CN105858909A - Microbial herbal composite preparation for aquiculture and preparation method thereof - Google Patents
Microbial herbal composite preparation for aquiculture and preparation method thereof Download PDFInfo
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- CN105858909A CN105858909A CN201610211549.4A CN201610211549A CN105858909A CN 105858909 A CN105858909 A CN 105858909A CN 201610211549 A CN201610211549 A CN 201610211549A CN 105858909 A CN105858909 A CN 105858909A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The invention relates to a microbial herbal composite preparation for aquiculture and a preparation method thereof. The microbial herbal composite preparation for aquiculture is composed of the following raw materials in parts by weight: 5-30 parts of coptis, 5-30 parts of humifuse euphorbia herb, 5-30 parts of pomegranate rind, 5-30 parts of dark plum, 5-30 parts of Bacillus licheniformis powder, 5-30 parts of Bacillus subtilis powder and 5-30 parts of Bacillus amyloliquefaciens powder. The preparation method comprises the following steps: 1) respectively drying the coptis, humifuse euphorbia herb, pomegranate rind and dark plum at 50-60 DEG C for 2 hours, carrying out superfine grinding, and passing through a 60-80-mesh analyzer for later use; 2) respectively centrifuging the fermented Bacillus licheniformis, Bacillus subtilis and Bacillus amyloliquefaciens, and drying to obtain bacterium powder for later use; and 3) adding the pulverized coptis, humifuse euphorbia herb, pomegranate rind and dark plum powder with the Bacillus licheniformis powder, Bacillus subtilis powder and Bacillus amyloliquefaciens powder, adding the mixture into a mixing machine, and sufficiently mixing to obtain the microbial herbal composite preparation. The microbial herbal composite preparation for aquiculture is used for regulating the water quality, controlling diseases, lowering the death and culling rate and enhancing the economic benefit in the aquiculture process.
Description
Technical field
The present invention relates to aquaculture field, a kind of microorganism draft compound formulation used for aquiculture and preparation method.
Background technology
Along with China's culture fishery is fast-developing, the particularly raising of intensive culture degree causes the management of breeding water body water quality
Become more and more difficult, the problem adding aquatic animal kind matter and the aspect such as seedling quality, feed nutrition so that aquaculture
The disease incidence of animal is gradually increasing, and causes huge economic loss to raiser.For a long time, prevent and treat Aquatic product move
The traditional method of thing disease is all by using antibiotic, chemical drugs, pesticide material to realize, although they are right in a large number
The disease of aquatic animal has certain curative effect, but also brings along that antibiotic is developed immunity to drugs by pathogen, medicine is at aquatic products simultaneously
Middle residual, pesticide to the pollution of environment, destroy the problems such as breeding water body ecological balance.Development and people couple along with green economy
On the raising that food quality requires, ecologic breeding and healthy aquaculture the most prominent the most referred position.
Microbial ecological agent is the important technical realizing aquatic ecological culturing, can be divided into two classes according to purposes: a class is internal
Tiny ecosystem modifying agent, is added in feedstuff, in order to improve the composition of microbiologic population in cultivation subject, apply more having
Lactic acid bacteria, bacillus cereus, yeast, EM bacterium etc.；Another kind of is water quality Tiny ecosystem modifying agent, is rendered to breeding water body ring
With improvement substrate or water quality in border, mainly there are photosynthetic bacteria, bacillus cereus, nitrobacteria, denitrifying bacteria, EM bacterium etc..Micro-
Ecological agent reaches to prevent and treat the purpose of aquatic animal disease by " fungus treatment ", on the one hand, beneficial microbe is lived by metabolism
The poisonous and harmful substances such as dynamic degradation of organic substances, ammonia nitrogen, nitrite nitrogen, hydrogen sulfide, purifying aquatic water environment, metabolite again can
Suppress or kill pathogen, in breeding water body and cultivated animals intestinal, compete nutrition with pathogen simultaneously, occupy the Ecological niche,
Form dominant microflora, reach the purpose of controlling disease；On the other hand, beneficial microbe, by producing multiple digestive enzyme, helps egg
White matter, carbohydrate and lipid metabolism, improve efficiency of feed utilization and conversion ratio, reaches to reduce resource consumption and the purpose of volume increase.
