CN109691588A - Chinese medicine phagostimulant and its application in tilapia feed - Google Patents

Chinese medicine phagostimulant and its application in tilapia feed Download PDF

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CN109691588A
CN109691588A CN201811391494.5A CN201811391494A CN109691588A CN 109691588 A CN109691588 A CN 109691588A CN 201811391494 A CN201811391494 A CN 201811391494A CN 109691588 A CN109691588 A CN 109691588A
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parts
chinese medicine
feed
medicine phagostimulant
agent
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韦红
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Guangxi Nanning City Wuming Red Mountain Agricultural Science And Technology Development Co Ltd
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Guangxi Nanning City Wuming Red Mountain Agricultural Science And Technology Development Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a kind of Chinese medicine phagostimulants, it is characterized in that, as unit of parts by weight, including following raw material: 8-12 parts of anise, 7-13 parts of Cortex Magnoliae Officinalis, 9-14 parts of radix pseudostellariae, 4-7 parts of peppermint, 5-10 parts of granatum, 2-5 parts of hawthorn, 3-6 parts of cortex cinnamomi, 2-6 parts of Radix Notoginseng, 4-12 parts of dried orange peel, 7-13 parts of glycine betaine, 1.2-1.8 parts of sweetener, 0.6-1 parts of flavouring agent, 0.5-0.9 parts of acid, 1-1.6 parts of pungent agent, 0.7-1.2 parts of tasty agents.Chinese medicine phagostimulant of the invention can effectively facilitate Tilapia mossambica to the abilities of digestive and absorption of feed, improve Tilapia mossambica to the grazing rate and palatability of indigestibility feed, expand the use scope and usage amount of unconventional feed, reduce feed cost, improve productivity effect.

Description

Chinese medicine phagostimulant and its application in tilapia feed
Technical field
The invention belongs to technique for producing feed fields, and in particular to a kind of Chinese medicine phagostimulant and its in tilapia feed Using.
Background technique
In the production process of biological feedstuff, addition is common insect albumen powder, the production work of common insect albumen powder It is to use wheat bran, green vegetables as culture to cultivate worm's ovum, after so that worm's ovum is grown up to pure worm, to pure worm fried dry or microwave in skill Baking oven is dry, is then comminuted into worm powder, then worm powder is mixed with culture or fermented bean dregs or general proteins raw material, is formed common Insect albumen powder, in the production technology, since the culture cultivated worm's ovum is wheat bran, green vegetables, therefore the pure worm grown up to Ingredient in be free of antibacterial peptide and saccharomycete, therefore do not have improve immunity the effect of；Due to using the drying to pure worm fried dry Mode is easy to make protein denaturation, to destroy due nutritional ingredient；Due to crushing pure worm to form worm powder, do not make water Solution processing, ingredient exists in the form of the protein of intrinsic macromolecular or in the form of polypeptide, therefore is not easy to be absorbed.
Phagostimulant has obvious and positive biological action to the raising of Tilapia mossambica, can improve the attractant of feed and fit Mouth property, improves food conversion ratio, and then improves the production performance of Tilapia mossambica comprehensively, such as increases feed intake, improves daily gain, improvement Meat quality etc..One side phagostimulant can cover the bad smell in feed by special odor, stimulate the feeling of Tilapia mossambica Organ, to achieve the purpose that promote Tilapia mossambica to ingest, accelerate Growth Op Tilapia；On the other hand, Tilapia mossambica is induced by phagostimulant It ingests, shortens the time of ingesting, improve the utilization rate of feed.Phagostimulant currently on the market is largely flavouring agent or sweet taste Agent, effect is more single, can only smell to Tilapia mossambica or vision or the sense of taste etc. form single stimulation；And some flavouring agents or It is obvious to generate medicament residue, toxic side effect from chemically synthesized product easily in Tilapia mossambica body for the constituent of sweetener.Cause How these single Attractants, is compounded to form that effect is obvious, quality is stable and economical and practical phagostimulant is trend by this Place.
Streptococcus is widely present in nature, people and animal, causes various purulent inflammations, scarlet fever, erysipelas, newborn Septicemia, meningitis, puerperal fever and streptococcus allergic disease etc..Aquiculture animal can also infect streptococcus Streptococcosis causes massive mortality, causes great economic loss to World Aquaculture every year.China is that aquaculture is big State, every year because of streptococcosis to aquaculture caused by about 1,000,000,000 dollars of direct economic loss.Tilapia mossambica be important the torrid zone and One of subtropical zone aquiculture animal and the main object of streptococcal infection.Start within 2001, Chinese Rofe fish culture is successively There is the infection of streptococcosis, and causes the high mortality of 30%-50% in individual farms.2009-2013 continuous 5 years, Streptococcosis is in Chinese Rofe fish culture large area outbreak of epidemic, and diseased region expands year by year, morbidity and mortality are passed year by year Increase, susceptible Tilapia mossambica specification limit expands year by year.By the average annual Production rate of China Tilapia mossambica, estimate annual streptococcosis to China Direct economic loss caused by Tilapia mossambica industry is as high as 400,000,000 dollars.Disease is broken out and bring product safety problem therewith Have become the bottleneck for restricting Tilapia mossambica industry development.Lack a set of effective scheme at present and prevents and treats the disease, drug It can only play the role of controlling the complementary of the state of an illness in early days, but with the generation and accumulation of drug resistance and drug resistance, the status of the disease Almost " pasting medical help can prevent ".
With the increase of market demand, the outburst of various diseases causes tremendous influence to Tilapia mossambica industry, therefore Being effectively prevented and treated for Tilapia mossambica disease, especially streptococcosis how is effectively prevented, yield is improved, increases industry size, Developing Tilapia mossambica healthy aquaculture technology has had very big necessity and urgency.
