CN106721534A - A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease and preparation method thereof - Google Patents
A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease and preparation method thereof Download PDFInfo
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- CN106721534A CN106721534A CN201611081610.4A CN201611081610A CN106721534A CN 106721534 A CN106721534 A CN 106721534A CN 201611081610 A CN201611081610 A CN 201611081610A CN 106721534 A CN106721534 A CN 106721534A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease and preparation method thereof, the feed is in units of weight portion, including following raw material：30 50 parts of albumen powder, 35 parts of beet pulp, 46 parts of earthworm powder, 12 18 parts of Ipomoea batatas, 47 parts of Cottonseed Meal, 58 parts of rapeseed dregs, 9 14 parts of flour, 1.8 3 parts of vitamin, 2 parts of amino acid/11,1 1.8 parts of trace element, 58 parts of vegetable oil, 12 parts of phagostimulant, Streptococcus iniae 0.6 0.9 parts of biological agent of disease, the feed are made by the step such as drying, crushing, mix, dry.Feed formulation of the invention is reasonable, nutritious, can increase and suffer from Streptococcus iniae disease Tilapia mossambica feed intake, improves the speed of growth, strengthens body's immunity, reduces Tilapia mossambica and suffers from the sick death rate of Streptococcus iniae and improve cure rate.
Description
【Technical field】
The invention belongs to tilapia feed preparing technical field, and in particular to a kind of preventing and treating Tilapia mossambica Streptococcus iniae disease
Feed and preparation method thereof.
【Background technology】
In the production process of biological feedstuff, addition is common insect albumen powder, the production work of common insect albumen powder
It is that worm's ovum is cultivated as culture with wheat bran, green vegetables in skill, after worm's ovum is grown up to pure worm, to pure worm fried dry or microwave
Baking oven is done, and is then comminuted into worm powder, then worm powder is mixed with culture or fermented bean dregs or general proteins raw material, forms common
Insect albumen powder, in the production technology, because the culture cultivated worm's ovum is wheat bran, green vegetables, therefore the pure worm for growing up to
Composition in be free of antibacterial peptide and saccharomycete, therefore effect without enhance immunity；Due to using the drying to pure worm fried dry
Mode, easily makes protein denaturation, so as to destroy due nutritional ingredient；Due to crushing to form worm powder pure worm, do not make water
Solution treatment, composition exists in the form of the protein of intrinsic macromolecular or in the form of polypeptide, therefore is not easy to be absorbed.
Raising of the phagostimulant to Tilapia mossambica has obvious and positive biological action, can improve the attractant of feed and fit
Mouth property, improves food conversion ratio, and then improves the production performance of Tilapia mossambica comprehensively, such as increases feed intake, raising daily gain, improves
Meat quality etc..One side phagostimulant can cover the bad smell in feed by special odor, stimulate the sensation of Tilapia mossambica
Organ, to reach the purpose for promoting Tilapia mossambica to ingest, accelerate Growth Op Tilapia；On the other hand, Tilapia mossambica is induced by phagostimulant
Ingest, shorten the time of ingesting, improve the utilization rate of feed.It is flavouring agent or sweet taste that phagostimulant in the market is most of
Agent, effect is more single, can only form single stimulation to the smell of Tilapia mossambica or vision or the sense of taste etc.；And some flavouring agents or
The constituent of sweetener comes from chemically synthesized product, produces medicament residue, toxic and side effect obvious easily in Tilapia mossambica body.Cause
This, how by these single Attractants be compounded to form effect substantially, steady quality and economical and practical phagostimulant is trend
Place.
Streptococcus is widely present in nature, people and animal, causes various purulent inflammations, scarlet fever, erysipelas, neonate
Septicemia, meningitis, puerperal fever and streptococcus allergic disease etc..Aquiculture animal can also infect streptococcus generation
Streptococcosis, causes massive mortality, causes great economic loss to World Aquaculture every year.China is big aquaculture
State, every year because streptococcosis is to about 1,000,000,000 dollars of the direct economic loss caused by aquaculture.Tilapia mossambica be the important torrid zone and
Subtropical zone aquiculture animal, is also one of main object of streptococcal infection.2001 start, and Chinese Rofe fish culture is successively
The infection of streptococcosis is occurred in that, and the high mortality of 30%-50% is caused in indivedual plants.2009-2013 continuous 5
Year, streptococcosis in Chinese Rofe fish culture large area outbreak of epidemic, diseased region expands year by year, morbidity and mortality year by year
It is incremented by, susceptible Tilapia mossambica specification limit expands year by year.By the average annual Production rate of China Tilapia mossambica, estimate annual streptococcosis to me
Just up to 400,000,000 dollars of the direct economic loss that state's Tilapia mossambica industry is caused.Breaking out for disease is asked with the product safety brought therewith
Topic has turned into the bottleneck of restriction Tilapia mossambica industry development.Lack a set of effective scheme at present and prevent and treat the disease, medicine
Thing can only play the complementary effect of symptom management in early stage, but with resistance and drug-fast generation and accumulation, and the disease shows
Shape is almost " pasting medical help to prevent ".
