CN109329136A - It is a kind of for preventing and treating the probiotics fermention mixture and its Ecological preventive way of the rotten body disease of pond culture hybridized snakehead fish - Google Patents

It is a kind of for preventing and treating the probiotics fermention mixture and its Ecological preventive way of the rotten body disease of pond culture hybridized snakehead fish Download PDF

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Publication number
CN109329136A
CN109329136A CN201811125093.5A CN201811125093A CN109329136A CN 109329136 A CN109329136 A CN 109329136A CN 201811125093 A CN201811125093 A CN 201811125093A CN 109329136 A CN109329136 A CN 109329136A
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China
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parts
mixture
pond
probiotics fermention
water
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CN201811125093.5A
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Chinese (zh)
Inventor
赵建
陈昆慈
罗青
刘海洋
欧密
刘志军
梁剑
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Medicines In Aquaculture Trial (demonstration) Plant Of Zhujiang River Aquatic Products Research Institute
Pearl River Fisheries Research Institute CAFS
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Medicines In Aquaculture Trial (demonstration) Plant Of Zhujiang River Aquatic Products Research Institute
Pearl River Fisheries Research Institute CAFS
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Priority to CN201811125093.5A priority Critical patent/CN109329136A/en
Publication of CN109329136A publication Critical patent/CN109329136A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/13Prevention or treatment of fish diseases
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • A61K35/741Probiotics
    • A61K35/744Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10Antimycotics
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Abstract

The present invention relates to a kind of for preventing and treating the probiotics fermention mixture and its Ecological preventive way of the rotten body disease of pond culture hybridized snakehead fish, belongs to technical field of aquaculture.Probiotics fermention mixture of the invention is by 5~30 parts of viable bacteria mixed liquor, 100~200 parts of wheat bran slag, 200~300 parts of sterile water, 1~10 part of glucose composition, wherein: the viable bacteria mixed liquor is by 1~10 part of bacillus subtilis, 1~10 part of Bacillus cercus, 1~10 part of bacillus licheniformis, 1~6 part of saccharomycete, 0.5~5 part of lactic acid bacteria, 100~1000 parts of sterile water compositions.Probiotics fermention mixture of the invention is periodically applied in hybridized snakehead fish cultivating pool, and water quality adjustment is carried out by the method for the present invention, mixes and feeds lactic acid bacteria, pond water quality can be kept pure and fresh, increase dissolved oxygen in water, disease fish is gradually recovered to ingest, position of festering no longer expands and gradually heals, and finally grows skin and scale again.

Description

A kind of probiotics fermention mixture for preventing and treating the rotten body disease of pond culture hybridized snakehead fish and Its Ecological preventive way
Technical field
The invention belongs to technical field of aquaculture, are related to a kind of ecological cultivation method of hybridized snakehead fish, more specifically, this Invention is related to a kind of for preventing and treating the probiotics fermention mixture and its Ecological preventive way of the rotten body disease of pond culture hybridized snakehead fish.
Background technique
Murrel is the important fresh water Quality and economy fish in China, has the characteristics that spur is few, meat is fresh and tender, full of nutrition, has The stasis of blood is raw new, and nourishing is raised and other effects, is loved by consumers, easy to process, is one of important aquatic products in China, national annual output More than 510,000 tons.
Murrel section of China fish mainly have snakehead, snakehead and moon murrel, but cultivate mainly with the cenospecies of snakehead, snakehead and snakehead Based on, wherein based on southern the Changjiang river areas to the south cultivates with hybridized snakehead fish.Since there is murrel section fish device on the gill can directly breathe Air can carry out very high-density breeding so dissolved oxygen in water is not the factor I for limiting its cultivation amount.But high-density breeding Residual bait and excrement in water is caused largely to generate, pond waters self-purification capacity is easily deteriorated far from satisfaction, water quality, and disease is caused to send out It is raw.After disease occurs, fisherman is multi-purpose largely to change water, and frequently application disinfectant, antibiotic etc are treated, to natural water area Environment causes seriously to pollute, and causes severe drug residue problem.
