CN106036205A - Feed additive capable of treating streptococcal disease of tilapia and preparation method of feed additive - Google Patents
Feed additive capable of treating streptococcal disease of tilapia and preparation method of feed additive Download PDFInfo
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- CN106036205A CN106036205A CN201610370626.0A CN201610370626A CN106036205A CN 106036205 A CN106036205 A CN 106036205A CN 201610370626 A CN201610370626 A CN 201610370626A CN 106036205 A CN106036205 A CN 106036205A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention belongs to the technical field of aquaculture, relates to a feed additive capable of treating a streptococcal disease of tilapia, and further relates to a preparation method of the feed additive capable of treating the streptococcal disease of the tilapia. The feed additive capable of treating the streptococcal disease of the tilapia disclosed by the invention is prepared from the following components in parts by mass: 33-42 parts of huguenot fern herb, 27-34 parts of Chinese honeylocust fruits, 25-32 parts of pomegranate rinds, 24-30 parts of wild mint, 22-29 parts of cortex fraxini, 21-28 parts of belvedere fruits, 18-25 parts of Chinese redbud bark, 18-24 parts of radix ophiopogonis, 17-24 parts of common ducksmeat herb, 16-22 parts of herb of ciliate bugle, 15-21 parts of lawn pennywort herb, 13-21 parts of common cnidium fruits, 13-20 parts of seeds of whiteflower cacalia, 12-17 parts of radix angelicae dahuricae, 10-18 parts of atalantia buxifolia roots and leaves, 9-15 parts of turmeric and the like. The feed additive disclosed by the invention can effectively treat the streptococcal disease of the tilapia.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of feedstuff treating tilapia streptococcicosis and add
Agent；The invention still further relates to the preparation method of the feed additive of a kind of above-mentioned treatment tilapia streptococcicosis.
Background technology
Tilapia is former is distributed in temperate zone, subtropical zone and torrid areas.Tilapia has become as the weight of World Water industry now
The cultured freshwater fish that some scientific research is cultivated, and one of it is described as the main source of following animal protein.Tilapia originates in
Africa, belongs to the tropic fishes of Family Cichlidae, similar with Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis), generally moves in fresh water, also can move in different salt content
Salt water in, can survive in lake, river, pond shallow water in, have the strongest adaptation ability, and the water that dissolved oxygen is less had
Extremely strong adaptability.Overwhelming majority tilapia is omnivorousness, often eats aquatic plant and minces.Tilapia is in the narrow and small waters of area
In also can breed.Even can grow in rice terrace.
Guangdong, Guangxi, Hainan San Sheng the most generally break out serious tilapia streptococcicosis, especially Maoming, height for 2009
Want, Huazhou, the ground such as Gaozhou county, cause serious economic loss to raiser.The tilapia of net cage and pond culture all has this disease
Occur, main harm parent fish and the juvenile fish of more than 100 grams and adult fish；The popular high-incidence season is 5～JIUYUE；Popular water temperature is 25 DEG C
～37 DEG C, easily send out for more than 32 DEG C in water temperature；Infectiousness is strong, and sickness rate reaches 20%～30%, and the mortality rate of morbidity fish is up to 80%
Above.Typically peel off on limit, pond solely trip, the health bending of ill tilapia is spinned, or is swum slowly on the water surface, bradykinesia,
Body colour is turned black, and the motion of fish body is unbalance, turbid white, eyeball evagination, the anus redness of cornea etc..Sick fish exophthalmos or muddiness are turned white, with
Time hemorrhage along with eye, under fish body buccal occur scale come off, the phenomenon such as hemorrhage, muscular death, other positions such as abdomeinal fin, breast
Fin, the base portion of tail fin have hemorrhage and with muscular death phenomenon；Gallbladder, liver, spleen enlargement, rotten to the corn time serious；Intestinal is sent out
Inflammation, the intestines and stomach is more empty, inside has hydrops or yellow mucus；Part ill fish internal organs such as intestinal, liver,spleen,kidney has bleeding.The most often
Therapeutic Method be 10% fluorine Ben Nikao (1 g/kg of feedstuff) for oral administration.But existing Therapeutic Method not only pollutes environment,
And therapeutic effect is limited.
Summary of the invention
An object of the present invention is to provide a kind of feed additive treating tilapia streptococcicosis, and its raw material components is equal
For green pure natural material, and can effectively treat tilapia streptococcicosis.