With the increase of market demand, the outburst of various diseases causes tremendous influence to Tilapia mossambica industry, therefore
Effective prevention and treatment of Tilapia mossambica disease, particularly streptococcosis how are effectively prevented and treated, yield is improved, industry size is increased,
Development Tilapia mossambica healthy aquaculture technology has had very big necessity and urgency.
【The content of the invention】
The technical problem to be solved in the present invention is to provide a kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease and its preparation
Method, is difficult to prevent and treat to solve Tilapia mossambica streptococcosis, tilapia mossambica food with feed intake after existing feed it is low, the speed of growth is slow asks
Topic.Feed formulation of the invention is reasonable, nutritious, can increase and suffer from Streptococcus iniae disease Tilapia mossambica feed intake, improves growth speed
Degree, strengthens body's immunity, reduces Tilapia mossambica and suffers from the sick death rate of Streptococcus iniae and improve cure rate.
In order to solve the above technical problems, the present invention uses following technical scheme：
A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease, in units of weight portion, including following raw material：Albumen powder
30-50 parts, beet pulp 3-5 parts, earthworm powder 4-6 parts, Ipomoea batatas 12-18 parts, Cottonseed Meal 4-7 parts, rapeseed dregs 5-8 parts, flour 9-14
Part, vitamin 1.8-3 parts, -2 parts of amino acid/11,1-1.8 parts of trace element, vegetable oil 5-8 parts, phagostimulant 1-2 parts, dolphin hammer
Bacterium 0.6-0.9 parts of biological agent of disease.
Preferably, the vitamin is composed of the following components in units of weight portion：Raw 0.8-1.2 parts of element A, vitamin
B10.6-1 parts, vitamin C 0.4-0.8 parts.
Preferably, the amino acid is composed of the following components in units of weight portion：Phenylalanine 0.5-0.8 parts, paddy ammonia
It is sour 0.3-0.7 parts, serine 0.2-0.5 parts.
Preferably, the trace element is composed of the following components in units of weight portion：Copper 0.05-0.1 parts, manganese
Element 0.05-0.1 parts, chromium 0.05-0.1 parts, ferro element 0.2-0.45 parts, Zn-ef ficiency 0.2-0.45 parts, selenium element 0.25-
0.3 part, I 0.2-0.3 parts.
Preferably, the vegetable oil includes the one kind or many in peanut oil, soybean oil, linseed oil, castor oil, rape seed oil
Kind.
Preferably, the phagostimulant, in units of weight portion, including following raw material：Anise 6-18 parts, matrimony vine 5-15
Part, Poria cocos 4-12 parts, peppermint 3-10 parts, hawthorn 4-8 parts, purslane 2-6 parts, garlic 4-6 parts, Chinese cassia tree 2-4 parts, dimethyl-β-
DMPT 20-30 parts, glycine betaine 15-23 parts, nucleotides 5-10 parts, sweetener 0.6-1 parts, flavouring agent 0.2-0.5 parts, tart flavour
Agent 1-3 parts, 0.4-0.8 parts, tasty agents 0.3-0.6 parts of pungent agent；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The present invention also provides a kind of preparation method of the feed for preventing and treating Tilapia mossambica Streptococcus iniae disease, comprises the following steps：
S1：Beet pulp, earthworm powder, Ipomoea batatas, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, the sieve of 100-300 mesh is crossed
After be well mixed, be obtained homogeneous mixture；
S2：To in homogeneous mixture obtained in step S1 add albumen powder, flour, vitamin, amino acid, trace element,
Vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 100-160 parts of water, are well mixed again, and moist forage is obtained；
S3：Moist forage obtained in step S2 is made the particle of diameter 0.1-0.3cm and the baking in the case where temperature is for 90-105 DEG C
Do to moisture≤3.6%, be finally cooled to 25-30 DEG C, the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease is obtained.
Preferably, the preparation method of albumen powder described in step S2, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：Agricultural waste material 40-48 parts, vegetables slag 30-40 parts, water 45-52
Part, bacteria leaven 8-16 parts, cavings 12-22 parts；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：After by the pure worm high-temperature sterilization of step (2), slurries are milled into；
(4) slurry hydrolysis：To protease sealing hydrolysis is added in slurries obtained in step (3), hydrolysis time is 42-45h,
Temperature is 53-55 DEG C；
(5) concentrate, dry：It is dried and crushes after slurries concentration after being hydrolyzed to step (4), albumen powder is obtained.
