CN104472873A - Feed additive for improving liver function of tilapia and preventing streptococcicosis - Google Patents

Feed additive for improving liver function of tilapia and preventing streptococcicosis Download PDF

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Publication number
CN104472873A
CN104472873A CN201410660644.3A CN201410660644A CN104472873A CN 104472873 A CN104472873 A CN 104472873A CN 201410660644 A CN201410660644 A CN 201410660644A CN 104472873 A CN104472873 A CN 104472873A
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tilapia
vitamin
liver
feed addictive
streptococcosis
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CN201410660644.3A
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CN104472873B (en
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段元慧
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GUANGZHOU HINTER BIOTECHNOLOGY CO Ltd
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GUANGZHOU HINTER BIOTECHNOLOGY CO Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Abstract

The invention provides a feed additive for improving liver function of tilapia and preventing streptococcicosis. The feed additive is prepared from radix bupleuri, immature bitter orange, patrinia, salvia miltiorrhiza, rhizoma pinellinae praeparata, rhizoma alismatis, oriental wormwood, glossy privet fruit, liquorice, yeast cell wall extract, vitamin C, vitamin E, choline and pantothenic acid. The feed additive is added into the tilapia feed to remarkably improve the liver health condition of the tilapia, improvie the immunity and enhance the antistreptococcic ability, and besides, the antibiotics drugs are effectively reduced during feeding, and the side effect caused by the antibiotics is reduced.

