CN105661556A - Herba cistanche dietary fibers and preparation method and application thereof - Google Patents
Herba cistanche dietary fibers and preparation method and application thereof Download PDFInfo
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- CN105661556A CN105661556A CN201610079178.9A CN201610079178A CN105661556A CN 105661556 A CN105661556 A CN 105661556A CN 201610079178 A CN201610079178 A CN 201610079178A CN 105661556 A CN105661556 A CN 105661556A
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 136
- 238000002360 preparation method Methods 0.000 title claims abstract description 58
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- OFFIBSQEPVXQQW-UHFFFAOYSA-N formic acid;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound OC=O.OC(=O)CC(O)(C(O)=O)CC(O)=O OFFIBSQEPVXQQW-UHFFFAOYSA-N 0.000 description 1
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- 229940096919 glycogen Drugs 0.000 description 1
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- 201000002313 intestinal cancer Diseases 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides herba cistanche dietary fibers and a preparation method and application thereof. The preparation method includes the following steps that 1, herba cistanche residues and an extracting solution are mixed, supernatant and sediment are obtained through extraction and solid-liquid separation, the sediment is dried, and insoluble dietary fibers are obtained, wherein the extracting solution is any one of acid liquor, alkali liquor and enzyme liquor; 2, the supernatant or concentrated supernatant and absolute ethyl alcohol are mixed for alcohol precipitation, floccule is obtained and dried, and soluble dietary fibers are obtained. The preparation method of the herba cistanche dietary fibers is simple, the soluble dietary fibers and the insoluble dietary fibers can be obtained from the herba cistanche residues, comprehensive utilization of herba cistanche is achieved, and industrial production and application are convenient. The herba cistanche dietary fibers prepared through the preparation method are high in purity and good in color and luster, has excellent performance of relaxing bowels and restraining the activity of alpha-glucosidase, and is widely applied to the field of healthcare food.
Description
Technical field
The invention belongs to technical field of bioengineering, relate to a kind of Herba Cistanches dietary fiber, its preparation method and application, particularly relate to a kind of method extracting Herba Cistanches dietary fiber from Herba Cistanches residue and application thereof.
Background technology
Dietary fiber (dietaryfiber, DF) refers to can not be digested and assimilated by human small intestine, to human body unsoundness meaning, the carbohydrate of the degree of polymerization >=3 and lignin, including cellulose, hemicellulose and pectin etc. Dietary fiber has prevention cardiovascular disease, obesity, diabetes, hypertension, intestinal flora function of improving, reduces gastric and intestinal cancer generation, removes the physiological effecies such as external source harmful substance, antioxidation and scavenging free radicals, is referred to as " the 7th class nutrient " after saccharide, protein, fat, vitamin, mineral and water.
Water soluble dietary fiber and insoluble dietary fiber can be divided into by dietary fiber dissolubility. Water soluble dietary fiber refers mainly to the stored substance in cell wall and secretions, such as pectin, part hemicellulose and plant gum etc.; Insoluble dietary fiber refers mainly to the ingredient of cell wall, such as cellulose, part hemicellulose, lignin, chitosan and vegetable wax etc. At present, the method extracting dietary fiber both at home and abroad mainly has chemical method, enzyme process, chemistry-enzyme combined techniques, membrane separation process and fermentation method. Chemical method produces the three wastes in process of production, and environmental pollution is serious, and embrane method is subject to the restriction of working condition, not easily realizes industrialized production at present, and the advantage of enzyme method technique is in that not need High Temperature High Pressure, saves the energy, easy to operate, is beneficial to environmental conservation.
Herba Cistanches has another name called gold Radix Crotalariae szemoensis, Herba Stellariae Saxatilis, Herba Cistanches or big rue, for the fleshy stem of the dry zone scale leaf of orobanchaceae plant cistanche, main product in the Inner Mongol, Gansu, the ground such as Xinjiang and Qinghai;Modern pharmacological research shows, Herba Cistanches has many-sided effects such as raising sexual function, defying age, raising ability of learning and memory, anti-senile dementia disease, relieving constipation, has the good reputation of " desert Radix Ginseng ". At present, the main active of Herba Cistanches is polysaccharide and phenethyl alcohol glycoside compounds, and the residue availability having extracted polysaccharide and phenethyl alcohol glycoside is very low, and this residue accounts for the 30-40% of Herba Cistanches raw material, containing a large amount of dietary fibers; At present, only having the CN104957640A preparation method reporting Herba Cistanches dietary fiber, the method takes fresh herba cistanches, after cleaning section, with water according to weight ratio 1:(1-2) mix, pulverize, obtaining Herba Cistanches serosity, serosity is filtrated to get Herba Cistanches slag and Herba Cistanches juice; By Herba Cistanches slag through α-amylase and papain enzymolysis, obtain enzymolysis solution; After Herba Cistanches juice is made leaven, it is linked in enzymolysis solution, ferments; Sucking filtration after fermentation ends, dry, respectively obtain water insoluble dietary fiber and water soluble dietary fiber. Although Herba Cistanches fruit entirely has been utilized by the method, but, do not carry out the higher value application of Herba Cistanches, and this method is only applicable to fresh meat Herba Cistanches, and whole technological process required time is longer, as: the preparation of leaven needs 6-10h, enzymolysis needs 1.5-3h, after leaven inoculation, it is necessary to cultivate 18-22h, precipitate with ethanol needs 1-2h, is unsuitable for extensive use.