Preferably, the preparation method of phagostimulant described in step S2, comprises the following steps：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to≤8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 150-300 parts of water, is 1 in solid-liquid ratio:12-14 (W/V), microwave power is 170-200W, and Extracting temperature is
89-94 DEG C, extraction time is 1.2-1.5h, then filter residue, concentration, dry to water content≤3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained.
Preferably, the preparation method of the disease of Streptococcus iniae described in step S2 biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：Peptone 180-220 parts, 130-150 parts of Yeast diffusion juice powder, beef extract 45-65 parts, 2-4 parts of trace element, water
1000-2000 parts, the trace element is in units of weight portion, including following raw material：Zn-ef ficiency 4-9 parts, selenium element 3-6 parts,
I 2-4 parts；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, actinomyces, temperature be 32-36 DEG C, rotating speed be 200-220r/min under carry out strain fermentation 50-
80h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces, light
The weight ratio for closing bacterium is 5-7:3-4:2-4:1-3；
(E) step (D) completed into the zymotic fluid concentration of strain fermentation, dried to water content≤3%, it is size-reduced to cross 100-
300 mesh sieve, are obtained Streptococcus iniae disease biological agent.
The invention has the advantages that：
(1) feed formulation of the invention is reasonable, nutritious, can increase and suffer from Streptococcus iniae disease Tilapia mossambica feed intake, carries
Seedling height speed；
(2) feed of the invention can play the effects such as anti-inflammation, enhancing body's immunity, can effectively reduce Rofe
The death rate and improve cure rate that fish suffers from Streptococcus iniae disease；
(3) feed synthesis low production cost of the invention, is conducive to popularization and application.
【Specific embodiment】
For ease of more fully understanding the present invention, it is illustrated by following examples, these embodiments belong to of the invention
Protection domain, but do not limit the scope of the invention.
In embodiment, the feed of the preventing and treating Tilapia mossambica Streptococcus iniae disease, in units of weight portion, including following original
Material：Albumen powder 30-50 parts, beet pulp 3-5 parts, earthworm powder 4-6 parts, Ipomoea batatas 12-18 parts, Cottonseed Meal 4-7 parts, rapeseed dregs 5-8 parts,
Flour 9-14 parts, vitamin 1.8-3 parts, -2 parts of amino acid/11,1-1.8 parts of trace element, vegetable oil 5-8 parts, phagostimulant 1-2 parts,
Streptococcus iniae 0.6-0.9 parts of biological agent of disease.
The vitamin is composed of the following components in units of weight portion：Raw 0.8-1.2 parts of element A, vitamin B1 0.6-
1 part, vitamin C 0.4-0.8 parts.
The amino acid is composed of the following components in units of weight portion：Phenylalanine 0.5-0.8 parts, glutamic acid 0.3-
0.7 part, serine 0.2-0.5 parts.
The trace element is composed of the following components in units of weight portion：Copper 0.05-0.1 parts, manganese element
0.05-0.1 parts, chromium 0.05-0.1 parts, ferro element 0.2-0.45 parts, Zn-ef ficiency 0.2-0.45 parts, selenium element 0.25-0.3
Part, I 0.2-0.3 parts.
The vegetable oil includes one or more in peanut oil, soybean oil, linseed oil, castor oil, rape seed oil.
The phagostimulant, in units of weight portion, including following raw material：Anise 6-18 parts, matrimony vine 5-15 parts, Poria cocos 4-
12 parts, peppermint 3-10 parts, hawthorn 4-8 parts, purslane 2-6 parts, garlic 4-6 parts, Chinese cassia tree 2-4 parts, dimethyl-β-DMPT
20-30 parts, glycine betaine 15-23 parts, nucleotides 5-10 parts, sweetener 0.6-1 parts, flavouring agent 0.2-0.5 parts, acid 1-3 parts,
0.4-0.8 parts, tasty agents 0.3-0.6 parts of pungent agent；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the feed of the preventing and treating Tilapia mossambica Streptococcus iniae disease, comprises the following steps：
S1：Beet pulp, earthworm powder, Ipomoea batatas, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, the sieve of 100-300 mesh is crossed
After be well mixed, be obtained homogeneous mixture；
S2：To in homogeneous mixture obtained in step S1 add albumen powder, flour, vitamin, amino acid, trace element,
Vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 100-160 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：Agricultural waste material 40-48 parts, vegetables slag 30-40 parts, water 45-52
Part, bacteria leaven 8-16 parts, cavings 12-22 parts；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：After by the pure worm high-temperature sterilization of step (2), slurries are milled into；
(4) slurry hydrolysis：To protease sealing hydrolysis is added in slurries obtained in step (3), hydrolysis time is 42-45h,
Temperature is 53-55 DEG C；
(5) concentrate, dry：It is dried and crushes after slurries concentration after being hydrolyzed to step (4), albumen powder is obtained；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to≤8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 150-300 parts of water, is 1 in solid-liquid ratio:12-14 (W/V), microwave power is 170-200W, and Extracting temperature is
89-94 DEG C, extraction time is 1.2-1.5h, then filter residue, concentration, dry to water content≤3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：Peptone 180-220 parts, 130-150 parts of Yeast diffusion juice powder, beef extract 45-65 parts, 2-4 parts of trace element, water
1000-2000 parts, the trace element is in units of weight portion, including following raw material：Zn-ef ficiency 4-9 parts, selenium element 3-6 parts,
I 2-4 parts；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, actinomyces, temperature be 32-36 DEG C, rotating speed be 200-220r/min under carry out strain fermentation 50-
80h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces, light
The weight ratio for closing bacterium is 5-7:3-4:2-4:1-3；
(E) step (D) completed into the zymotic fluid concentration of strain fermentation, dried to water content≤3%, it is size-reduced to cross 100-
300 mesh sieve, are obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 is made the particle of diameter 0.1-0.3cm and the baking in the case where temperature is for 90-105 DEG C
Do to moisture≤3.6%, be finally cooled to 25-30 DEG C, the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease is obtained.
