CN111513209A - TiLV (TiLV-resistant Tilapia mossambica) feed additive and application - Google Patents
TiLV (TiLV-resistant Tilapia mossambica) feed additive and application Download PDFInfo
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- CN111513209A CN111513209A CN202010365161.6A CN202010365161A CN111513209A CN 111513209 A CN111513209 A CN 111513209A CN 202010365161 A CN202010365161 A CN 202010365161A CN 111513209 A CN111513209 A CN 111513209A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a TiLV feed additive for resisting tilapia and application thereof, relating to the technical field of feed additives, and the key points of the technical scheme are as follows: the feed additive comprises the following raw materials in parts by weight: 20-30 parts of dandelion and honeysuckle respectively, 15-20 parts of oriental wormwood, rheum officinale and angelica sinensis respectively, 7-10 parts of plantain seed and scutellaria baicalensis respectively, 10-15 parts of liquorice, 5-8 parts of aloe, 12-18 parts of dried orange peel and 50-80 parts of a carrier; the immunity of tilapia to the lake Luo virus can be enhanced, the tilapia is economical, environment-friendly and non-toxic, and the breeding system is continuously disinfected, so that the virus infection probability of tilapia is effectively reduced, and the survival rate of tilapia infected with the lake Luo virus is improved.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a TiLV (TiLV-resistant Tilapia mossambica) feed additive and application thereof.
Background
Tilapia mossambica is one of important economic fish species for freshwater aquaculture in the world, can be cultured in high density, and is an important source of animal protein. Tilapia is originally Africa and belongs to tropical fishes, and the tilapia comprises more than 100 subspecies. Tilapia has the characteristics of fast generation, high yield, food impurities, few diseases, strong fecundity and the like. At present, the tilapia cultivation in China is mainly concentrated in tropical and subtropical areas such as Guangdong, Guangxi, Hainan and the like, and mainly comprises pond intensive cultivation. Except for domestic marketing, some enterprises use tilapia to make fillets, fish steaks and the like, and export foreign countries, and the tilapia is an excellent breeding variety.
Compared with other fishes, tilapia is once famous for easy cultivation and strong disease resistance. However, in recent years, the breeding related to virus and the mass death of wild tilapia are sequentially outbreaked in Israel, Ecuador, Egyptian and other countries, which causes great loss to the tilapia breeding industry. Through research, the pathogens causing the massive death of Tilapia are all lake Luo virus (Tilapia lakevirus, TiLV), which is a negative strand RNA virus, and the genome consists of 10 independent gene segments. Currently, the tenth viral classification report of the international committee for viral classification (ICTV) has listed TiLV as a new species individually for tilapia mossamvirus species and new genus of tilapia mossamvirus.
The Luo lake virus can grow at 24-33 ℃, the optimal growth temperature is 25 ℃, and the lethality rate exceeds 70%. In 2017, in 5 months, the virus is detected from the domestic cultivated tilapia for the first time. However, few studies are currently made on how to prevent and treat the Luo lake virus disease in the tilapia mossambica breeding process. And because the Rou lake virus is infected, the disease is fast to occur and the death rate is high, great loss is caused to the tilapia culture. Therefore, how to research and design a feed additive for resisting TiLV of tilapia is a problem which is urgently needed to be solved at present.
Disclosure of Invention
The invention aims to provide a TiLV feed additive for tilapia and application thereof, which can enhance the immunity of tilapia to lake ro virus, are economic, environment-friendly and non-toxic, and can be used for continuously disinfecting a culture system, so that the virus infection probability of tilapia is effectively reduced, and the survival rate of tilapia infected with lake ro virus is improved.
The technical purpose of the invention is realized by the following technical scheme: a TiLV feed additive for tilapia resistance and an application thereof are provided, wherein the feed additive is composed of the following raw materials in parts by weight: 20-30 parts of dandelion and honeysuckle respectively, 15-20 parts of oriental wormwood, rhubarb and angelica respectively, 7-10 parts of plantain seed and scutellaria respectively, 10-15 parts of liquorice, 5-8 parts of aloe, 12-18 parts of dried orange peel and 50-80 parts of carrier.
