CN103843704A - Method for cultivating young portunid - Google Patents

Method for cultivating young portunid Download PDF

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Publication number
CN103843704A
CN103843704A CN201310741325.0A CN201310741325A CN103843704A CN 103843704 A CN103843704 A CN 103843704A CN 201310741325 A CN201310741325 A CN 201310741325A CN 103843704 A CN103843704 A CN 103843704A
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crab
pond
bacillus
nursery pond
feeding
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CN103843704B (en
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杨会成
廖妙飞
周宇芳
钟明杰
付万冬
马华威
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Zhejiang Marine Development Research Institute
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Zhejiang Marine Development Research Institute
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention provides a method for cultivating young portunid, comprising the following steps of early-stage preparation: soaking a cultivation pond with clean water for 1-2 days, then sterilizing the cultivation pond with 50ppm-100ppm bleaching powder, paving sterilized sea mud on the bottom of the cultivation pond, introducing sea water and adding bdellovibrio solution and nitrifying bacteria; spawning; after-treatment: throwing bacillus solution during the cultivation process. By the adoption of the method provided by the invention, the water can be purified, the water quality can be improved, and the residues caused by a conventional cultivation method can be prevented; meanwhile, as the water is not changed during the cultivation process, the growing cost is reduced; not only is the survival rate of the young portunid improved, but also the immunity of the young portunid can be improved; with the utilization of the method, the abuse of drugs such as antibiotics in production is avoided; the incidence of diseases is lowered; the production yield is improved.

Description

A kind of method that swimming crab is grown seedlings
Technical field
The present invention relates to technical field of aquaculture, relate in particular to a kind of method that swimming crab is grown seedlings.
 
Background technology
Swimming crab, formal name used at school " decoy ", because carapace is shuttle shape, there are three projections in the central authorities of crust, so claim again Portunus trituberculatus Miers.Portunus trituberculatus Miers build is large, growth is fast, use value is high, delicious meat, nutritious, edible ratio are large, is not only of high nutritive value, but also has the medical values such as heat-clearing, loose blood and enriching yin.Mainly originate in the Huanghai Sea, the Bohai Sea and the East Sea.Due to the raising of people's living standard, marine products crab can not meet the growing demand of people, therefore start artificial feeding.Portunus trituberculatus Miers tradition seedling raising process changes water in a large number, consumes mass energy, and increases production cost.Often pollute natural waters at present, seawater eutrophication, and a large amount of bacteriums, fungus breeding, bring very large impact to aquatic fry growing.In seedling raising process, often change water, the young is in order to shake down, and the energy i (in vivo) of need to beating, reduces for the energy of growing, and has increased like this cultivation time.The change of young living environment can make pathogenic microorganisms increase the threat of the young, just likely causes disease.In order to prevent and treat crab disease, many producers can use a large amount of agricultural chemicals, antibiotic and some high residue chemical synthetic drugs, what have even adds chemical fertilizer and growth acceleration hormone for pursuing growth rate and output in feed, thereby crab Product quality and safety straight line is declined.
Chinese patent publication No. CN 101755696 A, date of publication on June 30th, 2010, name is called the method for Portunus trituberculatus Miers low latitudes season seeding and cultivation, this application case discloses a kind of method of Portunus trituberculatus Miers low latitudes season seeding and cultivation, in August to mid-October, from the natural marine site to the north of the Taiwan Straits or Portunus trituberculatus Miers culturing pool, obtain oogenesis Portunus trituberculatus Miers, ovigerous crab is transported to south (on the south the Taiwan Straits).As Portunus trituberculatus Miers seedling, carry out anti-season cultivation with young crab.The Portunus trituberculatus Miers of north cultivation can be cultivated in south.Its weak point is, changes in a large number water the immunity of swimming crab is reduced in seedling raising process, and cultivation survival rate reduces.
 