Chinese patent application document " a kind of Tilapia mossambica high-yield and high-efficiency healthy aquaculture feed and preparation method thereof (application number: 201611082551.2) a kind of Tilapia mossambica high-yield and high-efficiency healthy aquaculture feed is disclosed, as unit of parts by weight, including it is following Raw material: 30-50 parts of albumen powder, 10-18 parts of Soybean Meal, 6-12 parts of peanut meal, 8-14 parts of corn, 4-7 parts of Cottonseed Meal, rapeseed dregs 5- 8 parts, 10-16 parts of Strong flour, 1.8-3 parts of vitamin, -2 parts of amino acid 1,1-1.8 parts of microelement, 5-8 parts of vegetable oil, lure Agent 1-2 part, 0.6-0.9 parts of Streptococcus iniae disease biological agent are eaten, the feed is walked by drying, crushing, mix, dry etc. Made of rapid.The feed formulation is reasonable, full of nutrition, but there is a problem that raising average growth rate and cure rate are lower, Middle average growth rate and cure rate are respectively 138.01%-146.29% and 92.22%-93.33%, are unfavorable for economic raising.Cause How this, develop a set of the relevant technologies for more improving Tilapia mossambica average growth rate and cure rate, become Development Trend.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Chinese medicine phagostimulant and its application in tilapia feed, with Solve the problems, such as phagostimulant in the prior art containing any antibiotic cause not exclusively, effect it is more single.
In order to solve the above technical problems, the invention adopts the following technical scheme:
A kind of Chinese medicine phagostimulant, as unit of parts by weight, including following raw material: 8-12 parts of anise, 7-13 parts of Cortex Magnoliae Officinalis, too Son ginseng 9-14 parts, 4-7 parts of peppermint, 5-10 parts of granatum, 2-5 parts of hawthorn, 3-6 parts of cortex cinnamomi, 2-6 parts of Radix Notoginseng, 4-12 parts of dried orange peel, sweet tea 7-13 parts of dish alkali, 1.2-1.8 parts of sweetener, 0.6-1 parts of flavouring agent, 0.5-0.9 parts of acid, 1-1.6 parts of pungent agent, tasty agents 0.7-1.2 parts.
Further, the Chinese medicine phagostimulant, as unit of parts by weight, including following raw material: 10 parts of anise, Cortex Magnoliae Officinalis 12 parts, 13 parts of radix pseudostellariae, 6 parts of peppermint, 9 parts of granatum, 4 parts of hawthorn, 5 parts of cortex cinnamomi, 5 parts of Radix Notoginseng, 10 parts of dried orange peel, glycine betaine 12 Part, 1.6 parts of sweetener, 0.9 part of flavouring agent, 0.7 part of acid, 1.2 parts of pungent agent, 0.8 part of tasty agents.
Further, the sweetener is sodium cyclohexylsulfamate.
Further, the flavouring agent is ambrein.
Further, the acid is malic acid.
Further, the pungent agent is allicin.
Further, the tasty agents is Pidolidone.
The present invention also provides a kind of application of Chinese medicine phagostimulant in tilapia feed, the tilapia feeds, with weight Amount part is unit, including following raw material: 22-28 parts of pure worm slurry protein powder, 12-20 parts of Soybean Meal, 8-15 parts of tapioca, corn 6-10 parts of powder, 3-5 parts of Cottonseed Meal, 4-8 parts of rapeseed dregs, 1.5-2.8 parts of vitamin, 2- amino -4- carbamyl butyric acid 1.2-2.5 Part, 0.8-1.5 parts of non-starch polysaccharide enzyme, 2-3 parts of microorganism formulation, 2.2-3 parts of Chinese medicine phagostimulant, Streptococcus iniae diease occurrence object system 1-1.5 parts of agent, I 0.9-1.2 parts of additive, II 1-1.8 parts of additive, III 0.6-0.9 parts of additive.
Further, the vitamin is made of following raw material as unit of parts by weight: raw 0.8 part of element A, vitamin 0.6 part of B1,0.4 part of vitamin C.
Further, the microorganism formulation is bacillus subtilis.
The invention has the following advantages:
(1) Chinese medicine phagostimulant of the invention can effectively improve Tilapia mossambica feed intake and the speed of growth, shorten growth cycle；
(2) Chinese medicine phagostimulant of the invention can effectively facilitate Tilapia mossambica to the abilities of digestive and absorption of feed, improve Tilapia mossambica to difficulty The grazing rate and palatability for digesting feed expand the use scope and usage amount of unconventional feed, reduce feed cost, mention High productivity effect.
[specific embodiment]
It to facilitate a better understanding of the present invention, is illustrated by following embodiment, these embodiments belong to protection of the invention Range, but do not limit the scope of the invention.
