CN108541814A - A kind of prevention Penaeus Vannmei just additive for microbe feedstuff and preparation method thereof of syndrome in vain - Google Patents
A kind of prevention Penaeus Vannmei just additive for microbe feedstuff and preparation method thereof of syndrome in vain Download PDFInfo
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- CN108541814A CN108541814A CN201810279362.7A CN201810279362A CN108541814A CN 108541814 A CN108541814 A CN 108541814A CN 201810279362 A CN201810279362 A CN 201810279362A CN 108541814 A CN108541814 A CN 108541814A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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Abstract
The invention discloses a kind of prevention Penaeus Vannmei just additive for microbe feedstuff and preparation method thereof of syndrome in vain.Wherein, additive for microbe feedstuff presses the material composition of following mass ratio：46 parts of Bdellovibrio microcapsules, 46 parts of antibacterial bacillus amyloliquefaciens microcapsules；A concentration of the 3.69 × 10 of the bacteriophagic Bdellovibrio microcapsules7Pfu/g~3.70 × 107Pfu/g, a concentration of the 1.91 × 10 of the antibacterial bacillus amyloliquefaciens microcapsules9cfu/g.This additive for microbe feedstuff, which has, prevents the Penaeus Vannmei just remarkable result of syndrome in vain, and the additive for microbe feedstuff is used for the Penaeus Vannmei just prevention of syndrome in vain, safe without toxic side effect, pathogen will not be caused to generate drug resistance and medicament residue, water quality and aquatic products pollution as chemicals, operation is easier, dosage is lower, and use cost is lower, is more advantageous to the development of Penaeus Vannmei healthy aquaculture and increases with cultured output.
Description
Technical field
The present invention relates to the just microorganism feedings of syndrome in vain of prawn disease prophylactic agent more particularly to a kind of prevention Penaeus Vannmei
Feed additives and preparation method thereof.
Background technology
In recent years, there is Penaeus Vannmei and just integrate in vain in various degree in the provinces and cities such as China Guangxi, Guangdong, Fujian, Zhejiang
Sign, and be in spreading trend year by year, hazard ratio is more serious.Just the cardinal symptom of syndrome is Penaeus Vannmei in vain：Illness South America is white
The excrement of prawn is elongated white (such as cotton thread), has stickiness, keeps afloat, and a large amount of foul smellings of assembling distribute (Penaeus Vannmei sky
The prevention science of intestines in vain just is breeded fish, and 2013,2：62-62.).Currently, just syndrome mainly uses En Nuosha to Penaeus Vannmei in vain
Magnitude antibiotic (just the separation of syndrome cause of disease comma bacillus is in progress Penaeus Vannmei with drug sensitive test animal medicines in vain,
2016,37 (2)：128-132.) or joint photosynthetic bacteria+calcium oxide+Sanhuang San+Ofloxacin+sodium bicarbonate etc. some drugs are matched
Conjunction treatment (the prevention science of Penaeus Vannmei jejunum in vain just is breeded fish, and 2013,2：The drawing of 62-62. Penaeus Vannmeis just treats one in vain
Example present age aquatic products, 2011,10：38-38.), or using compound formulation (highly concentrated bacteriophagic Bdellovibrio original powder+highly concentrated compound gemma bar
Bacterium original powder+vitamin C original powder) (compound formulation for the treatment of Penaeus Vannmei white excrement disease and preparation method thereof number of patent application：
201310638319.2) it is treated, but these treatment preparations are used to the Penaeus Vannmei just treatment of syndrome in vain, belong to south
Use after the onset of penaeus vannamei, using or preparation process is extremely complex or the entire course for the treatment of relates at least five kinds of above drugs,
Production operation is complicated, and dosage is larger, higher operating costs, to increase raiser to the control difficulty of the disease and
Cost.Since just syndrome has morbidity anxious to Penaeus Vannmei in vain, the features such as big is endangered, once morbidity, is such as not treated in time, just
It will appear massive mortality, be unable to control.Therefore, exploitation prevent Penaeus Vannmei in vain just the feed addictive of syndrome relative to
It is even more important for its treatment preparation, but so far without the prevention preparation of any disease.In view of this, the present invention is prepared for one kind
Prevent the Penaeus Vannmei just additive for microbe feedstuff of syndrome in vain, has filled up and prevented the Penaeus Vannmei just production of syndrome in vain
Product blank.
