CN101971928A - Artificial compound feed with function of preventing tilapia mossambica streptococcicosis - Google Patents
Artificial compound feed with function of preventing tilapia mossambica streptococcicosis Download PDFInfo
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- CN101971928A CN101971928A CN2010105154012A CN201010515401A CN101971928A CN 101971928 A CN101971928 A CN 101971928A CN 2010105154012 A CN2010105154012 A CN 2010105154012A CN 201010515401 A CN201010515401 A CN 201010515401A CN 101971928 A CN101971928 A CN 101971928A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention relates to a tilapia mossambica feed, in particular to an artificial compound feed for tilapia mossambica. An artificial compound feed with function of preventing tilapia mossambica streptococcicosis comprises the following components in percentage by mass: 5 to 10 percent of fish meal, 20 to 30 percent of bean pulp, 20 to 30 percent of rape pulp, 10 to 15 percent of cotton pulp, 10 to 15 percent of DDGS (maize alcohol lees and residual liquid dry substance), 5 to 10 percent of rice bran, 15 to 20 percent of flour, 5 to 10 percent of maize, 1 to 2 percent of soybean oil, 0.5 to 1.5 percent of phospholipid oil, 1 to 2 percent of calcium hydrogen phosphate, 1 to 10 percent of bentonite, 0.5 to 1 percent of multi-dimensional multi-mineral premix, 0.1 to 0.5 percent of choline chloride, 0.01 to 0.05 percent of antioxidant, 0.1 to 0.5 percent of mildew-proof agent and 1 to 5 percent of composite Chinese medicinal additive. Multiple antibacterial Chinese medicinal herb additives are added into the feed, so the feed improves the antibacterial capability of the tilapia mossambica and can prevent the tilapia mossambica streptococcicosis.
Description
[technical field]
The present invention relates to a kind of tilapia feed, relate in particular to a kind of Tilapia mossambica artifical compound feed.
[background technology]
Tilapia mossambica reaches more than 1,200,000 tons in the breed amount of China every year, accounts for 60% of the world.Tilapia mossambica growth rapidly, aquaculture cost is low, premunition is strong, the germ of many harm four large Chinese carps does not all influence Tilapia mossambica.But the Tilapia mossambica streptococcosis is in case outburst will cause unusual serious harm.Annual megathermal period streptococcus great outburst usually causes the Tilapia mossambica full pool death of breed.The Tilapia mossambica streptococcosis is outburst in a single day, and infectiousness is strong, longer duration, death rate height, and the high more state of an illness of water temperature is heavy more.The main pathogen of Tilapia mossambica streptococcosis comprises Streptococcus iniae, Streptococcusagalactiae, secondary streptococcus lactis, streptococcus dysgalactiae, sea dog streptococcus, form streptococcus, fish streptococcus lactis and Sha Shi streptococcus.The Tilapia mossambica streptococcosis is usually become the incurable disease of Tilapia mossambica by the raiser, just yield to no remedy in case mean outburst.Main cause is that streptococcosis infects brain and destroys nervous system, damages organs such as liver, kidney simultaneously again.Therefore for the Tilapia mossambica streptococcosis, should prevent greater than controlling.According to research reports, the reason of Tilapia mossambica streptococcosis morbidity mainly is to cause, and especially when feed nutrition was uneven, nitrogen discharged amount height caused water body ammonia nitrogen height, and dissolved oxygen is low.
Therefore, be necessary to provide a kind of prevention Tilapia Streptococcus pellet, when providing feed balanced in nutrition, prevent the outburst of Tilapia mossambica streptococcosis for the Tilapia mossambica growth.
[summary of the invention]
The purpose of this invention is to provide and a kind ofly can use, have prevention Tilapia mossambica streptococcosis function and artifical compound feed balanced in nutrition culturing high temperature season.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of artifical compound feed with prevention Tilapia mossambica streptococcosis function, calculate by percentage to the quality, comprise fish meal 1-5%, dregs of beans 20-30%, dish dregs of rice 20-30%, cotton dregs 10-15%, DDGS (the dry thing of maize alcohol lees and raffinate): 10-15%, rice bran 5-10%, flour 15-20%, corn 5-10%, soya-bean oil 1-2%, phosphatide oil 0.5-1.5%, calcium monohydrogen phosphate 1-2%, bentonite 1-10%, many ore deposits of multidimensional pre-composition 0.5-1%, Choline Chloride 0.1-0.5%, antioxidant 0.01-0.05%, mould inhibitor 0.1-0.5%, compound Chinese medicine additive 1-5%.
