CN103843704B - A kind of method of swimming crab nursery - Google Patents
A kind of method of swimming crab nursery Download PDFInfo
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- CN103843704B CN103843704B CN201310741325.0A CN201310741325A CN103843704B CN 103843704 B CN103843704 B CN 103843704B CN 201310741325 A CN201310741325 A CN 201310741325A CN 103843704 B CN103843704 B CN 103843704B
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Abstract
The method of a kind of swimming crab nursery of the present invention, said method comprising the steps of: early-stage preparations: clear water soaked nursery pond after 1-2 days, with the bleaching powder of 50ppm-100ppm, nursery pond is carried out disinfection, the ooze through sterilization is laid bottom nursery pond, introduce seawater, add Bdellovibrio bacterium liquid and nitrifying bacteria; Lay eggs; Bacillus liquid is thrown in post processing seedling raising process.Method of the present invention can purify and promote water quality, prevents the residual of conventional seedbed system method, does not change water simultaneously, reduce growth cost in seedling raising process; The present invention can improve the survival rate of swimming crab nursery; Method of the present invention can improve the immunity of swimming crab body, and in adopting method Bi Miao of the present invention to produce, antibiotic etc abuse, reduces disease incident, improves production output.
Description
Technical field
The present invention relates to technical field of aquaculture, particularly relate to a kind of method of swimming crab nursery.
Background technology
Swimming crab, formal name used at school " decoy ", because carapace is shuttle shape, there are three projections in the central authorities of crust, so also known as Portunus trituberculatus Miers.Portunus trituberculatus Miers build is large, growth is fast, use value is high, delicious meat, nutritious, edible ratio are large, is not only of high nutritive value, but also has heat-clearing, the loose medical value such as blood and enriching yin.Mainly originate in the Huanghai Sea, the Bohai Sea and the East Sea.Due to the raising of people's living standard, marine products crab can not meet the growing demand of people, therefore starts artificial feeding.Portunus trituberculatus Miers tradition seedling raising process changes water in a large number, consumes mass energy, and increases production cost.Current natural waters is often polluted, seawater eutrophication, a large amount of bacterium, fungus breeding, brings very large impact to aquatic fry growing.Often change water in seedling raising process, the young, in order to shake down, needs energy i (in vivo) of beating, and reduces, which increase the cultivation time for the energy grown.The change of young living environment can make pathogenic microorganisms increase the threat of the young, just likely causes disease.In order to prevent and treat crab disease, many producers can use a large amount of agricultural chemicals, antibiotic and some high residue chemical synthetic drugs, what have is that pursuit growth rate and output even add chemical fertilizer and growth acceleration hormone in feed, thus crab Product quality and safety straight line is declined.
Chinese patent publication No. CN101755696A, date of publication on June 30th, 2010, name is called the method for Portunus trituberculatus Miers low latitudes season seeding and cultivation, this application case discloses a kind of method of Portunus trituberculatus Miers low latitudes season seeding and cultivation, in August to mid-October, from the natural marine site to the north of the Taiwan Straits or Portunus trituberculatus Miers culturing pool, obtain oogenesis Portunus trituberculatus Miers, ovigerous crab is transported to south (on the south the Taiwan Straits).With young crab as Portunus trituberculatus Miers seedling, carry out anti-season cultivation.The Portunus trituberculatus Miers of north cultivation can be cultivated in south.Its weak point is, changes water in a large number the immunity of swimming crab is reduced in seedling raising process, and cultivation survival rate reduces.
Summary of the invention
The object of the invention is to change water in a large number between conventional shuttle crab nursery stage to solve the immunity of swimming crab is reduced, the defect that cultivation survival rate reduces and a kind of method improving the swimming crab nursery of swimming crab juvenile crab immunity and survival rate is provided.