In order to solve above-mentioned technical problem, the present invention by the following technical solutions, a kind of tilapia streptococcicosis for the treatment of
Feed additive, is made up of each component of following masses number: Herba Pteridis Multifidae 33-42 part, Fructus Gleditsia 27-34 part, Pericarpium Granati 25-32
Part, wild mint 24-30 part, Cortex Fraxini 22-29 part, Fructus Kochiae 21-28 part, Cortex cercis chinensis 18-25 part, 18-24 part Radix Ophiopogonis, Herba Spirodelae 17-
24 parts, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 16-22 part, Herba Hydrocotyles 15-21 part, Fructus Cnidii 13-21 part, Semen Caesalpiniae Minacis 13-20 part, Radix Angelicae Dahuricae 12-17 part, east wind
Fructus Citri tangerinae 10-18 part, Rhizoma Curcumae Longae 9-15 part, Cortex Hibisci 8-16 part, RADIX PEUCEDANI 7-12 part, Herba Bidentis Bipinnatae 7-12 part, Rhizoma Bolbostematis 5-10 part, celestial being
Crane grass 4-6 part, euphausia superba powder 18-25 part, Fructus Crataegi 6-9 part, Entermorpha 12-20 part, water silk floss 16-20 part, fish oil 5-7 part.
Preferably, the feed additive of described treatment tilapia streptococcicosis, it is made up of each component of following masses number:
Herba Pteridis Multifidae 36 parts, 31 parts of Fructus Gleditsia, Pericarpium Granati 29 parts, wild mint 27 parts, Cortex Fraxini 26 parts, the Fructus Kochiae 24 parts, Cortex cercis chinensis 21 parts, Radix Ophiopogonis
21 parts, 20 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 18 parts, Herba Hydrocotyles 18 parts, Fructus Cnidii 17 parts, Semen Caesalpiniae Minacis 17 parts, the Radix Angelicae Dahuricae 15 parts, east wind Fructus Citri tangerinae 14
Part, 12 parts of Rhizoma Curcumae Longae, Cortex Hibisci 12 parts, RADIX PEUCEDANI 9 parts, Herba Bidentis Bipinnatae 9 parts, Rhizoma Bolbostematis 8 parts, Herba Agrimoniae 5 parts, euphausia superba powder 21
Part, Fructus Crataegi 8 parts, Entermorpha 15 parts, water continuous 18 parts, 6 parts of fish oil.
Preferably, the feed additive of described treatment tilapia streptococcicosis, it is made up of each component of following masses number:
Herba Pteridis Multifidae 38 parts, 30 parts of Fructus Gleditsia, Pericarpium Granati 27 parts, wild mint 27 parts, Cortex Fraxini 25 parts, the Fructus Kochiae 25 parts, Cortex cercis chinensis 22 parts, wheat
Winter 21 parts, 21 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 20 parts, Herba Hydrocotyles 18 parts, Fructus Cnidii 17 parts, Semen Caesalpiniae Minacis 16 parts, the Radix Angelicae Dahuricae 14 parts, east wind Fructus Citri tangerinae 15
Part, 12 parts of Rhizoma Curcumae Longae, Cortex Hibisci 12 parts, RADIX PEUCEDANI 10 parts, Herba Bidentis Bipinnatae 10 parts, Rhizoma Bolbostematis 7 parts, Herba Agrimoniae 5 parts, euphausia superba powder
22 parts, Fructus Crataegi 7 parts, Entermorpha 16 parts, water continuous 18 parts, 6 parts of fish oil.
In the present invention, Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, muscles and bones
Grass, Herba Hydrocotyles, Fructus Cnidii, Semen Caesalpiniae Minacis, the Radix Angelicae Dahuricae, east wind Fructus Citri tangerinae, Rhizoma Curcumae Longae, Cortex Hibisci, RADIX PEUCEDANI, Herba Bidentis Bipinnatae, Rhizoma Bolbostematis and red crowned crane
Grass with the combination of above-mentioned specific proportioning compounding together time, the pathogen streptococcus of tilapia streptococcicosis is had and significantly kills
Bacterium fungistatic effect.In the feed additive of the present invention, euphausia superba powder is nutritious, delicious flavour, also can improve tilapia
Immunity.Fructus Crataegi can appetite promoting and the spleen strengthening, help digestion the most stagnant, tilapia can be promoted to ingest more with the addition of the feedstuff of the present invention to add
The feedstuff of agent, and then the nutritional labeling in feedstuff can preferably be supplemented the nutrients for tilapia and muscle power, and make the present invention
Feed additive in the pathogen of tilapia streptococcicosis had the composition of notable bactericidal and bacteriostatic effect enter tilapia body
In and play its respective action.Entermorpha and water silk floss are the most with low cost, and nutritious, and Appropriate application Entermorpha and water silk floss can
Reduce environment and ecological pressure that Entermorpha and water silk floss bring, turn waste into wealth.And Entermorpha and water silk floss not only have food calling to tilapia
Effect, and containing abundant nutrition.Fish oil can be that tilapia supplements energy, and have food calling effect to tilapia.South
After pole krill meal, Fructus Crataegi, Entermorpha, the tender fish oil of water compound with above-mentioned special ratios, tilapia is had the effect of collaborative food calling,
Can improve the mouthfeel of feed additive, can ingest the feedstuff of the feed additive that more with the addition of the present invention by food calling tilapia,
And then in making nutritional labeling in feedstuff enter tilapia body and utilized by tilapia, also make in feed additive to tilapia
The pathogen of streptococcicosis has the composition of notable bactericidal and bacteriostatic effect and enters in tilapia body and play its respective action.