At present, the major product of Herba Cistanches is Herba Cistanches dry tablet, water extract (based on polysaccharide) and alcohol extract (based on phenethyl alcohol glycoside), extraction residue in production is not fully utilized, therefore, the present invention is directed to the problems referred to above, it is proposed that the preparation method that a kind of novel enzyme process prepares Herba Cistanches dietary fiber, and use it for auxiliary treatment diabetes and the exploitation of loosening bowel to relieve constipation Related product.
Summary of the invention
For the deficiency that prior art exists, it is an object of the invention to provide Herba Cistanches dietary fiber, its preparation method and application, the preparation method of described Herba Cistanches dietary fiber is simple, water soluble dietary fiber and insoluble dietary fiber can be obtained, it is achieved that the comprehensive utilization of Herba Cistanches, it is simple to commercial production and application from Herba Cistanches residue, and, the Herba Cistanches dietary fiber purity that described preparation method obtains is high, and color and luster is good, has wide application in field of health care food.
For reaching this purpose, the present invention by the following technical solutions:
An object of the present invention is in that the preparation method providing a kind of Herba Cistanches dietary fiber, and described preparation method comprises the steps:
(1) Herba Cistanches residue is mixed with extracting solution, extracted, solid-liquid separation, obtain supernatant and precipitation, described precipitation drying obtains insoluble dietary fiber, and wherein, described extracting solution is any one in acid solution, alkali liquor or enzyme liquid;
(2) supernatant after supernatant or concentration is mixed with dehydrated alcohol carry out precipitate with ethanol, solid-liquid separation, obtain floccule, floccule is dried, obtain water soluble dietary fiber.
The preparation method of Herba Cistanches dietary fiber provided by the invention is simple, can obtain water soluble dietary fiber and insoluble dietary fiber, it is achieved that the comprehensive utilization of Herba Cistanches, it is simple to the commercial production of scale and application.
The described Herba Cistanches residue of step (1) is that the residue after Herba Cistanches water carries, the residue after Herba Cistanches alcohol extraction, Herba Cistanches first carry out water and carry the residue after carrying out alcohol extraction again or Herba Cistanches first carries out alcohol extraction and carries out the combination of any one or at least two in the residue after water proposes again.Typical but non-limiting be combined as: Herba Cistanches water carry after residue and Herba Cistanches alcohol extraction after residue, residue after Herba Cistanches alcohol extraction and Herba Cistanches first carry out water and carry the residue after carrying out alcohol extraction again, Herba Cistanches first carries out alcohol extraction and carries out the residue after water carries and Herba Cistanches again and first carry out water and carry the residue after carrying out alcohol extraction again, residue after Herba Cistanches alcohol extraction, Herba Cistanches first carries out water and carries the residue after carrying out alcohol extraction again and Herba Cistanches and first carry out alcohol extraction and carry out the residue after water carries again, Herba Cistanches water carry after residue, residue after Herba Cistanches alcohol extraction, Herba Cistanches first carries out water and carries the residue after carrying out alcohol extraction again and Herba Cistanches and first carry out alcohol extraction and carry out the residue after water carries again.
Preferably, described Herba Cistanches is the combination of any one or at least two in the fleshy stem of Desert Herba Cistanches, Cistanche Tubulosa, Saline Cistanche Herb, Lanzhou Herba Cistanches or Herba Cistanches sinensis. Typical but non-limiting it is combined as: the fleshy stem of Desert Herba Cistanches and Cistanche Tubulosa, the fleshy stem of Saline Cistanche Herb, Lanzhou Herba Cistanches and Herba Cistanches sinensis, the fleshy stem of Cistanche Tubulosa, Saline Cistanche Herb and Lanzhou Herba Cistanches, the fleshy stem of the fleshy stem of Desert Herba Cistanches, Cistanche Tubulosa and Saline Cistanche Herb, Desert Herba Cistanches, Cistanche Tubulosa, Saline Cistanche Herb, Lanzhou Herba Cistanches and Herba Cistanches sinensis.
The described Herba Cistanches residue of step (1) mixes with extracting solution after smashing again. The granularity of the Herba Cistanches residue after described pulverizing is preferably 100-600 order. Described pulverizing is conducive to Herba Cistanches residue better to mix with extracting solution, shortens extraction time.
Preferably, step (1) described acid solution is the combination of any one or at least two in hydrochloric acid, formic acid, acetic acid, citric acid, malic acid, oxalic acid, sulphuric acid, nitric acid or lactic acid, typical but non-limiting it is combined as: hydrochloric acid and formic acid, acetic acid and citric acid, citric acid, malic acid and oxalic acid, acetic acid, citric acid and malic acid, oxalic acid, sulphuric acid, nitric acid and lactic acid. The volume ratio being preferably the mix acid liquor of hydrochloric acid and formic acid citric acid with lactic acid, hydrochloric acid and formic acid is preferably 1-4:1, if volume ratio is 2:1 or 3:1 etc.
Preferably, the concentration of step (1) described acid solution is 0.1%-10%, such as 0.2%, 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8% or 9% etc., it is preferred to 0.5%-5%.
Preferably, 8-30 times that addition is Herba Cistanches mass of residue of step (1) described acid solution, such as 9 times, 10 times, 12 times, 15 times, 18 times, 20 times, 22 times, 25 times or 28 times etc., it is preferred to 10-20 times.