To achieve these goals, the present invention is by the following technical solutions:
A method for swimming crab nursery, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1-2 days, with the bleaching powder of 50ppm-100ppm, nursery pond is carried out disinfection, rinse well with clear water again, after nursery pond dries, bottom nursery pond, laying depth is the ooze through sterilization of 4-8cm, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria liquid, make Bdellovibrio content in seawater reach 10
3-10
6the content of cfu/mL, nitrifying bacteria reaches 10
3-10
4cfu/mL, then shading leaves standstill 5-8 days;
B) lay eggs: in the nursery pond of step a), drop into the female crab be fertilized that ovum look transfers grey black to, idiosome heartbeat reaches 150-160 time/more than min, the cloth seedling density of nursery pond is 15-20 × 10
4individual/m
3;
C) post processing: after having laid eggs, pulls out female crab, and between nursery stage, bait of regularly throwing something and feeding, threw in a bacillus liquid every 5-7 days, in seawater, bacillus liquid concentration is 10
2-10
5cfu/mL.
In the technical program, in larval rearing water, nitrifying bacteria liquid is added in step a), nitrifying bacteria is the bacterium that mineralized nitrogen poisonous in water can be become nontoxic nitric acid state, and in the water of nursery pond, add nitrifying bacteria liquid, nitrifying bacteria can be made first to purify seawater, the poisonous mineralized nitrogen in seawater is become the materials such as nitric acid state;
Bdellovibrio is arc gram-Negative bacillus, and extensively distribution is with in waters soil and animal body, size 0.2-0.25 μm × 0.5-1.4 μm, extremely single flagellum, and motion is active.Bdellovibrio is a kind of bacterium parasitizing bacterium, can invest on the cell wall of host bacterium, and promptly pierce in host cell with the absorber of self, utilizes the nourishing and growing of host, breeds, finally cause the cracking of host bacterium.Bdellovibrio is selective to host, specially with Gram-negative bacterias such as vibrios, pseudomonad, Aeromonas, tardas for host, stronger to the cracking ability of pathogenic bacteria, add Bdellovibrio and can prevent growing the pathogenetic bacteria that crab is harmful to, effectively to avoid in swimming crab seedling raising process pathogenetic bacteria to the threat of the swimming crab young;
Step c) adds bacillus liquid in larval rearing water, bacillus can be bacillus licheniformis also can be bacillus subtilis, organic matter decomposition power is strong, can produce that abundant metabolism product, antibacterial, harmful power of going out are strong, the function of deodorizing, in seedling raising process, the ight soil that the young in each period produces can be decomposed by bacillus, avoids the pollution of water quality, the growth to crab young immune organ can be stimulated, strengthen immunity of organisms.
As preferably, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space lays the thick fine sand of 10-12cm, and depth of water controls at 0.6m-1m, salinity 25 ‰-30 ‰, female crab breeding density be 3-5 only/m
2, to throw something and feed shellfish meat or clam worm by the 5%-8% of body weight.
As preferably, when supporting female crab, under natural temperature, stablize 1-2 days, then start conditionting culture, promote production, wintering pond water temperature improves 0.5-0.8 DEG C every day, to 18-20 DEG C of constant temperature.
As preferably, before female crab input nursery pond is laid eggs, sterilize with 20mg/L-100mg/L formalin dipping bath 10-15min, clear water flushing.
As preferably, in step c), bacillus liquid adds in seawater by dry bacterium powder and brown sugar by weight the ratio of 1:2-4, and activation 6-8h, then filters, obtain bacillus liquid.
In the technical program, dry bacterium powder is added in seawater with brown sugar mixing tune, bacillus can be made to activate.
As preferably, the bacillus in bacillus liquid is bacillus subtilis or bacillus licheniformis.
As preferably, swimming crab Magna zoea larva Z
1phase throws something and feeds Micro Algae and wheel animalcule, and the daily ration, feeding quantity of Micro Algae is 50 × 10
4individual/mL, the daily ration, feeding quantity of wheel animalcule is 50-60/mL; Z
2phase-Z
4phase throws something and feeds wheel animalcule and artemia nauplii, and the daily ration, feeding quantity of wheel animalcule is 50-60/mL, and the feeding volume of artemia nauplii is 1.5-4/mL; Megalops larva and young crab are thrown something and fed halogen worm adult, and the feeding volume of halogen worm adult is 20-30/mL, throws something and feeds day 4 times-8 times.