Description

A kind of feed addictive improving liver of hybrid tilapia function, prevention streptococcosis
[technical field]
The present invention relates to a kind of feed addictive, be specifically related to one and liver of hybrid tilapia function can be promoted to improve and the pathogenetic feed addictive of prevention Tilapia mossambica streptococcus.
[background technology]
Along with Tilapia mossambica cultivation scale expands gradually, the occurrence and infection also showed increased of various communicable disease, especially streptococcosis, popular on a large scale in recent years and in the trend that increases year by year, infectiousness is strong, the death rate reaches 50-70%, and serious harm is to the sound development of Chinese Tilapia mossambica aquaculture.It is impaired that ill Tilapia mossambica generally there will be the liver degree of depth, the symptoms such as apparent appearance flower liver, pale, the green liver of color, enlargement, show the liver function serious loss of disease fish, premunition serious loss, and this damage is all irreversible, result also in streptococcosis once outburst, be difficult to find effective remedy measures, therefore, prevent overweighting controlling for streptococcosis, strengthen liver of hybrid tilapia function by daily health service, improve the sick ability of Tilapia mossambica streptococcus significant.
[summary of the invention]
The object of this invention is to provide one can improve liver of hybrid tilapia function, improve Tilapia mossambica immunity, to reach safe, the efficient feed addictive of prevention Tilapia mossambica streptococcosis function.
For achieving the above object, the technical solution used in the present invention is as follows:
Improve a feed addictive for liver of hybrid tilapia function, prevention streptococcosis, comprise radix bupleuri, the dried immature fruit of citron orange, field pennycress, the red sage root, rhizoma pinellinae praeparata, rhizoma alismatis, oriental wormwood, the fruit of glossy privet, Radix Glycyrrhizae, yeast cell wall extract, vitamin C, vitamin E, choline, pantothenic acid.
Preferably, calculate by percentage to the quality, said components content is respectively: radix bupleuri 6-7%, dried immature fruit of citron orange 6-7%, field pennycress 6-7%, red sage root 7.5-8.75%, rhizoma pinellinae praeparata 7.5-8.75%, rhizoma alismatis 7.5-8.75%, oriental wormwood 7.5-8.75%, fruit of glossy privet 3-3.5%, Radix Glycyrrhizae 3-3.5%, yeast cell wall extract 12.87-16.59%, vitamin C 0.93-2.29%, vitamin E 0.93-2.29%, choline 5.87-9.59%, pantothenic acid 0.6-2.66%, carrier 3.58-24.8%.
Preferred, calculate by percentage to the quality, said components content is respectively: radix bupleuri 6.5%, the dried immature fruit of citron orange 6.5%, field pennycress 6.5%, the red sage root 8.125%, rhizoma pinellinae praeparata 8.125%, rhizoma alismatis 8.125%, oriental wormwood 8.125%, the fruit of glossy privet 3.25%, Radix Glycyrrhizae 3.25%, yeast cell wall extract 14.73%, vitamin C 1.36%, vitamin E 1.36%, choline 8.73%, pantothenic acid 1.68%, carrier 13.64%.
Yeast cell wall extract selected by the present invention refers to saccharomyces cerevisiae to be raw material, through the class fungal extract that breaking-wall cell, enzymolysis, separating-purifying and drying process are refined, finished product is light grey powder, wherein β-yeast dextran content is 25-60%, and manna oligosacchride content is 30-60%.
As required, appropriate carrier can also be added in composite feed additive of the present invention.
The percentage that composite feed additive of the present invention adds in feed is 1-4%, is preferably 2%.
Composite feed additive of the present invention is obtained by following methods: yeast cell wall extract crosses 80 mesh sieves after pulverizing, Chinese medicinal material drying dry doubling weighs up rear ultramicro grinding and crosses 80 mesh sieves, above-mentioned crushed material is mixed, adds vitamin C successively by corresponding proportion respectively, vitamin E, choline, pantothenic acid mixes.
This real beneficial effect of the invention is:
(1) constituent of the present invention is based on Chinese herbal medicine, vitamin, probio, and not containing any poisonous and harmful element, safe noresidue, security is high.
(2) proportioning between recipe ingredient of the present invention is even, obviously can improve the liver health situation of Tilapia mossambica, improves immunity, strengthens the ability of streptococcus disease.Effectively can reduce the use of antibiotics in the process of throwing something and feeding simultaneously, reduce the side effect that antibiotic causes.
(3) the raw materials used steady sources of the present invention, be easy to get, production technology is simple, be easy to industrialization and produce.
[detailed description of the invention]
Explain the present invention further below in conjunction with embodiment, but embodiment does not limit in any form to the present invention.
Embodiment 1
A kind of composite feed additive improving liver of hybrid tilapia function, prevention streptococcosis, its formula is: radix bupleuri 6.5%, the dried immature fruit of citron orange 6.5%, field pennycress 6.5%, the red sage root 8.125%, rhizoma pinellinae praeparata 8.125%, rhizoma alismatis 8.125%, oriental wormwood 8.125%, the fruit of glossy privet 3.25%, Radix Glycyrrhizae 3.25%, yeast cell wall extract 14.73%, vitamin C 1.36%, vitamin E 1.36%, choline 8.73%, pantothenic acid 1.68%, carrier 13.64%.β in wherein said yeast cell wall extract-yeast dextran content is 39%, and manna oligosacchride content is 45%, and described carrier is time powder.