Preferably, calculate by percentage to the quality, comprise fish meal 2-5%, dregs of beans 20-30%, dish dregs of rice 20-30%, cotton dregs 10-15%, DDGS (the dry thing of maize alcohol lees and raffinate): 10-15%, rice bran 5-10%, flour 15-20%, corn 5-10%, soya-bean oil 1-2%, phosphatide oil 0.5-1.5%, calcium monohydrogen phosphate 1-2%, bentonite 1-10%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.01-0.05%, mould inhibitor 0.1-0.5%, compound Chinese medicine additive 1-3%.
Albumen, energy, vitamin and mineral raw material adopt prior art in the feed, wherein, described compound Chinese medicine additive is with after mixing in Radix Codonopsis 10-20 part, Radix Isatidis 5-15 part, Herba Andrographitis 5-15 part, honeysuckle 5-15 part, rhizoma menispermi 5-15 part, coptis 5-15 part, golden cypress 5-15 part, rheum officinale 5-15 part, Radix Astragali 5-15 part of weight portion, pulverize with micronizer, smashing fineness reaches 100 orders.
With mixing in proportion after the above-mentioned raw materials pulverizing,, make tilapia feed after the extruding cutting by conventional feed machinery.
Compare with common tilapia feed, the present invention has following advantage: 1, the nitrogen of feed can be than balance, and Tilapia mossambica is to its digestibility height, can not cause in the water ammonia nitrogen too high and bring out the streptococcus outburst; 2, add multiple antibacterial Chinese herbal medicines additive in the feed, improved the antibacterial ability of Tilapia mossambica, can effectively prevent the generation of Tilapia mossambica streptococcosis.
[specific embodiment]
The invention will be further described below in conjunction with specific embodiment.
Embodiment 1
According to the mass percent meter, take off the feedstuff of stating after the pulverizing: fish meal 5%, dregs of beans 22%, the dish dregs of rice 20%, cotton dregs 10%, DDGS (the dry thing of maize alcohol lees and raffinate) 10%, rice bran 5%, flour 15%, corn 6%, soya-bean oil 1.2%, phosphatide oil 0.5%, calcium monohydrogen phosphate 1%, bentonite 1.79%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.01%, mould inhibitor 0.1%, compound Chinese medicine additive 1.5%.The compound Chinese medicine additive is after 15 parts of mixing of 15 parts of 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 15 parts of Radix Codonopsis, Radix Isatidis, Herba Andrographitis, honeysuckles, rhizoma menispermis, the coptiss, golden cypresses, rheum officinales, the Radix Astragali in weight portion, form with the micronizer pulverizing, smashing fineness reaches 100 orders.After raw material fully mixes,, make tilapia feed after the extruding cutting by feed manufacturing machine.
Present embodiment forage protein content is: 30%, and fat content is: 3.8%.
Present embodiment adopts attacks malicious the checking, and three groups are established in experiment, and 5 every group parallel.Three feeds utilized common Tilapia mossambica commercial feed a, common Tilapia mossambica commercial feed b, embodiment of the invention feeds of being respectively of group.Experiment is carried out in aquarium.The experiment beginning, it is approaching to put into size specification in each aquarium, and each 30 tail of the Tilapia mossambica of physical health, every urosome weigh about 6 grams.The total body weight difference of Tilapia mossambica restrains less than 1 in each aquarium.Experiment divides three phases to carry out.Stage one: 1 month by a definite date throw something and feed the stage, 9 of every mornings and afternoon 3 feeds Tilapia mossambica of feeding with correspondence, adopting is satiated with food throws something and feeds the feeding volume unanimity of each aquarium.Stage two: injection stage is 8 * 108 Streptococcus iniae bacterium liquid (take out purifying in the Tilapia mossambica body of bacterium liquid bacterial strain uses therefor by the death of trouble streptococcosis and cultivate acquisition) 1ml to every tail Tilapia mossambica injection concentration.Stage three, one month by a definite date observation stage, the Tilapia mossambica that continues to throw something and feed each experimental group by described method of stage one, observed and recorded Tilapia mossambica death condition simultaneously.Final experimental result statistics is as follows:
Experimental results show that the medicinal herb components that contains in the feed provided by the invention has antibacterial action, can effectively prevent the generation of Tilapia mossambica streptococcosis, reduce the injury of streptococcosis Tilapia mossambica.