In embodiment, the tilapia feed, as unit of parts by weight, including following raw material: pure worm slurry protein powder 22- 28 parts, 12-20 parts of Soybean Meal, 8-15 parts of tapioca, 6-10 parts of corn flour, 3-5 parts of Cottonseed Meal, 4-8 parts of rapeseed dregs, vitamin 1.5-2.8 parts, 1.2-2.5 parts of 2- amino -4- carbamyl butyric acid, 0.8-1.5 parts of non-starch polysaccharide enzyme, microorganism formulation 2-3 Part, 2.2-3 parts of Chinese medicine phagostimulant, 1-1.5 parts of Streptococcus iniae disease biological agent, I 0.9-1.2 parts of additive, additive II 1-1.8 parts, III 0.6-0.9 parts of additive；
The vitamin is made of following raw material as unit of parts by weight: raw 0.8 part of element A, vitamin B1 0.6 part, dimension life 0.4 part of plain C；
The non-starch polysaccharide enzyme is mannase；
The microorganism formulation is bacillus subtilis；
The Chinese medicine phagostimulant, as unit of parts by weight, including following raw material: 8-12 parts of anise, 7-13 parts of Cortex Magnoliae Officinalis, radix pseudostellariae 9-14 parts, 4-7 parts of peppermint, 5-10 parts of granatum, 2-5 parts of hawthorn, 3-6 parts of cortex cinnamomi, 2-6 parts of Radix Notoginseng, 4-12 parts of dried orange peel, glycine betaine 7-13 parts, 1.2-1.8 parts of sweetener, 0.6-1 parts of flavouring agent, 0.5-0.9 parts of acid, 1-1.6 parts of pungent agent, tasty agents 0.7- 1.2 part；
The sweetener is sodium cyclohexylsulfamate；
The flavouring agent is ambrein；
The acid is malic acid；
The pungent agent is allicin；
The tasty agents is Pidolidone；
The additive I is made of following raw material as unit of parts by weight: 12-20 parts of thiocarbamic acid magnesium, dimethyl disulfide For 5-8 parts of potassium carbamate, 2-4 parts of aminoquinoxaline；
The additive II is made of following raw material as unit of parts by weight: 4-9 parts of potassium pyrophosphate, 7-10 parts of sodium stearate, brown 2-5 parts of mosanom；
The additive III is made of following raw material as unit of parts by weight: 5-10 parts of 2- Amino-4-nitrophenol sodium, 5- nitre 3-7 parts of base guaiacol sodium；
The preparation method of the tilapia feed, comprising the following steps:
S1: shining dry doubling for Soybean Meal, Cottonseed Meal, rapeseed dregs respectively and crush, and is uniformly mixed, is made after crossing the sieve of 100-300 mesh Homogeneous mixture；
S2: pure worm slurry protein powder, tapioca, corn flour, vitamin, 2- ammonia are added into homogeneous mixture made from step S1 Base -4- carbamyl butyric acid, microorganism formulation, Chinese medicine phagostimulant, Streptococcus iniae disease biological agent, adds non-starch polysaccharide enzyme Add agent I, additive II, additive III and 210-350 parts of water, be uniformly mixed again, moist forage is made；
The preparation method of the pure worm slurry protein powder, comprising the following steps:
(1) egg cultivation: the worm's ovum that the yellow meal worm of artificial feeding breeds being put into microbiological feed and is cultivated, and the microorganism is raised Material is as unit of parts by weight, including following raw material: corn stover minces 23-37 part, 20-25 parts of maize pulp, 36-48 parts of water, feeding Leavening 3-5 parts, 8-15 parts of cavings of material；
(2) worm material separates: after the worm's ovum of step (1) forms pure worm, pure worm being separated with microbiological feed；
(3) pure worm defibrination: by the pure worm 263-331g of step (2), by material ratio 1:2.4-3.2(W/V) plus water grinding, in 64- Sterilize 25-30min at 70 DEG C, is cooled to 34-40 DEG C, and slurries are made；
(4) slurry hydrolyzes: cysteine proteinase 0.18-0.22g being added into slurries made from step (3), adjusting pH value is 5-5.4 hydrolyzes 40-43h at 43-45 DEG C, and then sterilize at 65-68 DEG C 28-32min, centrifugal filtration, and hydrolyzate is made；
(5) hydrolyzate ultrafiltration: the hollow-fibre membrane for the use of molecular cut off being 7000-8000 by hydrolyzate made from step (4) Ultrafiltrate is made in ultrafiltration；
(6) dry, crushing, sieving: ultrafiltrate made from step (5) being freeze-dried, 200-300 mesh is smashed it through, Pure worm slurry protein powder is made；
The preparation method of Chinese medicine phagostimulant described in step S2, comprising the following steps:
(a) go moisture removal to≤6% anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, Radix Notoginseng, dried orange peel；
(b) step (a) is gone to anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, the Radix Notoginseng, dried orange peel of moisture removal It is pulverized and mixed, 200-320 parts of water is added, is 1:15-18(W/V in solid-liquid ratio), microwave power 250-300W extracts temperature Degree is 92-95 DEG C, extraction time 0.9-1.3h, and then filter residue, concentration, drying to water content≤3%, are made medicinal powder；
(c) glycine betaine, sweetener, flavouring agent, acid, pungent agent, tasty agents will be added in medicinal powder made from step (b), so After be uniformly mixed, be made Chinese medicine phagostimulant；
The preparation method of the Streptococcus iniae disease biological agent, comprising the following steps:
(A) ullage disappears on biological fermentation tank, and it is to sterilize 25min in the case where temperature is 135 DEG C, pressure is 1.6 kilograms that the sky, which disappears, It carries out；
(B) culture medium is added into the biological fermentation tank of step (A), the culture medium is as unit of parts by weight, including following original Material: 120-160 parts of corn protein powder, 70-110 parts of Yeast diffusion juice powder, 20-40 parts of beef extract, non-starch polysaccharide enzyme 1-1.8 Part, 3-6 parts of dipotassium hydrogen phosphate, 2-5 parts of ferrous sulfate heptahydrate, 7-10 parts of epsom salt, 5-8 parts of calcium acetate, water 800-1500 Part, the non-starch polysaccharide enzyme is as unit of parts by weight, including following raw material: 3-6 parts of cellulase, 4-9 parts of 1,4 beta-glucanase, 2-5 parts of zytase；
(C) culture medium of step (B) disappear in fact, the reality, which disappears, to be sterilized in the case where temperature is 125 DEG C, pressure is 1.6 kilograms What 2min was carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C) ACCC40100 is 30-36 DEG C in temperature, and revolving speed is that strain fermentation 40-60h is carried out under 200-250r/min, the red promise card Salmonella ATCC11653, coral Nocard's bacillus ACCC40100 weight ratio be 8-12:5-7；
(E) step (D) fermentation liquid for completing strain fermentation is concentrated, is dry to water content≤4%, crushed 100-200 mesh Streptococcus iniae disease biological agent is made in son；
Moist forage made from step S2: being made the particle of diameter 0.1-0.3cm by S3 and is dried at being 95-102 DEG C in temperature Moisture content≤3% is finally cooled to 25-30 DEG C, and tilapia feed is made.