Summary of the invention
The object of the invention is between traditional swimming crab nursery stage, to change in a large number water the immunity of swimming crab is reduced in order to solve the defect that cultivation survival rate reduces and a kind of provide swimming crab of improving swimming crab juvenile crab immunity and survival rate to grow seedlings method.
To achieve these goals, the present invention is by the following technical solutions:
The method that swimming crab is grown seedlings, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1-2 days, nursery pond is carried out disinfection with the bleaching powder of 50ppm-100ppm, rinse well with clear water again, after nursery pond dries, the ooze through sterilization that is 4-8cm to nursery pond bottom laying depth, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria liquid, make Bdellovibrio content in seawater reach 10 3-10 6the content of cfu/mL, nitrifying bacteria reaches 10 3-10 4cfu/mL, then shading leaves standstill 5-8 days;
B) lay eggs: transfer grey black, idiosome heartbeat to and reach the 150-160 time/more than min female crab being fertilized to dropping into ovum look in the nursery pond of step a), the cloth seedling density of nursery pond is 15-20 × 10 4individual/m 3;
C) post processing: after having laid eggs, female crab is pulled out, between nursery stage, the bait of regularly throwing something and feeding, threw in a bacillus liquid every 5-7 days, and in seawater, bacillus liquid concentration is 10 2-10 5cfu/mL.
In the technical program, in step a), in larval rearing water, add nitrifying bacteria liquid, nitrifying bacteria is the bacterium of the nitric acid state that mineralized nitrogen poisonous in water can be become to nontoxic, and in the water of nursery pond, add nitrifying bacteria liquid, can make nitrifying bacteria first seawater be purified, the poisonous mineralized nitrogen in seawater is become to the materials such as nitric acid state;
Bdellovibrio is to be arc gram-Negative bacillus, in extensive distribution and waters soil and animal body, and big or small 0.2-0.25 μ m × 0.5-1.4 μ m, extremely single flagellum, motion is active.Bdellovibrio is a kind of bacterium that parasitizes bacterium, can invest on the cell wall of host bacterium with the absorber of self, and promptly pierce in host cell, utilizes nourishing and growing, breeding of host, finally causes the cracking of host bacterium.Bdellovibrio is selective to host, specially taking Gram-negative bacterias such as vibrios, pseudomonad, Aeromonas, tardas as host, cracking ability to pathogenic bacteria is stronger, add Bdellovibrio can prevent growing of the pathogenetic bacteria harmful to crab, effectively avoided the threat of pathogenetic bacteria to the swimming crab young in swimming crab seedling raising process;
Step c) adds bacillus liquid in larval rearing water, bacillus can be that bacillus licheniformis can be also bacillus subtilis, organic matter decomposition power is strong, can produce that abundant metabolism product, antibacterial, the harmful power of going out are strong, the function of deodorizing, in seedling raising process, the ight soil that the young in each period produces can be decomposed by bacillus, has avoided the pollution of water quality, can stimulate the growth to crab young immune organ, strengthen immunity of organisms.
As preferably, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space is laid the thick fine sand of 10-12 cm, and depth of water is controlled at 0.6m-1m, salinity 25 ‰-30 ‰, female crab breeding density be 3-5 only/m 2, by the 5%-8% of body weight throw something and feed shellfish meat or clam worm.
As preferably, while supporting female crab, under natural temperature, stablize 1-2 days, the accelerating that then starts to heat up, to promote production, wintering pond water temperature improves 0.5-0.8 DEG C every day, to 18-20 DEG C of constant temperature.
As preferably, before female crab input nursery pond is laid eggs, with 20mg/L-100mg/L formalin dipping bath 10-15min sterilization, clear water rinses.
As preferably, in step c), bacillus liquid is that dry bacterium powder and brown sugar are added in seawater by weight the ratio of 1:2-4, and activation 6-8h, then filters, and obtains bacillus liquid.
In the technical program, dry bacterium powder is mixed adjust being added in seawater with brown sugar, can make bacillus activation.
As preferably, the bacillus in bacillus liquid is bacillus subtilis or bacillus licheniformis.
As preferably, swimming crab Magna zoea larva Z 1phase throw something and feed Micro Algae and wheel animalcule, the daily ration, feeding quantity of Micro Algae is 50 × 10 4individual/mL, the daily ration, feeding quantity of wheel animalcule is 50-60/mL; Z 2phase-Z 4throw something and feed wheel animalcule and artemia nauplii of phase, the daily ration, feeding quantity of wheel animalcule is 50-60/mL, the feeding volume of artemia nauplii is 1.5-4/mL; Megalops larva and the young crab halogen worm adult of throwing something and feeding, the feeding volume of halogen worm adult is 20-30/mL, day throws something and feeds 4 times-8 times.
As preferably, at swimming crab Magna zoea larva Z 1phase-Z 4phase, water temperature is 20-26 DEG C, and at megalopa stage, water temperature is 24-26 DEG C, and blowing aeration, more than dissolved oxygen 5mg/L, and pH value 7.8-8.6, ammonia nitrogen is lower than 0.4mg/L, transparency 30cm-40cm.
As preferably, the concentration 10 for bait of throwing something and feeding in step c) 3-10 4the bacillus liquid of cfu/mL soaks 1-1.5h, and then throws something and feeds.
In the technical program, bait first soaks with bacillus liquid before throwing something and feeding, and can, by nutrient degraded in bait, make the young more abundant to absorbing of bait.
Beneficial effect of the present invention:
1) method of the present invention can purify and promote water quality, prevents the residual of conventional seedbed system method, does not change water in seedling raising process simultaneously, reduces growth cost;
2) the present invention can improve the survival rate that swimming crab is grown seedlings;
3) method of the present invention can improve the immunity of swimming crab body, adopts method of the present invention to avoid antibiotic etc abuse in production, reduces disease incident, improves production output.
 