The prescription of testing used Tilapia mossambica commercial feed a is: calculate by percentage to the quality and comprise fish meal 2%, dregs of beans 35%, the dish dregs of rice 35%, rice bran 10%, flour 14%, soya-bean oil 1%,, calcium monohydrogen phosphate 1%, bentonite 1%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.1%.
The prescription of Tilapia mossambica commercial feed b: calculate by percentage to the quality, comprise fish meal 2%, dregs of beans 30%, the dish dregs of rice 40%, rice bran 14%, flour 10%, soya-bean oil 1%,, calcium monohydrogen phosphate 1%, bentonite 1%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.1%.
Embodiment 2
According to the mass percent meter, take off the feedstuff of stating after the pulverizing: fish meal 2%, dregs of beans 20%, the dish dregs of rice 25%, cotton dregs 10%, DDGS (the dry thing of maize alcohol lees and raffinate) 10%, rice bran 5%, flour 15%, corn 5%, soya-bean oil 1.5%, phosphatide oil 0.5%, calcium monohydrogen phosphate 1%, bentonite 2.99%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.01%, mould inhibitor 0.1%, compound Chinese medicine additive 1%.The compound Chinese medicine additive is after 10 parts of mixing of 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 15 parts of Radix Codonopsis, Radix Isatidis, Herba Andrographitis, honeysuckles, rhizoma menispermis, the coptiss, golden cypresses, rheum officinales, the Radix Astragali in weight portion, form with the micronizer pulverizing, smashing fineness reaches 100 orders.After raw material fully mixes,, make tilapia feed after the extruding cutting by feed manufacturing machine.
Present embodiment forage protein content is: 30%, and fat content is: 3.8%.
Throw something and feed respectively in experiment net cage Tilapia mossambica (initial weight average 150g) two months of the feed that present embodiment is obtained and common Tilapia mossambica commercial feed is got the experiment fish afterwards, takes a blood sample with tail fin, makes Tilapia mossambica serum.The lysozyme content that detects in the Tilapia mossambica serum is a substrate with micrococcus lysodeikticus freeze-dried powder (Sigma company product), follows these steps to carry out: join substrate suspension (OD570nm=013～015) with the phosphate buffer of 1/15mol/L pH 6.9.Get the 3mL suspension in vitro, put in the ice bath, add 50 μ L serum mixings, survey its light absorption value A at the 570nm place
0Then test tube is moved into 30min in 25 ℃ of water-baths, place ice bath 10min after the taking-up immediately, survey the light absorption value A at its 570nm place with cessation reaction.By formula calculate activity of lysozyme: U=(A
0-A)/A
0, experimental result is as follows:
| Experimental group | The common commercial tilapia feed | Inventive embodiments card feed |
| Experiment sample amount (tail) | 80 | 80 |
| Antalzyme activity | 0.624±0.015 | 0.723±0.124 |
Experimental results show that feed provided by the invention can effectively improve the Tilapia mossambica non-specific immunity, thereby play having an effect of prevention streptococcosis.
The prescription of testing used common commercial tilapia feed is: calculate by percentage to the quality, comprise fish meal 2%, dregs of beans 35%, the dish dregs of rice 35%, rice bran 10%, flour 14%, soya-bean oil 1%,, calcium monohydrogen phosphate 1%, bentonite 1%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.1%.
Embodiment 3
According to the mass percent meter, take off the feedstuff of stating after the pulverizing: fish meal 3%, dregs of beans 23%, the dish dregs of rice 20%, cotton dregs 10%, DDGS (the dry thing of maize alcohol lees and raffinate) 10%, rice bran 5%, flour 15%, corn 6%, soya-bean oil 1.4%, phosphatide oil 0.5%, calcium monohydrogen phosphate 1%, bentonite 2.89%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.01%, mould inhibitor 0.1%, compound Chinese medicine additive 1.2%.The compound Chinese medicine additive is 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 10 parts of 20 parts of Radix Codonopsis, Radix Isatidis, Herba Andrographitis, honeysuckles, rhizoma menispermis, the coptiss, golden cypresses, rheum officinales, 10 parts of the Radix Astragali in weight portion, form with the micronizer pulverizing, smashing fineness reaches 100 orders.After raw material fully mixes,, make tilapia feed after the extruding cutting by feed manufacturing machine.