It is illustrated below by more specifically embodiment.
Embodiment 1
A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease, in units of weight portion, including following raw material：Albumen powder 40
Part, 4 parts of beet pulp, 5 parts of earthworm powder, 16 parts of Ipomoea batatas, 6 parts of Cottonseed Meal, 7 parts of rapeseed dregs, 12 parts of flour, 2.5 parts of vitamin, amino
1.5 parts of acid, 1.4 parts of trace element, 7 parts of vegetable oil, 1.6 parts of phagostimulant, Streptococcus iniae 0.7 part of biological agent of disease.
The vitamin is composed of the following components in units of weight portion：Raw 1 part of element A, vitamin B10.8 part, dimension life
0.7 part of plain C.
The amino acid is composed of the following components in units of weight portion：0.7 part of phenylalanine, glutamic acid 0.5 part, thread
0.3 part of propylhomoserin.
The trace element is composed of the following components in units of weight portion：0.075 part of copper, manganese element 0.075
Part, 1 part of chromium, 0.3 part of ferro element, 0.3 part of Zn-ef ficiency, 0.3 part of selenium element, 0.25 part of I.
The vegetable oil is peanut oil.
The phagostimulant, in units of weight portion, including following raw material：It is 12 parts of anise, 10 parts of matrimony vine, 8 parts of Poria cocos, thin
7 parts of lotus, 6 parts of hawthorn, 4 parts of purslane, 10 parts of garlic, 3 parts of Chinese cassia tree, 25 parts of dimethyl-β-DMPT, 20 parts of glycine betaine, core
7 parts of thuja acid, 0.8 part of sweetener, 0.4 part of flavouring agent, 2 parts of acid, 0.6 part of pungent agent, 0.5 part of tasty agents；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the feed of the preventing and treating Tilapia mossambica Streptococcus iniae disease, comprises the following steps：
S1：Beet pulp, earthworm powder, Ipomoea batatas, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 200 mesh
Close uniform, homogeneous mixture is obtained；
S2：To in homogeneous mixture obtained in step S1 add albumen powder, flour, vitamin, amino acid, trace element,
Vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 140 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：45 parts of agricultural waste material, 35 parts of vegetables slag, 50 parts of water, strain fermentation
13 parts of agent, 18 parts of cavings；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：After by the pure worm high-temperature sterilization of step (2), slurries are milled into；
(4) slurry hydrolysis：To protease sealing hydrolysis is added in slurries obtained in step (3), hydrolysis time is 44h, temperature
Spend is 54 DEG C；
(5) concentrate, dry：It is dried and crushes after slurries concentration after being hydrolyzed to step (4), albumen powder is obtained；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to 8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 250 parts of water, is 1 in solid-liquid ratio:13 (W/V), microwave power is 185W, and Extracting temperature is 92 DEG C, during extraction
Between be 1.4h, then filter residue, concentration, to dry to water content be 3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：200 parts of peptone, 140 parts of Yeast diffusion juice powder, 55 parts of beef extract, micro- 3 parts, 1500 parts of water, it is described micro
Element is in units of weight portion, including following raw material：8 parts of Zn-ef ficiency, 5 parts of selenium element, 3 parts of I；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, actinomyces, temperature be 35 DEG C, rotating speed be 210r/min under carry out strain fermentation 65h, the red
Nocard's bacillus ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces, the weight ratio of photosynthetic bacteria
It is 6:3.5:3:2；
(E) it is 3%, the mesh of size-reduced mistake 200 to concentrate the zymotic fluid that step (D) completes strain fermentation, dry to water content
Sieve, is obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1-0.3cm and temperature be 100 DEG C at dry to
Moisture is 3.6%, is finally cooled to 28 DEG C, and the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease is obtained.