Invention content
For this purpose, technical problem to be solved by the invention is to provide a kind of prevention Penaeus Vannmei just micro- lifes of syndrome in vain
Object feed addictive, overcomes the shortcomings of the prior art.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
A kind of prevention Penaeus Vannmei just additive for microbe feedstuff of syndrome in vain, it is characterised in that press following mass ratio
Material composition：4-6 parts of bacteriophagic Bdellovibrio microcapsules, 4-6 parts of antibacterial bacillus amyloliquefaciens microcapsules；The bacteriophagic Bdellovibrio
A concentration of the 3.69 × 10 of microcapsules7Pfu/g~3.70 × 107Pfu/g, the antibacterial bacillus amyloliquefaciens microcapsules it is dense
Degree is 1.91 × 109cfu/g。
In a preferred embodiment of the invention, 5 parts of the bacteriophagic Bdellovibrio microcapsules, the antibacterial bacillus amyloliquefaciens
5 parts of microcapsules.
The bacteriophagic Bdellovibrio microcapsules used in the present invention, in the patent text of Patent No. ZL201210383061.1
Antibacterial bacillus amyloliquefaciens microcapsules on the books in offering, using, in the patent text of Patent No. ZL201210297950.6
It is on the books in offering.
In addition, the present invention also provides the preparation method of mentioned microorganism feed addictive, which is：
Bacteriophagic Bdellovibrio microcapsules and antibacterial bacillus amyloliquefaciens microcapsules are weighed by above-mentioned mass ratio；First by Bdellovibrio
Microcapsules and antibacterial bacillus amyloliquefaciens microcapsules uniform stirring under the conditions of 15-25 DEG C mix, mixing speed 100-150r/
Min, mixing time 0.5-1.0h are entirely stirred process and are in air-tight state.
Above-mentioned technical proposal is used, the experiment proved that, additive for microbe feedstuff of the invention has prevention South America right in vain
The shrimp just remarkable result of syndrome, and the additive for microbe feedstuff safe without toxic side effect in vain, will not be as chemicals one
Sample causes pathogen to generate drug resistance and medicament residue, and can overcome reported prevention Penaeus Vannmei just syndrome in vain
Need the drug partner treatment more than 5 kinds such as photosynthetic bacteria+calcium oxide+Sanhuang San+Ofloxacin+sodium bicarbonate that can just have effect
Problem, with the Penaeus Vannmei of existing report just medicine (the highly concentrated bacteriophagic Bdellovibrio original powder+highly concentrated compound bud of syndrome in vain
Spore bacillus original powder+vitamin C original powder) (compound formulation for the treatment of Penaeus Vannmei white excrement disease and preparation method thereof number of patent application：
201310638319.2) it compares, operation is easier, and dosage is lower, and use cost is lower, is more advantageous to Penaeus Vannmei health and supports
Development is grown with cultured output to increase.
Specific implementation mode
Materials and methods
1.1 experiment material
Bacteriophagic Bdellovibrio microcapsules, concentration (i.e. bacterial content) are 3.69 × 107Pfu/g~3.70 × 107Pfu/g (phagocytosis leech
The preparation method patent No. of vibrios microcapsules：ZL201210383061.1), provided by Shanghai Ocean University.
Antibacterial bacillus amyloliquefaciens microcapsules, concentration (i.e. bacterial content) are 1.91 × 109Cfu/g (antibacterial solution starch buds
The preparation method patent No. of spore bacillus microcapsules：ZL201210297950.6), provided by Shanghai Ocean University.
White just syndrome virus opportunistic pathogen comma bacillus bacteria suspension, is provided by Shanghai Ocean University's aquatic animal cause of disease library.
Litopenaeus vannamei compound feed is purchased from Guangdong sea Rong Chuan feed corporation,Ltds of large group.
Healthy Penaeus Vannmei, totally 300 tail, is provided by Cixi Long Wu fishery professionals cooperative society.
Enrofloxacin original powder, content are more than 99.7%, are purchased from Zhejiang Province Guobang Pharmaceutical Co., Ltd.
Treat compound formulation (the preparation side of the compound formulation for the treatment of Penaeus Vannmei white excrement disease of Penaeus Vannmei white excrement disease
Method number of patent application：201310638319.2), it is purchased from Shanghai Lvao Biotechnology Co., Ltd..