Preferably, the extraction time of step (1) described acid solution is 1-3h, such as 2h or 2.5h etc.
Step (1) described alkali liquor is NaOH solution and/or KOH solution, it is preferred to the volume ratio of the mixed alkali liquor of NaOH solution and KOH solution, NaOH solution and KOH solution is preferably 1:1.
Preferably, the concentration of described alkali liquor is 0.5%-10%, such as 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 5%, 6%, 7%, 8% or 9% etc., it is preferred to 0.5%-3%.
Preferably, 8-30 times that addition is Herba Cistanches mass of residue of step (1) described alkali liquor, such as 9 times, 10 times, 12 times, 15 times, 18 times, 20 times, 22 times, 25 times or 28 times etc., it is preferred to 10-20 times.
Preferably, the extraction time of step (1) described alkali liquor is 1-3h, such as 2h or 2.5h etc.
The described enzyme liquid of step (1) is the buffer containing hydrolytic enzyme, and described hydrolytic enzyme is amylase, protease and glucosidase.
Preferably, Herba Cistanches residue described in step (1) first mixes with buffer, is sequentially carried out amylase enzymolysis, protease hydrolyzed and glucosidase enzymolysis afterwards. Raw material will be extracted first mix with buffer, it is possible to provide a good reaction buffer system for follow-up enzymatic reaction; Secondly, the macromole such as the starch in raw material and glycogen are hydrolyzed into oligosaccharide by amylase, and proteolysis is become micromolecular aminoacid by protease, and glucosidase is then by diastatic product, namely oligosaccharide is hydrolyzed into glucose, is finally beneficial to the raising of dietary fiber purity and color and luster.
Preferably, the condition of described amylase enzymolysis is 50-95 DEG C, such as 52 DEG C, 53 DEG C, 54 DEG C, 55 DEG C, 56 DEG C, 58 DEG C, 60 DEG C, 62 DEG C, 65 DEG C, 68 DEG C, 70 DEG C, 75 DEG C, 80 DEG C, 85 DEG C or 90 DEG C etc., 20-60min, such as 22min, 25min, 28min, 30min, 32min, 35min, 40min, 50min or 55min etc.
Preferably, the condition of described protease hydrolyzed is 40-70 DEG C, such as 42 DEG C, 45 DEG C, 48 DEG C, 50 DEG C, 52 DEG C, 55 DEG C, 58 DEG C, 60 DEG C, 62 DEG C, 65 DEG C or 68 DEG C etc., 20-60min, such as 22min, 25min, 28min, 30min, 32min, 35min, 40min, 50min or 55min etc.
Preferably, the condition of described glucosidase enzymolysis is 40-60 DEG C, such as 42 DEG C, 45 DEG C, 48 DEG C, 50 DEG C, 52 DEG C, 55 DEG C or 58 DEG C etc., 20-60min, such as 22min, 25min, 28min, 30min, 32min, 35min, 40min, 50min or 55min etc.
Preferably, the addition of described amylase, protease and glucosidase independently be 100-3000U/g, such as 200U/g, 500U/g, 800U/g, 1000U/g, 1200U/g, 1500U/g, 1800U/g, 2000U/g, 2300U/g, 2500U/g or 2800U/g etc., it is preferred to 2000U/g.
Preferably, described buffer is the combination of any one or at least two in phosphate buffer, Tris-HCl buffer or citrate buffer solution, typical but non-limiting it is combined as: phosphate buffer and Tris-HCl buffer, phosphate buffer and citrate buffer solution, phosphate buffer, Tris-HCl buffer and citrate buffer solution, it is preferred to the combination of phosphate buffer and Tris-HCl buffer.
Preferably, the pH value of described buffer is 6.5-8.5, such as 6.8,7.0,7.2,7.5,7.8,8.0,8.2 or 8.4 etc.
Step (1) described solid-liquid separation is the operation that this area is conventional, and typical but non-limiting solid-liquid separation method can be centrifugation.
Step (1) is described dries the routine operation into this area, and those skilled in the art can select described dry temperature according to practical situation.
The addition of step (2) described dehydrated alcohol is 3-5 times of supernatant volume after supernatant volume or concentration, such as 3.5 times, 4 times or 4.5 times etc.
Step (2) is described dries the routine operation into this area, and those skilled in the art can select described dry temperature according to practical situation.
Step (2) described solid-liquid separation is the operation that this area is conventional, and typical but non-limiting solid-liquid separation method can be centrifugal or filter.
As preferred technical scheme, the preparation method that the invention provides a kind of Herba Cistanches dietary fiber, described preparation method comprises the steps:
(1) Herba Cistanches residue is mixed with the buffer that pH value is 6.5-8.5, afterwards, be added thereto to amylase, at 50-95 DEG C of enzymolysis 20-60min;Then, protease is added, at 40-70 DEG C of enzymolysis 20-60min; Adding glucosidase, at 40-60 DEG C of enzymolysis 20-60min, obtain enzymolysis solution, wherein, the addition of described amylase, protease and glucosidase independently be 100-3000U/g; By enzymolysis solution solid-liquid separation, obtaining supernatant and precipitation, described precipitation drying obtains insoluble dietary fiber;
(2) being mixed with dehydrated alcohol by the supernatant after supernatant or concentration and carry out precipitate with ethanol, obtain floccule, wherein, the addition of dehydrated alcohol is 3-5 times of supernatant or the supernatant volume after concentrating; Floccule is dried, obtains water soluble dietary fiber.