Herbaceous plant is applied to aquaculture to be had natural sex, multifunctionality, nontoxic secondary residual, is not likely to produce the chemistry such as Drug resistance
The unrivaled advantage of synthetic drug.Herbaceous plant comes from the Nature, and they generally contain protein, saccharide, starch, fiber
Element, resin, chlorophyll and inorganic salt etc., some plants also rich in alkaloid, glycoside, flavonoid, volatile oil, organic acid,
The bioactive ingredients such as aminoacid, vitamin and enzyme.This kind of herbaceous plant be applied to aquaculture be possible not only to have additional nutrients,
Strong liver protect dirty, strengthen immunologic function and anti-stress ability, help sick body eliminating pathogenic factor for supporting vital QI and consolidate, simultaneously to malignant bacteria, fungus,
Virus and parasite also have the strongest suppression, blocking-up and killing action, help to realize the target of healthy aquaculture.
Summary of the invention
It is an object of the invention to the deficiency overcoming prior art to exist, and provide a kind of microorganism draft used for aquiculture compound system
Agent and preparation method, be a kind of regulation and control water quality, the microorganism draft compound formulation of controlling disease and system in aquaculture process
Preparation Method.
It is an object of the invention to complete by following technical solution.This microorganism draft compound formulation used for aquiculture, it
It is made up of the raw material of following weight: Rhizoma Coptidis 5～30 parts, Herba Euphorbiae Humifusae 5～30 parts, Pericarpium Granati 5～30 parts, Fructus Mume 5～30 parts, lichens
Bacillus cereus powder 5～30 parts, bacillus subtilis mycopowder 5～30 parts, bacillus amyloliquefaciens powder 5～30 parts.
As preferably, described Bacillus licheniformis powder, bacillus subtilis mycopowder, the total viable count of bacillus amyloliquefaciens powder are
≥5×109CFU/g。
As preferably, it is made up of the raw material of following weight: Rhizoma Coptidis 25 parts, Herba Euphorbiae Humifusae 25 parts, Pericarpium Granati 13 parts, Fructus Mume
10 parts, 9 parts of Bacillus licheniformis powder, bacillus subtilis mycopowder 9 parts, 9 parts of bacillus amyloliquefaciens powder.Wherein lichens spore
Bacillus powder, bacillus subtilis mycopowder, the total viable count of bacillus amyloliquefaciens powder are 8～10 × 109CFU/g。
As preferably, it is made up of the raw material of following weight: Rhizoma Coptidis 28 parts, Herba Euphorbiae Humifusae 15 parts, Pericarpium Granati 20 parts, Fructus Mume
10 parts, 9 parts of Bacillus licheniformis powder, bacillus subtilis mycopowder 9 parts, 9 parts of bacillus amyloliquefaciens powder, wherein lichens spore
Bacillus powder, bacillus subtilis mycopowder, the total viable count of bacillus amyloliquefaciens powder are 8～10 × 109CFU/g。
The preparation method of this microorganism draft compound formulation used for aquiculture of the present invention, the method specifically comprises the following steps that
1), Rhizoma Coptidis, Herba Euphorbiae Humifusae, Pericarpium Granati, Fructus Mume are dried 2 hours at 50～60 DEG C, micronizing, cross 60～80
Mesh analysis is sieved, standby；
2) Bacillus licheniformis that, fermentation completed respectively, bacillus subtilis, bacillus amyloliquefaciens are centrifuged post-drying and become bacterium
Powder, standby；
3), by the Rhizoma Coptidis crushed, Herba Euphorbiae Humifusae, Pericarpium Granati, Fructus Mume powder and Bacillus licheniformis powder, bacillus subtilis mycopowder,
Bacillus amyloliquefaciens powder is sufficiently mixed in adding mixer, prepares microorganism draft compound formulation.
The invention have the benefit that and the invention provides a kind of microorganism draft compound formulation used for aquiculture and preparation method,
For aquaculture process regulating and controlling water quality, controlling disease, reduce death rate, increase economic efficiency.
Detailed description of the invention
Below in conjunction with embodiment the present invention done detailed introduction:
This microorganism draft compound formulation used for aquiculture of the present invention, it is made up of the raw material of following weight: Rhizoma Coptidis
5～30 parts, Herba Euphorbiae Humifusae 5～30 parts, Pericarpium Granati 5～30 parts, Fructus Mume 5～30 parts, Bacillus licheniformis powder 5～30 parts, hay bud
Spore bacillus powder 5～30 parts, bacillus amyloliquefaciens powder 5～30 parts.Described Bacillus licheniformis powder, bacillus subtilis mycopowder,
The total viable count of bacillus amyloliquefaciens powder is >=5 × 109CFU/g。
Described bacterial strain is the most in the preservation of Chinese Typical Representative culture culture presevation administrative center, wherein Bacillus licheniformis HZW3, preservation
Number CCTCC NO:M 2014480；Bacillus subtilis HZW1, preserving number CCTCC NO:M 2014479；Solve starch spore
Bacillus HZW4, preserving number CCTCC NO:M 2014481.
