CN106754488A - A kind of Streptococcus iniae disease biological agent and preparation method and application - Google Patents
A kind of Streptococcus iniae disease biological agent and preparation method and application Download PDFInfo
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- CN106754488A CN106754488A CN201611081617.6A CN201611081617A CN106754488A CN 106754488 A CN106754488 A CN 106754488A CN 201611081617 A CN201611081617 A CN 201611081617A CN 106754488 A CN106754488 A CN 106754488A
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Abstract
The invention discloses a kind of Streptococcus iniae disease biological agent and preparation method and application, belong to Tilapia mossambica Streptococcus iniae disease Prevention Technique field, ullage disappears the Streptococcus iniae disease biological agent by fermentation, prepared by culture medium, culture medium disappears in fact, connect strain, strain fermentation, concentration, drying, crushing, sieving are made.Streptococcus iniae disease biological agent is added in feed of the invention, can effectively strengthen the immunity and anti-stress ability of Tilapia mossambica, reduce the death rate that Tilapia mossambica suffers from Streptococcus iniae disease, improve cure rate, improve productivity effect.
Description
【Technical field】
The invention belongs to Tilapia mossambica Streptococcus iniae disease Prevention Technique field, and in particular to a kind of Streptococcus iniae diease occurrence thing
Preparation and preparation method and application.
【Background technology】
In the production process of biological feedstuff, addition is common insect albumen powder, the production work of common insect albumen powder
It is that worm's ovum is cultivated as culture with wheat bran, green vegetables in skill, after worm's ovum is grown up to pure worm, to pure worm fried dry or microwave
Baking oven is done, and is then comminuted into worm powder, then worm powder is mixed with culture or fermented bean dregs or general proteins raw material, forms common
Insect albumen powder, in the production technology, because the culture cultivated worm's ovum is wheat bran, green vegetables, therefore the pure worm for growing up to
Composition in be free of antibacterial peptide and saccharomycete, therefore effect without enhance immunity；Due to using the drying to pure worm fried dry
Mode, easily makes protein denaturation, so as to destroy due nutritional ingredient；Due to crushing to form worm powder pure worm, do not make water
Solution treatment, composition exists in the form of the protein of intrinsic macromolecular or in the form of polypeptide, therefore is not easy to be absorbed.
Raising of the phagostimulant to Tilapia mossambica has obvious and positive biological action, can improve the attractant of feed and fit
Mouth property, improves food conversion ratio, and then improves the production performance of Tilapia mossambica comprehensively, such as increases feed intake, raising daily gain, improves
Meat quality etc..One side phagostimulant can cover the bad smell in feed by special odor, stimulate the sensation of Tilapia mossambica
Organ, to reach the purpose for promoting Tilapia mossambica to ingest, accelerate Growth Op Tilapia；On the other hand, Tilapia mossambica is induced by phagostimulant
Ingest, shorten the time of ingesting, improve the utilization rate of feed.It is flavouring agent or sweet taste that phagostimulant in the market is most of
Agent, effect is more single, can only form single stimulation to the smell of Tilapia mossambica or vision or the sense of taste etc.；And some flavouring agents or
The constituent of sweetener comes from chemically synthesized product, produces medicament residue, toxic and side effect obvious easily in Tilapia mossambica body.Cause
This, how by these single Attractants be compounded to form effect substantially, steady quality and economical and practical phagostimulant is trend
Place.
Streptococcus is widely present in nature, people and animal, causes various purulent inflammations, scarlet fever, erysipelas, neonate
Septicemia, meningitis, puerperal fever and streptococcus allergic disease etc..Aquiculture animal can also infect streptococcus generation
Streptococcosis, causes massive mortality, causes great economic loss to World Aquaculture every year.China is big aquaculture
State, every year because streptococcosis is to about 1,000,000,000 dollars of the direct economic loss caused by aquaculture.Tilapia mossambica be the important torrid zone and
Subtropical zone aquiculture animal, is also one of main object of streptococcal infection.2001 start, and Chinese Rofe fish culture is successively
The infection of streptococcosis is occurred in that, and the high mortality of 30%-50% is caused in indivedual plants.2009-2013 continuous 5
Year, streptococcosis in Chinese Rofe fish culture large area outbreak of epidemic, diseased region expands year by year, morbidity and mortality year by year
It is incremented by, susceptible Tilapia mossambica specification limit expands year by year.By the average annual Production rate of China Tilapia mossambica, estimate annual streptococcosis to me
Just up to 400,000,000 dollars of the direct economic loss that state's Tilapia mossambica industry is caused.Breaking out for disease is asked with the product safety brought therewith
Topic has turned into the bottleneck of restriction Tilapia mossambica industry development.Lack a set of effective scheme at present and prevent and treat the disease, medicine
Thing can only play the complementary effect of symptom management in early stage, but with resistance and drug-fast generation and accumulation, and the disease shows
Shape is almost " pasting medical help to prevent ".