Embodiment
Below in conjunction with specific embodiment, the present invention will be further explained:
Embodiment 1
The method that swimming crab is grown seedlings, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1 day, nursery pond is carried out disinfection with the bleaching powder of 50ppm, rinse well with clear water again, after nursery pond dries, the ooze through sterilization that is 4cm to nursery pond bottom laying depth, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria liquid, make Bdellovibrio content in seawater reach 10 3the content of cfu/mL, nitrifying bacteria reaches 10 3cfu/mL, then shading leaves standstill 5 days;
B) lay eggs: transfer grey black, idiosome heartbeat to and reach the 150 times/more than min female crab of fertilization to dropping into ovum look in the nursery pond of step a), the cloth seedling density of nursery pond is 15 × 10 4individual/m 3;
Wherein, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space is laid the thick fine sand of 10 cm, and depth of water is controlled at 0.6m, salinity 25 ‰, and female crab breeding density is 3/m 2.By 5% of the body weight shellfish meat of throwing something and feeding, remove in time residual bait; Female crab enters behind pond, stablizes 1 day under natural temperature, the accelerating that then starts to heat up, promotes production, and wintering pond water temperature improves 0.5 DEG C every day, to 18 DEG C of constant temperature; Female crab is cultured to female crab belly pieces of an egg and is black gray expandable in wintering pond;
Before the female crab input nursery pond of being fertilized is laid eggs, with 20mg/L formalin dipping bath 10min sterilization, clear water rinses;
C) post processing: after having laid eggs, female crab is pulled out, between nursery stage, the bait of regularly throwing something and feeding, threw in a bacillus liquid every 5 days, and in seawater, bacillus liquid concentration is 10 2cfu/mL.
Wherein, in step c), bacillus liquid is that dry bacterium powder (bacillus subtilis) and brown sugar are added in seawater by weight the ratio of 1:2, and activation 6h, then filters, and obtains bacillus liquid; Swimming crab Magna zoea larva Z 1phase throw something and feed Micro Algae and wheel animalcule, the daily ration, feeding quantity of Micro Algae is 50 × 10 4individual/mL, the daily ration, feeding quantity of wheel animalcule is 50/mL; Z 2phase-Z 4throw something and feed wheel animalcule and artemia nauplii of phase, the daily ration, feeding quantity of wheel animalcule is 50/mL, the feeding volume of artemia nauplii is 1.5/mL; Megalops larva and the young crab halogen worm adult of throwing something and feeding, the feeding volume of halogen worm adult is 20/mL, day throws something and feeds 4 times; Swimming crab Magna zoea larva Z 1phase-Z 420 DEG C of phase water temperatures, 24 DEG C of megalopa stage water temperatures, temperature difference per day ± 1 DEG C, and blowing aeration, dissolved oxygen 6mg/L, pH value 7.8, ammonia nitrogen 0.301mg/L, transparency 30cm; The concentration 10 for bait of throwing something and feeding in step c) 3the bacillus liquid of cfu/mL soaks 1h, and then throws something and feeds.
 