It is illustrated below by more specific embodiment.
Embodiment 1
A kind of tilapia feed, as unit of parts by weight, including following raw material: 25 parts of pure worm slurry protein powder, Soybean Meal 12-20 Part, 10 parts of tapioca, 9 parts of corn flour, 4 parts of Cottonseed Meal, 6 parts of rapeseed dregs, 2.3 parts of vitamin, 2- amino -4- carbamyl fourth Sour 1.8 parts, 1.2 parts of non-starch polysaccharide enzyme, 2.4 parts of microorganism formulation, 2.7 parts of Chinese medicine phagostimulant, Streptococcus iniae diease occurrence object system 1.2 parts of agent, I 1 parts of additive, II 1.3 parts of additive, III 0.89 parts of additive；
The vitamin is made of following raw material as unit of parts by weight: raw 0.8 part of element A, vitamin B1 0.6 part, dimension life 0.4 part of plain C；
The non-starch polysaccharide enzyme is mannase；
The microorganism formulation is bacillus subtilis；
The Chinese medicine phagostimulant, as unit of parts by weight, including following raw material: 10 parts of anise, 12 parts of Cortex Magnoliae Officinalis, radix pseudostellariae 13 Part, 6 parts of peppermint, 9 parts of granatum, 4 parts of hawthorn, 5 parts of cortex cinnamomi, 5 parts of Radix Notoginseng, 10 parts of dried orange peel, 12 parts of glycine betaine, 1.6 parts of sweetener, 0.9 part of flavouring agent, 0.7 part of acid, 1.2 parts of pungent agent, 0.8 part of tasty agents；
The sweetener is sodium cyclohexylsulfamate；
The flavouring agent is ambrein；
The acid is malic acid；
The pungent agent is allicin；
The tasty agents is Pidolidone；
The additive I is made of following raw material as unit of parts by weight: 16 parts of thiocarbamic acid magnesium, dimethyl disulfide generation 7 parts of potassium carbamate, 3 parts of aminoquinoxaline；
The additive II is made of following raw material as unit of parts by weight: 8 parts of potassium pyrophosphate, 9 parts of sodium stearate, alginic acid 4 parts of sodium；
The additive III is made of following raw material as unit of parts by weight: 8 parts of 2- Amino-4-nitrophenol sodium, 5- nitro are cured Create wooden 6 parts of sodium of the phenol；
The preparation method of the tilapia feed, comprising the following steps:
S1: shining dry doubling for Soybean Meal, Cottonseed Meal, rapeseed dregs respectively and crush, and is uniformly mixed after crossing the sieve of 200 mesh, is made uniform Mixture；
S2: pure worm slurry protein powder, tapioca, corn flour, vitamin, 2- ammonia are added into homogeneous mixture made from step S1 Base -4- carbamyl butyric acid, microorganism formulation, Chinese medicine phagostimulant, Streptococcus iniae disease biological agent, adds non-starch polysaccharide enzyme Add agent I, additive II, additive III and 280 parts of water, be uniformly mixed again, moist forage is made；
The preparation method of the pure worm slurry protein powder, comprising the following steps:
(1) egg cultivation: the worm's ovum that the yellow meal worm of artificial feeding breeds being put into microbiological feed and is cultivated, and the microorganism is raised Material is as unit of parts by weight, including following raw material: corn stover minces 32 parts, 24 parts of maize pulp, 42 parts of water, feed fermentation agent 4 Part, 12 parts of cavings；
(2) worm material separates: after the worm's ovum of step (1) forms pure worm, pure worm being separated with microbiological feed；
(3) pure worm defibrination: the pure worm 295g of step (2) is sterilized at 68 DEG C by material ratio 1:2.6(W/V) plus water grinding 27min is cooled to 35 DEG C, and slurries are made；
(4) slurry hydrolyzes: cysteine proteinase 0.2g is added into slurries made from step (3), adjusting pH value is 5.3, 42h is hydrolyzed at 44 DEG C, then sterilize at 67 DEG C 29min, centrifugal filtration, and hydrolyzate is made；
(5) hydrolyzate ultrafiltration: the hollow-fibre membrane ultrafiltration for the use of molecular cut off being 7000 by hydrolyzate made from step (4), Ultrafiltrate is made；
(6) dry, crushing, sieving: ultrafiltrate made from step (5) being freeze-dried, 200 meshes are smashed it through, and is made Pure worm slurry protein powder；
The preparation method of Chinese medicine phagostimulant described in step S2, comprising the following steps:
(a) go moisture removal to 6% anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, Radix Notoginseng, dried orange peel；
(b) step (a) is gone to anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, the Radix Notoginseng, dried orange peel of moisture removal It is pulverized and mixed, 270 parts of water is added, is 1:17(W/V in solid-liquid ratio), microwave power 280W, Extracting temperature is 93 DEG C, is mentioned Taking the time is 1.1h, and then filter residue, concentration, drying to water content are 3%, and medicinal powder is made；
(c) glycine betaine, sweetener, flavouring agent, acid, pungent agent, tasty agents will be added in medicinal powder made from step (b), so After be uniformly mixed, be made Chinese medicine phagostimulant；
The preparation method of the Streptococcus iniae disease biological agent, comprising the following steps:
(A) ullage disappears on biological fermentation tank, and it is to sterilize 25min in the case where temperature is 135 DEG C, pressure is 1.6 kilograms that the sky, which disappears, It carries out；
(B) culture medium is added into the biological fermentation tank of step (A), the culture medium is as unit of parts by weight, including following original Material: 150 parts of corn protein powder, 90 parts of Yeast diffusion juice powder, 30 parts of beef extract, 1.5 parts of non-starch polysaccharide enzyme, dipotassium hydrogen phosphate 5 Part, 4 parts of ferrous sulfate heptahydrate, 8 parts of epsom salt, 7 parts of calcium acetate, 1200 parts of water, the non-starch polysaccharide enzyme is with parts by weight For unit, including following raw material: 5 parts of cellulase, 5 parts of 1,4 beta-glucanase, 4 parts of zytase；
(C) culture medium of step (B) disappear in fact, the reality, which disappears, to be sterilized in the case where temperature is 125 DEG C, pressure is 1.6 kilograms What 2min was carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C) ACCC40100 is 35 DEG C in temperature, and revolving speed is that strain fermentation 50h, the nocardia rubra are carried out under 230r/min ATCC11653, coral Nocard's bacillus ACCC40100 weight ratio be 10:7；
(E) step (D) is completed fermentation liquid concentration, the drying to water content of strain fermentation is 4%, crushed 100 mesh sieve, Streptococcus iniae disease biological agent is made；
Moist forage made from step S2: being made the particle of diameter 0.2cm by S3 and is dried to moisture content at being 100 DEG C in temperature It is 3%, is finally cooled to 28 DEG C, tilapia feed is made.