With the increase of market demand, the outburst of various diseases causes tremendous influence to Tilapia mossambica industry, therefore
Effective prevention and treatment of Tilapia mossambica disease, particularly streptococcosis how are effectively prevented and treated, yield is improved, industry size is increased,
Development Tilapia mossambica healthy aquaculture technology has had very big necessity and urgency.
【The content of the invention】
The technical problem to be solved in the present invention is to provide a kind of Streptococcus iniae disease biological agent and preparation method thereof and answers
With, it is difficult to prevent and treat to solve Tilapia mossambica streptococcosis, the death rate is high after being ill, cure rate is low to cause Tilapia mossambica to suffer from Streptococcus iniae
Problem.Streptococcus iniae disease biological agent is added in feed of the invention, can effectively strengthen the immunity and resisting stress of Tilapia mossambica
Ability, reduces the death rate that Tilapia mossambica suffers from Streptococcus iniae disease, improves cure rate, improves productivity effect.
In order to solve the above technical problems, the present invention uses following technical scheme：
A kind of Streptococcus iniae disease biological agent, it is characterised in that be that ullage disappears by fermentation, prepared by culture medium, culture
Base disappears in fact, connect strain, strain fermentation, concentration, drying, crushing, sieving are made.
The present invention also provides a kind of preparation method of Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：Peptone 160-200 parts, 100-120 parts of Yeast diffusion juice powder, beef extract 40-60 parts, glucose 15-25 parts, Ma Ling
Potato leach 30-50 part of powder, agar powder 20-40 parts, 2-4 parts of trace element, water 1400-2600 parts, it is described micro- with weight
Part it is unit, including following raw material：Zn-ef ficiency 4-9 parts, selenium element 3-6 parts, I 2-4 parts, copper 1-3 parts, manganese element
2-3 parts；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, nitrifier, actinomyces, photosynthetic bacteria, temperature be 32-36 DEG C, rotating speed be 200-220r/min under enter
Row strain fermentation 50-80h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitre
It is 5-7 to change bacterium, actinomyces, the weight ratio of photosynthetic bacteria:3-4:2-4:2-3:1-3:1-2；
(E) step (D) completed into the zymotic fluid concentration of strain fermentation, dried to water content≤3%, it is size-reduced to cross 100-
300 mesh sieve, are obtained Streptococcus iniae disease biological agent.
The present invention also provides a kind of application of Streptococcus iniae disease biological agent, and the Streptococcus iniae disease biological agent should
In for tilapia feed, the tilapia feed is in units of weight portion, including following raw material：Albumen powder 30-50 parts, soybean
Dregs of rice 10-18 parts, peanut meal 6-12 parts, corn 8-14 parts, Cottonseed Meal 4-7 parts, rapeseed dregs 5-8 parts, Strong flour 10-16 parts, dimension
Sick plain 1.8-3 parts, -2 parts of amino acid/11,1-1.8 parts of trace element, vegetable oil 5-8 parts, phagostimulant 1-2 parts, Streptococcus iniae
Biological agent 0.6-0.9 parts.
Preferably, the vitamin is composed of the following components in units of weight portion：Raw 0.8-1.2 parts of element A, vitamin
B10.6-1 parts, vitamin C 0.4-0.8 parts.
Preferably, the amino acid is composed of the following components in units of weight portion：Phenylalanine 0.5-0.8 parts, paddy ammonia
It is sour 0.3-0.7 parts, serine 0.2-0.5 parts.
Preferably, the trace element is composed of the following components in units of weight portion：Copper 0.05-0.1 parts, manganese
Element 0.05-0.1 parts, chromium 0.05-0.1 parts, ferro element 0.2-0.45 parts, Zn-ef ficiency 0.2-0.45 parts, selenium element 0.25-
0.3 part, I 0.2-0.3 parts.
Preferably, the vegetable oil includes the one kind or many in peanut oil, soybean oil, linseed oil, castor oil, rape seed oil
Kind.
Preferably, the phagostimulant, in units of weight portion, including following raw material：Anise 6-18 parts, matrimony vine 5-15
Part, Poria cocos 4-12 parts, peppermint 3-10 parts, hawthorn 4-8 parts, purslane 2-6 parts, garlic 4-6 parts, Chinese cassia tree 2-4 parts, dimethyl-β-
DMPT 20-30 parts, glycine betaine 15-23 parts, nucleotides 5-10 parts, sweetener 0.6-1 parts, flavouring agent 0.2-0.5 parts, tart flavour
Agent 1-3 parts, 0.4-0.8 parts, tasty agents 0.3-0.6 parts of pungent agent；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
Preferably, the preparation method of the phagostimulant, comprises the following steps：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to≤8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 150-300 parts of water, is 1 in solid-liquid ratio:12-14 (W/V), microwave power is 170-200W, and Extracting temperature is
89-94 DEG C, extraction time is 1.2-1.5h, then filter residue, concentration, dry to water content≤3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained.