Embodiment 2
The method that swimming crab is grown seedlings, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1 day, nursery pond is carried out disinfection with the bleaching powder of 800ppm, rinse well with clear water again, after nursery pond dries, the ooze through sterilization that is 5cm to nursery pond bottom laying depth, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria, make Bdellovibrio content in seawater reach 10 4the content of cfu/mL, nitrifying bacteria reaches 10 4cfu/mL, then shading leaves standstill 6 days;
B) lay eggs: transfer grey black, idiosome heartbeat to and reach the 160 times/more than min female crab of fertilization to dropping into ovum look in the nursery pond of step a), the cloth seedling density of nursery pond is 18*10 4individual/m 3;
Wherein, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space is laid the thick fine sand of 11 cm, and depth of water is controlled at 0.8m, salinity 28 ‰, and female crab breeding density is 4/m 2.By 7% of the body weight clam worm of throwing something and feeding, remove in time residual bait; Female crab enters behind pond, stablizes 2 days under natural temperature, the accelerating that then starts to heat up, promotes production, and wintering pond water temperature improves 0.6 DEG C every day, to 19 DEG C of constant temperature; Female crab is cultured to female crab belly pieces of an egg and is black gray expandable in wintering pond;
Before female crab input nursery pond is laid eggs, with 80mg/L formalin dipping bath 11min sterilization, clear water rinses;
C) post processing: after having laid eggs, female crab is pulled out, between nursery stage, the bait of regularly throwing something and feeding, threw in a bacillus liquid every 6 days, and in seawater, bacillus liquid concentration is 10 4cfu/mL.
Wherein, in step c), bacillus liquid is that dry bacterium powder (bacillus licheniformis) and brown sugar are added in seawater by weight the ratio of 1:3, and activation 7h, then filters, and obtains bacillus liquid; Swimming crab Magna zoea larva Z 1phase throw something and feed Micro Algae and wheel animalcule, the daily ration, feeding quantity of Micro Algae is 50 × 10 4individual/mL, the daily ration, feeding quantity of wheel animalcule is 55/mL; Z 2phase-Z 4throw something and feed wheel animalcule and artemia nauplii of phase, the daily ration, feeding quantity of wheel animalcule is 55/mL, the feeding volume of artemia nauplii is 2/mL; Megalops larva and the young crab halogen worm adult of throwing something and feeding, the feeding volume of halogen worm adult is 25/mL, day throws something and feeds 6 times; Swimming crab Magna zoea larva Z 1phase-Z 422 DEG C of phases, 25 DEG C of megalopa stages, temperature difference per day ± 1 DEG C, and blowing aeration, dissolved oxygen 8mg/L, pH value 8.2, ammonia nitrogen 0.215mg/L, transparency 35cm; The concentration 10 for bait of throwing something and feeding in step c) 4the bacillus liquid of cfu/mL soaks 1.25h, and then throws something and feeds.
 