The producing cause of rotten body disease is the residual bait excrement that generates in breeding process by harmful bacteria in hybridized snakehead fish breeding process It decomposes and generates ammonia nitrogen, the harmful substances such as nitrite nitrogen, to water degradation and generate toxic action to fish, reduce fish body immunity. When fish immunity degradation, adheres to the Mycophytas such as the silk capsule mould in body surface or water body intrusion fish body and quick flourish, cause Fish body table festers, and causes rotten body disease, causes mortality.In addition, fish body infection Nocard's bacillus and Aeromonas schubertii are serious When rotten body can also occur, and be to cause dead fish by gradually festering outward in vivo.
Have in the prior art using oxygenation, be directly administered and feed or add in feed for nursing compound Chinese medicinal additive prevention and treatment The method of the sick rotten body of fish, such as directly application is by Florfenicol, Amoxicillin, allicin, the mixing spice of Sanhuang San composition; Or use compound Chinese medicinal additive, such as Asiatic plantain, dandelion, Chinese gall, fructus lycii, eucommia ulmoides extracts etc., but these methods pair The effect for preventing the rotten body of sick fish is still undesirable, and the Chinese and Western medicine raw material used is not only toxic and price is high, increase cultivation at This, it is in addition long-term largely these drugs to be made to accumulate in raw fish body using drugs such as Florfenicol, Amoxicillins, it can not only make At raw fish body matter decline, other diseases are infected, the health of eater can be also endangered.Therefore, need to develop a kind of safety, The ecological agent and its control method of the environmental-friendly and efficient sick rotten body of fish of prevention and treatment.
The above-mentioned reason in basis, spy propose the application.
Summary of the invention
In view of the problems of the existing technology, the purpose of the present invention is to provide one kind for preventing and treating pond culture hybridized snakehead fish The probiotics fermention mixture and its Ecological preventive way of rotten body disease.Rotten body disease occurs in hybridized snakehead fish using method of the invention It uses in pond, is adjusted by 15~25 days water quality and intestinal health, sick fish gradually can improve and fully recover.
In order to realize above-mentioned first purpose of the invention, the present invention provides one kind for preventing and treating pond culture hybridized snakehead fish The probiotics fermention mixture of rotten body disease, the probiotics fermention mixture is by each raw material of following parts by weight through normal temperature fermentation system It is standby to form:
5~30 parts of viable bacteria mixed liquor, 100~200 parts of wheat bran slag, 200~300 parts of sterile water, 1~10 part of glucose.
Further, probiotics fermention mixture described in above-mentioned technical proposal is preferably passed through by each raw material of following parts by weight Fermentation is prepared: 10 parts of viable bacteria mixed liquor, 150 parts of wheat bran slag, 250 parts of sterile water, 5 parts of glucose.
Further, viable bacteria mixed liquor described in above-mentioned technical proposal is made of each raw material of following parts by weight:
1~10 part of bacillus subtilis, 1~10 part of Bacillus cercus, 1~10 part of bacillus licheniformis, saccharomycete 1~ 6 parts, 0.5~5 part of lactic acid bacteria, 100~1000 parts of sterile water.
Further, viable bacteria mixed liquor described in above-mentioned technical proposal is preferably made of each raw material of following parts by weight:
5~6 parts of bacillus subtilis, 5~6 parts of Bacillus cercus, 5~6 parts of bacillus licheniformis, saccharomycete 3~4 Part, 1~2 part of lactic acid bacteria, 500 parts of sterile water.
Preferably, probiotics fermention mixture as described in the above technical scheme is prepared with the following method, including Following steps:
(1) viable bacteria mixed liquor is prepared:
By bacillus subtilis, Bacillus cercus, bacillus licheniformis, saccharomycete, lactic acid bacteria and sterile water by described Parts by weight are uniformly mixed, and the viable bacteria mixed liquor is made;
(2) it ferments:
Viable bacteria mixed liquor, wheat bran slag, sterile water, glucose are mixed by the parts by weight, sealed after mixing evenly, 22 The probiotics fermention mixture is made after 24~72h of fermentation under the conditions of~38 DEG C.