It is a further object of the present invention to provide the preparation side of the feed additive of a kind of above-mentioned treatment tilapia streptococcicosis
Method, the preparation method of the feed additive of this treatment tilapia streptococcicosis comprises the following steps:
(1) weigh Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, Radix Ophiopogonis according to proportioning, float
Duckweed, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis, addition quality be Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini,
The purified water of 12 times of skin, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis gross mass, 20-30
DEG C soak 24h, be then heated to boiling after boil 20 minutes, separate take out fried liquid；
(2) continuing to add quality in the medical material of step (1) is Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, Fructus Kochiae
The purified water of 8 times of son, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis gross mass, is heated to boiling
Continue after rising to boil 15 minutes, merge twice fried liquid and be extracting solution；
(3) Radix Angelicae Dahuricae, east wind Fructus Citri tangerinae, Rhizoma Curcumae Longae, Cortex Hibisci, RADIX PEUCEDANI, Herba Bidentis Bipinnatae, Rhizoma Bolbostematis and celestial being are weighed respectively according to proportioning
Crane grass, loads ball mill grinding, crosses 80 mesh sieves, obtains grounds travel；
(4) grounds travel that extracting solution step (2) obtained and step (3) obtain is positioned over the extraction in supercritical extraction instrument
Taking in still, utilize carbon dioxide as medium and extract, extraction kettle pressure is 40Mpa, and extraction temperature is 45 DEG C, separator pressure
Power 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid；
(5) euphausia superba powder, Fructus Crataegi, Entermorpha, the tender fish oil of water are weighed respectively according to proportioning, with the extraction of step (4) gained
Take liquid mix homogeneously, obtain object.
Usage and dosage: every 1kg fish body weight adds the feed additive of the 15-25mg present invention every time, mixes to raise and throws something and feeds, 1 day 2
Secondary, it is used in conjunction use 3-5 days.
In preparation method, by Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae,
Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis soak 24h in purified water, can make medical material deliquescing, are conducive in above-mentioned each component
Effective ingredient separate out in down-stream；By Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, wheat
Winter, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis decoct the irritation effect that can change above-mentioned each component, gentleer
Act on tilapia, promote the tilapia absorption to medicine.Grind the Radix Angelicae Dahuricae, east wind Fructus Citri tangerinae, Rhizoma Curcumae Longae, Cortex Hibisci, RADIX PEUCEDANI, ghost
The purpose of needle grass, Rhizoma Bolbostematis and Herba Agrimoniae is that the effective ingredient that can be beneficial in above-mentioned each component is analysed more fully at subsequent step
Go out.Additionally, the preparation method of the present invention is simple, and environment will not be brought pollution by preparation process.
The feed additive of the treatment tilapia streptococcicosis of the present invention, is the etiopathogenesis according to streptococcicosis, selects
Suitable component, and organically combined, there is significant cooperative synergism effect, played the merit of each component to greatest extent
Energy.The component of the feed additive of the treatment tilapia streptococcicosis of the present invention is environmental protection raw material, nothing nontoxic to environment
Evil, does not contains chemical drugs or antibiotic, does not interferes with the healthy of people after the edible tilapia cured, and this
Bright feed additive is good to the curative effect of tilapia streptococcicosis, and cure rate is high, makes the survival rate of ill tilapia
To more than 90%, after medication 2-4 days, tilapia starts progressively to recover to ingest, and ingestion frequency improves, and eyeball evagination, anus are red and swollen
Phenomenon disappears, and base portion bleeding is controlled, and scale obscission is controlled, and the ecchymosis of fish body surface progressively disappears, You Zi
Gradually recover normal.
