CN110881429A - Mixed culture method for tilapia mossambica and penaeus vannamei boone - Google Patents

Mixed culture method for tilapia mossambica and penaeus vannamei boone Download PDF

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CN110881429A
CN110881429A CN201911130879.0A CN201911130879A CN110881429A CN 110881429 A CN110881429 A CN 110881429A CN 201911130879 A CN201911130879 A CN 201911130879A CN 110881429 A CN110881429 A CN 110881429A
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water
parts
fish
tilapia
feed
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李芳远
佟延南
朱海
赵光军
申志新
董杨
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HAINAN ACADEMY OF OCEAN AND FISHERIES SCIENCES
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A23K10/00Animal feeding-stuffs
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
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    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/50Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
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    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F2303/00Specific treatment goals
    • C02F2303/04Disinfection
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    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract

The invention discloses a method for polyculture of tilapia and penaeus vannamei boone, which comprises the following steps of S1, preparation work before stocking: cleaning the sewage, sterilizing, removing harm, killing harmful organisms in the pond, installing a water inlet and drainage filter screen and a purse net with thick marks, and draining water after the installation is finished to sterilize a water body; s2, water cultivation: after 3 days of water body disinfection, fertilizer is uniformly sprayed on the water surface after being dissolved by water, topdressing can be continuously carried out for 1 time every day until the transparency of pool water is about 25cm, and after water fertilization, a probiotic preparation is applied 2 days before seedling placement to keep the balance of the water body micro-ecological environment; before mixed culture of tilapia and penaeus vannamei boone, the tilapia is subjected to primary disinfection and sterilization, germs carried by the tilapia are reduced, diseases are reduced, and the survival rate of the fish and the shrimp is improved.

Description

Mixed culture method for tilapia mossambica and penaeus vannamei boone
Technical Field
The invention relates to the technical field of cultivation, in particular to a method for polyculture of tilapia and penaeus vannamei boone.
Background
The tilapia is commonly named as Nanyang crucian carp, African crucian carp, non-crucian carp, Vietnam fish, Nanyang crucian carp and the like. The primordial line refers to the non-crucian fish species hatched in the Mosangbick mode production area. Generally, the water-saving agent can live in fresh water, can also live in saline water with different salt contents, and can also live in shallow water of lakes, rivers and ponds. It has strong adaptability, can be propagated in water area with small area, can be grown even in paddy field, and has strong adaptability to water with less dissolved oxygen. Most tilapia mossambica are omnivorous and often eat plants and debris in water. In southern coastal areas, abundant brackish water resources are provided, and the salinity difference of water bodies is large due to the influence of seasons, so that the attempts of polyculturing tilapia besides silver carp, bighead carp, crucian carp and other conventional fishes are made while the penaeus vannamei boone is bred, and a mature breeding mode is formed.
The existing method for mixed culture of tilapia and penaeus vannamei boone has the advantages that the condition that the tilapia is sick easily occurs in the actual culture process, the survival rate of the tilapia and the penaeus vannamei boone is reduced, the yield is reduced, when the tilapia is put in, the tilapia carries pathogens, the pathogens have small influence on the tilapia, but have large influence on the seedlings of the penaeus vannamei boone, and the penaeus vannamei boone is easy to be sick.
Disclosure of Invention
The invention aims to provide a method for mixed culture of tilapia and penaeus vannamei, which aims to solve the problems that the existing method for mixed culture of tilapia and penaeus vannamei in the background art is easy to cause the disease of the tilapia and the penaeus vannamei in the actual culture process, reduces the survival rate of the tilapia and the yield, wherein when the tilapia is put in, the tilapia carries pathogens, the pathogens have little influence on the tilapia mossambica, but have great influence on the seedlings of the penaeus vannamei, and the penaeus vannamei is easy to cause the disease of the penaeus vannamei.