Preferably, the feed additive consists of the following raw materials in parts by weight: 20 parts of dandelion, 20 parts of honeysuckle, 15 parts of oriental wormwood, 15 parts of rheum officinale, 15 parts of angelica sinensis, 10 parts of plantain seed, 10 parts of scutellaria baicalensis, 15 parts of liquorice, 8 parts of aloe, 12 parts of dried orange peel and 60 parts of a carrier.
Preferably, the feed additive consists of the following raw materials in parts by weight: 25 parts of dandelion, 25 parts of honeysuckle, 16 parts of oriental wormwood, 16 parts of rheum officinale, 16 parts of angelica sinensis, 8 parts of plantain seed, 8 parts of scutellaria baicalensis, 12 parts of liquorice, 6 parts of aloe, 16 parts of dried orange peel and 60 parts of a carrier.
Preferably, the feed additive also comprises 1-2 parts of hydrogen peroxide.
Preferably, the carrier is one or a combination of more than two of fish meal, corn meal, flour, bean pulp, rapeseed meal and rice bran.
Preferably, the feed additive is a granulated granule with the size of 60-80 meshes.
Preferably, the feed additive has a water content of 5-6%.
Preferably, the weight ratio of the feed additive to the feed is 2-3: 200.
In conclusion, the invention has the following beneficial effects:
1. the immunity and disease resistance of tilapia to lake luo virus can be enhanced, and the tilapia is economical, environment-friendly and non-toxic;
2. by continuously eating the feed containing the feed additive, the probability of virus infection of tilapia is effectively reduced, and the survival rate of tilapia infected with lake ro virus is improved;
3. the culture system is continuously sterilized, and the way of horizontal infection of tilapia lake Rou virus is effectively cut off.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more apparent, the present invention is further described in detail below with reference to the following embodiments.
Example 1
A TiLV feed additive for resisting tilapia and an application thereof are disclosed, wherein the feed additive is composed of the following raw materials in parts by weight: 30 parts of dandelion, 20 parts of honeysuckle, 20 parts of oriental wormwood, 15 parts of rheum officinale, 20 parts of angelica sinensis, 7 parts of plantain seed, 10 parts of scutellaria baicalensis, 10 parts of liquorice, 8 parts of aloe, 12 parts of dried orange peel and 80 parts of carrier. The carrier is fish meal.
The preparation method and the application thereof are as follows: (1) weighing the traditional Chinese medicines and the carrier according to the parts by weight; (2) pulverizing Chinese medicinal materials, adding carrier, stirring, and mixing for 20min to obtain Chinese medicinal powder; (3) preparing the mixed traditional Chinese medicine powder into granulated particles with the size of 60 meshes by a granulator; (4) controlling the water content of the feed additive to be 6%; (5) the weight ratio of the feed additive to the feed is 1: 100.
Example 2
A TiLV feed additive for resisting tilapia and an application thereof are disclosed, wherein the feed additive is composed of the following raw materials in parts by weight: 20 parts of dandelion, 30 parts of honeysuckle, 15 parts of oriental wormwood, 20 parts of rheum officinale, 15 parts of angelica sinensis, 10 parts of plantain seed, 7 parts of scutellaria baicalensis, 15 parts of liquorice, 5 parts of aloe, 18 parts of dried orange peel and 50 parts of carrier. The carrier is corn flour.
The preparation method and the application thereof are as follows: (1) weighing the traditional Chinese medicines and the carrier according to the parts by weight; (2) pulverizing Chinese medicinal materials, adding carrier, stirring, and mixing for 20min to obtain Chinese medicinal powder; (3) preparing the mixed traditional Chinese medicine powder into granulated particles with the size of 80 meshes by a granulator; (4) controlling the water content of the feed additive to be 5%; (5) the weight ratio of the feed additive to the feed is 3: 200.
Example 3
A TiLV feed additive for resisting tilapia and an application thereof are disclosed, wherein the feed additive is composed of the following raw materials in parts by weight: 20 parts of dandelion, 20 parts of honeysuckle, 15 parts of oriental wormwood, 15 parts of rheum officinale, 15 parts of angelica sinensis, 10 parts of plantain seed, 10 parts of scutellaria baicalensis, 15 parts of liquorice, 8 parts of aloe, 12 parts of dried orange peel and 60 parts of a carrier. The carrier is flour.