This additive obtains by the following method: yeast cell wall extract pulverized 80 mesh sieves, Chinese medicinal material drying dry doubling weighs up rear ultramicro grinding and crosses 80 mesh sieves, mixed with carrier by above-mentioned crushed material, vitamin C, vitamin E, choline, pantothenic acid add in mixture the composite feed additive mixing and make containing carrier respectively successively to by corresponding proportion.
Embodiment 2
A kind of composite feed additive improving liver of hybrid tilapia function, prevention streptococcosis, its formula is: radix bupleuri 7%, the dried immature fruit of citron orange 7%, field pennycress 7%, the red sage root 8.75%, rhizoma pinellinae praeparata 8.75%, rhizoma alismatis 8.75%, oriental wormwood 8.75%, the fruit of glossy privet 3.5%, Radix Glycyrrhizae 3.5%, yeast cell wall extract 16.59%, vitamin C 2.29%, vitamin E 2.29%, choline 9.59%, pantothenic acid 2.66%, carrier 3.58%.β in wherein said yeast cell wall extract-yeast dextran content is 60%, and manna oligosacchride content is 60%, and described carrier is time powder.
Composite feed additive of the present invention is obtained according to the preparation method of embodiment 1.
Embodiment 3
A kind of composite feed additive improving liver of hybrid tilapia function, prevention streptococcosis, its formula is: radix bupleuri 6%, the dried immature fruit of citron orange 6%, field pennycress 6%, the red sage root 7.5%, rhizoma pinellinae praeparata 7.5%, rhizoma alismatis 7.5%, oriental wormwood 7.5%, the fruit of glossy privet 3%, Radix Glycyrrhizae 3%, yeast cell wall extract 12.87%, vitamin C 0.93%, vitamin E 0.93%, choline 5.87%, pantothenic acid 0.6%, carrier 24.8%.β in wherein said yeast cell wall extract-yeast dextran content is 25%, and manna oligosacchride content is 30%, and described carrier is time powder.
Composite feed additive of the present invention is prepared according to the method for embodiment 1.
Below the compliance test result experiment that composite feed additive of the present invention improves liver of hybrid tilapia function, prevention streptococcosis:
Composite feed additive obtained for embodiment 1 is added in tilapia feed in the ratio of 1%, 2%, 3%, 4% respectively, be respectively experimental group 1, experimental group 2, test group 3 and experimental group 4, and set up control group (not containing composite feed additive), each processed group 3 is parallel.Experiment is carried out in net cage, and cage size is 2m × 2m × 2m.Experiment start, it is close that size specification put into by each net cage, each 100 tails of Tilapia mossambica of physical health, and every urosome is heavily about 100g, in each net cage Tilapia mossambica must weight differences less than 20g.An experiment point three phases carries out, the stage one: 15 days by a definite date throw something and feed the stage, every morning 9:00 and afternoon 3:00 feed Tilapia mossambica with corresponding feed respectively, adopting is satiated with food throws something and feeds, and the feeding volume of each net cage is consistent; Stage two: the poison stage is attacked in injection, is 2 × 10 to every tail Tilapia mossambica muscle of back injection concentration 9cfu/mL Streptococcusagalactiae bacterium liquid (separation in Maoming, the sick fish body in cultivation market, Gaozhou county, purifying, qualification, cultivation obtain) 0.5ml; Stage three: one-week observation stage, the death condition of statistics Tilapia mossambica.
The rate statistics that each group of Tilapia mossambica survives is as shown in table 1, and result display adopts the feedstuff feeding Tilapia mossambica containing feed addictive of the present invention, after two weeks, can strengthen the antistreptococcic ability of Tilapia mossambica, significantly improve Tilapia mossambica attack poison after survival rate.
Table 1: the survival rate of each group Tilapia mossambica
Group Control group Experimental group 1 Experimental group 2 Experimental group 3 Experimental group 4
Survival rate (%) 75.4 80.5 92.3 81.2 85
At the end of stage one, from often organize get at random 5 tail Tilapia mossambicas blood drawing obtain serum, for measuring the activity of Tilapia mossambica serum glutamic oxalacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT).Get the liver homogenate of 2 tail Tilapia mossambicas in addition, measure superoxide dismutase (SOD) active.The activity of serum glutamic oxalacetic transaminase in serum (GOT) and glutamic-pyruvic transaminase (GPT) can be caused to increase when liver damage.In addition, when liver is subject to virus infraction, inflammatory cell infiltration in liver cell, leukocyte activation produces the free radicals such as a large amount of superoxide ion and peroxide, and the removing of free radical is mainly undertaken by some peroxidase (SOD), their vigor and content reduce all will cause the decline of fish liver oxidation resistance and hepatocellular injury.
Measurement result is as shown in table 2, composite feed additive of the present invention significantly can reduce serum GOT level and the GPT level of Tilapia mossambica, illustrate that this additive has the effect alleviating hepatic injury, in addition, it is active, wherein the highest with the SOD activity of experimental group 2 that this additive can also obviously increase liver SOD.Illustrate that composite feed additive of the present invention is by playing the effectively pathogenetic effect of prevention streptococcus to the strengthening of liver function.
Table 2: serum, the enzyme activity of liver of each group Tilapia mossambica
Group Serum GOT (U/L) Serum GPT (U/L) Liver SOD (U/g)
Control group 48±3.52 50±3.01 465±12.34
Experimental group 1 45±3.82 49±4.23 478±11.23
Experimental group 2 42±1.63 46±2.87 490±14.34
Experimental group 3 43±2.72 46±2.67 485±15.67
Experimental group 4 42±2.01 47±3.03 487±14.98