Another object of the present invention is to provide probiotics fermention mixtures described above to support for bionomic control pond The method of the rotten body disease of hybridized snakehead fish is grown, described method includes following steps:
(1) pond water quality basicity and hardness are adjusted, makes water quality total alkalinity and total hardness in 130mg/L or more, and adjust Pond water calcium and magnesium hardness ratio is 3.0~3.5:1;
(2) administration of probiotics fermenting mixture: by probiotics fermention mixture by the amount of application of 1~5kg per acre splash to In pond, while compounding application photosynthetic bacteria, the amount of application of the photosynthetic bacteria are 0.5~2kg per acre;During morbidity, every 2~ It spilled once within 3 days, select apply in the morning of fine sunshine abundance, open aerator after splashing immediately until second the sky At the noon, during normal cultivation, application in every 5~10 days is primary;
(4) hybridized snakehead fish intestinal flora is adjusted:
Carp compound feed surface, 1kg feed addition 2~3g lactic acid are uniformly sprayed at into after lactic acid bacteria is dissolved with sterile water Bacterium, feed spray lactic acid bacteria and after mixing evenly, feed after drying in the cool, adhere to feeding daily during morbidity and mixed lactic acid The feed of bacterium 1~3 time is mixed and is fed 1 time during normal cultivation for 2~3 days.
Further, above-mentioned technical proposal is adjusting pond water if rotten body disease has been broken out in the pond of cultivation hybridized snakehead fish It before matter basicity and hardness, also needs to carry out change water to process to pond, former pool water is discharged 1/3 or more, and it is fresh dry to fill equivalent Water purification, changing water helps quickly to reduce poisonous and harmful substance concentration in water, slows down disease expansion, and accelerate disease fish resume speed; If it is normal cultivation, then without changing water operation.
Further, the calcium and magnesium hardness ratio of pond water quality is by splashing into pond in above-mentioned technical proposal step (2) Quick lime, dolomite dust are adjusted.
Compared with prior art, a kind of probiotics for preventing and treating the rotten body disease of pond culture hybridized snakehead fish of the present invention sends out Ferment mixture and its Ecological preventive way have the following beneficial effects:
Probiotics fermention mixture of the invention is periodically used in normal hybridisation murrel cultivating pool, pond water can be kept Matter is pure and fresh, and probiotics fermention mixture of the invention has certain fertilizer degree, and beneficial microbe mass propagation is in the ascendance, pond In time by beneficial bacterium and decomposition, nitrite converts in time for pool residual bait and excrement, is maintained at low concentration, algae absorbs decomposition Nutriment afterwards, mass propagation carry out photosynthesis and discharge a large amount of oxygen, increase pond dissolved oxygen in water.Algae in pond Mass propagation inhibits the generation of harmful bacteria, helminth.Pond dissolved oxygen is sufficient simultaneously, and healthy fish situation is preferable, resistance By force.In the pathogenetic pond of rotten body, carries out water quality adjustment according to the method for the present invention and mix to feed lactic acid bacteria, pond water quality guarantee can be made Hold it is pure and fresh, increase dissolved oxygen in water, be gradually recovered disease fish and ingest, position of festering no longer expands and gradually heals, finally again Grow skin and scale.
Detailed description of the invention
Fig. 1 is wound photo when the rotten body disease fish of 1 hybridized snakehead fish of Application Example of the present invention falls ill;
Fig. 2 (a), (b) are respectively the wound photo after the rotten body disease fish disease hair recovery from illness of 1 hybridized snakehead fish of Application Example of the present invention.
Specific embodiment
Below with reference to case study on implementation and attached drawing, invention is further described in detail.The implementation case is with skill of the present invention Implemented under premised on art, provides detailed embodiment and specific operating process now to illustrate that the present invention has and create Property, but protection scope of the present invention case study on implementation not limited to the following.
The information for including according to the application, to those skilled in the art can be easily to essence of the invention Really description carries out various changes, without departing from spirit and scope of the appended claims.It should be understood that the scope of the present invention is not Process, property defined by being confined to or component, because these embodiments and other descriptions are just for the sake of schematic Illustrate certain aspects of the present disclosure.In fact, this field or those skilled in the relevant art obviously can be to embodiment party of the present invention The various changes that formula is made all cover within the scope of the appended claims.
It is not intended to limit the scope of the invention for a better understanding of the present invention, expression dosage used in this application, All numbers of percentage and other numerical value, are understood to be modified with word " about " in all cases.Therefore, Unless stated otherwise, otherwise digital parameters listed in specification and appended book are all approximations, may It can be changed according to the difference for the desirable properties for attempting to obtain.Each digital parameters at least should be considered as according to being reported Effective digital and obtained by the conventional method of rounding up.