The preparation method and the application thereof are as follows: (1) weighing the traditional Chinese medicines and the carrier according to the parts by weight; (2) pulverizing Chinese medicinal materials, adding carrier, stirring, and mixing for 20min to obtain Chinese medicinal powder; (3) preparing the mixed traditional Chinese medicine powder into granulated particles with the size of 60 meshes by a granulator; (4) controlling the water content of the feed additive to be 6%; (5) the weight ratio of the feed additive to the feed is 1: 100.
Example 4
A TiLV feed additive for resisting tilapia and an application thereof are disclosed, wherein the feed additive is composed of the following raw materials in parts by weight: 25 parts of dandelion, 25 parts of honeysuckle, 16 parts of oriental wormwood, 16 parts of rheum officinale, 16 parts of angelica sinensis, 8 parts of plantain seed, 8 parts of scutellaria baicalensis, 12 parts of liquorice, 6 parts of aloe, 16 parts of dried orange peel and 60 parts of a carrier. The carrier is soybean meal.
The preparation method and the application thereof are as follows: (1) weighing the traditional Chinese medicines and the carrier according to the parts by weight; (2) pulverizing Chinese medicinal materials, adding carrier, stirring, and mixing for 20min to obtain Chinese medicinal powder; (3) preparing the mixed traditional Chinese medicine powder into granulated particles with the size of 80 meshes by a granulator; (4) controlling the water content of the feed additive to be 5%; (5) the weight ratio of the feed additive to the feed is 3: 200.
Example 5
A TiLV feed additive for resisting tilapia and an application thereof are disclosed, wherein the feed additive is composed of the following raw materials in parts by weight: 20 parts of dandelion, 20 parts of honeysuckle, 15 parts of oriental wormwood, 15 parts of rheum officinale, 15 parts of angelica sinensis, 10 parts of plantain seed, 10 parts of scutellaria baicalensis, 15 parts of liquorice, 8 parts of aloe, 12 parts of dried orange peel, 1 part of hydrogen peroxide and 60 parts of a carrier. The carrier is rapeseed meal.
The preparation method and the application thereof are as follows: (1) weighing the traditional Chinese medicines and the carrier according to the parts by weight; (2) pulverizing Chinese medicinal materials, adding carrier, stirring, and mixing for 20min to obtain Chinese medicinal powder; (3) preparing the mixed traditional Chinese medicine powder into granulated particles with the size of 60 meshes by a granulator; (4) controlling the water content of the feed additive to be 6%; (5) the weight ratio of the feed additive to the feed is 1: 100.
Example 6
A TiLV feed additive for resisting tilapia and an application thereof are disclosed, wherein the feed additive is composed of the following raw materials in parts by weight: 25 parts of dandelion, 25 parts of honeysuckle, 16 parts of oriental wormwood, 16 parts of rheum officinale, 16 parts of angelica sinensis, 8 parts of plantain seed, 8 parts of scutellaria baicalensis, 12 parts of liquorice, 6 parts of aloe, 16 parts of dried orange peel, 2 parts of hydrogen peroxide and 60 parts of a carrier. The carrier is rice bran.
The preparation method and the application thereof are as follows: (1) weighing the traditional Chinese medicines and the carrier according to the parts by weight; (2) pulverizing Chinese medicinal materials, adding carrier, stirring, and mixing for 20min to obtain Chinese medicinal powder; (3) preparing the mixed traditional Chinese medicine powder into granulated particles with the size of 80 meshes by a granulator; (4) controlling the water content of the feed additive to be 5%; (5) the weight ratio of the feed additive to the feed is 3: 200.
Control group: feeding with common feed.