In order to achieve the purpose, the invention provides the following technical scheme: a method for polyculture of tilapia and penaeus vannamei boone comprises the following steps:
s1, preparation before seedling release: cleaning the sewage, sterilizing, removing harm, killing harmful organisms in the pond, installing a water inlet and drainage filter screen and a purse net with thick marks, and draining water after the installation is finished to sterilize a water body;
s2, water cultivation: after 3 days of water body disinfection, fertilizer is uniformly sprayed on the water surface after being dissolved by water, topdressing can be continuously carried out for 1 time every day until the transparency of pool water is about 25cm, and after water fertilization, a probiotic preparation is applied 2 days before seedling placement to keep the balance of the water body micro-ecological environment;
s3, seed putting: when the water temperature is stable above 18 ℃, putting the penaeus vannamei boone into a fish pond, then putting the tilapia, before putting the tilapia, adopting a disinfection solvent to disinfect the tilapia, putting the fish fries into water added with the disinfection solvent, soaking for 10-20 minutes, putting the fish fries 3-4 times and the shrimp fries 5-7 times into a pond water body, wherein the time interval of putting the fish fries is 5-10 days, the time interval of putting the shrimp fries is 3-6 days, starting an aerator after 3 months of putting the fries, and the aeration time is 3-5 hours;
s4, seedling culture: feeding the shrimp fries 2 days after the shrimp fries are put into the pond, feeding 30-50 g of the feed for every 10 ten thousand shrimp fries 5 days before, feeding for 4 times every day, and increasing the feeding amount by 20 percent every day; and after 2-3 days of first fry feeding, feeding fish feed to the pond water body according to the daily feeding amount of 2-7% of fish weight, and after continuously feeding for 30-40 days, feeding the fish and shrimp mixed feed to the pond water body.
Preferably, the fertilizer used in the water body cultivation is a mixture of urea, a nitrogen fertilizer, a phosphate fertilizer, a potash fertilizer and calcium carbonate, and 3 parts of urea, 3 parts of nitrogen fertilizer, 3 parts of phosphate fertilizer, 3 parts of potash fertilizer and 3 parts of calcium carbonate are taken, and the materials are put into a stirrer to be fully stirred and uniformly mixed.
Preferably, the fish and shrimp mixed feed consists of fish feed and shrimp feed, and the ratio of the fish feed to the shrimp feed is 3: 1.
Preferably, the preparation of the fish and shrimp mixed feed comprises the steps of weighing the fish feed and the shrimp feed in proportion, adding water into the weighed fish feed, stirring, adding the shrimp feed after stirring for 10 minutes, and continuing stirring for 15 minutes to obtain the fish and shrimp mixed feed.
Preferably, the fish feed consists of the following components: 7-12 parts of fish meal, 5-15 parts of wheat bran, 10-15 parts of wheat bran powder, 15-21 parts of corn protein powder, 15-21 parts of bean cake protein powder, 3-6 parts of earthworm powder, 4-6 parts of cinnamon powder, 3-5 parts of a vitamin mixture and 1.5-3.5 parts of a mineral additive.
Preferably, when the fish feed is prepared, fish meal, wheat bran powder, corn protein powder and bean cake protein powder are poured into a container to be stirred, after the fish meal, the wheat bran powder, the corn protein powder and the bean cake protein powder are uniformly mixed, the rest earthworm powder, the cassia bark powder, the vitamin mixing agent and the mineral additive are poured into the container to be stirred, after the fish feed is uniformly stirred, a small amount of deionized water is added to soften the fish feed, and the fish feed is stirred simultaneously.
Preferably, the shrimp feed consists of the following components: 20-30 parts of corn flour, 15-35 parts of wheat bran powder, 13-26 parts of zymoprotein, 10-21 parts of silkworm chrysalis, 2-4 parts of bacillus subtilis, 1-4 parts of selenium-rich yeast and 2-3 parts of compound vitamin.
Preferably, the preparation method of the shrimp feed comprises the steps of pouring corn flour, wheat bran powder, enzymolysis protein and silkworm chrysalis into a container, stirring, adding a proper amount of warm water with the temperature of 35 ℃ after stirring, continuously stirring, adding bacillus subtilis, selenium-enriched yeast and compound vitamins, and continuously and uniformly mixing.
Preferably, the disinfectant solvent adopts 20ppm potassium permanganate and 8ppm copper sulfate and proper amount of water, potassium permanganate: copper sulfate: the proportion of proper amount of water is 0.2: 0.1: 10.
the invention provides a method for polyculture of tilapia and penaeus vannamei boone, which has the following beneficial effects:
(1) according to the invention, before mixed culture of tilapia and penaeus vannamei boone, primary disinfection and sterilization are carried out on tilapia, germs carried by tilapia are reduced, diseases are reduced, and the survival rate of fish and shrimp is improved.
(2) The fish and shrimp mixed feed provided by the invention has rich protein and multiple vitamins, so that the proportion of various nutrient components is moderate, and the fish and shrimp mixed feed has good palatability, easy absorption, high growth speed and strong disease resistance, and is suitable for two varieties of tilapia and penaeus vannamei.