Adopt the Herba Cistanches dietary fiber color and luster that enzymatic isolation method obtains better, be creamy white, the superior activity of its functions of loosening bowel relieving constipation and Inhibiting α-glucosidase.
An object of the present invention also resides in provides a kind of Herba Cistanches dietary fiber utilizing described preparation method to prepare, and described Herba Cistanches dietary fiber includes water soluble dietary fiber and insoluble dietary fiber.
An object of the present invention also resides in the application providing a kind of Herba Cistanches dietary fiber utilizing described preparation method to prepare in food or biomedicine field.
Described Herba Cistanches dietary fiber can be used for preparing auxiliary treatment diabetes, auxiliary treatment loosening bowel to relieve constipation or improving food or the health preparation of constipation, and described health preparation includes tablet, electuary, pill, granule, tea beverage, capsule, oral liquid or suspensoid etc.
Compared with prior art, the invention have the benefit that
(1) preparation method of Herba Cistanches dietary fiber provided by the invention is simple, can extract from Herba Cistanches and prepare water soluble dietary fiber and insoluble dietary fiber residue, it is achieved that the comprehensive utilization of Herba Cistanches, it is simple to the commercial production of scale, application;
(2) the Herba Cistanches dietary fiber color and luster that the preparation method of Herba Cistanches dietary fiber provided by the invention prepares is better, it is creamy white, there is loosening bowel to relieve constipation and the Inhibiting α-glucosidase activity of excellence, the functional health-care food of auxiliary treatment diabetes and effect of relaxing bowel is may be made with, it is also possible to add adjuvant and make various oral agents according to the technological requirement of Modern preparations.
Detailed description of the invention
Further illustrating technical scheme by embodiment below, to be best understood from the present invention, its advantage and effect, but described embodiment is merely to illustrate the present invention rather than the restriction present invention.
Embodiment 1: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Desert Herba Cistanches, weigh 500g, add 6000mL distilled water, extract 1h, filter, residue is dried, and obtains Desert Herba Cistanches water and carries residue.
(1) Desert Herba Cistanches water carries residue pulverize, add its 8 times of weight 0.1% hydrochloric acid solution, mixing, extract 3h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 2h will be added, obtain floccule, floccule is dried, obtain water soluble dietary fiber.
Embodiment 2: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Cistanche Tubulosa, weigh 200g, add the alcoholic solution of 1800mL50%, extract 70min, filter, residue is dried, and obtains Cistanche Tubulosa alcohol extraction residue.
(1) Cistanche Tubulosa alcohol extraction residue is pulverized, add its 30 times of weight 10% lactic acid solution, mixing, extract 2h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 5 times of volumes, precipitate with ethanol 4h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 3: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Saline Cistanche Herb, weigh 1000g, add 10000mL distilled water, extract 5h, filter, residue is dried, and obtains Saline Cistanche Herb water and carries residue.
(1) Saline Cistanche Herb water carries residue pulverize, add its 10 times of weight 5% mixed acid (volume ratio the is 1:1) solution of hydrochloric acid and formic acid, mixing, extract 1h, extracting solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 5h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 4: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Herba Cistanches sinensis, weigh 600g, add the alcoholic solution of 9000mL60%, extract 90min, filter, residue is dried, and obtains Herba Cistanches sinensis alcohol extraction residue.
(1) Herba Cistanches sinensis alcohol extraction residue is pulverized, add its 20 times of weight 0.5% sulfuric acid solution, mixing, extract 1.5h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 5 times of volumes, precipitate with ethanol 4h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 5: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Desert Herba Cistanches, weigh 500g, add 6000mL distilled water, extract 1h, filter, residue is dried, and obtains Desert Herba Cistanches water and carries residue.
(1) Desert Herba Cistanches water carries residue pulverize, add the phosphate buffer (pH8.0) of its 12 times of weight, mixing, add the amylase of 100U/g, 50 DEG C, enzymolysis 60min; Add the protease of 500U/g afterwards, 40 DEG C, enzymolysis 60min; Add the glucosidase of 2500U/g, 40 DEG C, enzymolysis 60min, obtain enzymolysis solution; Enzymolysis solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 2h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 6: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Cistanche Tubulosa, weigh 300g, add 5000mL distilled water, extract 2h, centrifugal, residue is dried, and obtains Cistanche Tubulosa water and carries residue.
(1) Cistanche Tubulosa water carries residue pulverize, add the Tris-HCl buffer (pH7.5) of its 16 times of weight, mixing; Add the amylase of 3000U/g, 95 DEG C, enzymolysis 20min; Afterwards, add the protease of 100U/g, 70 DEG C, enzymolysis 20min; Add the glucosidase of 500U/g, 60 DEG C, enzymolysis 20min, obtain enzymolysis solution; Enzymolysis solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 3 times of volumes, precipitate with ethanol 3h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 7: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Saline Cistanche Herb, weigh 200g, add the alcoholic solution of 2000mL50%, extract 50min, filter, residue is dried, and obtains Saline Cistanche Herb alcohol extraction residue.