Embodiment 2
A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease, in units of weight portion, including following raw material：Albumen powder 30
Part, 3 parts of beet pulp, 4 parts of earthworm powder, 12 parts of Ipomoea batatas, 4 parts of Cottonseed Meal, 5 parts of rapeseed dregs, 9 parts of flour, 1.8 parts of vitamin, amino
1 part of acid, 1 part of trace element, 5 parts of vegetable oil, 1 part of phagostimulant, Streptococcus iniae 0.6 part of biological agent of disease.
The vitamin is composed of the following components in units of weight portion：Raw 0.8 part of element A, vitamin B10.6 part, dimension
Raw 0.4 part of element C.
The amino acid is composed of the following components in units of weight portion：0.5 part of phenylalanine, glutamic acid 0.3 part, thread
0.2 part of propylhomoserin.
The trace element is composed of the following components in units of weight portion：0.05 part of copper, 0.05 part of manganese element,
0.05 part of chromium, 0.2 part of ferro element, 0.2 part of Zn-ef ficiency, 0.25 part of selenium element, 0.2 part of I.
The vegetable oil is soybean oil.
The phagostimulant, in units of weight portion, including following raw material：6 parts of anise, 5 parts of matrimony vine, 4 parts of Poria cocos, peppermint
3 parts, 4 parts of hawthorn, 2 parts of purslane, 4 parts of garlic, 2 parts of Chinese cassia tree, 20 parts of dimethyl-β-DMPT, 15 parts of glycine betaine, nucleotides
5 parts, 0.6 part of sweetener, 0.2 part of flavouring agent, 1 part of acid, 0.4 part of pungent agent, 0.3 part of tasty agents；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the feed of the preventing and treating Tilapia mossambica Streptococcus iniae disease, comprises the following steps：
S1：Beet pulp, earthworm powder, Ipomoea batatas, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 100 mesh
Close uniform, homogeneous mixture is obtained；
S2：To in homogeneous mixture obtained in step S1 add albumen powder, flour, vitamin, amino acid, trace element,
Vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 100 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：40 parts of agricultural waste material, 30 parts of vegetables slag, 45 parts of water, strain fermentation
8 parts of agent, 12 parts of cavings；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：After by the pure worm high-temperature sterilization of step (2), slurries are milled into；
(4) slurry hydrolysis：To protease sealing hydrolysis is added in slurries obtained in step (3), hydrolysis time is 42h, temperature
Spend is 53 DEG C；
(5) concentrate, dry：It is dried and crushes after slurries concentration after being hydrolyzed to step (4), albumen powder is obtained；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal most 7.4% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 150 parts of water, is 1 in solid-liquid ratio:12 (W/V), microwave power is 170W, and Extracting temperature is 89 DEG C, during extraction
Between be 1.5h, then filter residue, concentration, to dry to water content be 2.6%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：180 parts of peptone, 130 parts of Yeast diffusion juice powder, 45 parts of beef extract, micro- 2 parts, 1000 parts of water, it is described micro
Element is in units of weight portion, including following raw material：4 parts of Zn-ef ficiency, 3 parts of selenium element, 2 parts of I；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 3min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, actinomyces, temperature be 32 DEG C, rotating speed be 200r/min under carry out strain fermentation 80h, the red
Nocard's bacillus ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces, the weight ratio of photosynthetic bacteria
It is 5:3:2:1；
(E) it is 2.5%, size-reduced mistake 100 to concentrate the zymotic fluid that step (D) completes strain fermentation, dry to water content
Mesh sieve, is obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1cm and temperature be 90 DEG C at dry to moisture
Content is 3.2%, is finally cooled to 25 DEG C, and the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease is obtained.
Embodiment 3
A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease, in units of weight portion, including following raw material：Albumen powder 50
Part, 5 parts of beet pulp, 6 parts of earthworm powder, 18 parts of Ipomoea batatas, 7 parts of Cottonseed Meal, 8 parts of rapeseed dregs, 14 parts of flour, 3 parts of vitamin, amino acid
2 parts, trace element 1.6 parts, 8 parts of vegetable oil, 2 parts of phagostimulant, Streptococcus iniae disease 0.9 part of biological agent.
The vitamin is composed of the following components in units of weight portion：Raw 1.2 parts of element A, vitamin B11 part, dimension life
0.8 part of plain C.
The amino acid is composed of the following components in units of weight portion：0.8 part of phenylalanine, glutamic acid 0.7 part, thread
0.5 part of propylhomoserin.
The trace element is composed of the following components in units of weight portion：0.1 part of copper, 0.1 part of manganese element, chromium
0.1 part of element, 0.45 part of ferro element, 0.45 part of Zn-ef ficiency, 0.4 part of selenium element, 0.4 part of I.
The vegetable oil is 2 parts of peanut oil, 2 parts of soybean oil, 2 parts of castor oil, 2 parts of rape seed oil.