Preferably, the preparation method of the tilapia feed, comprises the following steps：
S1：Soybean Meal, peanut meal, corn, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, the sieve of 100-300 mesh is crossed
After be well mixed, be obtained homogeneous mixture；
S2：To addition albumen powder, Strong flour, vitamin, amino acid, micro unit in homogeneous mixture obtained in step S1
Element, vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 100-160 parts of water, are well mixed again, and wet feeding is obtained
Material；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：Agricultural waste material 40-48 parts, vegetables slag 30-40 parts, water 45-52
Part, bacteria leaven 8-16 parts, cavings 12-22 parts；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：By the pure worm 326-352g of step (2), by material ratio 1:3.5-3.8 (W/V) adds water grinding,
Sterilize 24-26min at 76-78 DEG C, is cooled to 34-40 DEG C, and slurries are obtained；
(4) slurry hydrolysis：To cysteine proteinase 0.26-0.3g is added in slurries obtained in step (3), pH value is adjusted
It is 5.6-6,37-41h is hydrolyzed at 46-50 DEG C, then sterilize 25-30min at 70-73 DEG C, centrifugal filtration, hydrolysis is obtained
Liquid；
(5) hydrolyzate ultrafiltration：Hydrolyzate obtained in step (4) is used into the hollow fibre that molecular cut off is 7000-8000
Dimension film ultrafiltration, is obtained ultrafiltrate；
(6) dry, crush, sieve：Ultrafiltrate obtained in step (5) is carried out into freeze-drying, 200-300 mesh is crossed after crushing
Sieve, is obtained albumen powder；
S3：Moist forage obtained in step S2 is made the particle of diameter 0.1-0.3cm and the baking in the case where temperature is for 90-105 DEG C
Do to moisture≤3.6%, be finally cooled to 25-30 DEG C, tilapia feed is obtained.
The invention has the advantages that：
Streptococcus iniae disease biological agent is added in feed of the invention, can effectively be strengthened the immunity of Tilapia mossambica and be resisted and answer
The ability of swashing, reduces the death rate that Tilapia mossambica suffers from Streptococcus iniae disease, improves cure rate, improves productivity effect.
【Specific embodiment】
For ease of more fully understanding the present invention, it is illustrated by following examples, these embodiments belong to of the invention
Protection domain, but do not limit the scope of the invention.
In embodiment, the tilapia feed, in units of weight portion, including following raw material：Albumen powder 30-50 parts,
Soybean Meal 10-18 parts, peanut meal 6-12 parts, corn 8-14 parts, Cottonseed Meal 4-7 parts, rapeseed dregs 5-8 parts, Strong flour 10-16
Part, vitamin 1.8-3 parts, -2 parts of amino acid/11,1-1.8 parts of trace element, vegetable oil 5-8 parts, phagostimulant 1-2 parts, dolphin hammer
Bacterium 0.6-0.9 parts of biological agent of disease.
The vitamin is composed of the following components in units of weight portion：Raw 0.8-1.2 parts of element A, vitamin B1 0.6-
1 part, vitamin C 0.4-0.8 parts.
The amino acid is composed of the following components in units of weight portion：Phenylalanine 0.5-0.8 parts, glutamic acid 0.3-
0.7 part, serine 0.2-0.5 parts.
The trace element is composed of the following components in units of weight portion：Copper 0.05-0.1 parts, manganese element
0.05-0.1 parts, chromium 0.05-0.1 parts, ferro element 0.2-0.45 parts, Zn-ef ficiency 0.2-0.45 parts, selenium element 0.25-0.3
Part, I 0.2-0.3 parts.
The vegetable oil includes one or more in peanut oil, soybean oil, linseed oil, castor oil, rape seed oil.