Embodiment 1
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is described prebiotic Bacterium fermenting mixture is prepared by each raw material of following parts by weight through normal temperature fermentation:
10 parts of viable bacteria mixed liquor, 150 parts of wheat bran slag, 250 parts of sterile water, 5 parts of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
5 parts of bacillus subtilis, 5 parts of Bacillus cercus, 5 parts of bacillus licheniformis, 3 parts of saccharomycete, 1 part of lactic acid bacteria, 500 parts of sterile water.
Probiotics fermention mixture described above is specifically prepared with the following method, includes the following steps:
(1) viable bacteria mixed liquor is prepared:
By bacillus subtilis, Bacillus cercus, bacillus licheniformis, saccharomycete, lactic acid bacteria and sterile water by described Parts by weight are uniformly mixed, and the viable bacteria mixed liquor is made;
(2) it ferments:
Viable bacteria mixed liquor, wheat bran slag, sterile water, glucose are mixed by the parts by weight, sealed after mixing evenly, 35 The probiotics fermention mixture is made after fermentation 48h under the conditions of DEG C.
Embodiment 2
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is described prebiotic Bacterium fermenting mixture is prepared by each raw material of following parts by weight through normal temperature fermentation:
10 parts of viable bacteria mixed liquor, 150 parts of wheat bran slag, 250 parts of sterile water, 5 parts of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
6 parts of bacillus subtilis, 6 parts of Bacillus cercus, 6 parts of bacillus licheniformis, 4 parts of saccharomycete, 2 parts of lactic acid bacteria, 500 parts of sterile water.
Probiotics fermention mixture described above is specifically prepared with the following method, includes the following steps:
(1) viable bacteria mixed liquor is prepared:
By bacillus subtilis, Bacillus cercus, bacillus licheniformis, saccharomycete, lactic acid bacteria and sterile water by described Parts by weight are uniformly mixed, and the viable bacteria mixed liquor is made;
(2) it ferments:
Viable bacteria mixed liquor, wheat bran slag, sterile water, glucose are mixed by the parts by weight, sealed after mixing evenly, 30 The probiotics fermention mixture is made after fermentation 36h under the conditions of DEG C.
Embodiment 3
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is described prebiotic Bacterium fermenting mixture is prepared by each raw material of following parts by weight through normal temperature fermentation:
5 parts of viable bacteria mixed liquor, 100 parts of wheat bran slag, 200 parts of sterile water, 1 part of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
1 part of bacillus subtilis, 1 part of Bacillus cercus, 1 part of bacillus licheniformis, 1 part of saccharomycete, lactic acid bacteria 0.5 Part, 100 parts of sterile water.
Probiotics fermention mixture described above is specifically prepared with the following method, includes the following steps:
(1) viable bacteria mixed liquor is prepared:
By bacillus subtilis, Bacillus cercus, bacillus licheniformis, saccharomycete, lactic acid bacteria and sterile water by described Parts by weight are uniformly mixed, and the viable bacteria mixed liquor is made;
(2) it ferments:
Viable bacteria mixed liquor, wheat bran slag, sterile water, glucose are mixed by the parts by weight, sealed after mixing evenly, 22 The probiotics fermention mixture is made after fermentation 72h under the conditions of DEG C.
Embodiment 4
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is described prebiotic Bacterium fermenting mixture is prepared by each raw material of following parts by weight through normal temperature fermentation:
30 parts of viable bacteria mixed liquor, 200 parts of wheat bran slag, 300 parts of sterile water, 10 parts of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
10 parts of bacillus subtilis, 10 parts of Bacillus cercus, 10 parts of bacillus licheniformis, 6 parts of saccharomycete, lactic acid bacteria 5 Part, 1000 parts of sterile water.
Probiotics fermention mixture described above is specifically prepared with the following method, includes the following steps:
(1) viable bacteria mixed liquor is prepared:
By bacillus subtilis, Bacillus cercus, bacillus licheniformis, saccharomycete, lactic acid bacteria and sterile water by described Parts by weight are uniformly mixed, and the viable bacteria mixed liquor is made;
(2) it ferments:
Viable bacteria mixed liquor, wheat bran slag, sterile water, glucose are mixed by the parts by weight, sealed after mixing evenly, 38 It ferments under the conditions of DEG C and the probiotics fermention mixture is made afterwards for 24 hours.