The phagostimulant, in units of weight portion, including following raw material：18 parts of anise, 15 parts of matrimony vine, 12 parts of Poria cocos,
10 parts of peppermint, 8 parts of hawthorn, 6 parts of purslane, 6 parts of garlic, 4 parts of Chinese cassia tree, 30 parts of dimethyl-β-DMPT, 23 parts of glycine betaine,
10 parts of nucleotides, 1 part of sweetener, 0.5 part of flavouring agent, 3 parts of acid, 0.8 part of pungent agent, 0.6 part of tasty agents；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
The preparation method of the feed of the preventing and treating Tilapia mossambica Streptococcus iniae disease, comprises the following steps：
S1：Beet pulp, earthworm powder, Ipomoea batatas, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 300 mesh
Close uniform, homogeneous mixture is obtained；
S2：To in homogeneous mixture obtained in step S1 add albumen powder, flour, vitamin, amino acid, trace element,
Vegetable oil, phagostimulant, Streptococcus iniae disease biological agent, and 160 parts of water, are well mixed again, and moist forage is obtained；
The preparation method of the albumen powder, comprises the following steps：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, micro- life
Thing feed is in units of weight portion, including following raw material：48 parts of agricultural waste material, 40 parts of vegetables slag, 52 parts of water, strain fermentation
16 parts of agent, 22 parts of cavings；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：After by the pure worm high-temperature sterilization of step (2), slurries are milled into；
(4) slurry hydrolysis：To protease sealing hydrolysis is added in slurries obtained in step (3), hydrolysis time is 45h, temperature
Spend is 55 DEG C；
(5) concentrate, dry：It is dried and crushes after slurries concentration after being hydrolyzed to step (4), albumen powder is obtained；
The preparation method of phagostimulant, comprises the following steps described in step S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal most 6.5% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, the Chinese cassia tree carry out
It is pulverized and mixed, adds 300 parts of water, is 1 in solid-liquid ratio:14 (W/V), microwave power is 200W, and Extracting temperature is 94 DEG C, during extraction
Between be 1.2h, then filter residue, concentration, to dry to water content be 2.5%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, perfume (or spice) in medicinal powder obtained in step (b)
Taste agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained；
The preparation method of the Streptococcus iniae disease biological agent, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize
What 30min was carried out；
(B) to adding culture medium in the biological fermentation tank of step (A), the culture medium in units of weight portion, including with
Lower raw material：220 parts of peptone, 150 parts of Yeast diffusion juice powder, 65 parts of beef extract, micro- 4 parts, 2000 parts of water, it is described micro
Element is in units of weight portion, including following raw material：9 parts of Zn-ef ficiency, 6 parts of selenium element, 4 parts of I；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, under pressure is 1.5 kilograms
Sterilization 5min is carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, actinomyces, temperature be 36 DEG C, rotating speed be 220r/min under carry out strain fermentation 50h, the red
Nocard's bacillus ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces, the weight ratio of photosynthetic bacteria
It is 7:4:4:3；
(E) it is 2.4%, size-reduced mistake 300 to concentrate the zymotic fluid that step (D) completes strain fermentation, dry to water content
Mesh sieve, is obtained Streptococcus iniae disease biological agent；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1-0.3cm and temperature be 105 DEG C at dry to
Moisture is 3.2%, is finally cooled to 30 DEG C, and the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease is obtained.
It is the effect of the feed of checking present invention preventing and treating Tilapia mossambica Streptococcus iniae disease, by following experimental verification.
From 0.1-12kg, specification it is almost consistent and make a definite diagnosis suffer from Streptococcus iniae disease Tilapia mossambica 360, be randomly divided into
Four groups, i.e. control group, test group 1, test group 2, test group 3, every group is done 3 parallel laboratory tests, every group of Tilapia mossambica 30.Control group
Tilapia mossambica is fed with albumen powder, beet pulp, earthworm powder, Ipomoea batatas, Cottonseed Meal, rapeseed dregs, flour, vitamin, amino acid, micro unit
The basic feed that element, vegetable oil, phagostimulant are configured to, the Tilapia mossambica of test group 1-3 feeds the feeding of embodiment of the present invention 1-3 respectively
Material.Continuous feeding 10 days, is fed intake 2 times daily, experimental period 10 days, and the average daily gain amount of Tilapia mossambica, average is calculated after terminating
Day feeding amount, feed coefficient, the death rate and cure rate, as a result see the table below.
As seen from the above table, test group is contrasted with control group, and feed of the invention can be improved suffers from Streptococcus iniae disease Rofe
The feed intake and the speed of growth of fish, strengthen the immunity and anti-stress ability of Tilapia mossambica, reduce Tilapia mossambica and suffer from Streptococcus iniae disease
The death rate, improve cure rate.