The phagostimulant, in units of weight portion, including following raw material：Anise 6-18 parts, matrimony vine 5-15 parts, Poria cocos 4-
12 parts, peppermint 3-10 parts, hawthorn 4-8 parts, purslane 2-6 parts, garlic 4-6 parts, Chinese cassia tree 2-4 parts, dimethyl-β-DMPT
20-30 parts, glycine betaine 15-23 parts, nucleotides 5-10 parts, sweetener 0.6-1 parts, flavouring agent 0.2-0.5 parts, acid 1-3 parts,
0.4-0.8 parts, tasty agents 0.3-0.6 parts of pungent agent；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the tilapia feed, comprises the following steps：
S1：Soybean Meal, peanut meal, corn, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, the sieve of 100-300 mesh is crossed
After be well mixed, be obtained homogeneous mixture；
S2：To addition albumen powder, Strong flour, vitamin, amino acid, micro unit in homogeneous mixture obtained in step S1
Element, vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 100-160 parts of water, are well mixed again, and wet feeding is obtained
Material；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：Agricultural waste material 40-48 parts, vegetables slag 30-40 parts, water 45-52
Part, bacteria leaven 8-16 parts, cavings 12-22 parts；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：By the pure worm 326-352g of step (2), by material ratio 1:3.5-3.8 (W/V) adds water grinding,
Sterilize 24-26min at 76-78 DEG C, is cooled to 34-40 DEG C, and slurries are obtained；
(4) slurry hydrolysis：To cysteine proteinase 0.26-0.3g is added in slurries obtained in step (3), pH value is adjusted
It is 5.6-6,37-41h is hydrolyzed at 46-50 DEG C, then sterilize 25-30min at 70-73 DEG C, centrifugal filtration, hydrolysis is obtained
Liquid；
(5) hydrolyzate ultrafiltration：Hydrolyzate obtained in step (4) is used into the hollow fibre that molecular cut off is 7000-8000
Dimension film ultrafiltration, is obtained ultrafiltrate；
(6) dry, crush, sieve：Ultrafiltrate obtained in step (5) is carried out into freeze-drying, 200-300 mesh is crossed after crushing
Sieve, is obtained albumen powder；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to≤8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 150-300 parts of water, is 1 in solid-liquid ratio:12-14 (W/V), microwave power is 170-200W, and Extracting temperature is
89-94 DEG C, extraction time is 1.2-1.5h, then filter residue, concentration, dry to water content≤3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：Peptone 160-200 parts, 100-120 parts of Yeast diffusion juice powder, beef extract 40-60 parts, glucose 15-25 parts, Ma Ling
Potato leach 30-50 part of powder, agar powder 20-40 parts, 2-4 parts of trace element, water 1400-2600 parts, it is described micro- with weight
Part it is unit, including following raw material：Zn-ef ficiency 4-9 parts, selenium element 3-6 parts, I 2-4 parts, copper 1-3 parts, manganese element
2-3 parts；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, nitrifier, actinomyces, photosynthetic bacteria, temperature be 32-36 DEG C, rotating speed be 200-220r/min under enter
Row strain fermentation 50-80h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitre
It is 5-7 to change bacterium, actinomyces, the weight ratio of photosynthetic bacteria:3-4:2-4:2-3:1-3:1-2；
(E) step (D) completed into the zymotic fluid concentration of strain fermentation, dried to water content≤3%, it is size-reduced to cross 100-
300 mesh sieve, are obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 is made the particle of diameter 0.1-0.3cm and the baking in the case where temperature is for 90-105 DEG C
Do to moisture≤3.6%, be finally cooled to 25-30 DEG C, tilapia feed is obtained.
It is illustrated below by more specifically embodiment.
Embodiment 1
A kind of tilapia feed, in units of weight portion, including following raw material：40 parts of albumen powder, 15 parts of Soybean Meal, peanut
9 parts of the dregs of rice, 12 parts of corn, 6 parts of Cottonseed Meal, 7 parts of rapeseed dregs, 13 parts of Strong flour, 2.5 parts of vitamin, amino acid/11 .5 parts, it is micro
1.4 parts of element, 7 parts of vegetable oil, 1.6 parts of phagostimulant, Streptococcus iniae 0.7 part of biological agent of disease.
The vitamin is composed of the following components in units of weight portion：Raw 1 part of element A, vitamin B10.8 part, dimension life
0.7 part of plain C.
The amino acid is composed of the following components in units of weight portion：0.7 part of phenylalanine, glutamic acid 0.5 part, thread
0.3 part of propylhomoserin.
The trace element is composed of the following components in units of weight portion：0.075 part of copper, manganese element 0.075
Part, 1 part of chromium, 0.3 part of ferro element, 0.3 part of Zn-ef ficiency, 0.3 part of selenium element, 0.25 part of I.
The vegetable oil is peanut oil.