Embodiment 5
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, the mixing The preparation method of object is same as Example 1, and difference is only that raw material dosage difference, each raw material dosage of the present embodiment specifically:
15 parts of viable bacteria mixed liquor, 120 parts of wheat bran slag, 220 parts of sterile water, 2 parts of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
2 parts of bacillus subtilis, 4 parts of Bacillus cercus, 3 parts of bacillus licheniformis, 2 parts of saccharomycete, 1 part of lactic acid bacteria, 200 parts of sterile water.
Embodiment 6
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, the mixing The preparation method of object is same as Example 1, and difference is only that raw material dosage difference, each raw material dosage of the present embodiment specifically:
20 parts of viable bacteria mixed liquor, 140 parts of wheat bran slag, 240 parts of sterile water, 4 parts of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
3 parts of bacillus subtilis, 3 parts of Bacillus cercus, 4 parts of bacillus licheniformis, 3 parts of saccharomycete, 2 parts of lactic acid bacteria, 400 parts of sterile water.
Embodiment 7
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, the mixing The preparation method of object is same as Example 1, and difference is only that raw material dosage difference, each raw material dosage of the present embodiment specifically:
25 parts of viable bacteria mixed liquor, 160 parts of wheat bran slag, 260 parts of sterile water, 6 parts of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
4 parts of bacillus subtilis, 6 parts of Bacillus cercus, 7 parts of bacillus licheniformis, 5 parts of saccharomycete, 3 parts of lactic acid bacteria, 600 parts of sterile water.
Embodiment 8
The present embodiment it is a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, the mixing The preparation method of object is same as Example 1, and difference is only that raw material dosage difference, each raw material dosage of the present embodiment specifically:
28 parts of viable bacteria mixed liquor, 180 parts of wheat bran slag, 280 parts of sterile water, 8 parts of glucose;
Wherein: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
6 parts of bacillus subtilis, 8 parts of Bacillus cercus, 9 parts of bacillus licheniformis, 4 parts of saccharomycete, 4 parts of lactic acid bacteria, 800 parts of sterile water.
Application Example 1
Shuande agricultural science and technology Co., Ltd cultivates 200 mu of hybridized snakehead fish.Wherein two mouthfuls of pools, 4 mu and 8 mu respectively, in 2016 On July 3, in puts seedling cultivation, puts 40,000 and 80,000 tails respectively.On April 10th, 2017, when cultivation fish counterpoise reaches 700g or so, outburst All there is squama and fester in rotten body disease, sick fish body table and head, and white water mold is grown on the surface of a wound.Sick fish slow movement, ingests It reduces or stops, beginning dead fish on the 15th, referring to attached drawing 1.From April 20, water quality adjustment is carried out to pond, and embodiment 1 is made The probiotics fermention mixture obtained is used for the ecosystem cure of the rotten body disease of pond culture hybridized snakehead fish,
Specific treatment method includes the following steps:
(1) water is changed
Former fishpond water is discharged 1/3 or more, and fills equivalent fresh clean water.Change water help quickly to reduce it is toxic in water Concentration of narmful substance slows down disease expansion, and accelerates disease fish resume speed.
(2) water body basicity and hardness are adjusted
Before carrying out water quality adjustment, total alkalinity and total hardness first in measurement cultivating pool.Using titration, can also be used total Basicity, Hardness Determination instrument are measured.If total alkalinity and total hardness are in 130mg/L or more, without adjusting.If lower than with Upper minimum numerical value then first has to adjust total alkalinity and total hardness.Total alkalinity and total hardness, which are adjusted, in pond water generally uses quick lime With dolomite dust full pool spilling head, 1 meter of depth quick lime 5kg used for ponds, dolomite dust 10kg, increase total hardness and total alkali per acre Degree, while adjusting calcium and magnesium hardness ratio is 3.0~3.5:1.
(3) administration of probiotics tunning
It is used in the pond of cultivation hybridized snakehead fish, probiotics fermention product is splashed 3kg per acre, and photosynthetic bacteria is administered simultaneously 1kg is used cooperatively, and spilled once within every 2~3 days, selects apply in the morning of fine sunshine abundance, is opened and is increased immediately after splashing Oxygen machine is until the next morning.