Embodiment 3
The method that swimming crab is grown seedlings, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 2 days, nursery pond is carried out disinfection with the bleaching powder of 100ppm, rinse well with clear water again, after nursery pond dries, the ooze through sterilization that is 8cm to nursery pond bottom laying depth, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria, make Bdellovibrio content in seawater reach 10 6the content of cfu/mL, nitrifying bacteria reaches 10 4cfu/mL, then shading leaves standstill 8 days;
B) lay eggs: transfer grey black, idiosome heartbeat to and reach the 160 times/more than min female crab of fertilization to dropping into ovum look in the nursery pond of step a), the cloth seedling density of nursery pond is 20*10 4individual/m 3;
Wherein, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space is laid the thick fine sand of 12 cm, and depth of water is controlled at 1m, salinity 30 ‰, and female crab breeding density is 5/m 2.By 8% of the body weight shellfish meat of throwing something and feeding, remove in time residual bait; Female crab enters behind pond, stablizes 2 days under natural temperature, the accelerating that then starts to heat up, promotes production, and wintering pond water temperature improves 0.8 DEG C every day, to 20 DEG C of constant temperature; Female crab is cultured to female crab belly pieces of an egg and is black gray expandable in wintering pond;
Before female crab input nursery pond is laid eggs, with 100mg/L formalin dipping bath 15min sterilization, clear water rinses;
C) post processing: after having laid eggs, female crab is pulled out, in seedling raising process, the bait of regularly throwing something and feeding, threw in a bacillus liquid every 7 days, and in seawater, bacillus liquid concentration is 10 5cfu/mL.
Wherein, in step c), bacillus liquid is that dry bacterium powder (bacillus subtilis) and brown sugar are added in seawater by weight the ratio of 1:4, and activation 8h, then filters, and obtains bacillus liquid; Swimming crab Magna zoea larva Z 1phase throw something and feed Micro Algae and wheel animalcule, the daily ration, feeding quantity of Micro Algae is 50 × 10 4individual/mL, the daily ration, feeding quantity of wheel animalcule is 60/mL; Z 2phase-Z 4throw something and feed wheel animalcule and artemia nauplii of phase, the daily ration, feeding quantity of wheel animalcule is 60/mL, the feeding volume of artemia nauplii is 4/mL; Megalops larva and the young crab halogen worm adult of throwing something and feeding, the feeding volume of halogen worm adult is 30/mL, day throws something and feeds 8 times; Swimming crab Magna zoea larva Z 1phase-Z 426 DEG C of phases, 26 DEG C of megalopa stages, temperature difference per day ± 1 DEG C, and blowing aeration, dissolved oxygen 9mg/L, pH value 8.6, ammonia nitrogen 0.105mg/L, transparency 40cm; The concentration 10 for bait of throwing something and feeding in step c) 4the bacillus liquid of cfu/mL soaks 1.5h, and then throws something and feeds.
Comparative example 1, is used the embodiment of the present invention 1 method therefor, but does not use Bdellovibrio.
Comparative example 2, is used the embodiment of the present invention 1 method therefor, but does not use nitrifying bacteria.
Comparative example 3, is used the embodiment of the present invention 1 method therefor, but does not use bacillus.
Swimming crab survival rate of seedling to embodiment 1-3 method therefor and comparative example 1-3 method therefor detects, and data are as follows:
The swimming crab survival rate of table 1, embodiment 1-3 method therefor and comparative example 1-3 method therefor
? Throw in the young/ten thousand tail Emerge/ten thousand tails Survival rate/%
Embodiment 1 15 10.7 71.33
Embodiment 2 18 12.5 69.44
Embodiment 3 20 15.7 78.5
Comparative example 1 15 6.8 45.33
Comparative example 2 15 7.6 50.6
Comparative example 3 15 7.1 47.33
As can be seen from the table, the survival rate of embodiment 1-3 method therefor is apparently higher than comparative example 1 method therefor, and comparative example 2 adds bacillus liquid in larval rearing water, in bait, soak with bacillus liquid, although its survival rate will be higher than comparative example 1 method therefor, but well below embodiment 1-3 method therefor, comparative example 3 adds nitrifying bacteria before the crab young is dropped in larval rearing water, although purified water quality, survival rate is still not high.
Embodiment 1-3 method therefor and comparative example 1-3 method therefor are carried out to water quality detection, substantially meet " water quality standard for fishery " (GB 11607-1989) standard, but the ammoniacal nitrogen of comparative example 1 and comparative example 2, nitrite nitrogen, sulphide measured value are all higher than embodiment 1-3 and comparative example 3, and concrete data are as follows:
Table 2, ammoniacal nitrogen, nitrite nitrogen, sulphide measured value
? Ammoniacal nitrogen (mg/L) Nitrite nitrogen (mg/L) Sulphide (mg/L)
Embodiment 1 0.301 0.013 0.113
Embodiment 2 0.215 0.011 0.112
Embodiment 3 0.105 0.012 0.109
Comparative example 1 0.512 0.019 0.129
Comparative example 2 0.499 0.018 0.124
Comparative example 3 0.311 0.011 0.111
As can be seen from Table 2, method therefor of the present invention can purify water.