Compared with prior art, the invention has the beneficial effects as follows:
(1) in the present invention, Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, muscle
GUCAO, Herba Hydrocotyles, Fructus Cnidii, Semen Caesalpiniae Minacis, the Radix Angelicae Dahuricae, east wind Fructus Citri tangerinae, Rhizoma Curcumae Longae, Cortex Hibisci, RADIX PEUCEDANI, Herba Bidentis Bipinnatae, Rhizoma Bolbostematis and celestial being
When crane grass compounds together with the combination of above-mentioned specific proportioning, the pathogen streptococcus of tilapia streptococcicosis is had significantly
Bactericidal and bacteriostatic effect.In the feed additive of the present invention, euphausia superba powder is nutritious, delicious flavour, also can improve tilapia
Immunity.Fructus Crataegi can appetite promoting and the spleen strengthening, help digestion the most stagnant, tilapia can be promoted to ingest more with the addition of the feedstuff of the present invention to add
Add the feedstuff of agent, and then the nutritional labeling in feedstuff can preferably be supplemented the nutrients for tilapia and muscle power, and make this
In bright feed additive, pathogen to tilapia streptococcicosis has the composition of notable bactericidal and bacteriostatic effect and enters tilapia
Internal and play its respective action.Entermorpha and water silk floss are the most with low cost, and nutritious, Appropriate application Entermorpha and water silk floss energy
Enough reduce environment and ecological pressure that Entermorpha and water silk floss bring, turn waste into wealth.And tilapia is not only had and lures by Entermorpha and water silk floss
Food effect, and containing abundant nutrition.Fish oil can be that tilapia supplements energy, and have food calling effect to tilapia.
After euphausia superba powder, Fructus Crataegi, Entermorpha, the tender fish oil of water compound with above-mentioned special ratios, tilapia is had the effect of collaborative food calling
Really, the mouthfeel of feed additive can be improved, can ingest and more with the addition of the raising of feed additive of the present invention by food calling tilapia
Material, and then in making nutritional labeling in feedstuff enter tilapia body and utilized by tilapia, also make in feed additive to sieve
The pathogen of non-fish streptococcicosis has the composition of notable bactericidal and bacteriostatic effect and enters in tilapia body and play its respective action.
(2), in preparation method, by Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, Radix Ophiopogonis, float
Duckweed, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis soak 24h in purified water, can make medical material deliquescing, are conducive to above-mentioned each group
Effective ingredient in Fen separates out in down-stream；By Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, cercis
Skin, Radix Ophiopogonis, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis decoct the irritation effect that can change above-mentioned each component,
Relatively mild act on tilapia, promote the tilapia absorption to medicine.Grind the Radix Angelicae Dahuricae, east wind Fructus Citri tangerinae, Rhizoma Curcumae Longae, Cortex Hibisci, pale reddish brown
The purpose of Radix Peucedani, Herba Bidentis Bipinnatae, Rhizoma Bolbostematis and Herba Agrimoniae is that the effective ingredient that can be beneficial in above-mentioned each component more fills at subsequent step
The precipitation divided.The preparation method of the present invention is simple, and environment will not be brought pollution by preparation process.
(3) feed additive of the treatment tilapia streptococcicosis of the present invention, is the etiopathogenesis according to streptococcicosis, choosing
The suitable component selected, and organically combined, there is significant cooperative synergism effect, played each component to greatest extent
Function.The component of the feed additive of the treatment tilapia streptococcicosis of the present invention is environmental protection raw material, nontoxic to environment
Harmless, do not contain chemical drugs or antibiotic, after the edible tilapia cured, do not interfere with the healthy of people, Er Qieben
The feed additive of invention is good to the curative effect of tilapia streptococcicosis, and cure rate is high, makes the survival rate of ill tilapia
Reaching more than 90%, after medication 2-4 days, tilapia starts progressively to recover to ingest, and ingestion frequency improves, and eyeball evagination, anus are red
Swollen phenomenon disappears, and base portion bleeding is controlled, and scale obscission is controlled, and the ecchymosis of fish body surface progressively disappears, trip
Appearance gradually recovers normal.
Detailed description of the invention
Below example facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiment
Method, if no special instructions, is conventional method.
Embodiment 1
A kind of feed additive treating tilapia streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae
35 parts, 27 parts of Fructus Gleditsia, Pericarpium Granati 29 parts, wild mint 24 parts, Cortex Fraxini 22 parts, the Fructus Kochiae 21 parts, Cortex cercis chinensis 20 parts, Radix Ophiopogonis 18 parts,
19 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 16 parts, Herba Hydrocotyles 15 parts, Fructus Cnidii 18 parts, Semen Caesalpiniae Minacis 13 parts, the Radix Angelicae Dahuricae 12 parts, east wind Fructus Citri tangerinae 15 parts, Rhizoma Curcumae Longae
9 parts, Cortex Hibisci 8 parts, RADIX PEUCEDANI 7 parts, Herba Bidentis Bipinnatae 7 parts, Rhizoma Bolbostematis 6 parts, Herba Agrimoniae 6 parts, euphausia superba powder 18 parts, Fructus Crataegi 6
Part, Entermorpha 20 parts, water continuous 16 parts, 6 parts of fish oil.