Claims (9)

1. one kind is improved the feed addictive of liver of hybrid tilapia function, prevention streptococcosis, it is characterized in that, described feed addictive comprises radix bupleuri, the dried immature fruit of citron orange, field pennycress, the red sage root, rhizoma pinellinae praeparata, rhizoma alismatis, oriental wormwood, the fruit of glossy privet, Radix Glycyrrhizae, yeast cell wall extract, vitamin C, vitamin E, choline, pantothenic acid.
2. the feed addictive improving liver of hybrid tilapia function, prevention streptococcosis according to claim 1, it is characterized in that, described feed addictive comprises radix bupleuri 6-7%, dried immature fruit of citron orange 6-7%, field pennycress 6-7%, red sage root 7.5-8.75%, rhizoma pinellinae praeparata 7.5-8.75%, rhizoma alismatis 7.5-8.75%, oriental wormwood 7.5-8.75%, fruit of glossy privet 3-3.5%, Radix Glycyrrhizae 3-3.5%, yeast cell wall extract 12.87-16.59%, vitamin C 0.93-2.29%, vitamin E 0.93-2.29%, choline 5.87-9.59%, pantothenic acid 0.6-2.66%, carrier 3.58-24.8%.
3. the feed addictive improving liver of hybrid tilapia function, prevention streptococcosis according to claim 2, it is characterized in that, described feed addictive is made up of following component: radix bupleuri 6.5%, the dried immature fruit of citron orange 6.5%, field pennycress 6.5%, the red sage root 8.125%, rhizoma pinellinae praeparata 8.125%, rhizoma alismatis 8.125%, oriental wormwood 8.125%, the fruit of glossy privet 3.25%, Radix Glycyrrhizae 3.25%, yeast cell wall extract 14.73%, vitamin C 1.36%, vitamin E 1.36%, choline 8.73%, pantothenic acid 1.68%, carrier 13.64%.
4. the feed addictive of the improvement liver of hybrid tilapia function according to any one of claim 1-3, prevention streptococcosis, it is characterized in that: in described yeast cell wall extract, β-yeast dextran content is 25-60%, manna oligosacchride content is 30-60%.
5. the feed addictive of the improvement liver of hybrid tilapia function according to Claims 2 or 3, prevention streptococcosis, is characterized in that: described carrier is time powder.
6. the preparation method of the feed addictive of the improvement liver of hybrid tilapia function according to any one of claim 1-4, prevention streptococcosis, it is characterized in that, yeast cell wall extract crosses 80 mesh sieves after pulverizing, Chinese medicinal material drying dry doubling weighs up rear ultramicro grinding and crosses 80 mesh sieves, above-mentioned crushed material is mixed, adds vitamin C successively by corresponding proportion respectively, vitamin E, choline, pantothenic acid mixes.
7. a tilapia feed, is characterized in that, comprises the feed addictive of the improvement liver of hybrid tilapia function according to any one of claim 1-4, prevention streptococcosis.
8. tilapia feed according to claim 7, is characterized in that, describedly improves liver of hybrid tilapia function, the adding proportion of feed addictive of prevention streptococcosis is 1-4%.
9. tilapia feed according to claim 8, is characterized in that, describedly improves liver of hybrid tilapia function, the adding proportion of feed addictive of prevention streptococcosis is 2%.
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CN105231100A (en) * 2015-11-16 2016-01-13 江苏华威农牧发展有限公司 Feed capable of preventing liver and gallbladder syndromes of crucian carps under high-density raising conditions
CN105661096A (en) * 2015-12-30 2016-06-15 苏州市相城区太平蟹业专业合作社 Fish feed growth additive
CN106173561A (en) * 2016-08-19 2016-12-07 广西正五海洋产业股份有限公司 A kind of tilapia special feed
CN106551185A (en) * 2016-11-22 2017-04-05 防城港市海洋局 A kind of Chinese medicine feed additive for tilapia and preparation method thereof
CN106616067A (en) * 2017-02-24 2017-05-10 广东恒兴饲料实业股份有限公司 Growth promoter for adult hybrid tilapia as well as preparation method and use method of growth promoter
CN109105668A (en) * 2018-09-20 2019-01-01 浦江县酉泽水产科技有限公司 The preparation method of growth promotion tilapia feed
CN109105669A (en) * 2018-09-20 2019-01-01 浦江县酉泽水产科技有限公司 Improve the preparation method of Tilapia mossambica immunity feed addictive

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105231100A (en) * 2015-11-16 2016-01-13 江苏华威农牧发展有限公司 Feed capable of preventing liver and gallbladder syndromes of crucian carps under high-density raising conditions
CN105661096A (en) * 2015-12-30 2016-06-15 苏州市相城区太平蟹业专业合作社 Fish feed growth additive
CN106173561A (en) * 2016-08-19 2016-12-07 广西正五海洋产业股份有限公司 A kind of tilapia special feed
CN106551185A (en) * 2016-11-22 2017-04-05 防城港市海洋局 A kind of Chinese medicine feed additive for tilapia and preparation method thereof
CN106616067A (en) * 2017-02-24 2017-05-10 广东恒兴饲料实业股份有限公司 Growth promoter for adult hybrid tilapia as well as preparation method and use method of growth promoter
CN109105668A (en) * 2018-09-20 2019-01-01 浦江县酉泽水产科技有限公司 The preparation method of growth promotion tilapia feed
CN109105669A (en) * 2018-09-20 2019-01-01 浦江县酉泽水产科技有限公司 Improve the preparation method of Tilapia mossambica immunity feed addictive

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