(4) lactic acid bacteria adjusts intestinal flora
Mixed feed surface, every 1kg feed addition 3g lactic acid bacteria, feed are uniformly sprayed at after dissolving lactic acid bacteria with sterile water Lactic acid bacteria and after mixing evenly is sprayed, is fed after drying in the cool, adheres to feeding daily feed 2 times that mixed lactic acid bacteria, He is identical as standard hybridization murrel cultivating condition.
To May 2, no longer there is dead fish, until sick fish on the 5th gradually starts to ingest.To May 20, feedstuff feeding amount was gradually Before the onset of being restored to, the sick rotten body surface of a wound water mold reduction of fish starts to occur red and heal, referring to attached drawing 2 (a), (b) photo.To 10 The moon 9, cultivation fish average weight reach 1kg or more, and vending articles fish 42142.5kg and 90425kg are distinguished on two mouthfuls of pools.
Application Example 2
In May, 2016~2017 year December, in the village Shunde Le Liumou, totally 500 mu of hybridized snakehead fish cultivating pool, surrounding village About cultivating pool is up to 1200 mu or so.Wherein 200 mu of ponds are sent out using probiotics obtained made from the embodiment of the present invention 2~8 Ferment mixture is normally cultivated, and specific cultural method includes the following steps:
(1) water body basicity and hardness are adjusted
Before carrying out water quality adjustment, total alkalinity and total hardness first in measurement cultivating pool.Using titration, can also be used total Basicity, Hardness Determination instrument are measured.If total alkalinity and total hardness are in 130mg/L or more, without adjusting.If lower than with Upper minimum numerical value then first has to adjust total alkalinity and total hardness.Total alkalinity and total hardness, which are adjusted, in pond water generally uses quick lime With dolomite dust full pool spilling head, 1 meter of depth 5kg used for ponds, while compounding application dolomite dust 10kg per acre adjusts calcium and magnesium hardness Than for 3.0~3.5:1.
(2) administration of probiotics tunning
It is used in the pond of cultivation hybridized snakehead fish, probiotics fermention product is splashed 1~5kg per acre, is administered simultaneously photosynthetic thin 0.5~2kg of bacterium, is used cooperatively, and application in every 5~10 days is primary, selects apply in the morning of fine sunshine abundance, stands after splashing Aerator is opened until the next morning.
(3) lactic acid bacteria adjusts intestinal flora
Mixed feed surface is uniformly sprayed at after dissolving lactic acid bacteria with sterile water, every 1kg feed addition 2~3g lactic acid bacteria, Feed sprays lactic acid bacteria and after mixing evenly, feeds after drying in the cool, the feed 1 for mixing lactic acid bacteria was fed every 2~3 days Secondary, other are identical as standard hybridization murrel cultivating condition.
The hybridized snakehead fish cultivated using Ecological preventive way of the invention, whole process do not break out disease, and cultivation survival rate is up to 90%. And serious rotten body disease has been broken out in the pond that surrounding commonsense method is cultivated in March, 2017, the death rate is caused up to 30% or more Serious financial consequences.

Claims (8)

1. a kind of for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is characterised in that: described prebiotic Bacterium fermenting mixture is prepared by each raw material of following parts by weight through normal temperature fermentation:
5~30 parts of viable bacteria mixed liquor, 100~200 parts of wheat bran slag, 200~300 parts of sterile water, 1~10 part of glucose.
2. it is according to claim 1 for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is special Sign is: the probiotics fermention mixture fermented is prepared by each raw material of following parts by weight: 10 parts of viable bacteria mixed liquor, 150 parts of wheat bran slag, 250 parts of sterile water, 5 parts of glucose.
3. it is according to claim 1 for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is special Sign is: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
1~10 part of bacillus subtilis, 1~10 part of Bacillus cercus, 1~10 part of bacillus licheniformis, 1~6 part of saccharomycete, 0.5~5 part of lactic acid bacteria, 100~1000 parts of sterile water.
4. it is according to claim 3 for preventing and treating the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish, it is special Sign is: the viable bacteria mixed liquor is made of each raw material of following parts by weight:
5~6 parts of bacillus subtilis, 5~6 parts of Bacillus cercus, 5~6 parts of bacillus licheniformis, 3~4 parts of saccharomycete, cream 1~2 part of sour bacterium, 500 parts of sterile water.