The preparation method of the feed additive of above-mentioned treatment tilapia streptococcicosis comprises the following steps:
(1) weigh Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, Radix Ophiopogonis according to proportioning, float
Duckweed, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis, addition quality is Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, Fructus Kochiae
The purified water of 12 times of son, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis gross mass, 20-30 DEG C
Soak 24h, boil 20 minutes after being then heated to boiling, separate and take out fried liquid；
(2) continuing to add quality in the medical material of step (1) is Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, Fructus Kochiae
The purified water of 8 times of son, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis gross mass, is heated to boiling
Continue after rising to boil 15 minutes, merge twice fried liquid and be extracting solution；
(3) Radix Angelicae Dahuricae, east wind Fructus Citri tangerinae, Rhizoma Curcumae Longae, Cortex Hibisci, RADIX PEUCEDANI, Herba Bidentis Bipinnatae, Rhizoma Bolbostematis and celestial being are weighed respectively according to proportioning
Crane grass, loads ball mill grinding, crosses 80 mesh sieves, obtains grounds travel；
(4) grounds travel that extracting solution step (2) obtained and step (3) obtain is positioned over the extraction in supercritical extraction instrument
Taking in still, utilize carbon dioxide as medium and extract, extraction kettle pressure is 40Mpa, and extraction temperature is 45 DEG C, separator
Pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid；
(5) euphausia superba powder, Fructus Crataegi, Entermorpha, the tender fish oil of water are weighed respectively according to proportioning, with the extraction of step (4) gained
Take liquid mix homogeneously, obtain object.
Embodiment 2
A kind of feed additive treating tilapia streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae
33 parts, 28 parts of Fructus Gleditsia, Pericarpium Granati 25 parts, wild mint 25 parts, Cortex Fraxini 22 parts, the Fructus Kochiae 21 parts, Cortex cercis chinensis 18 parts, Radix Ophiopogonis 20 parts,
17 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 16 parts, Herba Hydrocotyles 15 parts, Fructus Cnidii 13 parts, Semen Caesalpiniae Minacis 14 parts, the Radix Angelicae Dahuricae 17 parts, east wind Fructus Citri tangerinae 10 parts, Rhizoma Curcumae Longae
10 parts, Cortex Hibisci 9 parts, RADIX PEUCEDANI 7 parts, Herba Bidentis Bipinnatae 7 parts, Rhizoma Bolbostematis 5 parts, Herba Agrimoniae 4 parts, euphausia superba powder 20 parts, Fructus Crataegi 7
Part, Entermorpha 12 parts, water continuous 16 parts, 5 parts of fish oil.
The preparation method of the feed additive of the treatment tilapia streptococcicosis of the present embodiment is with embodiment 1.
Embodiment 3
A kind of feed additive treating tilapia streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae
42 parts, 33 parts of Fructus Gleditsia, Pericarpium Granati 32 parts, wild mint 30 parts, Cortex Fraxini 27 parts, the Fructus Kochiae 27 parts, Cortex cercis chinensis 25 parts, Radix Ophiopogonis 22 parts,
21 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 22 parts, Herba Hydrocotyles 21 parts, Fructus Cnidii 21 parts, Semen Caesalpiniae Minacis 20 parts, the Radix Angelicae Dahuricae 16 parts, east wind Fructus Citri tangerinae 18 parts, Rhizoma Curcumae Longae
15 parts, Cortex Hibisci 16 parts, RADIX PEUCEDANI 12 parts, Herba Bidentis Bipinnatae 12 parts, Rhizoma Bolbostematis 10 parts, Herba Agrimoniae 6 parts, euphausia superba powder 24 parts,
Fructus Crataegi 8 parts, Entermorpha 18 parts, water continuous 20 parts, 7 parts of fish oil.
The preparation method of the feed additive of the treatment tilapia streptococcicosis of the present embodiment is with embodiment 1.