The specific implementation of the invention is not to be limited to these illustrations for above content, is led for technology belonging to the present invention
For the those of ordinary skill in domain, without departing from the inventive concept of the premise, some simple deduction or replace can also be made,
The scope of patent protection that the claims of the present invention by being submitted to determine should be all considered as belonging to.
Claims (10)
1. it is a kind of to prevent and treat the sick feed of Tilapia mossambica Streptococcus iniae, it is characterised in that in units of weight portion, including following original
Material：Albumen powder 30-50 parts, beet pulp 3-5 parts, earthworm powder 4-6 parts, Ipomoea batatas 12-18 parts, Cottonseed Meal 4-7 parts, rapeseed dregs 5-8 parts,
Flour 9-14 parts, vitamin 1.8-3 parts, -2 parts of amino acid/11,1-1.8 parts of trace element, vegetable oil 5-8 parts, phagostimulant 1-2 parts,
Streptococcus iniae 0.6-0.9 parts of biological agent of disease.
2. the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease according to claim 1, it is characterised in that the vitamin with
Weight portion is unit, composed of the following components：Raw 0.8-1.2 parts of element A, vitamin B10.6-1 parts, vitamin C 0.4-0.8
Part.
3. the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease according to claim 1, it is characterised in that the amino acid with
Weight portion is unit, composed of the following components：Phenylalanine 0.5-0.8 parts, glutamic acid 0.3-0.7 parts, serine 0.2-0.5
Part.
4. it is according to claim 1 to prevent and treat the sick feed of Tilapia mossambica Streptococcus iniae, it is characterised in that the trace element
It is composed of the following components in units of weight portion：Copper 0.05-0.1 parts, manganese element 0.05-0.1 parts, chromium 0.05-
0.1 part, ferro element 0.2-0.45 parts, Zn-ef ficiency 0.2-0.45 parts, selenium element 0.25-0.3 parts, I 0.2-0.3 parts.
5. it is according to claim 1 to prevent and treat the sick feed of Tilapia mossambica Streptococcus iniae, it is characterised in that the vegetable oil bag
Include one or more in peanut oil, soybean oil, linseed oil, castor oil, rape seed oil.
It is 6. according to claim 1 to prevent and treat the sick feed of Tilapia mossambica Streptococcus iniae, it is characterised in that the phagostimulant,
In units of weight portion, including following raw material：Anise 6-18 parts, matrimony vine 5-15 parts, Poria cocos 4-12 parts, peppermint 3-10 parts, mountain
Short, bristly hair or beard 4-8 parts, purslane 2-6 parts, garlic 4-6 parts, Chinese cassia tree 2-4 parts, dimethyl-β-DMPT 20-30 parts, glycine betaine 15-23
Part, nucleotides 5-10 parts, sweetener 0.6-1 parts, flavouring agent 0.2-0.5 parts, acid 1-3 parts, it is 0.4-0.8 parts of pungent agent, fresh
0.3-0.6 parts of taste agent；
The sweetener is tripotassium glycyrrhetate；
The flavouring agent is 3- muscones；
The acid is citric acid；
The pungent agent is chilli extract；
The tasty agents is sodium guanylate.
7. the preparation method of the feed of the preventing and treating Tilapia mossambica Streptococcus iniae disease according to claim any one of 1-6, it is special
Levy and be, comprise the following steps：
S1：Beet pulp, earthworm powder, Ipomoea batatas, Cottonseed Meal, rapeseed dregs are shone into dry doubling respectively to crush, is mixed after crossing the sieve of 100-300 mesh
Close uniform, homogeneous mixture is obtained；
S2：To addition albumen powder, flour, vitamin, amino acid, trace element, plant in homogeneous mixture obtained in step S1
Oil, phagostimulant, Streptococcus iniae disease biological agent, and 100-160 parts of water, are well mixed again, and moist forage is obtained；
S3：Moist forage obtained in step S2 be made the particle of diameter 0.1-0.3cm and temperature be 90-105 DEG C at dry to
Moisture≤3.6%, is finally cooled to 25-30 DEG C, and the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease is obtained.
8. the preparation method of the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease according to claim 7, it is characterised in that step
The preparation method of albumen powder, comprises the following steps described in rapid S2：
(1) egg cultivation：The worm's ovum that the yellow meal worm of artificial feeding breeds is put into microbiological feed and is cultivated, the microorganism is raised
Material is in units of weight portion, including following raw material：Agricultural waste material 40-48 parts, vegetables slag 30-40 parts, water 45-52 parts, bacterium
Plant 8-16 parts, cavings 12-22 parts of leavening；
(2) worm material is separated：After the worm's ovum of step (1) forms pure worm, pure worm is separated with microbiological feed；
(3) pure worm defibrination：After by the pure worm high-temperature sterilization of step (2), slurries are milled into；
(4) slurry hydrolysis：To protease sealing hydrolysis is added in slurries obtained in step (3), hydrolysis time is 42-45h, temperature
It is 53-55 DEG C；
(5) concentrate, dry：It is dried and crushes after slurries concentration after being hydrolyzed to step (4), albumen powder is obtained.