The preparation method of this microorganism draft compound formulation used for aquiculture of the present invention, the method specifically comprises the following steps that
1), Rhizoma Coptidis, Herba Euphorbiae Humifusae, Pericarpium Granati, Fructus Mume are dried 2 hours at 50～60 DEG C, micronizing, cross 60～80
Mesh analysis is sieved, standby；
2) Bacillus licheniformis that, fermentation completed respectively, bacillus subtilis, bacillus amyloliquefaciens are centrifuged post-drying and become bacterium
Powder, standby；
3), by the Rhizoma Coptidis crushed, Herba Euphorbiae Humifusae, Pericarpium Granati, Fructus Mume powder and Bacillus licheniformis powder, bacillus subtilis mycopowder,
Bacillus amyloliquefaciens powder is sufficiently mixed in adding mixer, prepares microorganism draft compound formulation.
Microbial ecological agent is used by the present invention with herbal composition, and wherein, microbial ecological agent can suppress the growth of harmful pathogen
Breeding, by beneficial microorganism metabolism, can reduce nutrient substance superfluous in water and other poisonous and harmful substance, to going
Work is substantially regulated except the organic matter in breeding water body, ammonia nitrogen and nitrite nitrogen, reduction BOD and COD, increase dissolved oxygen aspect have
With, also can regulate the pH value of water body simultaneously, promote Nitrogen In Sediment and the release of phosphorus, the most planktonic growth.Draft
It is little and without drug-fast advantage that plant has natural sex, multifunctionality, toxic and side effects, is that instead of antibiotic, chemicals and agriculture
The preferable aquatic medicine of medicine class material.It not only can improve the breeding production level of aquatic animal, and can reduce aquatic animal
The generation of disease, stops some explosive, intractable difficult miscellaneous diseases, fundamentally solves water quality deterioration, can also significantly carry simultaneously
The immunologic function of high aquatic animal, the quality of aquatic products improved.The present invention can effectively help to realize ecologic breeding and healthy aquaculture
Target.
Using method of the present invention is:
(1) directly or uniformly shed in breeding water body, consumption 0.2～1.0kg/ (mu rice) after being dissolved in water；
(2) mixed feeding, feedstuff per ton adds 0.5～1.5kg, within every 10 days, is the most uniformly mixed in feedstuff and feeds.
Embodiment 1:
A kind of microorganism draft compound formulation preventing and treating the bacillary pathogenic bacterium such as vibrio, Aeromonas, it is by the raw material of following weight
Composition: Rhizoma Coptidis 28 parts, Herba Euphorbiae Humifusae 15 parts, Pericarpium Granati 20 parts, Fructus Mume 10 parts, 9 parts of Bacillus licheniformis powder, hay bud
9 parts of spore bacillus powder, 9 parts of bacillus amyloliquefaciens powder.Wherein Bacillus licheniformis powder, bacillus subtilis mycopowder, solution starch bud
The total viable count of spore bacillus powder is (8～10) × 109CFU/g。
Embodiment 2: it is made up of the raw material of following weight: Rhizoma Coptidis 25 parts, Herba Euphorbiae Humifusae 25 parts, Pericarpium Granati 13 parts, Fructus Mume
10 parts, 9 parts of Bacillus licheniformis powder, bacillus subtilis mycopowder 9 parts, 9 parts of bacillus amyloliquefaciens powder.Wherein lichens spore
Bacillus powder, bacillus subtilis mycopowder, the total viable count of bacillus amyloliquefaciens powder are 8～10 × 109CFU/g。
, there is generally row pool phenomenon, cultivated animals mortality rate in culture of Penaeus vannamei base, Jiangsu Rudong when entering cultivation middle and late stage
Crossing 20%, as long as being that early stage used all users of product of the present invention all not occur arranging pool phenomenon, prawn growth is normal, growing way
Preferably, and sick shrimp aquaculture family uses the present embodiment to treat, and feedstuff per ton adds 2kg, and next day is dead compared with the comparison shrimp pool
Rate substantially reduces, and the most no longer occurs the phenomena of mortality after 3 days.Afterwards, sick shrimp, dead shrimp are analyzed as this through Aquatic product research institution
Generally row pool phenomenon is mainly caused by the bacillary pathogenic bacterium such as vibrio, Aeromonas.