As preferably, at swimming crab Magna zoea larva Z
1phase-Z
4phase, water temperature is 20-26 DEG C, and at megalopa stage, water temperature is 24-26 DEG C, and blowing aeration, more than dissolved oxygen 5mg/L, pH value 7.8-8.6, ammonia nitrogen lower than 0.4mg/L, transparency 30cm-40cm.
As preferably, the bait concentration 10 of throwing something and feeding in step c)
3-10
4the bacillus liquid of cfu/mL soaks 1-1.5h, and then throws something and feeds.
In the technical program, bait first soaks with bacillus liquid before throwing something and feeding, and can, by the degraded of bait Middle nutrition element, make the young more abundant to absorbing of bait.
Beneficial effect of the present invention:
1) method of the present invention can purify and promote water quality, prevents the residual of conventional seedbed system method, does not change water simultaneously, reduce growth cost in seedling raising process;
2) the present invention can improve the survival rate of swimming crab nursery;
3) method of the present invention can improve the immunity of swimming crab body, adopts and present method avoids antibiotic etc abuse in production, reduce disease incident, improve production output.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further explained:
Embodiment 1
A method for swimming crab nursery, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1 day, with the bleaching powder of 50ppm, nursery pond is carried out disinfection, rinse well with clear water again, after nursery pond dries, bottom nursery pond, laying depth is the ooze through sterilization of 4cm, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria liquid, make Bdellovibrio content in seawater reach 10
3the content of cfu/mL, nitrifying bacteria reaches 10
3cfu/mL, then shading leaves standstill 5 days;
B) lay eggs: in the nursery pond of step a), drop into the female crab of fertilization that ovum look transfers grey black to, idiosome heartbeat reaches 150 times/more than min, the cloth seedling density of nursery pond is 15 × 10
4individual/m
3;
Wherein, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space lays the thick fine sand of 10cm, and depth of water controls at 0.6m, salinity 25 ‰, and female crab breeding density is 3/m
2.To throw something and feed shellfish meat by 5% of body weight, remove residual bait in time; After female crab enters pond, stablize 1 day under natural temperature, then start conditionting culture, promote production, wintering pond water temperature improves 0.5 DEG C every day, to 18 DEG C of constant temperature; It is black gray expandable that female crab is cultured to female crab belly pieces of an egg in wintering pond;
Before female crab input nursery pond of being fertilized is laid eggs, sterilize with 20mg/L formalin dipping bath 10min, clear water rinses;
C) post processing: after having laid eggs, pulls out female crab, and between nursery stage, bait of regularly throwing something and feeding, threw in a bacillus liquid every 5 days, in seawater, bacillus liquid concentration is 10
2cfu/mL.
Wherein, in step c), bacillus liquid adds in seawater by dry bacterium powder (bacillus subtilis) and brown sugar by weight the ratio of 1:2, and activation 6h, then filters, obtain bacillus liquid; Swimming crab Magna zoea larva Z
1phase throws something and feeds Micro Algae and wheel animalcule, and the daily ration, feeding quantity of Micro Algae is 50 × 10
4individual/mL, the daily ration, feeding quantity of wheel animalcule is 50/mL; Z
2phase-Z
4phase throws something and feeds wheel animalcule and artemia nauplii, and the daily ration, feeding quantity of wheel animalcule is 50/mL, and the feeding volume of artemia nauplii is 1.5/mL; Megalops larva and young crab are thrown something and fed halogen worm adult, and the feeding volume of halogen worm adult is 20/mL, throws something and feeds day 4 times; Swimming crab Magna zoea larva Z
1phase-Z
4phase water temperature 20 DEG C, megalopa stage water temperature 24 DEG C, temperature difference per day ± 1 DEG C, and blowing aeration, dissolved oxygen 6mg/L, pH value 7.8, ammonia nitrogen 0.301mg/L, transparency 30cm; The bait concentration 10 of throwing something and feeding in step c)
3the bacillus liquid of cfu/mL soaks 1h, and then throws something and feeds.