1.2 prevent the Penaeus Vannmei just preparation of the additive for microbe feedstuff of syndrome in vain
By bacteriophagic Bdellovibrio microcapsules, antibacterial bacillus amyloliquefaciens microcapsules in mass ratio 5：5 uniform stirrings mix, i.e.,
To prevent the Penaeus Vannmei just additive for microbe feedstuff of syndrome in vain.Concrete operations are：First by 50 kilograms of antibacterial solution starch
Bacillus microcapsules are stirred with 50 kilograms of bacteriophagic Bdellovibrio uniform microcapsules, are stirred process and are in air-tight state,
15-25 DEG C of temperature, mixing speed 100-150r/min, mixing time 0.5-1.0h.
The selection of 1.3 healthy Penaeus Vannmeis
Selection vigor is good, disease-free wound, healthy Penaeus Vannmei physical strong, of uniform size, and it is 60L's to cultivate in capacity
In glass aquarium, 30 tail of each aquarium carries out preventing the Penaeus Vannmei just additive for microbe feedstuff pair of syndrome in vain
The Penaeus Vannmei just experiment of preventive effects of syndrome in vain.
1.4 prevent the Penaeus Vannmeis just application effect of the additive for microbe feedstuff of syndrome in vain
Before experiment, bacterial bait and bacterium medicine mixing bait are prepared respectively.I.e. by a concentration of 2.0 × 108The white just syndrome of cfu/mL
Pathogen comma bacillus bacteria suspension, which is uniformly sprayed in litopenaeus vannamei compound feed, is formed with toadstool bait, makes toxic bacterial bait suddenly
Random vibrios content is 1.0 × 108Cfu/g feeds；By a concentration of 2.0 × 108The white just syndrome virus opportunistic pathogen comma bacillus of cfu/mL
Bacteria suspension, which is uniformly sprayed in litopenaeus vannamei compound feed, is formed with toadstool bait, then Enrofloxacin original powder and will have toadstool again
Bait has uniformly been mixed and made into toadstool-Enrofloxacin mixing bait, makes the content for having comma bacillus in toadstool-Enrofloxacin mixing bait be
1.0×108The whole dosage of cfu/g feeds, Enrofloxacin is 1.0g/Kg feeds；By a concentration of 2.0 × 108Cfu/mL's is white just comprehensive
Simulator sickness pathogen comma bacillus bacteria suspension is uniformly sprayed in litopenaeus vannamei compound feed, then will treat Penaeus Vannmei again
The compound formulation of white excrement disease is uniformly mixed and made into toadstool-treatment Penaeus Vannmei white excrement disease with litopenaeus vannamei compound feed
Compound formulation mixing bait makes the content of comma bacillus in the compound formulation mixing bait for having toadstool-treatment Penaeus Vannmei white excrement disease
It is 1.0 × 108Cfu/g feeds, the whole dosage for treating the compound formulation of Penaeus Vannmei white excrement disease are 5.0g/Kg feeds；It will be dense
Degree is 2.0 × 108The white just syndrome virus opportunistic pathogen comma bacillus bacteria suspension of cfu/mL is uniformly sprayed at Penaeus Vannmei cooperation and raises
On material, then preventing Penaeus Vannmei again, just the additive for microbe feedstuff of syndrome and litopenaeus vannamei compound feed are equal in vain
It is even to be mixed and made into toadstool-additive for microbe feedstuff mixing bait, make have cholera in toadstool-additive for microbe feedstuff mixing bait
The content of vibrios is 1.0 × 108Cfu/g feeds prevent Penaeus Vannmei just end of the additive for microbe feedstuff of syndrome in vain
Dosage is respectively 1.0g/Kg feeds, 2.0g/Kg feeds, 3.0g/Kg feeds.