The phagostimulant, in units of weight portion, including following raw material：It is 12 parts of anise, 10 parts of matrimony vine, 8 parts of Poria cocos, thin
7 parts of lotus, 6 parts of hawthorn, 4 parts of purslane, 10 parts of garlic, 3 parts of Chinese cassia tree, 25 parts of dimethyl-β-DMPT, 20 parts of glycine betaine, core
7 parts of thuja acid, 0.8 part of sweetener, 0.4 part of flavouring agent, 2 parts of acid, 0.6 part of pungent agent, 0.5 part of tasty agents；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the tilapia feed, comprises the following steps：
S1：Soybean Meal, peanut meal, corn, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 200 mesh
Close uniform, homogeneous mixture is obtained；
S2：To addition albumen powder, Strong flour, vitamin, amino acid, micro unit in homogeneous mixture obtained in step S1
Element, vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 140 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：45 parts of agricultural waste material, 35 parts of vegetables slag, 50 parts of water, strain fermentation
13 parts of agent, 18 parts of cavings；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：By the pure worm 340g of step (2), by material ratio 1:3.6 (W/V) add water grinding, are gone out at 77 DEG C
Bacterium 25min, is cooled to 38 DEG C, and slurries are obtained；
(4) slurry hydrolysis：To cysteine proteinase 0.28g is added in slurries obtained in step (3), adjusting pH value is
5.8,39h is hydrolyzed at 48 DEG C, then sterilize 27min at 72 DEG C, centrifugal filtration, and hydrolyzate is obtained；
(5) hydrolyzate ultrafiltration：Hydrolyzate obtained in step (4) is super using the hollow-fibre membrane that molecular cut off is 7500
Filter, is obtained ultrafiltrate；
(6) dry, crush, sieve：Ultrafiltrate obtained in step (5) is carried out into freeze-drying, 250 mesh sieves is crossed after crushing,
Albumen powder is obtained；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to 8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 250 parts of water, is 1 in solid-liquid ratio:13 (W/V), microwave power is 185W, and Extracting temperature is 92 DEG C, during extraction
Between be 1.4h, then filter residue, concentration, to dry to water content be 3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：180 parts of peptone, 110 parts of Yeast diffusion juice powder, 50 parts of beef extract, 20 parts of glucose, potato leach 40 parts of powder,
30 parts of agar powder, micro- 3 parts, 2000 parts of water, the trace element is in units of weight portion, including following raw material：Zinc unit
7 parts of element, 5 parts of selenium element, 3 parts of I, 2 parts of copper, 2.5 parts of manganese element；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, nitrifier, actinomyces, photosynthetic bacteria, temperature be 35 DEG C, rotating speed be 210r/min under carry out strain hair
Ferment 65h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces,
The weight ratio of photosynthetic bacteria is 6:3.5:3:2.5:2:1.5；
(E) it is 3%, the mesh of size-reduced mistake 200 to concentrate the zymotic fluid that step (D) completes strain fermentation, dry to water content
Sieve, is obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1-0.3cm and temperature be 100 DEG C at dry to
Moisture is 3.6%, is finally cooled to 28 DEG C, and tilapia feed is obtained.
Embodiment 2
A kind of tilapia feed, in units of weight portion, including following raw material：30 parts of albumen powder, 10 parts of Soybean Meal, peanut
6 parts of the dregs of rice, 8 parts of corn, 4 parts of Cottonseed Meal, 5 parts of rapeseed dregs, 10 parts of Strong flour, 1.8 parts of vitamin, amino acid/11 part, trace element
1 part, 5 parts of vegetable oil, 1 part of phagostimulant, Streptococcus iniae disease 0.6 part of biological agent.
The vitamin is composed of the following components in units of weight portion：Raw 0.8 part of element A, vitamin B10.6 part, dimension
Raw 0.4 part of element C.
The amino acid is composed of the following components in units of weight portion：0.5 part of phenylalanine, glutamic acid 0.3 part, thread
0.2 part of propylhomoserin.
The trace element is composed of the following components in units of weight portion：0.05 part of copper, 0.05 part of manganese element,
0.05 part of chromium, 0.2 part of ferro element, 0.2 part of Zn-ef ficiency, 0.25 part of selenium element, 0.2 part of I.
The vegetable oil is soybean oil.
The phagostimulant, in units of weight portion, including following raw material：6 parts of anise, 5 parts of matrimony vine, 4 parts of Poria cocos, peppermint
3 parts, 4 parts of hawthorn, 2 parts of purslane, 4 parts of garlic, 2 parts of Chinese cassia tree, 20 parts of dimethyl-β-DMPT, 15 parts of glycine betaine, nucleotides
5 parts, 0.6 part of sweetener, 0.2 part of flavouring agent, 1 part of acid, 0.4 part of pungent agent, 0.3 part of tasty agents；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the tilapia feed, comprises the following steps：
S1：Soybean Meal, peanut meal, corn, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 100 mesh
Close uniform, homogeneous mixture is obtained；
S2：To addition albumen powder, Strong flour, vitamin, amino acid, micro unit in homogeneous mixture obtained in step S1
Element, vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 100 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：40 parts of agricultural waste material, 30 parts of vegetables slag, 45 parts of water, strain fermentation
8 parts of agent, 12 parts of cavings；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：By the pure worm 326g of step (2), by material ratio 1:3.5 (W/V) add water grinding, are gone out at 76 DEG C
Bacterium 26min, is cooled to 34 DEG C, and slurries are obtained；
(4) slurry hydrolysis：To cysteine proteinase 0.26g is added in slurries obtained in step (3), adjusting pH value is
5.6,41h is hydrolyzed at 46 DEG C, then sterilize 30min at 70 DEG C, centrifugal filtration, and hydrolyzate is obtained；
(5) hydrolyzate ultrafiltration：Hydrolyzate obtained in step (4) is super using the hollow-fibre membrane that molecular cut off is 7000
Filter, is obtained ultrafiltrate；