Embodiment 2
A method for swimming crab nursery, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1 day, with the bleaching powder of 800ppm, nursery pond is carried out disinfection, rinse well with clear water again, after nursery pond dries, bottom nursery pond, laying depth is the ooze through sterilization of 5cm, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria, make Bdellovibrio content in seawater reach 10
4the content of cfu/mL, nitrifying bacteria reaches 10
4cfu/mL, then shading leaves standstill 6 days;
B) lay eggs: in the nursery pond of step a), drop into the female crab of fertilization that ovum look transfers grey black to, idiosome heartbeat reaches 160 times/more than min, the cloth seedling density of nursery pond is 18*10
4individual/m
3;
Wherein, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space lays the thick fine sand of 11cm, and depth of water controls at 0.8m, salinity 28 ‰, and female crab breeding density is 4/m
2.To throw something and feed clam worm by 7% of body weight, remove residual bait in time; After female crab enters pond, stablize 2 days under natural temperature, then start conditionting culture, promote production, wintering pond water temperature improves 0.6 DEG C every day, to 19 DEG C of constant temperature; It is black gray expandable that female crab is cultured to female crab belly pieces of an egg in wintering pond;
Before female crab input nursery pond is laid eggs, sterilize with 80mg/L formalin dipping bath 11min, clear water rinses;
C) post processing: after having laid eggs, pulls out female crab, and between nursery stage, bait of regularly throwing something and feeding, threw in a bacillus liquid every 6 days, in seawater, bacillus liquid concentration is 10
4cfu/mL.
Wherein, in step c), bacillus liquid adds in seawater by dry bacterium powder (bacillus licheniformis) and brown sugar by weight the ratio of 1:3, and activation 7h, then filters, obtain bacillus liquid; Swimming crab Magna zoea larva Z
1phase throws something and feeds Micro Algae and wheel animalcule, and the daily ration, feeding quantity of Micro Algae is 50 × 10
4individual/mL, the daily ration, feeding quantity of wheel animalcule is 55/mL; Z
2phase-Z
4phase throws something and feeds wheel animalcule and artemia nauplii, and the daily ration, feeding quantity of wheel animalcule is 55/mL, and the feeding volume of artemia nauplii is 2/mL; Megalops larva and young crab are thrown something and fed halogen worm adult, and the feeding volume of halogen worm adult is 25/mL, throws something and feeds day 6 times; Swimming crab Magna zoea larva Z
1phase-Z
4phase 22 DEG C, megalopa stage 25 DEG C, temperature difference per day ± 1 DEG C, and blowing aeration, dissolved oxygen 8mg/L, pH value 8.2, ammonia nitrogen 0.215mg/L, transparency 35cm; The bait concentration 10 of throwing something and feeding in step c)
4the bacillus liquid of cfu/mL soaks 1.25h, and then throws something and feeds.
Embodiment 3
A method for swimming crab nursery, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 2 days, with the bleaching powder of 100ppm, nursery pond is carried out disinfection, rinse well with clear water again, after nursery pond dries, bottom nursery pond, laying depth is the ooze through sterilization of 8cm, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria, make Bdellovibrio content in seawater reach 10
6the content of cfu/mL, nitrifying bacteria reaches 10
4cfu/mL, then shading leaves standstill 8 days;
B) lay eggs: in the nursery pond of step a), drop into the female crab of fertilization that ovum look transfers grey black to, idiosome heartbeat reaches 160 times/more than min, the cloth seedling density of nursery pond is 20*10
4individual/m
3;
Wherein, the female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space lays the thick fine sand of 12cm, and depth of water controls at 1m, salinity 30 ‰, and female crab breeding density is 5/m
2.To throw something and feed shellfish meat by 8% of body weight, remove residual bait in time; After female crab enters pond, stablize 2 days under natural temperature, then start conditionting culture, promote production, wintering pond water temperature improves 0.8 DEG C every day, to 20 DEG C of constant temperature; It is black gray expandable that female crab is cultured to female crab belly pieces of an egg in wintering pond;
Before female crab input nursery pond is laid eggs, sterilize with 100mg/L formalin dipping bath 15min, clear water rinses;
C) post processing: after having laid eggs, pulls out female crab, and in seedling raising process, bait of regularly throwing something and feeding, threw in a bacillus liquid every 7 days, in seawater, bacillus liquid concentration is 10
5cfu/mL.