(1) Saline Cistanche Herb alcohol extraction residue is pulverized, add the citrate buffer solution (pH6.5) of its 18 times of weight, mixing; Add the amylase of 2000U/g, 60 DEG C, enzymolysis 50min; Add the protease of 3000U/g afterwards, 55 DEG C, enzymolysis 40min; Add the glucosidase of 100U/g, 50 DEG C, enzymolysis 40min, obtain enzymolysis solution; Enzymolysis solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 5 times of volumes, precipitate with ethanol 4h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 8: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Lanzhou Herba Cistanches, weigh 1000g, add the alcoholic solution of 20000mL40%, extract 3h, centrifugal, residue is dried, and obtains Lanzhou Herba Cistanches alcohol extraction residue.
(1) Lanzhou Herba Cistanches alcohol extraction residue is pulverized, add the phosphate buffer of its 20 times of weight and the mixed liquor (pH7.0) of Tris-HCl buffer, mixing; Add the amylase of 1550U/g, 72.5 DEG C, enzymolysis 40min; Add the protease of 2000U/g afterwards, 60 DEG C, enzymolysis 50min; Add the glucosidase of 3000U/g, 50 DEG C, enzymolysis 30min, obtain enzymolysis solution; Enzymolysis solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 5h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 9: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Herba Cistanches sinensis, weigh 800g, add the alcoholic solution of 9000mL60%, extract 1h, centrifugal, residue is dried, and obtains Herba Cistanches sinensis alcohol extraction residue.
(1) Herba Cistanches sinensis alcohol extraction residue is pulverized, add the phosphate buffer (pH8.5) of its 25 times of weight, mixing; Add the amylase of 1000U/g, 80 DEG C, enzymolysis 30min; Add the protease of 1550U/g afterwards, 55 DEG C, enzymolysis 40min; Add the glucosidase of 2000U/g, 55 DEG C, enzymolysis 30min, obtain enzymolysis solution; Enzymolysis solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 2h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 10: the preparation of dietary fiber in Herba Cistanches residue
Being pulverized by Desert Herba Cistanches, weigh 5000g, add the water of 100000mL, extract 1.5h, centrifugal, residue is dried, and obtains Desert Herba Cistanches water and carries residue; Then adding the alcoholic solution of 60000mL50%, extract 1h, filter, residue is dried, and obtains Desert Herba Cistanches alcohol extraction residue.
(1) Desert Herba Cistanches alcohol extraction residue is pulverized, add the phosphate buffer (pH8.2) of its 26 times of weight, mixing; Add the amylase of 2500U/g, 75 DEG C, enzymolysis 35min; Add the protease of 1000U/g afterwards, 45 DEG C, enzymolysis 30min; Add the glucosidase of 1550U/g, 50 DEG C, enzymolysis 50min, obtain enzymolysis solution; Enzymolysis solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(1) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 2h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 11: the preparation of dietary fiber in Herba Cistanches residue
Being pulverized by Desert Herba Cistanches, weigh 5000g, add the alcoholic solution of 10000mL52%, extract 1h, centrifugal, residue is dried, and obtains Desert Herba Cistanches alcohol extraction residue.Then adding 80000mL water, extract 1h, filter, residue is dried, and obtains Desert Herba Cistanches water and carries residue.
(1) Desert Herba Cistanches water carries residue pulverize, add the Tris-HCl buffer (pH8.0) of its 28 times of weight, mixing; Add the amylase of 500U/g, 65 DEG C, enzymolysis 55min; Add the protease of 2500U/g afterwards, 65 DEG C, enzymolysis 55min; Add the glucosidase of 1000U/g, 60 DEG C, enzymolysis 35min, obtain enzymolysis solution; Enzymolysis solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(1) after supernatant concentration, the dehydrated alcohol of its 4.5 times of volumes, precipitate with ethanol 1.5h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 12: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Saline Cistanche Herb, weigh 1000g, add 10000mL distilled water, extract 5h, filter, residue is dried, and obtains Saline Cistanche Herb water and carries residue.
(1) Saline Cistanche Herb water carries residue pulverize, add its 15 times of weight 2.5% citric acid and the mixed acid solution of lactic acid, mixing, extract 2h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 3 times of volumes, precipitate with ethanol 5h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 13: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Saline Cistanche Herb, weigh 1000g, add 10000mL distilled water, extract 5h, filter, residue is dried, and obtains Saline Cistanche Herb water and carries residue.
(1) Saline Cistanche Herb water carries residue pulverize, add its 15 times of weight 5% mixed acid (volume ratio the is 4:1) solution of hydrochloric acid and formic acid, mixing, extract 2.5h, extracting solution is centrifuged, obtains supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 3 times of volumes, precipitate with ethanol 5h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 14: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Cistanche Tubulosa, weigh 1000g, add 10000mL distilled water, extract 5h, filter, residue is dried, and obtains Cistanche Tubulosa water and carries residue.
(1) Cistanche Tubulosa water carries residue pulverize, add its 25 times of weight 3% mixed acid (volume ratio the is 2.5:1) solution of hydrochloric acid and formic acid, mixing, extract 2.5h, extracting solution is centrifuged, obtain supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 5 times of volumes, precipitate with ethanol 5h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 15: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Desert Herba Cistanches, weigh 500g, add 6000mL distilled water, extract 1h, filter, residue is dried, and obtains Desert Herba Cistanches water and carries residue.
(1) Desert Herba Cistanches water carries residue pulverize, add its 8 times of weight 0.5% NaOH solution, mixing, extract 1h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 2h will be added, obtain floccule, floccule is dried, obtain water soluble dietary fiber.
Embodiment 16: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Cistanche Tubulosa, weigh 200g, add the alcoholic solution of 1800mL50%, extract 70min, filter, residue is dried, and obtains Cistanche Tubulosa alcohol extraction residue.