Present embodiment forage protein content is: 30%, and fat content is: 3.8%, the medicinal herb components that contains in the feed has antibacterial action, can effectively prevent the generation of Tilapia mossambica streptococcosis.
Throw something and feed respectively in experiment net cage Tilapia mossambica (initial weight average 100g) two months of the feed that present embodiment is obtained and common Tilapia mossambica commercial feed is got the experiment fish afterwards, takes a blood sample with tail fin, makes Tilapia mossambica serum.The lysozyme content that detects in the Tilapia mossambica serum is a substrate with micrococcus lysodeikticus freeze-dried powder (Sigma company product), follows these steps to carry out: join substrate suspension (OD570nm=013～015) with the phosphate buffer of 1/15mol/L pH 6.9.Get the 3mL suspension in vitro, put in the ice bath, add 50 μ L serum mixings, survey its light absorption value A at the 570nm place
0Then test tube is moved into 30min in 25 ℃ of water-baths, place ice bath 10min after the taking-up immediately, survey the light absorption value A at its 570nm place with cessation reaction.By formula calculate activity of lysozyme: U=(A
0-A)/A
0, experimental result is as follows:
| Experimental group | The common commercial tilapia feed | Inventive embodiments card feed |
| Experiment sample amount (tail) | 120 | 120 |
| Antalzyme activity | 0.664±0.035 | 0.772±0.154 |
The prescription of testing used common commercial tilapia feed is: calculate by percentage to the quality, comprise fish meal 2%, dregs of beans 35%, the dish dregs of rice 35%, rice bran 10%, flour 14%, soya-bean oil 1%,, calcium monohydrogen phosphate 1%, bentonite 1%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.1%.
Experimental results show that feed provided by the invention can effectively improve the Tilapia mossambica non-specific immunity, thereby play having an effect of prevention streptococcosis.
Claims (3)
1. one kind has the artifical compound feed that prevents Tilapia mossambica streptococcosis function, it is characterized in that calculating by percentage to the quality, comprise fish meal 1-5%, dregs of beans 20-30%, dish dregs of rice 20-30%, cotton dregs 10-15%, DDGS (the dry thing of maize alcohol lees and raffinate): 10-15%, rice bran 5-10%, flour 15-20%, corn 5-10%, soya-bean oil 1-2%, phosphatide oil 0.5-1.5%, calcium monohydrogen phosphate 1-2%, bentonite 1-10%, many ore deposits of multidimensional pre-composition 0.5-1%, Choline Chloride 0.1-0.5%, antioxidant 0.01-0.05%, mould inhibitor 0.1-0.5%, compound Chinese medicine additive 1-5%.
2. the artifical compound feed with prevention Tilapia mossambica streptococcosis function as claimed in claim 1, it is characterized in that calculating by percentage to the quality, comprise fish meal 2-5%, dregs of beans 20-30%, dish dregs of rice 20-30%, cotton dregs 10-15%, DDGS (the dry thing of maize alcohol lees and raffinate): 10-15%, rice bran 5-10%, flour 15-20%, corn 5-10%, soya-bean oil 1-2%, phosphatide oil 0.5-1.5%, calcium monohydrogen phosphate 1-2%, bentonite 1-10%, many ore deposits of multidimensional pre-composition 0.7%, Choline Chloride 0.2%, antioxidant 0.01-0.05%, mould inhibitor 0.1-0.5%, compound Chinese medicine additive 1-3%.
3. the artifical compound feed with prevention Tilapia mossambica streptococcosis function as claimed in claim 1, it is characterized in that described compound Chinese medicine additive is with after mixing in Radix Codonopsis 10-20 part, Radix Isatidis 5-15 part, Herba Andrographitis 5-15 part, honeysuckle 5-15 part, rhizoma menispermi 5-15 part, coptis 5-15 part, golden cypress 5-15 part, rheum officinale 5-15 part, Radix Astragali 5-15 part of weight portion, pulverize with micronizer, smashing fineness reaches 100 orders.
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