effectively can strengthen the immune protective efficiency under Heat-temperature stress, reduce Streptococcus iniae and infect probability.Meanwhile, the compound Chinese herb formula used belongs to environmental protection preparation, and fish body remains and less to the negative interaction of environment, meets the requirement of green health aquatic products.
The present invention for achieving the above object, takes following technical scheme.
Strengthen immune response during gift tilapia Heat-temperature stress, improve anti-Streptococcus iniae infection ability.Main design idea and routine analyzer comprise the following steps:
One is basal diet preparation, and according to the basal diet of the Different growth phases preparation Different Nutrition composition of gift tilapia, when body weight is between 50g-200g, preparation protein level is 31% ~ 35%, and fat level is the basal diet of 6% ~ 10%; When body weight is higher than 200g, preparation protein level is about 26% ~ 29%, and fat level is the basal diet of 5% ~ 8%.
Two is preparations that compound Chinese herb is filled a prescription, and component and the percetage by weight of compound Chinese herb are respectively (based on 100g compound Chinese herb): aloe-emodin 20g, honeysuckle 15g, wilsonii 15g, chrysanthemum 15g, mint extract 10g, cordate houttuynia 15g, hawthorn 10g.After compound Chinese herb oven dry, grinding, carry out proportioning according to aforementioned proportion.Add the principle of 2g ~ 3.5g according to every 100g basal diet, be mixed with the pharmaceutical feed of 2% ~ 2.5% or 3% ~ 3.5% liang of group compound Chinese herb.
Three is feeding methods, before summer high temperature arrives, first throws something and feeds containing the basal diet 1 month of 3% ~ 3.5% compound Chinese herb, carries out gift tilapia reinforced cultivating.The basal diet of throwing something and feeding containing 2% ~ 2.5% compound Chinese herb is continued at high temperature season.
Immune indexes when effectively can improve gift tilapia Heat-temperature stress by the method, reduces hepar damnification, can improve the anti-Streptococcus iniae infection ability of gift tilapia simultaneously, reduces incidence probability, increases the survival rate after infecting Streptococcus iniae.Several medicinal herb componentses involved in the present invention are drawn materials comparatively easy, and relative low price, production technology is simple.The basal diet palatability of adding compound Chinese herb is better, and after Tilapia mossambica picked-up, drug disposition remains less, meets the requirement of green health aquatic products.
Detailed description of the invention
One, the preparation of basal diet, according to the basal diet of the Different growth phases preparation Different Nutrition composition of gift tilapia, when body weight is between 50g-200g, preparation protein level is about 33%, and fat level is the basal diet of 8%; When body weight is higher than 200g, preparation protein level is about 28%, and fat level is the basal diet of 7%.
Two, the preparation of compound Chinese herb formula, component and the percetage by weight of compound Chinese herb are respectively (based on 100g compound Chinese herb): aloe-emodin 20g, honeysuckle 15g, wilsonii 15g, chrysanthemum 15g, mint extract 10g, cordate houttuynia 15g, hawthorn 10g.After compound Chinese herb oven dry, grinding, carry out proportioning according to aforementioned proportion.Add the principle of 2g or 3g according to every 100g basal diet, be mixed with the pharmaceutical feed of 2% or 3% compound Chinese herb.
Three, feeding method, mid or late April is to mid or late May, start the basal diet feed 1 month (selecting different basal diets as carrier according to body size) of throwing something and feeding containing 3% compound Chinese herb, carry out gift tilapia reinforced cultivating, throw something and feed every day 3 times (7:30,11:30 and 16:00), and feeding volume is the 4%-6% of body weight.Start June to enter high temperature season, continue the basal diet (selecting different basal diets as carrier according to body size) of throwing something and feeding containing 2% compound Chinese herb, if fine, throw something and feed every day 2 times (8:00 and 15:00), and feeding volume is the 3%-5% of body weight; Cloudy day, rainy day or muggy weather, then throw something and feed 1 time or do not throw something and feed.
Table 1 control group and the routine blood indexes analysis after 48 hours of compound Chinese herb processed group 36 DEG C of Heat-temperature stress
36 DEG C of Heat-temperature stress are after 48 hours, and in the gift tilapia blood of compound Chinese herb processed group, red blood cell and quantity of leucocyte, HC and packed cell volume are apparently higher than the control group not adding compound Chinese herb.
Table 2 control group and serum biochemistry and the immune indexes analysis after 48 hours of compound Chinese herb processed group 36 DEG C of Heat-temperature stress
36 DEG C of Heat-temperature stress are after 48 hours, and the gift tilapia liver of compound Chinese herb processed group stress reduce, and serum glutamic pyruvic transminase and glutamic-oxalacetic transaminease vigor are lower than control group; Immunologic function increases, and shows as HDL-C, lysozyme, Complement C_3 and superoxide dismutase activity higher than control group.Alkaline phosphatase is also higher than control group simultaneously, and glucose and low density cholesterol are starkly lower than control group.