Claims (9)

1. the method that swimming crab is grown seedlings, is characterized in that, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1-2 days, nursery pond is carried out disinfection with the bleaching powder of 50ppm-100ppm, rinse well with clear water again, after nursery pond dries, the ooze through sterilization that is 4-8cm to nursery pond bottom laying depth, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria liquid, make Bdellovibrio content in seawater reach 10 3-10 6the content of cfu/mL, nitrifying bacteria reaches 10 3-10 4cfu/mL, then shading leaves standstill 5-8 days;
B) lay eggs: transfer grey black, idiosome heartbeat to and reach the 150-160 time/more than min female crab being fertilized to dropping into ovum look in the nursery pond of step a), the cloth seedling density of nursery pond is 15-20 × 10 4individual/m 3;
C) post processing: after having laid eggs, female crab is pulled out, between nursery stage, the bait of regularly throwing something and feeding, threw in a bacillus liquid every 5-7 days, and in seawater, bacillus liquid concentration is 10 2-10 5cfu/mL.
2. the method that a kind of swimming crab according to claim 1 is grown seedlings, is characterized in that, the female crab before laying eggs is supported in wintering pond, 1/2 of pond floor space is laid the thick fine sand of 10-12 cm, depth of water is controlled at 0.6m-1m, salinity 25 ‰-30 ‰, and the breeding density of female crab is 3-5/m 2, by the 5%-8% of female crab body weight throw something and feed shellfish meat or clam worm.
3. the method that a kind of swimming crab according to claim 2 is grown seedlings, is characterized in that, supports when female crab, under natural temperature, stablizes 1-2 days, the accelerating that then starts to heat up, promotes production, and wintering pond water temperature improves 0.5-0.8 DEG C every day, to 18-20 DEG C of constant temperature.
4. the method that a kind of swimming crab according to claim 2 is grown seedlings, is characterized in that, before female crab input nursery pond is laid eggs, with the female crab 10-15min sterilization of 20mg/L-100mg/L formalin dipping bath, clear water rinses.
5. the method that a kind of swimming crab according to claim 1 is grown seedlings, is characterized in that, in step c), bacillus liquid is that dry bacterium powder and brown sugar are added in seawater by weight the ratio of 1:2-4, and activation 6-8h, then filters, and obtains bacillus liquid.
6. the method that a kind of swimming crab according to claim 1 is grown seedlings, is characterized in that, the bacillus in bacillus liquid is bacillus subtilis or bacillus licheniformis.
7. the method for growing seedlings according to a kind of swimming crab described in claim 1 or 2 or 3 or 4 or 5, is characterized in that, swimming crab Magna zoea larva Z 1phase throw something and feed Micro Algae and wheel animalcule, the daily ration, feeding quantity of Micro Algae is 50 × 10 4individual/mL, the daily ration, feeding quantity of wheel animalcule is 50-60/mL; Z 2phase-Z 4throw something and feed wheel animalcule and artemia nauplii of phase, the daily ration, feeding quantity of wheel animalcule is 50-60/mL, the feeding volume of artemia nauplii is 1.5-4/mL; Megalops larva and the young crab halogen worm adult of throwing something and feeding, the feeding volume of halogen worm adult is 20-30/mL, day throws something and feeds 4 times-8 times.
8. the method that a kind of swimming crab according to claim 7 is grown seedlings, is characterized in that, at swimming crab Magna zoea larva Z 1phase-Z 4phase, water temperature is 20-26 DEG C, and at megalopa stage, water temperature is 24-26 DEG C, and blowing aeration, more than dissolved oxygen 5mg/L, and pH value 7.8-8.6, ammonia nitrogen is lower than 0.4mg/L, transparency 30cm-40cm.
9. a kind of method that swimming crab is grown seedlings according to claim 1 or 5, is characterized in that, the concentration 10 for bait of throwing something and feeding in step c) 3-10 4the bacillus liquid of cfu/mL soaks 1-1.5h, and then throws something and feeds.
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CN107897072A (en) * 2017-11-15 2018-04-13 浙江海洋大学 A kind of P.sanguinolentus parent crab indoors artificial breeding method
CN111296336A (en) * 2020-04-09 2020-06-19 海南蓝泰邦生物技术有限公司 Method for cultivating sea urchin fries of white spine three rows

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