Wherein, in step c), bacillus liquid adds in seawater by dry bacterium powder (bacillus subtilis) and brown sugar by weight the ratio of 1:4, and activation 8h, then filters, obtain bacillus liquid; Swimming crab Magna zoea larva Z
1phase throws something and feeds Micro Algae and wheel animalcule, and the daily ration, feeding quantity of Micro Algae is 50 × 10
4individual/mL, the daily ration, feeding quantity of wheel animalcule is 60/mL; Z
2phase-Z
4phase throws something and feeds wheel animalcule and artemia nauplii, and the daily ration, feeding quantity of wheel animalcule is 60/mL, and the feeding volume of artemia nauplii is 4/mL; Megalops larva and young crab are thrown something and fed halogen worm adult, and the feeding volume of halogen worm adult is 30/mL, throws something and feeds day 8 times; Swimming crab Magna zoea larva Z
1phase-Z
4phase 26 DEG C, megalopa stage 26 DEG C, temperature difference per day ± 1 DEG C, and blowing aeration, dissolved oxygen 9mg/L, pH value 8.6, ammonia nitrogen 0.105mg/L, transparency 40cm; The bait concentration 10 of throwing something and feeding in step c)
4the bacillus liquid of cfu/mL soaks 1.5h, and then throws something and feeds.
Comparative example 1, uses the embodiment of the present invention 1 method therefor, but does not use Bdellovibrio.
Comparative example 2, uses the embodiment of the present invention 1 method therefor, but does not use nitrifying bacteria.
Comparative example 3, uses the embodiment of the present invention 1 method therefor, but does not use bacillus.
Detect the swimming crab survival rate of seedling of embodiment 1-3 method therefor and comparative example 1-3 method therefor, data are as follows:
The swimming crab survival rate of table 1, embodiment 1-3 method therefor and comparative example 1-3 method therefor
Throw in the young/ten thousand tail | To emerge/ten thousand tails | Survival rate/% | |
Embodiment 1 | 15 | 10.7 | 71.33 |
Embodiment 2 | 18 | 12.5 | 69.44 |
Embodiment 3 | 20 | 15.7 | 78.5 |
Comparative example 1 | 15 | 6.8 | 45.33 |
Comparative example 2 | 15 | 7.6 | 50.6 |
Comparative example 3 | 15 | 7.1 | 47.33 |
As can be seen from the table, the survival rate of embodiment 1-3 method therefor is apparently higher than comparative example 1 method therefor, and comparative example 2 adds bacillus liquid in larval rearing water, soak with bacillus liquid in bait, although its survival rate is higher than comparative example 1 method therefor, but well below embodiment 1-3 method therefor, comparative example 3 adds nitrifying bacteria before the crab young is dropped in larval rearing water, although purified water quality, survival rate is still not high.
Water quality detection is carried out to embodiment 1-3 method therefor and comparative example 1-3 method therefor, substantially " water quality standard for fishery " (GB11607-1989) standard is met, but the ammoniacal nitrogen of comparative example 1 and comparative example 2, nitrite nitrogen, sulphide measured value are all higher than embodiment 1-3 and comparative example 3, and concrete data are as follows:
Table 2, ammoniacal nitrogen, nitrite nitrogen, sulphide measured value
Ammoniacal nitrogen (mg/L) | Nitrite nitrogen (mg/L) | Sulphide (mg/L) | |
Embodiment 1 | 0.301 | 0.013 | 0.113 |
Embodiment 2 | 0.215 | 0.011 | 0.112 |
Embodiment 3 | 0.105 | 0.012 | 0.109 |
Comparative example 1 | 0.512 | 0.019 | 0.129 |
Comparative example 2 | 0.499 | 0.018 | 0.124 |
Comparative example 3 | 0.311 | 0.011 | 0.111 |
As can be seen from Table 2, method therefor of the present invention can purify water.