Detailed Description
Example 1
A method for polyculture of tilapia and penaeus vannamei boone comprises the following steps:
s1, preparation before seedling release: cleaning the sewage, sterilizing, removing harm, killing harmful organisms in the pond, installing a water inlet and drainage filter screen and a purse net with thick marks, and draining water after the installation is finished to sterilize a water body;
s2, water cultivation: after 3 days of water body disinfection, 3 parts of urea, 3 parts of nitrogenous fertilizer, 3 parts of phosphate fertilizer, 3 parts of potash fertilizer and 3 parts of calcium carbonate are uniformly mixed to prepare fertilizer, then the fertilizer is uniformly sprayed on the water surface after being dissolved by water, topdressing can be continuously carried out for 1 time every day until the transparency of pool water is about 25cm, and after water fertilization, a probiotic preparation is applied 2 days before seedling placement to keep the balance of the water body micro-ecological environment;
s3, seed putting: when the water temperature is stabilized to be more than 18 ℃, the penaeus vannamei boone is firstly put into a fish pond, then the tilapia is put into the fish pond, and before the tilapia is put into the fish pond, 20ppm of potassium permanganate, 8ppm of copper sulfate and a proper amount of water are mixed according to the weight ratio of 0.2: 0.1: 10, then placing the fry in water added with a disinfection solvent for immersion washing for 10 minutes, putting the fry and shrimp fries into a fishpond for 3 times and 5 times in the fry throwing process, wherein the time interval of putting the fry is 5 days, the time interval of putting the shrimp fries is 3 days, starting an aerator for 3 hours every day in the 3 rd month after putting the fries;
s4, seedling culture: pouring fish meal, wheat bran powder, corn protein powder and bean cake protein powder into a container for stirring, after uniform mixing, pouring the rest earthworm powder, cassia bark powder, vitamin mixing agent and mineral additive into the container for stirring, after uniform stirring, adding a small amount of deionized water for softening, and simultaneously stirring to prepare fish feed, pouring corn flour, wheat bran powder, enzymolysis protein and silkworm chrysalis into the container for stirring, adding a proper amount of warm water with the temperature of 35 ℃ for continuous stirring, then adding bacillus subtilis, selenium-enriched yeast and compound vitamin for continuous uniform mixing to prepare fish feed, weighing the fish feed and shrimp feed according to the proportion of 3:1, adding water into the weighed fish feed, stirring, adding shrimp feed after 10 minutes of stirring, continuously stirring for 15 minutes to obtain fish and shrimp mixed feed, starting to feed on the 2 nd day after shrimp fries are put into a pool, feeding 30-50 g of shrimp feed every 10 million shrimp larvae in the first 5 days, feeding 4 times every day, and increasing the feeding amount by 20% every day; and after 2-3 days of first fry feeding, feeding fish feed to the pond water body according to the daily feeding amount of 2-7% of fish weight, and after continuously feeding for 30-40 days, feeding the fish and shrimp mixed feed to the pond water body.
Example 2
A method for polyculture of tilapia and penaeus vannamei boone comprises the following steps:
s1, preparation before seedling release: cleaning the sewage, sterilizing, removing harm, killing harmful organisms in the pond, installing a water inlet and drainage filter screen and a purse net with thick marks, and draining water after the installation is finished to sterilize a water body;
s2, water cultivation: after 3 days of water body disinfection, 3 parts of urea, 3 parts of nitrogenous fertilizer, 3 parts of phosphate fertilizer, 3 parts of potash fertilizer and 3 parts of calcium carbonate are uniformly mixed to prepare fertilizer, then the fertilizer is uniformly sprayed on the water surface after being dissolved by water, topdressing can be continuously carried out for 1 time every day until the transparency of pool water is about 25cm, and after water fertilization, a probiotic preparation is applied 2 days before seedling placement to keep the balance of the water body micro-ecological environment;
s3, seed putting: when the water temperature is stabilized to be more than 18 ℃, the penaeus vannamei boone is firstly put into a fish pond, then the tilapia is put into the fish pond, and before the tilapia is put into the fish pond, 20ppm of potassium permanganate, 8ppm of copper sulfate and a proper amount of water are mixed according to the weight ratio of 0.2: 0.1: 10, then putting the fry in water added with a disinfection solvent for immersion washing for 20 minutes, putting the fry in a fishpond for 4 times and 7 times in the fry putting process, wherein the time interval of putting the fry is 10 days, the time interval of putting the fry is 6 days, and starting an aerator for 5 hours every day in the 3 rd month after putting the fry;
s4, seedling culture: s4, seedling culture: pouring fish meal, wheat bran powder, corn protein powder and bean cake protein powder into a container for stirring, after uniform mixing, pouring the rest earthworm powder, cassia bark powder, vitamin mixing agent and mineral additive into the container for stirring, after uniform stirring, adding a small amount of deionized water for softening, and simultaneously stirring to prepare fish feed, pouring corn flour, wheat bran powder, enzymolysis protein and silkworm chrysalis into the container for stirring, adding a proper amount of warm water with the temperature of 35 ℃ for continuous stirring, then adding bacillus subtilis, selenium-enriched yeast and compound vitamin for continuous uniform mixing to prepare fish feed, weighing the fish feed and shrimp feed according to the proportion of 3:1, adding water into the weighed fish feed, stirring, adding shrimp feed after 10 minutes of stirring, continuously stirring for 15 minutes to obtain fish and shrimp mixed feed, starting to feed on the 2 nd day after shrimp fries are put into a pool, feeding 30-50 g of shrimp feed every 10 million shrimp larvae in the first 5 days, feeding 4 times every day, and increasing the feeding amount by 20% every day; and after 2-3 days of first fry feeding, feeding fish feed to the pond water body according to the daily feeding amount of 2-7% of fish weight, and after continuously feeding for 30-40 days, feeding the fish and shrimp mixed feed to the pond water body.
