Summary of the invention
Technical problem to be solved by this invention is a kind of method promoting Penaeus Vannmei nursery seedling amount alive, to overcome the deficiency that prior art exists.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
Promote Penaeus Vannmei nursery to live the method for seedling amount, it is characterized in that: every day full pool spilling head dosage to be the breeding water body Tiny ecosystem bactericide of 1.5-2.5ppm and dosage be 0.7-1.2ppm breeding water body substrate modifier;
The former powder of the dense bacteriophagic Bdellovibrio of height, concentration that the concentration that described breeding water body Tiny ecosystem bactericide is 4:2:3 by mass ratio is greater than 3,300,000,000/gram are greater than 2,000,000,000/gram of bacillus amyloliquefaciens microcapsules, effective content is greater than 99% soluble starch and forms;
The former powder of bacillus megaterium, effective content that the former powder of bacillus licheniformis, concentration that the former powder of bacillus subtilis, concentration that the concentration that described breeding water body base stock modifying agent is 2:2:2:4 by mass ratio is greater than 10,000,000,000/gram are greater than 8,000,000,000/gram are greater than 6,000,000,000/gram are greater than 99% soluble starch and form.
In addition, the present invention also provides a kind of method promoting culture of Penaeus vannamei output, it is characterized in that:
Every morning, noon, afternoon respectively to throw something and feed a feed to Penaeus Vannmei in pond; Do not mix breeding water body Tiny ecosystem bactericide in feed in the morning; Noon and evening mix breeding water body Tiny ecosystem bactericide in feed, dosage is that 100 kg diet mix 220-230g breeding water body Tiny ecosystem bactericide thoroughly; The former powder of the dense bacteriophagic Bdellovibrio of height, concentration that the concentration that described breeding water body Tiny ecosystem bactericide is 4:2:3 by mass ratio is greater than 3,300,000,000/gram are greater than 2,000,000,000/gram of bacillus amyloliquefaciens microcapsules, effective content is greater than 99% soluble starch Homogeneous phase mixing and forms.
When vibrios yellow color colonies quantity in water body higher than the green colony counts of 10000/ml or vibrios higher than 100/ml time, adopt every 2 mu of waters surface immediately, dosage full pool spilling head breeding water body Tiny ecosystem bactericide that 1 meter of depth of water splashes 227g breeding water body Tiny ecosystem bactericide.
When Penaeus Vannmei in breeding process occur arbitrary in jejunum, empty stomach, red palpus, disconnected palpus, red tail, red leg or combine symptom time, mix the dosage of raising breeding water body Tiny ecosystem bactericide of throwing something and feeding in noon every day, evening and increase to 30 kg diet and mix 220-230g breeding water body Tiny ecosystem bactericide thoroughly; After transference cure, recover normal dose.
Adopt technique scheme, the present invention has the effect promoting Penaeus Vannmei nursery seedling amount alive and cultured output, more remarkable effect compared with conventional method, its application is more conducive to the use reducing the chemosterilant such as formalin, bleaching powder in breeding process, alleviates the secondary pollution of use to cultivate water of chemosterilant.
Embodiment
One, experiment material
1, breeding water body Tiny ecosystem bactericide, the former powder of the dense bacteriophagic Bdellovibrio of height, the concentration that are greater than 3,300,000,000/gram by concentration are greater than 2,000,000,000/gram of bacillus amyloliquefaciens microcapsules, effective content is greater than 99% soluble starch and forms, and wherein the mass ratio of the former powder of high dense bacteriophagic Bdellovibrio, bacillus amyloliquefaciens microcapsules, soluble starch is 4:2:3.
Its preparation method is first by the uniform stirring mixing at normal temperatures of bacillus amyloliquefaciens microcapsules, soluble starch, mixing speed is 30r/min, mixing time is 30min, and then add the former powder of high dense bacteriophagic Bdellovibrio Homogeneous phase mixing at normal temperatures, mixing speed is 30r/min, mixing time is 60min, is uniformly mixed process twice and is all in air-tight state.
2, breeding water body substrate modifier, the former powder of Bacillusmageterium, effective content that the former powder of bacillus licheniformis, concentration that the former powder of bacillus subtilis, the concentration that are greater than 10,000,000,000/gram by concentration are greater than 8,000,000,000/gram are greater than 6,000,000,000/gram are greater than 99% soluble starch and form, and wherein the mass ratio of the former powder of bacillus subtilis, the former powder of bacillus licheniformis, the former powder of bacillus megaterium (the former powder of Bacillusmageterium), soluble starch is 2:2:2:4.
Its preparation method is first by the uniform stirring mixing at normal temperatures of former for bacillus subtilis powder, the former powder of bacillus licheniformis, the former powder of bacillus megaterium (the former powder of Bacillusmageterium), mixing speed is 30r/min, mixing time is 30min, and then add soluble starch Homogeneous phase mixing at normal temperatures, mixing speed is 30r/min, mixing time is 60min, is uniformly mixed process twice and is all in air-tight state.
3, bleaching powder, effective content is 30%, is purchased from Shandong Tancheng bleaching powder factory.