Embodiment 4
A kind of feed additive treating tilapia streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae
40 parts, 34 parts of Fructus Gleditsia, Pericarpium Granati 30 parts, wild mint 27 parts, Cortex Fraxini 29 parts, the Fructus Kochiae 28 parts, Cortex cercis chinensis 25 parts, Radix Ophiopogonis 24 parts,
24 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 22 parts, Herba Hydrocotyles 21 parts, Fructus Cnidii 20 parts, Semen Caesalpiniae Minacis 20 parts, the Radix Angelicae Dahuricae 17 parts, east wind Fructus Citri tangerinae 17 parts, Rhizoma Curcumae Longae
14 parts, Cortex Hibisci 15 parts, RADIX PEUCEDANI 12 parts, Herba Bidentis Bipinnatae 12 parts, Rhizoma Bolbostematis 9 parts, Herba Agrimoniae 6 parts, euphausia superba powder 25 parts, mountain
Short, bristly hair or beard 9 parts, Entermorpha 20 parts, water continuous 19 parts, 7 parts of fish oil.
The preparation method of the feed additive of the treatment tilapia streptococcicosis of the present embodiment is with embodiment 1.
Embodiment 5
A kind of feed additive treating tilapia streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae
36 parts, 31 parts of Fructus Gleditsia, Pericarpium Granati 29 parts, wild mint 27 parts, Cortex Fraxini 26 parts, the Fructus Kochiae 24 parts, Cortex cercis chinensis 21 parts, Radix Ophiopogonis 21 parts,
20 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 18 parts, Herba Hydrocotyles 18 parts, Fructus Cnidii 17 parts, Semen Caesalpiniae Minacis 17 parts, the Radix Angelicae Dahuricae 15 parts, east wind Fructus Citri tangerinae 14 parts, Rhizoma Curcumae Longae
12 parts, Cortex Hibisci 12 parts, RADIX PEUCEDANI 9 parts, Herba Bidentis Bipinnatae 9 parts, Rhizoma Bolbostematis 8 parts, Herba Agrimoniae 5 parts, euphausia superba powder 21 parts, Fructus Crataegi
8 parts, Entermorpha 15 parts, water continuous 18 parts, 6 parts of fish oil.
The preparation method of the feed additive of the treatment tilapia streptococcicosis of the present embodiment is with embodiment 1.
Embodiment 6
A kind of feed additive treating tilapia streptococcicosis, is made up of each component of following masses number: preferably,
The feed additive of described treatment tilapia streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae 38 parts, Fructus Gleditsia
30 parts, Pericarpium Granati 27 parts, wild mint 27 parts, Cortex Fraxini 25 parts, the Fructus Kochiae 25 parts, Cortex cercis chinensis 22 parts, Radix Ophiopogonis 21 parts, 21 parts of Herba Spirodelae,
Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 20 parts, Herba Hydrocotyles 18 parts, Fructus Cnidii 17 parts, Semen Caesalpiniae Minacis 16 parts, the Radix Angelicae Dahuricae 14 parts, east wind Fructus Citri tangerinae 15 parts, 12 parts of Rhizoma Curcumae Longae, Hibiscus syriacus L.
Skin 12 parts, RADIX PEUCEDANI 10 parts, Herba Bidentis Bipinnatae 10 parts, Rhizoma Bolbostematis 7 parts, Herba Agrimoniae 5 parts, euphausia superba powder 22 parts, Fructus Crataegi 7 parts, waterside
Tongue 16 parts, water continuous 18 parts, 6 parts of fish oil.
The preparation method of the feed additive of the treatment tilapia streptococcicosis of the present embodiment is with embodiment 1.
Experimental result
Contrast experiment 1
Test site is selected in fishing ground, Qingdao, in order to verify the therapeutic effect of the present invention, to sieve suffering from streptococcicosis
Non-fish is tested:
The process of matched group is as follows: mixes and raises the 10% fluorine Ben Nikao that throws something and feeds (1 g/kg of feedstuff), every twice-daily, until sick fish
Recovery from illness.
The process of experiment A group is as follows: every 1kg fish body weight adds the feed additive of the 20mg embodiment of the present invention 1 every time, mixes
Raise and throw something and feed, 1 day 2 times, until sick fish recovery from illness.
The process of experiment B group is as follows: every 1kg fish body weight adds the feed additive of the 20mg embodiment of the present invention 2 every time, mixes
Raise and throw something and feed, 1 day 2 times, until sick fish recovery from illness.
The process of experiment C group is as follows: every 1kg fish body weight adds the feed additive of the 20mg embodiment of the present invention 3 every time, mixes
Raise and throw something and feed, 1 day 2 times, until sick fish recovery from illness.
The process of experiment D group is as follows: every 1kg fish body weight adds the feed additive of the 20mg embodiment of the present invention 4 every time, mixes
Raise and throw something and feed, 1 day 2 times, until sick fish recovery from illness.