(6) dry, crush, sieve：Ultrafiltrate obtained in step (5) is carried out into freeze-drying, 200 mesh sieves is crossed after crushing,
Albumen powder is obtained；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal most 7.4% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 150 parts of water, is 1 in solid-liquid ratio:12 (W/V), microwave power is 170W, and Extracting temperature is 89 DEG C, during extraction
Between be 1.5h, then filter residue, concentration, to dry to water content be 2.6%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：160 parts of peptone, 100 parts of Yeast diffusion juice powder, 40 parts of beef extract, 15 parts of glucose, potato leach 30 parts of powder,
20 parts of agar powder, micro- 2 parts, 1400 parts of water, the trace element is in units of weight portion, including following raw material：Zinc unit
4 parts of element, 3 parts of selenium element, 2 parts of I, 1 part of copper, 2 parts of manganese element；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, nitrifier, actinomyces, photosynthetic bacteria, temperature be 32 DEG C, rotating speed be 200r/min under carry out strain hair
Ferment 80h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces,
The weight ratio of photosynthetic bacteria is 5:3:2:2:1:1；
(E) it is 2.5%, size-reduced mistake 100 to concentrate the zymotic fluid that step (D) completes strain fermentation, dry to water content
Mesh sieve, is obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1cm and temperature be 90 DEG C at dry to moisture
Content is 3.2%, is finally cooled to 25 DEG C, and tilapia feed is obtained.
Embodiment 3
A kind of tilapia feed, in units of weight portion, including following raw material：50 parts of albumen powder, 18 parts of Soybean Meal, peanut
12 parts of the dregs of rice, 14 parts of corn, 7 parts of Cottonseed Meal, 8 parts of rapeseed dregs, 16 parts of Strong flour, 3 parts of vitamin, 2 parts of amino acid, trace element
1.6 parts, 8 parts of vegetable oil, 2 parts of phagostimulant, Streptococcus iniae disease 0.9 part of biological agent.
The vitamin is composed of the following components in units of weight portion：Raw 1.2 parts of element A, vitamin B11 part, dimension life
0.8 part of plain C.
The amino acid is composed of the following components in units of weight portion：0.8 part of phenylalanine, glutamic acid 0.7 part, thread
0.5 part of propylhomoserin.
The trace element is composed of the following components in units of weight portion：0.1 part of copper, 0.1 part of manganese element, chromium
0.1 part of element, 0.45 part of ferro element, 0.45 part of Zn-ef ficiency, 0.4 part of selenium element, 0.4 part of I.
The vegetable oil is 2 parts of peanut oil, 2 parts of soybean oil, 2 parts of castor oil, 2 parts of rape seed oil.
The phagostimulant, in units of weight portion, including following raw material：18 parts of anise, 15 parts of matrimony vine, 12 parts of Poria cocos,
10 parts of peppermint, 8 parts of hawthorn, 6 parts of purslane, 6 parts of garlic, 4 parts of Chinese cassia tree, 30 parts of dimethyl-β-DMPT, 23 parts of glycine betaine,
10 parts of nucleotides, 1 part of sweetener, 0.5 part of flavouring agent, 3 parts of acid, 0.8 part of pungent agent, 0.6 part of tasty agents；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the tilapia feed, comprises the following steps：
S1：Soybean Meal, peanut meal, corn, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 300 mesh
Close uniform, homogeneous mixture is obtained；
S2：To addition albumen powder, Strong flour, vitamin, amino acid, micro unit in homogeneous mixture obtained in step S1
Element, vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 160 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：48 parts of agricultural waste material, 40 parts of vegetables slag, 52 parts of water, strain fermentation
16 parts of agent, 22 parts of cavings；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：By the pure worm 352g of step (2), by material ratio 1:3.8 (W/V) add water grinding, are gone out at 78 DEG C
Bacterium 24min, is cooled to 40 DEG C, and slurries are obtained；
(4) slurry hydrolysis：To cysteine proteinase 0.3g is added in slurries obtained in step (3), regulation pH value is 6,
37h is hydrolyzed at 50 DEG C, then sterilize 25min at 73 DEG C, centrifugal filtration, hydrolyzate is obtained；
(5) hydrolyzate ultrafiltration：Hydrolyzate obtained in step (4) is super using the hollow-fibre membrane that molecular cut off is 8000
Filter, is obtained ultrafiltrate；
(6) dry, crush, sieve：Ultrafiltrate obtained in step (5) is carried out into freeze-drying, 300 mesh sieves is crossed after crushing,
Albumen powder is obtained；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal most 6.5% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 300 parts of water, is 1 in solid-liquid ratio:14 (W/V), microwave power is 200W, and Extracting temperature is 94 DEG C, during extraction
Between be 1.2h, then filter residue, concentration, to dry to water content be 2.5%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：200 parts of peptone, 120 parts of Yeast diffusion juice powder, 60 parts of beef extract, 25 parts of glucose, potato leach 50 parts of powder,
40 parts of agar powder, micro- 4 parts, 2600 parts of water, the trace element is in units of weight portion, including following raw material：Zinc unit
9 parts of element, 6 parts of selenium element, 4 parts of I, 3 parts of copper, 3 parts of manganese element；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 5min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, nitrifier, actinomyces, photosynthetic bacteria, temperature be 36 DEG C, rotating speed be 220r/min under carry out strain hair
Ferment 50h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces,
The weight ratio of photosynthetic bacteria is 7:4:4:3:3:2；
(E) it is 2.4%, size-reduced mistake 300 to concentrate the zymotic fluid that step (D) completes strain fermentation, dry to water content
Mesh sieve, is obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1-0.3cm and temperature be 105 DEG C at dry to
Moisture is 3.2%, is finally cooled to 30 DEG C, and tilapia feed is obtained.