5. as claimed in claim 4 for preventing and treating the preparation side of the probiotics fermention mixture of the rotten body disease of pond culture hybridized snakehead fish Method, it is characterised in that: described method includes following steps:
(1) viable bacteria mixed liquor is prepared:
Bacillus subtilis, Bacillus cercus, bacillus licheniformis, saccharomycete, lactic acid bacteria and sterile water are pressed into the weight Part is uniformly mixed, and the viable bacteria mixed liquor is made;
(2) it ferments:
Viable bacteria mixed liquor, wheat bran slag, sterile water, glucose are mixed by the parts by weight, sealed after mixing evenly, 22~38 The probiotics fermention mixture is made after 24~72h of fermentation under the conditions of DEG C.
6. the described in any item probiotics fermention mixtures of Claims 1 to 4 are used for the rotten body of bionomic control pond culture hybridized snakehead fish The method of disease, it is characterised in that: described method includes following steps:
(1) pond water quality basicity and hardness are adjusted, makes water quality total alkalinity and total hardness in 130mg/L or more, and adjust pond Water calcium and magnesium hardness ratio is 3.0~3.5;
(2) administration of probiotics fermenting mixture: probiotics fermention mixture is splashed by the amount of application of 1~5kg per acre to pond In, while compounding application photosynthetic bacteria, the amount of application of the photosynthetic bacteria are 0.5~2kg per acre;During morbidity, every 2~3 days It spilled once, select apply in the morning of fine sunshine abundance, open aerator after splashing immediately until the next morning, just Often during cultivation, application in every 5~10 days is primary;
(3) hybridized snakehead fish intestinal flora is adjusted:
Uniformly it is sprayed at into carp compound feed surface after lactic acid bacteria is dissolved with sterile water, 1kg feed addition 2~3g lactic acid bacteria, Feed sprays lactic acid bacteria and after mixing evenly, feeds after drying in the cool, adheres to feeding daily during morbidity and mixed lactic acid bacteria Feed 1~3 time, during normal cultivation, mix within 2~3 days and feed 1 time.
7. the side that probiotics fermention mixture according to claim 6 is used for the rotten body disease of bionomic control pond culture hybridized snakehead fish Method, it is characterised in that: if rotten body disease has been broken out in the pond of cultivation hybridized snakehead fish, adjust pond water quality basicity and hardness it Before, it also needs to carry out change water to process to pond, former pool water is discharged 1/3 or more, and fill equivalent fresh clean water;Normal cultivation nothing Water step need to be changed.
8. the side that probiotics fermention mixture according to claim 6 is used for the rotten body disease of bionomic control pond culture hybridized snakehead fish Method, it is characterised in that: the calcium and magnesium hardness ratio of pond water quality is by quick lime of splashing into pond, dolomite dust in step (2) It is adjusted.
CN201811125093.5A 2018-09-26 2018-09-26 It is a kind of for preventing and treating the probiotics fermention mixture and its Ecological preventive way of the rotten body disease of pond culture hybridized snakehead fish Pending CN109329136A (en)

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CN112753891A (en) * 2021-01-19 2021-05-07 广州飞禧特生物科技有限公司 Novel fermented feed for hybrid raw fish fry and preparation method thereof

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CN107736281A (en) * 2017-09-29 2018-02-27 中国水产科学研究院珠江水产研究所 A kind of method that pond culture mandarin fish parasite is prevented and treated using probiotics fermention soy sauce residues
CN108040955A (en) * 2018-02-11 2018-05-18 水利部中国科学院水工程生态研究所 A kind of simple circulating water cultivation method and device of C. guichenoti seedling

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Publication number Priority date Publication date Assignee Title
KR20100123972A (en) * 2009-05-18 2010-11-26 양영선 Anion porous artifical
CN106912412A (en) * 2017-05-15 2017-07-04 上海彰显渔业专业合作社 A kind of seven color angle fish indoor intensive recirculated water high-efficiency breeding method
CN107736281A (en) * 2017-09-29 2018-02-27 中国水产科学研究院珠江水产研究所 A kind of method that pond culture mandarin fish parasite is prevented and treated using probiotics fermention soy sauce residues
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112753891A (en) * 2021-01-19 2021-05-07 广州飞禧特生物科技有限公司 Novel fermented feed for hybrid raw fish fry and preparation method thereof

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