Taking Penaeus vannamei a collection of, specification is body length 8.0 ± 0.1cm, and body weight 4.5～5.5g is in a good state of health.Test shrimp with
Machine is divided into test group and matched group two groups, and often group sets 3 repetitions, each repetition 30 tail, totally 180 tail.Raise in 40cm ×
In the glass aquarium of 30cm × 25cm (depth of water 20cm), test group adds 2ppm microorganism of the present invention draft compound formulation
(remaining condition: water temperature 25～28 DEG C, dissolved oxygen >=5.0, salinity 10, pH 7.8～8.2, feedstuff feeding amount all keeps with matched group
Unanimously) test period is 30 days, samples at the 0th, 15,30 days respectively, extracts test group and the blood of matched group shrimp respectively
Liquid, hepatic homogenate carry out relevant phenol oxidase (PO), lysozyme (LSZ) and the work of superoxide dismutase (SOD)
Power measures, and the results are shown in Table 1.After off-test, every shrimp is carried out weighing body weight, calculate the relative weight gain of two groups of shrimps according to formula
Rate: body weight increase rate=(end average weight just average weight)/first average weight, the results are shown in Table 2.
PO, LSZ, SOD vigor in table 1 Penaeus vannamei tissue
Table 2 Penaeus vannamei body weight increase rate after 30 days
| Packet | Matched group | Test group |
| Body weight increase rate (%) | 28.66 | 35.74 |
Test shows, the present invention can aoxidize by improving the superoxide dismutase (SOD) in prawn liver and the phenol in serum
Enzyme (PO), three kinds of enzyme activities of lysozyme (LSZ) significantly improve the non-specific immunity of prawn, and strong liver is protected dirty, strengthens it
Defence capability to disease, can effectively facilitate prawn growth simultaneously.
Take the Wai Tangshui of certain culture of Penaeus vannamei severe contamination, be divided into test group and matched group two groups after mix homogeneously, often organize work
3 repetitions, each repetition 20L water, altogether 120L water.Test group adds microorganism draft of the present invention by 1ppm consumption and is combined
Preparation (remaining condition: water temperature 25～28 DEG C, dissolved oxygen >=5.0, salinity 10, pH 7.8～8.2 all keeps consistent with matched group) examination
The time of testing is 30 days, is sampled at the 0th, 5,10,20,30 days respectively, measures the COD of each repetition water sample
(COD), total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH3-N), nitrite nitrogen (NO2-N) index, concrete outcome is shown in Table
3。
Table 3 water quality detection index
Unit: mg/L
Test shows, microorganism draft compound formulation energy efficient degradation Organic substance of the present invention, nitrogen phosphorus and ammonia nitrogen, nitrite nitrogen etc. are poisonous
Harmful substance, purifying aquatic water, improves cultivated animals living environment.Use the present invention can accelerate water body self-regeneration speed,
Water body environment change, because of organic corrupt produced toxin and harmful substances such as residual feed and excretas, is played buffering by degraded in time
Effect, reduce the cultivated animals stress to surrounding, promote cultivated animals healthy growth, strengthen autoimmune function with
Resist the invasion and attack of pathogenic microorganism, reduce sickness rate.
Above the specific embodiment of the present invention has been described in detail, but it has been intended only as example, the present invention be not restricted to
The specific embodiment of upper description.Every any equivalent modifications that the present invention is carried out and substitute the most all among scope of the invention,
Therefore in the equivalents made without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (5)
1. a microorganism draft compound formulation used for aquiculture, it is characterised in that: it is made up of the raw material of following weight: yellow
Even 5～30 parts, Herba Euphorbiae Humifusae 5～30 parts, Pericarpium Granati 5～30 parts, Fructus Mume 5～30 parts, Bacillus licheniformis powder 5～30 parts, withered
Grass bacillus cereus powder 5～30 parts, bacillus amyloliquefaciens powder 5～30 parts.