9. the preparation method of the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease according to claim 6, it is characterised in that step
The preparation method of phagostimulant, comprises the following steps described in rapid S2：
A anise, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree are removed moisture removal to≤8% by ()；
B () goes step (a) anise of moisture removal, matrimony vine, Poria cocos, peppermint, hawthorn, purslane, garlic, Chinese cassia tree to be crushed
Mixing, adds 150-300 parts of water, is 1 in solid-liquid ratio:12-14 (W/V), microwave power is 170-200W, and Extracting temperature is 89-
94 DEG C, extraction time is 1.2-1.5h, then filter residue, concentration, dry to water content≤3%, prepared medicinal powder；
C () will add dimethyl-β-DMPT, glycine betaine, nucleotides, sweetener, fragrance in medicinal powder obtained in step (b)
Agent, acid, pungent agent, tasty agents, are then well mixed, and phagostimulant is obtained.
10. the preparation method of the feed of preventing and treating Tilapia mossambica Streptococcus iniae disease according to claim 6, it is characterised in that
The preparation method of the disease biological agent of Streptococcus iniae described in step S2, comprises the following steps：
(A) ullage disappears on biological fermentation tank, the sky disappear be temperature be 130 DEG C, pressure be 1.5 kilograms under sterilize 30min
Carry out；
(B) to culture medium is added in the biological fermentation tank of step (A), the culture medium is in units of weight portion, including following original
Material：Peptone 180-220 parts, 130-150 parts of Yeast diffusion juice powder, beef extract 45-65 parts, 2-4 parts of trace element, water 1000-
2000 parts, the trace element is in units of weight portion, including following raw material：Zn-ef ficiency 4-9 parts, selenium element 3-6 parts, I
2-4 parts；
(C) culture medium of step (B) is carried out it is real disappear, the reality disappear be temperature be 121 DEG C, pressure be 1.5 kilograms under sterilize
What 3min was carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C)
ACCC40100, Bacillus, actinomyces, temperature be 32-36 DEG C, rotating speed be 200-220r/min under carry out strain fermentation 50-
80h, the nocardia rubra ATCC11653, coral Nocard's bacillus ACCC40100, Bacillus, nitrifier, actinomyces, light
The weight ratio for closing bacterium is 5-7:3-4:2-4:1-3；
(E) step (D) completed into the zymotic fluid concentration of strain fermentation, dried to water content≤3%, it is size-reduced to cross 100-300 mesh
Sieve, is obtained Streptococcus iniae disease biological agent.
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| CN109007242A (en) * | 2018-08-20 | 2018-12-18 | 广州海诚生物科技有限公司 | A kind of nonreactive functional feed preventing Tilapia mossambica streptococcosis |
| CN109497345A (en) * | 2018-11-21 | 2019-03-22 | 广西南宁市武鸣明山红农业科技开发有限公司 | The feed making method for preventing and treating Tilapia mossambica Streptococcus iniae disease |
| CN109497344A (en) * | 2018-11-21 | 2019-03-22 | 广西南宁市武鸣明山红农业科技开发有限公司 | The feed for improving yield and its application in raising Tilapia mossambica |
| CN109691588A (en) * | 2018-11-21 | 2019-04-30 | 广西南宁市武鸣明山红农业科技开发有限公司 | Chinese medicine phagostimulant and its application in tilapia feed |
| CN109691587A (en) * | 2018-11-21 | 2019-04-30 | 广西南宁市武鸣明山红农业科技开发有限公司 | Improve the phagostimulant processing method of Tilapia mossambica feed intake |
| CN109699848A (en) * | 2018-11-21 | 2019-05-03 | 广西南宁市武鸣明山红农业科技开发有限公司 | The biological agent for treating Tilapia mossambica Streptococcus iniae disease |
| CN111955607A (en) * | 2020-07-30 | 2020-11-20 | 宿迁益客饲料有限公司 | Feed additive for promoting production performance of laying poultry and application thereof |
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| CN109691587A (en) * | 2018-11-21 | 2019-04-30 | 广西南宁市武鸣明山红农业科技开发有限公司 | Improve the phagostimulant processing method of Tilapia mossambica feed intake |
| CN109699848A (en) * | 2018-11-21 | 2019-05-03 | 广西南宁市武鸣明山红农业科技开发有限公司 | The biological agent for treating Tilapia mossambica Streptococcus iniae disease |
| CN111955607A (en) * | 2020-07-30 | 2020-11-20 | 宿迁益客饲料有限公司 | Feed additive for promoting production performance of laying poultry and application thereof |
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