Comparative example 1
A method for polyculture of tilapia and penaeus vannamei boone comprises the following steps:
s1, preparation before seedling release: cleaning the sewage, sterilizing, removing harm, killing harmful organisms in the pond, installing a water inlet and drainage filter screen and a purse net with thick marks, and draining water after the installation is finished to sterilize a water body;
s2, water cultivation: after 3 days of water body disinfection, 3 parts of urea, 3 parts of nitrogenous fertilizer, 3 parts of phosphate fertilizer, 3 parts of potash fertilizer and 3 parts of calcium carbonate are uniformly mixed to prepare fertilizer, then the fertilizer is uniformly sprayed on the water surface after being dissolved by water, topdressing can be continuously carried out for 1 time every day until the transparency of pool water is about 25cm, and after water fertilization, a probiotic preparation is applied 2 days before seedling placement to keep the balance of the water body micro-ecological environment;
s3, seed putting: when the water temperature is stable above 18 ℃, putting the penaeus vannamei boone into a fish pond, then putting the tilapia, putting 3-4 times of fish fries and 5-7 times of shrimp fries in the fry putting process, wherein the time interval of putting the fish fries is 5-10 days, the time interval of putting the shrimp fries is 3-6 days, starting an aerator for 3-5 hours every day in the 3 rd month after the fry putting;
s4, seedling culture: pouring fish meal, wheat bran powder, corn protein powder and bean cake protein powder into a container for stirring, after uniform mixing, pouring the rest earthworm powder, cassia bark powder, vitamin mixing agent and mineral additive into the container for stirring, after uniform stirring, adding a small amount of deionized water for softening, and simultaneously stirring to prepare fish feed, pouring corn flour, wheat bran powder, enzymolysis protein and silkworm chrysalis into the container for stirring, adding a proper amount of warm water with the temperature of 35 ℃ for continuous stirring, then adding bacillus subtilis, selenium-enriched yeast and compound vitamin for continuous uniform mixing to prepare fish feed, weighing the fish feed and shrimp feed according to the proportion of 3:1, adding water into the weighed fish feed, stirring, adding shrimp feed after 10 minutes of stirring, continuously stirring for 15 minutes to obtain fish and shrimp mixed feed, starting to feed on the 2 nd day after shrimp fries are put into a pool, feeding 30-50 g of shrimp feed every 10 million shrimp larvae in the first 5 days, feeding 4 times every day, and increasing the feeding amount by 20% every day; and after 2-3 days of first fry feeding, feeding fish feed to the pond water body according to the daily feeding amount of 2-7% of fish weight, and after continuously feeding for 30-40 days, feeding the fish and shrimp mixed feed to the pond water body.