Embodiment 2
A kind of tilapia feed, as unit of parts by weight, including following raw material: 22 parts of pure worm slurry protein powder, 20 parts of Soybean Meal, wood 8 parts of sweet potato starch, 6 parts of corn flour, 3 parts of Cottonseed Meal, 4 parts of rapeseed dregs, 2.8 parts of vitamin, 2- amino -4- carbamyl butyric acid 1.2 Part, 0.8 part of non-starch polysaccharide enzyme, 2 parts of microorganism formulation, 2.2 parts of Chinese medicine phagostimulant, 1.5 parts of Streptococcus iniae disease biological agent, I 0.9 parts of additive, II 1.8 parts of additive, III 0.6 parts of additive；
The vitamin is made of following raw material as unit of parts by weight: raw 0.8 part of element A, vitamin B1 0.6 part, dimension life 0.4 part of plain C；
The non-starch polysaccharide enzyme is mannase；
The microorganism formulation is bacillus subtilis；
The Chinese medicine phagostimulant, as unit of parts by weight, including following raw material: 8 parts of anise, 7 parts of Cortex Magnoliae Officinalis, 14 parts of radix pseudostellariae, 4 parts of peppermint, 5 parts of granatum, 2 parts of hawthorn, 3 parts of cortex cinnamomi, 2 parts of Radix Notoginseng, 12 parts of dried orange peel, 7 parts of glycine betaine, 1.2 parts of sweetener, fragrance 0.6 part of agent, 0.5 part of acid, 1 part of pungent agent, 1.2 parts of tasty agents；
The sweetener is sodium cyclohexylsulfamate；
The flavouring agent is ambrein；
The acid is malic acid；
The pungent agent is allicin；
The tasty agents is Pidolidone；
The additive I is made of following raw material as unit of parts by weight: 12 parts of thiocarbamic acid magnesium, dimethyl disulfide generation 5 parts of potassium carbamate, 2 parts of aminoquinoxaline；
The additive II is made of following raw material as unit of parts by weight: 9 parts of potassium pyrophosphate, 7 parts of sodium stearate, alginic acid 2 parts of sodium；
The additive III is made of following raw material as unit of parts by weight: 5 parts of 2- Amino-4-nitrophenol sodium, 5- nitro are cured Create wooden 7 parts of sodium of the phenol；
The preparation method of the tilapia feed, comprising the following steps:
S1: shining dry doubling for Soybean Meal, Cottonseed Meal, rapeseed dregs respectively and crush, and is uniformly mixed after crossing the sieve of 100 mesh, is made uniform Mixture；
S2: pure worm slurry protein powder, tapioca, corn flour, vitamin, 2- ammonia are added into homogeneous mixture made from step S1 Base -4- carbamyl butyric acid, microorganism formulation, Chinese medicine phagostimulant, Streptococcus iniae disease biological agent, adds non-starch polysaccharide enzyme Add agent I, additive II, additive III and 210 parts of water, be uniformly mixed again, moist forage is made；
The preparation method of the pure worm slurry protein powder, comprising the following steps:
(1) egg cultivation: the worm's ovum that the yellow meal worm of artificial feeding breeds being put into microbiological feed and is cultivated, and the microorganism is raised Material is as unit of parts by weight, including following raw material: corn stover minces 23 parts, 25 parts of maize pulp, 36 parts of water, feed fermentation agent 3 Part, 15 parts of cavings；
(2) worm material separates: after the worm's ovum of step (1) forms pure worm, pure worm being separated with microbiological feed；
(3) pure worm defibrination: the pure worm 331g of step (2) is sterilized at 64 DEG C by material ratio 1:2.8(W/V) plus water grinding 30min is cooled to 40 DEG C, and slurries are made；
(4) slurry hydrolyzes: cysteine proteinase 0.19g is added into slurries made from step (3), adjusting pH value is 5.4, 43h is hydrolyzed at 43 DEG C, then sterilize at 65 DEG C 32min, centrifugal filtration, and hydrolyzate is made；
(5) hydrolyzate ultrafiltration: the hollow-fibre membrane ultrafiltration for the use of molecular cut off being 7000 by hydrolyzate made from step (4), Ultrafiltrate is made；
(6) dry, crushing, sieving: ultrafiltrate made from step (5) being freeze-dried, 300 meshes are smashed it through, and is made Pure worm slurry protein powder；
The preparation method of Chinese medicine phagostimulant described in step S2, comprising the following steps:
(a) go moisture removal to 5% anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, Radix Notoginseng, dried orange peel；
(b) step (a) is gone to anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, the Radix Notoginseng, dried orange peel of moisture removal It is pulverized and mixed, 200 parts of water is added, is 1:18(W/V in solid-liquid ratio), microwave power 250W, Extracting temperature is 95 DEG C, is mentioned Taking the time is 0.9h, and then filter residue, concentration, drying to water content are 3%, and medicinal powder is made；
(c) glycine betaine, sweetener, flavouring agent, acid, pungent agent, tasty agents will be added in medicinal powder made from step (b), so After be uniformly mixed, be made Chinese medicine phagostimulant；
The preparation method of the Streptococcus iniae disease biological agent, comprising the following steps:
(A) ullage disappears on biological fermentation tank, and it is to sterilize 25min in the case where temperature is 135 DEG C, pressure is 1.6 kilograms that the sky, which disappears, It carries out；