1 negative control group of setting, 2 positive controls, 1 low dosage test group, 1 middle dosage test group and 1
High dose test group, each test group are put into 30 tail of healthy Penaeus Vannmei.Negative control group feeds Penaeus Vannmei daily to be had
Toadstool bait, feeding volume are the 2% of shrimp total weight；Positive controls 1 feed Penaeus Vannmei daily toadstool-Enrofloxacin mixing
Bait, feeding volume are the 2% of shrimp total weight；Positive controls 2 feed Penaeus Vannmei daily has toadstool-treatment Penaeus Vannmei white
Just sick compound formulation mixing bait, feeding volume are the 2% of shrimp total weight；Low dosage test group feeds Penaeus Vannmei and contains daily
1.0g/Kg feeds prevent Penaeus Vannmei, and just the additive for microbe feedstuff of syndrome has an addition of toadstool-microbiological feed in vain
Agent mixing bait, feeding volume are the 2% of shrimp total weight；It is right in vain that middle dosage test group feeds the prevention of feed containing 2.0g/Kg South America daily
Just the have toadstool-additive for microbe feedstuff mixing bait, feeding volume of the additive for microbe feedstuff of syndrome are that shrimp is overall to shrimp in vain
The 2% of weight；High dose test group feeds Penaeus Vannmei feed containing 3.0mg/Kg and prevents Penaeus Vannmei just syndrome in vain daily
Additive for microbe feedstuff have toadstool-additive for microbe feedstuff mixing bait, feeding volume is the 2% of shrimp total weight.Each
Group (negative control group, positive controls, low dosage test group, middle dosage test group, high dose test group) is continuously fed
18d, the mortality of every 3 days statistics each group Penaeus Vannmeis, calculates the death rate and Effective Vate of Protection during experiment.During experiment
Continuous aeration does not change water, and water temperature control is being 28 DEG C.
2. experimental result
Experimental result be shown in Table 1 (prevent Penaeus Vannmei in vain just the additive for microbe feedstuff of syndrome to Penaeus Vannmei
The preventive effect of white just syndrome).The experimental results showed that the Penaeus Vannmei in negative control group and positive controls 1 occurs
, there is the higher death rate in typical white just Syndrome Symptoms, and are added to respectively in litopenaeus vannamei compound feed
Treat the compound formulation of Penaeus Vannmei white excrement disease and the prevention south of 1.0g/Kg feeds, 2.0g/Kg feeds, 3.0g/Kg feeds
Just the additive for microbe feedstuff of syndrome can substantially reduce the Penaeus Vannmei just morbidity of syndrome in vain to penaeus vannamei in vain
Thus rate reduces the death rate of Penaeus Vannmei caused by white just syndrome：Continuous to feed 18d, feeding volume is shrimp total weight
The prevention Penaeus Vannmei just microorganism of syndrome in vain of 2%, 1.0g/Kg feed, 2.0g/Kg feeds, 3.0g/Kg feed dosage
Feed addictive makes Penaeus Vannmei death rate caused by white just syndrome reduce 30% compared with negative control group respectively,
76.67%, the compound formulation of the treatment Penaeus Vannmei white excrement disease of 76.67%, 5g/Kg feeds dosage makes Penaeus Vannmei because white
Just the death rate caused by syndrome reduces 52.17%, especially 2.0g/Kg feeds, 3.0g/Kg feed agent compared with negative control group
The prevention Penaeus Vannmei of amount in vain just do not fall ill in the test group of the additive for microbe feedstuff of syndrome by Penaeus Vannmei, has
Effect protective rate has reached 100%, this is significantly better than the compound formulation of the treatment Penaeus Vannmei white excrement disease of 5g/Kg feed dosage
Effect.According to Ministry of Agriculture's publication《Fishing clinical drug test of pesticide effectiveness technical specification》In commented about the effects of antimicrobial agents
Price card is accurate：Effective percentage is effective 85% or more, and 60%-85% is effective, therefore prevents the micro- of Penaeus Vannmei just syndrome in vain
Biological feed additive prevents Penaeus Vannmei, and just the effective dose of syndrome is ultimately determined to 2.0g/Kg feeds and 3.0g/ in vain
Kg feeds.
Table 1：Prevent Penaeus Vannmei in vain just the additive for microbe feedstuff of syndrome to Penaeus Vannmei just syndrome in vain
Preventive effect
3. conclusion
The present invention is prepared for a kind of prevention Penaeus Vannmei, and just the additive for microbe feedstuff of syndrome, the microorganism are raised in vain
Feed additives safe without toxic side effect feeds 18d continuous, and feeding volume is 2%, 2.0g/Kg feeds, the 3.0g/ of shrimp total weight
To Penaeus Vannmei, just syndrome has good preventive effect in vain under Kg feed dosage, is significantly better than Enrofloxacin and treatment south
The preventive effect of the compound formulation of penaeus vannamei white excrement disease.Prevention Penaeus Vannmei prepared by the present invention just micro- life of syndrome in vain
Object feed addictive is used for the Penaeus Vannmei just prevention of syndrome in vain, and safe without toxic side effect is white with the South America of existing report
Prawn in vain just syndrome medicine (the prevention science of Penaeus Vannmei jejunum in vain just is breeded fish, 2013,2：The South America 62-62.