The statistical results of the survival rate and the yield of the fishes and shrimps in the examples 1 and 2 and the comparative example 1 of the invention are shown in the table,
survival rate of fish (%) Fish yield (Kg/mu) Shrimp survival rate (%) Shrimp yield (Kg/mu)
Example 1 93.71 4121 83.23 312
Example 2 94.42 4245 85.91 324
Comparative example 1 71.33 3021 60.21 298
As can be seen from the table, the survival rate of the fish and the survival rate of the shrimps are both improved by adopting the culture method, and the yield is increased, so that the culture method has good benefit.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. A method for polyculture of tilapia and penaeus vannamei boone is characterized by comprising the following steps:
s1, preparation before seedling release: cleaning the sewage, sterilizing, removing harm, killing harmful organisms in the pond, installing a water inlet and drainage filter screen and a purse net with thick marks, and draining water after the installation is finished to sterilize a water body;
s2, water cultivation: after 3 days of water body disinfection, fertilizer is uniformly sprayed on the water surface after being dissolved by water, topdressing can be continuously carried out for 1 time every day until the transparency of pool water is about 25cm, and after water fertilization, a probiotic preparation is applied 2 days before seedling placement to keep the balance of the water body micro-ecological environment;
s3, seed putting: when the water temperature is stable above 18 ℃, putting the penaeus vannamei boone into a fish pond, then putting the tilapia, before putting the tilapia, adopting a disinfection solvent to disinfect the tilapia, putting the fish fries into water added with the disinfection solvent, soaking for 10-20 minutes, putting the fish fries 3-4 times and the shrimp fries 5-7 times into a pond water body, wherein the time interval of putting the fish fries is 5-10 days, the time interval of putting the shrimp fries is 3-6 days, starting an aerator after 3 months of putting the fries, and the aeration time is 3-5 hours;
s4, seedling culture: feeding the shrimp fries 2 days after the shrimp fries are put into the pond, feeding 30-50 g of the feed for every 10 ten thousand shrimp fries 5 days before, feeding for 4 times every day, and increasing the feeding amount by 20 percent every day; and after 2-3 days of first fry feeding, feeding fish feed to the pond water body according to the daily feeding amount of 2-7% of fish weight, and after continuously feeding for 30-40 days, feeding the fish and shrimp mixed feed to the pond water body.
2. The method for mixed culture of tilapia and penaeus vannamei according to claim 1, which is characterized in that: the fertilizer used in the water body cultivation is a mixture of urea, a nitrogenous fertilizer, a phosphate fertilizer, a potash fertilizer and calcium carbonate, 3 parts of urea, 3 parts of nitrogenous fertilizer, 3 parts of phosphate fertilizer, 3 parts of potash fertilizer and 3 parts of calcium carbonate are taken, and the materials are put into a stirrer to be fully stirred and uniformly mixed.
3. The method for mixed culture of tilapia and penaeus vannamei according to claim 1, which is characterized in that: the fish and shrimp mixed feed consists of fish feed and shrimp feed, and the ratio of the fish feed to the shrimp feed is 3: 1.
4. The method for mixed culture of tilapia and penaeus vannamei according to claim 3, characterized in that: preparing the fish and shrimp mixed feed, weighing the fish feed and the shrimp feed according to a proportion, adding water into the weighed fish feed, stirring, adding the shrimp feed after stirring for 10 minutes, and continuing stirring for 15 minutes to obtain the fish and shrimp mixed feed.
5. The method for mixed culture of tilapia and penaeus vannamei according to claim 3, characterized in that: the fish feed consists of the following components: 7-12 parts of fish meal, 5-15 parts of wheat bran, 10-15 parts of wheat bran powder, 15-21 parts of corn protein powder, 15-21 parts of bean cake protein powder, 3-6 parts of earthworm powder, 4-6 parts of cinnamon powder, 3-5 parts of a vitamin mixture and 1.5-3.5 parts of a mineral additive.
6. The method for mixed culture of tilapia and penaeus vannamei according to claim 5, characterized in that: the preparation method of the fish feed comprises the steps of pouring fish meal, wheat bran powder, corn protein powder and bean cake protein powder into a container, stirring, uniformly mixing, pouring the rest earthworm powder, cassia bark powder, a vitamin mixing agent and a mineral additive into the container, stirring, uniformly stirring, adding a small amount of deionized water for softening, and simultaneously stirring.
7. The method for mixed culture of tilapia and penaeus vannamei according to claim 3, characterized in that: the shrimp feed consists of the following components: 20-30 parts of corn flour, 15-35 parts of wheat bran powder, 13-26 parts of zymoprotein, 10-21 parts of silkworm chrysalis, 2-4 parts of bacillus subtilis, 1-4 parts of selenium-rich yeast and 2-3 parts of compound vitamin.
8. The method for mixed culture of tilapia and penaeus vannamei according to claim 7, which is characterized in that: the preparation method of the shrimp feed comprises the steps of pouring corn flour, wheat bran powder, enzymolysis protein and silkworm chrysalis into a container, stirring, adding a proper amount of warm water with the temperature of 35 ℃ after stirring, continuously stirring, then adding bacillus subtilis, selenium-enriched yeast and multivitamin, and continuously and uniformly mixing.
9. The method for mixed culture of tilapia and penaeus vannamei according to claim 1, which is characterized in that: the disinfection solvent adopts 20ppm potassium permanganate, 8ppm copper sulfate and a proper amount of water, potassium permanganate: copper sulfate: the ratio of water is 0.2: 0.1: 10.
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