Table 3 control group and compound Chinese herb processed group 36 DEG C of Heat-temperature stress liver Antioxidant index after 48 hours
36 DEG C of Heat-temperature stress are after 48 hours, the gift tilapia liver anti-oxidation stress ability of compound Chinese herb processed group strengthens, superoxide dismutase, catalase, glutathione and glutathione peroxidase activity are all apparently higher than control group, and MDA level is lower than control group.
36 DEG C of Heat-temperature stress are after 48 hours, control group with add compound Chinese herb processed group and choose 30 tail fishes respectively and carry out Streptococcus iniae infection, with 10 tails/bucket, often group arrange 3 parallel.Lumbar injection 7.63 × 10
6the Streptococcus iniae bacterium liquid of CPU/ml, carries out lumbar injection according to the principle of every 100g body weight injection 0.15ml.Infect the cumulative mortality after 192 hours and see the following form 4.
Table 4 control group compares with the death rate after compound Chinese herb processed group gift tilapia artificial challenge Streptococcus iniae 192h
Through process of the present invention, the immunity of gift tilapia when Heat-temperature stress significantly improves, and liver anti-oxidation stress ability strengthens, and the cumulative mortality of Heat-temperature stress postoperative infection Streptococcus iniae reduces 29.22%.In April, 2013, choose 5 Tilapia mossambica cultivation bases in gaozhou,guangdong city, Shaoguan and Zhanjiang City and Nanning and Beihai, throw something and feed according to the method in the present invention.In 8-10 month, in streptococcosis season occurred frequently, the gift tilapia incidence of disease adding compound Chinese herb processed group reduces 39.53%, and after morbidity, the survival rate of Tilapia mossambica improves 21.54%.
Claims (1)
1. improve a method for anti-Streptococcus iniae infection ability during gift tilapia Heat-temperature stress, comprise the steps:
(1) basal diet preparation, according to the basal diet of the Different growth phases preparation Different Nutrition composition of gift tilapia, when body weight is between 50g-200g, preparation protein level is 31% ~ 35%, and fat level is the basal diet of 6% ~ 10%; When body weight is higher than 200g, preparation protein level is 26% ~ 29%, and fat level is the basal diet of 5% ~ 8%;
(2) compound Chinese herb formula preparation, in 100g compound Chinese herb, the weight of each component respectively: aloe-emodin 20g, honeysuckle 15g, wilsonii 15g, chrysanthemum 15g, mint extract 10g, cordate houttuynia 15g, hawthorn 10g; After compound Chinese herb oven dry, grinding, carry out proportioning according to aforementioned proportion, add the principle of 2g ~ 3.5g according to every 100g basal diet, be mixed with the pharmaceutical feed of 2% ~ 2.5% or 3% ~ 3.5% liang of group compound Chinese herb;
(3) feeding method, before summer high temperature arrives, first throws something and feeds containing the basal diet 1 month of 3% ~ 3.5% compound Chinese herb, carries out gift tilapia reinforced cultivating; The basal diet of throwing something and feeding containing 2% ~ 2.5% compound Chinese herb is continued at high temperature season.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104286595A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Juvenile tilapia feed |
| CN105166491A (en) * | 2015-09-03 | 2015-12-23 | 哈尔滨泉兴科技有限公司 | Tilapia feed and preparation method therefor |
| CN106172145B (en) * | 2016-09-13 | 2019-04-19 | 中国水产科学研究院淡水渔业研究中心 | A method of improving the anti-Streptococcus iniae infection of gift tilapia of high-density breeding |
| CN106172144B (en) * | 2016-09-13 | 2019-04-16 | 中国水产科学研究院淡水渔业研究中心 | A method of promoting gift tilapia seed uniform growth and immunologic facilitation |
| CN106754488A (en) * | 2016-11-30 | 2017-05-31 | 广西南宁市武鸣明山红农业科技开发有限公司 | A kind of Streptococcus iniae disease biological agent and preparation method and application |
| CN110004237A (en) * | 2019-06-06 | 2019-07-12 | 鲁东大学 | A kind of appraisement system and evaluation method of hippocampus response |
| CN115553234B (en) * | 2022-03-17 | 2023-09-29 | 南通龙洋水产有限公司 | Breeding method of broad-spectrum antibacterial tilapia fine variety |
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| CN101313731B (en) * | 2008-07-17 | 2012-02-22 | 北京中农博乐科技开发有限公司 | Anti-stress feedstuff additive |
| CN102150757B (en) * | 2011-02-14 | 2013-06-26 | 北京伟嘉人生物技术有限公司 | Aquatic product feed additive composition, preparation method and application thereof |
| CN102763782B (en) * | 2012-08-01 | 2013-08-28 | 中国水产科学研究院珠江水产研究所 | Compound Chinese herbal medicine preparation for improving tilapia immunity |
| CN103392940A (en) * | 2013-08-06 | 2013-11-20 | 中国水产科学研究院淡水渔业研究中心 | Feed for improving streptococcal disease resistance of tilapia and feeding method thereof |
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