White shrimp is drawn and just treats an example present age aquatic products in vain, and 2011,10：It 38-38.) compares, cause of disease will not be caused as chemicals
Bacterium generates drug resistance and medicament residue, water quality and aquatic products pollution, and reported control Penaeus Vannmei can be overcome white
Just syndrome, which needs the drug more than 5 kinds such as photosynthetic bacteria+calcium oxide+Sanhuang San+Ofloxacin+sodium bicarbonate to be used cooperatively, to rise
To the problem of effect, with the Penaeus Vannmei of existing report just medicine (highly concentrated bacteriophagic Bdellovibrio original powder+height of syndrome in vain
Dense composite bacillus original powder+vitamin C original powder) ((compound formulation for the treatment of Penaeus Vannmei white excrement disease and preparation method thereof is specially
Sharp application number：201310638319.2) it) compares, operation is easier, and dosage is lower, and use cost is lower, and it is white to be more advantageous to South America
Prawn healthy aquaculture develops to be increased with cultured output.
But those of ordinary skill in the art it should be appreciated that more than, as long as the present invention connotation
In range, variation, the modification of embodiment described above will all be fallen within the scope of the present invention.
Claims (4)
1. a kind of prevention Penaeus Vannmei just additive for microbe feedstuff of syndrome in vain, it is characterised in that：By following mass ratio
Material composition：4-6 parts of bacteriophagic Bdellovibrio microcapsules, 4-6 parts of antibacterial bacillus amyloliquefaciens microcapsules；The bacteriophagic Bdellovibrio
Effective viable bacteria concentration of microcapsules is 3.69 × 107Pfu/g~3.70 × 107Pfu/g, the antibacterial bacillus amyloliquefaciens are micro-
A concentration of the 1.91 × 10 of capsule9cfu/g。
2. just the additive for microbe feedstuff of syndrome, feature exist prevention Penaeus Vannmei according to claim 1 in vain
In：5 parts of the bacteriophagic Bdellovibrio microcapsules, 5 parts of the antibacterial bacillus amyloliquefaciens microcapsules.
3. a kind of prevention Penaeus Vannmei just preparation method of the additive for microbe feedstuff of syndrome in vain, it is characterised in that：
Mass ratio according to claim 1 weighs bacteriophagic Bdellovibrio microcapsules and antibacterial bacillus amyloliquefaciens microcapsules；It will first bite
Bacterium Bdellovibrio microcapsules and antibacterial bacillus amyloliquefaciens microcapsules uniform stirring under the conditions of 15-25 DEG C mix, mixing speed
100-150r/min, mixing time 0.5-1.0h, the process of being stirred are in sealed shape,.
4. the prevention Penaeus Vannmei according to claim 3 just preparation side of the additive for microbe feedstuff of syndrome in vain
Method, it is characterised in that：5 parts of the bacteriophagic Bdellovibrio microcapsules, 5 parts of the antibacterial bacillus amyloliquefaciens microcapsules.
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Cited By (2)
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| CN111387369A (en) * | 2020-03-26 | 2020-07-10 | 江苏农林职业技术学院 | Eriocheir sinensis feed additive and method for improving fullness of Eriocheir sinensis |
| CN112375708A (en) * | 2020-11-16 | 2021-02-19 | 上海海洋大学 | Preparation for resisting white spot syndrome virus and decapod iridovirus 1 and preparation method thereof |
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| CN112375708A (en) * | 2020-11-16 | 2021-02-19 | 上海海洋大学 | Preparation for resisting white spot syndrome virus and decapod iridovirus 1 and preparation method thereof |
| CN112375708B (en) * | 2020-11-16 | 2023-05-26 | 上海海洋大学 | Preparation for resisting white spot syndrome virus and Octopus iridovirus 1 and preparation method thereof |
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