(B) culture medium is added into the biological fermentation tank of step (A), the culture medium is as unit of parts by weight, including following original Material: 120 parts of corn protein powder, 70 parts of Yeast diffusion juice powder, 20 parts of beef extract, 1.8 parts of non-starch polysaccharide enzyme, dipotassium hydrogen phosphate 3 Part, 2 parts of ferrous sulfate heptahydrate, 7 parts of epsom salt, 8 parts of calcium acetate, 800 parts of water, the non-starch polysaccharide enzyme are with parts by weight Unit, including following raw material: 3 parts of cellulase, 4 parts of 1,4 beta-glucanase, 2 parts of zytase；
(C) culture medium of step (B) disappear in fact, the reality, which disappears, to be sterilized in the case where temperature is 125 DEG C, pressure is 1.6 kilograms What 2min was carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C) ACCC40100 is 30-36 DEG C in temperature, and revolving speed is that strain fermentation 60h, the nocardia rubra are carried out under 200r/min ATCC11653, coral Nocard's bacillus ACCC40100 weight ratio be 8:5；
(E) step (D) is completed fermentation liquid concentration, the drying to water content of strain fermentation is 4%, crushed 100 mesh sieve, Streptococcus iniae disease biological agent is made；
Moist forage made from step S2: being made the particle of diameter 0.1cm by S3 and is dried to moisture content at being 102 DEG C in temperature It is 2.6%, is finally cooled to 25 DEG C, tilapia feed is made.
Embodiment 3
A kind of tilapia feed, as unit of parts by weight, including following raw material: 28 parts of pure worm slurry protein powder, 20 parts of Soybean Meal, wood 8 parts of sweet potato starch, 10 parts of corn flour, 5 parts of Cottonseed Meal, 4 parts of rapeseed dregs, 1.5 parts of vitamin, 2- amino -4- carbamyl butyric acid 1.2 Part, 0.8 part of non-starch polysaccharide enzyme, 3 parts of microorganism formulation, 3 parts of Chinese medicine phagostimulant, 1 part of Streptococcus iniae disease biological agent, addition I 0.9 parts of agent, II 1.8 parts of additive, III 0.9 parts of additive；
The vitamin is made of following raw material as unit of parts by weight: raw 0.8 part of element A, vitamin B1 0.6 part, dimension life 0.4 part of plain C；
The non-starch polysaccharide enzyme is mannase；
The microorganism formulation is bacillus subtilis；
The Chinese medicine phagostimulant, as unit of parts by weight, including following raw material: 12 parts of anise, 13 parts of Cortex Magnoliae Officinalis, radix pseudostellariae 14 Part, 7 parts of peppermint, 5 parts of granatum, 2 parts of hawthorn, 3 parts of cortex cinnamomi, 2 parts of Radix Notoginseng, 12 parts of dried orange peel, 13 parts of glycine betaine, 1.8 parts of sweetener, 1 part of flavouring agent, 0.9 part of acid, 1.6 parts of pungent agent, 1.2 parts of tasty agents；
The sweetener is sodium cyclohexylsulfamate；
The flavouring agent is ambrein；
The acid is malic acid；
The pungent agent is allicin；
The tasty agents is Pidolidone；
The additive I is made of following raw material as unit of parts by weight: 12 parts of thiocarbamic acid magnesium, dimethyl disulfide generation 5 parts of potassium carbamate, 2 parts of aminoquinoxaline；
The additive II is made of following raw material as unit of parts by weight: 4 parts of potassium pyrophosphate, 10 parts of sodium stearate, alginic acid 2 parts of sodium；
The additive III is made of following raw material as unit of parts by weight: 10 parts of 2- Amino-4-nitrophenol sodium, 5- nitro 3 parts of guaiacol sodium；
The preparation method of the tilapia feed, comprising the following steps:
S1: shining dry doubling for Soybean Meal, Cottonseed Meal, rapeseed dregs respectively and crush, and is uniformly mixed after crossing the sieve of 300 mesh, is made uniform Mixture；
S2: pure worm slurry protein powder, tapioca, corn flour, vitamin, 2- ammonia are added into homogeneous mixture made from step S1 Base -4- carbamyl butyric acid, microorganism formulation, Chinese medicine phagostimulant, Streptococcus iniae disease biological agent, adds non-starch polysaccharide enzyme Add agent I, additive II, additive III and 210 parts of water, be uniformly mixed again, moist forage is made；
The preparation method of the pure worm slurry protein powder, comprising the following steps:
(1) egg cultivation: the worm's ovum that the yellow meal worm of artificial feeding breeds being put into microbiological feed and is cultivated, and the microorganism is raised Material is as unit of parts by weight, including following raw material: corn stover minces 37 parts, 20 parts of maize pulp, 36 parts of water, feed fermentation agent 5 Part, 12 parts of cavings；
(2) worm material separates: after the worm's ovum of step (1) forms pure worm, pure worm being separated with microbiological feed；
(3) pure worm defibrination: the pure worm 331g of step (2) is sterilized at 65 DEG C by material ratio 1:2.4(W/V) plus water grinding 30min is cooled to 34 DEG C, and slurries are made；
(4) slurry hydrolyzes: cysteine proteinase 0.18g is added into slurries made from step (3), adjusting pH value is 5.4, 43h is hydrolyzed at 43 DEG C, then sterilize at 65 DEG C 32min, centrifugal filtration, and hydrolyzate is made；
(5) hydrolyzate ultrafiltration: the hollow-fibre membrane ultrafiltration for the use of molecular cut off being 7000 by hydrolyzate made from step (4), Ultrafiltrate is made；