To verify the effect of tilapia feed of the present invention, by following experimental verification.
From 0.1-12kg, specification it is almost consistent and make a definite diagnosis suffer from Streptococcus iniae disease Tilapia mossambica 360, be randomly divided into
Four groups, i.e. control group, test group 1, test group 2, test group 3, every group is done 3 parallel laboratory tests, every group of Tilapia mossambica 30.Control group
Tilapia mossambica feeding is with albumen powder, Soybean Meal, peanut meal, corn, Cottonseed Meal, rapeseed dregs, Strong flour, vitamin, amino acid, micro-
The basic feed that secondary element, vegetable oil, phagostimulant are configured to, the Tilapia mossambica of test group 1-3 feeds embodiment of the present invention 1-3 respectively
Feed.Continuous feeding 10 days, feeds intake 2 times daily, experimental period 10 days, calculated after terminating Tilapia mossambica average daily gain amount,
Average Day feeding amount, feed coefficient, the death rate and cure rate, as a result see the table below.
As seen from the above table, test group is contrasted with control group, and feed of the invention can be improved suffers from Streptococcus iniae disease Rofe
The feed intake and the speed of growth of fish, the addition of Streptococcus iniae disease biological agent can effectively strengthen the immunity of Tilapia mossambica and resist answers
The ability of swashing, reduces the death rate that Tilapia mossambica suffers from Streptococcus iniae disease, improves cure rate.
The specific implementation of the invention is not to be limited to these illustrations for above content, is led for technology belonging to the present invention
For the those of ordinary skill in domain, without departing from the inventive concept of the premise, some simple deduction or replace can also be made,
The scope of patent protection that the claims of the present invention by being submitted to determine should be all considered as belonging to.
Claims (10)
1. a kind of Streptococcus iniae disease biological agent, it is characterised in that be that ullage disappears by fermentation, prepared by culture medium, culture medium
Disappear in fact, connect strain, strain fermentation, concentration, drying, crushing, sieving and be made.
2. a kind of preparation method of Streptococcus iniae according to claim 1 disease biological agent, it is characterised in that including with
Lower step：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize 30min
Carry out；
(B) to culture medium is added in the biological fermentation tank of step (A), the culture medium is in units of weight portion, including following original
Material：Peptone 160-200 parts, 100-120 parts of Yeast diffusion juice powder, beef extract 40-60 parts, glucose 15-25 parts, potato leaching
Go out powder 30-50 parts, agar powder 20-40 parts, 2-4 parts of trace element, water 1400-2600 parts, the trace element is with weight portion
Unit, including following raw material：Zn-ef ficiency 4-9 parts, selenium element 3-6 parts, I 2-4 parts, copper 1-3 parts, manganese element 2-3
Part；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, pressure be 1.5 kilograms under sterilize
What 3min was carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, nitrifier, actinomyces, photosynthetic bacteria, temperature be 32-36 DEG C, rotating speed be 200-220r/min under enter
Row strain fermentation 50-80h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitre
It is 5-7 to change bacterium, actinomyces, the weight ratio of photosynthetic bacteria:3-4:2-4:2-3:1-3:1-2；
(E) step (D) completed into the zymotic fluid concentration of strain fermentation, dried to water content≤3%, it is size-reduced to cross 100-300 mesh
Sieve, is obtained Streptococcus iniae disease biological agent.
3. a kind of application of the disease of Streptococcus iniae according to claim 1 biological agent, it is characterised in that the dolphin hammer
In tilapia feed, the tilapia feed is in units of weight portion, including following raw material for bacterium diease occurrence thing formulation application：Albumen
Powder 30-50 parts, Soybean Meal 10-18 parts, peanut meal 6-12 parts, corn 8-14 parts, Cottonseed Meal 4-7 parts, rapeseed dregs 5-8 parts, muscle high
Flour 10-16 parts, vitamin 1.8-3 parts, -2 parts of amino acid/11,1-1.8 parts of trace element, vegetable oil 5-8 parts, phagostimulant 1-2
Part, Streptococcus iniae 0.6-0.9 parts of biological agent of disease.