The process of experiment E group is as follows: every 1kg fish body weight adds the feed additive of the 20mg embodiment of the present invention 5 every time, mixes
Raise and throw something and feed, 1 day 2 times, until sick fish recovery from illness.
The process of experiment F group is as follows: every 1kg fish body weight adds the feed additive of the 20mg embodiment of the present invention 6 every time, mixes
Raise and throw something and feed, 1 day 2 times, until sick fish recovery from illness.
Wherein, the standard of sick fish recovery from illness is: tilapia starts progressively to recover to ingest, and ingestion frequency improves, eyeball evagination, anus
The red and swollen phenomenon of door disappears, and base portion bleeding is controlled, and scale obscission is controlled, and the ecchymosis of fish body surface disappears, trip
Appearance recovers normal.
Experimental result is shown in Table 1:
The feed additive therapeutic effect of the treatment tilapia streptococcicosis of table 1 present invention
Test result indicate that: the feed additive of the present invention is good to the curative effect of tilapia streptococcicosis, cure rate is high,
Making the survival rate of ill tilapia to more than 90%, after medication 2-4 days, tilapia starts progressively to recover to ingest, frequency of ingesting
Rate improves, and eyeball evagination, anus redness phenomenon disappear, and base portion bleeding is effectively controlled, and scale obscission is controlled
System, the ecchymosis of fish body surface progressively disappears, and trip appearance gradually recovers normal.
The above is only the preferred embodiment of the present invention, it should be pointed out that: for the ordinary skill people of the art
For Yuan, under the premise without departing from the principles of the invention, it is also possible to make some improvement, these improve the guarantor that should be regarded as the present invention
Protect scope.
Claims (4)
1. the feed additive treating tilapia streptococcicosis, it is characterised in that: by each component group of following masses number
Become: Herba Pteridis Multifidae 33-42 part, Fructus Gleditsia 27-34 part, Pericarpium Granati 25-32 part, wild mint 24-30 part, Cortex Fraxini 22-29 part, the Fructus Kochiae
21-28 part, Cortex cercis chinensis 18-25 part, 18-24 part Radix Ophiopogonis, Herba Spirodelae 17-24 part, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 16-22 part, Herba Hydrocotyles 15-21 part, Serpentis
Machine tool 13-21 part, Semen Caesalpiniae Minacis 13-20 part, Radix Angelicae Dahuricae 12-17 part, east wind Fructus Citri tangerinae 10-18 part, Rhizoma Curcumae Longae 9-15 part, Cortex Hibisci 8-16 part,
RADIX PEUCEDANI 7-12 part, Herba Bidentis Bipinnatae 7-12 part, Rhizoma Bolbostematis 5-10 part, Herba Agrimoniae 4-6 part, euphausia superba powder 18-25 part, Fructus Crataegi 6-
9 parts, Entermorpha 12-20 part, water silk floss 16-20 part, fish oil 5-7 part.
The feed additive for the treatment of tilapia streptococcicosis the most according to claim 1, it is characterised in that: described treatment sieve
The feed additive of non-fish streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae 36 parts, 31 parts of Fructus Gleditsia, Punica granatum L.
Skin 29 parts, wild mint 27 parts, Cortex Fraxini 26 parts, the Fructus Kochiae 24 parts, Cortex cercis chinensis 21 parts, Radix Ophiopogonis 21 parts, 20 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 18
Part, Herba Hydrocotyles 18 parts, Fructus Cnidii 17 parts, Semen Caesalpiniae Minacis 17 parts, the Radix Angelicae Dahuricae 15 parts, east wind Fructus Citri tangerinae 14 parts, 12 parts of Rhizoma Curcumae Longae, Cortex Hibisci 12 parts,
RADIX PEUCEDANI 9 parts, Herba Bidentis Bipinnatae 9 parts, Rhizoma Bolbostematis 8 parts, Herba Agrimoniae 5 parts, euphausia superba powder 21 parts, Fructus Crataegi 8 parts, Entermorpha 15 parts, water
Continuous 18 parts, 6 parts of fish oil.
The feed additive for the treatment of tilapia streptococcicosis the most according to claim 1, it is characterised in that: described treatment sieve
The feed additive of non-fish streptococcicosis, is made up of each component of following masses number: Herba Pteridis Multifidae 38 parts, 30 parts of Fructus Gleditsia, Punica granatum L.