(1) Cistanche Tubulosa alcohol extraction residue is pulverized, add its 10 times of weight 10% KOH solution, mixing, extract 3h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 5 times of volumes, precipitate with ethanol 4h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 17: Herba Cistanches water proposes the preparation of dietary fiber in residue
Being pulverized by Saline Cistanche Herb, weigh 1000g, add 10000mL distilled water, extract 5h, filter, residue is dried, and obtains Saline Cistanche Herb water and carries residue.
(1) Saline Cistanche Herb water carries residue pulverize, add its 20 times of weight 3% NaOH solution and the mixed alkali liquor (volume ratio is 1:1) of KOH solution, mixing, extract 2h, extracting solution is centrifuged, obtain supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4 times of volumes, precipitate with ethanol 5h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 18: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Herba Cistanches sinensis, weigh 600g, add the alcoholic solution of 9000mL60%, extract 90min, filter, residue is dried, and obtains Herba Cistanches sinensis alcohol extraction residue.
(1) Herba Cistanches sinensis alcohol extraction residue is pulverized, add its 30 times of weight 1.75% NaOH solution, mixing, extract 1.5h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 3 times of volumes, precipitate with ethanol 4h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 19: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Cistanche Tubulosa, weigh 200g, add the alcoholic solution of 1800mL50%, extract 70min, filter, residue is dried, and obtains Cistanche Tubulosa alcohol extraction residue.
(1) Cistanche Tubulosa alcohol extraction residue is pulverized, add its 15 times of weight 5.25% KOH solution, mixing, extract 2.5h, extracting solution be centrifuged, obtain supernatant and precipitation, precipitation is dry, obtain insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 3.5 times of volumes, precipitate with ethanol 4h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
Embodiment 20: the preparation of dietary fiber in Herba Cistanches alcohol extraction residue
Being pulverized by Cistanche Tubulosa, weigh 200g, add the alcoholic solution of 1800mL50%, extract 70min, filter, residue is dried, and obtains Cistanche Tubulosa alcohol extraction residue.
(1) Cistanche Tubulosa alcohol extraction residue is pulverized, add its 19 times of weight 6% the mixed alkali liquor (volume ratio is 2:1) of NaOH solution and KOH solution, mixing, extract 2h, extracting solution is centrifuged, obtain supernatant and precipitation, precipitation is dried, obtains insoluble dietary fiber;
(2) after supernatant concentration, the dehydrated alcohol of its 4.5 times of volumes, precipitate with ethanol 4h will be added, obtain floccule, dry, obtain water soluble dietary fiber.
The loosening bowel to relieve constipation experiment of Herba Cistanches dietary fiber
In Example 8, the dietary fiber of preparation carries out intestinal motility experiment.
Animal: ICR male mice, body weight 32-38g, often group 10, buy and tie up laboratory animal company limited of tonneau China in Beijing.
Experiment sets blank group, model group, positive controls and three dosage groups. After given the test agent 14 days, the mice fasting of each group be can't help water 16h, model group, positive controls and three dosage group gavages and given compound diphenoxylate (10mg/kg), and blank group gives distilled water.
After compound diphenoxylate 0.5h, dosage component does not give 5% activated carbon (containing 10% Radix Acaciae senegalis) containing corresponding given the test agent, and blank group and model group are to activated carbon gavage, positive controls gavage nigalax (bisacodyl).
Activated carbon gavage is de-cervical vertebra execution mice after 25 minutes, open abdominal cavity separation mesentery, clip upper end from pylorus, lower end to the intestinal tube of ileocecus, it is placed on pallet, gently small intestinal is pulled into straight line, measuring Length of intestine is " total small intestinal length ", is " activated carbon propelling length " from pylorus to activated carbon forward position, is calculated as follows activated carbon propelling rate:
Activated carbon propelling rate (%)=activated carbon advances length (cm)/total small intestinal length (cm) × 100%
Experimental result is as shown in table 1.
The impact on mice with constipation intestinal motility of the table 1 Herba Cistanches dietary fiber
* P < 0.05: compared with model group, significant difference
More than test result indicate that: Herba Cistanches dietary fiber can promote the propelling of activated carbon in mice with constipation small intestinal, and presents dose dependent, illustrate that Herba Cistanches dietary fiber has promotion small intestinal peristalsis, the effect of loosening bowel to relieve constipation.
The Inhibiting α-glucosidase activity of Herba Cistanches dietary fiber
In Example 10, the dietary fiber of preparation carries out Inhibiting α-glucosidase activity experiment.
96 orifice plates add the phosphate buffered solution 50 μ L of 50mmol/LpH6.8,5mmol/L reduced glutathione 2.5 μ L, 0.2U/mL alpha-glucosidase 2.5 μ L, it is separately added into the inhibitor 10 μ L of variable concentrations, after mixing in the calorstat of 37 DEG C constant temperature 10min, add 20mmol/LpNPG5 μ L, then put constant temperature 10min in the calorstat of 37 DEG C, add 0.1mol/LNa2CO350 μ L terminate reaction, measure the absorbance at 405nm place by microplate reader. Blank group without inhibitor, blank background group is not added with pNPG and inhibitor, it is suppressed that agent background group is not added with pNPG, supplies volume by distilled water, and other operations are with inhibitor group. Alpha-glucosidase activity suppression ratio is calculated as follows by inhibitor:
Suppression ratio (%)=[(A1-A2)-(A3-A4)]/(A1-A2) × 100
Wherein: A1 is blank group absorbance, and A2 is blank background group absorbance, and A3 is inhibitor group absorbance, and A4 is inhibitor background group absorbance.