Claims (5)
1. a method for swimming crab nursery, is characterized in that, said method comprising the steps of:
A) early-stage preparations: clear water soaked nursery pond after 1-2 days, with the bleaching powder of 50ppm-100ppm, nursery pond is carried out disinfection, rinse well with clear water again, after nursery pond dries, bottom nursery pond, laying depth is the ooze through sterilization of 4-8cm, then in nursery pond, introduce seawater, in nursery pond, add Bdellovibrio bacterium liquid and nitrifying bacteria liquid, make Bdellovibrio content in seawater reach 10
3-10
6the content of cfu/mL, nitrifying bacteria reaches 10
3-10
4cfu/mL, then shading leaves standstill 5-8 days;
B) lay eggs: in the nursery pond of step a), drop into the female crab be fertilized that ovum look transfers grey black to, idiosome heartbeat reaches 150-160 time/more than min, the cloth seedling density of nursery pond is 15-20 × 10
4individual/m
3;
C) post processing: after having laid eggs, pulls out female crab, and between nursery stage, bait of regularly throwing something and feeding, threw in a bacillus liquid every 5-7 days, in seawater, bacillus liquid concentration is 10
2-10
5cfu/mL; The bait concentration 10 of throwing something and feeding in step c)
3-10
4the bacillus liquid of cfu/mL soaks 1-1.5h, and then throws something and feeds; Female crab before laying eggs is supported in wintering pond, and 1/2 of pond floor space lays the thick fine sand of 10-12cm, and depth of water controls at 0.6m-1m, salinity 25 ‰-30 ‰, and the breeding density of female crab is 3-5/m
2, to throw something and feed shellfish meat or clam worm by the 5%-8% of female crab body weight; In step c), bacillus liquid adds in seawater by dry bacterium powder and brown sugar by weight the ratio of 1:2-4, and activation 6-8h, then filters, obtain bacillus liquid; Swimming crab Magna zoea larva Z
1phase throws something and feeds Micro Algae and wheel animalcule, and the daily ration, feeding quantity of Micro Algae is 50 × 10
4individual/mL, the daily ration, feeding quantity of wheel animalcule is 50-60/mL; Z
2phase-Z
4phase throws something and feeds wheel animalcule and artemia nauplii, and the daily ration, feeding quantity of wheel animalcule is 50-60/mL, and the feeding volume of artemia nauplii is 1.5-4/mL; Megalops larva and young crab are thrown something and fed halogen worm adult, and the feeding volume of halogen worm adult is 20-30/mL, throws something and feeds day 4 times-8 times.
2. the method for a kind of swimming crab nursery according to claim 1, is characterized in that, when supporting female crab, under natural temperature, stablize 1-2 days, then start conditionting culture, promote production, wintering pond water temperature improves 0.5-0.8 DEG C every day, to 18-20 DEG C of constant temperature.
3. the method for a kind of swimming crab nursery according to claim 1, is characterized in that, before female crab input nursery pond is laid eggs, sterilize with the female crab 10-15min of 20mg/L-100mg/L formalin dipping bath, clear water rinses.
4. the method for a kind of swimming crab nursery according to claim 1, is characterized in that, the bacillus in bacillus liquid is bacillus subtilis or bacillus licheniformis.
5. the method for a kind of swimming crab nursery according to claim 1, is characterized in that, at swimming crab Magna zoea larva Z
1phase-Z
4phase, water temperature is 20-26 DEG C, and at megalopa stage, water temperature is 24-26 DEG C, and blowing aeration, more than dissolved oxygen 5mg/L, pH value 7.8-8.6, ammonia nitrogen lower than 0.4mg/L, transparency 30cm-40cm.
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