Skin 27 parts, wild mint 27 parts, Cortex Fraxini 25 parts, the Fructus Kochiae 25 parts, Cortex cercis chinensis 22 parts, Radix Ophiopogonis 21 parts, 21 parts of Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.) 20
Part, Herba Hydrocotyles 18 parts, Fructus Cnidii 17 parts, Semen Caesalpiniae Minacis 16 parts, the Radix Angelicae Dahuricae 14 parts, east wind Fructus Citri tangerinae 15 parts, 12 parts of Rhizoma Curcumae Longae, Cortex Hibisci 12 parts,
RADIX PEUCEDANI 10 parts, Herba Bidentis Bipinnatae 10 parts, Rhizoma Bolbostematis 7 parts, Herba Agrimoniae 5 parts, euphausia superba powder 22 parts, Fructus Crataegi 7 parts, Entermorpha 16 parts,
Water continuous 18 parts, 6 parts of fish oil.
4. a preparation method for the feed additive of the treatment tilapia streptococcicosis according to any one of claim 1-3,
It is characterized in that: comprise the following steps:
(1) Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, muscle are weighed according to proportioning
GUCAO, Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis, addition quality is Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae, purple
The purified water of 12 times of chaste tree skin, Radix Ophiopogonis, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis gross mass, 20-30 DEG C of immersion
24h, boils 20 minutes after being then heated to boiling, separates and takes out fried liquid；
(2) continue in the medical material of step (1) add quality be Herba Pteridis Multifidae, Fructus Gleditsia, Pericarpium Granati, wild mint, Cortex Fraxini, the Fructus Kochiae,
The purified water of 8 times of Cortex cercis chinensis, Radix Ophiopogonis, Herba Spirodelae, Herba ajugae ciliatae (Herba Ajugae Ajuga ciliata Bge.), Herba Hydrocotyles, Fructus Cnidii and Semen Caesalpiniae Minacis gross mass, after being heated to boiling
Continue to boil 15 minutes, merge twice fried liquid and be extracting solution；
(3) Radix Angelicae Dahuricae, east wind Fructus Citri tangerinae, Rhizoma Curcumae Longae, Cortex Hibisci, RADIX PEUCEDANI, Herba Bidentis Bipinnatae, Rhizoma Bolbostematis and red crowned crane are weighed respectively according to proportioning
Grass, loads ball mill grinding, crosses 80 mesh sieves, obtains grounds travel；
(4) grounds travel that extracting solution step (2) obtained and step (3) obtain is positioned over the extraction kettle in supercritical extraction instrument
In, utilizing carbon dioxide as medium and extract, extraction kettle pressure is 40Mpa, and extraction temperature is 45 DEG C, separator pressure
12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid；
(5) euphausia superba powder, Fructus Crataegi, Entermorpha, the tender fish oil of water are weighed respectively according to proportioning, with the extract of step (4) gained
Mix homogeneously, obtains object.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106376779A (en) * | 2016-10-28 | 2017-02-08 | 青岛海之星生物科技有限公司 | Feed additive for treating vibrio diseases of large yellow croakers and preparation method of feed additive |
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| CN101971928A (en) * | 2010-10-15 | 2011-02-16 | 广州市番禺区大川饲料有限公司 | Artificial compound feed with function of preventing tilapia mossambica streptococcicosis |
| CN103392940A (en) * | 2013-08-06 | 2013-11-20 | 中国水产科学研究院淡水渔业研究中心 | Feed for improving streptococcal disease resistance of tilapia and feeding method thereof |
| CN103844143A (en) * | 2014-03-11 | 2014-06-11 | 广西大学 | Chinese herbal compound feed additive preventing streptococcicosis for tilapia mossambica |
| CN105211534A (en) * | 2015-09-29 | 2016-01-06 | 广东恒兴饲料实业股份有限公司 | A kind ofly prevent mixed feed of Tilapia mossambica streptococcosis and preparation method thereof |
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| CN101971928A (en) * | 2010-10-15 | 2011-02-16 | 广州市番禺区大川饲料有限公司 | Artificial compound feed with function of preventing tilapia mossambica streptococcicosis |
| CN103392940A (en) * | 2013-08-06 | 2013-11-20 | 中国水产科学研究院淡水渔业研究中心 | Feed for improving streptococcal disease resistance of tilapia and feeding method thereof |
| CN103844143A (en) * | 2014-03-11 | 2014-06-11 | 广西大学 | Chinese herbal compound feed additive preventing streptococcicosis for tilapia mossambica |
| CN105211534A (en) * | 2015-09-29 | 2016-01-06 | 广东恒兴饲料实业股份有限公司 | A kind ofly prevent mixed feed of Tilapia mossambica streptococcosis and preparation method thereof |
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