Experimental results and analysis: selecting 7 5000L circular plastic barrels for internal circulation fish culture, wherein the number of the plastic barrels is A, B, C, D, E, F, G respectively, breeding 200 nile tilapia (the body length is 6.3 +/-1.6 cm) in each plastic barrel, and feeding the healthy tilapia with the feed mixed with the feed additive in the above examples 1-6 and the feed in the control group; after 7 days, the tilapia mossambica in the examples 1-6 is soaked in the suspension with the TiLV for 2 hours, and then the tilapia mossambica is put back into a plastic bucket to continue feeding; randomly picking 20 groupers on days 3 and 7, extracting RNA samples, performing reverse transcription on the RNA samples to obtain cDNA, and detecting the cDNA by using a TiLV primer (Tattiyapong et al, J Fish Dis.2018); and counting the TiLV infection rate of the tilapia at 3 days, the TiLV infection rate of the tilapia at 7 days and the TiLV death rate of the tilapia at 10 days. Among them, the mortality rate at day 10 is the proportion of deaths in the infected population. The results are shown in table 1:
TABLE 1 Tilapia infection in Luo lake Virus (TiLV) results statistics
Experimental analysis: as can be seen from the data in the table, the feed additives in examples 1-6 can enhance the immunity and disease resistance of tilapia to lake ro virus, effectively reduce the probability of tilapia virus infection on days 3 and 7, and improve the survival rate of tilapia infected with lake ro virus on day 10. Among them, the survival rate was the highest, reaching 50% in examples 2 and 5. The method is characterized in that traditional Chinese medicine components are added to a tilapia culture system, so that the way of horizontal infection of tilapia lake Rou virus is effectively cut off.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (8)
1. The TiLV feed additive for tilapia resistance and the application thereof are characterized in that the feed additive is composed of the following raw materials in parts by weight: 20-30 parts of dandelion and honeysuckle respectively, 15-20 parts of oriental wormwood, rhubarb and angelica respectively, 7-10 parts of plantain seed and scutellaria respectively, 10-15 parts of liquorice, 5-8 parts of aloe, 12-18 parts of dried orange peel and 50-80 parts of carrier.
2. The feed additive for resisting TiLV of tilapia according to claim 1 and the application thereof, wherein the feed additive is composed of the following raw materials by weight: 20 parts of dandelion, 20 parts of honeysuckle, 15 parts of oriental wormwood, 15 parts of rheum officinale, 15 parts of angelica sinensis, 10 parts of plantain seed, 10 parts of scutellaria baicalensis, 15 parts of liquorice, 8 parts of aloe, 12 parts of dried orange peel and 60 parts of a carrier.
3. The feed additive for resisting TiLV of tilapia according to claim 1 and the application thereof, wherein the feed additive is composed of the following raw materials by weight: 25 parts of dandelion, 25 parts of honeysuckle, 16 parts of oriental wormwood, 16 parts of rheum officinale, 16 parts of angelica sinensis, 8 parts of plantain seed, 8 parts of scutellaria baicalensis, 12 parts of liquorice, 6 parts of aloe, 16 parts of dried orange peel and 60 parts of a carrier.
4. The feed additive for resisting TiLV of tilapia according to claim 1, wherein said feed additive further comprises 1-2 parts of hydrogen peroxide.
5. The feed additive for resisting TiLV of tilapia according to claim 1, wherein said carrier is one or more of fish meal, corn meal, flour, bean pulp, rapeseed meal and rice bran.
6. The feed additive for resisting TiLV of tilapia according to claim 1, wherein said feed additive is granulated particles with a size of 60-80 meshes.
7. The feed additive for resisting TiLV of tilapia mossambica as claimed in claim 1, wherein the water content of said feed additive is 5-6%.
8. The feed additive for resisting TiLV of tilapia according to any one of claims 1-7 and application thereof, wherein the weight ratio of the feed additive to the feed is 2-3: 200.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010365161.6A CN111513209A (en) | 2020-04-30 | 2020-04-30 | TiLV (TiLV-resistant Tilapia mossambica) feed additive and application |
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| CN202010365161.6A CN111513209A (en) | 2020-04-30 | 2020-04-30 | TiLV (TiLV-resistant Tilapia mossambica) feed additive and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112056465A (en) * | 2020-10-16 | 2020-12-11 | 中国水产科学研究院淡水渔业研究中心 | Tilapia feed additive, tilapia feed and tilapia feeding method |
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| CN107349385A (en) * | 2017-07-19 | 2017-11-17 | 济南金牧生物科技有限公司 | One group is used for fermented tcm composition that live pig nonreactive cultivates and preparation method thereof |
Non-Patent Citations (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112056465A (en) * | 2020-10-16 | 2020-12-11 | 中国水产科学研究院淡水渔业研究中心 | Tilapia feed additive, tilapia feed and tilapia feeding method |
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Application publication date: 20200811 |