4, formalin, effective content is 40%, is purchased from Chemical Reagent Co., Ltd., Sinopharm Group.
5, brine shrimp eggs, concentration is 5000/g, is purchased from Hangzhou Cao Shi aquaculture Co., Ltd.
6, litopenaeus vannamei compound feed, is produced by Guangdong Hengxing Group Co., Ltd..
7, TCBS agar medium is dull and stereotyped, for detecting amount of vibrio, for Shanghai Lvao Biotechnology Co., Ltd. produces.
8, the nursery pond of 8 area 18m2, depth of water 1-1.5 rice, 5 phases of throwing in young shrimps, putting seedling amount is 1,500,000/pond, brine shrimp eggs of throwing something and feeding every day, point throws something and feeds for 4 times, and feeding volume is 10,000 worm's ovum/ten thousand shrimp seedlings.
9, the culturing pool of 8 areas 3 mu, depth of water 2.5-3m, it is 110,000 tails/mu that Penaeus Vannmei puts seedling amount, litopenaeus vannamei compound feed of throwing something and feeding every day, and inventory is the 1-3% of shrimp gross weight.
Two, experimental technique
1, the method for Penaeus Vannmei nursery seedling amount alive is promoted
Experiment comprises 4 contrast nursery ponds, 4 test nursery ponds.
Contrast nursery pond only uses formalin, and every two weeks full pool spilling head is sterilized a water body, and full pool spilling head consumption is 100g/ mu rice (i.e. the amount of the water splashing 100g that 1 mu of water surface 1 meter is dark).
Test nursery pond full pool spilling head every day breeding water body Tiny ecosystem bactericide and breeding water body substrate modifier, using dosage of splashing is respectively 2ppm and 1ppm.Test period is 7 days.Add up output of seedling and survival rate after off-test, calculate average output of seedling and average survival.
2, the method for culture of Penaeus vannamei output is promoted
Experiment comprises 4 contrast ponds, 4 test tanks.Contrast pond only uses bleaching powder, and every two weeks full pool spilling head is sterilized a water body, and full pool spilling head consumption is 100g/ mu rice.
The test tank dosage of mixing 227g breeding water body Tiny ecosystem bactericide according to 100 kg diet thoroughly is mixed Penaeus Vannmei and is raised breeding water body Tiny ecosystem bactericide and prevent.
Respectively throw something and feed in every morning (9 point), noon (12 point), afternoon (17 point) feed, morning, breeding water body Tiny ecosystem bactericide do not mixed by feed; Noon and evening feed mix breeding water body Tiny ecosystem bactericide.
Within in breeding process every 7 days, detect vibrios content in water body, when vibrios yellow color colonies quantity in water body higher than the green colony counts of 10000/ml or vibrios higher than 100/ml time, adopt every 2 mu of waters surface immediately, dosage full pool spilling head breeding water body Tiny ecosystem bactericide that 1 meter of depth of water splashes 227g breeding water body Tiny ecosystem bactericide.
When Penaeus Vannmei in breeding process occur arbitrary in jejunum, empty stomach, red palpus, disconnected palpus, red tail, red leg or combine symptom time, mix the dosage of raising breeding water body Tiny ecosystem bactericide of throwing something and feeding in noon every day, evening and increase to 30 kg diet and mix 227g breeding water body Tiny ecosystem bactericide thoroughly; After transference cure, recover normal dose (every 227g breeding water body Tiny ecosystem bactericide spice 100 kilograms).The test culture-cycle is 3 months, and water temperature is 15-28 DEG C.Test cultivation terminates the per mu yield in each pond of rear statistics, calculates per mu yield.
Three, experimental result
1, the lifting effect of Penaeus Vannmei nursery seedling amount alive
Experimental result is in table 1.Experimental result shows, compared with contrast nursery pond, test nursery pond output of seedling improves 30% than contrast nursery pond, and survival rate also improves 20% than contrast nursery pond.
The output of seedling of table 1 Penaeus Vannmei nursery
Project |
Test tank |
Contrast pond |
Pond number (individual) |
4 |
4 |
Put seedling amount (tail/pond) |
1500000 |
1500000 |
Average output of seedling (tail) |
1300000 |
1000000 |
Average survival |
86.66% |
66.6% |
2. the lifting effect of culture of Penaeus vannamei output
Experimental result is in table 2.Experimental result shows, the per mu yield of experimental group Penaeus Vannmei is 1860 jin, improves 40.91% compared with contrast pond.
The cultured output of table 2 Penaeus Vannmei
Project |
Test tank |
Contrast pond |
Pond number (individual) |
4 |
4 |
Put seedling amount (tail/mu) |
110000 |
110000 |
Per mu yield (jin) |
1860 |
1320 |
As can be seen from the above table, method of the present invention is compared with conventional method, and better effects if, safer environmental protection, more not easily produces secondary pollution.
But; those skilled in the art will recognize that; above-mentioned embodiment is exemplary; to better enable those skilled in the art understand this patent; can not be interpreted as it is restriction to this patent protection domain; as long as according to this patent disclose any equivalent change done or the modification of spirit, all fall into the scope of this patent protection.