Microorganism draft compound formulation used for aquiculture the most according to claim 1, it is characterised in that: described lichens bud
Spore bacillus powder, bacillus subtilis mycopowder, the total viable count of bacillus amyloliquefaciens powder are >=5 × 109CFU/g。
Microorganism draft compound formulation used for aquiculture the most according to claim 1, it is characterised in that: it is by following weight
Raw material composition: Rhizoma Coptidis 28 parts, Herba Euphorbiae Humifusae 15 parts, Pericarpium Granati 20 parts, Fructus Mume 10 parts, 9 parts of Bacillus licheniformis powder,
Bacillus subtilis mycopowder 9 parts, 9 parts of bacillus amyloliquefaciens powder, wherein Bacillus licheniformis powder, bacillus subtilis mycopowder, solution
The total viable count of bacillus amyloliquefaciens powder is 8～10 × 109CFU/g。
Microorganism draft compound formulation used for aquiculture the most according to claim 1, it is characterised in that: it is by following heavy
Amount raw material composition: Rhizoma Coptidis 25 parts, Herba Euphorbiae Humifusae 25 parts, Pericarpium Granati 13 parts, Fructus Mume 10 parts, 9 parts of Bacillus licheniformis powder,
Bacillus subtilis mycopowder 9 parts, 9 parts of bacillus amyloliquefaciens powder, wherein Bacillus licheniformis powder, bacillus subtilis mycopowder, solution
The total viable count of bacillus amyloliquefaciens powder is 8～10 × 109CFU/g。
5. the method preparing microorganism draft compound formulation used for aquiculture as claimed in claim 1, it is characterised in that:
The method specifically comprises the following steps that
1), Rhizoma Coptidis, Herba Euphorbiae Humifusae, Pericarpium Granati, Fructus Mume are dried 2 hours at 50～60 DEG C, micronizing, cross 60～80
Mesh analysis is sieved, standby；
2) Bacillus licheniformis that, fermentation completed respectively, bacillus subtilis, bacillus amyloliquefaciens are centrifuged post-drying and become bacterium
Powder, standby；
3), by the Rhizoma Coptidis crushed, Herba Euphorbiae Humifusae, Pericarpium Granati, Fructus Mume powder and Bacillus licheniformis powder, bacillus subtilis mycopowder,
Bacillus amyloliquefaciens powder is sufficiently mixed in adding mixer, prepares microorganism draft compound formulation.
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| CN201610211549.4A CN105858909A (en) | 2016-04-05 | 2016-04-05 | Microbial herbal composite preparation for aquiculture and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106361955A (en) * | 2016-08-29 | 2017-02-01 | 佛山市艳晖生物科技有限公司 | Preparation method of Chinese herbal medicinal active fermentation broth for preventing bacterial diseases in fishes and shrimps |
| CN106472393A (en) * | 2016-09-28 | 2017-03-08 | 吕子含 | A kind of shrimp culture pond water quality and algae automatic control and management system and its control method |
| CN108077656A (en) * | 2018-02-09 | 2018-05-29 | 山东绿增农业有限公司 | A kind of high-performance aquaculture organisms fodder grass and preparation method thereof |
| CN108641976A (en) * | 2018-04-13 | 2018-10-12 | 北京天诚众合科技发展有限公司 | A kind of complex microorganism water purification agent and preparation method thereof |
| CN111100830A (en) * | 2020-03-09 | 2020-05-05 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
| CN112047492A (en) * | 2020-09-04 | 2020-12-08 | 镇江立嘉农业发展有限公司 | Fish culture sewage treatment preparation and application method thereof |
-
2016
- 2016-04-05 CN CN201610211549.4A patent/CN105858909A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106361955A (en) * | 2016-08-29 | 2017-02-01 | 佛山市艳晖生物科技有限公司 | Preparation method of Chinese herbal medicinal active fermentation broth for preventing bacterial diseases in fishes and shrimps |
| CN106472393A (en) * | 2016-09-28 | 2017-03-08 | 吕子含 | A kind of shrimp culture pond water quality and algae automatic control and management system and its control method |
| CN108077656A (en) * | 2018-02-09 | 2018-05-29 | 山东绿增农业有限公司 | A kind of high-performance aquaculture organisms fodder grass and preparation method thereof |
| CN108641976A (en) * | 2018-04-13 | 2018-10-12 | 北京天诚众合科技发展有限公司 | A kind of complex microorganism water purification agent and preparation method thereof |
| CN111100830A (en) * | 2020-03-09 | 2020-05-05 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
| CN112047492A (en) * | 2020-09-04 | 2020-12-08 | 镇江立嘉农业发展有限公司 | Fish culture sewage treatment preparation and application method thereof |
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