The table 2 Herba Cistanches dietary fiber suppression ratio to alpha-glucosidase
| Dietary fiber concentration | Suppression ratio (%) |
| 1 | 23.06 |
| 2 | 37.27 |
| 3 | 41.02 |
| 4 | 48.26 |
| 5 | 56.20 |
Visible, alpha-glucosidase is had obvious inhibition by Herba Cistanches dietary fiber, and along with the increase of dietary fiber concentration, the inhibition of alpha-glucosidase is strengthened gradually.
The Herba Cistanches dietary fiber that other embodiments prepares has loosening bowel to relieve constipation and the Inhibiting α-glucosidase activity of excellence equally.
Applicant states; the foregoing is only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; person of ordinary skill in the field it should be understood that; any those skilled in the art of belonging to are in the technical scope that the invention discloses; the change that can readily occur in or replacement, all fall within protection scope of the present invention and open scope.
Claims (10)
1. the preparation method of a Herba Cistanches dietary fiber, it is characterised in that described preparation method comprises the steps:
(1) Herba Cistanches residue is mixed with extracting solution, extracted, solid-liquid separation, obtain supernatant and precipitation, described precipitation drying obtains insoluble dietary fiber, and wherein, described extracting solution is any one in acid solution, alkali liquor or enzyme liquid;
(2) supernatant after supernatant or concentration is mixed with dehydrated alcohol carry out precipitate with ethanol, solid-liquid separation, obtain floccule, floccule is dried, obtain water soluble dietary fiber.
2. preparation method according to claim 1, it is characterized in that, the described Herba Cistanches residue of step (1) is that the residue after Herba Cistanches water carries, the residue after Herba Cistanches alcohol extraction, Herba Cistanches first carry out water and carry the residue after carrying out alcohol extraction again or Herba Cistanches first carries out alcohol extraction and carries out the combination of any one or at least two in the residue after water proposes again;
Preferably, described Herba Cistanches is the combination of any one or at least two in the fleshy stem of Desert Herba Cistanches, Cistanche Tubulosa, Saline Cistanche Herb, Lanzhou Herba Cistanches or Herba Cistanches sinensis.
3. preparation method according to claim 1 and 2, it is characterised in that the described Herba Cistanches residue of step (1) mixes with extracting solution after smashing again;
Preferably, step (1) described acid solution is the combination of any one or at least two in hydrochloric acid, formic acid, acetic acid, citric acid, malic acid, oxalic acid, sulphuric acid, nitric acid or lactic acid, the volume ratio being preferably the mix acid liquor of hydrochloric acid and formic acid, hydrochloric acid and formic acid is preferably 1-4:1;
Preferably, the concentration of step (1) described acid solution is 0.1%-10%, it is preferred to 0.5%-5%;
Preferably, 8-30 times that addition is Herba Cistanches mass of residue of step (1) described acid solution, it is preferred to 10-20 times;
Preferably, the extraction time of step (1) described acid solution is 1-3h.
4. according to the preparation method one of claim 1-3 Suo Shu, it is characterized in that, step (1) described alkali liquor is NaOH solution and/or KOH solution, it is preferred to the volume ratio of the mixed alkali liquor of NaOH solution and KOH solution, NaOH solution and KOH solution is preferably 1:1;
Preferably, the concentration of described alkali liquor is 0.5%-10%, it is preferred to 0.5%-3%;
Preferably, 8-30 times that addition is Herba Cistanches mass of residue of step (1) described alkali liquor, it is preferred to 10-20 times;
Preferably, the extraction time of step (1) described alkali liquor is 1-3h.
5. according to the preparation method one of claim 1-4 Suo Shu, it is characterised in that the described enzyme liquid of step (1) is the buffer containing hydrolytic enzyme, and described hydrolytic enzyme is amylase, protease and glucosidase;
Preferably, Herba Cistanches residue described in step (1) first mixes with buffer, is sequentially carried out amylase enzymolysis, protease hydrolyzed and glucosidase enzymolysis afterwards;
Preferably, the condition of described amylase enzymolysis is 50-95 DEG C, 20-60min;
Preferably, the condition of described protease hydrolyzed is 40-70 DEG C, 20-60min;
Preferably, the condition of described glucosidase enzymolysis is 40-60 DEG C, 20-60min;
Preferably, the addition of described amylase, protease and glucosidase independently be 100-3000U/g, it is preferred to 2000U/g;
Preferably, described buffer is the combination of any one or at least two in phosphate buffer, Tris-HCl buffer or citrate buffer solution, it is preferred to the combination of phosphate buffer and Tris-HCl buffer;
Preferably, the pH value of described buffer is 6.5-8.5.
6. according to the preparation method one of claim 1-5 Suo Shu, it is characterised in that the addition of step (2) described dehydrated alcohol is 3-5 times of supernatant volume after supernatant volume or concentration.