(6) dry, crushing, sieving: ultrafiltrate made from step (5) being freeze-dried, 200 meshes are smashed it through, and is made Pure worm slurry protein powder；
The preparation method of Chinese medicine phagostimulant described in step S2, comprising the following steps:
(a) go moisture removal to 4% anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, Radix Notoginseng, dried orange peel；
(b) step (a) is gone to anise, Cortex Magnoliae Officinalis, radix pseudostellariae, peppermint, granatum, hawthorn, cortex cinnamomi, the Radix Notoginseng, dried orange peel of moisture removal It is pulverized and mixed, 320 parts of water is added, is 1:17(W/V in solid-liquid ratio), microwave power 280W, Extracting temperature is 94 DEG C, is mentioned Taking the time is 1.2h, and then filter residue, concentration, drying to water content are 3%, and medicinal powder is made；
(c) glycine betaine, sweetener, flavouring agent, acid, pungent agent, tasty agents will be added in medicinal powder made from step (b), so After be uniformly mixed, be made Chinese medicine phagostimulant；
The preparation method of the Streptococcus iniae disease biological agent, comprising the following steps:
(A) ullage disappears on biological fermentation tank, and it is to sterilize 25min in the case where temperature is 135 DEG C, pressure is 1.6 kilograms that the sky, which disappears, It carries out；
(B) culture medium is added into the biological fermentation tank of step (A), the culture medium is as unit of parts by weight, including following original Material: 160 parts of corn protein powder, 110 parts of Yeast diffusion juice powder, 20 parts of beef extract, 1 part of non-starch polysaccharide enzyme, dipotassium hydrogen phosphate 6 Part, 5 parts of ferrous sulfate heptahydrate, 10 parts of epsom salt, 5 parts of calcium acetate, 1500 parts of water, the non-starch polysaccharide enzyme is with parts by weight For unit, including following raw material: 6 parts of cellulase, 4 parts of 1,4 beta-glucanase, 5 parts of zytase；
(C) culture medium of step (B) disappear in fact, the reality, which disappears, to be sterilized in the case where temperature is 125 DEG C, pressure is 1.6 kilograms What 2min was carried out；
(D) nocardia rubra ATCC11653, coral Nocard's bacillus are accessed in the culture medium sterilized to step (C) ACCC40100 is 36 DEG C in temperature, and revolving speed is that strain fermentation 60h, the nocardia rubra are carried out under 200r/min ATCC11653, coral Nocard's bacillus ACCC40100 weight ratio be 12:5；
(E) step (D) is completed fermentation liquid concentration, the drying to water content of strain fermentation is 4%, crushed 200 mesh sieve, Streptococcus iniae disease biological agent is made；
Moist forage made from step S2: being made the particle of diameter 0.3cm by S3 and is dried to moisture content at being 95 DEG C in temperature It is 2.3%, is finally cooled to 28 DEG C, tilapia feed is made.
Comparative example 1
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare in the raw material of tilapia feed and lack additive I, additive II, additive III.
Comparative example 2
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare in the raw material of tilapia feed and lack additive Ⅰ。
Comparative example 3
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare in the raw material of tilapia feed and lack additive Ⅱ。
Comparative example 4
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare in the raw material of tilapia feed and lack additive Ⅲ。
Comparative example 5
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack pure worm slurry in the raw material of tilapia feed Albumen powder.
Comparative example 6
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack Chinese medicine in the raw material of tilapia feed and lure Eat agent.
Comparative example 7
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare in the raw material of tilapia feed and lack dolphin chain Coccus disease biological agent.
Comparative example 8
Using Chinese patent application document " a kind of Tilapia mossambica high-yield and high-efficiency healthy aquaculture feed and preparation method thereof (application number: 201611082551.2) " technique of embodiment 1-3 prepares tilapia feed.
For the effect for verifying tilapia feed of the present invention, pass through following experimental verification.
Selection 100g or so, specification is almost consistent and makes a definite diagnosis Tilapia mossambica 330 for suffering from Streptococcus iniae disease, is randomly divided into 11 groups, i.e. test group 1, test group 2, test group 3, control group 1-8, every group Tilapia mossambica 30.The Tilapia mossambica of test group 1-3 is distinguished Feed the feed of 1-3 of the embodiment of the present invention.Control group Tilapia mossambica feeds with the feed of comparative example 1-8, continuous feeding 10 days, daily Feed intake 2 times, experimental period 10 days, after calculate Tilapia mossambica average growth rate and cure rate, as a result see the table below.
As seen from the above table: (1) by embodiment 1-3 and the comparative example 8(prior art), the data of comparative example 5-7 are as it can be seen that application Embodiment 1-3 tilapia feed, average growth rate, cure rate significantly improve；Simultaneously by the data of embodiment 1-3 as it can be seen that implementing Example 1 is optimum embodiment.