4. the application of Streptococcus iniae disease biological agent according to claim 3, it is characterised in that the vitamin is with weight
Part is unit, composed of the following components：Raw 0.8-1.2 parts of element A, vitamin B10.6-1 parts, vitamin C 0.4-0.8 parts.
5. the application of Streptococcus iniae disease biological agent according to claim 3, it is characterised in that the amino acid is with weight
Part is unit, composed of the following components：Phenylalanine 0.5-0.8 parts, glutamic acid 0.3-0.7 parts, serine 0.2-0.5 parts.
6. the application of Streptococcus iniae disease biological agent according to claim 3, it is characterised in that the trace element is with weight
Amount part is unit, composed of the following components：Copper 0.05-0.1 parts, manganese element 0.05-0.1 parts, chromium 0.05-0.1 parts,
Ferro element 0.2-0.45 parts, Zn-ef ficiency 0.2-0.45 parts, selenium element 0.25-0.3 parts, I 0.2-0.3 parts.
7. the application of Streptococcus iniae disease biological agent according to claim 3, it is characterised in that the vegetable oil includes flower
One or more in oil generation, soybean oil, linseed oil, castor oil, rape seed oil.
8. the application of Streptococcus iniae disease biological agent according to claim 3, it is characterised in that the phagostimulant, with weight
Amount part is unit, including following raw material：Anise 6-18 parts, matrimony vine 5-15 parts, Poria cocos 4-12 parts, peppermint 3-10 parts, hawthorn 4-8
Part, purslane 2-6 parts, garlic 4-6 parts, Chinese cassia tree 2-4 parts, dimethyl-β-DMPT 20-30 parts, glycine betaine 15-23 parts, core
Thuja acid 5-10 parts, sweetener 0.6-1 parts, flavouring agent 0.2-0.5 parts, acid 1-3 parts, 0.4-0.8 parts of pungent agent, tasty agents
0.3-0.6 parts；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
9. the application of Streptococcus iniae disease biological agent according to claim 8, it is characterised in that the preparation of the phagostimulant
Method, comprises the following steps：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to≤8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree to be crushed
Mixing, adds 150-300 parts of water, is 1 in solid-liquid ratio:12-14 (W/V), microwave power is 170-200W, and Extracting temperature is 89-
94 DEG C, extraction time is 1.2-1.5h, then filter residue, concentration, dry to water content≤3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, fragrance in medicinal powder obtained in step (b)
Agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained.
10. the application of Streptococcus iniae disease biological agent according to claim 3, it is characterised in that the tilapia feed
Preparation method, comprise the following steps：
S1：Soybean Meal, peanut meal, corn, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 100-300 mesh
Close uniform, homogeneous mixture is obtained；
S2：To in homogeneous mixture obtained in step S1 add albumen powder, Strong flour, vitamin, amino acid, trace element,
Vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 100-160 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, the microorganism is raised
Material is in units of weight portion, including following raw material：Agricultural waste material 40-48 parts, vegetables slag 30-40 parts, water 45-52 parts, bacterium
Plant 8-16 parts, cavings 12-22 parts of leavening；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：By the pure worm 326-352g of step (2), by material ratio 1:3.5-3.8 (W/V) adds water grinding, in 76-
Sterilize 24-26min at 78 DEG C, is cooled to 34-40 DEG C, and slurries are obtained；
(4) slurry hydrolysis：To cysteine proteinase 0.26-0.3g is added in slurries obtained in step (3), adjusting pH value is
5.6-6, hydrolyzes 37-41h at 46-50 DEG C, and then sterilize 25-30min at 70-73 DEG C, centrifugal filtration, and hydrolyzate is obtained；
(5) hydrolyzate ultrafiltration：Hydrolyzate obtained in step (4) is used into the hollow-fibre membrane that molecular cut off is 7000-8000
Ultrafiltration, is obtained ultrafiltrate；
(6) dry, crush, sieve：Ultrafiltrate obtained in step (5) is carried out into freeze-drying, 200-300 mesh sieves is crossed after crushing,
Albumen powder is obtained；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1-0.3cm and temperature be 90-105 DEG C at dry to
Moisture≤3.6%, is finally cooled to 25-30 DEG C, and tilapia feed is obtained.
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| CN109699849A (en) * | 2018-11-21 | 2019-05-03 | 广西南宁市武鸣明山红农业科技开发有限公司 | Improve the biological agent preparation process that Tilapia mossambica suffers from Streptococcus iniae disease cure rate |
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