7. according to the preparation method one of claim 1-6 Suo Shu, it is characterised in that described preparation method comprises the steps:
(1) Herba Cistanches residue is mixed with the buffer that pH value is 6.5-8.5, afterwards, be added thereto to amylase, at 50-95 DEG C of enzymolysis 20-60min;Then, protease is added, at 40-70 DEG C of enzymolysis 20-60min; Adding glucosidase, at 40-60 DEG C of enzymolysis 20-60min, obtain enzymolysis solution, wherein, the addition of described amylase, protease and glucosidase independently be 100-3000U/g; By enzymolysis solution solid-liquid separation, obtaining supernatant and precipitation, described precipitation drying obtains insoluble dietary fiber;
(2) being mixed with dehydrated alcohol by the supernatant after supernatant or concentration and carry out precipitate with ethanol, obtain floccule, wherein, the addition of dehydrated alcohol is 3-5 times of supernatant or the supernatant volume after concentrating; Floccule is dried, obtains water soluble dietary fiber.
8. the Herba Cistanches dietary fiber obtained according to the preparation method one of claim 1-7 Suo Shu, described Herba Cistanches dietary fiber includes water soluble dietary fiber and insoluble dietary fiber.
9. the Herba Cistanches dietary fiber according to claim 8 application in food or biomedicine field.
10. the application of Herba Cistanches dietary fiber according to claim 9, it is characterised in that described Herba Cistanches dietary fiber is for preparing auxiliary treatment diabetes, auxiliary treatment loosening bowel to relieve constipation or improving food or the health preparation of constipation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610079178.9A CN105661556A (en) | 2016-02-04 | 2016-02-04 | Herba cistanche dietary fibers and preparation method and application thereof |
Applications Claiming Priority (1)
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| CN106108030A (en) * | 2016-07-05 | 2016-11-16 | 江苏大学 | Garlic skin dietary fiber and twin-screw extruder thereof and enzymolysis modified technology of preparing and application |
| CN106389541A (en) * | 2016-11-02 | 2017-02-15 | 杏辉天力（杭州）药业有限公司 | Cistanche deserticola extract and preparation method and application thereof |
| CN106490627A (en) * | 2016-10-27 | 2017-03-15 | 葛海鸿 | Extract the enzyme chemical method of beggar-ticks dietary fiber |
| CN106579465A (en) * | 2016-11-02 | 2017-04-26 | 华南理工大学 | Sisal hemp residue dietary fibers and preparation method thereof |
| CN108244660A (en) * | 2018-03-02 | 2018-07-06 | 中国科学院过程工程研究所 | A kind of okra dietary fiber and its preparation method and application |
| CN113729232A (en) * | 2021-09-08 | 2021-12-03 | 山东乐道农业科技有限公司 | Preparation method of cistanche deserticola dietary fiber |
| CN114515008A (en) * | 2022-03-04 | 2022-05-20 | 广东青云山药业有限公司 | Cistanche tubulosa extract and preparation method thereof |
| CN114588201A (en) * | 2022-03-30 | 2022-06-07 | 北京中科健宇生物科技有限公司 | Preparation method of cistanche deserticola extract and alcohol effect relieving and liver protecting product containing cistanche deserticola extract |
| CN115926011A (en) * | 2022-11-01 | 2023-04-07 | 大连康芝源生物科技有限公司 | Edible fungus polysaccharide extraction method and application thereof in food production |
| CN116076744A (en) * | 2023-03-13 | 2023-05-09 | 塔里木大学 | Cistanche deserticola dietary fiber and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106108030A (en) * | 2016-07-05 | 2016-11-16 | 江苏大学 | Garlic skin dietary fiber and twin-screw extruder thereof and enzymolysis modified technology of preparing and application |
| CN106490627A (en) * | 2016-10-27 | 2017-03-15 | 葛海鸿 | Extract the enzyme chemical method of beggar-ticks dietary fiber |
| CN106389541A (en) * | 2016-11-02 | 2017-02-15 | 杏辉天力（杭州）药业有限公司 | Cistanche deserticola extract and preparation method and application thereof |
| CN106579465A (en) * | 2016-11-02 | 2017-04-26 | 华南理工大学 | Sisal hemp residue dietary fibers and preparation method thereof |
| CN108244660A (en) * | 2018-03-02 | 2018-07-06 | 中国科学院过程工程研究所 | A kind of okra dietary fiber and its preparation method and application |
| CN113729232A (en) * | 2021-09-08 | 2021-12-03 | 山东乐道农业科技有限公司 | Preparation method of cistanche deserticola dietary fiber |
| CN113729232B (en) * | 2021-09-08 | 2023-06-23 | 山东乐道农业科技有限公司 | Preparation method of cistanche deserticola dietary fiber |
| CN114515008A (en) * | 2022-03-04 | 2022-05-20 | 广东青云山药业有限公司 | Cistanche tubulosa extract and preparation method thereof |
| CN114588201A (en) * | 2022-03-30 | 2022-06-07 | 北京中科健宇生物科技有限公司 | Preparation method of cistanche deserticola extract and alcohol effect relieving and liver protecting product containing cistanche deserticola extract |
| CN114588201B (en) * | 2022-03-30 | 2024-01-02 | 北京中科健宇生物科技有限公司 | Preparation method of cistanche extract and anti-alcohol liver-protecting product containing cistanche extract |
| CN115926011A (en) * | 2022-11-01 | 2023-04-07 | 大连康芝源生物科技有限公司 | Edible fungus polysaccharide extraction method and application thereof in food production |
| CN116076744A (en) * | 2023-03-13 | 2023-05-09 | 塔里木大学 | Cistanche deserticola dietary fiber and preparation method thereof |
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