(2) by embodiment 1 and the data of comparative example 1-4 as it can be seen that additive I, additive II, additive III are in preparation Rofe Synergistic effect is played in fish meal, collaboration improves average growth rate, cure rate, this is:
Additive I contain thiocarbamic acid magnesium, potassium dimethyldithiocarbamate, aminoquinoxaline, cooperation additive II, Additive III uses, and can promote Tilapia mossambica and quickly absorbs the nutriment of feed, to improve the average growth rate of Tilapia mossambica, mentions High yield；Additive II contains potassium pyrophosphate, sodium stearate, sodium alginate, extremely strong dispersion performance is all had, containing hydrophilic End can make additive I, III reduce flocculated probability, promote additive I, III particle rapid dispersion in water, and promote pure worm Slurry protein powder, tapioca, corn flour, vitamin, 2- amino -4- carbamyl butyric acid, non-starch polysaccharide enzyme, microorganism system The substances rapid dispersions such as agent, Chinese medicine phagostimulant, Streptococcus iniae disease biological agent are to Tilapia mossambica enteron aisle, to promote Tilapia mossambica suction The effect of feed is received, Tilapia mossambica average growth rate, cure rate are improved, to improve yield；Additive III contains 2- amino -4- nitre Base sodium phenate, 5- nitroguaiacol sodium salt, additive III are added in tilapia feed, are used in compounding with additive I, rapidly into Enter in Tilapia mossambica body, can promote the appetite of Tilapia mossambica, Tilapia mossambica is promoted to give birth to the pure worm slurry protein powder in tilapia feed, dimension The component particles nutrient absorption such as element, 2- amino -4- carbamyl butyric acid, Chinese medicine phagostimulant, Streptococcus iniae disease biological agent, mentions Height suffers from the feed intake and the speed of growth of Streptococcus iniae disease Tilapia mossambica, enhances the immunity and anti-stress ability of Tilapia mossambica, reduces Tilapia mossambica suffers from the death rate of Streptococcus iniae disease, improves cure rate, finally improves yield.
The specific implementation of the invention is not to be limited to these illustrations for the above content, and technology belonging to the present invention is led For the those of ordinary skill in domain, without departing from the inventive concept of the premise, a number of simple deductions or replacements can also be made, It all shall be regarded as belonging to present invention scope of patent protection determined by the appended claims.

Claims (10)

1. a kind of Chinese medicine phagostimulant, which is characterized in that as unit of parts by weight, including following raw material: 8-12 parts of anise, 7-13 parts of Cortex Magnoliae Officinalis, 9-14 parts of radix pseudostellariae, 4-7 parts of peppermint, 5-10 parts of granatum, 2-5 parts of hawthorn, 3-6 parts of cortex cinnamomi, 2-6 parts of Radix Notoginseng, 4-12 parts of dried orange peel, 7-13 parts of glycine betaine, 1.2-1.8 parts of sweetener, 0.6-1 parts of flavouring agent, 0.5-0.9 parts of acid, pungent agent 1-1.6 parts, 0.7-1.2 parts of tasty agents.
2. Chinese medicine phagostimulant according to claim 1, which is characterized in that as unit of parts by weight, including following raw material: it is big 10 parts of fennel, 12 parts of Cortex Magnoliae Officinalis, 13 parts of radix pseudostellariae, 6 parts of peppermint, 9 parts of granatum, 4 parts of hawthorn, 5 parts of cortex cinnamomi, 5 parts of Radix Notoginseng, dried orange peel 10 Part, 12 parts of glycine betaine, 1.6 parts of sweetener, 0.9 part of flavouring agent, 0.7 part of acid, 1.2 parts of pungent agent, 0.8 part of tasty agents.
3. Chinese medicine phagostimulant according to claim 1 or 2, which is characterized in that the sweetener is cyclohexylsulfamic Sodium.
4. Chinese medicine phagostimulant according to claim 1 or 2, which is characterized in that the flavouring agent is ambrein.
5. Chinese medicine phagostimulant according to claim 1 or 2, which is characterized in that the acid is malic acid.
6. Chinese medicine phagostimulant according to claim 1 or 2, which is characterized in that the pungent agent is allicin.
7. Chinese medicine phagostimulant according to claim 1 or 2, which is characterized in that the tasty agents is Pidolidone.
8. a kind of application of Chinese medicine phagostimulant according to claim 1-7 in tilapia feed, feature exist In, the tilapia feed, as unit of parts by weight, including following raw material: 22-28 parts of pure worm slurry protein powder, Soybean Meal 12- 20 parts, 8-15 parts of tapioca, 6-10 parts of corn flour, 3-5 parts of Cottonseed Meal, 4-8 parts of rapeseed dregs, 1.5-2.8 parts of vitamin, 2- ammonia 1.2-2.5 parts of base -4- carbamyl butyric acid, 0.8-1.5 parts of non-starch polysaccharide enzyme, 2-3 parts of microorganism formulation, Chinese medicine phagostimulant 2.2-3 parts, 1-1.5 parts of Streptococcus iniae disease biological agent, I 0.9-1.2 parts of additive, II 1-1.8 parts of additive, additive III 0.6-0.9 parts.
9. application of the Chinese medicine phagostimulant according to claim 8 in tilapia feed, which is characterized in that the vitamin It as unit of parts by weight, is made of following raw material: raw 0.8 part of element A, 0.6 part of vitamin B1,0.4 part of vitamin C.
10. application of the Chinese medicine phagostimulant according to claim 8 in tilapia feed, which is characterized in that micro- life Object preparation is bacillus subtilis.
CN201811391494.5A 2018-11-21 2018-11-21 Chinese medicine phagostimulant and its application in tilapia feed Withdrawn CN109691588A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106721534A (en) * 2016-11-30 2017-05-31 广西南宁市武鸣明山红农业科技开发有限公司 A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease and preparation method thereof
CN107889950A (en) * 2017-11-27 2018-04-10 湖南七纬科技有限公司 A kind of creep mixed feed for piglets and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106721534A (en) * 2016-11-30 2017-05-31 广西南宁市武鸣明山红农业科技开发有限公司 A kind of feed for preventing and treating Tilapia mossambica Streptococcus iniae disease and preparation method thereof
CN107889950A (en) * 2017-11-27 2018-04-10 湖南七纬科技有限公司 A kind of creep mixed feed for piglets and preparation method thereof

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Application publication date: 20190430